Starting /dee2/code/volunteer_pipeline.sh SRR13695484 current disk space = 3050339160064 free memory = 1578987216 SRR13695484 SRAfilesize ff784e9b101c54ffbbd4b360ad612375 SRR13695484.sra SRR13695484.sra file validated SRR13695484 is paired end SRR13695484 is conventional basespace SRR13695484 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695484_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5925 37.0 37.0 37.0 37.0 37.0 2 36.29025 37.0 37.0 37.0 37.0 37.0 3 36.6305 37.0 37.0 37.0 37.0 37.0 4 36.561 37.0 37.0 37.0 37.0 37.0 5 36.672 37.0 37.0 37.0 37.0 37.0 6 36.524 37.0 37.0 37.0 37.0 37.0 7 36.5525 37.0 37.0 37.0 37.0 37.0 8 36.645 37.0 37.0 37.0 37.0 37.0 9 36.544 37.0 37.0 37.0 37.0 37.0 10-14 36.5389 37.0 37.0 37.0 37.0 37.0 15-19 36.5606 37.0 37.0 37.0 37.0 37.0 20-24 36.5504 37.0 37.0 37.0 37.0 37.0 25-29 36.4492 37.0 37.0 37.0 37.0 37.0 30-34 36.4605 37.0 37.0 37.0 37.0 37.0 35-39 36.4648 37.0 37.0 37.0 37.0 37.0 40-44 36.4627 37.0 37.0 37.0 37.0 37.0 45-49 36.381600000000006 37.0 37.0 37.0 37.0 37.0 50-54 36.379 37.0 37.0 37.0 37.0 37.0 55-59 36.38399999999999 37.0 37.0 37.0 37.0 37.0 60-64 36.375600000000006 37.0 37.0 37.0 37.0 37.0 65-69 36.37329999999999 37.0 37.0 37.0 37.0 37.0 70-74 36.2941 37.0 37.0 37.0 37.0 37.0 75-79 36.327600000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.288 37.0 37.0 37.0 37.0 37.0 85-89 36.24249999999999 37.0 37.0 37.0 37.0 37.0 90-94 36.21210000000001 37.0 37.0 37.0 37.0 37.0 95-99 36.2036 37.0 37.0 37.0 37.0 37.0 100-104 36.1332 37.0 37.0 37.0 37.0 37.0 105-109 36.134699999999995 37.0 37.0 37.0 37.0 37.0 110-114 36.0842 37.0 37.0 37.0 37.0 37.0 115-119 36.1064 37.0 37.0 37.0 37.0 37.0 120-124 36.047000000000004 37.0 37.0 37.0 37.0 37.0 125-129 36.007099999999994 37.0 37.0 37.0 37.0 37.0 130-134 36.0224 37.0 37.0 37.0 37.0 37.0 135-139 35.9673 37.0 37.0 37.0 37.0 37.0 140-144 35.8667 37.0 37.0 37.0 37.0 37.0 145-149 35.7403 37.0 37.0 37.0 37.0 37.0 150-151 35.579750000000004 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 0.0 24 1.0 25 3.0 26 5.0 27 5.0 28 5.0 29 21.0 30 32.0 31 40.0 32 47.0 33 66.0 34 125.0 35 312.0 36 2965.0 37 372.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.525 12.2 8.25 42.025 2 19.73386894300778 13.65804669846849 36.881747426562896 29.726336931960834 3 17.2 17.625 26.424999999999997 38.75 4 23.35 24.275 25.174999999999997 27.200000000000003 5 23.875 31.35 23.200000000000003 21.575 6 20.474999999999998 35.3 23.025000000000002 21.2 7 15.25 28.125 40.775 15.85 8 17.375 26.974999999999998 33.1 22.55 9 17.5 22.900000000000002 34.875 24.725 10-14 19.595000000000002 29.609999999999996 27.605 23.189999999999998 15-19 19.865 28.139999999999997 28.33 23.665 20-24 20.18 28.825 27.944999999999997 23.05 25-29 19.509999999999998 28.985 28.04 23.465 30-34 20.02 28.87 28.035 23.075000000000003 35-39 19.8 28.38 28.03 23.79 40-44 20.32 28.815 27.815 23.05 45-49 20.39 29.175 27.279999999999998 23.155 50-54 20.150000000000002 28.845 27.935 23.07 55-59 20.349999999999998 28.405 27.755000000000003 23.49 60-64 19.975 29.39 27.229999999999997 23.405 65-69 20.18 28.360000000000003 27.3 24.16 70-74 20.18 27.99 27.98 23.849999999999998 75-79 19.535 28.785 27.839999999999996 23.84 80-84 21.265 28.065 26.875 23.794999999999998 85-89 20.525 29.060000000000002 27.205000000000002 23.21 90-94 21.26 28.59 27.305 22.845 95-99 20.51 28.26 27.605 23.625 100-104 20.375 28.665000000000003 28.055000000000003 22.905 105-109 19.53 28.53 27.99 23.95 110-114 20.435 28.244999999999997 28.244999999999997 23.075000000000003 115-119 21.2 28.475 27.465 22.86 120-124 20.265 28.625 26.745 24.365000000000002 125-129 20.995 28.560000000000002 27.605 22.84 130-134 21.279999999999998 28.439999999999998 27.034999999999997 23.244999999999997 135-139 21.27 28.225 26.26 24.245 140-144 21.685 28.105000000000004 27.195000000000004 23.015 145-149 21.029999999999998 28.810000000000002 26.995 23.165 150-151 21.95 26.5125 27.537499999999998 24.0 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 3.0 16 3.0 17 1.5 18 0.5 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 2.0 25 5.0 26 8.0 27 10.5 28 10.5 29 14.0 30 26.0 31 28.5 32 27.5 33 32.0 34 47.0 35 89.5 36 114.5 37 116.5 38 120.0 39 137.5 40 180.5 41 208.0 42 230.5 43 253.0 44 253.5 45 253.0 46 275.0 47 269.0 48 250.5 49 209.0 50 149.5 51 142.5 52 118.5 53 85.5 54 90.0 55 75.0 56 49.5 57 32.0 58 16.5 59 24.5 60 20.0 61 7.0 62 3.0 63 1.5 64 1.5 65 1.5 66 1.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.42500000000000004 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.875 #Duplication Level Percentage of deduplicated Percentage of total 1 66.29981024667931 43.675000000000004 2 22.23908918406072 29.299999999999997 3 7.020872865275142 13.875000000000002 4 2.9601518026565463 7.8 5 0.9487666034155597 3.125 6 0.3795066413662239 1.5 7 0.11385199240986717 0.525 8 0.03795066413662239 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCCGATTCAGCATCCGAATCCAGAAGCTAAAAACAAAAACAAAGTAGAA 8 0.2 No Hit CCCTTTTGTTATCAACACTAAAACACAATCTTGCATCATTATTCACACTA 7 0.17500000000000002 No Hit GCATAAGTAACCATGTCTGTCATGTAACGACGGGTCATTTCACGTGAGAC 7 0.17500000000000002 No Hit GATTTATTTAAGCTTGACGTAGACTATATATCTATACAGTACAAATTAGG 7 0.17500000000000002 No Hit CTCCCGCACTCATCATGATTGGGTTAATTCCCATCTTGGCAAAGACAAGT 6 0.15 No Hit CGAGTGTATGGCAAGTGTGATGCTGTCATCATTATGGATGCATCATAGGC 6 0.15 No Hit TTTTTTTTTTTTGTTCTTTTCATACATCACATTACAAAATTATAAAAAAT 6 0.15 No Hit GTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTTGG 6 0.15 No Hit ATGCAATACATGCACATACCACATTACATGGTCAATCTGCTAAATACACA 6 0.15 No Hit GCAACTAATAATGATCGAACTTCCGAACTAAGCCTCTCCTCTTCTTTTGT 6 0.15 No Hit GCCAAGAGTATTTCAGATACTGGTTATCACGCCTGCCATATTTTTGGCAA 6 0.15 No Hit GATCATTTAATTATCATCCATCTTTGGGTGTCGAATGATGCAGAAATTAA 6 0.15 No Hit ATCATCACTCCTTGCAGTTGGATCAAAGTTTGCTGCATTTGGATCAGTAC 6 0.15 No Hit CCTTGCACCTAACCAGGAATACCTCTTGAGCTTTCTCAACGTTCTCCTTC 6 0.15 No Hit TGATATTTTTCGAAGTCGTCTGGCTTCTTGCTGAGACCAAAAGGATCGTA 5 0.125 No Hit CAGTCAACTGATTTAAACCCACAATATAAATATTCATCATGAGAGCAGCA 5 0.125 No Hit GGATTCACAAGGAGGAGGAAGTGTTTATGTTTCTGGACAATCACTGGTAC 5 0.125 No Hit GAGGAAAATCAGCATTTTGAGGATATTCGTTTGTGATAACCAGTTCATGT 5 0.125 No Hit GTCCCCAGTAACCATGTACACAATGCACACACCAACTTCAACAATAAGTT 5 0.125 No Hit AGCCCAGCCAATAAAGAACAACTCAACAATGAAGAGTGTGGTGGTGTCAG 5 0.125 No Hit CCTAACCAATATTTTCTTTATATTAATATTCTTTTTAAATTATGGTTGCT 5 0.125 No Hit GCCAAGTATGCCATTGTTATCTTCGAGAACAATTGCATTATGGGTAAGAT 5 0.125 No Hit CTTTTTTAAAGCAGCTTCACATGGGAGGTTGGGATCATCAACAAAAGAGG 5 0.125 No Hit CTCTTTTTGTTGGGTCATTGAATTGTACAGTTTGAGCTCTTCGTTGTTTG 5 0.125 No Hit ATTGGACGGACTACCACCAGTAAGTAGAGGATTGCCCACAGTGACAACAT 5 0.125 No Hit GCTTTTTCAGATCTTCACGCCCAGAGTAAGATGGCAGAACTGGTGCAACT 5 0.125 No Hit GTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCC 5 0.125 No Hit GGAAACTAACAAGAAAATTGAAAATCTGATGAACTCATCAGATCTTAAAA 5 0.125 No Hit CTTAGCTAGGGATTCTTTTTCTAATTGGTTTAGATTTTTTTTTCCAACTT 5 0.125 No Hit GACACACTCTTCCATTTCCTCCCTCTCAGCCACTTTCTCAGGGGATAAGC 5 0.125 No Hit CTCTTTTGATCTTATTGTCGCATACCTTTGAAGTGTCTCCCCATCAAAAG 5 0.125 No Hit GTTGTCGATGGTGGTTTCGGCTGAAGTGAGAAAGCCAATGGTATTATTTA 5 0.125 No Hit CTCAACTTTTCCCTTTTTGAATTCGGCTATTGCCTGAGGTATGTCGGTTG 5 0.125 No Hit GCGTTGGCAAACGGGCACATAATGGAAATGCCATGAGCAGGTATAACGTG 5 0.125 No Hit CCCGAATTCGATCATTAAAACTTCCAATTCCAGTCCCACCAACATTGAAC 5 0.125 No Hit ATCCGCGATTACTAGCGATTCCAACTTCATGTTCCCGAGTTGCAGAGAAC 5 0.125 No Hit ATTCGTTTCGGCTGTGGCAGAAATGTCTCGAGATGTATCAGGGCCTCAAG 5 0.125 No Hit GCATGTTTTGTCGGTAATTAATTTACCTACGAAATCACTGACGGAAATGC 5 0.125 No Hit CTAGAATACACAACGAAGCACAAAGTCTTGGTACCATCAAGGCTATGAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0125 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.037500000000000006 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.0625 0.0 0.0 0.0 0.0 84-85 0.1 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.225 0.0 0.0 0.0 0.0 90-91 0.25 0.0 0.0 0.0 0.0 92-93 0.275 0.0 0.0 0.0 0.0 94-95 0.3 0.0 0.0 0.0 0.0 96-97 0.3375 0.0 0.0 0.0 0.0 98-99 0.55 0.0 0.0 0.0 0.0 100-101 0.7625 0.0 0.0 0.0 0.0 102-103 0.9624999999999999 0.0 0.0 0.0 0.0 104-105 1.2374999999999998 0.0 0.0 0.0 0.0 106-107 1.575 0.0 0.0 0.0 0.0 108-109 1.85 0.0 0.0 0.0 0.0 110-111 2.2 0.0 0.0 0.0 0.0 112-113 2.425 0.0 0.0 0.0 0.0 114-115 2.625 0.0 0.0 0.0 0.0 116-117 3.1125 0.0 0.0 0.0 0.0 118-119 3.5125 0.0 0.0 0.0 0.0 120-121 3.7875 0.0 0.0 0.0 0.0 122-123 4.0375 0.0 0.0 0.0 0.0 124-125 4.5125 0.0 0.0 0.0 0.0 126-127 4.875 0.0 0.0 0.0 0.0 128-129 5.362500000000001 0.0 0.0 0.0 0.0 130-131 6.15 0.0 0.0 0.0 0.0 132-133 6.65 0.0 0.0 0.0 0.0 134-135 7.2125 0.0 0.0 0.0 0.0 136-137 7.7875 0.0 0.0 0.0 0.0 138-139 8.275 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGAATG 10 0.006830828 145.0 4 TTAAGGC 10 0.006830828 145.0 7 TCTTTAA 10 0.006830828 145.0 4 GAAGGAA 10 0.006830828 145.0 2 >>END_MODULE SRR13695484 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695484_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.02175 37.0 37.0 37.0 37.0 37.0 2 36.117 37.0 37.0 37.0 37.0 37.0 3 36.054 37.0 37.0 37.0 37.0 37.0 4 36.0425 37.0 37.0 37.0 37.0 37.0 5 36.146 37.0 37.0 37.0 37.0 37.0 6 36.111 37.0 37.0 37.0 37.0 37.0 7 36.082 37.0 37.0 37.0 37.0 37.0 8 36.1035 37.0 37.0 37.0 37.0 37.0 9 36.121 37.0 37.0 37.0 37.0 37.0 10-14 36.174 37.0 37.0 37.0 37.0 37.0 15-19 36.114 37.0 37.0 37.0 37.0 37.0 20-24 36.086600000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.0304 37.0 37.0 37.0 37.0 37.0 30-34 35.964150000000004 37.0 37.0 37.0 37.0 37.0 35-39 35.94840000000001 37.0 37.0 37.0 37.0 37.0 40-44 35.957950000000004 37.0 37.0 37.0 37.0 37.0 45-49 35.9379 37.0 37.0 37.0 37.0 37.0 50-54 35.89235 37.0 37.0 37.0 37.0 37.0 55-59 35.8697 37.0 37.0 37.0 37.0 37.0 60-64 35.877700000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.8066 37.0 37.0 37.0 37.0 37.0 70-74 35.7198 37.0 37.0 37.0 37.0 37.0 75-79 35.778949999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.818650000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.628249999999994 37.0 37.0 37.0 37.0 37.0 90-94 35.65839999999999 37.0 37.0 37.0 37.0 37.0 95-99 35.684650000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.5312 37.0 37.0 37.0 37.0 37.0 105-109 35.56435 37.0 37.0 37.0 37.0 37.0 110-114 35.491699999999994 37.0 37.0 37.0 37.0 37.0 115-119 35.55155 37.0 37.0 37.0 37.0 37.0 120-124 35.4277 37.0 37.0 37.0 34.6 37.0 125-129 35.441449999999996 37.0 37.0 37.0 34.6 37.0 130-134 35.296800000000005 37.0 37.0 37.0 32.2 37.0 135-139 35.2886 37.0 37.0 37.0 32.2 37.0 140-144 35.2134 37.0 37.0 37.0 29.8 37.0 145-149 35.015699999999995 37.0 37.0 37.0 27.4 37.0 150-151 34.790375 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 2.0 6 1.0 7 0.0 8 0.0 9 0.0 10 1.0 11 0.0 12 0.0 13 0.0 14 1.0 15 3.0 16 1.0 17 2.0 18 1.0 19 2.0 20 0.0 21 2.0 22 4.0 23 3.0 24 4.0 25 12.0 26 7.0 27 14.0 28 15.0 29 20.0 30 28.0 31 59.0 32 70.0 33 103.0 34 261.0 35 723.0 36 2521.0 37 140.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.140848606577954 22.62113984433844 13.457193070549836 30.780818478533767 2 27.55 25.45 30.175 16.825000000000003 3 19.650000000000002 28.849999999999998 31.424999999999997 20.075000000000003 4 21.5 34.975 24.025 19.5 5 26.974999999999998 34.4 22.925 15.7 6 20.549999999999997 39.825 22.575 17.05 7 20.325 21.5 40.075 18.099999999999998 8 19.650000000000002 25.374999999999996 30.875000000000004 24.099999999999998 9 22.475 24.099999999999998 29.175 24.25 10-14 22.415 30.135 26.735 20.715 15-19 23.185 28.16 27.950000000000003 20.705000000000002 20-24 21.944750275247724 28.931037934140726 27.58482634370934 21.539385446902212 25-29 21.97796695042564 28.88833249874812 28.337506259389084 20.796194291437157 30-34 22.02032337187766 27.446563548080295 29.193572608499775 21.33954047154227 35-39 22.785506956260633 28.045240716644983 28.575718146331695 20.59353418076269 40-44 22.88131351053712 28.763077539170045 27.311408119337237 21.0442008309556 45-49 21.916916916916918 28.773773773773776 27.637637637637635 21.67167167167167 50-54 22.283941350147625 28.078867036981435 28.47920732622729 21.157984286643646 55-59 23.189784677015524 27.73660490736104 28.447671507260893 20.625938908362542 60-64 22.960664598138326 27.02432188970073 28.2854569112201 21.729556600940846 65-69 22.861002701891323 28.73011107775443 27.314119883918742 21.094766336435505 70-74 22.503755633450176 28.087130696044067 27.476214321482225 21.932899349023536 75-79 22.53817271589487 27.894868585732162 28.290362953692117 21.27659574468085 80-84 22.827120340255192 27.235426569927444 28.281210908181137 21.656242181636227 85-89 23.253717890941868 28.21591307395724 27.524911121125633 21.005457913975263 90-94 23.408408408408405 28.308308308308312 27.34234234234234 20.94094094094094 95-99 22.779362458089373 28.32407546414452 27.938747935745383 20.957814142020716 100-104 23.44016024036054 27.401101652478715 28.172258387581373 20.986479719579368 105-109 23.52410995944119 27.975564568624506 28.311051023984778 20.189274447949526 110-114 22.965669102191974 27.995195676108498 28.475628065258736 20.563507156440796 115-119 23.626896310018523 27.762479347118614 27.366945376257952 21.243678966604918 120-124 24.10615923885829 27.476214321482225 28.44266399599399 19.974962443665497 125-129 23.8648310387985 27.519399249061326 27.804755944931163 20.811013767209012 130-134 24.814703525641026 28.435496794871796 26.802884615384613 19.946915064102562 135-139 24.69704556835253 27.871807711567353 27.42113169754632 20.0100150225338 140-144 25.558002201981783 26.794114703232907 27.870083074767287 19.777800020018017 145-149 25.778668002003002 27.025538307461193 27.46119178768152 19.734601902854283 150-151 26.53061224489796 28.208338550143985 26.66833604607487 18.592713158883186 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.5 8 1.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.5 19 5.0 20 6.5 21 2.5 22 1.0 23 1.5 24 5.5 25 8.5 26 7.0 27 9.0 28 15.0 29 19.0 30 17.0 31 21.5 32 33.0 33 47.5 34 57.0 35 70.5 36 95.0 37 121.0 38 153.0 39 172.0 40 184.5 41 198.5 42 228.5 43 281.0 44 277.0 45 273.0 46 275.5 47 232.5 48 197.0 49 183.5 50 163.0 51 132.0 52 116.0 53 91.5 54 71.5 55 47.5 56 38.0 57 42.5 58 23.0 59 9.5 60 14.0 61 13.0 62 8.0 63 4.0 64 1.0 65 4.0 66 3.5 67 0.5 68 0.5 69 0.5 70 1.0 71 1.0 72 1.0 73 1.5 74 0.5 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42500000000000004 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.09 25-29 0.15 30-34 0.11499999999999999 35-39 0.09 40-44 0.11499999999999999 45-49 0.1 50-54 0.08499999999999999 55-59 0.15 60-64 0.09 65-69 0.06999999999999999 70-74 0.15 75-79 0.125 80-84 0.075 85-89 0.145 90-94 0.1 95-99 0.08499999999999999 100-104 0.15 105-109 0.145 110-114 0.09 115-119 0.135 120-124 0.15 125-129 0.125 130-134 0.16 135-139 0.15 140-144 0.09 145-149 0.15 150-151 0.1625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.60000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 67.37987987987988 44.875 2 21.734234234234233 28.95 3 6.418918918918918 12.825000000000001 4 3.040540540540541 8.1 5 0.8633633633633633 2.875 6 0.487987987987988 1.95 7 0.0 0.0 8 0.03753753753753754 0.2 9 0.03753753753753754 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 9 0.22499999999999998 No Hit GGTCACTCCACCGCTGGCGTGGGAAGACGGAGGGGAAGAGGACATTGCAG 8 0.2 No Hit GTAAGAGATGGCTTCCTCTGTGATTTCATCGGCGGCCGTTGCCACAGTTA 6 0.15 No Hit AGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 6 0.15 No Hit GGGACTTGCCACCACTCCAGCTTGTTTTATGAGTACTTTGTTAACTCCAT 6 0.15 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 6 0.15 No Hit TCATGATCCCCGATTTCCTCCTCCTCCTCTGTATTTTAGGCAGCCAAATG 6 0.15 No Hit GTCAGGTTTTGGTCTGTACTTTTTGTCTAGTGATGCTTTTGTTTTTGCAA 6 0.15 No Hit ATGGTAGAGTAGCATCCTTAGCGTTGCTGGTTTACTTTCCTAACAATCCT 6 0.15 No Hit AATTTGGAGGAAATACTGTGAATGCGTTTTATTATCCAGAAGAGAAGTAT 6 0.15 No Hit AAACCACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTT 6 0.15 No Hit GCTTTCTCCAATTTCATTTACATTGACAAAAACCCTTTAACAAAACCTTA 6 0.15 No Hit ACAACATGGATGGTTTCTACATTGCTCCTGCTTTCATGGACAAGCTTGTT 6 0.15 No Hit ATTGGGCTTTGGTGATCGGGCCAGTGACCTTATCTGGTTCTCAGATAAGC 6 0.15 No Hit CTGCATTGGAATATTCAAGAGTGACAAAATTCCCAAGGAAGATATTGTCA 6 0.15 No Hit TTTTTATTTTCAGGTAATCTATTTTTATAAGAAGTTTACAACTCCTTAAA 5 0.125 No Hit TGGAGAGCCAGGAGTTGGCAAAACCGCAGTTGCTGAAGGTTTGGCCCAAA 5 0.125 No Hit CATCATGGTGTTGCTGGTGATTGGTGCAATTCTATATATAAATCCATATT 5 0.125 No Hit ATTTGTCAAGAGACAACCAGAGATTCACCTTTTTGCGGCGCGCTTCAAAG 5 0.125 No Hit TGAATGGTCCCCATCCGGTGATGTCTATGCTGGGGGCAAGACTGGCTTGC 5 0.125 No Hit GTTTAGAAAAGTCACGGAGAGAGCATCGACAAGACGACGCAACCTCATCC 5 0.125 No Hit AATGACCAACAGCTGCGAGCAACAGTAAATTTGCCCAAGGGAACAGGACA 5 0.125 No Hit TTCAGTTGCAAGGCTAGGTTCTCTTATTCAAATCAAACTCGGGAGTAACA 5 0.125 No Hit CGATTGCTATTGGGTGTGAAGCAATTGAGACACAATCGACATGGCCGCCC 5 0.125 No Hit AGGATTTTTAAATTTTTAGCAATTGCAACTTCATTTTTTTCATACGAAAC 5 0.125 No Hit CAGTGACCGCGCTACGCAGAACCGTTCCACCAGCTGTACCAGGCATTGTG 5 0.125 No Hit GTCGGTGAAAAATCGGTTCTTGATGTTCCGGGTCAACCCGAAATGTTACC 5 0.125 No Hit CGGATGATCATTGATGATATGTTAAACTGGGCTGGTAATTATAAGGTTGA 5 0.125 No Hit CTTTGGTTTCGATCCTCTAGGTCTTGGATCTGATCCTGAAACTCTGAGAT 5 0.125 No Hit CCTTATCTTGCAGGTGACGACGACGTCGAGTTGGCGGCGGAGAAAGACTC 5 0.125 No Hit ATTTCTAAGGGGAACCAATTTGATGGAAGATGCTCGAGGAATGGCATCGC 5 0.125 No Hit CTTTCATTAACAAAAACGAATTAACGCAGCACATAAACATGTTGACGAGG 5 0.125 No Hit TATAAATTCAACAAAAGTTTAATGATCATTGTATGCCATTTCCAACTTTT 5 0.125 No Hit GCAATATTTCATCTTATAGCTCTGAATTTTCTTGAGCTTCTCAGCGTGTC 5 0.125 No Hit TGATGACTGTAGAAATGTCAATTGTTGGAAGCTTGTGCTTGTTGGCCAGT 5 0.125 No Hit CATATCTTTCCATCTCAATATCAAACCCTTCCTTTTCTGGAAAAGACATG 5 0.125 No Hit GTTCGAGCAGCTTCCTTCTCCACCATAGATGACTTGGTGGCCTTTGTAAA 5 0.125 No Hit CGCTCCTAAACCAAAGCCTTCTGCTGTTTCACCAGCTGATGTGGAGCTCG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0125 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.037500000000000006 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.0625 0.0 0.0 0.0 0.0 84-85 0.1 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.225 0.0 0.0 0.0 0.0 90-91 0.25 0.0 0.0 0.0 0.0 92-93 0.275 0.0 0.0 0.0 0.0 94-95 0.3 0.0 0.0 0.0 0.0 96-97 0.3375 0.0 0.0 0.0 0.0 98-99 0.55 0.0 0.0 0.0 0.0 100-101 0.75 0.0 0.0 0.0 0.0 102-103 0.9375 0.0 0.0 0.0 0.0 104-105 1.2125 0.0 0.0 0.0 0.0 106-107 1.55 0.0 0.0 0.0 0.0 108-109 1.8250000000000002 0.0 0.0 0.0 0.0 110-111 2.1875 0.0 0.0 0.0 0.0 112-113 2.45 0.0 0.0 0.0 0.0 114-115 2.6500000000000004 0.0 0.0 0.0 0.0 116-117 3.05 0.0 0.0 0.0 0.0 118-119 3.4375 0.0 0.0 0.0 0.0 120-121 3.7125 0.0 0.0 0.0 0.0 122-123 3.9625000000000004 0.0 0.0 0.0 0.0 124-125 4.4125 0.0 0.0 0.0 0.0 126-127 4.775 0.0 0.0 0.0 0.0 128-129 5.262499999999999 0.0 0.0 0.0 0.0 130-131 6.074999999999999 0.0 0.0 0.0 0.0 132-133 6.575 0.0 0.0 0.0 0.0 134-135 7.1375 0.0 0.0 0.0 0.0 136-137 7.725 0.0 0.0 0.0 0.0 138-139 8.225 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCACCT 10 0.006830828 145.0 7 GGGCAAT 10 0.006830828 145.0 7 CTGGGCA 10 0.006830828 145.0 5 GGATTCA 10 0.006830828 145.0 4 CGGATTC 10 0.006830828 145.0 3 GCGGATT 10 0.006830828 145.0 2 GCAATGG 10 0.006830828 145.0 9 AGCGGAT 10 0.006830828 145.0 1 TACTGGG 10 0.006830828 145.0 3 GATTCAC 10 0.006830828 145.0 5 TGGGCAA 10 0.006830828 145.0 6 ACTGGGC 10 0.006830828 145.0 4 ATTCACC 10 0.006830828 145.0 6 >>END_MODULE Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795441 spots for SRR13695484.sra Written 795441 spots for SRR13695484.sra Read 795451 spots for SRR13695484.sra Written 795451 spots for SRR13695484.sra SRR ids: ['SRR13695484.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_jpt8gvmf SRR13695484.sra spots: 15908830 blocks: [[1, 795441], [795442, 1590882], [1590883, 2386323], [2386324, 3181764], [3181765, 3977205], [3977206, 4772646], [4772647, 5568087], [5568088, 6363528], [6363529, 7158969], [7158970, 7954410], [7954411, 8749851], [8749852, 9545292], [9545293, 10340733], [10340734, 11136174], [11136175, 11931615], [11931616, 12727056], [12727057, 13522497], [13522498, 14317938], [14317939, 15113379], [15113380, 15908830]] SRR13695484 file size 5384816 SRR13695484 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695484 SRR13695484_1.fastq SRR13695484_2.fastq Input file: SRR13695484_1.fastq Paired file: SRR13695484_2.fastq trimmed: SRR13695484-trimmed-pair1.fastq, SRR13695484-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 06:21:55 2025 >> started Wed Feb 12 06:22:11 2025 >> done (16.534s) 15908830 read pairs processed; of these: 122 ( 0.00%) short read pairs filtered out after trimming by size control 1151 ( 0.01%) empty read pairs filtered out after trimming by size control 15907557 (99.99%) read pairs available; of these: 1811770 (11.39%) trimmed read pairs available after processing 14095787 (88.61%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 0 0.00% 20 3 0.00% 21 5 0.00% 22 1 0.00% 23 4 0.00% 24 0 0.00% 25 0 0.00% 26 0 0.00% 27 3 0.00% 28 3 0.00% 29 4 0.00% 30 1 0.00% 31 7 0.00% 32 5 0.00% 33 2 0.00% 34 4 0.00% 35 2 0.00% 36 5 0.00% 37 3 0.00% 38 2 0.00% 39 12 0.00% 40 5 0.00% 41 6 0.00% 42 13 0.00% 43 7 0.00% 44 14 0.00% 45 7 0.00% 46 15 0.00% 47 21 0.00% 48 24 0.00% 49 36 0.00% 50 35 0.00% 51 39 0.00% 52 46 0.00% 53 50 0.00% 54 56 0.00% 55 46 0.00% 56 89 0.00% 57 89 0.00% 58 107 0.00% 59 110 0.00% 60 158 0.00% 61 214 0.00% 62 210 0.00% 63 218 0.00% 64 237 0.00% 65 290 0.00% 66 351 0.00% 67 397 0.00% 68 475 0.00% 69 481 0.00% 70 674 0.00% 71 793 0.00% 72 883 0.01% 73 1050 0.01% 74 1163 0.01% 75 1291 0.01% 76 1477 0.01% 77 1594 0.01% 78 1844 0.01% 79 2115 0.01% 80 2283 0.01% 81 2586 0.02% 82 3066 0.02% 83 3358 0.02% 84 3747 0.02% 85 4395 0.03% 86 4428 0.03% 87 5025 0.03% 88 5269 0.03% 89 5639 0.04% 90 6339 0.04% 91 6985 0.04% 92 7307 0.05% 93 7923 0.05% 94 8782 0.06% 95 9549 0.06% 96 10135 0.06% 97 10611 0.07% 98 11315 0.07% 99 11840 0.07% 100 12496 0.08% 101 12929 0.08% 102 13545 0.09% 103 14682 0.09% 104 15246 0.10% 105 16418 0.10% 106 17031 0.11% 107 17744 0.11% 108 18707 0.12% 109 19423 0.12% 110 19599 0.12% 111 20467 0.13% 112 21332 0.13% 113 21908 0.14% 114 22956 0.14% 115 24031 0.15% 116 24994 0.16% 117 26343 0.17% 118 26310 0.17% 119 27679 0.17% 120 28362 0.18% 121 28934 0.18% 122 30151 0.19% 123 30706 0.19% 124 31618 0.20% 125 31860 0.20% 126 34089 0.21% 127 34626 0.22% 128 35358 0.22% 129 36015 0.23% 130 37636 0.24% 131 37662 0.24% 132 38191 0.24% 133 38807 0.24% 134 39830 0.25% 135 40650 0.26% 136 41965 0.26% 137 43035 0.27% 138 43570 0.27% 139 45112 0.28% 140 45482 0.29% 141 45692 0.29% 142 47151 0.30% 143 47174 0.30% 144 48347 0.30% 145 49340 0.31% 146 49471 0.31% 147 51583 0.32% 148 51799 0.33% 149 52349 0.33% 150 53986 0.34% 151 14095787 88.61% 15907557 reads passed initial QC criterion=sequence-density sequence-density=0.45 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=24 prefix-density=0.46 prefix-fanout=1.9 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=26 fanout-score=510.55 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=18.1 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGGGTATGAATGTGTTCTCTGT criterion=sequence-density sequence-density=0.56 sequence-density-rank=1 fanout-score=2.20 fanout-score-rank=28 prefix-density=0.57 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.16 sequence-density-rank=19 fanout-score=15.41 fanout-score-rank=1 prefix-density=0.60 prefix-fanout=4.1 sequence=AATGGCAGCCTCAGTTATGGCTTCATT SRR13695484 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 06:22:52 Started mapping on | Feb 12 06:22:52 Finished on | Feb 12 06:24:30 Mapping speed, Million of reads per hour | 584.36 Number of input reads | 15907557 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 15047597 Uniquely mapped reads % | 94.59% Average mapped length | 295.14 Number of splices: Total | 15140209 Number of splices: Annotated (sjdb) | 14809156 Number of splices: GT/AG | 14820565 Number of splices: GC/AG | 258216 Number of splices: AT/AC | 8113 Number of splices: Non-canonical | 53315 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.86 Insertion rate per base | 0.02% Insertion average length | 2.46 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 374926 % of reads mapped to multiple loci | 2.36% Number of reads mapped to too many loci | 51233 % of reads mapped to too many loci | 0.32% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.63% % of reads unmapped: other | 0.10% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 485247 485247 485247 N_multimapping 374926 374926 374926 N_noFeature 612700 14699459 829790 N_ambiguous 226815 1586 94563 UnstrandedReadsAssigned:14208082 PositiveStrandReadsAssigned:346552 NegativeStrandReadsAssigned:14123244 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695484 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695484-trimmed-pair1.fastq SRR13695484-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,907,557 reads, 14,164,983 reads pseudoaligned [quant] estimated average fragment length: 248.301 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,168 rounds 52401 SRR13695484.ke.tsv 34699 SRR13695484.se.tsv 87100 total ==> SRR13695484.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1770.7 534 19.8072 Potri.005G024800.1.v4.1 1035 787.699 265 22.096 Potri.004G059700.1.v4.1 961 713.791 11 1.01216 Potri.007G009000.2.v4.1 1416 1168.7 0 0 Potri.003G141000.2.v4.1 2943 2695.7 892 21.733 Potri.016G087400.1.v4.1 270 83.4074 411 323.642 Potri.015G069301.1.v4.1 564 325.081 0 0 Potri.010G195200.1.v4.1 1773 1525.7 187.905 8.08902 Potri.012G127500.1.v4.1 977 729.73 93 8.37043 ==> SRR13695484.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 133 Potri.001G233950.v4.1 1 Potri.001G122700.v4.1 193 Potri.001G212900.v4.1 12 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 24 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695484 completed mapping pipeline successfully