Starting /dee2/code/volunteer_pipeline.sh SRR13695483 current disk space = 3050326962176 free memory = 1578640820 SRR13695483 SRAfilesize 57466b331d0e086260dfbf972d5cdee0 SRR13695483.sra SRR13695483.sra file validated SRR13695483 is paired end SRR13695483 is conventional basespace SRR13695483 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695483_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6295 37.0 37.0 37.0 37.0 37.0 2 36.24725 37.0 37.0 37.0 37.0 37.0 3 36.6315 37.0 37.0 37.0 37.0 37.0 4 36.606 37.0 37.0 37.0 37.0 37.0 5 36.5925 37.0 37.0 37.0 37.0 37.0 6 36.5245 37.0 37.0 37.0 37.0 37.0 7 36.536 37.0 37.0 37.0 37.0 37.0 8 36.5645 37.0 37.0 37.0 37.0 37.0 9 36.5195 37.0 37.0 37.0 37.0 37.0 10-14 36.5825 37.0 37.0 37.0 37.0 37.0 15-19 36.53 37.0 37.0 37.0 37.0 37.0 20-24 36.5036 37.0 37.0 37.0 37.0 37.0 25-29 36.4769 37.0 37.0 37.0 37.0 37.0 30-34 36.426300000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.5021 37.0 37.0 37.0 37.0 37.0 40-44 36.4251 37.0 37.0 37.0 37.0 37.0 45-49 36.420899999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.436400000000006 37.0 37.0 37.0 37.0 37.0 55-59 36.3953 37.0 37.0 37.0 37.0 37.0 60-64 36.4041 37.0 37.0 37.0 37.0 37.0 65-69 36.3686 37.0 37.0 37.0 37.0 37.0 70-74 36.338499999999996 37.0 37.0 37.0 37.0 37.0 75-79 36.305499999999995 37.0 37.0 37.0 37.0 37.0 80-84 36.239 37.0 37.0 37.0 37.0 37.0 85-89 36.28589999999999 37.0 37.0 37.0 37.0 37.0 90-94 36.220200000000006 37.0 37.0 37.0 37.0 37.0 95-99 36.1716 37.0 37.0 37.0 37.0 37.0 100-104 36.1601 37.0 37.0 37.0 37.0 37.0 105-109 36.1483 37.0 37.0 37.0 37.0 37.0 110-114 36.0994 37.0 37.0 37.0 37.0 37.0 115-119 36.0674 37.0 37.0 37.0 37.0 37.0 120-124 36.0788 37.0 37.0 37.0 37.0 37.0 125-129 35.9928 37.0 37.0 37.0 37.0 37.0 130-134 36.0848 37.0 37.0 37.0 37.0 37.0 135-139 35.978 37.0 37.0 37.0 37.0 37.0 140-144 35.946200000000005 37.0 37.0 37.0 37.0 37.0 145-149 35.746300000000005 37.0 37.0 37.0 37.0 37.0 150-151 35.54225 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 2.0 26 4.0 27 10.0 28 13.0 29 18.0 30 24.0 31 28.0 32 46.0 33 61.0 34 129.0 35 343.0 36 2993.0 37 329.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.199999999999996 11.525 9.049999999999999 44.224999999999994 2 20.296407937704096 14.142175332830947 35.97086159256469 29.590555136900278 3 19.05 16.975 27.150000000000002 36.825 4 22.575 23.775 24.3 29.349999999999998 5 25.1 30.325000000000003 24.425 20.150000000000002 6 20.45 36.35 24.2 19.0 7 17.150000000000002 27.275 38.35 17.224999999999998 8 19.625 27.150000000000002 30.85 22.375 9 17.724999999999998 22.725 34.875 24.675 10-14 19.78 29.770000000000003 27.800000000000004 22.650000000000002 15-19 19.61 27.905 28.165000000000003 24.32 20-24 20.105 29.145 26.845000000000002 23.905 25-29 20.19 28.945 27.3 23.565 30-34 19.84 29.015 27.12 24.025 35-39 20.794999999999998 28.765 27.134999999999998 23.305 40-44 20.04 28.16 28.055000000000003 23.745 45-49 20.835 28.455000000000002 27.815 22.895 50-54 20.775 28.74 26.875 23.61 55-59 20.915 28.615000000000002 27.13 23.34 60-64 20.195 29.145 27.21 23.45 65-69 19.814999999999998 28.549999999999997 27.67 23.965 70-74 20.055 28.439999999999998 27.735 23.77 75-79 20.035 28.634999999999998 27.735 23.595 80-84 20.435 28.29 27.315 23.96 85-89 20.47 27.939999999999998 28.255000000000003 23.335 90-94 20.75 27.985 27.345000000000002 23.919999999999998 95-99 20.805 28.549999999999997 27.065 23.580000000000002 100-104 21.34 27.794999999999998 27.52 23.345 105-109 20.64 28.749999999999996 27.455000000000002 23.155 110-114 21.81 28.18 27.005000000000003 23.005 115-119 20.75 27.944999999999997 27.32 23.985 120-124 20.82 28.68 27.034999999999997 23.465 125-129 20.48 28.09 28.08 23.35 130-134 21.310000000000002 28.599999999999998 26.840000000000003 23.25 135-139 21.099999999999998 28.465 27.1 23.335 140-144 21.154999999999998 28.754999999999995 26.69 23.400000000000002 145-149 19.755 28.38 27.16 24.705 150-151 20.525 28.8375 26.450000000000003 24.1875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.0 20 1.0 21 1.0 22 1.0 23 1.5 24 1.0 25 3.5 26 4.5 27 6.5 28 10.5 29 11.0 30 14.5 31 20.0 32 28.5 33 33.0 34 42.0 35 64.5 36 78.5 37 108.0 38 152.5 39 170.0 40 189.0 41 213.5 42 229.5 43 235.5 44 250.0 45 288.5 46 276.5 47 234.0 48 227.5 49 224.5 50 191.5 51 148.0 52 117.5 53 103.5 54 81.0 55 53.5 56 49.0 57 42.0 58 23.0 59 15.0 60 20.0 61 12.5 62 5.0 63 5.5 64 3.5 65 3.0 66 1.5 67 1.0 68 0.5 69 0.0 70 0.5 71 0.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.475 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.1 #Duplication Level Percentage of deduplicated Percentage of total 1 71.59913169319826 49.475 2 18.849493487698986 26.05 3 5.824891461649783 12.075 4 2.279305354558611 6.3 5 0.5788712011577424 2.0 6 0.5065123010130246 2.1 7 0.1447178002894356 0.7000000000000001 8 0.1447178002894356 0.8 9 0.0361794500723589 0.22499999999999998 >10 0.0361794500723589 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTTATTGCAAACATATTCCAGCTAATAAGCTGCATTCTTGGAAACTATC 11 0.27499999999999997 No Hit CACCAAACATTTTCAATAAAAGCAGCACAAACAAAAAAAAATCCTTCAAG 9 0.22499999999999998 No Hit GTCCTTCTCTGATTTCAATTCTTCAAACTCTTCCAATGAGACCTTGCACG 8 0.2 No Hit CTCGAATACATAATTGCAACTACAATTATGGTTGTAAAAAGGACCTTTCG 8 0.2 No Hit GTCCATGATGGCTTATTGGGTTGCCACGTAGGCTTAGATTGTTGTGTAGA 8 0.2 No Hit CCGGCCTGAAAATCTGCCCATACGGACCAGTACGAACACTGTCCATGGTA 8 0.2 No Hit GCCATGACATTGTAGGTGCATGAAATCCATAGTCCATAAGACGCTTGGCA 7 0.17500000000000002 No Hit GCACAAGAAAGACCAGCAGAGCCAGCACCAACAACGACAACATCAGTGTC 7 0.17500000000000002 No Hit CATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTC 7 0.17500000000000002 No Hit GCACCAGAGTAGTTCCACAAAGAGCATCATAGGACCTTGTCGAGGCCATG 7 0.17500000000000002 No Hit GCGAGGCCATGTCATAACAGGACTAGCTTCTTGGTTGGAAGAGCTTCTCT 6 0.15 No Hit CAATAAATTTTGCTCCTTCCTCAAATTATCTTCCAAATCTTCCAACTTCT 6 0.15 No Hit CTTTGGAATTTGAAGGAACTCAGAGCTCAGCCCGTGTTGATCCAAAACTC 6 0.15 No Hit CTCCTGTTAAAAACACTTCTTTCACATCCTCAGGGATTTCATTGAGAATA 6 0.15 No Hit TCAATTCATGAGCTCGTTACATCACAAGTTAACATTACAAGCCAATAGTC 6 0.15 No Hit CCTGACCAGAACTGAGATTTGCTCAGCACCAGCACCCCTCAGTGCACCCC 6 0.15 No Hit CCCAAGTACTGTTTAGCACGACACTCAAGGCTATCAAATTCATTAAAACA 6 0.15 No Hit GGGTAATAGGCTATTACTTCTTGAAGTATCATGTCTACAGCAACAATGAA 6 0.15 No Hit GGTAAATCCAACTATTCCCATAAATAAGTGGTTTTTTGTTACTCCTTTAA 6 0.15 No Hit AGCCGACATTGCATTTGCAATATCAGGCATGATAAACTGGTACAAAATGA 6 0.15 No Hit GCTGCCATTGCTTCGAAAACCAAAAGAAGCGGACTTCCGGAGCTTATTGA 6 0.15 No Hit GGGGTATGGTAGGCACACCAGCAATCCTAAGACCAAAACTAAGAGTCCAA 6 0.15 No Hit CCTCAATTCAAGCTAAATTTCAACTACACAGGCAGGCATGCCCATTCTAA 6 0.15 No Hit CTTCGGGAGCAAGCTCTTCCCTGCCAACATAAGGCATGAGAAGCATTGCT 6 0.15 No Hit CCCAGGCCTTCCTCCATCATAGCCACATTGATGTATGTTGCAGCAGTGTT 5 0.125 No Hit CCTTACAGTACATAAAAGTCAGGTATTTCTCGACTGAAAGAGACAACGAG 5 0.125 No Hit CCTCAGTGCATCCAAACATGGGTAGCTTCCACATAGTCCAGTAGCGTCCA 5 0.125 No Hit TCCAGTTATAGAAGTCAGGATTAGCTGCTTGCTTGCTGCCCAGTATGCCA 5 0.125 No Hit CTCTGCTTCTCTTTCTTCTAGCAGTTCACCAATACAAGCAATTACCTCAA 5 0.125 No Hit TCTAGCTGTAGGAGAGCTCCCATTTTGATGATATCAAATCGAAAAATAGT 5 0.125 No Hit GGCCCTTGGTGGATAGGCCTAAATGTTTGCAGGACAAGAAGTTGCTGTCA 5 0.125 No Hit CTCAAAAGTAGTTCCAAGTCTGAAGAACTTCTCATCTATTTGTTGATCCC 5 0.125 No Hit CACAGCTGCCATACATTTGTGCATTGCAAACTTGTTGTCGTTCTAAAGTC 5 0.125 No Hit CTGCTGTATATCGAGTGGCCATGTTCTTTGCACTCAATCTTGCTCTCTCA 5 0.125 No Hit TTCGTATCACCAGAACAACCAAACTTCAAGGACCTCCCAGAGTAAAACTA 5 0.125 No Hit CGGTTGCTGTGGCTGTAGTGGTGATGGAGTGATGGCTTTCTAGTGGTGCC 5 0.125 No Hit ATCTCTTGGACCAATACGACCATCATCAATAGCCTCTGCAAGCCCTTCTT 5 0.125 No Hit GCCAGACCAATTGACAGGGACACTCAGCGAAGATGAAGCACCCGTTGCCA 5 0.125 No Hit ATAAGAAGGGCAGAGCAGCACTACTAATCCAACATAAAGAATTGATAAAG 5 0.125 No Hit GGAAGATAGGATCAGTGTTAACGCACCCTGGCTTGAGAATATCAGCCCAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.0625 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.075 0.0 0.0 0.0 0.0 88-89 0.15 0.0 0.0 0.0 0.0 90-91 0.2625 0.0 0.0 0.0 0.0 92-93 0.4125 0.0 0.0 0.0 0.0 94-95 0.5375000000000001 0.0 0.0 0.0 0.0 96-97 0.6 0.0 0.0 0.0 0.0 98-99 0.6875 0.0 0.0 0.0 0.0 100-101 0.7375 0.0 0.0 0.0 0.0 102-103 0.8125 0.0 0.0 0.0 0.0 104-105 0.9624999999999999 0.0 0.0 0.0 0.0 106-107 1.0750000000000002 0.0 0.0 0.0 0.0 108-109 1.25 0.0 0.0 0.0 0.0 110-111 1.55 0.0 0.0 0.0 0.0 112-113 1.7374999999999998 0.0 0.0 0.0 0.0 114-115 2.0 0.0 0.0 0.0 0.0 116-117 2.275 0.0 0.0 0.0 0.0 118-119 2.6125 0.0 0.0 0.0 0.0 120-121 2.8625 0.0 0.0 0.0 0.0 122-123 3.2125 0.0 0.0 0.0 0.0 124-125 3.6875 0.0 0.0 0.0 0.0 126-127 4.25 0.0 0.0 0.0 0.0 128-129 4.825 0.0 0.0 0.0 0.0 130-131 5.525 0.0 0.0 0.0 0.0 132-133 6.125 0.0 0.0 0.0 0.0 134-135 6.5625 0.0 0.0 0.0 0.0 136-137 6.987500000000001 0.0 0.0 0.0 0.0 138-139 7.475 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGTTCT 10 0.006830828 145.0 9 GTCTGAA 25 8.7132835E-4 87.0 145 >>END_MODULE SRR13695483 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695483_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.20325 37.0 37.0 37.0 37.0 37.0 2 36.3195 37.0 37.0 37.0 37.0 37.0 3 36.273 37.0 37.0 37.0 37.0 37.0 4 36.377 37.0 37.0 37.0 37.0 37.0 5 36.448 37.0 37.0 37.0 37.0 37.0 6 36.2395 37.0 37.0 37.0 37.0 37.0 7 36.3585 37.0 37.0 37.0 37.0 37.0 8 36.3265 37.0 37.0 37.0 37.0 37.0 9 36.361 37.0 37.0 37.0 37.0 37.0 10-14 36.3564 37.0 37.0 37.0 37.0 37.0 15-19 36.2729 37.0 37.0 37.0 37.0 37.0 20-24 36.26905 37.0 37.0 37.0 37.0 37.0 25-29 36.238550000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.15815 37.0 37.0 37.0 37.0 37.0 35-39 36.17645 37.0 37.0 37.0 37.0 37.0 40-44 36.11995 37.0 37.0 37.0 37.0 37.0 45-49 36.158849999999994 37.0 37.0 37.0 37.0 37.0 50-54 36.09595 37.0 37.0 37.0 37.0 37.0 55-59 36.03625 37.0 37.0 37.0 37.0 37.0 60-64 36.03405 37.0 37.0 37.0 37.0 37.0 65-69 35.99185 37.0 37.0 37.0 37.0 37.0 70-74 36.013250000000006 37.0 37.0 37.0 37.0 37.0 75-79 35.97595 37.0 37.0 37.0 37.0 37.0 80-84 35.984249999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.87415 37.0 37.0 37.0 37.0 37.0 90-94 35.84885 37.0 37.0 37.0 37.0 37.0 95-99 35.869150000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.862049999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.85715 37.0 37.0 37.0 37.0 37.0 110-114 35.756150000000005 37.0 37.0 37.0 37.0 37.0 115-119 35.71125 37.0 37.0 37.0 37.0 37.0 120-124 35.74005 37.0 37.0 37.0 37.0 37.0 125-129 35.694950000000006 37.0 37.0 37.0 37.0 37.0 130-134 35.56609999999999 37.0 37.0 37.0 37.0 37.0 135-139 35.561699999999995 37.0 37.0 37.0 37.0 37.0 140-144 35.489850000000004 37.0 37.0 37.0 37.0 37.0 145-149 35.3975 37.0 37.0 37.0 37.0 37.0 150-151 35.080375 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 4.0 14 0.0 15 0.0 16 1.0 17 0.0 18 2.0 19 1.0 20 1.0 21 2.0 22 5.0 23 2.0 24 3.0 25 6.0 26 7.0 27 9.0 28 10.0 29 20.0 30 23.0 31 38.0 32 62.0 33 89.0 34 221.0 35 536.0 36 2710.0 37 247.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.94181088537748 21.11863556558816 13.243039879608729 29.696513669425634 2 27.224999999999998 25.224999999999998 31.724999999999998 15.825 3 20.1 26.150000000000002 33.275 20.474999999999998 4 24.05 32.300000000000004 23.75 19.900000000000002 5 24.474999999999998 36.0 23.175 16.35 6 20.200000000000003 39.324999999999996 24.175 16.3 7 19.275000000000002 22.0 39.675 19.05 8 21.25 24.125 31.25 23.375 9 21.05 23.0 31.724999999999998 24.224999999999998 10-14 23.125 28.595 26.919999999999998 21.36 15-19 22.66 28.13 28.88 20.330000000000002 20-24 23.10577644411103 28.172043010752688 28.012003000750184 20.710177544386095 25-29 22.210552638159538 28.172043010752688 28.52213053263316 21.09527381845461 30-34 21.145286321580397 28.727181795448864 28.497124281070267 21.630407601900476 35-39 22.05051262815704 28.502125531382845 27.581895473868467 21.865466366591647 40-44 22.570642660665165 28.402100525131285 28.00200050012503 21.02525631407852 45-49 22.500625156289072 28.322080520130033 28.767191797949486 20.41010252563141 50-54 23.15078769692423 27.826956739184794 27.9869967491873 21.035258814703674 55-59 22.135533883470867 28.527131782945737 27.831957989497376 21.50537634408602 60-64 22.32058014503626 27.286821705426355 29.052263065766443 21.340335083770942 65-69 22.595648912228057 26.616654163540886 28.537134283570893 22.250562640660164 70-74 22.715678919729932 27.58689672418104 28.422105526381596 21.275318829707427 75-79 22.34558639659915 28.212053013253314 27.851962990747687 21.59039759939985 80-84 22.060515128782196 28.272068017004255 28.157039259814955 21.510377594398598 85-89 22.415603900975245 27.41185296324081 29.29732433108277 20.875218804701177 90-94 23.765941485371343 27.516879219804952 27.62690672668167 21.090272568142034 95-99 23.335833958489623 28.392098024506122 27.49187296824206 20.78019504876219 100-104 23.115778944736185 27.711927981995498 27.426856714178545 21.745436359089773 105-109 22.210552638159538 28.132033008252062 27.97199299824956 21.685421355338836 110-114 23.135783945986496 28.24206051512878 27.696924231057764 20.92523130782696 115-119 23.475868967241812 28.742185546386594 27.66191547886972 20.120030007501878 120-124 24.026006501625407 27.911977994498628 27.306826706676667 20.7551887971993 125-129 24.281070267566893 28.057014253563388 26.991747936984247 20.67016754188547 130-134 24.7823911955978 28.649324662331168 26.97848924462231 19.589794897448726 135-139 24.79743923176953 28.333500050015004 27.13313994198259 19.73592077623287 140-144 24.85621405351338 27.81695423855964 26.776694173543387 20.550137534383595 145-149 25.882764829448835 27.748324497349202 26.90807242172652 19.460838251475444 150-151 25.372200675591145 28.337295133241586 25.88514950581759 20.405354685349682 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 1.5 13 1.0 14 0.5 15 0.5 16 0.0 17 0.0 18 2.5 19 3.0 20 0.5 21 0.0 22 1.0 23 1.5 24 4.5 25 7.5 26 6.0 27 8.0 28 12.5 29 13.0 30 16.5 31 18.5 32 28.5 33 44.5 34 55.0 35 87.0 36 98.0 37 117.5 38 149.0 39 173.5 40 190.0 41 204.5 42 240.0 43 257.0 44 276.5 45 285.5 46 270.5 47 227.5 48 205.0 49 207.0 50 177.0 51 130.5 52 100.5 53 89.5 54 72.0 55 47.0 56 41.5 57 37.0 58 20.0 59 18.5 60 16.5 61 10.0 62 5.5 63 1.5 64 3.0 65 3.0 66 1.5 67 1.5 68 0.5 69 0.0 70 0.0 71 1.0 72 1.5 73 0.5 74 0.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.325 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.025 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.05 135-139 0.03 140-144 0.025 145-149 0.03 150-151 0.08750000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.39999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 72.0821325648415 50.025 2 18.443804034582133 25.6 3 5.79971181556196 12.075 4 2.161383285302594 6.0 5 0.6844380403458213 2.375 6 0.5043227665706052 2.1 7 0.10806916426512969 0.525 8 0.1440922190201729 0.8 9 0.03602305475504323 0.22499999999999998 >10 0.03602305475504323 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTTGCTTCTCAATCCACTTGATTGTAAAAGAATCATGTATTTGATGTG 11 0.27499999999999997 No Hit AATAATTCTTGCTTCGTCAAAGGCATAGACTCCAACTAGTTAGGGATTGG 9 0.22499999999999998 No Hit GTCCTTCCCTGATTGCATAGATAAGATTGCTTTACCCGAGAACAAGGTTG 8 0.2 No Hit CAGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 8 0.2 No Hit CTCCCGTTCAAGTTTTCTCCATTCTCTAAAAATCTCCAGAAGCGACAATG 8 0.2 No Hit CTGTGATTTTGTTGATTGATTAAGGAGCGATGGAAGGAGATAGGGGGTGG 8 0.2 No Hit CGGAACAGTTGCCCATGAATTCATTGTGGACTTGAGAGGCGTTAAGAACA 7 0.17500000000000002 No Hit GATCCTTGGAGTCTCCATCTTCAAAACTGCATACATAGGTCACTGAGTCC 7 0.17500000000000002 No Hit GTTGAGAGTTTTTTCACGGAGTATCATCCTTCTACTTATCTTTCACATTC 7 0.17500000000000002 No Hit TGTGTTTACTGGATTGCTTGAGCATTTTAGAACTGATGCAGAGATAGCTA 6 0.15 No Hit GATAAATGTTGGCTTGCAATCTCAGACAACCATTTCAATGTGGCTAGTAT 6 0.15 No Hit CGTGTTAGAGAAATTAGTGCTGTAGTTCCTGAGTTCCATGCTCGATTTTC 6 0.15 No Hit GTCAAGATCATTAGCCTTGCTGCATTGAACAAGAATGAAGCTCTGAATGG 6 0.15 No Hit TCTTGATCCTGAACAGAATAACACATTCAACGATCACTATCTGAATGTTC 6 0.15 No Hit CAGTGTTTTGTGCAGTGTTTTTTGATTTCATATTATCTGGATTGAATATA 6 0.15 No Hit CCTGGCTGACCACCTTTCTGACCCAGTAAACAACAACGCCTGGGCATATG 6 0.15 No Hit CTCCTGATGGAAAATTAGATTGGGGTATCCAGGGAGAAGAGCTCAATAAG 6 0.15 No Hit CCTGCACAGGCCAACATGGTTGCACCATTCAACGGCCTCAAGTCTACCTC 6 0.15 No Hit GGAGTAACGATGGAGTGTGTGGTACAGGGAATTATAGAGACACAGCATGT 6 0.15 No Hit TATTCTTCACTTGAATCAAGATGACAGGAAACATGCTACAATTGCGAAGA 6 0.15 No Hit ATCTGCATCTGGTTCATATACTGCAGGTCGTCCCTTAGAGGATGCTGATT 6 0.15 No Hit GTTTATTTTGCTAGTGGTTGTGACTTAAATCTCACTGTTGAGATGCTCAC 6 0.15 No Hit TGTAGCTTTCTCCAATTTCTTTTACACAGTCAAAAACCCTTTAGCAAAAC 6 0.15 No Hit CTTATGGTCAAGTTCTTCAAGGCAGGAACCAAGCAAGACTACTACTTCTG 5 0.125 No Hit AATGAAAGCTATGGCACCATAAAGTTTGTTGACATAAGCTCAGATGAGTA 5 0.125 No Hit CTTCCTCTATGCTTGCACGCATGCAGGAGCTCAATCTGTGACTTTCGACT 5 0.125 No Hit CTTCAATCGTAAATCACAAATACATACACGTTTACTCATCAGCTCGAAAA 5 0.125 No Hit TTTTTTTTTTTTTCGTAATTTGATCGAATATGAGCAACTTCAATTTCGAT 5 0.125 No Hit AGAGAATACAATTATAGGGATGATGACCGGTATGGTAAATACGGGGATTC 5 0.125 No Hit AATTCTTTCTTCTAAATCACTTTGTTAATTTCTGATACCAAGCAGTGTAT 5 0.125 No Hit ACCACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCC 5 0.125 No Hit GCTGCTGGTATTTTCATCCCAGAATTCCTAACAAAGATTGGCATCCTAAA 5 0.125 No Hit GTTTGCTTCATGATGAGAAATATTTTGGCTCTAGTTTAAGCAATGCGCTG 5 0.125 No Hit CAGGATATTGCTCTTTCTGAACTGGCTCCTACCCACCCAATAAGGCTGGG 5 0.125 No Hit AGTTTAAAGAGTTTGACAAGGAACCGGCTGGATATCAACAATTAGATGAA 5 0.125 No Hit TGCTATTCTCTACCAGAGACAGCTGCCTCTGTGGCAATTGCTGCTACAGC 5 0.125 No Hit GCCACAATTTAAGCACTTGGCTGATAAGATTGTTCTAACTGTGGCAGAAG 5 0.125 No Hit AGATGAGGCCTAACGTAAGTATTGAATTCCTCTGGTGGCTCTCTTTAACT 5 0.125 No Hit GCAGCATTCTGTTTATCTTTTCTTCATCTGTTCTCTAATAAAGTAAGACG 5 0.125 No Hit CGTGCTATGAAGTTCTCTGTCTCGCCTGTTGTGCGTGTGGCCGTTCAGTG 5 0.125 No Hit GTCTGATGTTGATGTTGTTGTAGCACCTCCCTTTGTTTACATCGATCAGG 5 0.125 No Hit CTCACTACTTAACGATGTGGCTTTTTTCTGGGTAGTTCTAATATTCTTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.0625 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.075 0.0 0.0 0.0 0.0 88-89 0.15 0.0 0.0 0.0 0.0 90-91 0.2625 0.0 0.0 0.0 0.0 92-93 0.4125 0.0 0.0 0.0 0.0 94-95 0.5375000000000001 0.0 0.0 0.0 0.0 96-97 0.6 0.0 0.0 0.0 0.0 98-99 0.6875 0.0 0.0 0.0 0.0 100-101 0.7375 0.0 0.0 0.0 0.0 102-103 0.8125 0.0 0.0 0.0 0.0 104-105 0.9624999999999999 0.0 0.0 0.0 0.0 106-107 1.0750000000000002 0.0 0.0 0.0 0.0 108-109 1.25 0.0 0.0 0.0 0.0 110-111 1.55 0.0 0.0 0.0 0.0 112-113 1.75 0.0 0.0 0.0 0.0 114-115 2.025 0.0 0.0 0.0 0.0 116-117 2.3 0.0 0.0 0.0 0.0 118-119 2.6375 0.0 0.0 0.0 0.0 120-121 2.8875 0.0 0.0 0.0 0.0 122-123 3.2375 0.0 0.0 0.0 0.0 124-125 3.7125000000000004 0.0 0.0 0.0 0.0 126-127 4.275 0.0 0.0 0.0 0.0 128-129 4.8375 0.0 0.0 0.0 0.0 130-131 5.5 0.0 0.0 0.0 0.0 132-133 6.1 0.0 0.0 0.0 0.0 134-135 6.525 0.0 0.0 0.0 0.0 136-137 6.9375 0.0 0.0 0.0 0.0 138-139 7.425000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGGG 20 3.5877043E-4 108.75 145 >>END_MODULE Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785334 spots for SRR13695483.sra Written 785334 spots for SRR13695483.sra Read 785335 spots for SRR13695483.sra Written 785335 spots for SRR13695483.sra SRR ids: ['SRR13695483.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_hbzl5hkr SRR13695483.sra spots: 15706681 blocks: [[1, 785334], [785335, 1570668], [1570669, 2356002], [2356003, 3141336], [3141337, 3926670], [3926671, 4712004], [4712005, 5497338], [5497339, 6282672], [6282673, 7068006], [7068007, 7853340], [7853341, 8638674], [8638675, 9424008], [9424009, 10209342], [10209343, 10994676], [10994677, 11780010], [11780011, 12565344], [12565345, 13350678], [13350679, 14136012], [14136013, 14921346], [14921347, 15706681]] SRR13695483 file size 5316116 SRR13695483 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695483 SRR13695483_1.fastq SRR13695483_2.fastq Input file: SRR13695483_1.fastq Paired file: SRR13695483_2.fastq trimmed: SRR13695483-trimmed-pair1.fastq, SRR13695483-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 06:24:22 2025 >> started Wed Feb 12 06:24:39 2025 >> done (17.670s) 15706681 read pairs processed; of these: 109 ( 0.00%) short read pairs filtered out after trimming by size control 789 ( 0.01%) empty read pairs filtered out after trimming by size control 15705783 (99.99%) read pairs available; of these: 1560737 ( 9.94%) trimmed read pairs available after processing 14145046 (90.06%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 2 0.00% 20 0 0.00% 21 1 0.00% 22 5 0.00% 23 2 0.00% 24 0 0.00% 25 2 0.00% 26 1 0.00% 27 1 0.00% 28 4 0.00% 29 1 0.00% 30 4 0.00% 31 3 0.00% 32 3 0.00% 33 4 0.00% 34 4 0.00% 35 0 0.00% 36 8 0.00% 37 11 0.00% 38 4 0.00% 39 7 0.00% 40 6 0.00% 41 4 0.00% 42 18 0.00% 43 15 0.00% 44 8 0.00% 45 8 0.00% 46 7 0.00% 47 18 0.00% 48 33 0.00% 49 43 0.00% 50 30 0.00% 51 33 0.00% 52 40 0.00% 53 48 0.00% 54 71 0.00% 55 66 0.00% 56 91 0.00% 57 71 0.00% 58 121 0.00% 59 118 0.00% 60 149 0.00% 61 158 0.00% 62 176 0.00% 63 223 0.00% 64 248 0.00% 65 247 0.00% 66 258 0.00% 67 346 0.00% 68 388 0.00% 69 487 0.00% 70 532 0.00% 71 639 0.00% 72 768 0.00% 73 754 0.00% 74 988 0.01% 75 1049 0.01% 76 1098 0.01% 77 1263 0.01% 78 1402 0.01% 79 1565 0.01% 80 1911 0.01% 81 1996 0.01% 82 2405 0.02% 83 2601 0.02% 84 2998 0.02% 85 3259 0.02% 86 3537 0.02% 87 3865 0.02% 88 4120 0.03% 89 4452 0.03% 90 5081 0.03% 91 5313 0.03% 92 5711 0.04% 93 6358 0.04% 94 6915 0.04% 95 7703 0.05% 96 7912 0.05% 97 8452 0.05% 98 8984 0.06% 99 9654 0.06% 100 10004 0.06% 101 10716 0.07% 102 11162 0.07% 103 11781 0.08% 104 12518 0.08% 105 13454 0.09% 106 13945 0.09% 107 14885 0.09% 108 15571 0.10% 109 16295 0.10% 110 16765 0.11% 111 17517 0.11% 112 17870 0.11% 113 18571 0.12% 114 19404 0.12% 115 20134 0.13% 116 21290 0.14% 117 21839 0.14% 118 22951 0.15% 119 23056 0.15% 120 23926 0.15% 121 24845 0.16% 122 25577 0.16% 123 26095 0.17% 124 27281 0.17% 125 27646 0.18% 126 29116 0.19% 127 29687 0.19% 128 30557 0.19% 129 31433 0.20% 130 32598 0.21% 131 32971 0.21% 132 33715 0.21% 133 34356 0.22% 134 35130 0.22% 135 35564 0.23% 136 36338 0.23% 137 37162 0.24% 138 38012 0.24% 139 39532 0.25% 140 39680 0.25% 141 40367 0.26% 142 41859 0.27% 143 41788 0.27% 144 43512 0.28% 145 44173 0.28% 146 43969 0.28% 147 45300 0.29% 148 46554 0.30% 149 47225 0.30% 150 48157 0.31% 151 14145046 90.06% 15705783 reads passed initial QC criterion=sequence-density sequence-density=0.49 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=19 prefix-density=0.49 prefix-fanout=2.0 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTT criterion=fanout-score sequence-density=0.20 sequence-density-rank=17 fanout-score=7.44 fanout-score-rank=1 prefix-density=0.42 prefix-fanout=3.6 sequence=TTGCAGCCATTCTC criterion=sequence-density sequence-density=0.71 sequence-density-rank=1 fanout-score=2.08 fanout-score-rank=25 prefix-density=0.72 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=29 fanout-score=70.69 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=6.2 sequence=ACAACTTCAAGGGCAGTAGTCTTAAACCATACTCTAAAATCTTCTTATAATTCCAGTTGTAATATTCTGCTAGCATATAATGGCTTCTTCAATGAGCTTGAAGCTGGCCTGTGCCATGCTTGTAGCGATGGTTGTTAGTGCACCACTAGCAGAAGCTGCCATCTCATGTGGCCAGGTGTCAAGCAGCTTGGCACAATGTATAACCTACCTCCAGAAGGGTGGGGCTGTGCCTGCAGCTTGCTGCAGTGGGTTGAAAGGACTTAATTCTGCAGCCACGACCACCGCCGACCGCCAAGGGGTCTGCAACTGTTTGAAATCCTTGGCTGGTAAGATCTCTGGCATCAACTATGGCGTGGCTGCTGGCCTCCCTTCAAAGTGTGGTGTATCCATCTCCTACAAAATCAGTCCTTCCACAGA SRR13695483 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 06:25:18 Started mapping on | Feb 12 06:25:19 Finished on | Feb 12 06:27:13 Mapping speed, Million of reads per hour | 495.97 Number of input reads | 15705783 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 14747777 Uniquely mapped reads % | 93.90% Average mapped length | 296.02 Number of splices: Total | 14442027 Number of splices: Annotated (sjdb) | 14141521 Number of splices: GT/AG | 14155888 Number of splices: GC/AG | 231023 Number of splices: AT/AC | 7688 Number of splices: Non-canonical | 47428 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.90 Insertion rate per base | 0.02% Insertion average length | 2.47 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 402772 % of reads mapped to multiple loci | 2.56% Number of reads mapped to too many loci | 40505 % of reads mapped to too many loci | 0.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.19% % of reads unmapped: other | 0.09% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 555435 555435 555435 N_multimapping 402772 402772 402772 N_noFeature 566783 14411464 780389 N_ambiguous 220204 1402 96417 UnstrandedReadsAssigned:13960790 PositiveStrandReadsAssigned:334911 NegativeStrandReadsAssigned:13870971 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695483 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695483-trimmed-pair1.fastq SRR13695483-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,705,783 reads, 13,886,744 reads pseudoaligned [quant] estimated average fragment length: 258.07 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,021 rounds 52401 SRR13695483.ke.tsv 34699 SRR13695483.se.tsv 87100 total ==> SRR13695483.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1760.93 300 11.5466 Potri.005G024800.1.v4.1 1035 777.93 253 22.0422 Potri.004G059700.1.v4.1 961 704.049 15 1.44399 Potri.007G009000.2.v4.1 1416 1158.93 0 0 Potri.003G141000.2.v4.1 2943 2685.93 695 17.5374 Potri.016G087400.1.v4.1 270 81.8687 676 559.633 Potri.015G069301.1.v4.1 564 317.763 0 0 Potri.010G195200.1.v4.1 1773 1515.93 42 1.87778 Potri.012G127500.1.v4.1 977 719.998 170 16.0027 ==> SRR13695483.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 847 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 179 Potri.001G212900.v4.1 3 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 2 Potri.001G416900.v4.1 1 Potri.001G452600.v4.1 1 SRR13695483 completed mapping pipeline successfully