Starting /dee2/code/volunteer_pipeline.sh SRR13695478 current disk space = 3049679454208 free memory = 842650064 SRR13695478 SRAfilesize 5a3507a800226fe60ec4dc84e0eab11d SRR13695478.sra SRR13695478.sra file validated SRR13695478 is paired end SRR13695478 is conventional basespace SRR13695478 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695478_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5235 37.0 37.0 37.0 37.0 37.0 2 36.32275 37.0 37.0 37.0 37.0 37.0 3 36.582 37.0 37.0 37.0 37.0 37.0 4 36.6405 37.0 37.0 37.0 37.0 37.0 5 36.644 37.0 37.0 37.0 37.0 37.0 6 36.5795 37.0 37.0 37.0 37.0 37.0 7 36.5345 37.0 37.0 37.0 37.0 37.0 8 36.544 37.0 37.0 37.0 37.0 37.0 9 36.573 37.0 37.0 37.0 37.0 37.0 10-14 36.58149999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.5345 37.0 37.0 37.0 37.0 37.0 20-24 36.5358 37.0 37.0 37.0 37.0 37.0 25-29 36.456900000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.433899999999994 37.0 37.0 37.0 37.0 37.0 35-39 36.3775 37.0 37.0 37.0 37.0 37.0 40-44 36.3808 37.0 37.0 37.0 37.0 37.0 45-49 36.3803 37.0 37.0 37.0 37.0 37.0 50-54 36.364900000000006 37.0 37.0 37.0 37.0 37.0 55-59 36.3643 37.0 37.0 37.0 37.0 37.0 60-64 36.3877 37.0 37.0 37.0 37.0 37.0 65-69 36.342800000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.3332 37.0 37.0 37.0 37.0 37.0 75-79 36.2779 37.0 37.0 37.0 37.0 37.0 80-84 36.1735 37.0 37.0 37.0 37.0 37.0 85-89 36.21040000000001 37.0 37.0 37.0 37.0 37.0 90-94 36.1476 37.0 37.0 37.0 37.0 37.0 95-99 36.1009 37.0 37.0 37.0 37.0 37.0 100-104 36.1223 37.0 37.0 37.0 37.0 37.0 105-109 36.133 37.0 37.0 37.0 37.0 37.0 110-114 36.0949 37.0 37.0 37.0 37.0 37.0 115-119 36.053399999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.9934 37.0 37.0 37.0 37.0 37.0 125-129 35.941 37.0 37.0 37.0 37.0 37.0 130-134 35.949400000000004 37.0 37.0 37.0 37.0 37.0 135-139 35.891 37.0 37.0 37.0 37.0 37.0 140-144 35.7977 37.0 37.0 37.0 37.0 37.0 145-149 35.5556 37.0 37.0 37.0 37.0 37.0 150-151 35.35925 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 1.0 22 1.0 23 1.0 24 2.0 25 2.0 26 5.0 27 7.0 28 14.0 29 21.0 30 34.0 31 38.0 32 47.0 33 69.0 34 126.0 35 307.0 36 2957.0 37 367.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.900000000000006 12.025 8.425 45.65 2 18.538055764883197 14.694800301431801 38.33207736749561 28.4350665661894 3 17.675 15.625 26.55 40.150000000000006 4 20.5 23.150000000000002 25.1 31.25 5 23.400000000000002 31.0 23.425 22.175 6 20.349999999999998 34.825 23.9 20.925 7 15.375 27.3 41.6 15.725 8 18.224999999999998 25.525 33.1 23.150000000000002 9 16.3 23.375 35.925000000000004 24.4 10-14 19.165 29.685 27.27 23.880000000000003 15-19 20.075000000000003 28.155 27.985 23.785 20-24 19.925 27.805000000000003 28.449999999999996 23.82 25-29 20.18 27.025 29.509999999999998 23.285 30-34 19.98 28.689999999999998 26.884999999999998 24.445 35-39 19.905 28.725 27.744999999999997 23.625 40-44 19.96 27.855 27.700000000000003 24.485 45-49 20.380000000000003 28.07 27.455000000000002 24.095 50-54 20.549999999999997 27.725 27.63 24.095 55-59 20.585 28.139999999999997 28.050000000000004 23.225 60-64 20.36 28.384999999999998 27.98 23.275000000000002 65-69 20.580000000000002 27.85 27.785 23.785 70-74 20.05 28.525 27.794999999999998 23.630000000000003 75-79 19.919999999999998 28.044999999999998 27.744999999999997 24.29 80-84 20.325 28.389999999999997 27.27 24.015 85-89 20.39 28.754999999999995 27.74 23.115 90-94 20.765 28.415000000000003 27.375 23.445 95-99 20.875 28.765 27.04 23.32 100-104 21.055 28.595 26.985 23.365 105-109 20.77 27.985 27.700000000000003 23.544999999999998 110-114 20.355 28.325 26.985 24.335 115-119 21.36 28.235 27.18 23.225 120-124 20.84 27.415 27.355 24.39 125-129 20.185 28.105000000000004 27.13 24.58 130-134 20.93 28.1 27.01 23.96 135-139 20.655 28.060000000000002 27.3 23.985 140-144 21.595 27.560000000000002 26.83 24.015 145-149 20.745 27.750000000000004 27.389999999999997 24.115000000000002 150-151 21.175 28.075 26.474999999999998 24.275 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 1.5 24 2.5 25 5.0 26 7.0 27 7.0 28 9.0 29 12.0 30 17.5 31 18.0 32 23.0 33 48.0 34 62.5 35 79.0 36 92.5 37 111.0 38 132.5 39 147.0 40 177.5 41 200.5 42 217.5 43 247.5 44 267.0 45 282.5 46 266.5 47 232.5 48 226.0 49 204.0 50 177.0 51 150.5 52 121.0 53 83.0 54 70.0 55 85.5 56 75.0 57 47.5 58 28.0 59 15.5 60 17.5 61 14.5 62 5.5 63 4.5 64 1.5 65 0.0 66 0.5 67 1.0 68 0.5 69 0.0 70 0.0 71 0.5 72 0.5 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.475 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.65 #Duplication Level Percentage of deduplicated Percentage of total 1 70.4730229120473 47.675 2 19.142645971914266 25.900000000000002 3 5.838876570583888 11.85 4 2.5868440502586845 7.000000000000001 5 1.2195121951219512 4.125 6 0.3325942350332594 1.35 7 0.18477457501847747 0.8750000000000001 8 0.18477457501847747 1.0 9 0.03695491500369549 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTAGTTCCACCAAGTCTACCAGCTCCGGCATCAAGATCGTTGATGAAGA 9 0.22499999999999998 No Hit CCCAGAAGCATCAAGCCCACCCCTTAAATCCATGCCACCATTGATTCCAT 8 0.2 No Hit CTCCTTGACCTCCTTCAGAGCCTTCATTGTGCCGTGGAGCGCATGTCTGT 8 0.2 No Hit GGCAAAGACGCTTGGTCCCATCAATACACATGGCTTCACAACGACCATTT 8 0.2 No Hit CCTCTATCAATGGAATCTTGATTGGCTTCACCAAGAGAAGCCTCGCTCAG 8 0.2 No Hit TGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTT 8 0.2 No Hit CTTCAATCTTCCTTCTTCCTCTACATAAATTAACACTCTTCGCTTTTCTT 7 0.17500000000000002 No Hit CAGGGAATTCACCGGTCAGGTATGATGGGGTTTGCTCAGAGAATGGACCC 7 0.17500000000000002 No Hit GCCACCCTCGCTGAAGATCTGGGCGCCAGCCTTGAACCACACAGCCTCAC 7 0.17500000000000002 No Hit GTCACAAGAAGGGTACGGTATGCCTGGCGGTTAGCCTCGGTGTTCTCTAG 7 0.17500000000000002 No Hit TTCCAATGTAGTATGGTGGCCACATCCTTTACCAAGATAGGGTCACTGAT 7 0.17500000000000002 No Hit CTCCGGTTCCATTCCCCGCAGTAAAATCCCCTCCTTGACACATGAATCCA 6 0.15 No Hit GTGAGCTAGGATCCATGCATCCTGAGACTAGATCAAGATCGCAACGATCA 6 0.15 No Hit TGGAGATGACAAATTAGAGGATGCACCACAGTTAAGGAGTATTAGATCAG 6 0.15 No Hit CCACCTCATCATTAACAGTCTTTAGTTCTGACACCGCAGCTGAGTACCTG 6 0.15 No Hit GTCCCATTTCAGTACCAAAGTGGAGGAAGGTACTCCATATGCTCGTCATA 6 0.15 No Hit GAGAGAGATAGGAGTTGGGGTTTGGTGATCTTGAGGATTTTAGGGAAGGA 6 0.15 No Hit GTACAGCCATGACATTCTTACGGAATGATCTAATGGGTGGCCAACCCACA 6 0.15 No Hit ATCGGATCCGGCAGAGTCTTTGTCTGAAGGGAGATTGATAGATTGCTTTG 6 0.15 No Hit GGCAGAAAAGCAGACATAAACTAGTCTAAACTGTAACTAAGCAAACACTT 6 0.15 No Hit GCCAAACTTCTGGTCTAGGACCTTGACGAAGGGAGCAAGGCAGTTAGTGG 5 0.125 No Hit CTTTTCTCTCTTCCTCCGTCAAATTACGATCTGCACCCATGAGTTCATCT 5 0.125 No Hit GATCAGTTTATGTTATTTGGTTATGTTCTTTGACCATTATGGTCAGCAGA 5 0.125 No Hit CCTCCCATTACCAAGCTGCGTAAGTACTGAGCATTTGCAGCCCCTGTACT 5 0.125 No Hit CTCCCGAGTTGCTATGCTTTCAAGATGAAAGAGTAAACTAAGTAAAAAAG 5 0.125 No Hit CCCTTAATGACAACAGAAGTGACTCCTTTATCGACGCAGAAATAAGTTCC 5 0.125 No Hit CACTCATCCAAGAGCTCATCAAATCTTCACAATCAAGCTGCAACGACAGG 5 0.125 No Hit GCCTTTTTGAGTTCATCAATGTTGCATCAGGTTTATTAGCAGAAGTTGGC 5 0.125 No Hit GCATTTTTCTCAAATCCTCTCCTTGTGCCTTGATAAATTTGTCATAAGTT 5 0.125 No Hit GCCTGATATTTTTCGAAGTCGTCTGGCTTCTTGCTGAGACCAAAAGGATC 5 0.125 No Hit TACACGAAGAGCAAACTGATCCACCCATCTTGGTGGAGCTCACATAGACG 5 0.125 No Hit CCGGATTTTTGAATCAGGGGTAGTGACTTAACAGCTCGCTCAATCTTGGT 5 0.125 No Hit CCATCCTTAACTTCTTCTCCAAATCTCTTTTTCCACATTGAATGAGCCCG 5 0.125 No Hit CACTAGATAATGGACCCAAATCAAACTACTCTTTCATTTTCAAGGGCGAT 5 0.125 No Hit CAGAGGTCTGGGAAAGGTGCATTGGCATGTTCTGCTAAAACCTTAGTCTT 5 0.125 No Hit TGCTGCATCACCCACTAACGCTACCCTCCCTGATAATCTGCGAGGCCGGG 5 0.125 No Hit CTACCATTCTTGAGTTCCTTCACCTTCAACTCAGCGAATGCTTCGGGATC 5 0.125 No Hit CCGCAGTTTGTCGAAGGAAGATAATGTTGTTGTGGTTGGTGTCGGCAGAT 5 0.125 No Hit GGGTAAATCAAAGATGTGGTTGGTGATCCACTAGGATTCACGCATCAACA 5 0.125 No Hit CAAGAAAGTAATCTCGTGTAAGTATGGCATAGAAAGTCCAGATCCCTCCA 5 0.125 No Hit CGCTGAGGGTGTCAGCCAGAAATCTTAGCAATAATTCGCCACAATTGGAC 5 0.125 No Hit CACATGTGTCTTGGTCAAGATGTTCACAAGTTATTTCTCCTAGAGTACCT 5 0.125 No Hit GACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTG 5 0.125 No Hit CAATAGTCCCAGCACAGAAAAAACTCTTTTGTTGCTCACTTTCCGGGAAC 5 0.125 No Hit TAGTTCACCACCACTAGTTATAACACATGTGTGATAGCCACCACATGCTA 5 0.125 No Hit GTTGTAAAGAAGGAGCCAGCATACTTGCCTTGAGGGGCTTGGTTATGACA 5 0.125 No Hit GTAGTCATCATTGTCAAAATCATAACCAATGGCCTTGAGACCTGCCATGG 5 0.125 No Hit CTTGAGGGCATCAAGGTAATCCAAATCAGAGTTTCGATGCACAAAGCGGC 5 0.125 No Hit GTTCCACAAAGTGTGCAAGCCGAGTTTGAGCACTCAGAAATCTTTGGATG 5 0.125 No Hit GTCACTTGATGCACTCGTGTTACTTCCTCATTGCTTCCTAATTGTAGATT 5 0.125 No Hit CCCACATTTCTTTCGACGAGAAGGGGTTCTCCATTTTCGTTAGTTATATA 5 0.125 No Hit TTTCCATAGTCGACCAATTCTCGAGCTGCCATGGATAAAGGAGAGAAGGC 5 0.125 No Hit TGTGTCTTCAAGTTGTCTTAGACTTCTGTGGCGATGGAAGCAACTGGTAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.1125 0.0 0.0 0.0 0.0 86-87 0.3375 0.0 0.0 0.0 0.0 88-89 0.4625 0.0 0.0 0.0 0.0 90-91 0.525 0.0 0.0 0.0 0.0 92-93 0.5874999999999999 0.0 0.0 0.0 0.0 94-95 0.6625 0.0 0.0 0.0 0.0 96-97 0.9375 0.0 0.0 0.0 0.0 98-99 1.1125 0.0 0.0 0.0 0.0 100-101 1.2375 0.0 0.0 0.0 0.0 102-103 1.4 0.0 0.0 0.0 0.0 104-105 1.55 0.0 0.0 0.0 0.0 106-107 1.8 0.0 0.0 0.0 0.0 108-109 1.9625 0.0 0.0 0.0 0.0 110-111 2.125 0.0 0.0 0.0 0.0 112-113 2.4000000000000004 0.0 0.0 0.0 0.0 114-115 2.825 0.0 0.0 0.0 0.0 116-117 3.2 0.0 0.0 0.0 0.0 118-119 3.7249999999999996 0.0 0.0 0.0 0.0 120-121 3.925 0.0 0.0 0.0 0.0 122-123 4.175 0.0 0.0 0.0 0.0 124-125 4.525 0.0 0.0 0.0 0.0 126-127 4.987500000000001 0.0 0.0 0.0 0.0 128-129 5.4625 0.0 0.0 0.0 0.0 130-131 6.0375 0.0 0.0 0.0 0.0 132-133 6.449999999999999 0.0 0.0 0.0 0.0 134-135 6.825 0.0 0.0 0.0 0.0 136-137 7.3125 0.0 0.0 0.0 0.0 138-139 7.9875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATGTA 10 0.006830828 145.0 4 CCAATGT 10 0.006830828 145.0 3 TAGTATG 10 0.006830828 145.0 9 TGTAGTA 10 0.006830828 145.0 7 ATGTAGT 10 0.006830828 145.0 6 GTAGTAT 10 0.006830828 145.0 8 GTTCGCA 10 0.006830828 145.0 1 GATCGGA 40 0.0076550315 18.125 140-144 >>END_MODULE SRR13695478 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695478_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1905 37.0 37.0 37.0 37.0 37.0 2 36.1725 37.0 37.0 37.0 37.0 37.0 3 36.2435 37.0 37.0 37.0 37.0 37.0 4 36.2105 37.0 37.0 37.0 37.0 37.0 5 36.3705 37.0 37.0 37.0 37.0 37.0 6 36.3035 37.0 37.0 37.0 37.0 37.0 7 36.2875 37.0 37.0 37.0 37.0 37.0 8 36.342 37.0 37.0 37.0 37.0 37.0 9 36.288 37.0 37.0 37.0 37.0 37.0 10-14 36.329899999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.3056 37.0 37.0 37.0 37.0 37.0 20-24 36.25695 37.0 37.0 37.0 37.0 37.0 25-29 36.22265 37.0 37.0 37.0 37.0 37.0 30-34 36.18705 37.0 37.0 37.0 37.0 37.0 35-39 36.141650000000006 37.0 37.0 37.0 37.0 37.0 40-44 36.16674999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.119099999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.13155 37.0 37.0 37.0 37.0 37.0 55-59 36.09855 37.0 37.0 37.0 37.0 37.0 60-64 36.076049999999995 37.0 37.0 37.0 37.0 37.0 65-69 35.96455 37.0 37.0 37.0 37.0 37.0 70-74 35.98535 37.0 37.0 37.0 37.0 37.0 75-79 35.933350000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.95179999999999 37.0 37.0 37.0 37.0 37.0 85-89 35.8862 37.0 37.0 37.0 37.0 37.0 90-94 35.91275 37.0 37.0 37.0 37.0 37.0 95-99 35.8847 37.0 37.0 37.0 37.0 37.0 100-104 35.859249999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.79685 37.0 37.0 37.0 37.0 37.0 110-114 35.787349999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.816050000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.61785 37.0 37.0 37.0 37.0 37.0 125-129 35.745999999999995 37.0 37.0 37.0 37.0 37.0 130-134 35.57215 37.0 37.0 37.0 37.0 37.0 135-139 35.53985 37.0 37.0 37.0 37.0 37.0 140-144 35.45865 37.0 37.0 37.0 37.0 37.0 145-149 35.287150000000004 37.0 37.0 37.0 34.6 37.0 150-151 35.016999999999996 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 2.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 1.0 14 1.0 15 2.0 16 2.0 17 1.0 18 0.0 19 0.0 20 1.0 21 1.0 22 1.0 23 3.0 24 4.0 25 2.0 26 11.0 27 11.0 28 9.0 29 22.0 30 24.0 31 29.0 32 56.0 33 104.0 34 191.0 35 539.0 36 2755.0 37 225.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.12293025589563 21.726041144004014 12.117410938283994 31.033617661816358 2 28.275 22.900000000000002 33.550000000000004 15.275 3 21.125 27.400000000000002 31.35 20.125 4 22.900000000000002 33.800000000000004 24.025 19.275000000000002 5 24.6 36.0 23.974999999999998 15.425 6 20.674999999999997 38.4 23.3 17.625 7 21.0 22.225 37.875 18.9 8 21.3 27.224999999999998 27.750000000000004 23.724999999999998 9 22.575 24.7 30.25 22.475 10-14 22.585 29.825000000000003 26.855 20.735 15-19 21.81 28.93 28.244999999999997 21.015 20-24 22.959107062415537 28.805245507783173 27.64903148305721 20.58661594674408 25-29 23.033792240300375 27.674593241551943 28.420525657071337 20.871088861076345 30-34 22.010311858637433 27.726885918806627 28.708014216348797 21.554788006207136 35-39 22.919065018269183 27.809199659642626 27.79418389308774 21.47755142900045 40-44 22.636031436151573 28.622916353806875 28.07228312559443 20.668769084447113 45-49 22.970267294023426 28.236059665632197 28.196015617178897 20.597657423165483 50-54 22.05816106912258 27.754141848941387 28.8653085740027 21.32238850793333 55-59 23.15894868585732 28.260325406758447 27.88485607008761 20.69586983729662 60-64 22.899043996196006 27.83923119275239 28.10450973522198 21.157215075829622 65-69 24.0180135101326 27.655741806354765 27.500625469101823 20.82561921441081 70-74 23.78473091364205 27.95994993742178 27.28911138923655 20.966207759699625 75-79 23.28677979676628 27.801972268108326 28.22746158081794 20.683786354307454 80-84 23.55855855855856 27.982982982982985 27.307307307307305 21.15115115115115 85-89 23.928714457348818 28.188826591910292 27.452943532238688 20.429515418502202 90-94 23.04419640622654 27.288653085740027 27.814204915160918 21.852945592872516 95-99 23.27827827827828 28.74874874874875 26.95195195195195 21.02102102102102 100-104 23.69461827284105 28.235294117647058 27.2090112640801 20.86107634543179 105-109 23.484355444305383 28.090112640801003 27.76971214017522 20.6558197747184 110-114 23.704890134641374 28.01441513589269 27.30366885229491 20.97702587717103 115-119 24.901126408010015 28.50563204005006 26.16770963704631 20.425531914893615 120-124 24.921151439299123 28.630788485607006 26.663329161451816 19.78473091364205 125-129 25.015018021625952 28.16880256307569 26.962354825790953 19.85382458950741 130-134 24.520650813516895 28.195244055068834 27.614518147684606 19.669586983729662 135-139 25.742177722152693 27.44430538172716 27.614518147684606 19.198998748435546 140-144 25.87717102958106 27.914310025526802 26.177486360678714 20.031032584213424 145-149 26.74843554443054 26.923654568210264 26.708385481852314 19.619524405506883 150-151 26.583229036295368 29.161451814768462 25.619524405506883 18.635794743429287 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 1.0 8 1.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.5 15 0.5 16 0.5 17 0.5 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 1.0 24 4.5 25 5.0 26 6.5 27 10.0 28 7.5 29 15.5 30 19.5 31 21.5 32 22.5 33 32.5 34 56.0 35 65.0 36 90.0 37 118.0 38 135.5 39 158.5 40 188.0 41 236.5 42 290.5 43 285.0 44 261.0 45 268.5 46 270.0 47 232.0 48 196.0 49 181.0 50 172.5 51 146.0 52 107.0 53 91.0 54 68.5 55 60.5 56 52.0 57 30.5 58 23.0 59 26.0 60 17.5 61 7.5 62 4.5 63 1.0 64 0.0 65 1.0 66 1.0 67 0.5 68 0.5 69 0.0 70 0.0 71 0.5 72 0.5 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.5 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.105 25-29 0.125 30-34 0.11499999999999999 35-39 0.105 40-44 0.11499999999999999 45-49 0.11 50-54 0.105 55-59 0.125 60-64 0.105 65-69 0.075 70-74 0.125 75-79 0.11499999999999999 80-84 0.1 85-89 0.12 90-94 0.105 95-99 0.1 100-104 0.125 105-109 0.125 110-114 0.105 115-119 0.125 120-124 0.125 125-129 0.12 130-134 0.125 135-139 0.125 140-144 0.105 145-149 0.125 150-151 0.125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.95 #Duplication Level Percentage of deduplicated Percentage of total 1 71.26563649742458 48.425000000000004 2 18.395879323031643 25.0 3 6.070640176600441 12.375 4 2.2442972774098604 6.1 5 1.1037527593818985 3.75 6 0.5518763796909493 2.25 7 0.14716703458425312 0.7000000000000001 8 0.18395879323031641 1.0 9 0.0 0.0 >10 0.03679175864606328 0.4 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 16 0.4 No Hit CACTGATTGATTTTCTTTTTCTATCTATCTATCTATCAGCTTCCTCGCTC 8 0.2 No Hit CAGCTCAGTTATGGCTTCATCAATGAGCCTGAAACCAGCTCCTTTTACAG 8 0.2 No Hit CCACGAGTCACCGTCAAAATGGCCAAGTCCAAGAACTCGTCTCAGCACAA 8 0.2 No Hit AAGAACTTCATGAGCCTGCCTAATATCAAGGTTCCTCTCATCTTGGGTGT 8 0.2 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 8 0.2 No Hit AGCTTGTCAAAACCGCGAAAACAATTGCATCTCCTGGCCGAGGTATTTTG 7 0.17500000000000002 No Hit ACAAAAACAACAACAAGAGAGAGAAGAACTGGAAACAGGAAACAAGAAAC 7 0.17500000000000002 No Hit GAAAAGCAGAGCAAGCAACTTAGAGGCAGCATTAACAAAGAAGAGTCATG 7 0.17500000000000002 No Hit ACGGTCCAGACCGTGTTAAATACCTGGGTCCTTTCTCCGGTGAGCCCCCA 7 0.17500000000000002 No Hit GGACACCACAACTCTAAATTTTGCATCTTTAGTCATCAGCCATGGCCAAC 6 0.15 No Hit GTTAGGCTTGAGCAGATTCATTCGCCAACTAACCCTTTAATTTATCCTAT 6 0.15 No Hit AGAGACGCAAACTTGTAGACCAAGAACTAGAGACGGTACAGGTGGAGATG 6 0.15 No Hit GTTACCTGGAGGAATTGGTAACGGAAATGATGGTGAGGTTGCAAAGAGTA 6 0.15 No Hit GGCTGCAGTTTCAAGTTCACAATGGCTGGCGAAACAAGCCCATCGAATTC 6 0.15 No Hit GATTGATAACCTTGACTAAACATATGGAACTTTGTGGACTCTTGTCAGTG 6 0.15 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 6 0.15 No Hit GTGGAGGGAAGAGTTTTGGACGACGAAAACCTCTTCAAAGCAATCTATCA 6 0.15 No Hit GGCGATCTGGCAAAGAGCTCGAGAAGGAGCCCAAGTTCTTGAAGAATGGT 6 0.15 No Hit GTCAAAGTTTGCTACCCTGCCAAGACTAAGGTTTTAGCAGAACATGCCAA 6 0.15 No Hit TGGCAAACCCTAAGGTCTACTTCGACATGACAATCGGCGGCGTCCCAGCC 6 0.15 No Hit CTTGCATGCTCTTGGAGCCTAATCGGATTGGCCCAGACAGGGTGCAAGCA 6 0.15 No Hit TTTATGACGATGAGGTGAGGAAGTGGGTCTCAGGCGTTGGTGTTGACAGC 6 0.15 No Hit CGAAAGCCATTCTCTGAAAGAACATCAATATGGCTCCTAAACTTTCCTGT 6 0.15 No Hit ATAATGTCCATCTTCATTGCTCTCTACCTTCTCCCTCCCCTCCATCTCTT 6 0.15 No Hit GCAAGGTTTGAAACCAAAATATGACTGTCTTCTCTTCGATGTTGATGATA 5 0.125 No Hit CAGCTTTTATATCCCCTATTCTCTTCGTGAAGATGTCTTGCTGTGGAGGA 5 0.125 No Hit CTCTAGTTGCCGGTAAAAAGGTCATCCTCTTTGGTGTTCCCGGTGCCTTC 5 0.125 No Hit CTGACAGAAGAATCTTCTTGCCGGATGGGCTCTTGGACCGGTCTGAGATC 5 0.125 No Hit GTTGGCTATGTTCTCAATGTTCGGATTCTTTGTCCAGGCCATTGTGACCG 5 0.125 No Hit GTTAAAAACATTGCTGCCGGCTTGCTTCATTCAGCCTGCATTGATGAGAA 5 0.125 No Hit TGCTTATTATGGAAGAATTGGGTGGCAATTCTTTGTGGTTTGACCGCTTT 5 0.125 No Hit CGTCAATGAAGAAGTTGGAGAAGGAGCTTAAGGAGGGTGAAAAAAATCAT 5 0.125 No Hit GTTTTTCAAGTGTAATTATTGAGTTGTTTTTAGTTATTCAGAAAAAAAAA 5 0.125 No Hit CAGATTTCTACGGGTGGGTCTTCCCCAAATGTGACCATGTTGCTGTTGGA 5 0.125 No Hit AGCGTGTTTGCATATTTCAGCCATACATATCAAGTTGAGGAAACAATGTC 5 0.125 No Hit AGGATTCGAGCATTTCAGCTCTCTTTGTCAACAAGTTACAAAAAATTGTC 5 0.125 No Hit AGGAGAGGCATTGGCCATGTAAAACACAATATTTTTCAGTATGAAAGCTT 5 0.125 No Hit CTGGAATCAGAGAGAGCAAGGAATGGAAAAAACCTGGAAACAACGATGCC 5 0.125 No Hit GTGAAAGACGAGGGAGATAGTTTTGAGGATCTGCAGAAGATGTTTGTGGA 5 0.125 No Hit ATTACGGCAGGGGCCAAGAAGGTGATCATCACAGCCCCTGGAAAGGGTGA 5 0.125 No Hit AATACATTGTGGTCATTGGACACAGCGCCTGTGGTGGAATTAAGGGCCTC 5 0.125 No Hit TTTTCAATGCCACAATCGGTGAAGGTAATGAAGAGGAGTTCTCTATGGAA 5 0.125 No Hit GGTGAGGAGAGTAATTAAAAAAAAAAAGAGGGAAATAGGAGGCGTAAGAA 5 0.125 No Hit GTGGAAAGCAGAGTTCTATCGACTCTATAATTTGGAGTCAATTGAGGACT 5 0.125 No Hit CTTTTCTCTCTTCCTATCCAAATTCATAACCAAACCCTAAAAAGTAACAA 5 0.125 No Hit AGTAGCTGTCCTTTGCATAACGAAGTCCGGGGAAGTTCTAAACTACCAGG 5 0.125 No Hit GGAAGGAGTTTACACCACCAGCACTTGTGGCAGTACTCCCATGGATGTGA 5 0.125 No Hit GCCCTTTCACTGCAACAGAAGAGGAAAAGACTCAAGGGAGAGAAATGGAG 5 0.125 No Hit TGCCTATCAAAGTTTCTCTGCTCTGAATTCCGAGGCGGGATCTGCTCAAG 5 0.125 No Hit CGAGAAGAATTCAGATCATGTACAATGCTAATTATTGCACATCGACTCAA 5 0.125 No Hit TTTATTCCTTATTTACGCACCAGTAAAGATGAGGAATAAGACTGCCATTC 5 0.125 No Hit CTCTGTTATTAACGGATTTTAATTTTCTGTTTGAGAAAATTGTATATTAT 5 0.125 No Hit TTATGGATTGTGATTATAGTCTTTGAATCTAATTTGAATTGCTGCATTGT 5 0.125 No Hit CTCCTATTTTGTGCTTGAGTGATTCTCATAGACAGTTTTCTGTTGATAGA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.1125 0.0 0.0 0.0 0.0 86-87 0.3375 0.0 0.0 0.0 0.0 88-89 0.4625 0.0 0.0 0.0 0.0 90-91 0.525 0.0 0.0 0.0 0.0 92-93 0.5874999999999999 0.0 0.0 0.0 0.0 94-95 0.6625 0.0 0.0 0.0 0.0 96-97 0.9375 0.0 0.0 0.0 0.0 98-99 1.1125 0.0 0.0 0.0 0.0 100-101 1.2375 0.0 0.0 0.0 0.0 102-103 1.4 0.0 0.0 0.0 0.0 104-105 1.55 0.0 0.0 0.0 0.0 106-107 1.8 0.0 0.0 0.0 0.0 108-109 1.9625 0.0 0.0 0.0 0.0 110-111 2.125 0.0 0.0 0.0 0.0 112-113 2.3625 0.0 0.0 0.0 0.0 114-115 2.775 0.0 0.0 0.0 0.0 116-117 3.125 0.0 0.0 0.0 0.0 118-119 3.6500000000000004 0.0 0.0 0.0 0.0 120-121 3.8499999999999996 0.0 0.0 0.0 0.0 122-123 4.1125 0.0 0.0 0.0 0.0 124-125 4.475 0.0 0.0 0.0 0.0 126-127 4.9125 0.0 0.0 0.0 0.0 128-129 5.387499999999999 0.0 0.0 0.0 0.0 130-131 5.9625 0.0 0.0 0.0 0.0 132-133 6.3875 0.0 0.0 0.0 0.0 134-135 6.775 0.0 0.0 0.0 0.0 136-137 7.275 0.0 0.0 0.0 0.0 138-139 7.9625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGCTA 10 0.006830828 145.0 8 AAGTTTG 10 0.006830828 145.0 5 GATCGGA 40 0.0076550315 18.125 140-144 >>END_MODULE Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962563 spots for SRR13695478.sra Written 962563 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra Read 962553 spots for SRR13695478.sra Written 962553 spots for SRR13695478.sra SRR ids: ['SRR13695478.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_9b83am8z SRR13695478.sra spots: 19251070 blocks: [[1, 962553], [962554, 1925106], [1925107, 2887659], [2887660, 3850212], [3850213, 4812765], [4812766, 5775318], [5775319, 6737871], [6737872, 7700424], [7700425, 8662977], [8662978, 9625530], [9625531, 10588083], [10588084, 11550636], [11550637, 12513189], [12513190, 13475742], [13475743, 14438295], [14438296, 15400848], [15400849, 16363401], [16363402, 17325954], [17325955, 18288507], [18288508, 19251070]] SRR13695478 file size 6520655 SRR13695478 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695478 SRR13695478_1.fastq SRR13695478_2.fastq Input file: SRR13695478_1.fastq Paired file: SRR13695478_2.fastq trimmed: SRR13695478-trimmed-pair1.fastq, SRR13695478-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 05:39:07 2025 >> started Wed Feb 12 05:39:29 2025 >> done (21.743s) 19251070 read pairs processed; of these: 144 ( 0.00%) short read pairs filtered out after trimming by size control 1284 ( 0.01%) empty read pairs filtered out after trimming by size control 19249642 (99.99%) read pairs available; of these: 2174178 (11.29%) trimmed read pairs available after processing 17075464 (88.71%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 1 0.00% 20 2 0.00% 21 1 0.00% 22 0 0.00% 23 3 0.00% 24 1 0.00% 25 5 0.00% 26 2 0.00% 27 0 0.00% 28 1 0.00% 29 1 0.00% 30 1 0.00% 31 5 0.00% 32 5 0.00% 33 2 0.00% 34 3 0.00% 35 6 0.00% 36 6 0.00% 37 3 0.00% 38 18 0.00% 39 12 0.00% 40 17 0.00% 41 20 0.00% 42 27 0.00% 43 25 0.00% 44 16 0.00% 45 24 0.00% 46 28 0.00% 47 40 0.00% 48 25 0.00% 49 58 0.00% 50 88 0.00% 51 68 0.00% 52 118 0.00% 53 66 0.00% 54 115 0.00% 55 89 0.00% 56 130 0.00% 57 146 0.00% 58 167 0.00% 59 242 0.00% 60 308 0.00% 61 338 0.00% 62 339 0.00% 63 396 0.00% 64 418 0.00% 65 581 0.00% 66 571 0.00% 67 699 0.00% 68 772 0.00% 69 907 0.00% 70 1059 0.01% 71 1208 0.01% 72 1485 0.01% 73 1523 0.01% 74 1920 0.01% 75 2074 0.01% 76 2269 0.01% 77 2536 0.01% 78 2740 0.01% 79 2954 0.02% 80 3307 0.02% 81 3793 0.02% 82 4382 0.02% 83 4672 0.02% 84 5458 0.03% 85 5807 0.03% 86 6360 0.03% 87 6575 0.03% 88 7212 0.04% 89 7727 0.04% 90 8244 0.04% 91 9157 0.05% 92 9610 0.05% 93 10473 0.05% 94 11110 0.06% 95 12065 0.06% 96 12338 0.06% 97 13593 0.07% 98 14106 0.07% 99 14809 0.08% 100 15454 0.08% 101 15614 0.08% 102 16909 0.09% 103 17948 0.09% 104 18421 0.10% 105 19538 0.10% 106 20409 0.11% 107 21387 0.11% 108 22321 0.12% 109 23021 0.12% 110 23318 0.12% 111 24880 0.13% 112 25869 0.13% 113 26305 0.14% 114 27484 0.14% 115 28927 0.15% 116 29504 0.15% 117 31242 0.16% 118 31861 0.17% 119 32981 0.17% 120 33665 0.17% 121 34745 0.18% 122 35582 0.18% 123 36651 0.19% 124 37234 0.19% 125 38240 0.20% 126 40111 0.21% 127 40566 0.21% 128 41908 0.22% 129 42618 0.22% 130 44338 0.23% 131 44328 0.23% 132 44962 0.23% 133 46159 0.24% 134 46933 0.24% 135 47781 0.25% 136 48887 0.25% 137 50426 0.26% 138 51334 0.27% 139 53498 0.28% 140 53473 0.28% 141 54565 0.28% 142 55901 0.29% 143 55795 0.29% 144 57796 0.30% 145 58216 0.30% 146 58895 0.31% 147 60213 0.31% 148 62130 0.32% 149 62589 0.33% 150 63762 0.33% 151 17075464 88.71% 19249642 reads passed initial QC criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=25 prefix-density=0.41 prefix-fanout=2.0 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTT criterion=fanout-score sequence-density=0.02 sequence-density-rank=30 fanout-score=229.23 fanout-score-rank=1 prefix-density=0.26 prefix-fanout=15.2 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAG criterion=sequence-density sequence-density=1.14 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=31 prefix-density=1.13 prefix-fanout=2.0 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=61.61 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=4.6 sequence=CAACTTCAAGGGCAGTAGTCTTAAACCATACTCTAAAATCTTCTTATAATTCCAGTTGTAATATTCTGCTAGCATATAATGGCTTCTTCAATGAGCTTGAAGCTGGCCTGTGCCATGCTTGTAGCGATGGTTGTTAGTGCACCACTAGCAGAAGCTGCCATCTCATGTGGCCAGGTGTCAAGCAGCTTGGCACAATGTATAACCTACCTCCAGAAGGGTGGGGCTGTGCCTGCAGCTTGCTGCAGTGGGTTGAAAGGACTTAATTCTGCAGCCACGACCACCGCCGACCGCCAAGGGGTCTGCAACTGTTTGAAATCCTTGGCTGGTAAGATCTCTGGCATCAACTATGGCGTGGCTGCTGGCCTCCCTTCAAAGTGTGGTGTATCCATCTCCTACAAAATCAGTCCTTCCACAGATTGCAAAAGCGTGAAGTGAAGAAT SRR13695478 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 05:40:09 Started mapping on | Feb 12 05:40:09 Finished on | Feb 12 05:42:12 Mapping speed, Million of reads per hour | 563.40 Number of input reads | 19249642 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 18266165 Uniquely mapped reads % | 94.89% Average mapped length | 295.14 Number of splices: Total | 17492811 Number of splices: Annotated (sjdb) | 17099081 Number of splices: GT/AG | 17126626 Number of splices: GC/AG | 287263 Number of splices: AT/AC | 10754 Number of splices: Non-canonical | 68168 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.84 Insertion rate per base | 0.02% Insertion average length | 2.38 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 471595 % of reads mapped to multiple loci | 2.45% Number of reads mapped to too many loci | 50657 % of reads mapped to too many loci | 0.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.32% % of reads unmapped: other | 0.08% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 512091 512091 512091 N_multimapping 471595 471595 471595 N_noFeature 703893 17780859 976258 N_ambiguous 337056 1914 122748 UnstrandedReadsAssigned:17225216 PositiveStrandReadsAssigned:483392 NegativeStrandReadsAssigned:17167159 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695478 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695478-trimmed-pair1.fastq SRR13695478-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,249,642 reads, 17,094,630 reads pseudoaligned [quant] estimated average fragment length: 247.752 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,129 rounds 52401 SRR13695478.ke.tsv 34699 SRR13695478.se.tsv 87100 total ==> SRR13695478.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1771.25 488 13.0187 Potri.005G024800.1.v4.1 1035 788.248 212 12.7086 Potri.004G059700.1.v4.1 961 714.348 4 0.264592 Potri.007G009000.2.v4.1 1416 1169.25 0 0 Potri.003G141000.2.v4.1 2943 2696.25 1101 19.2954 Potri.016G087400.1.v4.1 270 84.239 948 531.766 Potri.015G069301.1.v4.1 564 326.152 0 0 Potri.010G195200.1.v4.1 1773 1526.25 141 4.36536 Potri.012G127500.1.v4.1 977 730.317 106 6.85836 ==> SRR13695478.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 233 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 331 Potri.001G212900.v4.1 4 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 7 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695478 completed mapping pipeline successfully