Starting /dee2/code/volunteer_pipeline.sh SRR13695479 current disk space = 3050331648000 free memory = 1436937440 SRR13695479 SRAfilesize 430941c1e70a94561bae2ee6df6d2a17 SRR13695479.sra SRR13695479.sra file validated SRR13695479 is paired end SRR13695479 is conventional basespace SRR13695479 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695479_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.599 37.0 37.0 37.0 37.0 37.0 2 36.20125 37.0 37.0 37.0 37.0 37.0 3 36.6055 37.0 37.0 37.0 37.0 37.0 4 36.579 37.0 37.0 37.0 37.0 37.0 5 36.584 37.0 37.0 37.0 37.0 37.0 6 36.6255 37.0 37.0 37.0 37.0 37.0 7 36.573 37.0 37.0 37.0 37.0 37.0 8 36.5365 37.0 37.0 37.0 37.0 37.0 9 36.5865 37.0 37.0 37.0 37.0 37.0 10-14 36.5655 37.0 37.0 37.0 37.0 37.0 15-19 36.562599999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.5029 37.0 37.0 37.0 37.0 37.0 25-29 36.470600000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.4511 37.0 37.0 37.0 37.0 37.0 35-39 36.472300000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.4606 37.0 37.0 37.0 37.0 37.0 45-49 36.4134 37.0 37.0 37.0 37.0 37.0 50-54 36.4199 37.0 37.0 37.0 37.0 37.0 55-59 36.416999999999994 37.0 37.0 37.0 37.0 37.0 60-64 36.4252 37.0 37.0 37.0 37.0 37.0 65-69 36.3078 37.0 37.0 37.0 37.0 37.0 70-74 36.3187 37.0 37.0 37.0 37.0 37.0 75-79 36.320100000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.2296 37.0 37.0 37.0 37.0 37.0 85-89 36.2863 37.0 37.0 37.0 37.0 37.0 90-94 36.1553 37.0 37.0 37.0 37.0 37.0 95-99 36.109500000000004 37.0 37.0 37.0 37.0 37.0 100-104 36.1898 37.0 37.0 37.0 37.0 37.0 105-109 36.17959999999999 37.0 37.0 37.0 37.0 37.0 110-114 36.110499999999995 37.0 37.0 37.0 37.0 37.0 115-119 36.090199999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.9809 37.0 37.0 37.0 37.0 37.0 125-129 36.009100000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.982800000000005 37.0 37.0 37.0 37.0 37.0 135-139 35.9029 37.0 37.0 37.0 37.0 37.0 140-144 35.7573 37.0 37.0 37.0 37.0 37.0 145-149 35.62480000000001 37.0 37.0 37.0 37.0 37.0 150-151 35.434 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 1.0 25 0.0 26 2.0 27 8.0 28 15.0 29 16.0 30 26.0 31 36.0 32 50.0 33 72.0 34 120.0 35 383.0 36 2878.0 37 392.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.725 10.65 8.450000000000001 41.175 2 21.545431663730177 12.685627988925246 35.43921469921973 30.329725648124846 3 20.05 17.375 25.874999999999996 36.7 4 23.05 26.05 21.875 29.025000000000002 5 24.875 31.35 24.125 19.650000000000002 6 20.325 35.55 23.5 20.625 7 16.400000000000002 27.275 39.675 16.650000000000002 8 17.424999999999997 27.150000000000002 32.2 23.225 9 17.525 24.05 35.025 23.400000000000002 10-14 20.424999999999997 28.95 28.365000000000002 22.259999999999998 15-19 20.915 27.73 27.595 23.76 20-24 20.345 28.585 27.61 23.46 25-29 20.435 28.925 27.125 23.515 30-34 20.01 29.054999999999996 27.339999999999996 23.595 35-39 20.155 28.37 27.73 23.745 40-44 19.895 28.945 28.349999999999998 22.81 45-49 20.685000000000002 27.21 28.555000000000003 23.549999999999997 50-54 20.16 27.48 28.610000000000003 23.75 55-59 19.88 29.375 27.195000000000004 23.549999999999997 60-64 20.495 28.110000000000003 27.944999999999997 23.45 65-69 20.21 28.945 27.529999999999998 23.315 70-74 20.285 28.945 27.21 23.56 75-79 20.665 27.805000000000003 28.205000000000002 23.325000000000003 80-84 20.549999999999997 28.065 27.544999999999998 23.84 85-89 20.29 28.38 27.950000000000003 23.380000000000003 90-94 19.650000000000002 27.994999999999997 28.12 24.235 95-99 20.49 28.945 27.284999999999997 23.28 100-104 20.455000000000002 28.804999999999996 27.544999999999998 23.195 105-109 21.285 29.060000000000002 26.674999999999997 22.98 110-114 20.97 27.62 27.305 24.104999999999997 115-119 20.5 28.79 27.01 23.7 120-124 20.665 28.310000000000002 26.905 24.12 125-129 21.09 28.720000000000002 26.56 23.630000000000003 130-134 20.995 27.845 26.68 24.48 135-139 21.279999999999998 28.105000000000004 26.745 23.87 140-144 21.285 28.525 26.02 24.169999999999998 145-149 21.6 27.725 26.400000000000002 24.275 150-151 22.2125 28.3875 25.95 23.45 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 1.5 22 1.0 23 0.5 24 1.0 25 1.0 26 1.0 27 4.5 28 9.5 29 18.5 30 19.5 31 19.5 32 28.0 33 37.0 34 55.0 35 72.5 36 96.5 37 108.5 38 124.5 39 149.5 40 175.5 41 213.0 42 238.5 43 264.5 44 270.5 45 261.0 46 284.0 47 264.0 48 234.0 49 226.5 50 183.0 51 132.5 52 107.0 53 88.0 54 68.5 55 64.0 56 47.0 57 36.0 58 31.5 59 27.5 60 15.5 61 4.5 62 3.5 63 2.0 64 2.0 65 3.0 66 2.5 67 0.5 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.675 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.675 #Duplication Level Percentage of deduplicated Percentage of total 1 70.367673825992 48.325 2 19.111758281761922 26.25 3 6.771022934109938 13.950000000000001 4 2.6574444848926104 7.3 5 0.6552602839461231 2.25 6 0.2912267928649436 1.2 7 0.10921004732435385 0.525 8 0.03640334910811795 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCCAGCACTGTTTGCCTCGTATGCTGCACAAGCCTCTGGGGTGTCTTCT 8 0.2 No Hit CTCTTTGATCATGAAAAGAACAGAAGCTGCAAACACACTCTGAACTATCT 7 0.17500000000000002 No Hit GTATGAGACTGTAAAGGAATGTTTTTTTATATTATCATCTGTATTCACTG 7 0.17500000000000002 No Hit TTTCGCCAGCCATTGTGAACTTGAAACTGCAGCCTGTAAGGACCATCCCA 7 0.17500000000000002 No Hit CTTGTATTTAACCGAATGGAAGGCCCCATTGATGAGCATATATGTGCGCT 6 0.15 No Hit CAAGGCGTGTTCTTAGGATCACTTCCAGTGATAATCTGCCACTCCTGACC 6 0.15 No Hit CATCGGTGATTGTTACCATACCATAACTTCAAGCCATCGCTGACTTCACT 6 0.15 No Hit GTCGTCATCAGCTGAGGCCTTGGAGGAGCCACCAGTCTTCTTTGGGAGGA 6 0.15 No Hit ATCACACATAGTTTTTTTAATCTATAGCATATCGTCTTGGTTCATTCAGG 6 0.15 No Hit CGGAATCAGAGTCATCATCACTTGAATCAGCTTTCTTCTGGGCAGCTGGT 6 0.15 No Hit GTCAGCTTACTGCCTCTGAAGGGTATGTGACCCTGGTCACTGTCAAGAGC 6 0.15 No Hit CACAATACCAGGAAAGCCCTTTTCTCTGCGAGAAAAGAGTTCACTATCTG 6 0.15 No Hit CCTTGATAGAATTTGCCTGTCTATTGGGGTGCCTCACCTTGAGAGCCAAG 5 0.125 No Hit CACCAAGACGACTGAAGGCATGCTTTAGCTCTTTCTTGCTGAGAAGACCG 5 0.125 No Hit TGCCCACAATGCCCTCAAGAGCATGTAGTACACTGAGGGAAGAAGCAAGA 5 0.125 No Hit GCCTTTTGGCTTGGCGGCAATAAAGCTGATGCACTGCACTTGACGCGTGT 5 0.125 No Hit GCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTA 5 0.125 No Hit TCTACATTAGGGAACTTTTGAGGGAATGGAACTGGTGGTTTCTTCCGCAT 5 0.125 No Hit GCTTGAATATGTTTCCCAGCACCAGGGCCATCCACAAAAACTCCGGTTCC 5 0.125 No Hit CCTCAGCTTCTTTCAATAACTGCATGAACAGCGGGTGATTTATATAAATC 5 0.125 No Hit CCTGGAATGGATGACTTCAAGCTCACGGTTCTTGGCAAAGGTCTCTGGGT 5 0.125 No Hit GCCATGGCTTGCTTTGATTTGACTTGGCTGAAGGGGCTCTAAAATCTGAT 5 0.125 No Hit GCTTTATGTTAAAGTCTGCAGATTATCCCTTCAAGGAGAGCTAGTTTCAC 5 0.125 No Hit TGGCCTTGTTGCAGCTTCTGGGAATCATGGAAAGCAACAGAACTATCTGA 5 0.125 No Hit CTTTTCATTGTTTTCTGAGTATACATCAGCAGTATAGAAGAAAAAAATTT 5 0.125 No Hit GTGCATATATACATCTAATGAAGATACAGATCAAGGTTTGCTGGATTAAC 5 0.125 No Hit CAGCTTTTGGATTTGGCATGAGTCCTCGTGGTCCTAGAATCTTACCTAAG 5 0.125 No Hit GTGCAGCTCTTTGGGGTCACAGAAAAGTTTAGCAGCATCTTGAACATGTA 5 0.125 No Hit GGACCCCTCAAGAAGGAAAATAAAAGAATGTTGCCACACATTACCAGGTC 5 0.125 No Hit GCCAATACATAACAATGAAGAAGACACAAAGATAAAGACACTCACGGACA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0125 0.0 0.0 0.0 60-61 0.05 0.025 0.0 0.0 0.0 62-63 0.125 0.025 0.0 0.0 0.0 64-65 0.175 0.025 0.0 0.0 0.0 66-67 0.25 0.025 0.0 0.0 0.0 68-69 0.2875 0.025 0.0 0.0 0.0 70-71 0.3375 0.025 0.0 0.0 0.0 72-73 0.4625 0.025 0.0 0.0 0.0 74-75 0.6125 0.025 0.0 0.0 0.0 76-77 0.7625 0.025 0.0 0.0 0.0 78-79 0.8125 0.025 0.0 0.0 0.0 80-81 1.0 0.025 0.0 0.0 0.0 82-83 1.1124999999999998 0.025 0.0 0.0 0.0 84-85 1.2375 0.025 0.0 0.0 0.0 86-87 1.4874999999999998 0.025 0.0 0.0 0.0 88-89 1.825 0.025 0.0 0.0 0.0 90-91 2.0250000000000004 0.025 0.0 0.0 0.0 92-93 2.1625 0.025 0.0 0.0 0.0 94-95 2.4749999999999996 0.025 0.0 0.0 0.0 96-97 2.975 0.025 0.0 0.0 0.0 98-99 3.45 0.025 0.0 0.0 0.0 100-101 4.0375 0.025 0.0 0.0 0.0 102-103 4.3625 0.025 0.0 0.0 0.0 104-105 4.8 0.025 0.0 0.0 0.0 106-107 4.987500000000001 0.025 0.0 0.0 0.0 108-109 5.4375 0.025 0.0 0.0 0.0 110-111 5.8625 0.025 0.0 0.0 0.0 112-113 6.4625 0.025 0.0 0.0 0.0 114-115 7.0125 0.025 0.0 0.0 0.0 116-117 7.95 0.025 0.0 0.0 0.0 118-119 8.6125 0.025 0.0 0.0 0.0 120-121 9.162500000000001 0.025 0.0 0.0 0.0 122-123 9.962499999999999 0.025 0.0 0.0 0.0 124-125 10.5875 0.025 0.0 0.0 0.0 126-127 11.375 0.025 0.0 0.0 0.0 128-129 12.4625 0.025 0.0 0.0 0.0 130-131 13.1875 0.025 0.0 0.0 0.0 132-133 13.85 0.025 0.0 0.0 0.0 134-135 14.25 0.025 0.0 0.0 0.0 136-137 15.175 0.025 0.0 0.0 0.0 138-139 16.1375 0.025 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACAAA 10 0.006830828 145.0 1 AGGGGCA 10 0.006830828 145.0 8 GTATTTG 10 0.006830828 145.0 8 GGCTGTA 10 0.006830828 145.0 145 AGTAACG 10 0.006830828 145.0 145 AGCACAA 10 0.006830828 145.0 2 AATATAC 10 0.006830828 145.0 8 AATAAGG 10 0.006830828 145.0 6 GCACAAA 10 0.006830828 145.0 3 >>END_MODULE SRR13695479 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695479_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.26575 37.0 37.0 37.0 37.0 37.0 2 36.2945 37.0 37.0 37.0 37.0 37.0 3 36.3365 37.0 37.0 37.0 37.0 37.0 4 36.3575 37.0 37.0 37.0 37.0 37.0 5 36.4735 37.0 37.0 37.0 37.0 37.0 6 36.3335 37.0 37.0 37.0 37.0 37.0 7 36.3495 37.0 37.0 37.0 37.0 37.0 8 36.416 37.0 37.0 37.0 37.0 37.0 9 36.3835 37.0 37.0 37.0 37.0 37.0 10-14 36.3711 37.0 37.0 37.0 37.0 37.0 15-19 36.386 37.0 37.0 37.0 37.0 37.0 20-24 36.3725 37.0 37.0 37.0 37.0 37.0 25-29 36.280899999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.24850000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.2146 37.0 37.0 37.0 37.0 37.0 40-44 36.26095 37.0 37.0 37.0 37.0 37.0 45-49 36.2303 37.0 37.0 37.0 37.0 37.0 50-54 36.18575 37.0 37.0 37.0 37.0 37.0 55-59 36.166900000000005 37.0 37.0 37.0 37.0 37.0 60-64 36.123900000000006 37.0 37.0 37.0 37.0 37.0 65-69 36.0746 37.0 37.0 37.0 37.0 37.0 70-74 36.0713 37.0 37.0 37.0 37.0 37.0 75-79 36.07255 37.0 37.0 37.0 37.0 37.0 80-84 36.0081 37.0 37.0 37.0 37.0 37.0 85-89 35.942449999999994 37.0 37.0 37.0 37.0 37.0 90-94 35.90875 37.0 37.0 37.0 37.0 37.0 95-99 35.9859 37.0 37.0 37.0 37.0 37.0 100-104 35.9114 37.0 37.0 37.0 37.0 37.0 105-109 35.872749999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.7597 37.0 37.0 37.0 37.0 37.0 115-119 35.718149999999994 37.0 37.0 37.0 37.0 37.0 120-124 35.59 37.0 37.0 37.0 37.0 37.0 125-129 35.611399999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.3707 37.0 37.0 37.0 34.6 37.0 135-139 35.29085 37.0 37.0 37.0 34.6 37.0 140-144 35.1486 37.0 37.0 37.0 29.8 37.0 145-149 34.90275 37.0 37.0 37.0 25.0 37.0 150-151 34.577625 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 2.0 14 5.0 15 2.0 16 0.0 17 1.0 18 1.0 19 1.0 20 1.0 21 0.0 22 1.0 23 3.0 24 3.0 25 2.0 26 4.0 27 10.0 28 16.0 29 13.0 30 20.0 31 43.0 32 75.0 33 107.0 34 219.0 35 525.0 36 2706.0 37 239.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.04092392668843 21.441124780316343 13.256339442631182 26.261611850364048 2 27.125 26.700000000000003 31.1 15.075 3 21.275 28.625 31.175000000000004 18.925 4 24.275 34.725 23.200000000000003 17.8 5 25.575 35.199999999999996 22.675 16.55 6 19.125 38.324999999999996 23.225 19.325 7 19.650000000000002 22.0 39.625 18.725 8 22.025 25.775 26.375 25.825 9 23.474999999999998 24.275 30.099999999999998 22.15 10-14 22.48 29.110000000000003 26.845000000000002 21.565 15-19 22.919999999999998 27.950000000000003 28.03 21.099999999999998 20-24 22.177217721772177 28.722872287228725 27.677767776777678 21.422142214221424 25-29 23.13656828414207 28.854427213606805 28.194097048524263 19.814907453726864 30-34 22.58177453235971 28.39851955586676 28.018405521656497 21.001300390117038 35-39 22.927292729272928 28.17781778177818 28.347834783478348 20.547054705470547 40-44 22.735683920980247 27.33183295823956 28.977244311077772 20.955238809702426 45-49 22.309461892378476 28.085617123424683 28.530706141228247 21.074214842968594 50-54 23.071153557677885 27.731386569328464 28.346417320866042 20.85104255212761 55-59 22.696348174087046 28.274137068534266 27.54377188594297 21.48574287143572 60-64 22.892289228922895 28.36283628362836 27.33273327332733 21.412141214121412 65-69 22.38 27.305 28.63 21.685 70-74 23.196598299149578 28.784392196098047 27.12856428214107 20.890445222611305 75-79 23.41319461811634 28.22487870754764 27.99479817936278 20.36712849497324 80-84 23.125 28.875 27.065 20.935000000000002 85-89 23.575609024060828 28.797959081586715 27.89255164824171 19.73388024611075 90-94 23.643546531979798 27.71915787368105 27.71915787368105 20.9181377206581 95-99 24.127412741274128 28.642864286428644 27.04270427042704 20.187018701870187 100-104 24.41720860430215 28.394197098549274 26.793396698349177 20.3951975987994 105-109 23.94577559901956 28.627882547146218 27.032164474013303 20.394177379820917 110-114 24.59245924592459 28.937893789378936 26.782678267826782 19.686968696869688 115-119 24.61361476516781 27.974791176911918 27.364577602160757 20.04701645575952 120-124 25.002501250625315 28.264132066033014 27.008504252126066 19.72486243121561 125-129 25.21756526958087 27.97339201760528 26.44293287986396 20.366109832949885 130-134 25.925555333199917 27.281368821292773 26.746047628577145 20.04702821693016 135-139 25.749162039121515 27.38506178398119 27.27500125068788 19.590774926209413 140-144 26.732673267326735 27.667766776677666 26.172617261726174 19.426942694269428 145-149 27.154935214367903 27.08989944469458 26.839761869027967 18.91540347190955 150-151 27.836857250093832 26.585762542224444 25.88514950581759 19.69223070186413 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.5 20 1.0 21 1.0 22 0.5 23 3.0 24 4.0 25 2.5 26 6.0 27 7.5 28 6.0 29 12.0 30 23.5 31 26.5 32 28.0 33 38.5 34 50.5 35 64.5 36 76.5 37 117.5 38 158.0 39 173.5 40 218.0 41 244.0 42 256.0 43 263.5 44 267.0 45 275.5 46 262.0 47 232.5 48 195.0 49 182.5 50 176.5 51 135.5 52 96.0 53 84.5 54 79.0 55 58.5 56 40.5 57 34.0 58 26.0 59 19.5 60 19.5 61 15.5 62 4.5 63 2.5 64 1.0 65 0.0 66 0.5 67 0.5 68 0.0 69 0.5 70 1.5 71 1.0 72 0.0 73 0.0 74 0.0 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42500000000000004 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.01 25-29 0.05 30-34 0.03 35-39 0.01 40-44 0.025 45-49 0.02 50-54 0.005 55-59 0.05 60-64 0.01 65-69 0.0 70-74 0.05 75-79 0.034999999999999996 80-84 0.0 85-89 0.045 90-94 0.015 95-99 0.01 100-104 0.05 105-109 0.045 110-114 0.01 115-119 0.034999999999999996 120-124 0.05 125-129 0.03 130-134 0.06 135-139 0.055 140-144 0.01 145-149 0.055 150-151 0.08750000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.525 #Duplication Level Percentage of deduplicated Percentage of total 1 70.37577526450201 48.225 2 18.97117840204305 26.0 3 6.8588106530463335 14.099999999999998 4 2.553812477198103 7.000000000000001 5 0.7661437431594309 2.625 6 0.36483035388544327 1.5 7 0.07296607077708865 0.35000000000000003 8 0.036483035388544326 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTAAATAAAGAGATAGTGAAGGTCCAAAGAGTAACTGCATCTGTTGGCCA 8 0.2 No Hit GGAAAACCATGAACGGCCTTCCTCCATCTTCTGGGGAAAACCTAAAGCTT 7 0.17500000000000002 No Hit GGAGATTGGTGGAAATAACCTCTTAAGATACTCAGGAACTCTTGATGCGA 7 0.17500000000000002 No Hit GGCGAAGGATGCTGGAGAGGGAAAAGGAAACAATAGAGAAGAAGCCATTC 6 0.15 No Hit AACAGACAAGAATAGAAGGTGCTGAAATTAATAAGAGCTTACTCGCCCTG 6 0.15 No Hit ATAGAACAGAACTTGACCACTGAGTTCATTCTTGGTATCCGTAATAAGGA 6 0.15 No Hit GACAGCTCCATGCCAGAAGTCCCCGAGATACAGGTTCAATCTGCACCAGA 6 0.15 No Hit GGGAGCCCATGGTACGGACCAGACCGTGTTAAATACTTGGGCCCGTTCTC 6 0.15 No Hit AAGAGATCAGTCAAATGCGATATTGGATTTCATTGAAGCCAAGTGGTCTG 6 0.15 No Hit AAGCTGATTTCTCAGAGGAAGACCTCCTTGTAAACTTGCTAGCTGCGGTA 6 0.15 No Hit GAGAAAGGTTGGGTCTACCGTGAGCACCACAGCTCACCAGGGTACTATGA 6 0.15 No Hit CATGTCTTGTCTGGACACTTGAGCAGGTTGGTTGCTCTTTTCAGCTGTTG 6 0.15 No Hit GAAAGCTACTCCTGCCAAGAAGGAGCCAATTGCTGTAAAAAATGGTTCCC 6 0.15 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit TCCCGTTGAGGCATACAAAACACTAATTTCTTTGAAGTGTGTTACTGGGT 5 0.125 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 5 0.125 No Hit GTTGGCTGGATACTGGTTTGCCATTGTCCATTTTTTTGTGCGGTAACATG 5 0.125 No Hit GGATGTCAAGATCCAGGGGATCTGGTATGCTCAGCTTGATCAATAGAGCT 5 0.125 No Hit GTCCATTTCTCCATGCATGCCAGTTGAAGGAGTGGGAGAGGCCGAAGACC 5 0.125 No Hit AGTTTCTTGCACACCAATAACCACAAAGAGACCAAGCAGTGTTAAGCCAT 5 0.125 No Hit ATTAAAATCCCAACTATACCAGAGAATATCCCAATTATCCATAAAACTGT 5 0.125 No Hit CATAACACGAATGGGTGGATTTCGAAGGATTGCGACATTAATGAATCTCT 5 0.125 No Hit TATTAATCATAGATTACCTGAGCAGCATTTTCTATGAAATGTTGTAGCAT 5 0.125 No Hit AACAGGACAAACCGTTAAAGTTGCAGTGCTCACCCAAGGTGAAAAGTTCG 5 0.125 No Hit CCCAAACCACTTGTTTCTCAGCTAACTGAAGAGCAGTTGAGGGCAATTTT 5 0.125 No Hit CTAGAGGTTCTCCTTTTGGCACGCGGAGGTGTTTTTCAATGTGAAATTTG 5 0.125 No Hit CAGCATTTATCCTCTCCCAAAAGAAATACTCTCTCCACATTTCGGTTCGA 5 0.125 No Hit TATGGTATTACTCTATTCTCAATCATGTGTTCATTGCACTGCTACTATGT 5 0.125 No Hit GTTAAAGAGTTGCTTCAATGGAATGGTAAGTGGCATTTTATTCACTACTA 5 0.125 No Hit CATGTATCAAGGATGCAGATGCGAATTTTAGTGCTTTTGTTACCTGTTAA 5 0.125 No Hit GTTTGTAGTGAATGTTCTGCTTCTTTAAATTCCATCTCGACTCCAGCACC 5 0.125 No Hit TTGTTGTCAATGACAGCGGTGGTGTCAAGAACGCTTCCCACTTGTTGAAA 5 0.125 No Hit CTCCTGCAATTGATATTTGGAGCATTGGATGTATATTTGCTGAAATGCTC 5 0.125 No Hit ATCACCACCACCACCACCACCATGATCATCATGGCAAGAAGCAGTTGAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.175 0.0 0.0 0.0 0.0 66-67 0.25 0.0 0.0 0.0 0.0 68-69 0.2875 0.0 0.0 0.0 0.0 70-71 0.3375 0.0 0.0 0.0 0.0 72-73 0.4625 0.0 0.0 0.0 0.0 74-75 0.6125 0.0 0.0 0.0 0.0 76-77 0.7375 0.0 0.0 0.0 0.0 78-79 0.7875 0.0 0.0 0.0 0.0 80-81 0.975 0.0 0.0 0.0 0.0 82-83 1.0875 0.0 0.0 0.0 0.0 84-85 1.2125 0.0 0.0 0.0 0.0 86-87 1.4625 0.0 0.0 0.0 0.0 88-89 1.775 0.0 0.0 0.0 0.0 90-91 1.975 0.0 0.0 0.0 0.0 92-93 2.1125 0.0 0.0 0.0 0.0 94-95 2.4125 0.0 0.0 0.0 0.0 96-97 2.9 0.0 0.0 0.0 0.0 98-99 3.375 0.0 0.0 0.0 0.0 100-101 3.9625000000000004 0.0 0.0 0.0 0.0 102-103 4.2875 0.0 0.0 0.0 0.0 104-105 4.7125 0.0 0.0 0.0 0.0 106-107 4.9125 0.0 0.0 0.0 0.0 108-109 5.35 0.0 0.0 0.0 0.0 110-111 5.7625 0.0 0.0 0.0 0.0 112-113 6.3625 0.0 0.0 0.0 0.0 114-115 6.9125 0.0 0.0 0.0 0.0 116-117 7.8500000000000005 0.0 0.0 0.0 0.0 118-119 8.5375 0.0 0.0 0.0 0.0 120-121 9.087499999999999 0.0 0.0 0.0 0.0 122-123 9.912500000000001 0.0 0.0 0.0 0.0 124-125 10.5375 0.0 0.0 0.0 0.0 126-127 11.35 0.0 0.0 0.0 0.0 128-129 12.425 0.0 0.0 0.0 0.0 130-131 13.1125 0.0 0.0 0.0 0.0 132-133 13.775 0.0 0.0 0.0 0.0 134-135 14.175 0.0 0.0 0.0 0.0 136-137 15.1 0.0 0.0 0.0 0.0 138-139 16.0625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAAGG 10 0.006830828 145.0 6 GTTTGTA 10 0.006830828 145.0 1 GGTTGTG 10 0.006830828 145.0 145 GTAGAAG 10 0.006830828 145.0 5 GAGCTCA 10 0.006830828 145.0 9 AAAATTG 10 0.006830828 145.0 2 TTTGTAG 10 0.006830828 145.0 2 ATTGGAG 25 8.7132835E-4 87.0 5 >>END_MODULE Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850138 spots for SRR13695479.sra Written 850138 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra Read 850130 spots for SRR13695479.sra Written 850130 spots for SRR13695479.sra SRR ids: ['SRR13695479.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_z_4obf63 SRR13695479.sra spots: 17002608 blocks: [[1, 850130], [850131, 1700260], [1700261, 2550390], [2550391, 3400520], [3400521, 4250650], [4250651, 5100780], [5100781, 5950910], [5950911, 6801040], [6801041, 7651170], [7651171, 8501300], [8501301, 9351430], [9351431, 10201560], [10201561, 11051690], [11051691, 11901820], [11901821, 12751950], [12751951, 13602080], [13602081, 14452210], [14452211, 15302340], [15302341, 16152470], [16152471, 17002608]] SRR13695479 file size 5756529 SRR13695479 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695479 SRR13695479_1.fastq SRR13695479_2.fastq Input file: SRR13695479_1.fastq Paired file: SRR13695479_2.fastq trimmed: SRR13695479-trimmed-pair1.fastq, SRR13695479-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 06:22:55 2025 >> started Wed Feb 12 06:23:16 2025 >> done (20.552s) 17002608 read pairs processed; of these: 125 ( 0.00%) short read pairs filtered out after trimming by size control 2692 ( 0.02%) empty read pairs filtered out after trimming by size control 16999791 (99.98%) read pairs available; of these: 3388924 (19.94%) trimmed read pairs available after processing 13610867 (80.06%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 0 0.00% 20 2 0.00% 21 3 0.00% 22 3 0.00% 23 2 0.00% 24 2 0.00% 25 1 0.00% 26 3 0.00% 27 3 0.00% 28 1 0.00% 29 2 0.00% 30 5 0.00% 31 2 0.00% 32 8 0.00% 33 4 0.00% 34 4 0.00% 35 6 0.00% 36 20 0.00% 37 9 0.00% 38 11 0.00% 39 25 0.00% 40 28 0.00% 41 33 0.00% 42 34 0.00% 43 30 0.00% 44 49 0.00% 45 46 0.00% 46 65 0.00% 47 78 0.00% 48 103 0.00% 49 167 0.00% 50 175 0.00% 51 166 0.00% 52 253 0.00% 53 272 0.00% 54 260 0.00% 55 336 0.00% 56 380 0.00% 57 468 0.00% 58 583 0.00% 59 681 0.00% 60 902 0.01% 61 1068 0.01% 62 1201 0.01% 63 1319 0.01% 64 1571 0.01% 65 1789 0.01% 66 1981 0.01% 67 2304 0.01% 68 2705 0.02% 69 3082 0.02% 70 3363 0.02% 71 4249 0.02% 72 4814 0.03% 73 5525 0.03% 74 6027 0.04% 75 6613 0.04% 76 7146 0.04% 77 7682 0.05% 78 8819 0.05% 79 9528 0.06% 80 10283 0.06% 81 11748 0.07% 82 12473 0.07% 83 13995 0.08% 84 15546 0.09% 85 16572 0.10% 86 17397 0.10% 87 18194 0.11% 88 19333 0.11% 89 19958 0.12% 90 21670 0.13% 91 22524 0.13% 92 23793 0.14% 93 25701 0.15% 94 26566 0.16% 95 28647 0.17% 96 29734 0.17% 97 30542 0.18% 98 30923 0.18% 99 32176 0.19% 100 33238 0.20% 101 33746 0.20% 102 35615 0.21% 103 37082 0.22% 104 38154 0.22% 105 39759 0.23% 106 40888 0.24% 107 41492 0.24% 108 42937 0.25% 109 43691 0.26% 110 43673 0.26% 111 45395 0.27% 112 46500 0.27% 113 47352 0.28% 114 48827 0.29% 115 50187 0.30% 116 51446 0.30% 117 52053 0.31% 118 53084 0.31% 119 53492 0.31% 120 54759 0.32% 121 55455 0.33% 122 55641 0.33% 123 56741 0.33% 124 57517 0.34% 125 58134 0.34% 126 59660 0.35% 127 60430 0.36% 128 60686 0.36% 129 62095 0.37% 130 61969 0.36% 131 61954 0.36% 132 62945 0.37% 133 63932 0.38% 134 63882 0.38% 135 64812 0.38% 136 65636 0.39% 137 65846 0.39% 138 66849 0.39% 139 68293 0.40% 140 67860 0.40% 141 68700 0.40% 142 68770 0.40% 143 68719 0.40% 144 69084 0.41% 145 70436 0.41% 146 69902 0.41% 147 70808 0.42% 148 72248 0.42% 149 70988 0.42% 150 71774 0.42% 151 13610867 80.06% 16999791 reads passed initial QC criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=2.48 fanout-score-rank=28 prefix-density=0.38 prefix-fanout=2.3 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAGCTTCCACATAGTCCAGTAGCGTCCATCATAGTACCCTGGTGAGCTGTGGTGCTCACGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGTAATCAATTTCCTTGGCCAATTCCTCCTCTGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTTGGTGGCCACACCTGCATGCATTGAACTCGTCCACCATTGCTTGCAATGGAAGTAATGTCATT criterion=fanout-score sequence-density=0.02 sequence-density-rank=35 fanout-score=197.04 fanout-score-rank=1 prefix-density=0.24 prefix-fanout=15.3 sequence=CTGCTGCTGCATTTATTAGAGAAGGAGATGCTGGTAATCTCCATTTCACAAGTTCATTAATCTCGATCGAAAATATGGCTCATTTAAGCATATACAAAGTACATCTGGGAAAGAAAGTTAAGAACCAAGATAGGGTCACTGATATTTGGATGATGTTCATACGGAGAGGGAGGGAGAAAGGCTGTACTCAAGCCCCCAGAGCTCAAAACTGCCTTTGACAAAATCATAATAACCACCCTTCAGTCCTAGAGTTTTGTTCACCAAGCCATCTCTCACAAACGGGTAGGTTAGCAAGTGTCCAAGGGACACGTTCACTGCCTCCTTTTCACATTGTGTACAGAGGTCTGGGAAAGGTGCATTGGCATGTTCTGCTAAAACCTTAGTCTTGGCAGGGTAACAAACTTTGACCCAGTCTTCTATGAAATCAGTTGATGTTGTTCCATCATAAGGGAAGGACATGAGGCCCTTAATTCCACCACAGGCGCTGTGTCCAATGACCACAATGTATTCC criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=2.19 fanout-score-rank=36 prefix-density=0.43 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=65.61 fanout-score-rank=1 prefix-density=0.32 prefix-fanout=2.5 sequence=TTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGG SRR13695479 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 06:23:57 Started mapping on | Feb 12 06:23:57 Finished on | Feb 12 06:26:09 Mapping speed, Million of reads per hour | 463.63 Number of input reads | 16999791 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 15784707 Uniquely mapped reads % | 92.85% Average mapped length | 289.06 Number of splices: Total | 15372394 Number of splices: Annotated (sjdb) | 15040246 Number of splices: GT/AG | 15060181 Number of splices: GC/AG | 253837 Number of splices: AT/AC | 9003 Number of splices: Non-canonical | 49373 Mismatch rate per base, % | 0.36% Deletion rate per base | 0.03% Deletion average length | 3.03 Insertion rate per base | 0.02% Insertion average length | 2.30 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 387996 % of reads mapped to multiple loci | 2.28% Number of reads mapped to too many loci | 58243 % of reads mapped to too many loci | 0.34% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.39% % of reads unmapped: other | 0.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 827285 827285 827285 N_multimapping 387996 387996 387996 N_noFeature 608706 15377204 874372 N_ambiguous 232932 1863 89754 UnstrandedReadsAssigned:14943069 PositiveStrandReadsAssigned:405640 NegativeStrandReadsAssigned:14820581 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695479 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695479-trimmed-pair1.fastq SRR13695479-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,999,791 reads, 14,869,330 reads pseudoaligned [quant] estimated average fragment length: 224.851 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,127 rounds 52401 SRR13695479.ke.tsv 34699 SRR13695479.se.tsv 87100 total ==> SRR13695479.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1794.15 667 23.9994 Potri.005G024800.1.v4.1 1035 811.149 336 26.7407 Potri.004G059700.1.v4.1 961 737.205 4 0.350272 Potri.007G009000.2.v4.1 1416 1192.15 0 0 Potri.003G141000.2.v4.1 2943 2719.15 996.128 23.6492 Potri.016G087400.1.v4.1 270 98.2744 704.413 462.722 Potri.015G069301.1.v4.1 564 347.157 0 0 Potri.010G195200.1.v4.1 1773 1549.15 92 3.83379 Potri.012G127500.1.v4.1 977 753.174 66 5.65695 ==> SRR13695479.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 123 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 264 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 2 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 8 Potri.001G416900.v4.1 1 Potri.001G452600.v4.1 7 SRR13695479 completed mapping pipeline successfully