Starting /dee2/code/volunteer_pipeline.sh SRR13695481 current disk space = 3050309357568 free memory = 1582741648 SRR13695481 SRAfilesize 21c23c03f549f43dac28d3b105afd37f SRR13695481.sra SRR13695481.sra file validated SRR13695481 is paired end SRR13695481 is conventional basespace SRR13695481 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695481_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.53 37.0 37.0 37.0 37.0 37.0 2 36.25475 37.0 37.0 37.0 37.0 37.0 3 36.5065 37.0 37.0 37.0 37.0 37.0 4 36.493 37.0 37.0 37.0 37.0 37.0 5 36.558 37.0 37.0 37.0 37.0 37.0 6 36.4815 37.0 37.0 37.0 37.0 37.0 7 36.4205 37.0 37.0 37.0 37.0 37.0 8 36.5425 37.0 37.0 37.0 37.0 37.0 9 36.54 37.0 37.0 37.0 37.0 37.0 10-14 36.589800000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.4874 37.0 37.0 37.0 37.0 37.0 20-24 36.5131 37.0 37.0 37.0 37.0 37.0 25-29 36.463699999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.4507 37.0 37.0 37.0 37.0 37.0 35-39 36.4208 37.0 37.0 37.0 37.0 37.0 40-44 36.3637 37.0 37.0 37.0 37.0 37.0 45-49 36.4119 37.0 37.0 37.0 37.0 37.0 50-54 36.37769999999999 37.0 37.0 37.0 37.0 37.0 55-59 36.3957 37.0 37.0 37.0 37.0 37.0 60-64 36.32940000000001 37.0 37.0 37.0 37.0 37.0 65-69 36.2256 37.0 37.0 37.0 37.0 37.0 70-74 36.3048 37.0 37.0 37.0 37.0 37.0 75-79 36.2696 37.0 37.0 37.0 37.0 37.0 80-84 36.236599999999996 37.0 37.0 37.0 37.0 37.0 85-89 36.2327 37.0 37.0 37.0 37.0 37.0 90-94 36.198299999999996 37.0 37.0 37.0 37.0 37.0 95-99 36.113299999999995 37.0 37.0 37.0 37.0 37.0 100-104 36.1361 37.0 37.0 37.0 37.0 37.0 105-109 36.0763 37.0 37.0 37.0 37.0 37.0 110-114 36.1044 37.0 37.0 37.0 37.0 37.0 115-119 36.0498 37.0 37.0 37.0 37.0 37.0 120-124 35.9633 37.0 37.0 37.0 37.0 37.0 125-129 35.9152 37.0 37.0 37.0 37.0 37.0 130-134 35.987 37.0 37.0 37.0 37.0 37.0 135-139 35.9565 37.0 37.0 37.0 37.0 37.0 140-144 35.7589 37.0 37.0 37.0 37.0 37.0 145-149 35.6428 37.0 37.0 37.0 37.0 37.0 150-151 35.397999999999996 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 0.0 25 4.0 26 9.0 27 10.0 28 14.0 29 19.0 30 31.0 31 35.0 32 38.0 33 84.0 34 123.0 35 314.0 36 2975.0 37 343.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.900000000000002 11.200000000000001 7.6499999999999995 49.25 2 17.759356945491085 15.146948003014318 40.59281587540819 26.50087917608641 3 17.275 19.400000000000002 27.375 35.949999999999996 4 23.849999999999998 26.224999999999998 22.2 27.725 5 22.725 32.85 25.2 19.225 6 19.75 36.425000000000004 22.725 21.099999999999998 7 13.625000000000002 27.375 42.225 16.775000000000002 8 16.7 22.900000000000002 37.0 23.400000000000002 9 19.0 22.225 35.55 23.225 10-14 20.599999999999998 29.64 27.139999999999997 22.62 15-19 20.13 27.725 28.315 23.830000000000002 20-24 20.075000000000003 28.73 27.310000000000002 23.885 25-29 19.814999999999998 27.915 28.425 23.845 30-34 20.474999999999998 28.205000000000002 27.83 23.49 35-39 20.19 28.610000000000003 27.27 23.93 40-44 19.79 28.215 28.055000000000003 23.94 45-49 19.765 28.675 28.000000000000004 23.56 50-54 19.615 29.044999999999998 27.74 23.599999999999998 55-59 19.400000000000002 28.549999999999997 28.035 24.015 60-64 20.630000000000003 28.205000000000002 27.900000000000002 23.265 65-69 20.575 27.650000000000002 27.54 24.235 70-74 19.62 27.41 29.005 23.965 75-79 19.54 28.285 28.225 23.95 80-84 20.665 27.195000000000004 28.294999999999998 23.845 85-89 20.13 27.655 28.08 24.135 90-94 20.365 29.060000000000002 26.91 23.665 95-99 20.41 28.389999999999997 28.749999999999996 22.45 100-104 20.75 27.92 27.96 23.369999999999997 105-109 20.835 28.715000000000003 27.3 23.150000000000002 110-114 21.0 28.665000000000003 27.88 22.455 115-119 20.979999999999997 27.889999999999997 27.839999999999996 23.29 120-124 20.5 28.595 27.529999999999998 23.375 125-129 20.5 27.54 28.000000000000004 23.96 130-134 20.825 28.395 27.21 23.57 135-139 21.085 28.9 26.790000000000003 23.225 140-144 20.835 28.71 27.04 23.415 145-149 20.415 28.73 27.07 23.785 150-151 19.85 27.800000000000004 27.625 24.725 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 1.0 20 1.0 21 0.0 22 0.5 23 1.5 24 1.5 25 2.5 26 4.5 27 7.5 28 12.0 29 15.0 30 17.5 31 22.5 32 27.0 33 39.5 34 53.0 35 75.0 36 88.0 37 92.5 38 122.5 39 154.5 40 212.5 41 240.0 42 236.5 43 253.5 44 255.5 45 276.5 46 261.5 47 262.0 48 260.0 49 198.5 50 172.5 51 157.0 52 120.5 53 90.5 54 65.0 55 45.5 56 45.0 57 31.0 58 19.0 59 19.0 60 17.0 61 9.5 62 3.5 63 1.5 64 2.0 65 2.0 66 0.5 67 1.5 68 1.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.475 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.22500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 70.42872847196774 48.05 2 19.237816049835104 26.25 3 6.339318431659949 12.975 4 2.1619640894100405 5.8999999999999995 5 1.282521069989007 4.375 6 0.3297911322828875 1.35 7 0.14657383657017223 0.7000000000000001 8 0.07328691828508611 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTCCTTCACCCAAAAGACAATCTGCCCTGAAGTTCTTTGTTGCATTTG 8 0.2 No Hit CCTGTTTTAAAGCGCTCTTTTGAAAGAGTGAACCATCCTTCTCCAGGTAC 8 0.2 No Hit GTCCAGTAGCAAAAACAGACGCAATGCTTCCTGGGTTGTTTTTTCTAATC 7 0.17500000000000002 No Hit CATCATATTTGCCAAAATCAACTTCATCGAATGTTGCATTGAGAGTAAAA 7 0.17500000000000002 No Hit CTCATATTCACCGCATTATGCTACCATCTCTTATCTTAACGGTAACATTA 7 0.17500000000000002 No Hit CTGGCCTTTGATTCCATGTTTGTGGCAAGTGCCATAGCTAGTGCTTAGGT 7 0.17500000000000002 No Hit AATGAATTGGCGGTGAAACTCAAGCTGCTGCGAGTAAAATAGGTTGCAAT 6 0.15 No Hit GCCACACGACCCCATTTTTCAAGACGCCCAGAAGAAGTGGGTTTCTCGAA 6 0.15 No Hit TTGAAGTCTGTGCAATTCTCATCAAATAAAAATTTGAAAGGTGTATCTCC 6 0.15 No Hit GCTTCCTTTAACACCAGGACGAGGTTTGGAAGAATCAGCAACATGAACAG 6 0.15 No Hit CGTCCATTTCCTGCCTCTGAACCCTTCCCAGCAGCTCAATCTGCAAAATC 6 0.15 No Hit TCATGTTGGAACGAGCTGCAATTGTGTTCATGTCCATGACACCCATTCCC 6 0.15 No Hit TGCTGGTGGAGAATAATCCTGATCAGTGTTTAATGAACTGCTGATCCTAT 6 0.15 No Hit CTTCAGTGCCTGAAAGTTTCATATCTTCTTTCCTGAATTTCTCAAGAGTA 6 0.15 No Hit TGTACACGGTATCTCCAGTAATGTTTTGGATTTGTTGGGTCATTGATCGT 6 0.15 No Hit GGCTAGTTCAGGAATCTCTGGTAATATTAGGCTTGATGGACTTCCGAAAA 5 0.125 No Hit CCCACAACTTCGTTTTTGTACTGAATAGATCTATCCTTGCCAGTGCTTTT 5 0.125 No Hit GTGAGAAGAAGGGGGTTTAAATAAGGGAGAATTGGAGGAGGGAGTGAGGA 5 0.125 No Hit CCCGGGGAAAATGTCGCATAAAGGGCAGCAGCAGCCTGAGATTTCCCATC 5 0.125 No Hit GCTCCCTTCATACTCAGCAGAAAGCTCATAGCACTCTTGGACCGTTTCCT 5 0.125 No Hit CTCTTTTAGTGTTTCACTTGCTATATTCTCGCCCTCATCTTTGGTCGTCT 5 0.125 No Hit CAAGAGACAAAAAAGATGAAGACTATGGGGGAAAGGAGAGATGAGACTAA 5 0.125 No Hit CCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGTTGTCGCAGGT 5 0.125 No Hit ACCAAACATCCACCATCAACCCTTCCACACCTGCACTCTTCAACGCCTGC 5 0.125 No Hit GCCATAATTCTTTGCTTGACATATTTTACCTTCATGACTTGGTCCTTCCA 5 0.125 No Hit GATAAAACAGCGTGAAGATTAGAGTAACAAAATTGAAATCCTCATCAACT 5 0.125 No Hit GTAGCATGGAAGGAAGATATGATTAGCTGCCACTGCCAGCCTCTGAAATG 5 0.125 No Hit CTGGGGATATAGAATGACCTCGCTATCCTCTCCTGGTGCTTGTCCAGTAC 5 0.125 No Hit CCTTCCTGAAGTTCCAGCTTGTAAGCCCTTCGTTGCTCAATAGTTGAAAC 5 0.125 No Hit TCTCACTTTCCGGGGGCGAAGTTTGTGGCATATGCCCAGGCGTTGTTGTT 5 0.125 No Hit GTTCTGGTAAAAGTTATCAAGCTCGTAATAGATGAAAACAGGACTTTTCA 5 0.125 No Hit CAACAAAAATATAGGGGCACACCAAGACCTTCTCCACAGGGACCAAAACC 5 0.125 No Hit ATTAAAACCACACCAGAGGCCACAGACATGGCCAATACATAACAATGAAG 5 0.125 No Hit ATCGTAGCACTGAGGAGAGCAAAGTTGTTGTGCGAAGCGAGACTCAAGGA 5 0.125 No Hit CTCTTCATCTCCTTGTATCCAGTACTATTCATCATTTCTGTCTCAGGCAC 5 0.125 No Hit CACGACTCGGCATCACCAACAGCACAAAAGCGATCAAAAGCATCGGGATC 5 0.125 No Hit CCGCCATTCAAGGCCAAGAGCAAGTTTAGGCTGTTAATTCTTCTCCGCTT 5 0.125 No Hit CACATATTCTGCCTTTTTCTTCTCAAGTTTTTCCTCAATCTTCTTCAACT 5 0.125 No Hit CACCAGCGAGGCCAACAGATTTGAGCATCACTGGGAGTGTATACGTCACA 5 0.125 No Hit TCTGTATCTAGGATGTGGACATCTGACAAATAATAATCATGCCCATCTTC 5 0.125 No Hit CAGCCTTTTTGGCAGCCTCTATTGCTTTAGGTGGCAATCGTATTTGAGGA 5 0.125 No Hit CTTGCAATCCTGTTGAGTTAACCTTCTCCTGTGTGCCAAGTAGCTAAGAA 5 0.125 No Hit CGGCGCTAGTTTCTAAGTAAACATCAACGGAGGATTTTACAAGACATAAT 5 0.125 No Hit TCTCACTCTGTTACTAATGACACCAGAGTTCCTCTCAGCTCTGATAATTT 5 0.125 No Hit GTAAAAAACTAAATGCAGAACTGCTTCAGAACAATGGAGAGAATGCCAGC 5 0.125 No Hit GCTCATCTTCGCACTTGTATATCTTCGCGTTTGCATATCCGAGCTTTATT 5 0.125 No Hit ACAGGAACCACACCATATGTAATAAGATGAGCAAATGGCCAAAGCTTCCA 5 0.125 No Hit CGGTTACAAAAGAAAAAAAGAAGAAGAAAGAAATGCAGACGTACAACCAA 5 0.125 No Hit ATTATTTGTCTAGCAGGTTCTGAGAGTGAAGGGAGTATGCTTGAAGGTGA 5 0.125 No Hit GGCCTGTTTAACACCTCCAGTGTCGTTGATGGCAATGACGTCAAGAGGTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.1125 0.0 0.0 0.0 0.0 84-85 0.15 0.0 0.0 0.0 0.0 86-87 0.15 0.0 0.0 0.0 0.0 88-89 0.16249999999999998 0.0 0.0 0.0 0.0 90-91 0.1875 0.0 0.0 0.0 0.0 92-93 0.2875 0.0 0.0 0.0 0.0 94-95 0.4125 0.0 0.0 0.0 0.0 96-97 0.5 0.0 0.0 0.0 0.0 98-99 0.6499999999999999 0.0 0.0 0.0 0.0 100-101 0.8125 0.0 0.0 0.0 0.0 102-103 1.0 0.0 0.0 0.0 0.0 104-105 1.075 0.0 0.0 0.0 0.0 106-107 1.3 0.0 0.0 0.0 0.0 108-109 1.6124999999999998 0.0 0.0 0.0 0.0 110-111 1.7000000000000002 0.0 0.0 0.0 0.0 112-113 1.8125 0.0 0.0 0.0 0.0 114-115 2.1 0.0 0.0 0.0 0.0 116-117 2.3 0.0 0.0 0.0 0.0 118-119 2.5875 0.0 0.0 0.0 0.0 120-121 3.125 0.0 0.0 0.0 0.0 122-123 3.7 0.0 0.0 0.0 0.0 124-125 4.0875 0.0 0.0 0.0 0.0 126-127 4.3625 0.0 0.0 0.0 0.0 128-129 4.9375 0.0 0.0 0.0 0.0 130-131 5.65 0.0 0.0 0.0 0.0 132-133 6.0875 0.0 0.0 0.0 0.0 134-135 6.65 0.0 0.0 0.0 0.0 136-137 7.5375 0.0 0.0 0.0 0.0 138-139 8.0375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCTA 10 0.006830828 145.0 7 TATATAA 10 0.006830828 145.0 145 CAGAGAC 10 0.006830828 145.0 2 AGAGACT 10 0.006830828 145.0 3 CTAAATG 10 0.006830828 145.0 9 GACTGCT 10 0.006830828 145.0 6 TGCTAGG 10 0.006830828 145.0 9 CTGCTAG 10 0.006830828 145.0 8 AAAAACT 10 0.006830828 145.0 4 GAGACTG 10 0.006830828 145.0 4 GTAAAAA 10 0.006830828 145.0 1 >>END_MODULE SRR13695481 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695481_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1805 37.0 37.0 37.0 37.0 37.0 2 36.055 37.0 37.0 37.0 37.0 37.0 3 35.9475 37.0 37.0 37.0 37.0 37.0 4 36.0625 37.0 37.0 37.0 37.0 37.0 5 36.2325 37.0 37.0 37.0 37.0 37.0 6 36.193 37.0 37.0 37.0 37.0 37.0 7 36.0845 37.0 37.0 37.0 37.0 37.0 8 36.2685 37.0 37.0 37.0 37.0 37.0 9 36.153 37.0 37.0 37.0 37.0 37.0 10-14 36.2738 37.0 37.0 37.0 37.0 37.0 15-19 36.2273 37.0 37.0 37.0 37.0 37.0 20-24 36.222899999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.1682 37.0 37.0 37.0 37.0 37.0 30-34 36.1012 37.0 37.0 37.0 37.0 37.0 35-39 36.0829 37.0 37.0 37.0 37.0 37.0 40-44 36.04299999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.042500000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.025400000000005 37.0 37.0 37.0 37.0 37.0 55-59 35.9821 37.0 37.0 37.0 37.0 37.0 60-64 35.9524 37.0 37.0 37.0 37.0 37.0 65-69 35.8556 37.0 37.0 37.0 37.0 37.0 70-74 35.7593 37.0 37.0 37.0 37.0 37.0 75-79 35.763000000000005 37.0 37.0 37.0 37.0 37.0 80-84 35.87179999999999 37.0 37.0 37.0 37.0 37.0 85-89 35.7236 37.0 37.0 37.0 37.0 37.0 90-94 35.7392 37.0 37.0 37.0 37.0 37.0 95-99 35.767700000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.6943 37.0 37.0 37.0 37.0 37.0 105-109 35.661199999999994 37.0 37.0 37.0 37.0 37.0 110-114 35.5951 37.0 37.0 37.0 37.0 37.0 115-119 35.5995 37.0 37.0 37.0 37.0 37.0 120-124 35.562 37.0 37.0 37.0 37.0 37.0 125-129 35.5279 37.0 37.0 37.0 37.0 37.0 130-134 35.44709999999999 37.0 37.0 37.0 34.6 37.0 135-139 35.43470000000001 37.0 37.0 37.0 34.6 37.0 140-144 35.3817 37.0 37.0 37.0 37.0 37.0 145-149 35.2368 37.0 37.0 37.0 29.8 37.0 150-151 34.93675 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 0.0 13 0.0 14 0.0 15 2.0 16 0.0 17 0.0 18 1.0 19 2.0 20 2.0 21 1.0 22 4.0 23 4.0 24 7.0 25 5.0 26 9.0 27 19.0 28 16.0 29 18.0 30 30.0 31 49.0 32 60.0 33 104.0 34 236.0 35 631.0 36 2634.0 37 165.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.80220330495744 19.45418127190786 12.193289934902353 33.550325488232346 2 26.5 26.025 32.35 15.125 3 20.175 28.7 32.525 18.6 4 24.4 32.475 24.25 18.875 5 24.125 38.05 22.025 15.8 6 17.5 40.949999999999996 23.175 18.375 7 19.950000000000003 21.575 39.65 18.825 8 19.975 25.0 31.175000000000004 23.849999999999998 9 22.25 23.575 32.875 21.3 10-14 22.425 29.465000000000003 27.33 20.78 15-19 23.095 27.615000000000002 27.99 21.3 20-24 22.770000000000003 27.87 28.310000000000002 21.05 25-29 22.52 28.084999999999997 28.970000000000002 20.424999999999997 30-34 21.8 28.185 28.82 21.195 35-39 22.57 28.16 28.299999999999997 20.97 40-44 22.36 28.225 28.735 20.68 45-49 22.39 28.849999999999998 27.67 21.09 50-54 22.59 28.389999999999997 27.92 21.099999999999998 55-59 22.475 28.744999999999997 26.93 21.85 60-64 22.955000000000002 28.945 27.77 20.330000000000002 65-69 23.119999999999997 28.095 27.644999999999996 21.14 70-74 22.285 29.23 27.58 20.905 75-79 22.925 28.084999999999997 28.075 20.915 80-84 23.355 28.9 27.544999999999998 20.200000000000003 85-89 22.335 28.49 27.639999999999997 21.535 90-94 22.205 28.52 28.439999999999998 20.835 95-99 23.380000000000003 28.244999999999997 27.445000000000004 20.93 100-104 24.22 27.825 27.060000000000002 20.895 105-109 22.759999999999998 28.215 29.065 19.96 110-114 23.44 27.944999999999997 28.185 20.43 115-119 23.75 29.325000000000003 26.555 20.369999999999997 120-124 24.675 27.71 27.185 20.43 125-129 24.98 28.435 26.58 20.005 130-134 24.87 27.500000000000004 27.54 20.09 135-139 25.430000000000003 28.83 26.200000000000003 19.54 140-144 25.44 28.470000000000002 26.450000000000003 19.64 145-149 25.06 27.215 27.615000000000002 20.11 150-151 24.675 28.1625 27.3375 19.825 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.0 4 0.5 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.5 16 2.0 17 1.0 18 0.5 19 1.0 20 1.0 21 1.5 22 1.5 23 3.5 24 5.0 25 4.0 26 4.5 27 7.5 28 11.0 29 13.0 30 17.0 31 20.0 32 26.0 33 38.0 34 46.5 35 72.5 36 100.0 37 112.5 38 148.5 39 186.5 40 215.0 41 264.5 42 265.5 43 259.5 44 283.0 45 273.0 46 259.5 47 240.0 48 227.0 49 189.0 50 140.5 51 128.5 52 112.0 53 75.0 54 54.0 55 48.5 56 34.0 57 23.0 58 18.5 59 13.0 60 12.0 61 10.5 62 3.0 63 6.0 64 6.0 65 1.5 66 0.5 67 0.5 68 0.5 69 0.0 70 0.0 71 0.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.325 #Duplication Level Percentage of deduplicated Percentage of total 1 71.16721551408708 48.625 2 18.294914013904133 25.0 3 6.54957921697768 13.425 4 2.0856201975850714 5.7 5 1.2806439809732895 4.375 6 0.3293084522502744 1.35 7 0.14635931211123307 0.7000000000000001 8 0.10976948408342481 0.6 9 0.03658982802780827 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTCGATTAACAGCTGGTGTCTCACCTCTGTCTCTGCCTCTAAGAGATCA 9 0.22499999999999998 No Hit AATTGTTGTTAATATTGGTTTTCAAGTCACATCTGTTGTTCCAATTTTAC 8 0.2 No Hit GAAAATTCCTGCTTTTGTCAGCCTGTTTTGTTTCCATAATACCATTATGG 8 0.2 No Hit GTTTGGTCTGCTTCCGCCCCCCCTCTCTCTCTGCGAGGTAATACTCGTTG 8 0.2 No Hit GCATCAGTAGGTTGGGTAGCTAGGTCTGTAGAAGCACGCATTCGAGACAA 7 0.17500000000000002 No Hit CATAACCAATCTGAACGAAAAAGGAGAGATAAGATAAACCAGAGAATGAA 7 0.17500000000000002 No Hit CAGTAACAAGAACGTTGTGTACGAGATAGAGGGATCGTATGCACAAAGAT 7 0.17500000000000002 No Hit ACAATATTTTCTGTTATCTTTAATGGCTTCTATGATTTCTATCCTAAATA 7 0.17500000000000002 No Hit GGAAGATGAAGAAAGAAGATGCCGATATTTCAAGAACTTGGTGGGGCCTG 6 0.15 No Hit TGGGTGAAGATGACGAGGATGAAGATGAAGCAGATGCTGTAAATACTTCA 6 0.15 No Hit CTCCATTTTCAAGCAATTGCAGAGGTTGAGATTTATAAATATGCTCCATG 6 0.15 No Hit TAAATTAAAGCGAAGCAATTATGACAGGGAAGGCTAAGCCAAAGAAGCAC 6 0.15 No Hit CTTACTCTTGCCAAACCCGAAGGAGGTTTAGATTCCACTTCTGCAACCAA 6 0.15 No Hit TTTTGATTGTAAACCAAAGTGATTATAAATTACCCCCACCATGAGAGCCT 6 0.15 No Hit CGGCAGATGAAGATACTATCAATAGCCCTTCTTCGGTTCTTCCGCTTCAA 6 0.15 No Hit ACGAGGCAGAAGATCAACCTCCTGCAAAAAGGCGACTTTTATCTGCTGTT 6 0.15 No Hit AAATATTATGCTCAGCTGGCTCCTCTTCAGTACTTAGGCCAAGCACAGTT 6 0.15 No Hit GCTTGGGTCACTGGTCGGGATGGTTAATCTGAGTTGATCCAGGTTGTTGG 5 0.125 No Hit CAATGGGAGACTGGATGAATAAACAATTGCGCATTCCCGATCCTCATTCT 5 0.125 No Hit GGGAGGACAATGTACACCATCCGCCAAAGAATTGGCCCATTATTCATGAA 5 0.125 No Hit AAGCAAATCCATCTGAGCATCCGTTTTCGCCTTCTGCACAGTGAAATGAA 5 0.125 No Hit GAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAAT 5 0.125 No Hit AGATTCTACCCAGAAGACATACTCTGACACATTTCGGTTCCCGAGAAAAT 5 0.125 No Hit GAAGAAGAGAAGATGTATAAAGAGGGGAAGACAGAGAAGGAGAAGGGAGT 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 5 0.125 No Hit GGAGAAGAGCAAGGATGATGACTGATGGATTCAACAGCTGCAAAAATGTG 5 0.125 No Hit CGGGATGTGTAATGGCCCGCATTTGTCACACTAATAACTGCCCTGTTGGT 5 0.125 No Hit AGAAAAATGAGCCGGGATCCGAAGAGAAACCTGAGAGGGTGAAAGAAAAG 5 0.125 No Hit GTTACAGAGAAGAGGATATCACTTATCAAAGCATGGGAAGAGAGTGAGAA 5 0.125 No Hit AACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAA 5 0.125 No Hit ATTGATTAATTCATTTATTTCCCATCTCTCTCTTTCTCTCAGAAAATTTA 5 0.125 No Hit CAAAGAGATGGGAGGAAGAAGAATAGAACTTTCAGAAGTTGCTACTAACA 5 0.125 No Hit CTTGAAAGTTGACTTCCGTTTTTTACATGCAATGTTTATACCAGCCGTTG 5 0.125 No Hit CTTGCTTATTGTTTGGCCTTTTACTTTCACCATCGTCTTTGCCCATAAAT 5 0.125 No Hit CTTCTCCCTACTTCCTTCTCTCTTCCTTGTCAAATCTCACTCTTCCAGAT 5 0.125 No Hit CAAACGACTTTTTATATATGGTGGATGTGGGAAATCTTCAGATAATTATC 5 0.125 No Hit GTTAGATTGTAGTAAAGGGGAAGATGAAGGACAGTTTTGTTTCGCGGACA 5 0.125 No Hit GTTTGTGCAAGCTCTTTGACAGGGTACCAAAGAAGAATGTTATCACTCAA 5 0.125 No Hit GGTCGAGCAAAATCTGGCTGACCAAAGTTCTGCTGATTATAAATCCAGGT 5 0.125 No Hit CTGGAAAGGGAGCTCTAGCACAGCTCAACATCGAGACAGGGATTCCAGTC 5 0.125 No Hit CATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGA 5 0.125 No Hit TGGGTTTTGGGCCAATTCCTAACATACTATGTGCGGAGATCTTCCCTACT 5 0.125 No Hit GGAAAGGGTCAGCAGAGCAGATCAGAGTGAAGAGTGTGAGAGTGAGGGAG 5 0.125 No Hit AAGAATGTTTAGATCAGGCGTTGTTGGCTTTACATTACACGGCTCCCTTT 5 0.125 No Hit GTACATCTGGTACTATCGTCTATGCTAATCTAATTTGATCAGTTCGAATA 5 0.125 No Hit ATCTGCTTTCTCCCTGGATCAACTGATTTTTGTTAAAGAAGAAGAGTAAT 5 0.125 No Hit GGAATCAAGATGGCGCGGAAAGGCTTGATGGAACAAGACTTGAGTAAATT 5 0.125 No Hit GCTAGCAAGGACAAAGACATCGATGGTTGTTTGATGCCAAACCTCATCTA 5 0.125 No Hit CTCAATGTTCGGATTCTTTGTCCAGGCCATTGTGACCGGAAAGGGACCAC 5 0.125 No Hit ACCTTTTGAAGCTTAACAAATCTTGGGCACCAAGTATCTGTTAATTCTAA 5 0.125 No Hit TGATTCTCCTGCTGCTGTTTCTGCCAAAAAGAACAAGAAACCCAACTATT 5 0.125 No Hit TGAAAAAAGAGAGGCTGAAGGAACTGATCCGGTTGAAGTGCCAGTAAAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.1125 0.0 0.0 0.0 0.0 84-85 0.15 0.0 0.0 0.0 0.0 86-87 0.15 0.0 0.0 0.0 0.0 88-89 0.16249999999999998 0.0 0.0 0.0 0.0 90-91 0.1875 0.0 0.0 0.0 0.0 92-93 0.2875 0.0 0.0 0.0 0.0 94-95 0.4125 0.0 0.0 0.0 0.0 96-97 0.5 0.0 0.0 0.0 0.0 98-99 0.6499999999999999 0.0 0.0 0.0 0.0 100-101 0.8125 0.0 0.0 0.0 0.0 102-103 1.0 0.0 0.0 0.0 0.0 104-105 1.075 0.0 0.0 0.0 0.0 106-107 1.3 0.0 0.0 0.0 0.0 108-109 1.6124999999999998 0.0 0.0 0.0 0.0 110-111 1.7000000000000002 0.0 0.0 0.0 0.0 112-113 1.8125 0.0 0.0 0.0 0.0 114-115 2.1 0.0 0.0 0.0 0.0 116-117 2.3 0.0 0.0 0.0 0.0 118-119 2.5875 0.0 0.0 0.0 0.0 120-121 3.125 0.0 0.0 0.0 0.0 122-123 3.7 0.0 0.0 0.0 0.0 124-125 4.1 0.0 0.0 0.0 0.0 126-127 4.4125 0.0 0.0 0.0 0.0 128-129 5.0 0.0 0.0 0.0 0.0 130-131 5.775 0.0 0.0 0.0 0.0 132-133 6.2125 0.0 0.0 0.0 0.0 134-135 6.725 0.0 0.0 0.0 0.0 136-137 7.612500000000001 0.0 0.0 0.0 0.0 138-139 8.1375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTTAT 10 0.006830828 145.0 2 TTCCGCA 10 0.006830828 145.0 145 TTATCTG 10 0.006830828 145.0 5 TGTTATC 10 0.006830828 145.0 3 GCAAATC 10 0.006830828 145.0 3 TATCTGA 10 0.006830828 145.0 6 TCTGATG 10 0.006830828 145.0 8 GTTATCT 10 0.006830828 145.0 4 ATCTGAT 10 0.006830828 145.0 7 GAAATGA 20 0.00593511 29.0 40-44 >>END_MODULE Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892825 spots for SRR13695481.sra Written 892825 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra Read 892817 spots for SRR13695481.sra Written 892817 spots for SRR13695481.sra SRR ids: ['SRR13695481.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_vpv2mvbd SRR13695481.sra spots: 17856348 blocks: [[1, 892817], [892818, 1785634], [1785635, 2678451], [2678452, 3571268], [3571269, 4464085], [4464086, 5356902], [5356903, 6249719], [6249720, 7142536], [7142537, 8035353], [8035354, 8928170], [8928171, 9820987], [9820988, 10713804], [10713805, 11606621], [11606622, 12499438], [12499439, 13392255], [13392256, 14285072], [14285073, 15177889], [15177890, 16070706], [16070707, 16963523], [16963524, 17856348]] SRR13695481 file size 6046667 SRR13695481 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695481 SRR13695481_1.fastq SRR13695481_2.fastq Input file: SRR13695481_1.fastq Paired file: SRR13695481_2.fastq trimmed: SRR13695481-trimmed-pair1.fastq, SRR13695481-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 06:42:38 2025 >> started Wed Feb 12 06:42:57 2025 >> done (18.857s) 17856348 read pairs processed; of these: 108 ( 0.00%) short read pairs filtered out after trimming by size control 343 ( 0.00%) empty read pairs filtered out after trimming by size control 17855897 (100.00%) read pairs available; of these: 2035623 (11.40%) trimmed read pairs available after processing 15820274 (88.60%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 5 0.00% 20 1 0.00% 21 0 0.00% 22 3 0.00% 23 2 0.00% 24 10 0.00% 25 1 0.00% 26 4 0.00% 27 5 0.00% 28 5 0.00% 29 1 0.00% 30 3 0.00% 31 10 0.00% 32 7 0.00% 33 3 0.00% 34 5 0.00% 35 12 0.00% 36 7 0.00% 37 8 0.00% 38 8 0.00% 39 4 0.00% 40 13 0.00% 41 14 0.00% 42 13 0.00% 43 22 0.00% 44 24 0.00% 45 32 0.00% 46 35 0.00% 47 38 0.00% 48 43 0.00% 49 72 0.00% 50 103 0.00% 51 82 0.00% 52 88 0.00% 53 120 0.00% 54 97 0.00% 55 105 0.00% 56 140 0.00% 57 160 0.00% 58 170 0.00% 59 194 0.00% 60 272 0.00% 61 290 0.00% 62 366 0.00% 63 393 0.00% 64 484 0.00% 65 494 0.00% 66 591 0.00% 67 675 0.00% 68 764 0.00% 69 797 0.00% 70 1005 0.01% 71 1198 0.01% 72 1280 0.01% 73 1564 0.01% 74 1803 0.01% 75 1915 0.01% 76 2117 0.01% 77 2423 0.01% 78 2709 0.02% 79 3030 0.02% 80 3395 0.02% 81 3815 0.02% 82 4258 0.02% 83 4803 0.03% 84 5432 0.03% 85 5790 0.03% 86 6141 0.03% 87 6656 0.04% 88 7153 0.04% 89 7455 0.04% 90 8302 0.05% 91 8918 0.05% 92 9324 0.05% 93 10226 0.06% 94 11159 0.06% 95 11629 0.07% 96 12400 0.07% 97 13108 0.07% 98 13352 0.07% 99 14251 0.08% 100 15286 0.09% 101 15390 0.09% 102 16317 0.09% 103 17038 0.10% 104 18253 0.10% 105 18938 0.11% 106 19935 0.11% 107 20762 0.12% 108 21142 0.12% 109 22326 0.13% 110 23238 0.13% 111 23794 0.13% 112 24898 0.14% 113 25504 0.14% 114 25873 0.14% 115 27331 0.15% 116 28280 0.16% 117 29248 0.16% 118 29934 0.17% 119 30723 0.17% 120 32022 0.18% 121 32600 0.18% 122 33445 0.19% 123 33893 0.19% 124 35637 0.20% 125 36287 0.20% 126 36868 0.21% 127 38186 0.21% 128 38423 0.22% 129 39639 0.22% 130 40917 0.23% 131 41762 0.23% 132 41536 0.23% 133 43502 0.24% 134 43812 0.25% 135 44779 0.25% 136 45700 0.26% 137 47065 0.26% 138 47192 0.26% 139 49089 0.27% 140 49636 0.28% 141 49631 0.28% 142 50748 0.28% 143 51272 0.29% 144 52044 0.29% 145 53146 0.30% 146 53700 0.30% 147 54966 0.31% 148 56040 0.31% 149 57005 0.32% 150 57459 0.32% 151 15820274 88.60% 17855897 reads passed initial QC criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=2.12 fanout-score-rank=27 prefix-density=0.43 prefix-fanout=2.0 sequence=TTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=56.59 fanout-score-rank=1 prefix-density=0.07 prefix-fanout=4.9 sequence=AAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTG criterion=sequence-density sequence-density=0.76 sequence-density-rank=1 fanout-score=2.03 fanout-score-rank=34 prefix-density=0.75 prefix-fanout=2.0 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAA criterion=fanout-score sequence-density=0.08 sequence-density-rank=27 fanout-score=68.00 fanout-score-rank=1 prefix-density=0.46 prefix-fanout=11.5 sequence=AGAAAAGAAAACAGATTATCAAGCTTACTAGAATTATGGAAGGAATGAGTGTGGAGAACATGCACAAGATAGTGGTGGCAGTGGATGAGAGTGAGGAGAGCATGCATGCTCTTTCATGGTGTCTCAGCAACCTTATTTCTCACAACTCCACCGCCACGTTAGTCCTCCTCTAT SRR13695481 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 06:43:37 Started mapping on | Feb 12 06:43:37 Finished on | Feb 12 06:45:21 Mapping speed, Million of reads per hour | 618.09 Number of input reads | 17855897 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 16868644 Uniquely mapped reads % | 94.47% Average mapped length | 294.87 Number of splices: Total | 16715374 Number of splices: Annotated (sjdb) | 16333454 Number of splices: GT/AG | 16377023 Number of splices: GC/AG | 262521 Number of splices: AT/AC | 10535 Number of splices: Non-canonical | 65295 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.85 Insertion rate per base | 0.02% Insertion average length | 2.41 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 418128 % of reads mapped to multiple loci | 2.34% Number of reads mapped to too many loci | 45836 % of reads mapped to too many loci | 0.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.78% % of reads unmapped: other | 0.15% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 569345 569345 569345 N_multimapping 418128 418128 418128 N_noFeature 693226 16486051 934473 N_ambiguous 240380 1797 97913 UnstrandedReadsAssigned:15935038 PositiveStrandReadsAssigned:380796 NegativeStrandReadsAssigned:15836258 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695481 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695481-trimmed-pair1.fastq SRR13695481-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,855,897 reads, 15,846,424 reads pseudoaligned [quant] estimated average fragment length: 252.868 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,102 rounds 52401 SRR13695481.ke.tsv 34699 SRR13695481.se.tsv 87100 total ==> SRR13695481.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1766.13 440 15.4278 Potri.005G024800.1.v4.1 1035 783.132 234 18.5036 Potri.004G059700.1.v4.1 961 709.294 0 0 Potri.007G009000.2.v4.1 1416 1164.13 0 0 Potri.003G141000.2.v4.1 2943 2691.13 852 19.6056 Potri.016G087400.1.v4.1 270 84.893 713 520.108 Potri.015G069301.1.v4.1 564 322.971 0 0 Potri.010G195200.1.v4.1 1773 1521.13 57 2.32051 Potri.012G127500.1.v4.1 977 725.235 64 5.46483 ==> SRR13695481.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 237 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 215 Potri.001G212900.v4.1 2 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 35 SRR13695481 completed mapping pipeline successfully