Starting /dee2/code/volunteer_pipeline.sh SRR13695486 current disk space = 3050080874496 free memory = 1582643628 SRR13695486 SRAfilesize f7b5494d37d280cabd63aff87b4cbc0d SRR13695486.sra SRR13695486.sra file validated SRR13695486 is paired end SRR13695486 is conventional basespace SRR13695486 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695486_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.524 37.0 37.0 37.0 37.0 37.0 2 36.2475 37.0 37.0 37.0 37.0 37.0 3 36.577 37.0 37.0 37.0 37.0 37.0 4 36.617 37.0 37.0 37.0 37.0 37.0 5 36.5365 37.0 37.0 37.0 37.0 37.0 6 36.6295 37.0 37.0 37.0 37.0 37.0 7 36.4465 37.0 37.0 37.0 37.0 37.0 8 36.537 37.0 37.0 37.0 37.0 37.0 9 36.587 37.0 37.0 37.0 37.0 37.0 10-14 36.560199999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.561400000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.525 37.0 37.0 37.0 37.0 37.0 25-29 36.514300000000006 37.0 37.0 37.0 37.0 37.0 30-34 36.4336 37.0 37.0 37.0 37.0 37.0 35-39 36.4418 37.0 37.0 37.0 37.0 37.0 40-44 36.394400000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.3448 37.0 37.0 37.0 37.0 37.0 50-54 36.3525 37.0 37.0 37.0 37.0 37.0 55-59 36.3928 37.0 37.0 37.0 37.0 37.0 60-64 36.359500000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.3015 37.0 37.0 37.0 37.0 37.0 70-74 36.3346 37.0 37.0 37.0 37.0 37.0 75-79 36.280899999999995 37.0 37.0 37.0 37.0 37.0 80-84 36.208600000000004 37.0 37.0 37.0 37.0 37.0 85-89 36.2456 37.0 37.0 37.0 37.0 37.0 90-94 36.130700000000004 37.0 37.0 37.0 37.0 37.0 95-99 36.1329 37.0 37.0 37.0 37.0 37.0 100-104 36.1619 37.0 37.0 37.0 37.0 37.0 105-109 36.1632 37.0 37.0 37.0 37.0 37.0 110-114 36.1 37.0 37.0 37.0 37.0 37.0 115-119 36.1191 37.0 37.0 37.0 37.0 37.0 120-124 36.0152 37.0 37.0 37.0 37.0 37.0 125-129 36.0396 37.0 37.0 37.0 37.0 37.0 130-134 36.03009999999999 37.0 37.0 37.0 37.0 37.0 135-139 35.9144 37.0 37.0 37.0 37.0 37.0 140-144 35.8603 37.0 37.0 37.0 37.0 37.0 145-149 35.741 37.0 37.0 37.0 37.0 37.0 150-151 35.518 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 2.0 24 3.0 25 0.0 26 1.0 27 5.0 28 16.0 29 21.0 30 24.0 31 43.0 32 42.0 33 66.0 34 132.0 35 326.0 36 2956.0 37 362.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.25 10.8 8.774999999999999 41.175 2 18.89111891620672 15.102860010035124 36.75363773206222 29.252383341695936 3 19.35 16.05 27.325 37.275000000000006 4 24.025 24.275 22.875 28.825 5 23.625 31.35 25.05 19.975 6 20.75 33.35 23.849999999999998 22.05 7 14.625 26.85 42.175000000000004 16.35 8 18.925 25.724999999999998 31.825 23.525 9 18.725 23.225 34.949999999999996 23.1 10-14 19.445 29.630000000000003 27.334999999999997 23.59 15-19 18.44 28.410000000000004 29.28 23.87 20-24 19.875 28.015 28.294999999999998 23.815 25-29 19.715 28.23 27.515 24.54 30-34 19.34 28.310000000000002 28.305000000000003 24.044999999999998 35-39 19.865 28.585 27.894999999999996 23.655 40-44 19.585 27.91 28.610000000000003 23.895 45-49 19.71 28.925 27.825 23.54 50-54 19.64 28.57 28.410000000000004 23.380000000000003 55-59 19.855 28.24 27.51 24.395 60-64 19.75 28.08 27.905 24.265 65-69 20.54 28.585 27.584999999999997 23.29 70-74 20.79 28.000000000000004 27.560000000000002 23.65 75-79 20.79 28.595 26.889999999999997 23.724999999999998 80-84 19.395 28.365000000000002 28.38 23.86 85-89 20.06 28.46 27.485 23.995 90-94 20.19 27.425 28.084999999999997 24.3 95-99 20.01 28.050000000000004 27.794999999999998 24.145 100-104 20.025000000000002 28.910000000000004 27.779999999999998 23.285 105-109 20.29 28.395 27.175 24.14 110-114 20.335 27.915 27.605 24.145 115-119 19.900000000000002 28.425 27.474999999999998 24.2 120-124 20.89 28.305000000000003 27.034999999999997 23.77 125-129 20.89 28.360000000000003 27.169999999999998 23.580000000000002 130-134 20.57 29.28 26.634999999999998 23.515 135-139 21.349999999999998 28.23 26.93 23.49 140-144 21.235 28.4 26.875 23.49 145-149 20.865000000000002 28.24 26.96 23.935000000000002 150-151 19.75 28.6875 27.537499999999998 24.025 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 2.0 16 2.0 17 0.0 18 0.0 19 0.0 20 0.0 21 2.0 22 2.0 23 2.0 24 4.0 25 3.0 26 4.5 27 7.5 28 10.0 29 16.0 30 21.5 31 30.5 32 35.5 33 38.5 34 57.0 35 70.0 36 86.0 37 107.5 38 129.5 39 154.0 40 174.5 41 208.0 42 224.0 43 260.0 44 272.0 45 264.5 46 261.5 47 240.0 48 233.5 49 216.5 50 185.0 51 137.0 52 123.0 53 100.0 54 70.5 55 61.0 56 45.5 57 43.0 58 30.5 59 18.5 60 17.0 61 9.5 62 3.0 63 1.0 64 1.0 65 1.5 66 2.0 67 2.0 68 1.0 69 1.5 70 1.0 71 0.5 72 2.5 73 2.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.35000000000000003 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.85 #Duplication Level Percentage of deduplicated Percentage of total 1 69.44652206432312 46.425 2 19.07255048616305 25.5 3 6.881077038145101 13.8 4 2.804786836200449 7.5 5 1.1219147344801794 3.75 6 0.3365744203440539 1.35 7 0.2617801047120419 1.225 8 0.037397157816005985 0.2 9 0.0 0.0 >10 0.037397157816005985 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTCGAGGGAGTACTTGTAAGGCTTGCCTGACAGCTTCCATAGATCCCTG 10 0.25 No Hit GTAGAAATCTGGTTTGATGGAGTTGCTTGGACTTTGAAGCATCGACAATG 8 0.2 No Hit CCTCCTCCCACCGCTGCTCTCTTCTATGTTCTTCATCTCCACGTTGACTC 7 0.17500000000000002 No Hit GGCGTTGTTGTTTACTGGGTCAGAAAGGTGGTCAGCCAGGTTCTCCAGTG 7 0.17500000000000002 No Hit GGGAGTTTCGACAAGGTAACAGGAGCTGCTTGTGCCATTCTTGGATAAGT 7 0.17500000000000002 No Hit ATCTATTCTGAGGGGGTATAACCCGTGATCTCGTTGCATATGATCCACCC 7 0.17500000000000002 No Hit CTGGGTCAGAAAGGTGGTCAGCAAGGTTCTCCAGTGGTCCCTTTCCGGTC 7 0.17500000000000002 No Hit CTTGTTTAACCTTAATTTAAAGTCCTGCCACTTGCTTTTTTTGGATCCAT 7 0.17500000000000002 No Hit GAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTTGGAGGCCACACC 7 0.17500000000000002 No Hit CTCGTGACTACATGAATGATGCAAGCGAACACATGGTGATTACATGAAAT 6 0.15 No Hit CTCTGCTTTTACTATTGGTTTTGCCATACGCCTCAAGCCCACCTTTGTTC 6 0.15 No Hit GTCCACCCGTGCCAGTAATCATGGTCAATTGTGAGCAACTTGGTAATAGC 6 0.15 No Hit GTCCACAGTTCTACAGAATAAGGCCATAAATCCAGAACCTCCAAGACCTT 6 0.15 No Hit GTTTGTTAACCTCTTTTAGCCCCTTCCTGAAGAAAATCATGGCCCCACGT 6 0.15 No Hit CCGGGATCTATAATTATTGGCTTCGCCCTTTGAAACTCTTTCCATCCAAT 6 0.15 No Hit ACCAACTGCAGCATTACAAGATGGATCAGGACATCGCAACATCAAACATC 6 0.15 No Hit GCCGTTCTCAGCTTCTGCCATTGGCGTGGAATTGATGCTAACATCAGGAA 6 0.15 No Hit GGGAAAATCACCACATCCCTTTCTTCCTTGCCCTCCACTGGATTGGTAGG 6 0.15 No Hit CAGGAGAGTCACTGACTTTGAACATGCCAAGCATCCTTTTAGCATCAACT 5 0.125 No Hit GTTCCTCATGTTGTAGGAATAGTTTTCCAAAGCATTCTTTGCCTCAACCT 5 0.125 No Hit TCCTGAACAACGTTATAGCCTTCACTTGTCTCGCCATAATCCTTCACAAC 5 0.125 No Hit CTACACTTCAACTGATCCATTTAAGGATTCGTCTAAACTTTACAGATGAC 5 0.125 No Hit CTTGTGCTTGATCTTCTTGGGCTTGGTGTAGGTCTTCTTCTTTCTCTTCT 5 0.125 No Hit CTTTACGGTACATATTCTAAACACTAAAACATGTGCTTAACACTAAATAT 5 0.125 No Hit GCTGGCTCTGGAGCTTGACCTTGATGGTGTCAAAGGGGTGCCCAACTAAC 5 0.125 No Hit GTCAAGAGTGTAGGTTAAACGTGTCATAAGCTTAGTTTTTTGGAAATCAG 5 0.125 No Hit CCTTCATTCACTGAATAAATCTTTCCTTTCTTTGGAATCTTTATGTCAGG 5 0.125 No Hit GGGACATACTTGCTAGAAACCTCAAACAAGTTACCATAAACATTATACTG 5 0.125 No Hit CCCGTAATTGCCCCTTCCTTTTGGCTGGTTACAAGCACAACAATTCCCTT 5 0.125 No Hit TAAGGCCACTGGATTTCCCAATACGGCTTATGGTTGCCAGATATATAGCC 5 0.125 No Hit ATCCCATACCAAACCTTTTGATGGAGGAGCAAATAAAAGAATTCAAAGCA 5 0.125 No Hit CACAGCGTTTCAACTCCTTTTTTCTCTAGTTGCATTTTCAATTTAAATTG 5 0.125 No Hit GGGGAGTTGAAGGTTCAGACAAACTGCTGGTCGAGTCACCTGTTGTTGGA 5 0.125 No Hit GGTTTATGAAGGAATCTATCACAACAAAAGAAGAAATCTCTCTGTTCCTC 5 0.125 No Hit GCTTCAGCTACACTGCTAAACAAAGATTGCTTGCTCTTCTTGGGCTCTTC 5 0.125 No Hit GGAGATTTCGGAGCTCTGGTACTGCTTGAACTCAGACTTGGTGATTTTCC 5 0.125 No Hit GTCGGGGGCTTTCTCCTTCATTTTCTCAAATTCCTCCTTGTAGAGCAATG 5 0.125 No Hit CTGTTTTTGGCAAGGGATATTGACAGTACTCTGATGAAGCAGAACTATCA 5 0.125 No Hit CGTTACAGGAGGCATCTGAAGTATGTTTTGTGCACCAGGAATTTCATAAA 5 0.125 No Hit GTTTGGTATATCACTTCTTTCTGCCTTCTCCACAATCACCGGAATCCTAT 5 0.125 No Hit CATAAAACTGCAACTCTTTTGCTGCAGAATTTCCTGTAACCTACCATTTT 5 0.125 No Hit GTTGTTGGGTCATCAACGAAGCGGCCACGATCACCCAAAGCTCCAATGGC 5 0.125 No Hit CTCTGCAACAGTTAACCCAGCAGCAGCTGCTGCCTCCAAATCTATATGAT 5 0.125 No Hit CGTGGCTGCAGAATTAAGTCCTTTCAACCCACTGCAGCAAGCTGCAGGCA 5 0.125 No Hit CCCCATTCCCATATTTTTTGGCCTCATCTTTGCTTCAATTGGGGCTACAA 5 0.125 No Hit GCTTATTTTTCTCACATAGATAGTTCTGATCATTTTACATATTGATAAAG 5 0.125 No Hit CCAGACAGTACATATAGTAACTACAATCATACTTGGCCAGCTCATCCAAT 5 0.125 No Hit TTTCATTGAACCGAAGAATAAACATCCCCAGCAAGTGAACATAAGGTAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0125 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.0875 0.0 0.0 0.0 0.0 88-89 0.1125 0.0 0.0 0.0 0.0 90-91 0.25 0.0 0.0 0.0 0.0 92-93 0.275 0.0 0.0 0.0 0.0 94-95 0.3125 0.0 0.0 0.0 0.0 96-97 0.375 0.0 0.0 0.0 0.0 98-99 0.3875 0.0 0.0 0.0 0.0 100-101 0.44999999999999996 0.0 0.0 0.0 0.0 102-103 0.475 0.0 0.0 0.0 0.0 104-105 0.6125 0.0 0.0 0.0 0.0 106-107 0.9375 0.0 0.0 0.0 0.0 108-109 1.25 0.0 0.0 0.0 0.0 110-111 1.6 0.0 0.0 0.0 0.0 112-113 1.775 0.0 0.0 0.0 0.0 114-115 1.9875 0.0 0.0 0.0 0.0 116-117 2.2625 0.0 0.0 0.0 0.0 118-119 2.375 0.0 0.0 0.0 0.0 120-121 2.625 0.0 0.0 0.0 0.0 122-123 2.9375 0.0 0.0 0.0 0.0 124-125 3.3875 0.0 0.0 0.0 0.0 126-127 3.7625 0.0 0.0 0.0 0.0 128-129 4.35 0.0 0.0 0.0 0.0 130-131 4.9 0.0 0.0 0.0 0.0 132-133 5.3875 0.0 0.0 0.0 0.0 134-135 6.0375 0.0 0.0 0.0 0.0 136-137 6.3125 0.0 0.0 0.0 0.0 138-139 6.9875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATGCA 10 0.006830828 145.0 145 GTCACTT 10 0.006830828 145.0 1 CCTACAT 10 0.006830828 145.0 1 CAATAGG 10 0.006830828 145.0 8 CAGGACA 10 0.006830828 145.0 3 ACAATAG 10 0.006830828 145.0 7 CCAGGAC 10 0.006830828 145.0 2 >>END_MODULE SRR13695486 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695486_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.10575 37.0 37.0 37.0 37.0 37.0 2 35.9875 37.0 37.0 37.0 37.0 37.0 3 36.143 37.0 37.0 37.0 37.0 37.0 4 36.0125 37.0 37.0 37.0 37.0 37.0 5 36.068 37.0 37.0 37.0 37.0 37.0 6 36.1285 37.0 37.0 37.0 37.0 37.0 7 36.2295 37.0 37.0 37.0 37.0 37.0 8 36.1555 37.0 37.0 37.0 37.0 37.0 9 36.1385 37.0 37.0 37.0 37.0 37.0 10-14 36.1736 37.0 37.0 37.0 37.0 37.0 15-19 36.174 37.0 37.0 37.0 37.0 37.0 20-24 36.10715 37.0 37.0 37.0 37.0 37.0 25-29 36.03785 37.0 37.0 37.0 37.0 37.0 30-34 36.028499999999994 37.0 37.0 37.0 37.0 37.0 35-39 36.07845 37.0 37.0 37.0 37.0 37.0 40-44 36.043899999999994 37.0 37.0 37.0 37.0 37.0 45-49 36.01095 37.0 37.0 37.0 37.0 37.0 50-54 35.887899999999995 37.0 37.0 37.0 37.0 37.0 55-59 35.884249999999994 37.0 37.0 37.0 37.0 37.0 60-64 35.94045 37.0 37.0 37.0 37.0 37.0 65-69 35.9193 37.0 37.0 37.0 37.0 37.0 70-74 35.82895 37.0 37.0 37.0 37.0 37.0 75-79 35.82005 37.0 37.0 37.0 37.0 37.0 80-84 35.863150000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.681149999999995 37.0 37.0 37.0 37.0 37.0 90-94 35.74575 37.0 37.0 37.0 37.0 37.0 95-99 35.6816 37.0 37.0 37.0 37.0 37.0 100-104 35.66415 37.0 37.0 37.0 37.0 37.0 105-109 35.6668 37.0 37.0 37.0 37.0 37.0 110-114 35.59495 37.0 37.0 37.0 37.0 37.0 115-119 35.66695000000001 37.0 37.0 37.0 37.0 37.0 120-124 35.46274999999999 37.0 37.0 37.0 37.0 37.0 125-129 35.573699999999995 37.0 37.0 37.0 37.0 37.0 130-134 35.4702 37.0 37.0 37.0 37.0 37.0 135-139 35.401250000000005 37.0 37.0 37.0 37.0 37.0 140-144 35.35435 37.0 37.0 37.0 32.2 37.0 145-149 35.22205 37.0 37.0 37.0 29.8 37.0 150-151 34.916125 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 3.0 14 1.0 15 0.0 16 2.0 17 1.0 18 2.0 19 0.0 20 0.0 21 3.0 22 4.0 23 4.0 24 7.0 25 8.0 26 9.0 27 15.0 28 7.0 29 19.0 30 27.0 31 54.0 32 66.0 33 118.0 34 220.0 35 674.0 36 2597.0 37 158.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.92325056433409 22.54828191622774 11.78831201404565 27.74015550539253 2 27.0 25.900000000000002 30.75 16.35 3 22.35 28.175 29.4 20.075000000000003 4 23.200000000000003 36.425000000000004 22.925 17.45 5 26.375 36.05 22.075 15.5 6 21.55 40.025 22.2 16.225 7 22.5 22.45 36.95 18.099999999999998 8 22.925 24.85 27.375 24.85 9 22.925 25.25 29.25 22.575 10-14 23.505000000000003 29.895 25.955000000000002 20.645 15-19 24.0 28.78 26.705000000000002 20.515 20-24 23.76569456255315 27.667450352658697 27.77749987494372 20.78935520984443 25-29 23.47434292866083 27.989987484355446 27.759699624530665 20.77596996245307 30-34 23.4714300010007 27.984589212448714 27.734414089862902 20.809566696687682 35-39 22.430093542093942 27.627432344555046 28.207693462057925 21.734780651293082 40-44 24.452116481537075 27.469228459921947 27.789452616831785 20.289202441709197 45-49 22.487368052428835 27.635199359647807 28.43563960178098 21.441792986142378 50-54 23.22696809042713 28.50855256576973 27.793338001400418 20.47114134240272 55-59 23.25907384230288 28.390488110137674 27.429286608260327 20.921151439299123 60-64 23.88574858686409 28.007603421539695 27.822520134060326 20.284127857535893 65-69 23.109621924384875 28.380676135227045 27.170434086817362 21.339267853570714 70-74 23.204005006257823 27.934918648310386 27.704630788485606 21.15644555694618 75-79 22.96952409548116 28.038833008056844 27.593454436270832 21.39818846019116 80-84 23.75093773443361 27.94698674668667 27.09677419354839 21.205301325331334 85-89 24.015215976775615 27.143500675709497 28.164572801441512 20.676710546073377 90-94 23.905757590915915 27.507378320244108 27.897553899254664 20.689310189585314 95-99 23.001900570171053 28.74362308692608 27.54326297889367 20.711213364009204 100-104 22.508135168961203 28.660826032540676 27.37922403003755 21.451814768460576 105-109 23.59595555110622 28.28110922014216 27.700470517569325 20.4224647111823 110-114 24.110849882447102 28.80796358361263 27.20724325946676 19.87394327447351 115-119 24.543316150342825 28.437015164406187 27.34097392522897 19.67869476002202 120-124 23.98498122653317 28.67083854818523 27.229036295369212 20.115143929912392 125-129 24.69222300070063 28.13532178961065 26.894204784305874 20.278250425382844 130-134 24.687093221187546 28.051466906979073 26.449384199459296 20.812055672374086 135-139 24.851063829787236 27.49436795994994 28.20025031289111 19.454317897371716 140-144 25.016257315792107 27.837526887099195 27.35230853884248 19.79390725826622 145-149 25.416770963704632 27.9549436795995 26.74843554443054 19.87984981226533 150-151 26.749280260357995 28.58931030166479 26.286143447239958 18.37526599073726 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 1.0 9 0.5 10 0.0 11 1.0 12 1.5 13 1.5 14 1.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 2.0 23 2.0 24 3.5 25 5.5 26 5.0 27 8.0 28 10.5 29 11.5 30 13.5 31 17.0 32 27.0 33 41.0 34 50.0 35 70.5 36 84.0 37 98.5 38 125.0 39 143.0 40 177.5 41 213.0 42 244.5 43 265.5 44 301.0 45 293.0 46 243.5 47 230.0 48 233.0 49 223.5 50 170.0 51 129.5 52 114.5 53 97.0 54 81.5 55 59.5 56 48.0 57 41.0 58 26.5 59 24.0 60 18.5 61 11.5 62 10.0 63 5.0 64 2.5 65 2.0 66 0.5 67 0.5 68 1.0 69 0.5 70 0.5 71 0.5 72 0.0 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.325 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.045 25-29 0.125 30-34 0.06999999999999999 35-39 0.045 40-44 0.06999999999999999 45-49 0.055 50-54 0.03 55-59 0.125 60-64 0.045 65-69 0.02 70-74 0.125 75-79 0.08499999999999999 80-84 0.025 85-89 0.105 90-94 0.045 95-99 0.03 100-104 0.125 105-109 0.11 110-114 0.045 115-119 0.095 120-124 0.125 125-129 0.09 130-134 0.13 135-139 0.125 140-144 0.045 145-149 0.125 150-151 0.13749999999999998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.55 #Duplication Level Percentage of deduplicated Percentage of total 1 70.68837897853442 47.75 2 18.46780162842339 24.95 3 6.18060695780903 12.525 4 2.775721687638786 7.5 5 1.2213175425610658 4.125 6 0.2590673575129534 1.05 7 0.2960769800148038 1.4000000000000001 8 0.03700962250185048 0.2 9 0.0 0.0 >10 0.07401924500370095 0.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCCTCTGTGATT 10 0.25 No Hit GTGGAAAACGGGTTGATCAAGTGGGACCGTCTATACCTGTACAGGTTATT 10 0.25 No Hit CTCTTCATTGCTACAAATCGTGATGCAGTCACCCATCTTACAGATGCTCA 8 0.2 No Hit CTCGGTGAGGTAACTGACCCAATCTACCCAGGTGGAAGCTTCGACCCACT 7 0.17500000000000002 No Hit GGGCTGTTGGTTTATTGGAGGAACCGGTTGTTCCATTTCCGGATGGTAGA 7 0.17500000000000002 No Hit GTTGAGGGTTACAGAATTGCTGGCGGGCCACTCGGTGAGGTAACTGACCC 7 0.17500000000000002 No Hit CTTGGACTTCCCTCGAGACCTGGGCAAGCCGTGTGTCCAGACTACAGTAT 7 0.17500000000000002 No Hit CAGAGAGGAAGCAAGAAATTTGCAGAGCAGAGATGGCATCCATAACCATG 7 0.17500000000000002 No Hit CGCTAGGGTTTTGTCAGGTTTCCATCCCTTTCTCTCCGGTGTTCTTCAAC 7 0.17500000000000002 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 7 0.17500000000000002 No Hit AACCGGCTACTCACCGGGGGGACATTGCAAGGGGAATCGAGGCTGGCGGT 7 0.17500000000000002 No Hit GGAGATTGGGATTGGTTTATTAATCTTAGTGCATCTGATTATCCACTTGT 6 0.15 No Hit AGTTTGTTCTGCTGAGGAAGTTGATCAAACACAAAAGGAACTAGATCGTG 6 0.15 No Hit GAGTTGAAGCCTGGTGGTCGAATTCCTGAAAAAGGAAAGACAATCACTTT 6 0.15 No Hit TGTAATACAGTAGAGAAACTTAAACCTGACACTTTGGTCATGGGAAGCCA 6 0.15 No Hit GTTGAACCAGTCTAGGCTCGGTCCTCAAAGCCAAAAGGGTCAACAACGTA 6 0.15 No Hit AGAACTGATAATCACAAATACCGATAACAAGCCATTTTCATTCACTAGTG 6 0.15 No Hit GACAATGCCATACAGATTGAATGAGAGCACTGGCTATATTGACTATGATC 6 0.15 No Hit AGAAAAATGGGACTAAGAACTCTTCCCTTGTCACCTTCACGAAACTGTTT 5 0.125 No Hit GTTAGCCGCGGCCAAGTCTCTGGATACCCAATTTACTGCTCTGCGCCAGG 5 0.125 No Hit CGTGAAAGGAAATGAGGATGCTGTGGAAGTCATTAAGAATGCCTTGTCAA 5 0.125 No Hit CAATGTCAAGGCCAAGATTCAAGACAAGGAGGGCATCCCACCAGACCAAC 5 0.125 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit CCGGATCCTCAGCACAGGAAGCTGAATTACAAAGTTCATGGCTGGCAAGT 5 0.125 No Hit GTAGTAGTTGAGGCCTTTTTTAGTGTAGAATCCACATTCCTCAGTAACCA 5 0.125 No Hit AAGTCCCTTGCTCATGGTGGACTTGCCCGGGGCCTCCATGAAGGTGCCAA 5 0.125 No Hit GGGTAGGCTGTCCAAGGAAGACATCGAGAAGATGGTTCAAGAGGCTGAGA 5 0.125 No Hit CTGCTATTAATACCACTAAAGAGTCTAGCAGCTCTGGTTCAACATCAGAG 5 0.125 No Hit TGCACCGCCAATTTTTGGAAGTGTTGGCTTGATAGTTCTTGGTGCATTTA 5 0.125 No Hit CAGCAAGTGGGCAATAACACCTTTGTTTCTTCCCATGATTTAAGTCATCT 5 0.125 No Hit AAATTTAAATTGTGCATTGCAAAAGCTGTATTAGCAATAAGGGTATTTTC 5 0.125 No Hit AAGCTGGCCTGTGCCATGCTTGTAGCGATGGTTGTTAGTGCACCACTAGC 5 0.125 No Hit GAATTGGCCGAGCGAGACGTATCCCGGGGAGCTCTCAACCCCCATAAACC 5 0.125 No Hit ATTAGGGAGGGGTTAATTAGCTATTAATTACTATGAAGAAGAATATGTAA 5 0.125 No Hit GTTCCATCCTAGTAGTGAAAATTTTGATAGTGGAGTGGATGATCTTGAAA 5 0.125 No Hit CTCAAAATTAGCAAAGGAGACATCACCAAGTGGTCTGTTGATGGCTCCTC 5 0.125 No Hit GTGTTTTACAAAGCAAATGAGTATGCTTCATTGAATCCTAATTTTGTGGG 5 0.125 No Hit TTTGAATCCTGTAAGGCCAGCCAACATGGGGTTTACGCCACCGTGCCTCT 5 0.125 No Hit CATCATCACAACCATGGCAGCTGCAGTAACTGCTGCAGTCTCCTTTCCTT 5 0.125 No Hit GGAATTGGGATGAAGTTATTAGAGAAAATGGGTTATAAGGGAGGTGGATT 5 0.125 No Hit TCGGGTTAATGGAAAATGCTCAAGGTGTAAACGGATGTGGCAGCTCTTAA 5 0.125 No Hit ATAAAAGCTTACAGGCCTTGTCCTGGATTTTTAGCATCCGGTGGAAGGTA 5 0.125 No Hit GGAGCATATAGAAGGTCAAAGAGACCTTATAACTCACAAATCTATATGGC 5 0.125 No Hit AAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAATG 5 0.125 No Hit CATATTTTAGTTGACATTTATGGACTAGCTGGTATGTGATACAGCCTGAG 5 0.125 No Hit CATCGTTGTTGGGAGAGGCAATAACAGGAAAAGGAATTCTATCTCAGTTG 5 0.125 No Hit AAACATTCCGAGCTGGCACTTGAATTCTGTTTTCCAAGAAGAGTTTGATG 5 0.125 No Hit CTCAGAAGAAGATGAAGAAGCAACATTTGTGGAGCCTTCCAGGCGTGGAA 5 0.125 No Hit AACTAAAAAGATAAGATTTCCTCTTCTTCACTTCCTTTCTTTCACTTTTT 5 0.125 No Hit AACACAACAGCCACGAGTTCTTCATCAACACAAAAGCTCTCTCCTTCTCT 5 0.125 No Hit GCCACCAAAAGAGTCTTATATATTTGATGATGCTGGTGTGCTTAGTAGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0125 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.0875 0.0 0.0 0.0 0.0 88-89 0.1125 0.0 0.0 0.0 0.0 90-91 0.25 0.0 0.0 0.0 0.0 92-93 0.275 0.0 0.0 0.0 0.0 94-95 0.3125 0.0 0.0 0.0 0.0 96-97 0.375 0.0 0.0 0.0 0.0 98-99 0.3875 0.0 0.0 0.0 0.0 100-101 0.44999999999999996 0.0 0.0 0.0 0.0 102-103 0.475 0.0 0.0 0.0 0.0 104-105 0.6125 0.0 0.0 0.0 0.0 106-107 0.9375 0.0 0.0 0.0 0.0 108-109 1.25 0.0 0.0 0.0 0.0 110-111 1.625 0.0 0.0 0.0 0.0 112-113 1.7999999999999998 0.0 0.0 0.0 0.0 114-115 2.0375 0.0 0.0 0.0 0.0 116-117 2.3125 0.0 0.0 0.0 0.0 118-119 2.4375 0.0 0.0 0.0 0.0 120-121 2.7625 0.0 0.0 0.0 0.0 122-123 3.1375 0.0 0.0 0.0 0.0 124-125 3.6125 0.0 0.0 0.0 0.0 126-127 3.975 0.0 0.0 0.0 0.0 128-129 4.55 0.0 0.0 0.0 0.0 130-131 5.075 0.0 0.0 0.0 0.0 132-133 5.5625 0.0 0.0 0.0 0.0 134-135 6.1875 0.0 0.0 0.0 0.0 136-137 6.4625 0.0 0.0 0.0 0.0 138-139 7.1375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAAAGT 10 0.006830828 145.0 3 CGGAACC 10 0.006830828 145.0 145 GAGAGTT 10 0.006830828 145.0 2 GTTGGAG 10 0.006830828 145.0 1 GCATGCA 20 0.00593511 29.0 135-139 >>END_MODULE Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931417 spots for SRR13695486.sra Written 931417 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra Read 931404 spots for SRR13695486.sra Written 931404 spots for SRR13695486.sra SRR ids: ['SRR13695486.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_rr2es65b SRR13695486.sra spots: 18628093 blocks: [[1, 931404], [931405, 1862808], [1862809, 2794212], [2794213, 3725616], [3725617, 4657020], [4657021, 5588424], [5588425, 6519828], [6519829, 7451232], [7451233, 8382636], [8382637, 9314040], [9314041, 10245444], [10245445, 11176848], [11176849, 12108252], [12108253, 13039656], [13039657, 13971060], [13971061, 14902464], [14902465, 15833868], [15833869, 16765272], [16765273, 17696676], [17696677, 18628093]] SRR13695486 file size 6308940 SRR13695486 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695486 SRR13695486_1.fastq SRR13695486_2.fastq Input file: SRR13695486_1.fastq Paired file: SRR13695486_2.fastq trimmed: SRR13695486-trimmed-pair1.fastq, SRR13695486-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 07:13:04 2025 >> started Wed Feb 12 07:13:25 2025 >> done (20.506s) 18628093 read pairs processed; of these: 109 ( 0.00%) short read pairs filtered out after trimming by size control 1103 ( 0.01%) empty read pairs filtered out after trimming by size control 18626881 (99.99%) read pairs available; of these: 2177427 (11.69%) trimmed read pairs available after processing 16449454 (88.31%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 2 0.00% 20 2 0.00% 21 3 0.00% 22 2 0.00% 23 3 0.00% 24 6 0.00% 25 1 0.00% 26 3 0.00% 27 2 0.00% 28 2 0.00% 29 1 0.00% 30 2 0.00% 31 7 0.00% 32 7 0.00% 33 4 0.00% 34 6 0.00% 35 3 0.00% 36 3 0.00% 37 3 0.00% 38 11 0.00% 39 11 0.00% 40 12 0.00% 41 13 0.00% 42 9 0.00% 43 17 0.00% 44 7 0.00% 45 14 0.00% 46 11 0.00% 47 36 0.00% 48 28 0.00% 49 48 0.00% 50 33 0.00% 51 37 0.00% 52 59 0.00% 53 59 0.00% 54 83 0.00% 55 78 0.00% 56 94 0.00% 57 92 0.00% 58 112 0.00% 59 148 0.00% 60 171 0.00% 61 208 0.00% 62 253 0.00% 63 265 0.00% 64 276 0.00% 65 413 0.00% 66 422 0.00% 67 477 0.00% 68 504 0.00% 69 719 0.00% 70 730 0.00% 71 934 0.01% 72 981 0.01% 73 1252 0.01% 74 1393 0.01% 75 1486 0.01% 76 1715 0.01% 77 1950 0.01% 78 2351 0.01% 79 2542 0.01% 80 2708 0.01% 81 3141 0.02% 82 3610 0.02% 83 4065 0.02% 84 4635 0.02% 85 5205 0.03% 86 5505 0.03% 87 5900 0.03% 88 6544 0.04% 89 6781 0.04% 90 7542 0.04% 91 8389 0.05% 92 8806 0.05% 93 9769 0.05% 94 10668 0.06% 95 11849 0.06% 96 12249 0.07% 97 12800 0.07% 98 13567 0.07% 99 14419 0.08% 100 15103 0.08% 101 15942 0.09% 102 16911 0.09% 103 17484 0.09% 104 18735 0.10% 105 19847 0.11% 106 20692 0.11% 107 21677 0.12% 108 21647 0.12% 109 23734 0.13% 110 23838 0.13% 111 25027 0.13% 112 25619 0.14% 113 27115 0.15% 114 28032 0.15% 115 29475 0.16% 116 30302 0.16% 117 31551 0.17% 118 32597 0.17% 119 32648 0.18% 120 34041 0.18% 121 35190 0.19% 122 35415 0.19% 123 37029 0.20% 124 37744 0.20% 125 38838 0.21% 126 40509 0.22% 127 41915 0.23% 128 42355 0.23% 129 43792 0.24% 130 44542 0.24% 131 45159 0.24% 132 46179 0.25% 133 46809 0.25% 134 47817 0.26% 135 49443 0.27% 136 50198 0.27% 137 51250 0.28% 138 52188 0.28% 139 54354 0.29% 140 54607 0.29% 141 54569 0.29% 142 55874 0.30% 143 56685 0.30% 144 58049 0.31% 145 58515 0.31% 146 58666 0.31% 147 60770 0.33% 148 61392 0.33% 149 63420 0.34% 150 63868 0.34% 151 16449454 88.31% 18626881 reads passed initial QC criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=22 prefix-density=0.42 prefix-fanout=2.0 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTT criterion=fanout-score sequence-density=0.11 sequence-density-rank=21 fanout-score=9.31 fanout-score-rank=1 prefix-density=0.23 prefix-fanout=4.7 sequence=TTGGCTTGTGCCGGGGTGCGGTTGACGGTGGCAACGGCTGCCGATGAGATCATAGAGGAGGAAGCCAT criterion=sequence-density sequence-density=0.54 sequence-density-rank=1 fanout-score=2.32 fanout-score-rank=22 prefix-density=0.56 prefix-fanout=2.2 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.09 sequence-density-rank=25 fanout-score=30.23 fanout-score-rank=1 prefix-density=0.39 prefix-fanout=7.4 sequence=AAAAAAGAAAGATGGCCTCGGCATCATTTCTCAAGTCATCACCAGTTCTAGACAAGTCTGAGTTTGTTAAGGGTCAGACCCTCCGCTTGCCTTCTGCCTCCATTGTCCGGTGCCGCTCCACCGCCCCTTCTGCTCTTACCGTTCGTGCTGGTTCCTATGCTGAGGAGCTTGTCAAAACCGCGAAAACAATTGCATCTCCTGGCCGAGGTATTTTGGCCATGGATGAGTCTAACGCTACCTGTGGAAAACGTCTCGCCTCAATCGGGCTAGAGAACACCGAGGCTAACCGCCAGGCATACCGTACCCTTCTTGTGACAGTCCCTGGCCTTGGTGATTACGTCTCTGGTGCCATCCTTTTTGAGGAGACTCTCTACCAATCCAC SRR13695486 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 07:14:09 Started mapping on | Feb 12 07:14:09 Finished on | Feb 12 07:16:06 Mapping speed, Million of reads per hour | 573.13 Number of input reads | 18626881 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 17354064 Uniquely mapped reads % | 93.17% Average mapped length | 294.90 Number of splices: Total | 16711676 Number of splices: Annotated (sjdb) | 16361955 Number of splices: GT/AG | 16360937 Number of splices: GC/AG | 286976 Number of splices: AT/AC | 9133 Number of splices: Non-canonical | 54630 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 2.79 Insertion rate per base | 0.02% Insertion average length | 2.38 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 460924 % of reads mapped to multiple loci | 2.47% Number of reads mapped to too many loci | 192928 % of reads mapped to too many loci | 1.04% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.09% % of reads unmapped: other | 0.23% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 812117 812117 812117 N_multimapping 460924 460924 460924 N_noFeature 768158 16946142 1025912 N_ambiguous 264134 1901 112485 UnstrandedReadsAssigned:16321772 PositiveStrandReadsAssigned:406021 NegativeStrandReadsAssigned:16215667 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695486 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695486-trimmed-pair1.fastq SRR13695486-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,626,881 reads, 16,421,078 reads pseudoaligned [quant] estimated average fragment length: 244.499 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,181 rounds 52401 SRR13695486.ke.tsv 34699 SRR13695486.se.tsv 87100 total ==> SRR13695486.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1774.5 548 18.3214 Potri.005G024800.1.v4.1 1035 791.501 338 25.335 Potri.004G059700.1.v4.1 961 717.614 0 0 Potri.007G009000.2.v4.1 1416 1172.5 0 0 Potri.003G141000.2.v4.1 2943 2699.5 862 18.9443 Potri.016G087400.1.v4.1 270 84.708 912 638.742 Potri.015G069301.1.v4.1 564 328.113 0 0 Potri.010G195200.1.v4.1 1773 1529.5 37 1.43518 Potri.012G127500.1.v4.1 977 733.564 69 5.58041 ==> SRR13695486.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 254 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 312 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 5 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1 SRR13695486 completed mapping pipeline successfully