Starting /dee2/code/volunteer_pipeline.sh SRR13695482 current disk space = 3049913204736 free memory = 757580836 SRR13695482 SRAfilesize 980ccdc99dee940348869727b03712a3 SRR13695482.sra SRR13695482.sra file validated SRR13695482 is paired end SRR13695482 is conventional basespace SRR13695482 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695482_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5755 37.0 37.0 37.0 37.0 37.0 2 36.32375 37.0 37.0 37.0 37.0 37.0 3 36.6025 37.0 37.0 37.0 37.0 37.0 4 36.57 37.0 37.0 37.0 37.0 37.0 5 36.583 37.0 37.0 37.0 37.0 37.0 6 36.5995 37.0 37.0 37.0 37.0 37.0 7 36.526 37.0 37.0 37.0 37.0 37.0 8 36.5385 37.0 37.0 37.0 37.0 37.0 9 36.569 37.0 37.0 37.0 37.0 37.0 10-14 36.548500000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.532 37.0 37.0 37.0 37.0 37.0 20-24 36.537400000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.50410000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.4748 37.0 37.0 37.0 37.0 37.0 35-39 36.48309999999999 37.0 37.0 37.0 37.0 37.0 40-44 36.4703 37.0 37.0 37.0 37.0 37.0 45-49 36.4208 37.0 37.0 37.0 37.0 37.0 50-54 36.396699999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.3754 37.0 37.0 37.0 37.0 37.0 60-64 36.3784 37.0 37.0 37.0 37.0 37.0 65-69 36.3309 37.0 37.0 37.0 37.0 37.0 70-74 36.330499999999994 37.0 37.0 37.0 37.0 37.0 75-79 36.2896 37.0 37.0 37.0 37.0 37.0 80-84 36.2932 37.0 37.0 37.0 37.0 37.0 85-89 36.2807 37.0 37.0 37.0 37.0 37.0 90-94 36.166999999999994 37.0 37.0 37.0 37.0 37.0 95-99 36.1847 37.0 37.0 37.0 37.0 37.0 100-104 36.152300000000004 37.0 37.0 37.0 37.0 37.0 105-109 36.1004 37.0 37.0 37.0 37.0 37.0 110-114 36.0853 37.0 37.0 37.0 37.0 37.0 115-119 36.1 37.0 37.0 37.0 37.0 37.0 120-124 36.0285 37.0 37.0 37.0 37.0 37.0 125-129 36.005 37.0 37.0 37.0 37.0 37.0 130-134 35.95360000000001 37.0 37.0 37.0 37.0 37.0 135-139 35.9545 37.0 37.0 37.0 37.0 37.0 140-144 35.8153 37.0 37.0 37.0 37.0 37.0 145-149 35.6252 37.0 37.0 37.0 37.0 37.0 150-151 35.584999999999994 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 2.0 23 2.0 24 2.0 25 1.0 26 9.0 27 7.0 28 8.0 29 14.0 30 27.0 31 34.0 32 54.0 33 68.0 34 117.0 35 310.0 36 3005.0 37 339.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.375 13.125 9.125 42.375 2 20.784116612214124 13.847700427243026 37.094747423975875 28.273435536566975 3 16.975 17.474999999999998 27.450000000000003 38.1 4 22.35 26.625 20.974999999999998 30.049999999999997 5 23.400000000000002 32.6 22.875 21.125 6 22.275 34.825 23.325000000000003 19.575 7 15.625 28.799999999999997 38.35 17.224999999999998 8 18.075 27.500000000000004 31.2 23.225 9 17.025000000000002 24.75 34.5 23.724999999999998 10-14 19.68 30.0 27.79 22.53 15-19 19.735 28.65 28.000000000000004 23.615 20-24 20.125 28.215 28.325 23.335 25-29 20.04 28.895 27.505000000000003 23.56 30-34 20.255000000000003 28.439999999999998 28.265 23.04 35-39 20.5 27.815 28.175 23.51 40-44 20.064999999999998 28.749999999999996 27.185 24.0 45-49 20.345 28.904999999999998 27.115000000000002 23.635 50-54 20.96 28.43 27.37 23.24 55-59 20.415 28.73 27.845 23.01 60-64 20.244999999999997 28.720000000000002 27.76 23.275000000000002 65-69 20.77 27.555000000000003 28.444999999999997 23.23 70-74 20.47 27.705000000000002 28.185 23.64 75-79 20.445 28.084999999999997 27.58 23.89 80-84 20.91 28.775000000000002 27.495000000000005 22.82 85-89 20.395 28.775000000000002 27.63 23.200000000000003 90-94 20.785 27.12 28.715000000000003 23.380000000000003 95-99 20.005 28.54 28.46 22.994999999999997 100-104 20.849999999999998 28.16 27.93 23.06 105-109 20.625 27.875 27.894999999999996 23.605 110-114 20.745 28.16 27.265 23.830000000000002 115-119 21.415 28.725 26.895000000000003 22.965 120-124 20.349999999999998 29.255 27.125 23.27 125-129 20.325 28.904999999999998 26.565 24.205 130-134 20.605 28.610000000000003 27.07 23.715 135-139 20.794999999999998 28.49 27.560000000000002 23.155 140-144 20.845 27.82 26.685 24.65 145-149 21.12 27.445000000000004 27.01 24.425 150-151 22.0125 26.950000000000003 27.287499999999998 23.75 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.5 19 2.5 20 2.5 21 0.5 22 1.0 23 1.5 24 1.5 25 5.0 26 7.5 27 12.5 28 17.0 29 19.5 30 16.5 31 13.5 32 25.0 33 38.0 34 49.5 35 68.0 36 98.0 37 119.5 38 121.0 39 142.0 40 166.5 41 198.0 42 244.5 43 264.5 44 267.0 45 273.0 46 292.0 47 276.5 48 234.0 49 205.5 50 189.5 51 139.0 52 99.0 53 109.5 54 80.5 55 46.5 56 46.0 57 40.5 58 26.5 59 15.0 60 9.0 61 5.0 62 3.0 63 2.5 64 1.5 65 1.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.525 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.9 #Duplication Level Percentage of deduplicated Percentage of total 1 67.8351309707242 44.025 2 19.337442218798152 25.1 3 7.588597842835131 14.774999999999999 4 3.1587057010785826 8.200000000000001 5 1.2711864406779663 4.125 6 0.46224961479198773 1.7999999999999998 7 0.11556240369799693 0.525 8 0.07704160246533129 0.4 9 0.038520801232665644 0.22499999999999998 >10 0.11556240369799693 0.8250000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACCAATTCCTTGAACCTTTGCTCCATGCATTTCTTGTAGTGCTCCTTTGT 12 0.3 No Hit CCTACTCCAATCTTAGTTCCAAGTCCAATTGTCTTGTTTTCAGTTTTAAC 11 0.27499999999999997 No Hit GTTGATAGCGAGTTAACCCGGATACAATCGGGTTACGGATTAAAGAGGAT 10 0.25 No Hit AAGCAGTCTGATGTATGGCTCTCTAACATAGGGGGCTATTCTGTTTAAGG 9 0.22499999999999998 No Hit GTTCCATAAAACTGCTTGGTTGGAGCCATGCGGCGACGTTTTCTCATTTG 8 0.2 No Hit ATTGGTGATGGGGTACTTGGGTCCAACTCCGGTGGAGTAAATCCCACTGG 8 0.2 No Hit GGATAAGCAACACAGGTGAGAACCCAGCCTTCCTCTATCTGGTCATCATC 7 0.17500000000000002 No Hit TCCACTTCCAGCTCCTTATTCATATATTTTTCCACCACGCCAGGAAGATC 7 0.17500000000000002 No Hit GCTCTAATATATCTAGAAGACACAAGGAACTTCAAGCTCTCCATTGTGCT 7 0.17500000000000002 No Hit GGGAGGGAGATTTTCTTACAGAGAATTACAAAAGAGCTTTTCACACCAAT 6 0.15 No Hit GGCCAATTCCTCCTCTGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACT 6 0.15 No Hit CTCTTGGTATAGTTGATCAACTGAACAGTGATTTCTCTTTGTTCTCTTAT 6 0.15 No Hit AAGAGGGTCATGCTGGAAAGCAAAGTTAACATACACGAGAATTAAAAATT 6 0.15 No Hit CAGGGCTTTGTTCTGCAACTTTGATGGTGTGAATTGGCCTAACCCCATCA 6 0.15 No Hit AACGAAAGTATGGAGGGATTGGGGCACCACTCTTTGGATCACGGTGCTCA 6 0.15 No Hit ATCATTGGCTTCAATTGCTTTGCTGGTCAAAATCTTGAATGTTCCGAGCC 6 0.15 No Hit CACCAACATTAATCTCACAAACCACAAGTTACAACAACAAGAGGAGCGGG 6 0.15 No Hit ACCGATACGAGCACCAATCCCGAGACGAATATTAGCTGAAAGATTGCACA 6 0.15 No Hit GTTGTCGAATCCGATTATACGGATAAAGGCGTTAGGGTAAGCTTTCTTTG 6 0.15 No Hit CTCCGGTATGGATTGTACCCTTGTGCCCTGCAAAGTCCTGATGAGTGATG 6 0.15 No Hit CACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCC 6 0.15 No Hit CGGCTGATGAGGGGTAAAACGCTGCTTGCATTTATTTGAAGATGGAGACT 5 0.125 No Hit CAGAGAAGTGCTTGGAGCTAATGCAGTGATCAGTTTAGTGAGTATACATG 5 0.125 No Hit CCCCCATCCAATGGTTCAGATTGCTGAAAAGAACCCTTTTTACCACGCTC 5 0.125 No Hit GCGTAGATTCTGTACTACAACATCTTTTCCATCATACCCTTTGCTAAAAT 5 0.125 No Hit ATTTTAACCTATGAATATCAACCAAGCAGCCTATTTACTAATGTACAAAC 5 0.125 No Hit GAAACCGGAAGATCTCGTGGTTTTGGATTTGTGACCTTCGGCAATGAGAA 5 0.125 No Hit CACGTAAACTTTGTCTCTGAATCTTCAATTTTCTCCACATTGGTGAATGA 5 0.125 No Hit GCCTTTATCATATCCATCACTGTAACCACGGTCATACCTGGCCTGCGACT 5 0.125 No Hit CGGACCCAGCACTAGCTTCAGCAAACCTGAATTCAGTTCCAAACCCTCTT 5 0.125 No Hit GTCGTCCAAGAAATAATTGTTGAGGTAGTCGTTATGACCCATATCAGACA 5 0.125 No Hit TGGGCCAACAAGGAGGCGGAATACGTCTACACCAAAAGCCACCCCACCAA 5 0.125 No Hit AGGAGGTGAAGCAAGATAGTTGGCTCTTGTCAAGGCGTGGACACGGCCCT 5 0.125 No Hit GGGCAGACTGGTACGGTTCCTGGCTTAACGTTGAGCACCTGGAAAGCCAA 5 0.125 No Hit GGAGGGTAATGTAGAGGATGTTGATGATGAGAAGGACAAGGAAGAGAAGA 5 0.125 No Hit CTCCTAAGAAATAAAATTCTTTCACCTAAGTATATTCCACGGAATAAATA 5 0.125 No Hit TTCCAAGTGCACCAAGCATGGCCCATCTGCTGTGGATCACCTCAAGCTCA 5 0.125 No Hit ATCAGCTTTTCATAGTCAACGTTGTCAAATGTGGCTCTCAAATTTCCAGG 5 0.125 No Hit CGCAACTCATGGTCTATCATAACATTGTGAGGCTTGACATCTCTATGCAT 5 0.125 No Hit AAAGAATTCTCATCGAACGGCCACCCGTTCTCATTCGTCGAAATCACCAA 5 0.125 No Hit CTCCAAGTATTTAACACGGTCTGGTCCGTACCATGGGCTCCCGGAGGGAA 5 0.125 No Hit GTCCCTCACACGTTGGTCATACATTGGATGCAAACCTTCAATGACAAGAA 5 0.125 No Hit GGTCGTAACATTATCACAAGCATTGTTTTTGAAGTGGAAGCTTCAGGCTT 5 0.125 No Hit CCGCAAAGTGACCCTTCTCTGGTGCCACACCAAGAACCAGAGTTTCGTTG 5 0.125 No Hit GTTGAAGGAATTGGGTTGTCTCAGGTTAAGAATAAATGGGCTGTTAATTT 5 0.125 No Hit CTCCGTTAGACATGCACTCCTCGAGTCCTTTCATCACTCCAGGAACATAT 5 0.125 No Hit GGTCTCTGTGTATTCTTCCATTCATTATTGGGTATATTAGTACTACTTTC 5 0.125 No Hit CTCAACAGTATTTTGCGTCTGTCAGGATCGACAGACTCCAAGCATTAGAT 5 0.125 No Hit GGGGGGAGCTGAACATCAAGTTGAAGCTGCTGTTGTTTTGGCTGGTCTCA 5 0.125 No Hit GCCATGAGAGGCAAGCCAGTGCATAGACAGATGGCCTCTTGAAGAACAAA 5 0.125 No Hit GGCAGAAGATCTTTCCAAAACCCCTGTTTCTTAAGGGGAGGCAAATTGAT 5 0.125 No Hit CCAACTCTCATTGCTATGTGCCAAGGTGATCTGTACAACCTCCAAGGCGT 5 0.125 No Hit GTAGCATTTTAAAAGATGAATCCACTTACGGTTGATTTTCTCCCGCTGAT 5 0.125 No Hit GTCACGAAATCTCCAATAACAACAGAACACGAGATAAAAACCACAAAGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.16249999999999998 0.0 0.0 0.0 0.0 84-85 0.3 0.0 0.0 0.0 0.0 86-87 0.4 0.0 0.0 0.0 0.0 88-89 0.4625 0.0 0.0 0.0 0.0 90-91 0.5625 0.0 0.0 0.0 0.0 92-93 0.6375 0.0 0.0 0.0 0.0 94-95 0.8125 0.0 0.0 0.0 0.0 96-97 1.0375 0.0 0.0 0.0 0.0 98-99 1.1625 0.0 0.0 0.0 0.0 100-101 1.2374999999999998 0.0 0.0 0.0 0.0 102-103 1.4125 0.0 0.0 0.0 0.0 104-105 1.575 0.0 0.0 0.0 0.0 106-107 1.8125 0.0 0.0 0.0 0.0 108-109 1.8875000000000002 0.0 0.0 0.0 0.0 110-111 2.1125 0.0 0.0 0.0 0.0 112-113 2.3125 0.0 0.0 0.0 0.0 114-115 2.875 0.0 0.0 0.0 0.0 116-117 3.3625 0.0 0.0 0.0 0.0 118-119 3.725 0.0 0.0 0.0 0.0 120-121 3.975 0.0 0.0 0.0 0.0 122-123 4.3125 0.0 0.0 0.0 0.0 124-125 4.6625 0.0 0.0 0.0 0.0 126-127 5.0125 0.0 0.0 0.0 0.0 128-129 5.5875 0.0 0.0 0.0 0.0 130-131 6.75 0.0 0.0 0.0 0.0 132-133 7.2375 0.0 0.0 0.0 0.0 134-135 7.862500000000001 0.0 0.0 0.0 0.0 136-137 8.524999999999999 0.0 0.0 0.0 0.0 138-139 8.787500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13695482 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695482_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.013 37.0 37.0 37.0 37.0 37.0 2 35.9225 37.0 37.0 37.0 37.0 37.0 3 36.1095 37.0 37.0 37.0 37.0 37.0 4 35.9075 37.0 37.0 37.0 37.0 37.0 5 36.158 37.0 37.0 37.0 37.0 37.0 6 36.1795 37.0 37.0 37.0 37.0 37.0 7 36.131 37.0 37.0 37.0 37.0 37.0 8 36.141 37.0 37.0 37.0 37.0 37.0 9 36.087 37.0 37.0 37.0 37.0 37.0 10-14 36.1682 37.0 37.0 37.0 37.0 37.0 15-19 36.17829999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.099450000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.02995 37.0 37.0 37.0 37.0 37.0 30-34 36.052299999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.03615 37.0 37.0 37.0 37.0 37.0 40-44 35.99655 37.0 37.0 37.0 37.0 37.0 45-49 36.022 37.0 37.0 37.0 37.0 37.0 50-54 35.90525 37.0 37.0 37.0 37.0 37.0 55-59 35.91415 37.0 37.0 37.0 37.0 37.0 60-64 35.89195 37.0 37.0 37.0 37.0 37.0 65-69 35.8433 37.0 37.0 37.0 37.0 37.0 70-74 35.80545 37.0 37.0 37.0 37.0 37.0 75-79 35.80195 37.0 37.0 37.0 37.0 37.0 80-84 35.7922 37.0 37.0 37.0 37.0 37.0 85-89 35.7106 37.0 37.0 37.0 37.0 37.0 90-94 35.67315 37.0 37.0 37.0 37.0 37.0 95-99 35.6157 37.0 37.0 37.0 37.0 37.0 100-104 35.61895 37.0 37.0 37.0 37.0 37.0 105-109 35.6211 37.0 37.0 37.0 37.0 37.0 110-114 35.55905 37.0 37.0 37.0 37.0 37.0 115-119 35.54735 37.0 37.0 37.0 37.0 37.0 120-124 35.38205 37.0 37.0 37.0 37.0 37.0 125-129 35.4538 37.0 37.0 37.0 37.0 37.0 130-134 35.19440000000001 37.0 37.0 37.0 29.8 37.0 135-139 35.27885 37.0 37.0 37.0 29.8 37.0 140-144 35.10195 37.0 37.0 37.0 27.4 37.0 145-149 35.02385 37.0 37.0 37.0 25.0 37.0 150-151 34.554125 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 2.0 15 2.0 16 2.0 17 0.0 18 0.0 19 2.0 20 1.0 21 2.0 22 2.0 23 1.0 24 2.0 25 6.0 26 17.0 27 15.0 28 8.0 29 23.0 30 39.0 31 47.0 32 82.0 33 141.0 34 260.0 35 692.0 36 2510.0 37 142.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.98496240601504 21.102756892230577 14.912280701754385 30.0 2 29.15 27.05 27.650000000000002 16.150000000000002 3 21.675 28.875 30.275000000000002 19.175 4 23.724999999999998 31.924999999999997 24.6 19.75 5 25.7 35.75 22.0 16.55 6 21.325 39.775 23.375 15.525 7 20.4 21.825 38.224999999999994 19.55 8 21.125 25.900000000000002 28.675 24.3 9 22.7 24.825 31.775 20.7 10-14 22.98 29.18 26.82 21.02 15-19 22.675 28.63 27.055 21.64 20-24 22.651132556627832 28.68143407170359 27.94139706985349 20.72603630181509 25-29 22.500625156289072 27.996999249812454 28.922230557639413 20.580145036259065 30-34 22.447244724472448 27.782778277827784 28.877887788778878 20.89208920892089 35-39 23.071153557677885 28.171408570428518 27.45137256862843 21.30606530326516 40-44 23.098464769715456 28.98934840226034 27.349102365354806 20.5630844626694 45-49 22.007200720072007 27.61776177617762 28.927892789278932 21.44714471447145 50-54 23.481174058702937 28.276413820691033 27.731386569328464 20.511025551277566 55-59 22.840710177544384 28.64216054013503 27.811952988247064 20.705176294073517 60-64 22.906145307265362 27.05635281764088 28.616430821541076 21.42107105355268 65-69 23.365 27.48 28.155 21.0 70-74 23.10077519379845 27.591897974493623 27.721930482620653 21.585396349087272 75-79 22.61339200880132 27.344101615242288 28.419262889433416 21.623243486522977 80-84 23.669999999999998 27.365000000000002 27.485 21.48 85-89 22.734546909381876 28.660732146429286 26.7003400680136 21.904380876175235 90-94 23.201160058002902 27.546377318865943 28.201410070503524 21.05105255262763 95-99 24.27 28.52 26.14 21.07 100-104 24.10602650662666 27.826956739184794 27.60190047511878 20.46511627906977 105-109 22.884576915383075 28.020604120824167 28.015603120624128 21.079215843168633 110-114 23.53617680884044 27.67138356917846 28.086404320216012 20.706035301765088 115-119 24.18362754413162 27.524128619292892 27.47912186828024 20.813121968295246 120-124 24.246061515378845 27.481870467616904 27.446861715428856 20.825206301575395 125-129 24.3498699739948 28.385677135427084 26.865373074614922 20.39907981596319 130-134 24.47734320296089 27.618285485645693 27.378213464039213 20.526157847354206 135-139 25.751437859464865 28.157039259814955 26.3615903975994 19.72993248312078 140-144 25.46627331366568 27.78638931946597 26.241312065603278 20.506025301265062 145-149 25.66641660415104 26.571642910727682 27.341835458864715 20.420105026256564 150-151 26.760035013129922 27.285231961985744 26.34738026760035 19.607352757283984 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 2.0 19 1.5 20 0.0 21 1.0 22 2.0 23 1.5 24 1.5 25 3.5 26 5.0 27 8.0 28 9.0 29 8.5 30 13.0 31 15.5 32 20.0 33 37.0 34 56.0 35 72.5 36 84.0 37 109.5 38 149.5 39 179.0 40 212.0 41 228.0 42 232.5 43 234.5 44 250.5 45 281.0 46 279.5 47 245.5 48 214.0 49 202.0 50 181.0 51 146.5 52 110.0 53 89.5 54 83.0 55 64.0 56 43.5 57 31.5 58 22.5 59 22.0 60 19.0 61 9.0 62 3.5 63 4.5 64 3.5 65 1.0 66 1.0 67 1.0 68 0.5 69 0.5 70 0.5 71 0.5 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.01 35-39 0.005 40-44 0.015 45-49 0.01 50-54 0.005 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.015 80-84 0.0 85-89 0.02 90-94 0.005 95-99 0.0 100-104 0.025 105-109 0.02 110-114 0.005 115-119 0.015 120-124 0.025 125-129 0.02 130-134 0.03 135-139 0.025 140-144 0.005 145-149 0.025 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.7 #Duplication Level Percentage of deduplicated Percentage of total 1 69.59665144596651 45.725 2 18.036529680365295 23.7 3 7.191780821917808 14.174999999999999 4 3.0060882800608826 7.9 5 1.2557077625570776 4.125 6 0.4946727549467275 1.95 7 0.076103500761035 0.35000000000000003 8 0.15220700152207 0.8 9 0.0380517503805175 0.22499999999999998 >10 0.15220700152207 1.05 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGACGTGTTAAAGCAATGGCAGCATACACGGTGAAGCTCATCACTCCTGA 11 0.27499999999999997 No Hit AGCTGCAGAGTGAGTAGAAGAGAGATGGAGTTCCCAGTGATTAGCATGGA 11 0.27499999999999997 No Hit CCAAAAGTAACAATCTGCCTGCTTTTATTTGTAATCCATGTATGATATTT 10 0.25 No Hit AATCTGAGGCACCTGGTTTAAGTTCTCGTTTTGATGGAAAGTCTAGCAGG 10 0.25 No Hit GTGGTTCAAGCCAATCAACTGGAAGAAATTGGATGCCAGGGAGATCCAGC 9 0.22499999999999998 No Hit CTGCATAGCTTATAAGCTTGTAAGAGATGGCTTCCTCCTCTATGATCTCA 8 0.2 No Hit GCAATGGCTACTCAAGCCTCTCTCTTCACCCCCACGACCCTCTCCACCCT 8 0.2 No Hit GTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGT 8 0.2 No Hit GTTCTACGAGAGCTCTGAGATGAACTTTGGTGCTGAGAATGGCTGCAAAT 8 0.2 No Hit TATTGGGGTTGATTTGAACCCCAGCAGATTCAATGAAGCAAAGAAGTTTG 7 0.17500000000000002 No Hit GTGGAAAAGGAGAGGACAAATGTTCATATCAATAAAGCAGAAGGGTACTT 7 0.17500000000000002 No Hit GGTTTCTTGGTCCTCTTGCAATTATGAGGATTTGGACTTTTTGTTTGTTC 6 0.15 No Hit AGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 6 0.15 No Hit CAAGCATTGACACAGAAGAACTGGAATAGAAACATTTTTTTCACTAATGT 6 0.15 No Hit CCCATTATATTTGCTTCTGATAGCTTTCTTGAACTAACAGAATACACTCG 6 0.15 No Hit CAGAGATTCAGGTTGGAGCACATTCCATCAGGCTGGTGGTGGTCCAGATC 6 0.15 No Hit CGCAAATCCCATTTTCTTGGCTCTATCTTTCAGGCTTAAACAAGAAAGAA 6 0.15 No Hit AAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAATGG 6 0.15 No Hit GAGGTGACAAGGAATATGATAAAGAATGAGGGTTTTGGTGCTTTATACAA 6 0.15 No Hit CAGCTGCAAGCATCTTGCTAGCAAATCAATGCCTGGCTACACTAGCTTTC 6 0.15 No Hit CCTGCTGGATTTCCTGATCATCCGCACAGAGGTTTTGAGACTGTTACATA 6 0.15 No Hit AGTCAGTACCAGAAAGGCTAATGACATTACTTCCATTGCAAGCAATGGTG 6 0.15 No Hit ATGGACCCACAATGCACACCATCTCGCTTGCAACAGTCTTGATTATGATC 6 0.15 No Hit CTGGCCGATCCTGTCCACAACAATATCTTGACCAGCCTTAAATTTCATTA 6 0.15 No Hit GCTGATTTGCCTTCTACTACTGCATTAGAGGCACCGGTTACAAGTGGGTA 5 0.125 No Hit CAGAAGATGGTCAAAAGATGATCAGCGAGCTCATGTCAATCTACGACACT 5 0.125 No Hit ACCAAGAGGTGGCAATCCTGACTCGAACACACTCATAAGCGACACTACAA 5 0.125 No Hit TTTGAATATGTGAACAGTACAGACTTCAAAGTTTTGTACCCCACTTTGAC 5 0.125 No Hit AGGTGAAAGGATCTATGACTATGATGTGTACAATGATCTTGGAAATCCTG 5 0.125 No Hit GCTTCTTCTTGGTGTCAAAGAGGTCAAATGGTGCTCTCTTAGGGACAAAG 5 0.125 No Hit ACAAGGGCTTGTTGATAGTGGATCACCAACTAGCCACTGACAAGAGAACT 5 0.125 No Hit CAGCAGTACTGGAAGCTCAAAGCAGAGCTGCTCCCTGATGAAAAGCACAG 5 0.125 No Hit AAGTGAACTACCGTGAAAGCATTTCCAAAGTAGCAGAAGTGAAGTACGTC 5 0.125 No Hit TGTCATTCAGCTTTTATATCCCCTATTCTCTTCGTGAAGATGTCTTGCTG 5 0.125 No Hit GGTGTTATTATTCGGTGTTTTAATCATTATCGTTCGATTCGCTTACGTTG 5 0.125 No Hit CTTCCAAAGCATGGAGCAGTGGAGAATGTTGGGGAGCTGTCACAGGGCTT 5 0.125 No Hit AGTAAATCCAGAAATGCATTGGTTGGAAAGGGAAAAGGCATAATTCAAGG 5 0.125 No Hit ACGATTGCTCTTTTTTTCTTTTTTGTTTCGTACCAAGGTTTGTTAGTCTT 5 0.125 No Hit CCCTGAATTACAAGACATGGGTGGCCCAACCGAAGGTGGATTCAGTGTAG 5 0.125 No Hit CTGCATCCTTACTAGTCTATAAAGAACAAAGATATGGCTCTTCCTTTAGT 5 0.125 No Hit CAAGAACGTTATGTATGAGATAGAGGGATCGTATGCACAAAGATGCTGTG 5 0.125 No Hit CTGAAGTGGTCCTGGAGGAGACGAATTCTGTTGAGATGAAGATGAGTGTC 5 0.125 No Hit TGGACATAGTGATTTTGGATACGGTGGAAGAGATGGTGGAAGGGGTAGTT 5 0.125 No Hit TCCAATTCTTACCCTCATAAACCGGCTCTTATTGATGCGGATTTGTCTAT 5 0.125 No Hit GTTGAGGATGAGGTGGCAGAGATCAAGGCAGCACATTTTGAGGAATCCAT 5 0.125 No Hit GTTTGTCAAGAACTTCAAAGGTGCAGCTCAGATACTCGAGCGGAAGGGTT 5 0.125 No Hit CAACTTCTGGCAGCAGTGTACAACTGCCGTTTTCAACCTGGGCATTTCTC 5 0.125 No Hit TATGTTGACAGGCGACTTGGAGCTGTTAGATTCTTATGATGGACACTATG 5 0.125 No Hit GGAAAATTGGGTTCTATGCCGCATATTTTTGAAGAAGAGAGGCACAAAAA 5 0.125 No Hit TCTGTAGTGTGCCACAAGCAGAACTATGCATATTCCGTCTTTTACTGCCA 5 0.125 No Hit GACTTGAGAGGAGCTGTTTGGATTCAAGCCTCACTGATGACATCTCATCC 5 0.125 No Hit CAGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 5 0.125 No Hit GCCATATTCTTTCTAGCAGCTCTTGCATTATCATACTCAAACTTCAAAAC 5 0.125 No Hit AGGAAATGTCTCCAGTTCTGTTTACATTATTGAAGAGCACCGAACCATTT 5 0.125 No Hit GTGTAAATTATGGATCGAATGGAGCTGGCATCCTTGATTTAACTGGCTAT 5 0.125 No Hit AGCATGAGTTTTGGGCATAATGATGTGAAGCCAGTTTACATCTGATGGCA 5 0.125 No Hit GACCCGCTTAATGATTGTCCTAGGAATGCCTATTACATGGATGGTGTGTT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.16249999999999998 0.0 0.0 0.0 0.0 84-85 0.3 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.5375 0.0 0.0 0.0 0.0 90-91 0.6375 0.0 0.0 0.0 0.0 92-93 0.7125 0.0 0.0 0.0 0.0 94-95 0.8625 0.0 0.0 0.0 0.0 96-97 1.0875 0.0 0.0 0.0 0.0 98-99 1.2125 0.0 0.0 0.0 0.0 100-101 1.2875 0.0 0.0 0.0 0.0 102-103 1.4875 0.0 0.0 0.0 0.0 104-105 1.675 0.0 0.0 0.0 0.0 106-107 1.9125 0.0 0.0 0.0 0.0 108-109 1.9874999999999998 0.0 0.0 0.0 0.0 110-111 2.1875 0.0 0.0 0.0 0.0 112-113 2.3875 0.0 0.0 0.0 0.0 114-115 2.95 0.0 0.0 0.0 0.0 116-117 3.4375 0.0 0.0 0.0 0.0 118-119 3.7875 0.0 0.0 0.0 0.0 120-121 4.025 0.0 0.0 0.0 0.0 122-123 4.362500000000001 0.0 0.0 0.0 0.0 124-125 4.7125 0.0 0.0 0.0 0.0 126-127 5.1375 0.0 0.0 0.0 0.0 128-129 5.7125 0.0 0.0 0.0 0.0 130-131 6.875 0.0 0.0 0.0 0.0 132-133 7.35 0.0 0.0 0.0 0.0 134-135 7.9625 0.0 0.0 0.0 0.0 136-137 8.649999999999999 0.0 0.0 0.0 0.0 138-139 8.912500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAAGTA 10 0.006830828 145.0 8 CATCAAG 10 0.006830828 145.0 6 ATCAAGT 10 0.006830828 145.0 7 >>END_MODULE Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927892 spots for SRR13695482.sra Written 927892 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra Read 927890 spots for SRR13695482.sra Written 927890 spots for SRR13695482.sra SRR ids: ['SRR13695482.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_to0324vs SRR13695482.sra spots: 18557802 blocks: [[1, 927890], [927891, 1855780], [1855781, 2783670], [2783671, 3711560], [3711561, 4639450], [4639451, 5567340], [5567341, 6495230], [6495231, 7423120], [7423121, 8351010], [8351011, 9278900], [9278901, 10206790], [10206791, 11134680], [11134681, 12062570], [12062571, 12990460], [12990461, 13918350], [13918351, 14846240], [14846241, 15774130], [15774131, 16702020], [16702021, 17629910], [17629911, 18557802]] SRR13695482 file size 6285052 SRR13695482 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695482 SRR13695482_1.fastq SRR13695482_2.fastq Input file: SRR13695482_1.fastq Paired file: SRR13695482_2.fastq trimmed: SRR13695482-trimmed-pair1.fastq, SRR13695482-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 06:07:34 2025 >> started Wed Feb 12 06:07:55 2025 >> done (20.845s) 18557802 read pairs processed; of these: 138 ( 0.00%) short read pairs filtered out after trimming by size control 2700 ( 0.01%) empty read pairs filtered out after trimming by size control 18554964 (99.98%) read pairs available; of these: 2415243 (13.02%) trimmed read pairs available after processing 16139721 (86.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 2 0.00% 20 1 0.00% 21 6 0.00% 22 4 0.00% 23 4 0.00% 24 1 0.00% 25 2 0.00% 26 4 0.00% 27 1 0.00% 28 4 0.00% 29 3 0.00% 30 5 0.00% 31 4 0.00% 32 3 0.00% 33 3 0.00% 34 6 0.00% 35 4 0.00% 36 11 0.00% 37 19 0.00% 38 15 0.00% 39 15 0.00% 40 13 0.00% 41 22 0.00% 42 18 0.00% 43 27 0.00% 44 26 0.00% 45 31 0.00% 46 30 0.00% 47 34 0.00% 48 56 0.00% 49 85 0.00% 50 83 0.00% 51 91 0.00% 52 115 0.00% 53 136 0.00% 54 113 0.00% 55 137 0.00% 56 183 0.00% 57 188 0.00% 58 234 0.00% 59 282 0.00% 60 381 0.00% 61 414 0.00% 62 433 0.00% 63 510 0.00% 64 566 0.00% 65 635 0.00% 66 665 0.00% 67 820 0.00% 68 882 0.00% 69 1132 0.01% 70 1347 0.01% 71 1434 0.01% 72 1740 0.01% 73 1981 0.01% 74 2159 0.01% 75 2322 0.01% 76 2636 0.01% 77 2906 0.02% 78 3219 0.02% 79 3627 0.02% 80 4006 0.02% 81 4729 0.03% 82 5175 0.03% 83 5753 0.03% 84 6543 0.04% 85 7131 0.04% 86 7439 0.04% 87 7948 0.04% 88 8566 0.05% 89 9161 0.05% 90 9974 0.05% 91 10842 0.06% 92 11661 0.06% 93 12991 0.07% 94 13225 0.07% 95 14382 0.08% 96 15349 0.08% 97 16146 0.09% 98 16688 0.09% 99 17412 0.09% 100 18568 0.10% 101 18974 0.10% 102 20246 0.11% 103 20763 0.11% 104 21654 0.12% 105 23381 0.13% 106 24308 0.13% 107 25274 0.14% 108 25871 0.14% 109 26863 0.14% 110 27138 0.15% 111 28556 0.15% 112 29568 0.16% 113 30693 0.17% 114 32149 0.17% 115 32671 0.18% 116 34003 0.18% 117 35479 0.19% 118 36505 0.20% 119 37429 0.20% 120 38357 0.21% 121 38853 0.21% 122 40167 0.22% 123 41235 0.22% 124 42721 0.23% 125 43118 0.23% 126 44446 0.24% 127 45228 0.24% 128 46987 0.25% 129 47399 0.26% 130 48436 0.26% 131 48868 0.26% 132 48741 0.26% 133 50245 0.27% 134 50946 0.27% 135 51890 0.28% 136 52920 0.29% 137 54771 0.30% 138 55251 0.30% 139 57489 0.31% 140 56591 0.30% 141 58774 0.32% 142 59560 0.32% 143 59008 0.32% 144 61257 0.33% 145 60767 0.33% 146 62947 0.34% 147 63536 0.34% 148 65106 0.35% 149 65724 0.35% 150 66861 0.36% 151 16139721 86.98% 18554964 reads passed initial QC criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=25 prefix-density=0.31 prefix-fanout=2.0 sequence=GTGTTGTCGAATCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=28 fanout-score=50.13 fanout-score-rank=1 prefix-density=0.07 prefix-fanout=5.5 sequence=AAACAGAATATTTACTTTTAGCGCAAGTTTCAATTCATGAGCTCGTTACATCACAAGTTAACATTACAAGCCAATAGTCCCAGCACAGAAAAAACTCTTTTGTTGCTCACTTTCCGGGAACGAAGTTTGTGGCATATGCCCAGGCGTTGTTGTTTACTGGGTCAGAAAGGTGGTCAGCCAGGTTCTCCAGTGGTCCCTTTCCGGTCACAATGGCCTGGACAAAGAATCCGAACATTGAGAACATAGCCAACCT criterion=sequence-density sequence-density=1.13 sequence-density-rank=1 fanout-score=2.26 fanout-score-rank=22 prefix-density=1.16 prefix-fanout=2.2 sequence=CCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=23.37 fanout-score-rank=1 prefix-density=0.05 prefix-fanout=2.6 sequence=AATGGCCATGTAAAACACAATATTTTTCAGTATGAAAGCTTTAGTGATAGCTATTCTTATAGCTACCATTGCCTTCTCTCCCTTATCCATGGCAGCTCGAGAATTGGTCGACTATGGAAAAGATAGCGTTACCGTCAATATCCCATCAACTGGCGATGTATCATCTAGAAGCCAGCCTCCTACCTATGCCCCACGAACTGGCAGTGGATGTAGCATTTACCAACGAGATTGTCCTAAGAAAAAACCTTGTAATCCTTACAAGCGTAGCTGCCATCGCCCTTGAAAATGAAAGAGTAGTTTGATTTGGGTCCATTATCTAGTGGTAAAAGCTGTGAGCTCAAAGCACCAGGGCTATCTATTACTTTCATTTCCATTACCAATGTAATTATATGGTCGTTGGAAATTAAATAAAAGCTCCGAGTGAGCCATGGCAGATATGCATATGCTACAGGTTTCCTTTAGTACTATTGCAATCCTGTAAATGTTACCTATGAACGTTTTGTAGTCTTTT SRR13695482 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 06:09:34 Started mapping on | Feb 12 06:09:34 Finished on | Feb 12 06:11:52 Mapping speed, Million of reads per hour | 484.04 Number of input reads | 18554964 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 17181525 Uniquely mapped reads % | 92.60% Average mapped length | 293.95 Number of splices: Total | 16699875 Number of splices: Annotated (sjdb) | 16298710 Number of splices: GT/AG | 16357768 Number of splices: GC/AG | 251640 Number of splices: AT/AC | 9862 Number of splices: Non-canonical | 80605 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.85 Insertion rate per base | 0.02% Insertion average length | 2.36 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 521689 % of reads mapped to multiple loci | 2.81% Number of reads mapped to too many loci | 84579 % of reads mapped to too many loci | 0.46% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.97% % of reads unmapped: other | 0.16% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 851970 851970 851970 N_multimapping 521689 521689 521689 N_noFeature 749287 16683045 1021678 N_ambiguous 329399 1823 102042 UnstrandedReadsAssigned:16102839 PositiveStrandReadsAssigned:496657 NegativeStrandReadsAssigned:16057805 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695482 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695482-trimmed-pair1.fastq SRR13695482-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,554,964 reads, 16,219,119 reads pseudoaligned [quant] estimated average fragment length: 239.994 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,060 rounds 52401 SRR13695482.ke.tsv 34699 SRR13695482.se.tsv 87100 total ==> SRR13695482.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1779.01 722 20.1234 Potri.005G024800.1.v4.1 1035 796.006 503 31.3324 Potri.004G059700.1.v4.1 961 722.069 4 0.274678 Potri.007G009000.2.v4.1 1416 1177.01 0 0 Potri.003G141000.2.v4.1 2943 2704.01 1220.07 22.3727 Potri.016G087400.1.v4.1 270 87.8106 932 526.272 Potri.015G069301.1.v4.1 564 332.272 0 0 Potri.010G195200.1.v4.1 1773 1534.01 150 4.84848 Potri.012G127500.1.v4.1 977 738.044 187 12.5632 ==> SRR13695482.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 367 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 241 Potri.001G212900.v4.1 498 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 6 Potri.001G416900.v4.1 1 Potri.001G452600.v4.1 0 SRR13695482 completed mapping pipeline successfully