Starting /dee2/code/volunteer_pipeline.sh SRR13695485 current disk space = 3050308255744 free memory = 1299841536 SRR13695485 SRAfilesize ef281a822946f440cd726ef6486be28b SRR13695485.sra SRR13695485.sra file validated SRR13695485 is paired end SRR13695485 is conventional basespace SRR13695485 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695485_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5275 37.0 37.0 37.0 37.0 37.0 2 36.2535 37.0 37.0 37.0 37.0 37.0 3 36.5435 37.0 37.0 37.0 37.0 37.0 4 36.426 37.0 37.0 37.0 37.0 37.0 5 36.4965 37.0 37.0 37.0 37.0 37.0 6 36.5775 37.0 37.0 37.0 37.0 37.0 7 36.481 37.0 37.0 37.0 37.0 37.0 8 36.4805 37.0 37.0 37.0 37.0 37.0 9 36.5175 37.0 37.0 37.0 37.0 37.0 10-14 36.5384 37.0 37.0 37.0 37.0 37.0 15-19 36.48020000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.4661 37.0 37.0 37.0 37.0 37.0 25-29 36.4258 37.0 37.0 37.0 37.0 37.0 30-34 36.41 37.0 37.0 37.0 37.0 37.0 35-39 36.376999999999995 37.0 37.0 37.0 37.0 37.0 40-44 36.3726 37.0 37.0 37.0 37.0 37.0 45-49 36.3222 37.0 37.0 37.0 37.0 37.0 50-54 36.331599999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.308299999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.356 37.0 37.0 37.0 37.0 37.0 65-69 36.3007 37.0 37.0 37.0 37.0 37.0 70-74 36.303399999999996 37.0 37.0 37.0 37.0 37.0 75-79 36.2581 37.0 37.0 37.0 37.0 37.0 80-84 36.1484 37.0 37.0 37.0 37.0 37.0 85-89 36.1494 37.0 37.0 37.0 37.0 37.0 90-94 36.1031 37.0 37.0 37.0 37.0 37.0 95-99 36.1046 37.0 37.0 37.0 37.0 37.0 100-104 36.0561 37.0 37.0 37.0 37.0 37.0 105-109 36.04600000000001 37.0 37.0 37.0 37.0 37.0 110-114 36.0319 37.0 37.0 37.0 37.0 37.0 115-119 36.0265 37.0 37.0 37.0 37.0 37.0 120-124 35.928200000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.89110000000001 37.0 37.0 37.0 37.0 37.0 130-134 35.895300000000006 37.0 37.0 37.0 37.0 37.0 135-139 35.7687 37.0 37.0 37.0 37.0 37.0 140-144 35.576299999999996 37.0 37.0 37.0 37.0 37.0 145-149 35.4282 37.0 37.0 37.0 37.0 37.0 150-151 35.23725 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 0.0 22 0.0 23 1.0 24 2.0 25 2.0 26 9.0 27 8.0 28 17.0 29 24.0 30 32.0 31 45.0 32 56.0 33 74.0 34 145.0 35 319.0 36 2938.0 37 327.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.025 11.725 7.175 42.075 2 18.395989974937343 12.982456140350877 39.22305764411028 29.398496240601503 3 17.599999999999998 16.3 27.55 38.550000000000004 4 23.075000000000003 24.2 23.25 29.475 5 23.474999999999998 31.4 25.6 19.525000000000002 6 19.175 34.475 25.124999999999996 21.224999999999998 7 15.25 25.674999999999997 41.175 17.9 8 17.925 26.325 32.625 23.125 9 16.825000000000003 24.6 35.15 23.425 10-14 20.175 29.26 27.6 22.965 15-19 19.685 27.925 28.215 24.175 20-24 20.119999999999997 28.51 28.29 23.080000000000002 25-29 20.015 28.4 27.800000000000004 23.785 30-34 20.549999999999997 28.7 27.11 23.64 35-39 19.285 29.099999999999998 27.46 24.154999999999998 40-44 20.965 28.71 27.700000000000003 22.625 45-49 20.225 28.565 27.750000000000004 23.46 50-54 20.330000000000002 29.475 27.544999999999998 22.650000000000002 55-59 20.4 28.205000000000002 27.855 23.54 60-64 20.335 28.625 28.415000000000003 22.625 65-69 19.68 28.395 27.515 24.41 70-74 20.32 28.375 27.950000000000003 23.355 75-79 20.465 28.235 28.155 23.145 80-84 19.82 28.125 28.475 23.580000000000002 85-89 19.919999999999998 29.154999999999998 28.01 22.915 90-94 20.064999999999998 29.544999999999998 27.150000000000002 23.24 95-99 20.724999999999998 29.020000000000003 27.43 22.825 100-104 20.51 29.609999999999996 27.425 22.455 105-109 20.549999999999997 27.644999999999996 27.965 23.84 110-114 20.880000000000003 28.349999999999998 27.73 23.04 115-119 20.419999999999998 27.975 27.625 23.98 120-124 20.855 28.585 26.575 23.985 125-129 20.13 28.95 28.01 22.91 130-134 20.43 28.595 27.465 23.51 135-139 21.55 28.895 26.625 22.93 140-144 21.19 28.389999999999997 26.279999999999998 24.14 145-149 21.775 28.415000000000003 26.25 23.56 150-151 21.75 28.6625 26.2625 23.325000000000003 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.5 20 1.5 21 2.0 22 2.5 23 1.5 24 1.0 25 0.5 26 2.0 27 9.5 28 13.5 29 12.5 30 21.5 31 35.0 32 33.0 33 33.5 34 51.0 35 64.5 36 83.0 37 122.5 38 149.5 39 170.0 40 206.5 41 233.5 42 247.5 43 265.0 44 258.0 45 254.0 46 266.5 47 235.0 48 216.5 49 204.5 50 169.5 51 143.0 52 120.0 53 94.5 54 65.0 55 42.0 56 37.0 57 35.0 58 20.5 59 17.5 60 18.0 61 11.0 62 9.5 63 8.5 64 5.0 65 2.5 66 1.0 67 0.5 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.25 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.525 #Duplication Level Percentage of deduplicated Percentage of total 1 69.86501276906239 47.875 2 19.737322145202484 27.05 3 6.785844582269244 13.950000000000001 4 2.4443633710324697 6.7 5 0.8391098139365194 2.875 6 0.07296607077708865 0.3 7 0.21889821233126594 1.05 8 0.036483035388544326 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCAGGCTCATGAAGTTCTTGGAGATGTGAACAACAAGCTTGTCCATGAAA 8 0.2 No Hit CTTTATCTATTTCATAGACTGTAATATTAACCCCATCAATGTTTGATAAT 7 0.17500000000000002 No Hit TGGCTATTTACGAGAGGCTTCAGTTTTTGCTGATTCTGATGTGAACATAA 7 0.17500000000000002 No Hit CTCAGCTACGGCACCTTCCTTCCTCCAGTCAACGGAATCCGGCAAAGAGT 7 0.17500000000000002 No Hit GTGAGGTTTGAGGTTTTTGATAAGGATGTCCTCGTGTTATCTGGTGTTTT 7 0.17500000000000002 No Hit CTTACAAGTTACTGTGGACCACAAGCTTTTGAGTCTTATGCATGATGACA 7 0.17500000000000002 No Hit ATCACCATCACTAATCGACCCACCACTATTAATTCCATCACCCACATTTT 7 0.17500000000000002 No Hit ACCAATTTTCACTTCACCTTGGTTTCCATTAACAAAGATGTTGTCACATT 6 0.15 No Hit ATCAATCTTAACCATAATGTTTGAGTAGGTGGACAAGATCGCTTCTGCAT 6 0.15 No Hit ATCCAGACATTAGAAATGGTTCCCTGGAATTCTTATGAACAATCTTGCTT 5 0.125 No Hit GCTAAACCTGCCTCTCTCTCTAAGCAAAAGAGACGGGTTTTTTTCATCTA 5 0.125 No Hit GTGGAGAAGCAGGTCAATAATGAAGACTACCAATAGTAACCAAAAAATAT 5 0.125 No Hit CAGTATCAATTGAGTCAGTACTTGAACACTTGTCAAAAGAAGCCTTTTGA 5 0.125 No Hit ACCTGTCTTGAGGACCACAAGGGATCCGGATTATATAGGCTCCACAACTA 5 0.125 No Hit GCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTT 5 0.125 No Hit GTGAAATTAAGAGTTTACTTCATGTGGCACCGTCGCGTTCTCTACCAAGG 5 0.125 No Hit CCCCAGCCAATGTCATCAGCCTTTCAGAGTAAATCTTCCATGTATATCTT 5 0.125 No Hit TGGCACATACTAAATTAACCATACAGGTATTCTTTTCAGTCACAAGGGCT 5 0.125 No Hit TATCACCCTCCTCCAATGGAGGATCTGACTCTCGAGTAACATAGATGGAA 5 0.125 No Hit CTAGTTTCTTGCAACCTCTTGAGCGGACTCAAACTATGCCAAGGTACAGT 5 0.125 No Hit AAGAGAAATATCTACACCAACTTGAGCGTGGAAAACAGATTGTTGTGAAG 5 0.125 No Hit TGCAGAAACTATCTTGAGAATAACATGTATGAAAAATGGGTTCTATCTAT 5 0.125 No Hit CTGTTGGAATCTTGCGAATCAACATCACAGATATTTTCAATGTTCTCCAA 5 0.125 No Hit GTTCTCGCGACGAACTTCCTCCTCCTCCTCAGGTGTAAAGTCATTCTTGA 5 0.125 No Hit TCCATTTGTACATGTGAGACTAGCAGTTCAAGTTGCTAGTGCTGGGAATG 5 0.125 No Hit GCTATCCAATGCATGGCCTGGTTGAAAACACCTGTTGGTAGAGCAGAAGT 5 0.125 No Hit GCATTTTCCCGCGGGCACTGAAGAGAATGTTGTTCAACTTTATCCATGCT 5 0.125 No Hit GCCAGCCAGAAACTACAACAAAAGTACACAACCAAATGCCGTTATTTTGA 5 0.125 No Hit CTCAAAGCACAATTTCTATCAATACTGTTACAATAACAAAGCAGTCAAAT 5 0.125 No Hit GTCCATAAGACCACTTGGCCACAATTGTGCAAGCTCCACATACAACTTTC 5 0.125 No Hit GTATAAGATATCATGACCAATAAAATGGAAAACCTTGGTCTGCCCCCACA 5 0.125 No Hit CCTCTATTTCCTTCACCAACAACATTATGGTTCCCATTGGAGTGTCCATT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.36250000000000004 0.0 0.0 0.0 0.0 86-87 0.4375 0.0 0.0 0.0 0.0 88-89 0.525 0.0 0.0 0.0 0.0 90-91 0.625 0.0 0.0 0.0 0.0 92-93 0.7625 0.0 0.0 0.0 0.0 94-95 0.9249999999999999 0.0 0.0 0.0 0.0 96-97 1.125 0.0 0.0 0.0 0.0 98-99 1.3250000000000002 0.0 0.0 0.0 0.0 100-101 1.45 0.0 0.0 0.0 0.0 102-103 1.6375000000000002 0.0 0.0 0.0 0.0 104-105 1.8625 0.0 0.0 0.0 0.0 106-107 1.9 0.0 0.0 0.0 0.0 108-109 2.15 0.0 0.0 0.0 0.0 110-111 2.4625 0.0 0.0 0.0 0.0 112-113 2.5999999999999996 0.0 0.0 0.0 0.0 114-115 2.9 0.0 0.0 0.0 0.0 116-117 3.2625 0.0 0.0 0.0 0.0 118-119 3.5999999999999996 0.0 0.0 0.0 0.0 120-121 4.15 0.0 0.0 0.0 0.0 122-123 4.3875 0.0 0.0 0.0 0.0 124-125 4.925 0.0 0.0 0.0 0.0 126-127 5.45 0.0 0.0 0.0 0.0 128-129 6.25 0.0 0.0 0.0 0.0 130-131 6.925 0.0 0.0 0.0 0.0 132-133 7.5125 0.0 0.0 0.0 0.0 134-135 8.3375 0.0 0.0 0.0 0.0 136-137 9.0875 0.0 0.0 0.0 0.0 138-139 9.6625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAACAG 10 0.006830828 145.0 1 CAGCTGC 10 0.006830828 145.0 5 CGTCTGA 40 0.005621335 54.375 145 >>END_MODULE SRR13695485 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695485_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.17525 37.0 37.0 37.0 37.0 37.0 2 36.0665 37.0 37.0 37.0 37.0 37.0 3 36.005 37.0 37.0 37.0 37.0 37.0 4 36.13 37.0 37.0 37.0 37.0 37.0 5 36.1825 37.0 37.0 37.0 37.0 37.0 6 36.14 37.0 37.0 37.0 37.0 37.0 7 36.206 37.0 37.0 37.0 37.0 37.0 8 36.279 37.0 37.0 37.0 37.0 37.0 9 36.3155 37.0 37.0 37.0 37.0 37.0 10-14 36.2507 37.0 37.0 37.0 37.0 37.0 15-19 36.2469 37.0 37.0 37.0 37.0 37.0 20-24 36.1995 37.0 37.0 37.0 37.0 37.0 25-29 36.16330000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.12285000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.0803 37.0 37.0 37.0 37.0 37.0 40-44 36.0862 37.0 37.0 37.0 37.0 37.0 45-49 36.08515 37.0 37.0 37.0 37.0 37.0 50-54 36.08284999999999 37.0 37.0 37.0 37.0 37.0 55-59 36.0306 37.0 37.0 37.0 37.0 37.0 60-64 35.9389 37.0 37.0 37.0 37.0 37.0 65-69 35.9979 37.0 37.0 37.0 37.0 37.0 70-74 35.8705 37.0 37.0 37.0 37.0 37.0 75-79 35.935500000000005 37.0 37.0 37.0 37.0 37.0 80-84 35.95595 37.0 37.0 37.0 37.0 37.0 85-89 35.841350000000006 37.0 37.0 37.0 37.0 37.0 90-94 35.80460000000001 37.0 37.0 37.0 37.0 37.0 95-99 35.80735 37.0 37.0 37.0 37.0 37.0 100-104 35.8302 37.0 37.0 37.0 37.0 37.0 105-109 35.81365000000001 37.0 37.0 37.0 37.0 37.0 110-114 35.682100000000005 37.0 37.0 37.0 37.0 37.0 115-119 35.6756 37.0 37.0 37.0 37.0 37.0 120-124 35.6459 37.0 37.0 37.0 37.0 37.0 125-129 35.67405000000001 37.0 37.0 37.0 37.0 37.0 130-134 35.4964 37.0 37.0 37.0 37.0 37.0 135-139 35.4813 37.0 37.0 37.0 37.0 37.0 140-144 35.347500000000004 37.0 37.0 37.0 34.6 37.0 145-149 35.22280000000001 37.0 37.0 37.0 29.8 37.0 150-151 34.999 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.0 14 2.0 15 0.0 16 0.0 17 1.0 18 0.0 19 1.0 20 2.0 21 3.0 22 2.0 23 2.0 24 4.0 25 5.0 26 6.0 27 12.0 28 15.0 29 19.0 30 37.0 31 40.0 32 68.0 33 111.0 34 206.0 35 582.0 36 2689.0 37 190.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.023779724655824 20.30037546933667 12.015018773466833 28.660826032540676 2 24.775 28.175 31.775 15.275 3 19.775000000000002 28.4 30.349999999999998 21.475 4 24.45 33.225 23.5 18.825 5 25.650000000000002 36.3 22.650000000000002 15.4 6 21.6 37.8 22.625 17.974999999999998 7 19.8 21.925 39.25 19.025 8 20.9 26.924999999999997 28.525 23.65 9 20.95 24.5 33.324999999999996 21.224999999999998 10-14 22.29 30.48 26.479999999999997 20.75 15-19 23.53 28.555000000000003 27.139999999999997 20.775 20-24 22.696809042712815 28.83865159547864 27.91337401220366 20.55116534960488 25-29 22.811405702851424 27.123561780890444 29.119559779889947 20.945472736368185 30-34 22.878007302555893 28.064822687940776 28.354924223478218 20.70224578602511 35-39 21.876562968890667 28.00340102030609 28.79863959187756 21.32139641892568 40-44 22.68407362945178 27.951180472188874 28.9765906362545 20.388155262104842 45-49 22.442854999249736 28.439953983894362 28.094833191617063 21.02235782523883 50-54 21.770442610652662 27.596899224806204 29.367341835458866 21.26531632908227 55-59 22.846423211605803 27.5687843921961 28.629314657328663 20.955477738869437 60-64 22.131639491847555 28.163449034710414 28.703611083324997 21.001300390117038 65-69 22.414482896579315 28.105621124224843 28.230646129225846 21.249249849969996 70-74 23.271635817908955 28.729364682341167 27.813906953476735 20.185092546273136 75-79 22.158863545418168 28.201280512204878 28.331332533013203 21.308523409363744 80-84 23.10577644411103 28.91722930732683 27.426856714178545 20.550137534383595 85-89 23.255464959231652 28.137661947876545 28.317742984342953 20.289130108548846 90-94 22.891867560268082 27.928378513554065 28.4185255576673 20.761228368510555 95-99 24.06101525381345 27.41185296324081 27.911977994498628 20.615153788447113 100-104 23.461730865432717 28.344172086043024 27.648824412206103 20.54527263631816 105-109 23.50057525886649 28.4828172677705 27.587414336451406 20.42919313691161 110-114 22.966890067020106 28.06842052615785 28.48854656396919 20.476142842852855 115-119 24.199679871948778 28.45138055222089 27.490996398559425 19.857943177270908 120-124 24.522261130565283 27.68384192096048 27.893946973486745 19.899949974987493 125-129 24.215897153719173 28.4027812515632 27.36231304086839 20.019008553849233 130-134 24.477238619309656 27.263631815907953 27.34367183591796 20.915457728864432 135-139 24.947473736868435 28.23911955977989 27.688844422211105 19.12456228114057 140-144 25.982794838451532 27.35820746223867 26.95308592577773 19.70591177353206 145-149 25.642821410705352 27.65382691345673 26.68334167083542 20.0200100050025 150-151 26.76338169084542 27.71385692846423 27.388694347173587 18.13406703351676 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.5 19 1.5 20 1.0 21 0.0 22 0.5 23 1.5 24 2.5 25 2.5 26 2.5 27 8.0 28 17.5 29 15.0 30 14.5 31 24.5 32 32.0 33 47.5 34 69.5 35 90.5 36 108.5 37 138.0 38 173.5 39 182.0 40 189.0 41 211.0 42 237.0 43 259.5 44 250.0 45 248.5 46 271.5 47 274.5 48 234.5 49 164.5 50 135.5 51 139.0 52 109.0 53 76.5 54 62.5 55 54.0 56 41.0 57 32.0 58 24.5 59 13.5 60 11.0 61 8.0 62 7.0 63 3.0 64 0.0 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.5 71 0.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.125 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.03 25-29 0.05 30-34 0.034999999999999996 35-39 0.03 40-44 0.04 45-49 0.034999999999999996 50-54 0.025 55-59 0.05 60-64 0.03 65-69 0.02 70-74 0.05 75-79 0.04 80-84 0.025 85-89 0.045 90-94 0.03 95-99 0.025 100-104 0.05 105-109 0.045 110-114 0.03 115-119 0.04 120-124 0.05 125-129 0.045 130-134 0.05 135-139 0.05 140-144 0.03 145-149 0.05 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.025 #Duplication Level Percentage of deduplicated Percentage of total 1 70.5179282868526 48.675000000000004 2 19.304599782687433 26.650000000000002 3 6.772908366533864 14.025000000000002 4 2.2817819630568636 6.3 5 0.7243752263672582 2.5 6 0.14487504527345166 0.6 7 0.21731256791017745 1.05 8 0.036218761318362915 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGCTTGAAGAAAGTGAGCTCATCAAGGTTCACAAACTCCAAAATTTCACC 8 0.2 No Hit TATTGGGAATGCTTCTGCAAATGTATCTTCAATATATACTCCATTTATTT 7 0.17500000000000002 No Hit GAATTAATGCAATCGGATTCTTCACAGTGTGCAGTTTGTAAGGATAGTTT 7 0.17500000000000002 No Hit CCTACACAGTCGGGTTGAACCGATTTGCTGATCTGACCAACGAGGAGTTC 7 0.17500000000000002 No Hit GCACTGATTTCTGCAGATCTAGATCTTGGAGAAATAGCTAGGGCAAAATT 7 0.17500000000000002 No Hit GTAGTTTTGTATGAAGATGAACTGGCTGATAATGGGGTTTCACTTTTAAC 7 0.17500000000000002 No Hit CTAGTGTTATGTCTGATTCACAATTCATGCTCCACCTCTGGTCATGGTTC 7 0.17500000000000002 No Hit ACCAATTTTCACTTCATCACTGAGATTTTCACTTCTGGGACATTGCGGCA 6 0.15 No Hit GATAAAGAAGGAGGTTGCTGAGATGATTACGACACGTATCCCTTCTGTGG 6 0.15 No Hit GTTGAGAGAAAAATGTTTTCTTCCTCAGTCTGCATGCCGATTTCACCATC 6 0.15 No Hit CTTGAAAAACTGAACGTTAGGCCAGATATCTATGTTGTATTGGATGTTCC 6 0.15 No Hit ATGCTCTTTGCCTTTGGCTTGGCTTGGCTTGTTACCCTTCCTATTGGGGT 5 0.125 No Hit ATTTATGCCATCATTGAACTAAAAGGGGAGAAAGAGAATTTCTCCACTCT 5 0.125 No Hit CAAAAGTGGTAATATTGCAGCTCCAAGGAATGGAGAATCTAATGAAATTT 5 0.125 No Hit TATGCATCTTGCTTGTGGTTTTCACGGCCAGATCTCGTGCTGCAAAGAGG 5 0.125 No Hit GGCAGAGATTATTGAGCATGGTGTATCAGGATTCCATATGGATCCGTATT 5 0.125 No Hit GTCATATTTCAGCATCAAACTTTCTGCTTCAGTTCCACCTTGCCTATGCC 5 0.125 No Hit CTCAATATTTCAGAATGCAATTCAGAAATGAAAGGAAGTCATTGTACTAT 5 0.125 No Hit AACAACTCCAAAACGATGTCGTCTGGAAGAAAGATATCCCTAAGGAGCAG 5 0.125 No Hit CTGGTGAATATGGTGGATGGAAGCTGGAGAAAACTGTTGATTATTTTATG 5 0.125 No Hit TGGTAGTGGTGCAGGTGGCGGCGTTGGTGGTCTCGTAACTAAAAAATGGC 5 0.125 No Hit TGCAAGAATTAACTCTGACATGAAACTATGGTCTGATGACGACAAACCTA 5 0.125 No Hit CAAAATGCGAATATTCATTTGCCTGTTCCGGATATGCTGAAATCTTCCAA 5 0.125 No Hit CTTCAGTTCCAAAATCTAAACGAGGAGAAGAGAAACATACATTTGGAGGT 5 0.125 No Hit ATTTGGAGTTATGTCAGATTGGGAGCAACATGTTATTGATCGTGGGGACC 5 0.125 No Hit GAGGAGTTGAAGGTGAAAGAAATTAAGAATGGGCGCCTAGCAATGGTTGC 5 0.125 No Hit GATGATGGGAAAGGTAGGAAGTTGGAGAATGGACACTCCAATGGGAACCA 5 0.125 No Hit GATTGCTAGTGATGCTATTCTGAGGCTAGCTGGGGTTGTGGAGCATGGAA 5 0.125 No Hit GGAACCAATACATGGGACAGATTTGAGCTTCGTGTCCACAAGCGTGTAAT 5 0.125 No Hit GATTGTGCCTTAAAGACACTCAAGTCAGGAGGACCGTTCAAATTTTACAC 5 0.125 No Hit CTTCTTTCTTTTTCTTTTTGCCTGGTTGCACTTTATTGGGAAAAAAAGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.36250000000000004 0.0 0.0 0.0 0.0 86-87 0.4375 0.0 0.0 0.0 0.0 88-89 0.55 0.0 0.0 0.0 0.0 90-91 0.65 0.0 0.0 0.0 0.0 92-93 0.7875000000000001 0.0 0.0 0.0 0.0 94-95 0.95 0.0 0.0 0.0 0.0 96-97 1.15 0.0 0.0 0.0 0.0 98-99 1.35 0.0 0.0 0.0 0.0 100-101 1.475 0.0 0.0 0.0 0.0 102-103 1.6625 0.0 0.0 0.0 0.0 104-105 1.8875 0.0 0.0 0.0 0.0 106-107 1.9249999999999998 0.0 0.0 0.0 0.0 108-109 2.2 0.0 0.0 0.0 0.0 110-111 2.5250000000000004 0.0 0.0 0.0 0.0 112-113 2.675 0.0 0.0 0.0 0.0 114-115 2.975 0.0 0.0 0.0 0.0 116-117 3.3375 0.0 0.0 0.0 0.0 118-119 3.675 0.0 0.0 0.0 0.0 120-121 4.225 0.0 0.0 0.0 0.0 122-123 4.4625 0.0 0.0 0.0 0.0 124-125 5.0 0.0 0.0 0.0 0.0 126-127 5.525 0.0 0.0 0.0 0.0 128-129 6.2875 0.0 0.0 0.0 0.0 130-131 6.975 0.0 0.0 0.0 0.0 132-133 7.5375 0.0 0.0 0.0 0.0 134-135 8.3375 0.0 0.0 0.0 0.0 136-137 9.0625 0.0 0.0 0.0 0.0 138-139 9.6 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTGT 10 0.006830828 145.0 5 ACGTTTG 10 0.006830828 145.0 4 ACTGAAG 10 0.006830828 145.0 145 CACGTTT 10 0.006830828 145.0 3 TGTGGGA 10 0.006830828 145.0 9 TTGTGGG 10 0.006830828 145.0 8 TTTGTGG 10 0.006830828 145.0 7 GTGTAGG 40 0.005621335 54.375 145 >>END_MODULE Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004577 spots for SRR13695485.sra Written 1004577 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra Read 1004567 spots for SRR13695485.sra Written 1004567 spots for SRR13695485.sra SRR ids: ['SRR13695485.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_jyw_xcsc SRR13695485.sra spots: 20091350 blocks: [[1, 1004567], [1004568, 2009134], [2009135, 3013701], [3013702, 4018268], [4018269, 5022835], [5022836, 6027402], [6027403, 7031969], [7031970, 8036536], [8036537, 9041103], [9041104, 10045670], [10045671, 11050237], [11050238, 12054804], [12054805, 13059371], [13059372, 14063938], [14063939, 15068505], [15068506, 16073072], [16073073, 17077639], [17077640, 18082206], [18082207, 19086773], [19086774, 20091350]] SRR13695485 file size 6806219 SRR13695485 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695485 SRR13695485_1.fastq SRR13695485_2.fastq Input file: SRR13695485_1.fastq Paired file: SRR13695485_2.fastq trimmed: SRR13695485-trimmed-pair1.fastq, SRR13695485-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 06:43:30 2025 >> started Wed Feb 12 06:43:54 2025 >> done (23.460s) 20091350 read pairs processed; of these: 108 ( 0.00%) short read pairs filtered out after trimming by size control 1977 ( 0.01%) empty read pairs filtered out after trimming by size control 20089265 (99.99%) read pairs available; of these: 2479687 (12.34%) trimmed read pairs available after processing 17609578 (87.66%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 2 0.00% 20 6 0.00% 21 0 0.00% 22 5 0.00% 23 3 0.00% 24 2 0.00% 25 5 0.00% 26 5 0.00% 27 4 0.00% 28 4 0.00% 29 0 0.00% 30 7 0.00% 31 4 0.00% 32 4 0.00% 33 9 0.00% 34 3 0.00% 35 9 0.00% 36 7 0.00% 37 6 0.00% 38 9 0.00% 39 8 0.00% 40 15 0.00% 41 14 0.00% 42 13 0.00% 43 16 0.00% 44 27 0.00% 45 31 0.00% 46 34 0.00% 47 47 0.00% 48 44 0.00% 49 88 0.00% 50 75 0.00% 51 104 0.00% 52 104 0.00% 53 106 0.00% 54 124 0.00% 55 151 0.00% 56 225 0.00% 57 222 0.00% 58 230 0.00% 59 299 0.00% 60 352 0.00% 61 417 0.00% 62 500 0.00% 63 502 0.00% 64 622 0.00% 65 724 0.00% 66 780 0.00% 67 911 0.00% 68 1042 0.01% 69 1207 0.01% 70 1456 0.01% 71 1615 0.01% 72 1765 0.01% 73 2015 0.01% 74 2328 0.01% 75 2658 0.01% 76 2891 0.01% 77 3292 0.02% 78 3593 0.02% 79 4233 0.02% 80 4583 0.02% 81 4950 0.02% 82 5498 0.03% 83 6117 0.03% 84 6766 0.03% 85 7343 0.04% 86 7800 0.04% 87 8461 0.04% 88 9414 0.05% 89 9628 0.05% 90 10797 0.05% 91 11422 0.06% 92 11847 0.06% 93 13003 0.06% 94 13854 0.07% 95 14700 0.07% 96 15346 0.08% 97 16305 0.08% 98 16764 0.08% 99 17955 0.09% 100 18594 0.09% 101 19441 0.10% 102 20517 0.10% 103 21252 0.11% 104 22546 0.11% 105 23486 0.12% 106 24299 0.12% 107 25841 0.13% 108 26023 0.13% 109 27272 0.14% 110 28041 0.14% 111 29332 0.15% 112 30035 0.15% 113 30813 0.15% 114 32257 0.16% 115 33705 0.17% 116 34454 0.17% 117 35844 0.18% 118 36334 0.18% 119 37163 0.18% 120 38278 0.19% 121 39453 0.20% 122 40438 0.20% 123 41723 0.21% 124 43220 0.22% 125 43711 0.22% 126 45673 0.23% 127 46451 0.23% 128 47297 0.24% 129 47926 0.24% 130 49320 0.25% 131 49875 0.25% 132 50935 0.25% 133 52275 0.26% 134 53204 0.26% 135 54569 0.27% 136 55538 0.28% 137 56074 0.28% 138 57417 0.29% 139 58759 0.29% 140 59687 0.30% 141 59819 0.30% 142 61208 0.30% 143 62242 0.31% 144 63171 0.31% 145 64407 0.32% 146 64183 0.32% 147 65702 0.33% 148 66647 0.33% 149 67608 0.34% 150 68095 0.34% 151 17609578 87.66% 20089265 reads passed initial QC criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=2.48 fanout-score-rank=18 prefix-density=0.38 prefix-fanout=2.3 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.10 sequence-density-rank=24 fanout-score=28.93 fanout-score-rank=1 prefix-density=0.32 prefix-fanout=8.6 sequence=CCATCTTCTTCATCTATAGATTTCAATCACAACAG criterion=sequence-density sequence-density=0.57 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=29 prefix-density=0.57 prefix-fanout=2.0 sequence=AACCGCACCCCGGCACA criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=18.77 fanout-score-rank=1 prefix-density=0.06 prefix-fanout=2.0 sequence=GCCAAAACTCTGCATTTGCTAGCTAGAAGTCACTCTACCCTTCGCATTACTTCCTCAATCAACCACTGCTGTTTGATCATGGCAGCAACCATCTCAACCGTTGGAGCTGTCAACACAGCACCGCTGGCTTTGAATGGCTCTGGCGCTGGATCCACAGTCCCAA SRR13695485 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 06:44:36 Started mapping on | Feb 12 06:44:36 Finished on | Feb 12 06:46:52 Mapping speed, Million of reads per hour | 531.77 Number of input reads | 20089265 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 18671419 Uniquely mapped reads % | 92.94% Average mapped length | 294.30 Number of splices: Total | 18265965 Number of splices: Annotated (sjdb) | 17874363 Number of splices: GT/AG | 17896126 Number of splices: GC/AG | 303667 Number of splices: AT/AC | 10602 Number of splices: Non-canonical | 55570 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.88 Insertion rate per base | 0.02% Insertion average length | 2.44 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 449865 % of reads mapped to multiple loci | 2.24% Number of reads mapped to too many loci | 107635 % of reads mapped to too many loci | 0.54% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.15% % of reads unmapped: other | 0.14% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 968200 968200 968200 N_multimapping 449865 449865 449865 N_noFeature 830969 18320323 1047999 N_ambiguous 245906 1577 110707 UnstrandedReadsAssigned:17594544 PositiveStrandReadsAssigned:349519 NegativeStrandReadsAssigned:17512713 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695485 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695485-trimmed-pair1.fastq SRR13695485-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,089,265 reads, 17,679,103 reads pseudoaligned [quant] estimated average fragment length: 244.995 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,026 rounds 52401 SRR13695485.ke.tsv 34699 SRR13695485.se.tsv 87100 total ==> SRR13695485.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1774.01 628 21.3426 Potri.005G024800.1.v4.1 1035 791.005 304 23.1706 Potri.004G059700.1.v4.1 961 717.077 0 0 Potri.007G009000.2.v4.1 1416 1172.01 0 0 Potri.003G141000.2.v4.1 2943 2699.01 1064.49 23.7783 Potri.016G087400.1.v4.1 270 87.0361 553.087 383.121 Potri.015G069301.1.v4.1 564 328.985 0 0 Potri.010G195200.1.v4.1 1773 1529.01 62 2.4447 Potri.012G127500.1.v4.1 977 733.036 68 5.59276 ==> SRR13695485.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 259 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 288 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 4 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 22 SRR13695485 completed mapping pipeline successfully