Starting /dee2/code/volunteer_pipeline.sh SRR13695477 current disk space = 3050339078144 free memory = 1582355860 SRR13695477 SRAfilesize efda19a7fbbcd9e1967214190d5e3644 SRR13695477.sra SRR13695477.sra file validated SRR13695477 is paired end SRR13695477 is conventional basespace SRR13695477 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695477_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6715 37.0 37.0 37.0 37.0 37.0 2 36.39125 37.0 37.0 37.0 37.0 37.0 3 36.643 37.0 37.0 37.0 37.0 37.0 4 36.654 37.0 37.0 37.0 37.0 37.0 5 36.547 37.0 37.0 37.0 37.0 37.0 6 36.668 37.0 37.0 37.0 37.0 37.0 7 36.5005 37.0 37.0 37.0 37.0 37.0 8 36.5695 37.0 37.0 37.0 37.0 37.0 9 36.5255 37.0 37.0 37.0 37.0 37.0 10-14 36.5711 37.0 37.0 37.0 37.0 37.0 15-19 36.5018 37.0 37.0 37.0 37.0 37.0 20-24 36.475199999999994 37.0 37.0 37.0 37.0 37.0 25-29 36.409499999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.4135 37.0 37.0 37.0 37.0 37.0 35-39 36.4169 37.0 37.0 37.0 37.0 37.0 40-44 36.355900000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.2988 37.0 37.0 37.0 37.0 37.0 50-54 36.313 37.0 37.0 37.0 37.0 37.0 55-59 36.2764 37.0 37.0 37.0 37.0 37.0 60-64 36.284000000000006 37.0 37.0 37.0 37.0 37.0 65-69 36.240899999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.26559999999999 37.0 37.0 37.0 37.0 37.0 75-79 36.211 37.0 37.0 37.0 37.0 37.0 80-84 36.157799999999995 37.0 37.0 37.0 37.0 37.0 85-89 36.160799999999995 37.0 37.0 37.0 37.0 37.0 90-94 36.1514 37.0 37.0 37.0 37.0 37.0 95-99 36.0391 37.0 37.0 37.0 37.0 37.0 100-104 36.029700000000005 37.0 37.0 37.0 37.0 37.0 105-109 36.03869999999999 37.0 37.0 37.0 37.0 37.0 110-114 36.0172 37.0 37.0 37.0 37.0 37.0 115-119 36.0238 37.0 37.0 37.0 37.0 37.0 120-124 35.980999999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.9247 37.0 37.0 37.0 37.0 37.0 130-134 35.919799999999995 37.0 37.0 37.0 37.0 37.0 135-139 35.85119999999999 37.0 37.0 37.0 37.0 37.0 140-144 35.8279 37.0 37.0 37.0 37.0 37.0 145-149 35.580799999999996 37.0 37.0 37.0 37.0 37.0 150-151 35.47325 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 3.0 22 4.0 23 5.0 24 3.0 25 4.0 26 6.0 27 12.0 28 14.0 29 22.0 30 30.0 31 35.0 32 44.0 33 73.0 34 116.0 35 288.0 36 2954.0 37 385.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.074999999999996 13.875000000000002 10.075000000000001 36.975 2 21.50564617314931 14.629861982434129 33.92722710163112 29.937264742785448 3 19.325 17.825 26.674999999999997 36.175000000000004 4 22.875 25.174999999999997 22.975 28.975 5 24.025 28.999999999999996 25.124999999999996 21.85 6 22.25 34.1 23.525 20.125 7 17.0 29.7 37.2 16.1 8 17.625 28.825 31.25 22.3 9 17.075000000000003 24.675 35.099999999999994 23.150000000000002 10-14 19.675 30.085 27.295 22.945 15-19 20.27 28.854999999999997 28.185 22.689999999999998 20-24 20.185 28.9 28.26 22.655 25-29 19.785 29.099999999999998 28.025 23.09 30-34 19.66 29.935000000000002 27.375 23.03 35-39 19.985 29.189999999999998 27.6 23.225 40-44 20.96 28.53 26.995 23.515 45-49 20.575 28.389999999999997 27.46 23.575 50-54 20.095 28.875 27.365000000000002 23.665 55-59 19.68 28.73 28.050000000000004 23.54 60-64 19.555 29.195 26.655 24.595 65-69 20.43 29.01 26.8 23.76 70-74 21.36 29.145 26.224999999999998 23.27 75-79 19.695 27.310000000000002 28.050000000000004 24.945 80-84 21.645 28.499999999999996 27.195000000000004 22.66 85-89 20.599999999999998 28.060000000000002 27.355 23.985 90-94 21.995 27.79 26.035000000000004 24.18 95-99 20.005 28.655 27.12 24.22 100-104 20.845 28.18 26.939999999999998 24.035 105-109 21.325 27.77 27.955000000000002 22.95 110-114 20.830000000000002 27.725 27.589999999999996 23.855 115-119 20.75 28.315 26.935 24.0 120-124 20.68 27.715 27.32 24.285 125-129 19.580000000000002 29.56 26.200000000000003 24.66 130-134 21.44 27.700000000000003 26.474999999999998 24.385 135-139 21.23 28.395 25.814999999999998 24.560000000000002 140-144 21.025 28.07 25.840000000000003 25.064999999999998 145-149 21.27 27.265 26.41 25.055 150-151 21.212500000000002 27.275 26.224999999999998 25.2875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 0.5 7 0.0 8 0.5 9 1.0 10 2.0 11 1.5 12 1.0 13 1.5 14 0.5 15 0.5 16 1.0 17 0.5 18 0.0 19 0.5 20 2.0 21 3.0 22 1.5 23 1.0 24 2.0 25 1.0 26 1.0 27 3.0 28 8.0 29 8.5 30 12.0 31 31.5 32 37.5 33 38.5 34 69.5 35 102.0 36 117.0 37 124.0 38 153.0 39 178.0 40 172.5 41 197.0 42 221.0 43 222.5 44 227.0 45 214.5 46 222.5 47 222.5 48 211.5 49 210.0 50 178.5 51 136.0 52 115.5 53 102.0 54 93.0 55 86.0 56 64.0 57 50.5 58 37.0 59 32.5 60 30.0 61 19.5 62 10.5 63 6.0 64 3.5 65 0.5 66 1.5 67 1.5 68 1.0 69 0.5 70 1.0 71 1.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.375 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.5 #Duplication Level Percentage of deduplicated Percentage of total 1 68.19548872180451 45.35 2 20.18796992481203 26.85 3 7.330827067669173 14.625 4 2.518796992481203 6.7 5 1.2406015037593985 4.125 6 0.3383458646616541 1.35 7 0.11278195488721805 0.525 8 0.03759398496240602 0.2 9 0.0 0.0 >10 0.03759398496240602 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGATTTTTTCCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATA 11 0.27499999999999997 No Hit CAGCAATTCAGGAAGAAATATGACAAGGAGTAGTAGTGTGGATGTTGTTG 8 0.2 No Hit CTCTAGAATACAAATCAAATGTACTAGCAGTCTTGGGAATAGAAGCTGAC 7 0.17500000000000002 No Hit CTCAGATCGGATTGCTGCTGAAGTAGCTAGAGCTTTCTCCAAACGCCTCT 7 0.17500000000000002 No Hit AGGGGCAGCAGCAACAGCAAACTTGCTAGGATCCTCCAGGAACTCTTTCA 7 0.17500000000000002 No Hit CTCACCGAGTGGCCCGCCAGCAATTCTGTAACCCTCAACGGCACCCATCA 6 0.15 No Hit CAACGTCGCAAAAAACCAGCAAAAATTCTTAACATTATTTTTAGAAATCC 6 0.15 No Hit GCTCCCACGACCGTCTCAGCAGCTCCCGCAAAGTGACCCTTCTCTGGTGC 6 0.15 No Hit ACCGGTCTTCATTTACATGTGTCAACGGGTGAACAACCTGCAATTTCTCG 6 0.15 No Hit CACCTTTCCACTCTCCAAGTAAGGCTTCAGTTTGTCCAAGACAGAGCCAT 6 0.15 No Hit GGCATTACATCAAAGCAAAAACCTATATAACCCCTTTCTAGGCCCTATAA 6 0.15 No Hit CTGGAATATGTCAGCCATCCACTTGACTGAATTCTAGAGCATTTGTATCT 6 0.15 No Hit CTCCTAACAGTATTGGTTTCGCCTTCATTAGCTTCCATGATCCTTGCCAT 6 0.15 No Hit AGCAGAGATAACAGTGTTGAGGTCAAAACGAAGATATCCTGCCTTGAGAC 6 0.15 No Hit GGAGGTGTGGCGATGCCTCTTGGGTTTCTTGATGCCATTCTTGTGAGCTT 5 0.125 No Hit GCTAGGGCTGTGTTATCAGCTGGTTTTGAGATGGTTAGACCTTTTGGGAG 5 0.125 No Hit CTTGACTACAGATACCATAAGCACCGCAGCAACACTGGGTCACATCATTT 5 0.125 No Hit CTCCAATATTACAGTCGCAGGAAAAGGCATCAGCTAAGAGAGCTTACAGG 5 0.125 No Hit AGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATAT 5 0.125 No Hit AAAATTAGAATCGTCTTAGCTTATGGAATTATGCTTTTCCCAGAGATGGC 5 0.125 No Hit GGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTAGAATTACTGGCAC 5 0.125 No Hit TTCTACATGACTGCAAATGGTACCACATCACGGTACTCGTAGCACAAGGG 5 0.125 No Hit GGGAGCAGTACGCATATCCTTCCCTTAAAAAGATAACTGAAAGACAAAAA 5 0.125 No Hit GGGCAGGAGAAAGAAAATCAACCGACAGCTAGTAATTGAAATAACATGGC 5 0.125 No Hit GGAGTGTTCTAAGAGAAAAAGAAGACACAACGAAGGGTCTTAAAACATTT 5 0.125 No Hit GCAATAAAGCTGATGCACTGCACTTGACGCGTGTTGTCGAATCCGATTAT 5 0.125 No Hit TCCTCCCATGGGTGACGGTATAGAGGCTGTTTCAATCTCTTCTGGTCAAA 5 0.125 No Hit GGGGAGGAAAACGGCAGAGACAGAATTAAAAGCAAAGTACTTCGATCAAG 5 0.125 No Hit GCATCCTGGACAAGACGTTCACTAACTGCATCAAGTGCACTAGTGGCCTC 5 0.125 No Hit AAGTGCTGTCCACAGTAGAGTTCATTCCAAAGCTTCTTGTATTTCCCAAA 5 0.125 No Hit CCCCGCTTGTCAGGACAGCTTTGGCACCTCCTTCAATCCTGGTTTGGGAA 5 0.125 No Hit TGGGCTTTCTTGCTGAAACACGCCATCCTTGAGCCATCTTTCAGGTTTGT 5 0.125 No Hit CTAGCAAAGTAGGGGGCAGCTTCATCTTCAACAATGCCTTGCCGTACTCA 5 0.125 No Hit GGGGAGGTATAACAAAATCTATGAATATACAAATATGCTAGAAGAGTTAT 5 0.125 No Hit CTTTATTTTTCTTTCAGGTGGAATAGTAAACCATTGAAGAACTTATCACC 5 0.125 No Hit CTCTTGCCCCCTCCATTTCCTACACGTCCCACTAGCCCCTGGAAGAGTTA 5 0.125 No Hit CATCAATATCTTCTTGCTTCGCAAGTTCAGCACAATTGCCTCCATTTACA 5 0.125 No Hit CCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATC 5 0.125 No Hit CCAGGAACGAAGTTAGTGGCGTATGCCCAAGCATTGTTGGCCACTGGATC 5 0.125 No Hit CAGGAAGAAATATGACAAGGAGTAGTAGTGTGGATGTTGTTGTTAGACAC 5 0.125 No Hit TCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGT 5 0.125 No Hit AATCCCTATTTCTGCTTTGCGGGACTCGACAAACCCAAGTAGAAGTGGAC 5 0.125 No Hit GTTGAATCGAACCACCACCGGGTAGCGGCTCCTGGGGTCCTGATCAACAG 5 0.125 No Hit CCTCTGGTTACTAAGATGTTTCAGTTCGCCAGGTTGTCTCTTGCCTGCCC 5 0.125 No Hit GTACGAAACAAAAAAGAAAAAAGAGCAATCGTCTAAGCTTCATAAACTTT 5 0.125 No Hit CCTCGGGGTACAACGGAGAAGCCAAACCTAAAAACTAACAGAATCTTGCT 5 0.125 No Hit CTTTTTTAAAATGCATACAAATACAGTTACAAACACAGTACCAAAGGAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.15000000000000002 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.275 0.0 0.0 0.0 0.0 84-85 0.3125 0.0 0.0 0.0 0.0 86-87 0.4375 0.0 0.0 0.0 0.0 88-89 0.5874999999999999 0.0 0.0 0.0 0.0 90-91 0.7375 0.0 0.0 0.0 0.0 92-93 0.9 0.0 0.0 0.0 0.0 94-95 1.0375 0.0 0.0 0.0 0.0 96-97 1.175 0.0 0.0 0.0 0.0 98-99 1.475 0.0 0.0 0.0 0.0 100-101 1.775 0.0 0.0 0.0 0.0 102-103 2.05 0.0 0.0 0.0 0.0 104-105 2.2 0.0 0.0 0.0 0.0 106-107 2.425 0.0 0.0 0.0 0.0 108-109 2.8625 0.0 0.0 0.0 0.0 110-111 3.1875 0.0 0.0 0.0 0.0 112-113 3.4375 0.0 0.0 0.0 0.0 114-115 3.6875 0.0 0.0 0.0 0.0 116-117 4.05 0.0 0.0 0.0 0.0 118-119 4.625 0.0 0.0 0.0 0.0 120-121 5.2375 0.0 0.0 0.0 0.0 122-123 5.7875 0.0 0.0 0.0 0.0 124-125 6.4375 0.0 0.0 0.0 0.0 126-127 7.3875 0.0 0.0 0.0 0.0 128-129 8.1625 0.0 0.0 0.0 0.0 130-131 9.125 0.0 0.0 0.0 0.0 132-133 9.6125 0.0 0.0 0.0 0.0 134-135 10.2875 0.0 0.0 0.0 0.0 136-137 10.925 0.0 0.0 0.0 0.0 138-139 11.725000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTTGAT 10 0.006830828 145.0 145 GCCATTC 10 0.006830828 145.0 1 >>END_MODULE SRR13695477 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695477_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.133 37.0 37.0 37.0 37.0 37.0 2 36.267 37.0 37.0 37.0 37.0 37.0 3 36.164 37.0 37.0 37.0 37.0 37.0 4 36.2185 37.0 37.0 37.0 37.0 37.0 5 36.3205 37.0 37.0 37.0 37.0 37.0 6 36.1875 37.0 37.0 37.0 37.0 37.0 7 36.2825 37.0 37.0 37.0 37.0 37.0 8 36.2655 37.0 37.0 37.0 37.0 37.0 9 36.2935 37.0 37.0 37.0 37.0 37.0 10-14 36.305400000000006 37.0 37.0 37.0 37.0 37.0 15-19 36.2179 37.0 37.0 37.0 37.0 37.0 20-24 36.1939 37.0 37.0 37.0 37.0 37.0 25-29 36.19565 37.0 37.0 37.0 37.0 37.0 30-34 36.134100000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.09935 37.0 37.0 37.0 37.0 37.0 40-44 36.1248 37.0 37.0 37.0 37.0 37.0 45-49 36.062799999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.025 37.0 37.0 37.0 37.0 37.0 55-59 36.07995 37.0 37.0 37.0 37.0 37.0 60-64 36.006449999999994 37.0 37.0 37.0 37.0 37.0 65-69 35.89305 37.0 37.0 37.0 37.0 37.0 70-74 35.86364999999999 37.0 37.0 37.0 37.0 37.0 75-79 35.9465 37.0 37.0 37.0 37.0 37.0 80-84 35.9641 37.0 37.0 37.0 37.0 37.0 85-89 35.84689999999999 37.0 37.0 37.0 37.0 37.0 90-94 35.81055 37.0 37.0 37.0 37.0 37.0 95-99 35.8478 37.0 37.0 37.0 37.0 37.0 100-104 35.815149999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.8264 37.0 37.0 37.0 37.0 37.0 110-114 35.79115 37.0 37.0 37.0 37.0 37.0 115-119 35.7179 37.0 37.0 37.0 37.0 37.0 120-124 35.68295 37.0 37.0 37.0 37.0 37.0 125-129 35.6651 37.0 37.0 37.0 37.0 37.0 130-134 35.59365 37.0 37.0 37.0 37.0 37.0 135-139 35.47835 37.0 37.0 37.0 37.0 37.0 140-144 35.39335 37.0 37.0 37.0 34.6 37.0 145-149 35.28294999999999 37.0 37.0 37.0 37.0 37.0 150-151 34.9215 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 2.0 14 4.0 15 5.0 16 2.0 17 3.0 18 4.0 19 3.0 20 2.0 21 7.0 22 3.0 23 5.0 24 7.0 25 6.0 26 13.0 27 8.0 28 14.0 29 13.0 30 21.0 31 26.0 32 48.0 33 82.0 34 171.0 35 469.0 36 2808.0 37 271.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.036108324974926 22.592778335005015 13.866599799398195 26.504513540621865 2 29.4 24.525 27.950000000000003 18.125 3 21.475 28.4 30.5 19.625 4 26.125 32.1 23.075000000000003 18.7 5 26.375 35.025 21.224999999999998 17.375 6 24.075 34.5 23.025000000000002 18.4 7 23.0 22.875 35.3 18.825 8 24.6 23.200000000000003 26.3 25.900000000000002 9 23.1 26.450000000000003 27.425 23.025000000000002 10-14 24.709999999999997 28.29 25.724999999999998 21.275 15-19 24.445 28.000000000000004 26.314999999999998 21.240000000000002 20-24 24.71735867933967 27.753876938469237 26.72336168084042 20.805402701350676 25-29 23.882912184138103 28.20615461596197 26.68001000750563 21.230923192394297 30-34 24.79487692615569 28.131879127476484 26.265759455673404 20.807484490694417 35-39 24.81364750612837 27.780279153534444 26.45955275401471 20.946520586322478 40-44 25.07504502701621 28.74224534720832 25.965579347608564 20.217130278166902 45-49 24.212106053026513 28.084042021010507 26.623311655827912 21.080540270135067 50-54 24.61230615307654 27.498749374687343 26.703351675837915 21.1855927963982 55-59 24.943707780835627 28.621466099574683 25.96447335501626 20.47035276457343 60-64 24.003201760968533 27.58016909300115 27.054880184101254 21.36174896192906 65-69 23.893362676936928 27.259540839293756 27.479617866253186 21.36747861751613 70-74 24.90367775831874 27.135351513635225 27.150362772079063 20.810607955966976 75-79 24.77486491895137 27.40644386631979 27.121272763658194 20.697418451070643 80-84 24.3671835917959 27.70385192596298 26.983491745872932 20.945472736368185 85-89 24.302011407985592 27.098969278494945 27.684379065345745 20.914640248173722 90-94 24.613537445595078 28.020411226174396 26.724698584221322 20.641352744009207 95-99 23.77688844422211 28.66933466733367 26.248124062031014 21.30565282641321 100-104 24.588441330998247 27.865899424568426 27.335501626219667 20.21015761821366 105-109 24.807365155608927 27.469228459921947 27.304112879015314 20.419293505453815 110-114 25.058782330281655 27.615188353594476 27.310020511281202 20.016008804842663 115-119 24.639783870322194 28.78226936161697 26.76605963578147 19.811887132279367 120-124 25.01376032024018 28.71653740305229 26.134600950713033 20.135101325994494 125-129 25.815489293576142 28.301981188713228 27.001200720432262 18.881328797278368 130-134 25.449086815111333 27.89592194145609 26.855141356017015 19.79984988741556 135-139 25.559169377032774 27.555666750062546 26.945208906680012 19.93995496622467 140-144 26.559607784281354 27.475111311221173 26.639651808494673 19.325629096002803 145-149 26.319739804853644 26.84013009757318 27.280460345258945 19.559669752314235 150-151 27.583187390542907 26.80760570427821 26.882661996497376 18.72654490868151 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2.0 1 1.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.0 8 0.5 9 0.0 10 0.5 11 2.5 12 2.5 13 0.5 14 0.5 15 1.5 16 1.0 17 1.0 18 1.0 19 0.5 20 0.5 21 0.0 22 0.0 23 0.5 24 1.0 25 2.0 26 1.5 27 0.5 28 1.0 29 2.0 30 7.5 31 14.5 32 20.5 33 23.5 34 34.0 35 47.0 36 52.5 37 83.0 38 127.0 39 148.5 40 173.5 41 206.5 42 234.5 43 254.0 44 247.0 45 237.0 46 261.5 47 256.5 48 229.0 49 235.0 50 196.5 51 163.0 52 147.0 53 120.0 54 99.5 55 78.0 56 67.0 57 45.5 58 34.5 59 32.0 60 21.0 61 18.5 62 15.5 63 7.5 64 5.0 65 1.5 66 1.0 67 1.0 68 0.0 69 1.0 70 1.0 71 0.0 72 0.0 73 0.5 74 1.0 75 1.0 76 0.5 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.5 84 1.0 85 0.5 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 2.0 96 1.5 97 0.0 98 2.0 99 3.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.05 25-29 0.075 30-34 0.06 35-39 0.055 40-44 0.06 45-49 0.05 50-54 0.05 55-59 0.075 60-64 0.055 65-69 0.034999999999999996 70-74 0.075 75-79 0.06 80-84 0.05 85-89 0.06999999999999999 90-94 0.055 95-99 0.05 100-104 0.075 105-109 0.06999999999999999 110-114 0.055 115-119 0.06 120-124 0.075 125-129 0.06 130-134 0.075 135-139 0.075 140-144 0.055 145-149 0.075 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.77499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 69.03781355297642 46.1 2 19.655559715462374 26.25 3 6.776488206664171 13.575000000000001 4 2.73305877948334 7.3 5 1.2354923249719205 4.125 6 0.3743916136278547 1.5 7 0.11231748408835641 0.525 8 0.0 0.0 9 0.0 0.0 >10 0.07487832272557095 0.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 14 0.35000000000000003 No Hit GCTCATCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCT 11 0.27499999999999997 No Hit TTTTAGAAGAGTTCTTTGTAAGCTAATAAGTTGAAAAGAGGGACAATATT 7 0.17500000000000002 No Hit CAAGCTCTTGAATGGGAACTCAGTGGACCCTGCAGATTGGAGGCAGAGAA 7 0.17500000000000002 No Hit CTCGTTGTCTTTGGCCACCTCTTACCCAACCCTTGCTGCCGCACCACACA 7 0.17500000000000002 No Hit TTCTCGAGTAAGGAGATATCTCACATCTGAGGACTGGACGGGTCCTGAAG 6 0.15 No Hit GATTACACCAAGGAAAACTTTGAGGACCTGCCAGAGAAGTTTGATGTAGT 6 0.15 No Hit ACGTGCTCAACTTAGTTGGAGAAGTCGTATTCATATTATAAATGGGATTG 6 0.15 No Hit GATGCACTGAGGCATCTCAAGTGTTGCTTGAGCTTGAGGAGGCAAAGAAA 6 0.15 No Hit CGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTC 6 0.15 No Hit GTTGGGTCTTGTTGCTGCTGGTTTGGCTTCTGGTTCTTTTGTTCAAGTTG 6 0.15 No Hit TTGAACACTCTATACTCCAAGCTGTTTACAAAAGAAAAAAGAAGGCAGTC 6 0.15 No Hit GTCTCTTGAATTTTATCAGGGAATCTTAGGGCTTGAAATAAATGAAGAAA 6 0.15 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 6 0.15 No Hit TTGACTACTTGGGCAACCCAAGCTTGATCCACGCACAAAGCATCTTGGCC 6 0.15 No Hit CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 5 0.125 No Hit GGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAAT 5 0.125 No Hit GGCTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAG 5 0.125 No Hit GTTCATACAGTTGAGAGCATTGTTTTAAGCATCATATCAATGCTTTCTAG 5 0.125 No Hit CGTTAACCAAGATCCCATCTTCAAACAATACAGCTTGCCTCCAAATGAAT 5 0.125 No Hit ATCACTTCCACAGGGTTATGACACTCTAGTTGGTGAGCGTGGAGGCTTAC 5 0.125 No Hit ACTTTGGAGAGGTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTT 5 0.125 No Hit AATTTTTGCTGGTTTTTTGCGACGTTGGTGTTGTATTCTATAGCTCCATT 5 0.125 No Hit GATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCAT 5 0.125 No Hit GACTAGCACAAGCCTCATGCTATTTGCTGGGAGATTTGGGTTAGCCCCAT 5 0.125 No Hit AGAAAGTGCTCGAGCTCGAGAGGAAGAGGTTGCAAGCTCGTGGTGCAAGT 5 0.125 No Hit ATACAGCCAAAGGGTCGTTGATTTAGCACATCTTGTAGCCAACAAGTGGC 5 0.125 No Hit ACTAGTTTCAGTAATGTAATAGGAATTTGCATCCAAGGTGCATGCCCATG 5 0.125 No Hit CGGTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTCGAT 5 0.125 No Hit TCTCAGTCCTTGTCAACTGTGGCATCAGAAATTGCAACTGGGAGCAGCAG 5 0.125 No Hit CCTATTCTCTTCGTGAAGATGTCTTGCTGTGGAGGAAACTGTGGGTGTGG 5 0.125 No Hit TTAAACTGTATTATCCAAAGCAACCATGATTTGGAAGGGTATGTTAATCA 5 0.125 No Hit GTATTGGGCACAGGATCAAGAGAGGGGACAACAGGGATAAAAGAGTGGAG 5 0.125 No Hit CTTTCTTGTTGTGCAAATTAAAAAAAAAACCTCTAGGGTTGGGGCTTGGT 5 0.125 No Hit GCAAGCCCTTTGGACTATCTCTCGTGCCCCATCATCAGTACAGCAGGGGG 5 0.125 No Hit ATTTCCTCTCTACAGTCGTAATTTTCCAGAAGAGGGGTCAACATGGAATA 5 0.125 No Hit TGAAGCTCCAAAACCTAAGCCACCACCTATTGGACCCAAGAGAGGAACCA 5 0.125 No Hit ATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAAT 5 0.125 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 5 0.125 No Hit CTTCGCTATCTGGTCTGCCCTTTCATTTCACCATCAACACCCTCTTCTTG 5 0.125 No Hit GATGAAGTCGCCATGGGAAATACTGCAATCACCAGTTGGATCTGGAGGAG 5 0.125 No Hit CTGTTCGATTTCTTAAAGGACTGAATATTCGGTGGCAGTATGGGATTTCT 5 0.125 No Hit GCAATACTCAATGCAAAGTGCTTGAGCATCAACGCCAACAAGAATACCAA 5 0.125 No Hit GCTGTGAGAACCTAACGGCGGAGAACAGGAGGTTGCAAAAGGAAGTTCAG 5 0.125 No Hit GGGAGGAGTTATGGATAGTGGATTGTTGTCACGTGATTATCTATTGTAAC 5 0.125 No Hit TCCATGTCAAGCCCCGCCTTGGCTCTGGTGGATGAGAGAATGTCCACTGA 5 0.125 No Hit TGAGCTTCAACGATCATGATGATGGCTTCAAAGGGCGACAGAAAGCTCAG 5 0.125 No Hit TTCTGGCCTAGTACAGGTCGTGTCGACAATGTCTATGGTGACCGCAACCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.15000000000000002 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.275 0.0 0.0 0.0 0.0 84-85 0.32499999999999996 0.0 0.0 0.0 0.0 86-87 0.4625 0.0 0.0 0.0 0.0 88-89 0.6125 0.0 0.0 0.0 0.0 90-91 0.7625 0.0 0.0 0.0 0.0 92-93 0.9 0.0 0.0 0.0 0.0 94-95 1.0375 0.0 0.0 0.0 0.0 96-97 1.175 0.0 0.0 0.0 0.0 98-99 1.475 0.0 0.0 0.0 0.0 100-101 1.75 0.0 0.0 0.0 0.0 102-103 2.0250000000000004 0.0 0.0 0.0 0.0 104-105 2.175 0.0 0.0 0.0 0.0 106-107 2.4 0.0 0.0 0.0 0.0 108-109 2.8 0.0 0.0 0.0 0.0 110-111 3.1125 0.0 0.0 0.0 0.0 112-113 3.3375 0.0 0.0 0.0 0.0 114-115 3.5875 0.0 0.0 0.0 0.0 116-117 4.05 0.0 0.0 0.0 0.0 118-119 4.625 0.0 0.0 0.0 0.0 120-121 5.2375 0.0 0.0 0.0 0.0 122-123 5.7875 0.0 0.0 0.0 0.0 124-125 6.4 0.0 0.0 0.0 0.0 126-127 7.35 0.0 0.0 0.0 0.0 128-129 8.075 0.0 0.0 0.0 0.0 130-131 9.0 0.0 0.0 0.0 0.0 132-133 9.5 0.0 0.0 0.0 0.0 134-135 10.1625 0.0 0.0 0.0 0.0 136-137 10.8 0.0 0.0 0.0 0.0 138-139 11.575 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618693 spots for SRR13695477.sra Written 618693 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra Read 618684 spots for SRR13695477.sra Written 618684 spots for SRR13695477.sra SRR ids: ['SRR13695477.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_aggfhktl SRR13695477.sra spots: 12373689 blocks: [[1, 618684], [618685, 1237368], [1237369, 1856052], [1856053, 2474736], [2474737, 3093420], [3093421, 3712104], [3712105, 4330788], [4330789, 4949472], [4949473, 5568156], [5568157, 6186840], [6186841, 6805524], [6805525, 7424208], [7424209, 8042892], [8042893, 8661576], [8661577, 9280260], [9280261, 9898944], [9898945, 10517628], [10517629, 11136312], [11136313, 11754996], [11754997, 12373689]] SRR13695477 file size 4183420 SRR13695477 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695477 SRR13695477_1.fastq SRR13695477_2.fastq Input file: SRR13695477_1.fastq Paired file: SRR13695477_2.fastq trimmed: SRR13695477-trimmed-pair1.fastq, SRR13695477-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 06:25:57 2025 >> started Wed Feb 12 06:26:10 2025 >> done (13.062s) 12373689 read pairs processed; of these: 96 ( 0.00%) short read pairs filtered out after trimming by size control 10727 ( 0.09%) empty read pairs filtered out after trimming by size control 12362866 (99.91%) read pairs available; of these: 2151819 (17.41%) trimmed read pairs available after processing 10211047 (82.59%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 5 0.00% 20 1 0.00% 21 2 0.00% 22 2 0.00% 23 2 0.00% 24 1 0.00% 25 3 0.00% 26 1 0.00% 27 5 0.00% 28 3 0.00% 29 2 0.00% 30 2 0.00% 31 2 0.00% 32 5 0.00% 33 4 0.00% 34 2 0.00% 35 4 0.00% 36 9 0.00% 37 11 0.00% 38 5 0.00% 39 8 0.00% 40 10 0.00% 41 14 0.00% 42 14 0.00% 43 9 0.00% 44 13 0.00% 45 18 0.00% 46 18 0.00% 47 21 0.00% 48 21 0.00% 49 29 0.00% 50 32 0.00% 51 44 0.00% 52 64 0.00% 53 61 0.00% 54 80 0.00% 55 89 0.00% 56 82 0.00% 57 89 0.00% 58 120 0.00% 59 136 0.00% 60 211 0.00% 61 252 0.00% 62 278 0.00% 63 332 0.00% 64 427 0.00% 65 451 0.00% 66 481 0.00% 67 593 0.00% 68 668 0.01% 69 820 0.01% 70 915 0.01% 71 1210 0.01% 72 1279 0.01% 73 1499 0.01% 74 1655 0.01% 75 1917 0.02% 76 2282 0.02% 77 2464 0.02% 78 2811 0.02% 79 3062 0.02% 80 3489 0.03% 81 3897 0.03% 82 4473 0.04% 83 4938 0.04% 84 5728 0.05% 85 6428 0.05% 86 6553 0.05% 87 7325 0.06% 88 7608 0.06% 89 8476 0.07% 90 8769 0.07% 91 9588 0.08% 92 10040 0.08% 93 11114 0.09% 94 12470 0.10% 95 12816 0.10% 96 14089 0.11% 97 14561 0.12% 98 15394 0.12% 99 16090 0.13% 100 16513 0.13% 101 17331 0.14% 102 18382 0.15% 103 19294 0.16% 104 19748 0.16% 105 21371 0.17% 106 22239 0.18% 107 23171 0.19% 108 24278 0.20% 109 24698 0.20% 110 25956 0.21% 111 26555 0.21% 112 27636 0.22% 113 28154 0.23% 114 29414 0.24% 115 30528 0.25% 116 31763 0.26% 117 33159 0.27% 118 33996 0.27% 119 33937 0.27% 120 34447 0.28% 121 35484 0.29% 122 36546 0.30% 123 36607 0.30% 124 37904 0.31% 125 38466 0.31% 126 40120 0.32% 127 41430 0.34% 128 41753 0.34% 129 42813 0.35% 130 43587 0.35% 131 43399 0.35% 132 44588 0.36% 133 44624 0.36% 134 46028 0.37% 135 46768 0.38% 136 46948 0.38% 137 48044 0.39% 138 48627 0.39% 139 50179 0.41% 140 50167 0.41% 141 51278 0.41% 142 52665 0.43% 143 52495 0.42% 144 52216 0.42% 145 53358 0.43% 146 53650 0.43% 147 55059 0.45% 148 55252 0.45% 149 54895 0.44% 150 55802 0.45% 151 10211047 82.59% 12362866 reads passed initial QC criterion=sequence-density sequence-density=0.57 sequence-density-rank=1 fanout-score=1.93 fanout-score-rank=34 prefix-density=0.57 prefix-fanout=1.9 sequence=GTGTTGTCGAATCC criterion=fanout-score sequence-density=0.02 sequence-density-rank=31 fanout-score=144.48 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=14.4 sequence=CTGCTGCTGCATTTATTAGAGAAGGAGATGCTGGTAATCTCCATTTCACAAGTTCATTAATCTCGATCGAAAATATGGCTCATTTAAGCATATACAAAGTACATCTGGGAAAGAAAGTTAAGAACCAAGATAGGGTCACTGATATTTGGATGATGTTCATACGGAGAGGGAGGGAGAAAGGCTGTACTCAAGCCCCCAGAGCTCAAAACTGCCTTTGACAAAATCATAATAACCACCCTTCAGTCCTAGAGTTTTGTTCACCAAGCCATCTCTCACAAACGGGTAGGTTAGCAAGTGTCCAAGGGACACGTTCACTGCCTCCTTTTCACATTGTGTACAGAGGTCTGGGAAAGGTGCATTGGCATGTTCTGCTAAAACCTTAGTCTTGGCAGGGTA criterion=sequence-density sequence-density=0.43 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=25 prefix-density=0.43 prefix-fanout=2.0 sequence=CTACCCATGTTTGGATGCACTGAGGCATCTCA criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=16.41 fanout-score-rank=1 prefix-density=0.19 prefix-fanout=1.0 sequence=GCTACACAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGA SRR13695477 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 06:27:20 Started mapping on | Feb 12 06:27:20 Finished on | Feb 12 06:30:32 Mapping speed, Million of reads per hour | 231.80 Number of input reads | 12362866 Average input read length | 285 UNIQUE READS: Uniquely mapped reads number | 8794351 Uniquely mapped reads % | 71.14% Average mapped length | 286.66 Number of splices: Total | 7984456 Number of splices: Annotated (sjdb) | 7820509 Number of splices: GT/AG | 7808683 Number of splices: GC/AG | 143565 Number of splices: AT/AC | 5523 Number of splices: Non-canonical | 26685 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 2.84 Insertion rate per base | 0.02% Insertion average length | 2.38 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 231747 % of reads mapped to multiple loci | 1.87% Number of reads mapped to too many loci | 70356 % of reads mapped to too many loci | 0.57% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 26.20% % of reads unmapped: other | 0.22% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3337041 3337041 3337041 N_multimapping 231747 231747 231747 N_noFeature 218965 8617864 276733 N_ambiguous 225671 1856 105866 UnstrandedReadsAssigned:8349715 PositiveStrandReadsAssigned:174631 NegativeStrandReadsAssigned:8411752 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695477 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695477-trimmed-pair1.fastq SRR13695477-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 12,362,866 reads, 9,999,377 reads pseudoaligned [quant] estimated average fragment length: 211.916 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,315 rounds 52401 SRR13695477.ke.tsv 34699 SRR13695477.se.tsv 87100 total ==> SRR13695477.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1807.08 332 15.0157 Potri.005G024800.1.v4.1 1035 824.084 225 22.315 Potri.004G059700.1.v4.1 961 750.093 5 0.544804 Potri.007G009000.2.v4.1 1416 1205.08 0 0 Potri.003G141000.2.v4.1 2943 2732.08 440 13.1627 Potri.016G087400.1.v4.1 270 96.8122 677 571.537 Potri.015G069301.1.v4.1 564 355.956 0 0 Potri.010G195200.1.v4.1 1773 1562.08 67 3.50555 Potri.012G127500.1.v4.1 977 766.093 59 6.29442 ==> SRR13695477.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 118 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 327 Potri.001G212900.v4.1 4 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 2 SRR13695477 completed mapping pipeline successfully