Starting /dee2/code/volunteer_pipeline.sh SRR13695487 current disk space = 3050347982848 free memory = 1289039584 SRR13695487 SRAfilesize 30ee54293905c701810df7511a5f348b SRR13695487.sra SRR13695487.sra file validated SRR13695487 is paired end SRR13695487 is conventional basespace SRR13695487 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695487_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.527 37.0 37.0 37.0 37.0 37.0 2 36.1945 37.0 37.0 37.0 37.0 37.0 3 36.555 37.0 37.0 37.0 37.0 37.0 4 36.4375 37.0 37.0 37.0 37.0 37.0 5 36.581 37.0 37.0 37.0 37.0 37.0 6 36.589 37.0 37.0 37.0 37.0 37.0 7 36.5085 37.0 37.0 37.0 37.0 37.0 8 36.5115 37.0 37.0 37.0 37.0 37.0 9 36.587 37.0 37.0 37.0 37.0 37.0 10-14 36.593599999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.528800000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.517399999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.4901 37.0 37.0 37.0 37.0 37.0 30-34 36.4379 37.0 37.0 37.0 37.0 37.0 35-39 36.4042 37.0 37.0 37.0 37.0 37.0 40-44 36.444900000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.403000000000006 37.0 37.0 37.0 37.0 37.0 50-54 36.3853 37.0 37.0 37.0 37.0 37.0 55-59 36.3488 37.0 37.0 37.0 37.0 37.0 60-64 36.3541 37.0 37.0 37.0 37.0 37.0 65-69 36.318200000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.3287 37.0 37.0 37.0 37.0 37.0 75-79 36.253600000000006 37.0 37.0 37.0 37.0 37.0 80-84 36.190099999999994 37.0 37.0 37.0 37.0 37.0 85-89 36.1953 37.0 37.0 37.0 37.0 37.0 90-94 36.1851 37.0 37.0 37.0 37.0 37.0 95-99 36.15169999999999 37.0 37.0 37.0 37.0 37.0 100-104 36.12779999999999 37.0 37.0 37.0 37.0 37.0 105-109 36.1096 37.0 37.0 37.0 37.0 37.0 110-114 36.052200000000006 37.0 37.0 37.0 37.0 37.0 115-119 36.0409 37.0 37.0 37.0 37.0 37.0 120-124 35.9653 37.0 37.0 37.0 37.0 37.0 125-129 35.871700000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.912 37.0 37.0 37.0 37.0 37.0 135-139 35.7907 37.0 37.0 37.0 37.0 37.0 140-144 35.7341 37.0 37.0 37.0 37.0 37.0 145-149 35.574600000000004 37.0 37.0 37.0 37.0 37.0 150-151 35.490750000000006 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 1.0 25 3.0 26 3.0 27 5.0 28 20.0 29 16.0 30 31.0 31 42.0 32 57.0 33 77.0 34 121.0 35 321.0 36 2950.0 37 352.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.6 12.3 8.525 43.575 2 16.984924623115578 13.040201005025127 39.74874371859297 30.22613065326633 3 17.325 16.725 28.025 37.925 4 23.225 24.625 23.425 28.725 5 23.875 30.8 24.775 20.549999999999997 6 21.15 33.45 24.25 21.15 7 16.150000000000002 27.175 41.349999999999994 15.325 8 17.599999999999998 24.075 34.025 24.3 9 17.724999999999998 23.325000000000003 34.075 24.875 10-14 19.56 29.110000000000003 28.799999999999997 22.53 15-19 19.075 28.38 28.57 23.974999999999998 20-24 19.99 28.299999999999997 27.96 23.75 25-29 19.185 28.689999999999998 28.04 24.085 30-34 19.96 28.405 28.849999999999998 22.785 35-39 19.85 27.834999999999997 28.155 24.16 40-44 19.72 28.860000000000003 28.38 23.04 45-49 19.869999999999997 28.875 27.775 23.48 50-54 19.295 28.915000000000003 28.28 23.51 55-59 20.395 28.439999999999998 28.15 23.015 60-64 20.375 28.205000000000002 27.805000000000003 23.615 65-69 20.36 28.24 28.015 23.385 70-74 20.105 29.39 27.279999999999998 23.225 75-79 20.44 28.87 27.345000000000002 23.345 80-84 20.595 27.36 28.470000000000002 23.575 85-89 21.154999999999998 28.349999999999998 28.189999999999998 22.305 90-94 19.915 28.24 27.994999999999997 23.849999999999998 95-99 20.95 28.560000000000002 28.02 22.470000000000002 100-104 20.0 28.105000000000004 28.225 23.669999999999998 105-109 20.525 28.765 27.66 23.05 110-114 20.919999999999998 27.794999999999998 28.34 22.945 115-119 21.685 28.194999999999997 27.200000000000003 22.919999999999998 120-124 20.735 28.605000000000004 26.724999999999998 23.935000000000002 125-129 20.93 28.03 27.675 23.365 130-134 21.475 27.99 26.99 23.544999999999998 135-139 21.77 28.155 26.889999999999997 23.185 140-144 21.310000000000002 28.16 26.795 23.735 145-149 21.325 28.02 26.915 23.74 150-151 22.9375 28.7 25.2875 23.075000000000003 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.5 18 0.5 19 0.0 20 0.5 21 0.5 22 0.0 23 0.5 24 2.0 25 2.0 26 8.0 27 16.0 28 18.0 29 19.5 30 19.0 31 28.0 32 38.5 33 52.5 34 65.5 35 75.5 36 106.0 37 131.5 38 127.5 39 159.0 40 199.0 41 200.5 42 222.5 43 266.5 44 275.5 45 244.5 46 227.5 47 228.5 48 233.5 49 209.0 50 163.5 51 123.5 52 102.0 53 103.0 54 91.5 55 69.5 56 54.5 57 37.5 58 24.0 59 17.5 60 14.0 61 10.5 62 3.0 63 0.0 64 0.0 65 1.0 66 1.0 67 1.5 68 2.5 69 1.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.425 #Duplication Level Percentage of deduplicated Percentage of total 1 71.6240547353259 49.725 2 18.725243068059054 26.0 3 5.9416636658264315 12.375 4 2.2686352178610014 6.3 5 0.9362621534029528 3.25 6 0.21606049693914295 0.8999999999999999 7 0.21606049693914295 1.05 8 0.07202016564638099 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTAGGAAAGACTTGAACCCAAGCAAAAGTACCTTTGAGATCATTACTCAT 8 0.2 No Hit GCTGATTAGAAAATATTGGAAACTTCCAAGCATCAAAAGTGTTATATTTC 8 0.2 No Hit CTACACAGTAACTATGAAATGGATCCGCATGCAGCATCATATTGTCAACA 7 0.17500000000000002 No Hit CTGCAGAACTAGACATCCTGGACTCCCAGTAACCATGGCTGAATGGGAGT 7 0.17500000000000002 No Hit CGGGTGACTAATCAAAATCTGAGATAAGAACTGCCCTACTGGAGATTCAA 7 0.17500000000000002 No Hit CTTGGGGCTTTGGCCTTTGGCAAGCTCGGAGTACAGTCCCGGATTCTTGT 7 0.17500000000000002 No Hit ACCTGCTCTTCCAAAAACTCGCTTTCGATGAAATCTTGCATTTGTGGATC 7 0.17500000000000002 No Hit GAGGAACTGGTATGATTTCTCCTTTACAGAAAGTAATTGAAATCTGGTAT 7 0.17500000000000002 No Hit ATTTCAATTCCGTGCACAATGACTGGTACCTCCAAAATCCCCGTTTCCAG 6 0.15 No Hit CTGGTATTCTTGATCTCATTGTTAAGTTTGGGGAGGAATCTACATCCTTT 6 0.15 No Hit GCTGCTGTAAGTTCTAACCAAAGAATCTGTTGATCCACCATTAAACAGAA 6 0.15 No Hit GTCCCATCTCAACATGTCACTTAGGCACGCTCACCCCTGATCCTCCTAGC 6 0.15 No Hit GAAGCAAACTTAATATTTATATTATAGAATATTTTTATGGATCCACATGA 6 0.15 No Hit CCCACCTCCAGCAACCAGAACCTTTAACTTTCTTTGCTCCGATTGTTTCC 6 0.15 No Hit GTTTGCTGCATTTGGATCAGTACAGCCTTCAGCGACAGGCACCTTTACTT 5 0.125 No Hit TCCAATTTTCTTGTCTTCCACCTTACTAGGGTCTTCTGGTGGGGTATCAG 5 0.125 No Hit GTTGGTTTGACTCCAAGAAGATACTTGAATTGGGCAACAGTGTAATTGGA 5 0.125 No Hit GTGGGATTGTTGGGTCCATTGTGGATGGGTCAGGTGACCTAGTACCAAAG 5 0.125 No Hit GCACTTGTCGGGTATTATCTTCCCAAATAAGCGAGGTTGAAAATGATACC 5 0.125 No Hit CATGTGTAAATATATAATTTAGATGGTAATGCCTTTTCTTCTCTCCATAA 5 0.125 No Hit GGGAGTATCGTGATAAAGAGAGAGAAAATGAACAAATCGATCGATCTTCG 5 0.125 No Hit CTCATATTTTGTTGGGTCCCTAAGCAAACTAAGATACATGAATTTCTTCG 5 0.125 No Hit GCTCTTGGTCTGGGTGGTGACAGTGACAACGGCGTCTGATTTGATCATTT 5 0.125 No Hit GGTCGTTGTCATCCACATCGTCTAGTTTGCGTTTGGAAGAGACACGGTGA 5 0.125 No Hit AAGGTGTTGGTAGCCAACTGATTGTAGAGGTGATAAAACATTCCAACCCA 5 0.125 No Hit GCTTCCTTCCGGCAGTGTAATCAACCGTGTTTTTGAATTGTAAACTAACT 5 0.125 No Hit GCCAGACTTCTTAGCTTTCTTCTTCTTCCCACCACCCTTCTTCTTTGTCT 5 0.125 No Hit GCCAAACTTAGCGTGATAAAAATTGCATACATATCTGCCATCAGATTTCA 5 0.125 No Hit ATTGCAGCTCTCCTTCCTTCACTTGCTTTCATTTCTCTAGAATCAGCTCT 5 0.125 No Hit ACCCCGTCTGTCAAACTCTTTTACATAGATATTCAAAACAAAAGGTAATC 5 0.125 No Hit CTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTTGGAGGCCAC 5 0.125 No Hit CCGGATTACTTCTCTGAGCTTCACAATATTCGGATGATTCATTTTCCGCA 5 0.125 No Hit GCCGGGATGAAGTGTATCCTTCAAGCTGTCTTATTATGTTCTTTGATACA 5 0.125 No Hit GGACGGCATACATCCTACCCGAATAAGTCCGAACACAGCTATCTTTCTTG 5 0.125 No Hit CTGATGACTTGGAAATGACAGTTGATACTGGAATTGCTAAGAAATCACGA 5 0.125 No Hit GTGTGCAAGGGCAACATGATCATTGCATGCTATGCAATTACCCAAAGCAG 5 0.125 No Hit GGCCATTCAAGATCATCAATAGCTTCCTTCAGCGCTGCAGACTTGACAGT 5 0.125 No Hit GCCCATCTTCAGGAACTCCTGATAGATTGAAGGAAGAGGGTGCTGGGTTC 5 0.125 No Hit CATAAACTTTCAAAGACTCGGTCAATATAATAGAGCAGTCATTATTGTCT 5 0.125 No Hit ATCTAACAGAACATTGTTATACTTCTTAAGAGCAGCAAATGCATCGCTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.0625 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.2375 0.0 0.0 0.0 0.0 84-85 0.2625 0.0 0.0 0.0 0.0 86-87 0.3 0.0 0.0 0.0 0.0 88-89 0.325 0.0 0.0 0.0 0.0 90-91 0.35 0.0 0.0 0.0 0.0 92-93 0.3625 0.0 0.0 0.0 0.0 94-95 0.5125 0.0 0.0 0.0 0.0 96-97 0.7375 0.0 0.0 0.0 0.0 98-99 0.8625 0.0 0.0 0.0 0.0 100-101 0.9874999999999999 0.0 0.0 0.0 0.0 102-103 1.0375 0.0 0.0 0.0 0.0 104-105 1.1875 0.0 0.0 0.0 0.0 106-107 1.3875000000000002 0.0 0.0 0.0 0.0 108-109 1.65 0.0 0.0 0.0 0.0 110-111 2.0 0.0 0.0 0.0 0.0 112-113 2.1375 0.0 0.0 0.0 0.0 114-115 2.4125 0.0 0.0 0.0 0.0 116-117 2.9000000000000004 0.0 0.0 0.0 0.0 118-119 3.2875 0.0 0.0 0.0 0.0 120-121 3.6375 0.0 0.0 0.0 0.0 122-123 3.9375 0.0 0.0 0.0 0.0 124-125 4.425 0.0 0.0 0.0 0.0 126-127 4.762499999999999 0.0 0.0 0.0 0.0 128-129 5.0375 0.0 0.0 0.0 0.0 130-131 5.4375 0.0 0.0 0.0 0.0 132-133 5.7875 0.0 0.0 0.0 0.0 134-135 6.35 0.0 0.0 0.0 0.0 136-137 6.875 0.0 0.0 0.0 0.0 138-139 7.425000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGTCAC 10 0.006830828 145.0 4 CTGGGGT 10 0.006830828 145.0 1 TGGGGTC 10 0.006830828 145.0 2 GGGGTCA 10 0.006830828 145.0 3 >>END_MODULE SRR13695487 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695487_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.19825 37.0 37.0 37.0 37.0 37.0 2 36.0745 37.0 37.0 37.0 37.0 37.0 3 36.0045 37.0 37.0 37.0 37.0 37.0 4 36.121 37.0 37.0 37.0 37.0 37.0 5 36.3435 37.0 37.0 37.0 37.0 37.0 6 36.285 37.0 37.0 37.0 37.0 37.0 7 36.313 37.0 37.0 37.0 37.0 37.0 8 36.3485 37.0 37.0 37.0 37.0 37.0 9 36.3105 37.0 37.0 37.0 37.0 37.0 10-14 36.269600000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.230500000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.19265 37.0 37.0 37.0 37.0 37.0 25-29 36.193250000000006 37.0 37.0 37.0 37.0 37.0 30-34 36.09015000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.074549999999995 37.0 37.0 37.0 37.0 37.0 40-44 36.02184999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.0347 37.0 37.0 37.0 37.0 37.0 50-54 36.00725 37.0 37.0 37.0 37.0 37.0 55-59 35.973549999999996 37.0 37.0 37.0 37.0 37.0 60-64 35.89085 37.0 37.0 37.0 37.0 37.0 65-69 35.893499999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.85635 37.0 37.0 37.0 37.0 37.0 75-79 35.88835 37.0 37.0 37.0 37.0 37.0 80-84 35.897299999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.798899999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.81804999999999 37.0 37.0 37.0 37.0 37.0 95-99 35.82015 37.0 37.0 37.0 37.0 37.0 100-104 35.68814999999999 37.0 37.0 37.0 37.0 37.0 105-109 35.73505 37.0 37.0 37.0 37.0 37.0 110-114 35.65345 37.0 37.0 37.0 37.0 37.0 115-119 35.672250000000005 37.0 37.0 37.0 37.0 37.0 120-124 35.568349999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.565999999999995 37.0 37.0 37.0 37.0 37.0 130-134 35.50015 37.0 37.0 37.0 37.0 37.0 135-139 35.43925 37.0 37.0 37.0 37.0 37.0 140-144 35.26695 37.0 37.0 37.0 34.6 37.0 145-149 35.223400000000005 37.0 37.0 37.0 32.2 37.0 150-151 35.040625 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 5.0 15 3.0 16 1.0 17 1.0 18 2.0 19 2.0 20 2.0 21 1.0 22 1.0 23 4.0 24 6.0 25 5.0 26 7.0 27 11.0 28 15.0 29 21.0 30 16.0 31 44.0 32 63.0 33 121.0 34 253.0 35 585.0 36 2619.0 37 212.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.37515683814304 21.580928481806776 13.400250941028858 31.64366373902133 2 26.55 25.6 31.55 16.3 3 19.825 27.250000000000004 32.725 20.200000000000003 4 23.325000000000003 32.6 25.224999999999998 18.85 5 27.400000000000002 37.175000000000004 21.675 13.750000000000002 6 20.150000000000002 39.525 21.85 18.475 7 20.3 21.9 39.85 17.95 8 20.150000000000002 25.8 29.925 24.125 9 21.825 24.25 31.275 22.650000000000002 10-14 22.705000000000002 29.459999999999997 27.11 20.724999999999998 15-19 22.14 28.625 27.625 21.61 20-24 22.296114805740284 28.181409070453523 28.376418820941048 21.14605730286514 25-29 22.655663915978995 27.831957989497376 28.632158039509875 20.880220055013755 30-34 21.87328099214882 28.31424713707056 28.939340901135168 20.873130969645448 35-39 22.536126806340317 28.756437821891094 27.636381819090953 21.071053552677636 40-44 22.24333650047507 28.52427864179627 27.489123368505275 21.743261489223382 45-49 22.887288728872885 28.412841284128415 28.08780878087809 20.612061206120615 50-54 22.186109305465273 28.841442072103607 28.331416570828544 20.641032051602583 55-59 22.315578894723682 27.62690672668167 28.362090522630655 21.69542385596399 60-64 23.401170058502927 27.42137106855343 28.146407320366016 21.03105155257763 65-69 22.8 27.21 28.775000000000002 21.215 70-74 23.080770192548137 27.481870467616904 28.197049262315577 21.240310077519382 75-79 22.12831924788718 27.394109116367453 28.104215632344854 22.37335600340051 80-84 23.53 28.325 26.640000000000004 21.505 85-89 23.344668933786757 27.91058211642328 27.930586117223445 20.814162832566513 90-94 23.53117655882794 27.266363318165908 27.74138706935347 21.461073053652683 95-99 23.67618380919046 28.0114005700285 27.76638831941597 20.54602730136507 100-104 23.37584396099025 28.257064266066518 27.371842960740185 20.99524881220305 105-109 23.380845211302827 27.991997999499873 28.032008002000502 20.5951487871968 110-114 22.651132556627832 28.456422821141057 27.65638281914096 21.236061803090152 115-119 23.91097774443611 28.33208302075519 27.401850462615652 20.355088772193046 120-124 23.195798949737434 29.422355588897226 26.76169042260565 20.62015503875969 125-129 23.854770954190837 28.880776155231047 26.67033406681336 20.594118823764752 130-134 24.90120554249412 28.032614676604474 26.842078935520984 20.22410084538042 135-139 24.478567498624518 27.70469664382534 27.719701895663484 20.09703396188666 140-144 25.12625631281564 27.701385069253465 27.13135656782839 20.041002050102506 145-149 25.407622286686006 28.848654596378914 26.272881864559366 19.470841252375713 150-151 27.204502814258912 28.355222013758596 25.86616635397123 18.574108818011258 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.5 14 2.5 15 3.5 16 2.0 17 1.5 18 1.0 19 0.0 20 0.0 21 0.0 22 0.0 23 1.5 24 2.0 25 2.5 26 6.5 27 9.0 28 8.5 29 16.5 30 31.5 31 31.0 32 28.0 33 42.5 34 50.5 35 56.5 36 84.0 37 116.5 38 151.0 39 150.5 40 163.0 41 235.0 42 258.5 43 258.5 44 274.5 45 271.0 46 253.0 47 246.5 48 226.5 49 201.5 50 176.5 51 136.5 52 117.0 53 98.0 54 75.5 55 52.0 56 43.5 57 36.5 58 19.5 59 11.0 60 8.5 61 9.0 62 7.0 63 5.5 64 4.0 65 1.5 66 0.0 67 1.5 68 2.0 69 1.5 70 1.0 71 0.5 72 0.5 73 0.0 74 0.0 75 0.5 76 0.5 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.015 35-39 0.005 40-44 0.015 45-49 0.01 50-54 0.005 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.015 80-84 0.0 85-89 0.02 90-94 0.005 95-99 0.005 100-104 0.025 105-109 0.025 110-114 0.005 115-119 0.025 120-124 0.025 125-129 0.02 130-134 0.045 135-139 0.034999999999999996 140-144 0.005 145-149 0.03 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.075 #Duplication Level Percentage of deduplicated Percentage of total 1 72.67213699607564 50.925 2 18.08776311095255 25.35 3 5.672493756689261 11.924999999999999 4 2.1405636817695326 6.0 5 0.8919015340706385 3.125 6 0.2497324295397788 1.05 7 0.17838030681412773 0.8750000000000001 8 0.03567606136282554 0.2 9 0.03567606136282554 0.22499999999999998 >10 0.03567606136282554 0.325 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 13 0.325 No Hit ATTTGAGCATGTTGAGAAAGGAGATGCACTATATGCTATGGAACTGGCAT 9 0.22499999999999998 No Hit CTAGGATGCAATTCTTCCTCATTGCCATGACAGCAAGCTTTCTCTATTAT 8 0.2 No Hit TGGTGTTGAAGAGGAAGAAAGACTATGAGGAGGCATCGGAAGAGAATGCT 7 0.17500000000000002 No Hit ATTTTGTGCGTTTTCCTCATAAGCATTGTTCTTCGGTTTCATACTCGAAG 7 0.17500000000000002 No Hit GAGCTGAAAACTGTAGCAGCTGCGAAAGTGGAGCAGATAACAGCTGAATT 7 0.17500000000000002 No Hit ACTGAATGGGTGAAAGGGAAGCAAATGGAGGAAGTGATGACCATAAAAAA 7 0.17500000000000002 No Hit GCGAACCCTTGAAGAGGCATCAGCTTCCATGGCAGCTGCAATGGAAGAAG 7 0.17500000000000002 No Hit AAAAGTTATATTGGATTAAGAAGTAGCTAATTGCAAACAATTTGTTTTGT 6 0.15 No Hit CTGCGTTTCCAGAGCCATGCTGTGCTGGCGCTGCAAGAGGCAGCTGAGGC 6 0.15 No Hit AATCGGGCAAGCAAGGTGTACAAGCTTTAGAGCTCGCATATACAATGAGA 6 0.15 No Hit CACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATC 6 0.15 No Hit GGCATTTCGTTTGGCCTTTGGACGATCAAGTCTCTTCTTTTGCTCATATG 6 0.15 No Hit TCAAAACCAAGACAAGTACTAGTGCTAAGAAATTGAAAGTGAAAGCTGTA 6 0.15 No Hit CGATACATCTGTTGGTCGAGTTGGTAATCCAGTCCCTTGCTCATATATTA 6 0.15 No Hit AGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCCTCTGTGATT 5 0.125 No Hit CGGGGAGAACACAATCATCACCGTTCATCAAATGTCTCTTCCCCAATCAC 5 0.125 No Hit CTGAGATCGTTTTCGCTCAGGTCAAGATATTCAAGAGAAAAAGAGGTTAA 5 0.125 No Hit AATTCCCAAGGAAGATATTGTCAAGCTTGTTGATACCTTCCCTGGTCAAT 5 0.125 No Hit AGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 5 0.125 No Hit GTTGCTTCTCATCGGTGCCATCTTTACCTTTCAGTCTCAGGTTATTGCAG 5 0.125 No Hit AGCAACCTGAGGTACCAGATCCTTGCTGGAGTTATTGAACAGCGACTTTT 5 0.125 No Hit ATCCAGTGTAAACTCTCACTGGCAAGAAGCTAGTGTGTGTACTTCATTGT 5 0.125 No Hit ATCTTCTTCTGCCATGGCGGACGTGAGTCGTTGTACATCATCTGATATTA 5 0.125 No Hit CATGAATATTCAGTTATATAATCACAATATCACGGTTTCACGAATTGCCA 5 0.125 No Hit CAGGTGGAAAGAGTTCGTCAAATGATTCTGTTACTAGTGAGGCATGGTTT 5 0.125 No Hit CTCAGTTTTGTAATAACACATTTTGCAAGCAATGGCAGCCATGAATTCTA 5 0.125 No Hit GTCACACAAACACTAATCCAGTACAAAAAATGCTCCAAACTCTATCCACC 5 0.125 No Hit GGACTTTTTGATCTTGAAGGGGCCCTGCCCCAACTGTGGCACGGAGAACG 5 0.125 No Hit AATTTGCAAAAGAAATTCCTGATATTAAAAGAAGAAGAGCACTCACAAGA 5 0.125 No Hit GTTTGTTCTTTTGAGTTGTTGGGCTAAAAAATAAGCGATATGTGCCTTTG 5 0.125 No Hit CACATTTTCGGTTCCTGGGCATTCCAGCACTATTGAAATTCGATACCCTG 5 0.125 No Hit GGTGACTTGAGAAAATATTTGTCTGGATTCTTTGACACCACAGTGGGGAA 5 0.125 No Hit ATTTGCGAAGGGCGTACAAGCCAGTTGGATTAGTGTTGATTTTGGTGAAC 5 0.125 No Hit AAACAATTCAGCAAAATCAAGACAAAAATAACAATTCTGATGACAAGAGT 5 0.125 No Hit ATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAAT 5 0.125 No Hit ATTTGTGTCTGTGTTCAAAGGGAAGAGTCCCCTGATTATGCAGCTTCACT 5 0.125 No Hit CCAAGAGGAGTGCTAGGGACTCCCATGTGCCTGTATTGGCTCCTCTCCCA 5 0.125 No Hit GCAAGAGCTCTCGAAGAGAAAAGGGCTCGGCTGGGATTATTAGAATGTGT 5 0.125 No Hit GTTTGCCTTGGCAGCATGATCTGCTTGAGGATAGCATTAGAGCTGCAGGA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.0625 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.2375 0.0 0.0 0.0 0.0 84-85 0.2625 0.0 0.0 0.0 0.0 86-87 0.3 0.0 0.0 0.0 0.0 88-89 0.325 0.0 0.0 0.0 0.0 90-91 0.35 0.0 0.0 0.0 0.0 92-93 0.3625 0.0 0.0 0.0 0.0 94-95 0.5125 0.0 0.0 0.0 0.0 96-97 0.7375 0.0 0.0 0.0 0.0 98-99 0.8625 0.0 0.0 0.0 0.0 100-101 0.9874999999999999 0.0 0.0 0.0 0.0 102-103 1.0375 0.0 0.0 0.0 0.0 104-105 1.1749999999999998 0.0 0.0 0.0 0.0 106-107 1.35 0.0 0.0 0.0 0.0 108-109 1.6 0.0 0.0 0.0 0.0 110-111 1.95 0.0 0.0 0.0 0.0 112-113 2.0875 0.0 0.0 0.0 0.0 114-115 2.3625 0.0 0.0 0.0 0.0 116-117 2.8499999999999996 0.0 0.0 0.0 0.0 118-119 3.2375 0.0 0.0 0.0 0.0 120-121 3.5875 0.0 0.0 0.0 0.0 122-123 3.8875 0.0 0.0 0.0 0.0 124-125 4.325 0.0 0.0 0.0 0.0 126-127 4.612500000000001 0.0 0.0 0.0 0.0 128-129 4.887499999999999 0.0 0.0 0.0 0.0 130-131 5.2875 0.0 0.0 0.0 0.0 132-133 5.65 0.0 0.0 0.0 0.0 134-135 6.225 0.0 0.0 0.0 0.0 136-137 6.7375 0.0 0.0 0.0 0.0 138-139 7.275 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGATGT 10 0.006830828 145.0 3 GATGTTG 10 0.006830828 145.0 5 GGATGAT 20 3.5877043E-4 108.75 1 >>END_MODULE Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra Read 890277 spots for SRR13695487.sra Written 890277 spots for SRR13695487.sra Read 890274 spots for SRR13695487.sra Written 890274 spots for SRR13695487.sra SRR ids: ['SRR13695487.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_fe9wlj3e SRR13695487.sra spots: 17805483 blocks: [[1, 890274], [890275, 1780548], [1780549, 2670822], [2670823, 3561096], [3561097, 4451370], [4451371, 5341644], [5341645, 6231918], [6231919, 7122192], [7122193, 8012466], [8012467, 8902740], [8902741, 9793014], [9793015, 10683288], [10683289, 11573562], [11573563, 12463836], [12463837, 13354110], [13354111, 14244384], [14244385, 15134658], [15134659, 16024932], [16024933, 16915206], [16915207, 17805483]] SRR13695487 file size 6029381 SRR13695487 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695487 SRR13695487_1.fastq SRR13695487_2.fastq Input file: SRR13695487_1.fastq Paired file: SRR13695487_2.fastq trimmed: SRR13695487-trimmed-pair1.fastq, SRR13695487-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 06:29:41 2025 >> started Wed Feb 12 06:30:16 2025 >> done (34.940s) 17805483 read pairs processed; of these: 124 ( 0.00%) short read pairs filtered out after trimming by size control 1327 ( 0.01%) empty read pairs filtered out after trimming by size control 17804032 (99.99%) read pairs available; of these: 2054085 (11.54%) trimmed read pairs available after processing 15749947 (88.46%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 20 1 0.00% 21 1 0.00% 22 1 0.00% 23 2 0.00% 24 2 0.00% 25 2 0.00% 26 2 0.00% 27 8 0.00% 28 3 0.00% 29 2 0.00% 30 4 0.00% 31 0 0.00% 32 14 0.00% 33 4 0.00% 34 4 0.00% 35 7 0.00% 36 5 0.00% 37 1 0.00% 38 6 0.00% 39 17 0.00% 40 6 0.00% 41 9 0.00% 42 11 0.00% 43 11 0.00% 44 13 0.00% 45 10 0.00% 46 15 0.00% 47 19 0.00% 48 49 0.00% 49 25 0.00% 50 36 0.00% 51 33 0.00% 52 75 0.00% 53 58 0.00% 54 80 0.00% 55 57 0.00% 56 89 0.00% 57 88 0.00% 58 134 0.00% 59 159 0.00% 60 147 0.00% 61 204 0.00% 62 228 0.00% 63 328 0.00% 64 295 0.00% 65 361 0.00% 66 443 0.00% 67 451 0.00% 68 499 0.00% 69 634 0.00% 70 808 0.00% 71 858 0.00% 72 997 0.01% 73 1217 0.01% 74 1327 0.01% 75 1492 0.01% 76 1738 0.01% 77 1922 0.01% 78 2190 0.01% 79 2433 0.01% 80 2709 0.02% 81 2959 0.02% 82 3665 0.02% 83 4009 0.02% 84 4521 0.03% 85 5136 0.03% 86 5296 0.03% 87 5711 0.03% 88 6338 0.04% 89 6796 0.04% 90 7481 0.04% 91 8272 0.05% 92 8700 0.05% 93 9455 0.05% 94 10205 0.06% 95 10950 0.06% 96 11479 0.06% 97 12241 0.07% 98 12930 0.07% 99 13827 0.08% 100 14706 0.08% 101 15180 0.09% 102 15829 0.09% 103 16656 0.09% 104 17546 0.10% 105 18849 0.11% 106 19828 0.11% 107 20370 0.11% 108 21216 0.12% 109 22124 0.12% 110 22663 0.13% 111 23257 0.13% 112 24590 0.14% 113 24903 0.14% 114 26345 0.15% 115 27660 0.16% 116 28511 0.16% 117 29155 0.16% 118 31077 0.17% 119 31306 0.18% 120 32252 0.18% 121 33098 0.19% 122 34321 0.19% 123 35349 0.20% 124 36210 0.20% 125 36223 0.20% 126 38388 0.22% 127 39304 0.22% 128 40413 0.23% 129 40920 0.23% 130 41996 0.24% 131 42361 0.24% 132 43044 0.24% 133 45053 0.25% 134 44180 0.25% 135 45644 0.26% 136 46968 0.26% 137 48341 0.27% 138 48101 0.27% 139 50559 0.28% 140 51290 0.29% 141 51729 0.29% 142 53034 0.30% 143 52462 0.29% 144 54610 0.31% 145 54836 0.31% 146 55528 0.31% 147 57002 0.32% 148 58510 0.33% 149 58554 0.33% 150 59719 0.34% 151 15749947 88.46% 17804032 reads passed initial QC criterion=sequence-density sequence-density=0.73 sequence-density-rank=1 fanout-score=2.09 fanout-score-rank=21 prefix-density=0.77 prefix-fanout=2.0 sequence=TTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=27 fanout-score=278.09 fanout-score-rank=1 prefix-density=0.24 prefix-fanout=16.0 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTG criterion=sequence-density sequence-density=0.98 sequence-density-rank=1 fanout-score=2.21 fanout-score-rank=21 prefix-density=1.01 prefix-fanout=2.1 sequence=CCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=16.50 fanout-score-rank=1 prefix-density=0.03 prefix-fanout=2.9 sequence=TAAACCCAAAGCTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAATGGCCCTCTC SRR13695487 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 06:30:55 Started mapping on | Feb 12 06:30:55 Finished on | Feb 12 06:33:00 Mapping speed, Million of reads per hour | 512.76 Number of input reads | 17804032 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 16681624 Uniquely mapped reads % | 93.70% Average mapped length | 295.05 Number of splices: Total | 16357819 Number of splices: Annotated (sjdb) | 15987243 Number of splices: GT/AG | 16021734 Number of splices: GC/AG | 260872 Number of splices: AT/AC | 10281 Number of splices: Non-canonical | 64932 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.88 Insertion rate per base | 0.02% Insertion average length | 2.40 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 458059 % of reads mapped to multiple loci | 2.57% Number of reads mapped to too many loci | 76222 % of reads mapped to too many loci | 0.43% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.19% % of reads unmapped: other | 0.11% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 664557 664557 664557 N_multimapping 458059 458059 458059 N_noFeature 724796 16193221 1020429 N_ambiguous 304648 2140 110109 UnstrandedReadsAssigned:15652180 PositiveStrandReadsAssigned:486263 NegativeStrandReadsAssigned:15551086 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695487 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695487-trimmed-pair1.fastq SRR13695487-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,804,032 reads, 15,563,943 reads pseudoaligned [quant] estimated average fragment length: 250.998 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,218 rounds 52401 SRR13695487.ke.tsv 34699 SRR13695487.se.tsv 87100 total ==> SRR13695487.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1768 728 21.8725 Potri.005G024800.1.v4.1 1035 785.002 456 30.8563 Potri.004G059700.1.v4.1 961 711.084 7 0.522909 Potri.007G009000.2.v4.1 1416 1166 0 0 Potri.003G141000.2.v4.1 2943 2693 767.876 15.1462 Potri.016G087400.1.v4.1 270 84.8168 907 568.035 Potri.015G069301.1.v4.1 564 324.239 0 0 Potri.010G195200.1.v4.1 1773 1523 131 4.56899 Potri.012G127500.1.v4.1 977 727.028 403 29.4445 ==> SRR13695487.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 153 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 325 Potri.001G212900.v4.1 188 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 20 Potri.001G416900.v4.1 4 Potri.001G452600.v4.1 14 SRR13695487 completed mapping pipeline successfully