Starting /dee2/code/volunteer_pipeline.sh SRR13695480 current disk space = 3050289741824 free memory = 1311969508 SRR13695480 SRAfilesize 2212d42126a2daa18b7e41fa8fe0ac5e SRR13695480.sra SRR13695480.sra file validated SRR13695480 is paired end SRR13695480 is conventional basespace SRR13695480 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695480_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5775 37.0 37.0 37.0 37.0 37.0 2 36.321 37.0 37.0 37.0 37.0 37.0 3 36.66 37.0 37.0 37.0 37.0 37.0 4 36.611 37.0 37.0 37.0 37.0 37.0 5 36.631 37.0 37.0 37.0 37.0 37.0 6 36.5855 37.0 37.0 37.0 37.0 37.0 7 36.486 37.0 37.0 37.0 37.0 37.0 8 36.631 37.0 37.0 37.0 37.0 37.0 9 36.586 37.0 37.0 37.0 37.0 37.0 10-14 36.6063 37.0 37.0 37.0 37.0 37.0 15-19 36.576 37.0 37.0 37.0 37.0 37.0 20-24 36.5689 37.0 37.0 37.0 37.0 37.0 25-29 36.5001 37.0 37.0 37.0 37.0 37.0 30-34 36.4525 37.0 37.0 37.0 37.0 37.0 35-39 36.4227 37.0 37.0 37.0 37.0 37.0 40-44 36.4131 37.0 37.0 37.0 37.0 37.0 45-49 36.3935 37.0 37.0 37.0 37.0 37.0 50-54 36.361 37.0 37.0 37.0 37.0 37.0 55-59 36.3695 37.0 37.0 37.0 37.0 37.0 60-64 36.33240000000001 37.0 37.0 37.0 37.0 37.0 65-69 36.270599999999995 37.0 37.0 37.0 37.0 37.0 70-74 36.2699 37.0 37.0 37.0 37.0 37.0 75-79 36.2707 37.0 37.0 37.0 37.0 37.0 80-84 36.228100000000005 37.0 37.0 37.0 37.0 37.0 85-89 36.185700000000004 37.0 37.0 37.0 37.0 37.0 90-94 36.1415 37.0 37.0 37.0 37.0 37.0 95-99 36.0982 37.0 37.0 37.0 37.0 37.0 100-104 36.1104 37.0 37.0 37.0 37.0 37.0 105-109 36.0957 37.0 37.0 37.0 37.0 37.0 110-114 36.0575 37.0 37.0 37.0 37.0 37.0 115-119 36.0601 37.0 37.0 37.0 37.0 37.0 120-124 35.979200000000006 37.0 37.0 37.0 37.0 37.0 125-129 35.9381 37.0 37.0 37.0 37.0 37.0 130-134 35.907799999999995 37.0 37.0 37.0 37.0 37.0 135-139 35.9136 37.0 37.0 37.0 37.0 37.0 140-144 35.8718 37.0 37.0 37.0 37.0 37.0 145-149 35.67979999999999 37.0 37.0 37.0 37.0 37.0 150-151 35.486000000000004 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 4.0 20 0.0 21 1.0 22 1.0 23 1.0 24 1.0 25 1.0 26 4.0 27 9.0 28 15.0 29 21.0 30 28.0 31 28.0 32 48.0 33 68.0 34 127.0 35 336.0 36 2955.0 37 352.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.300000000000004 12.75 9.625 37.325 2 20.962888665997994 13.440320962888666 35.05516549648947 30.541624874623874 3 19.425 17.65 26.650000000000002 36.275 4 22.925 23.724999999999998 23.125 30.225 5 24.75 29.95 23.599999999999998 21.7 6 21.525 35.675000000000004 22.05 20.75 7 15.049999999999999 29.025000000000002 40.225 15.7 8 18.525 26.400000000000002 31.874999999999996 23.200000000000003 9 17.375 26.700000000000003 33.7 22.225 10-14 19.655 29.28 28.1 22.965 15-19 19.965 28.23 28.165000000000003 23.64 20-24 19.86 28.249999999999996 27.99 23.9 25-29 20.474999999999998 28.599999999999998 27.985 22.939999999999998 30-34 19.845 29.225 27.800000000000004 23.13 35-39 19.8 29.115000000000002 27.855 23.23 40-44 19.52 28.73 28.689999999999998 23.06 45-49 19.919999999999998 28.395 27.96 23.724999999999998 50-54 20.115 28.93 27.48 23.474999999999998 55-59 20.165 27.894999999999996 27.98 23.96 60-64 20.96 28.505000000000003 27.155 23.380000000000003 65-69 20.48 27.91 27.884999999999998 23.724999999999998 70-74 19.77 28.044999999999998 27.855 24.33 75-79 19.85 27.865000000000002 28.299999999999997 23.985 80-84 20.4 27.82 27.77 24.01 85-89 20.979999999999997 27.839999999999996 28.09 23.09 90-94 21.025 28.27 27.825 22.88 95-99 20.955 28.860000000000003 27.07 23.115 100-104 20.395 29.095 27.02 23.49 105-109 20.59 28.134999999999998 28.07 23.205000000000002 110-114 19.73 28.939999999999998 27.455000000000002 23.875 115-119 21.060000000000002 28.555000000000003 27.16 23.225 120-124 20.945 27.37 27.58 24.104999999999997 125-129 20.69 28.71 27.255000000000003 23.345 130-134 20.9 28.549999999999997 26.779999999999998 23.77 135-139 21.275 28.799999999999997 27.02 22.905 140-144 21.445 27.73 26.88 23.945 145-149 21.884999999999998 27.500000000000004 26.840000000000003 23.775 150-151 21.825 26.9625 26.337500000000002 24.875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 1.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 0.5 20 0.5 21 1.0 22 1.5 23 2.5 24 2.5 25 4.5 26 7.0 27 4.5 28 10.0 29 15.0 30 13.5 31 28.5 32 40.5 33 35.0 34 44.0 35 71.5 36 98.5 37 127.5 38 149.0 39 156.5 40 176.5 41 211.0 42 226.5 43 245.0 44 258.0 45 227.0 46 241.5 47 273.0 48 236.0 49 208.0 50 181.5 51 148.0 52 135.0 53 102.5 54 75.0 55 67.0 56 55.5 57 41.0 58 24.5 59 17.0 60 12.5 61 5.5 62 4.0 63 3.0 64 1.0 65 0.5 66 1.5 67 1.5 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.3 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.14999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 68.38066001534919 44.55 2 19.148119723714505 24.95 3 7.329240214888719 14.325 4 2.9930928626247124 7.8 5 1.2279355333844972 4.0 6 0.38372985418265537 1.5 7 0.2686108979278588 1.225 8 0.11511895625479662 0.6 9 0.03837298541826554 0.22499999999999998 >10 0.11511895625479662 0.8250000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TAATGCGTGTGCTTATTTTCGGAACAAAGGCACCAAAGGGTTGTCTCCAT 12 0.3 No Hit CAGGCAAATAAGAAATAACATGTAAATTAGAATAACTTTTGCAAGTGATA 11 0.27499999999999997 No Hit CTGCAAGTTCACTAGGTGTAATGATTGGTACATCACTCTTTGGTGGTGCA 10 0.25 No Hit ATCCGGCACACTCTTAAATACACTGCCAACGGAAAGAATACCAAATCTGA 9 0.22499999999999998 No Hit CCGTAACTGATCGAGCTGCAGTTGGTGCTGTGTACTTTTTCAGTCTCAAT 8 0.2 No Hit CCCTCATTAGACATTATAGCTTTTCCCTCATAATAGAATGCTTGTAGTGC 8 0.2 No Hit GCCACACGAGTGGTGTTGCTAGGCACATTCAACAACCTGTTTGAACCACT 8 0.2 No Hit GCGCATAAGTTTCAAGCTATCATGGTGTCTTTATTTACACATGACAGAAG 7 0.17500000000000002 No Hit GCTTGATCAGGATCCTTTGCCAAGCCCAGTGGGTCAAAAGGACCACCCGG 7 0.17500000000000002 No Hit GCTGGTTTAGGATTTGTTCCCGAATCCTTATTTGAAGTCATCTTGAACCA 7 0.17500000000000002 No Hit TAGAGGAGGATATATAGCAGAAGACTAGCCTTTAGAAATCTTACTGTTTA 7 0.17500000000000002 No Hit AGAGAGAAGATTCATGAAGATCTAGGTAATTGAATTTTATTTTATTTTTG 7 0.17500000000000002 No Hit GGGGAAAGAGATGAGGAAGAAGAAGGAGTGAAGGCTGTGCTCTGCATTTT 7 0.17500000000000002 No Hit GTCTCAGCACAGAAAAAACTCTTTTGTTGCTCACTTTCCGGGAACGAAGT 7 0.17500000000000002 No Hit GTCGGATTAGCTCTTCTTTGATTAGCATGAAACTCCAAGGTCCGGGGGGG 6 0.15 No Hit CGTATGGCGGGACCATATTCGCAACATTGCGGAGCACAAAAGCTTCCCCT 6 0.15 No Hit CCCATATGCTCATGGTATTATAGTCATCTATTAACTTGTCACGAGCTCCT 6 0.15 No Hit GTCTGGCACGCTCTTTTTCAATAGCTGCTTTGTTTCCAATCTTGTAAATG 6 0.15 No Hit AGCTGGACTTGGATGAGTAGAATGATTTTTCTGCCCTTCCATGGCTTCAC 6 0.15 No Hit GTGGGGTTCTTGCATAGGAGAATCCAGTGCTAACAGGCAAATCTAAGAAT 6 0.15 No Hit TGGGTCGATACATCATATGCATCCGAAGAACTGTTCCAAGGATGGAGAAC 6 0.15 No Hit AATGCTTTGACAGTTTGGAACAACGTTTTTTTACACAAGTGACCAAGATA 6 0.15 No Hit GTGGGAACCCGGCATTGTTCTTGAAGACAATCTTTTCACCAGCGGGTACA 6 0.15 No Hit GTCTGCCCAACCCAAATGGGTCAAACCCGTAATCACCAACCAAACTGCCA 6 0.15 No Hit CCTATCTACATGTTACCAAGAAAAAAGCAGACTAAGTTGACACCTCTTGA 5 0.125 No Hit ACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGC 5 0.125 No Hit CCAAGTCTATTGCTCGAACTGAGGTTGCAGAAGCAAATCTATCGAAGTTG 5 0.125 No Hit AGATATTTGGGAGTGGAGAGGTTTGGTTCAGAGATGGGAATTTCATGACT 5 0.125 No Hit GCTGCTCTAGAAGAGCAAGCTCTCCGAACCTCTTTGAGATCCTGCCCTCA 5 0.125 No Hit CCACATTAATATGTCCTTTCATGGAGTAACCAGTGCCTTTTCCTCTAATC 5 0.125 No Hit CCACAAACTCCTTCTGACCACGCCATTCCATGGCATGAACCCATCTTGAA 5 0.125 No Hit ATCTGGGTCAGCAGCTAAGCCAAGAGGATCAAAGAAATTGCCTCCTGGGT 5 0.125 No Hit GTGCAGCAAAGTCAATGTTCTTCTCTCTAAACTTCTGAAGAAGCTCTTGG 5 0.125 No Hit GGGGAGAATTTTTTGTTTCTGGACAGTGAATGGGACAGGCTATATGATAC 5 0.125 No Hit GTCTGGTAGACAACAGAATCTGCCTGGTTCTTTGTGTCAATGGCATCTCT 5 0.125 No Hit GCTTTATCTTTAGGATTGCTATCACTTGTTTCCGTGGTATAAAGCAAAAT 5 0.125 No Hit GGCGGGAGTTTAATTGCACAAAGCTGAGATATGAAGTTTGACAATGCCAT 5 0.125 No Hit ATATAACTCCGCAGCTCTGGATGTATACTTGGCCTCAGTTTTCCCCCCAT 5 0.125 No Hit GTCCGGATTCGCTTAAAGTCTTCAAATCCTGCTTTCATCGCATCTTCAAG 5 0.125 No Hit GCCTCATGTTTTGATGTGTTTCCTTTCTGAGAAGATGGTTCTTTCTTGCT 5 0.125 No Hit CCTGCAGCTAGTAGAAGACAAGCCTGGCCAATAGCAGCTCCTACAGCAAC 5 0.125 No Hit TCTGCAACTAGCCATAGATTCACATAGTCTTTGAGGTATTTCTCGAATTA 5 0.125 No Hit GTGGTGATAGGGTGAGTACACCCAGAGGAGTTTTCGTCATCATCTCTACT 5 0.125 No Hit GTCCGCCCTGTTAGTTGCCGCAATAACAATGACGCCAGTATTACCCTCAA 5 0.125 No Hit GACCGTAGTGCTTCTCCACCGTGAGCCAGGAGTGTACTGGTGGAACTCCA 5 0.125 No Hit GTCATGATGAATTCTGAGTTGAGACAACCTGCGTTGTGGATGCATGAGAA 5 0.125 No Hit CCTCATCACCTCCTTCAGCTGAAGGATTTGCACCAATGTCTACATCAACG 5 0.125 No Hit GGCTCTTCTTCTTTCTTTTCTTCCTTCTTCTCTTCTTCCTTAGCCTCTTC 5 0.125 No Hit GCTGGATGGAGCCATCAGCTTCACCGTTAACCTCTTCTTCTTGAGATACC 5 0.125 No Hit GTAGAATGAGCATATGGCGAAGTCCCACCTGCCTGAAGAGCACCATGGCT 5 0.125 No Hit GGGCAGCCTCTATTGCTTTAGGTGGCAATCGTATTTGAGGAAATGAATAC 5 0.125 No Hit CCTAGCCTATGGAAATTTGAAGGTCTTCCATTTGCACAGTTCAAAGCCCT 5 0.125 No Hit CCTCCGGCTCCATATCCTACTCGTCGTCGTCATCTTCCTCCCCACTTCCC 5 0.125 No Hit TGTGAACTTGACCTCAATTGGGCTAACGTTCTTTCCGATTGACCTGAAAT 5 0.125 No Hit GTTCAGACGAGGCCCAATTGAGCACCATCCCCCTCGGGTAGTTTCCCGAT 5 0.125 No Hit CCACGAAATACAACATTAAAGAAACCATTTTGAAACCAGAGTTGAATACC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.1875 0.0 0.0 0.0 0.0 86-87 0.3125 0.0 0.0 0.0 0.0 88-89 0.35 0.0 0.0 0.0 0.0 90-91 0.4 0.0 0.0 0.0 0.0 92-93 0.45 0.0 0.0 0.0 0.0 94-95 0.5625 0.0 0.0 0.0 0.0 96-97 0.7 0.0 0.0 0.0 0.0 98-99 0.8875 0.0 0.0 0.0 0.0 100-101 1.15 0.0 0.0 0.0 0.0 102-103 1.3250000000000002 0.0 0.0 0.0 0.0 104-105 1.4875 0.0 0.0 0.0 0.0 106-107 1.625 0.0 0.0 0.0 0.0 108-109 1.8624999999999998 0.0 0.0 0.0 0.0 110-111 2.2375 0.0 0.0 0.0 0.0 112-113 2.6625 0.0 0.0 0.0 0.0 114-115 3.1500000000000004 0.0 0.0 0.0 0.0 116-117 3.5625 0.0 0.0 0.0 0.0 118-119 4.15 0.0 0.0 0.0 0.0 120-121 4.75 0.0 0.0 0.0 0.0 122-123 5.7 0.0 0.0 0.0 0.0 124-125 6.2125 0.0 0.0 0.0 0.0 126-127 6.8875 0.0 0.0 0.0 0.0 128-129 7.625 0.0 0.0 0.0 0.0 130-131 8.1625 0.0 0.0 0.0 0.0 132-133 8.9125 0.0 0.0 0.0 0.0 134-135 9.5875 0.0 0.0 0.0 0.0 136-137 10.3 0.0 0.0 0.0 0.0 138-139 11.0125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCTTTT 10 0.006830828 145.0 3 GGGAAAG 10 0.006830828 145.0 2 GCTTTTC 10 0.006830828 145.0 4 GGGGAAA 10 0.006830828 145.0 1 AAAGATG 10 0.006830828 145.0 145 CCGAATA 10 0.006830828 145.0 3 >>END_MODULE SRR13695480 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695480_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.19325 37.0 37.0 37.0 37.0 37.0 2 36.1295 37.0 37.0 37.0 37.0 37.0 3 36.016 37.0 37.0 37.0 37.0 37.0 4 36.2265 37.0 37.0 37.0 37.0 37.0 5 36.2735 37.0 37.0 37.0 37.0 37.0 6 36.3045 37.0 37.0 37.0 37.0 37.0 7 36.295 37.0 37.0 37.0 37.0 37.0 8 36.215 37.0 37.0 37.0 37.0 37.0 9 36.3495 37.0 37.0 37.0 37.0 37.0 10-14 36.261199999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.190999999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.16065 37.0 37.0 37.0 37.0 37.0 25-29 36.15055 37.0 37.0 37.0 37.0 37.0 30-34 36.11115000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.13695 37.0 37.0 37.0 37.0 37.0 40-44 36.069599999999994 37.0 37.0 37.0 37.0 37.0 45-49 36.05255 37.0 37.0 37.0 37.0 37.0 50-54 36.0251 37.0 37.0 37.0 37.0 37.0 55-59 36.00085 37.0 37.0 37.0 37.0 37.0 60-64 36.00595 37.0 37.0 37.0 37.0 37.0 65-69 35.8508 37.0 37.0 37.0 37.0 37.0 70-74 35.87765 37.0 37.0 37.0 37.0 37.0 75-79 35.890049999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.8986 37.0 37.0 37.0 37.0 37.0 85-89 35.793549999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.74405 37.0 37.0 37.0 37.0 37.0 95-99 35.77235 37.0 37.0 37.0 37.0 37.0 100-104 35.68575 37.0 37.0 37.0 37.0 37.0 105-109 35.782450000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.64375 37.0 37.0 37.0 37.0 37.0 115-119 35.61895 37.0 37.0 37.0 37.0 37.0 120-124 35.51845 37.0 37.0 37.0 37.0 37.0 125-129 35.52485 37.0 37.0 37.0 37.0 37.0 130-134 35.35295 37.0 37.0 37.0 37.0 37.0 135-139 35.3153 37.0 37.0 37.0 34.6 37.0 140-144 35.181149999999995 37.0 37.0 37.0 29.8 37.0 145-149 35.0298 37.0 37.0 37.0 27.4 37.0 150-151 34.770250000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 4.0 14 4.0 15 5.0 16 3.0 17 2.0 18 0.0 19 0.0 20 2.0 21 0.0 22 4.0 23 3.0 24 5.0 25 3.0 26 7.0 27 10.0 28 18.0 29 11.0 30 21.0 31 42.0 32 76.0 33 120.0 34 221.0 35 584.0 36 2713.0 37 141.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.519158527422995 21.738041572752316 12.046080641121963 26.69671925870273 2 26.1 26.825 29.175 17.9 3 20.200000000000003 26.75 31.324999999999996 21.725 4 21.425 34.0 25.374999999999996 19.2 5 25.275 35.85 22.325 16.55 6 20.150000000000002 39.25 22.45 18.15 7 20.349999999999998 23.075000000000003 37.3 19.275000000000002 8 20.8 27.025 26.75 25.424999999999997 9 21.6 27.825 28.549999999999997 22.025 10-14 23.71 29.535 25.990000000000002 20.765 15-19 23.34 28.194999999999997 27.700000000000003 20.765 20-24 23.178476771515726 28.369255388308247 26.944041606240937 21.50822623393509 25-29 23.112334250688015 28.546409807355516 27.080310232674503 21.260945709281962 30-34 23.138098334417045 29.330265592957534 27.134497073975893 20.397138998649528 35-39 23.513527029054355 28.61929289393409 28.074211131669752 19.7929689453418 40-44 23.964585834333736 28.661464585834334 26.840736294517807 20.533213285314126 45-49 22.760690172543136 28.052013003250813 27.94698674668667 21.240310077519382 50-54 23.96739673967397 28.987898789878987 27.077707770777078 19.966996699669966 55-59 24.048036027020263 26.8951713785339 27.760820615461597 21.295971978984237 60-64 23.283492523878582 27.904185627844175 28.059208881332196 20.753112966945043 65-69 22.85 28.005000000000003 27.889999999999997 21.255 70-74 23.937953465098825 28.181135851888918 27.05529146860145 20.82561921441081 75-79 23.60562253013856 27.68745935671052 28.34775649042069 20.35916162273023 80-84 23.494999999999997 28.605000000000004 27.065 20.835 85-89 24.045629659278532 27.97818582078351 26.977535398008705 20.998649121929255 90-94 23.393509026353954 27.799169875481322 27.649147372105816 21.15817372605891 95-99 22.23611180559028 28.471423571178562 28.801440072003597 20.49102455122756 100-104 23.407555666750063 28.586439829872408 27.215411558669 20.79059294470853 105-109 24.020613398709163 27.637964677040078 27.768049232000802 20.573372692249965 110-114 24.063609541431212 27.964194629194377 26.899034855228283 21.07316097414612 115-119 25.183851118114966 27.950372704987743 27.37005352944119 19.495722647456102 120-124 24.75356517388041 28.75656742556918 26.860145108831624 19.62972229171879 125-129 24.978738306068337 27.85532042623443 26.629646305468007 20.536294962229228 130-134 25.581744482810386 27.908722414051944 26.607616474002903 19.901916629134764 135-139 25.545436349079264 27.90232185748599 27.401921537229786 19.150320256204964 140-144 26.508976346451966 27.40911136670501 26.819022853428017 19.262889433415012 145-149 27.31685348278623 27.677141713370695 26.416132906325064 18.589871897518016 150-151 28.290790790790794 25.813313313313312 25.538038038038035 20.35785785785786 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.5 15 0.5 16 1.0 17 1.0 18 0.5 19 1.0 20 1.0 21 1.0 22 0.5 23 1.0 24 2.0 25 1.5 26 4.5 27 7.0 28 5.5 29 9.5 30 14.5 31 17.5 32 22.5 33 40.5 34 56.5 35 64.5 36 86.5 37 102.0 38 125.0 39 169.5 40 201.0 41 228.0 42 240.0 43 247.0 44 264.0 45 273.0 46 259.0 47 261.5 48 265.5 49 208.5 50 159.5 51 136.5 52 119.5 53 103.0 54 83.0 55 55.5 56 34.0 57 28.5 58 24.5 59 24.0 60 15.5 61 8.0 62 7.0 63 3.0 64 0.0 65 0.0 66 0.5 67 0.5 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.5 74 0.5 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.5 81 1.0 82 0.5 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17500000000000002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.015 25-29 0.075 30-34 0.034999999999999996 35-39 0.015 40-44 0.04 45-49 0.025 50-54 0.01 55-59 0.075 60-64 0.015 65-69 0.0 70-74 0.075 75-79 0.045 80-84 0.0 85-89 0.065 90-94 0.015 95-99 0.005 100-104 0.075 105-109 0.065 110-114 0.015 115-119 0.055 120-124 0.075 125-129 0.055 130-134 0.08499999999999999 135-139 0.08 140-144 0.015 145-149 0.08 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.85 #Duplication Level Percentage of deduplicated Percentage of total 1 69.28625664388763 45.625 2 18.337129840546698 24.15 3 7.517084282460136 14.85 4 2.9233105542900533 7.7 5 1.0630220197418374 3.5000000000000004 6 0.3416856492027335 1.35 7 0.30372057706909644 1.4000000000000001 8 0.07593014426727411 0.4 9 0.037965072133637055 0.22499999999999998 >10 0.11389521640091116 0.8 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGAACGGTGTATGCAAAGCCCTCAAATCCATGTGCGTAAATAAAAAGGTG 11 0.27499999999999997 No Hit GCCTATCAGGTATTACACTGGCACCTATAAATGATTCAACAAGATGCTTC 11 0.27499999999999997 No Hit GTGTACTATTCCATGTACGGGCATGTAGAGAAACTGGCAGAAGAGATTAG 10 0.25 No Hit ATATTTTAGGAGGAGTAAACATGGCAAGGAAGATCACCAAGCCACATGAC 9 0.22499999999999998 No Hit AGCACAAACGCTTCGGAAGAAAATCTTTTGGCCACAAAAATGGCTTCTGT 8 0.2 No Hit CATGGCACAAGCAATGGCATCGATGGCTGGTCTACGTGGAACATCTCAGG 8 0.2 No Hit CACAGAACTCTCTCTCACTCTCTCTCCCCTTCCGTGTCTCTGCTGGCTCC 7 0.17500000000000002 No Hit TGAAACCACATTGCAGAGTCCCCATAATACTGTTATGGCTGGCAAGTTAG 7 0.17500000000000002 No Hit GCCAACTGTGGGCCAGAAGCTGTCTGGTTCAAGACAGGAGCTCTACTTCT 7 0.17500000000000002 No Hit GGAATGTCTTTCAGTTTCTGCCAATGTTAATCTCTTTTCCTTGGTCATGT 7 0.17500000000000002 No Hit GGACAACATGTTGGACTTCCAAGACTTTTTGAGGCTAATGAAGAGGGAGG 7 0.17500000000000002 No Hit GAGGGCCGATCAGCATATGTAGCTCATCCTGCTCATGTTAAATTTGGCAC 7 0.17500000000000002 No Hit GGACCACTGGAGAACCTGGCTGACCACCTTTCTGACCCAGTAAACAACAA 7 0.17500000000000002 No Hit AAAAAAGGAAAACCAAAAACTTTACTCGCGAAACTTCTGTCCGCCAACAG 7 0.17500000000000002 No Hit GTTTGAAGCCCGAGCTGTTGCTCTCAAGGTTACCTGCTCCATCTGTAAGG 6 0.15 No Hit CACGTCTGCCCGACGTCTATTCAAACGCGGGTAGGATCCAAGCCAGGTTC 6 0.15 No Hit GGGGGAGTATCTGTTCATTGGAGCAGGTCTATTTGTGTTTATTTTCTGTG 6 0.15 No Hit GAATCTCAAAAGGCAACTAGAACTTGTCCTTGAAGAGAAGAATGAACTCG 6 0.15 No Hit GGGGCCCCTTCCCTTCCACCTTGAAACAGGGTATGTTGGAGTGGATGAAG 6 0.15 No Hit TCAAGATAACGAAAGAAAGAGGAGATGTTGAGGCTAAATCGATTGATAAA 6 0.15 No Hit CACTTCTGGTGGTTTGGTCAAAGACAAGGTTCTCTTTTGTAGCACAGAAA 6 0.15 No Hit CTTTAACCAAACATCCGTGCGATTCGTGCCACTCGTAGACGGCATCTCAC 6 0.15 No Hit GGCTCTGCCTACCAAACTAGGCAGCGGCAAGGCTCGAACACCACGTCCGG 6 0.15 No Hit GTTCTCCTTTGAAGAGACTGTGGCAGTGCTTCACTTGAAGCTGGAAAATA 5 0.125 No Hit CCTGTCTCAACTCGGATCAGTTCAATCAAAATAAGAACCTATCTCAACTT 5 0.125 No Hit GGTATGGCGTTGTAGGTCACTTGGAATCATGTGATGGAAATGAAAATTAT 5 0.125 No Hit CTCTCGCAATCGCTGCTTCTTTGTCTGTCTTTGGGTCGATCCGAAAGAGA 5 0.125 No Hit TGACTCCCGTATTGGAGTGCTGATAGAAGTAAACTGTGAAACTGACTTTG 5 0.125 No Hit GCATCTCAAACAATCACAAAACAACCCACCTCATCGTTGACCAGATGATA 5 0.125 No Hit AAAATGTCTCAAGGCTCTGCCTACCAAACTAGGCAGCGGCAAGGCTCGAA 5 0.125 No Hit AAGAAGCAAGACATCACCATTACTGGTGCTAGCACCTTACCTAGTGATGA 5 0.125 No Hit CCCATCTGTGATTTCAACGAACAAAGTCTTTGAGATCTGCCCACTTCGCT 5 0.125 No Hit GCTAGCTGAGAGGGATGGAACAATTGAAGAGGTGGCTGTGACAAGGAATG 5 0.125 No Hit GCAAAGTAGAGGGAGGTTCCTCTGAGTACATAGAATTTGGTGACTGTAAA 5 0.125 No Hit GCGAAATCGGAGAACAAGATGTGTTTTGATTGTAATGCCAAAAATCCTAC 5 0.125 No Hit TGGTGGATTGGAGTTGGATTGTTTGTGAAATAAAGTTAAGTTATTGTGTT 5 0.125 No Hit GGTGAATCGGTTGGAATGGAGACAGAAGGTTTTGCTATCTATGATTCAAT 5 0.125 No Hit GTTTGTTGAGATGTTTGTCGGTGTTGGAGCCTCTAGAGTCCGGGATCTCT 5 0.125 No Hit GATAGGGTGAAAAAGGTAGACTTGTTTGAGAATGGAACCATTGCTATCGT 5 0.125 No Hit ATTTTAAGCAAAACACACAACCAAACCACTTCGCAGCTCCTCCTCTTTCA 5 0.125 No Hit CTTGCATCCTAAGGATGGTGTCTACCCTGAGAAGGTGAACCCAGGGCGCC 5 0.125 No Hit GGCAAGGATGGGGTTGTCCCACTCTCAAATCAGTCCAGCTTTCATGATAA 5 0.125 No Hit GGCTCACTGGAGTTACCTGGCAAGATGCTGGAAAGGTGGAATTAGTTGAA 5 0.125 No Hit CCATATTCAACAGCATTATATCAGTTGCTGGTAATATAAATTATTATGAC 5 0.125 No Hit ATTTCATATGAGCAGTTCATTAGGGAAAGCAAAGGAATCATTGAATCATT 5 0.125 No Hit ATGGAACTGGTGGTGGCATGAACCTCAGGGATGGGTTAGATGCATCTGGA 5 0.125 No Hit GCCATTTGAACTTGACAGTTATAGTAATGCTTCTGAACCTCGTTTAAGGC 5 0.125 No Hit CAAAGAGAAAAGGATCTTCGCGAAGGGCTGCAATTATACAAAAATGCCAA 5 0.125 No Hit CCACATACCAAGTTTGAGGCGATTGTTTATGTTTTGAAGAAGGAAGAAGG 5 0.125 No Hit ATACCGTGGAGTATCAGAAGGTAGTGAAACAAGACATTCGACCTGCACTC 5 0.125 No Hit AGGAGATAAATGGAAGTCACTGTCAGAGGCTGAGAAAGCACCCTTTGTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.1875 0.0 0.0 0.0 0.0 86-87 0.3125 0.0 0.0 0.0 0.0 88-89 0.35 0.0 0.0 0.0 0.0 90-91 0.4 0.0 0.0 0.0 0.0 92-93 0.45 0.0 0.0 0.0 0.0 94-95 0.575 0.0 0.0 0.0 0.0 96-97 0.725 0.0 0.0 0.0 0.0 98-99 0.9375 0.0 0.0 0.0 0.0 100-101 1.2 0.0 0.0 0.0 0.0 102-103 1.375 0.0 0.0 0.0 0.0 104-105 1.525 0.0 0.0 0.0 0.0 106-107 1.65 0.0 0.0 0.0 0.0 108-109 1.9125 0.0 0.0 0.0 0.0 110-111 2.3 0.0 0.0 0.0 0.0 112-113 2.7 0.0 0.0 0.0 0.0 114-115 3.175 0.0 0.0 0.0 0.0 116-117 3.5875000000000004 0.0 0.0 0.0 0.0 118-119 4.175 0.0 0.0 0.0 0.0 120-121 4.775 0.0 0.0 0.0 0.0 122-123 5.725 0.0 0.0 0.0 0.0 124-125 6.2375 0.0 0.0 0.0 0.0 126-127 6.9125 0.0 0.0 0.0 0.0 128-129 7.6625 0.0 0.0 0.0 0.0 130-131 8.2375 0.0 0.0 0.0 0.0 132-133 8.9875 0.0 0.0 0.0 0.0 134-135 9.7 0.0 0.0 0.0 0.0 136-137 10.475000000000001 0.0 0.0 0.0 0.0 138-139 11.1875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTGAA 10 0.006830828 145.0 1 ACAGAAC 10 0.006830828 145.0 2 GATGTAG 10 0.006830828 145.0 2 AACTCTC 10 0.006830828 145.0 6 TGTAGCA 10 0.006830828 145.0 4 AGATGAT 10 0.006830828 145.0 145 ATGTAGC 10 0.006830828 145.0 3 GTAGCAG 10 0.006830828 145.0 5 >>END_MODULE Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745400 spots for SRR13695480.sra Written 745400 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra Read 745383 spots for SRR13695480.sra Written 745383 spots for SRR13695480.sra SRR ids: ['SRR13695480.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ebuno8nt SRR13695480.sra spots: 14907677 blocks: [[1, 745383], [745384, 1490766], [1490767, 2236149], [2236150, 2981532], [2981533, 3726915], [3726916, 4472298], [4472299, 5217681], [5217682, 5963064], [5963065, 6708447], [6708448, 7453830], [7453831, 8199213], [8199214, 8944596], [8944597, 9689979], [9689980, 10435362], [10435363, 11180745], [11180746, 11926128], [11926129, 12671511], [12671512, 13416894], [13416895, 14162277], [14162278, 14907677]] SRR13695480 file size 5044580 SRR13695480 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695480 SRR13695480_1.fastq SRR13695480_2.fastq Input file: SRR13695480_1.fastq Paired file: SRR13695480_2.fastq trimmed: SRR13695480-trimmed-pair1.fastq, SRR13695480-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 06:15:43 2025 >> started Wed Feb 12 06:16:01 2025 >> done (18.281s) 14907677 read pairs processed; of these: 112 ( 0.00%) short read pairs filtered out after trimming by size control 1738 ( 0.01%) empty read pairs filtered out after trimming by size control 14905827 (99.99%) read pairs available; of these: 2326993 (15.61%) trimmed read pairs available after processing 12578834 (84.39%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 0 0.00% 20 1 0.00% 21 0 0.00% 22 0 0.00% 23 2 0.00% 24 2 0.00% 25 1 0.00% 26 2 0.00% 27 3 0.00% 28 1 0.00% 29 4 0.00% 30 6 0.00% 31 0 0.00% 32 3 0.00% 33 4 0.00% 34 3 0.00% 35 4 0.00% 36 3 0.00% 37 8 0.00% 38 7 0.00% 39 8 0.00% 40 6 0.00% 41 6 0.00% 42 8 0.00% 43 16 0.00% 44 8 0.00% 45 17 0.00% 46 14 0.00% 47 25 0.00% 48 24 0.00% 49 20 0.00% 50 36 0.00% 51 53 0.00% 52 58 0.00% 53 52 0.00% 54 58 0.00% 55 76 0.00% 56 81 0.00% 57 126 0.00% 58 122 0.00% 59 124 0.00% 60 156 0.00% 61 223 0.00% 62 250 0.00% 63 287 0.00% 64 325 0.00% 65 314 0.00% 66 431 0.00% 67 515 0.00% 68 544 0.00% 69 710 0.00% 70 833 0.01% 71 965 0.01% 72 1189 0.01% 73 1257 0.01% 74 1501 0.01% 75 1695 0.01% 76 1878 0.01% 77 2101 0.01% 78 2409 0.02% 79 2830 0.02% 80 3082 0.02% 81 3725 0.02% 82 4072 0.03% 83 4716 0.03% 84 5184 0.03% 85 6051 0.04% 86 6343 0.04% 87 6583 0.04% 88 7700 0.05% 89 7923 0.05% 90 8851 0.06% 91 9733 0.07% 92 10242 0.07% 93 11449 0.08% 94 12444 0.08% 95 13266 0.09% 96 14227 0.10% 97 15301 0.10% 98 16060 0.11% 99 16468 0.11% 100 17645 0.12% 101 18454 0.12% 102 19713 0.13% 103 20795 0.14% 104 21548 0.14% 105 22801 0.15% 106 24411 0.16% 107 25165 0.17% 108 26038 0.17% 109 27345 0.18% 110 27701 0.19% 111 28903 0.19% 112 29517 0.20% 113 30998 0.21% 114 32175 0.22% 115 33093 0.22% 116 34127 0.23% 117 35492 0.24% 118 36887 0.25% 119 37091 0.25% 120 38319 0.26% 121 39393 0.26% 122 39985 0.27% 123 40567 0.27% 124 41932 0.28% 125 42557 0.29% 126 44136 0.30% 127 45091 0.30% 128 45166 0.30% 129 46605 0.31% 130 47720 0.32% 131 47886 0.32% 132 48234 0.32% 133 50410 0.34% 134 50126 0.34% 135 50871 0.34% 136 52546 0.35% 137 52636 0.35% 138 53434 0.36% 139 55126 0.37% 140 54765 0.37% 141 55513 0.37% 142 56266 0.38% 143 56676 0.38% 144 57620 0.39% 145 58587 0.39% 146 57993 0.39% 147 59607 0.40% 148 60037 0.40% 149 60878 0.41% 150 61586 0.41% 151 12578834 84.39% 14905827 reads passed initial QC criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=2.61 fanout-score-rank=11 prefix-density=0.41 prefix-fanout=2.4 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.02 sequence-density-rank=25 fanout-score=36.96 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=5.4 sequence=TTCAACAACTTCCCTATCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAATATGACAAGGAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATGGAGCTATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATTATTTTTAGAAATCCCATACTGCCACCGAATATTCAGTCCTTTAAGAAATCGAACAGCATACCCAACATAGTAAAAACCATCAATAATGCAAATACCGTTACCACAAGTGCAAATACTCCCATTCCTACCTCTCCA criterion=sequence-density sequence-density=0.94 sequence-density-rank=1 fanout-score=2.17 fanout-score-rank=27 prefix-density=0.96 prefix-fanout=2.1 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=45.16 fanout-score-rank=1 prefix-density=0.06 prefix-fanout=4.3 sequence=TGGCCATGTAAAACACAATATTTTTCAGTATGAAAGCTTTAGTGATAGCTATTCTTATAGCTACCATTGCCTTCTCTCCCTTATCCATGGCAGCTCGAGAATTGGTCGACTATGGAAAAGATAGCGTTACCGTCAATATCCCATCAACTGGCGATGTATCATCTAGAAGCCAGCCTCCTACCTATGCCCCACGAACTGGCAGTGGAT SRR13695480 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 06:16:44 Started mapping on | Feb 12 06:16:44 Finished on | Feb 12 06:18:57 Mapping speed, Million of reads per hour | 403.47 Number of input reads | 14905827 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 13732521 Uniquely mapped reads % | 92.13% Average mapped length | 292.83 Number of splices: Total | 13302425 Number of splices: Annotated (sjdb) | 12997362 Number of splices: GT/AG | 13031179 Number of splices: GC/AG | 207700 Number of splices: AT/AC | 8348 Number of splices: Non-canonical | 55198 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.83 Insertion rate per base | 0.02% Insertion average length | 2.37 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 361917 % of reads mapped to multiple loci | 2.43% Number of reads mapped to too many loci | 38964 % of reads mapped to too many loci | 0.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.01% % of reads unmapped: other | 0.18% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 811580 811580 811580 N_multimapping 361917 361917 361917 N_noFeature 476956 13390365 669713 N_ambiguous 234579 1408 84412 UnstrandedReadsAssigned:13020986 PositiveStrandReadsAssigned:340748 NegativeStrandReadsAssigned:12978396 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695480 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695480-trimmed-pair1.fastq SRR13695480-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 14,905,827 reads, 13,051,641 reads pseudoaligned [quant] estimated average fragment length: 231.902 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,174 rounds 52401 SRR13695480.ke.tsv 34699 SRR13695480.se.tsv 87100 total ==> SRR13695480.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1787.1 630 22.0823 Potri.005G024800.1.v4.1 1035 804.098 533 41.5212 Potri.004G059700.1.v4.1 961 730.149 6 0.514744 Potri.007G009000.2.v4.1 1416 1185.1 0 0 Potri.003G141000.2.v4.1 2943 2712.1 712.416 16.4543 Potri.016G087400.1.v4.1 270 90.6669 766 529.214 Potri.015G069301.1.v4.1 564 339.257 0 0 Potri.010G195200.1.v4.1 1773 1542.1 74 3.00588 Potri.012G127500.1.v4.1 977 746.121 83 6.9682 ==> SRR13695480.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 162 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 206 Potri.001G212900.v4.1 340 Potri.001G182400.v4.1 2 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 3 SRR13695480 completed mapping pipeline successfully