Starting /dee2/code/volunteer_pipeline.sh SRR13695489 current disk space = 3049971429376 free memory = 1577582128 SRR13695489 SRAfilesize cb0f94d07e17ed5860bdcfb88b70cc60 SRR13695489.sra SRR13695489.sra file validated SRR13695489 is paired end SRR13695489 is conventional basespace SRR13695489 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695489_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5655 37.0 37.0 37.0 37.0 37.0 2 36.37125 37.0 37.0 37.0 37.0 37.0 3 36.57 37.0 37.0 37.0 37.0 37.0 4 36.4615 37.0 37.0 37.0 37.0 37.0 5 36.56 37.0 37.0 37.0 37.0 37.0 6 36.527 37.0 37.0 37.0 37.0 37.0 7 36.5445 37.0 37.0 37.0 37.0 37.0 8 36.654 37.0 37.0 37.0 37.0 37.0 9 36.5665 37.0 37.0 37.0 37.0 37.0 10-14 36.592 37.0 37.0 37.0 37.0 37.0 15-19 36.545100000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.5193 37.0 37.0 37.0 37.0 37.0 25-29 36.471999999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.4898 37.0 37.0 37.0 37.0 37.0 35-39 36.4669 37.0 37.0 37.0 37.0 37.0 40-44 36.478699999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.4265 37.0 37.0 37.0 37.0 37.0 50-54 36.4268 37.0 37.0 37.0 37.0 37.0 55-59 36.3893 37.0 37.0 37.0 37.0 37.0 60-64 36.425599999999996 37.0 37.0 37.0 37.0 37.0 65-69 36.349199999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.3618 37.0 37.0 37.0 37.0 37.0 75-79 36.335300000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.285 37.0 37.0 37.0 37.0 37.0 85-89 36.28 37.0 37.0 37.0 37.0 37.0 90-94 36.2017 37.0 37.0 37.0 37.0 37.0 95-99 36.2019 37.0 37.0 37.0 37.0 37.0 100-104 36.1999 37.0 37.0 37.0 37.0 37.0 105-109 36.1463 37.0 37.0 37.0 37.0 37.0 110-114 36.1785 37.0 37.0 37.0 37.0 37.0 115-119 36.1289 37.0 37.0 37.0 37.0 37.0 120-124 36.0948 37.0 37.0 37.0 37.0 37.0 125-129 36.0647 37.0 37.0 37.0 37.0 37.0 130-134 36.0419 37.0 37.0 37.0 37.0 37.0 135-139 35.876799999999996 37.0 37.0 37.0 37.0 37.0 140-144 35.751999999999995 37.0 37.0 37.0 37.0 37.0 145-149 35.545500000000004 37.0 37.0 37.0 37.0 37.0 150-151 35.278000000000006 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 2.0 25 4.0 26 2.0 27 10.0 28 8.0 29 18.0 30 22.0 31 34.0 32 48.0 33 71.0 34 126.0 35 314.0 36 3025.0 37 316.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.75 12.225 9.025 42.0 2 19.723964868255962 15.056461731493098 36.235884567126725 28.983688833124216 3 17.325 18.099999999999998 27.625 36.95 4 21.95 24.325 24.3 29.425 5 24.275 30.9 24.25 20.575 6 20.599999999999998 34.825 23.974999999999998 20.599999999999998 7 15.8 27.400000000000002 41.099999999999994 15.7 8 18.224999999999998 26.450000000000003 31.900000000000002 23.425 9 17.299999999999997 23.425 36.025 23.25 10-14 19.865 30.12 27.96 22.055 15-19 20.075000000000003 27.41 29.23 23.285 20-24 20.424999999999997 28.32 27.97 23.285 25-29 20.424999999999997 28.449999999999996 28.16 22.965 30-34 20.01 28.660000000000004 27.894999999999996 23.435 35-39 20.724999999999998 28.735 27.22 23.32 40-44 20.055 28.43 28.105000000000004 23.41 45-49 19.830000000000002 28.62 28.384999999999998 23.165 50-54 20.315 28.549999999999997 28.110000000000003 23.025000000000002 55-59 20.424999999999997 28.63 28.1 22.845 60-64 20.630000000000003 29.285 26.715 23.369999999999997 65-69 20.0 28.79 28.325 22.884999999999998 70-74 20.669999999999998 29.435 27.145000000000003 22.75 75-79 20.195 27.500000000000004 28.64 23.665 80-84 19.915 28.720000000000002 27.675 23.69 85-89 20.535 28.49 27.544999999999998 23.43 90-94 20.445 28.155 28.405 22.994999999999997 95-99 20.165 27.689999999999998 28.4 23.745 100-104 20.36 28.725 27.034999999999997 23.880000000000003 105-109 21.305 28.01 27.575 23.11 110-114 20.86 28.15 27.860000000000003 23.13 115-119 20.075000000000003 28.765 27.58 23.580000000000002 120-124 20.53 28.410000000000004 27.77 23.29 125-129 20.674999999999997 28.725 27.060000000000002 23.54 130-134 20.905 28.67 25.94 24.485 135-139 22.11 28.384999999999998 26.229999999999997 23.275000000000002 140-144 20.87 28.68 27.105 23.345 145-149 21.175 28.449999999999996 26.590000000000003 23.785 150-151 20.7625 29.049999999999997 26.3 23.8875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 1.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.5 23 1.5 24 4.5 25 7.0 26 6.5 27 5.0 28 6.5 29 15.5 30 23.5 31 35.0 32 41.0 33 41.0 34 56.5 35 78.5 36 94.5 37 112.5 38 138.5 39 165.0 40 190.0 41 205.0 42 229.0 43 255.0 44 251.5 45 268.0 46 282.0 47 255.0 48 239.0 49 212.5 50 168.5 51 136.0 52 117.0 53 91.0 54 68.0 55 56.5 56 39.0 57 27.0 58 20.5 59 14.0 60 10.5 61 9.0 62 3.5 63 3.5 64 3.5 65 2.5 66 2.0 67 1.0 68 2.0 69 1.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.375 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.525 #Duplication Level Percentage of deduplicated Percentage of total 1 70.01094491061657 47.975 2 19.445457862094127 26.650000000000002 3 6.968259759211966 14.325 4 2.225465158701204 6.1 5 0.9850419554906968 3.375 6 0.2553812477198103 1.05 7 0.10944910616563297 0.525 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTCTATGGTACAATTAAATATACATTCCCTTTTTTTCACAGTATGTGCA 7 0.17500000000000002 No Hit CAAAACTTTAAGAGCATGCAATGCTCCAATGTTCTGGAGAAAGTTTACTG 7 0.17500000000000002 No Hit ATCAGATTTTACACCTCCTCTGATACTGATGTAAATATTTTCTTCATCAG 7 0.17500000000000002 No Hit CCAAATAATCAATTGAGTACCTTGGCACTATTACTCCGTAAATCTTTCTT 6 0.15 No Hit CCCAACTTCCCACCAGAGCATCAGAACCAATATTCGCCTCTAAGATGTCA 6 0.15 No Hit GACAGTTTTAGTTCCTTGAACTCCATACTCAGCTTCACCAAAGGAGTCCT 6 0.15 No Hit GCCTATTCAGTGGGTGAGTATTGGACTCGATCTCCGAAGTTACATCAGGT 6 0.15 No Hit ACATCCTTCATGAGGGCATTTCGGCATCATTCCATGGAGCAATTTAACCT 6 0.15 No Hit AATGGGGATTGAGACAAGTGTGGCTAATAAGAAAGTAAAACAAGTTGGTG 6 0.15 No Hit GCCGCGAGAGGCATCCGGTTGAACCAAAAGAATCATGACGACGTTCGGCA 6 0.15 No Hit TAGAGTTAGATTTACGCCAGTCAAGGAAGCTTCCAACCTCACTTTTTTCC 5 0.125 No Hit GGGAAAGGAGCCAAAACTTGTGGGCTCAACCTGAAAATAGCAATGGAAGG 5 0.125 No Hit CCCGGCATTGTTCTTGAAGACAATCTTTTCACCAGCGGGTACAGAGAATT 5 0.125 No Hit CCGCAAACAAATAACCAACAACAAAGGTAAAAAAACAAAAAAACAAAGAG 5 0.125 No Hit TCTTCTCTTGATAAAAAACCAAAAAATTGTAGCAGCAGAAACAACAAGAA 5 0.125 No Hit GGCGAGCACGAGGATGGTCCCAATCGGCTACAATGTGGTCACGAGTGATT 5 0.125 No Hit GGCTTAACAACTATACAAATAATACAATTTTCAATCAAAACTGAGAAATC 5 0.125 No Hit GTAAATGTTTTTGGTGGCCAACCAAGGCTACAAAGTCAACTATGCATTTT 5 0.125 No Hit CTTCAGTCATTTCTACAATAGCAAACGGGGTGCAGTCCATCGACTTGTAA 5 0.125 No Hit TCTTATACTTGTTTGAATATGTCATGTTCTTCATTGCGGATCGGCTTTCC 5 0.125 No Hit GTTGTTTATGTATGCTTTGGAAGTATAGCCAACTTTGCTGCTTCACAACT 5 0.125 No Hit CCTATAGGTTCGAAGATTGTGTAAAGAGCACCTGTGCTTACAGTTAATGC 5 0.125 No Hit CACAGCCATTAAATTACACAAAAATCAAAACATCATCACAGTACAAAACA 5 0.125 No Hit CCTTCTTATTGGCTTTCATTGGATGGGAGGCCAGGGCTCTCACTCCAATA 5 0.125 No Hit TGAAAAACAATAGCAGGGGCAGAAGGATAGCCATGAGAAGCCATCAAACA 5 0.125 No Hit CTCGAATGACAGCTACTTTTGGTTTCGAAGTTGCAATCATATATTTCTCA 5 0.125 No Hit CCCTAATCACGTCAAAGTTCAACAAACTGATCCCAGTTTCTTCCTTCATT 5 0.125 No Hit CTCCAATAAGATACTCCATAACGAGATACAGGTTATCTCTGCATGTGAAT 5 0.125 No Hit GCAACGAAGAAGGGTTTTGGGGTCTCCAGTTTTGTTCAAGATGGTTATGA 5 0.125 No Hit CGGGGGGAAAAAACCAAAAATCTCTATCCAAGAAAAGTGTGAACCAATAT 5 0.125 No Hit CCCCAAGTTCTTACAAGGATATCATAGAGGACAATAAGCTTAATTAACAT 5 0.125 No Hit AATGGATGGTGGCTCACCATTATTCATGTACAGCTGGTTATCGTGGGCTA 5 0.125 No Hit GCTAAAGCTCTAGTATCTTCTAGCATCTTTTCACAATCCTTGAATCTCTT 5 0.125 No Hit CCACTTCCTCACCTCATCGTCATAAACTCGGGCCCTGAGAGCACCAAAGA 5 0.125 No Hit CCAATGGTGACTCATCAATCTTCCAGTCCCCAGTGTGAAGAATTGTACCA 5 0.125 No Hit CTCCTTTCCACTCGTATGGTTAATCAACTTACTTTCCGCAAGTTCAATCG 5 0.125 No Hit GGCTCTATTCAGTTCCCAGCAGCCAAGAAAGATTTTTTCTGTTTATCGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0125 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.125 0.0 0.0 0.0 0.0 84-85 0.2 0.0 0.0 0.0 0.0 86-87 0.225 0.0 0.0 0.0 0.0 88-89 0.25 0.0 0.0 0.0 0.0 90-91 0.3875 0.0 0.0 0.0 0.0 92-93 0.5375000000000001 0.0 0.0 0.0 0.0 94-95 0.6875 0.0 0.0 0.0 0.0 96-97 0.875 0.0 0.0 0.0 0.0 98-99 1.1375 0.0 0.0 0.0 0.0 100-101 1.4874999999999998 0.0 0.0 0.0 0.0 102-103 1.5875 0.0 0.0 0.0 0.0 104-105 1.8 0.0 0.0 0.0 0.0 106-107 2.0999999999999996 0.0 0.0 0.0 0.0 108-109 2.4 0.0 0.0 0.0 0.0 110-111 2.5625 0.0 0.0 0.0 0.0 112-113 2.8125 0.0 0.0 0.0 0.0 114-115 3.0125 0.0 0.0 0.0 0.0 116-117 3.425 0.0 0.0 0.0 0.0 118-119 3.7875 0.0 0.0 0.0 0.0 120-121 3.9625 0.0 0.0 0.0 0.0 122-123 4.3375 0.0 0.0 0.0 0.0 124-125 4.699999999999999 0.0 0.0 0.0 0.0 126-127 5.25 0.0 0.0 0.0 0.0 128-129 5.55 0.0 0.0 0.0 0.0 130-131 5.925000000000001 0.0 0.0 0.0 0.0 132-133 6.262499999999999 0.0 0.0 0.0 0.0 134-135 6.7875 0.0 0.0 0.0 0.0 136-137 7.4625 0.0 0.0 0.0 0.0 138-139 8.2875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTGAT 10 0.006830828 145.0 1 GAAAAAT 10 0.006830828 145.0 8 TGAAAAA 10 0.006830828 145.0 7 >>END_MODULE SRR13695489 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695489_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.088 37.0 37.0 37.0 37.0 37.0 2 36.2385 37.0 37.0 37.0 37.0 37.0 3 36.3765 37.0 37.0 37.0 37.0 37.0 4 36.332 37.0 37.0 37.0 37.0 37.0 5 36.385 37.0 37.0 37.0 37.0 37.0 6 36.3175 37.0 37.0 37.0 37.0 37.0 7 36.4675 37.0 37.0 37.0 37.0 37.0 8 36.462 37.0 37.0 37.0 37.0 37.0 9 36.3655 37.0 37.0 37.0 37.0 37.0 10-14 36.3378 37.0 37.0 37.0 37.0 37.0 15-19 36.3289 37.0 37.0 37.0 37.0 37.0 20-24 36.3415 37.0 37.0 37.0 37.0 37.0 25-29 36.242200000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.211349999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.2105 37.0 37.0 37.0 37.0 37.0 40-44 36.18235 37.0 37.0 37.0 37.0 37.0 45-49 36.160199999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.12615 37.0 37.0 37.0 37.0 37.0 55-59 36.1146 37.0 37.0 37.0 37.0 37.0 60-64 36.0779 37.0 37.0 37.0 37.0 37.0 65-69 36.04505 37.0 37.0 37.0 37.0 37.0 70-74 36.0221 37.0 37.0 37.0 37.0 37.0 75-79 36.054899999999996 37.0 37.0 37.0 37.0 37.0 80-84 36.035849999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.95595 37.0 37.0 37.0 37.0 37.0 90-94 35.963800000000006 37.0 37.0 37.0 37.0 37.0 95-99 35.93885 37.0 37.0 37.0 37.0 37.0 100-104 35.8699 37.0 37.0 37.0 37.0 37.0 105-109 35.876850000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.8172 37.0 37.0 37.0 37.0 37.0 115-119 35.787400000000005 37.0 37.0 37.0 37.0 37.0 120-124 35.7322 37.0 37.0 37.0 37.0 37.0 125-129 35.75255 37.0 37.0 37.0 37.0 37.0 130-134 35.65 37.0 37.0 37.0 37.0 37.0 135-139 35.58105 37.0 37.0 37.0 37.0 37.0 140-144 35.468 37.0 37.0 37.0 37.0 37.0 145-149 35.3457 37.0 37.0 37.0 34.6 37.0 150-151 35.145125 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 0.0 14 3.0 15 2.0 16 4.0 17 2.0 18 0.0 19 1.0 20 3.0 21 1.0 22 3.0 23 4.0 24 5.0 25 4.0 26 3.0 27 6.0 28 12.0 29 17.0 30 19.0 31 32.0 32 56.0 33 78.0 34 213.0 35 507.0 36 2776.0 37 247.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.0802407221665 19.608826479438317 14.167502507522567 31.14343029087262 2 26.525 26.375 31.025000000000002 16.075 3 21.925 27.250000000000004 30.475 20.349999999999998 4 22.400000000000002 33.650000000000006 24.9 19.05 5 26.575 34.975 21.875 16.575 6 22.45 39.300000000000004 22.2 16.05 7 18.625 22.975 38.625 19.775000000000002 8 19.650000000000002 26.625 30.049999999999997 23.674999999999997 9 22.7 25.35 29.25 22.7 10-14 22.845 28.935 27.025 21.195 15-19 23.395 27.725 28.57 20.31 20-24 22.501750525157547 29.17875362608783 28.00340102030609 20.316094828448534 25-29 23.006503251625812 28.319159579789893 28.129064532266135 20.54527263631816 30-34 22.03771319961987 27.429600360126045 29.340269094182965 21.192417346071124 35-39 22.64179253776133 27.928378513554065 28.123437031109333 21.306391917575272 40-44 22.29780423148102 28.10983844345521 28.815085279847946 20.777272045215824 45-49 22.676803040912276 29.01370411123337 27.378213464039213 20.931279383815145 50-54 23.520880220055012 28.11702925731433 28.537134283570893 19.824956239059766 55-59 22.81640820410205 27.54377188594297 28.629314657328663 21.010505252626313 60-64 22.81184355306592 28.05841752525758 28.303491047314193 20.82624787436231 65-69 22.64066016504126 27.406851712928233 28.66216554138535 21.29032258064516 70-74 22.59629814907454 27.668834417208604 28.159079539769884 21.575787893946973 75-79 22.63405362144858 27.44097639055622 28.156262505002 21.768707482993197 80-84 23.470867716929234 27.711927981995498 28.077019254813703 20.740185046261566 85-89 23.78070131559202 28.04762142964334 28.152668700915413 20.019008553849233 90-94 23.116935080524158 28.133440032009606 28.093428028408525 20.65619685905772 95-99 23.82595648912228 28.247061765441362 27.561890472618156 20.365091272818205 100-104 23.696848424212106 27.70885442721361 27.27863931965983 21.315657828914457 105-109 23.240458206192788 28.75293882247011 27.692461607723473 20.314141363613626 110-114 23.572071621486444 28.863659097729315 27.01810543162949 20.54616384915475 115-119 23.3343337334934 28.851540616246496 27.751100440176067 20.063025210084035 120-124 24.452226113056525 28.684342171085543 27.303651825912954 19.559779889944974 125-129 23.70829790426649 27.864752663432203 27.81973690791777 20.607212524383534 130-134 24.837386170319224 28.810167116981887 26.338436905834083 20.014009806864806 135-139 23.938165991295214 27.865325929261093 27.75026264445445 20.446245434989244 140-144 25.44263278983695 27.983395018505554 27.02310693207962 19.550865259577872 145-149 25.767883941970986 28.51425712856428 25.887943971985994 19.82991495747874 150-151 25.59739772300763 29.51332415863881 25.43475541098461 19.454522707368948 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.5 14 1.0 15 1.0 16 1.0 17 1.5 18 1.5 19 0.5 20 1.0 21 3.0 22 2.5 23 1.5 24 3.0 25 3.5 26 7.0 27 12.5 28 13.0 29 11.5 30 18.5 31 30.5 32 37.5 33 40.5 34 48.0 35 67.5 36 91.0 37 107.0 38 133.0 39 170.0 40 205.0 41 226.0 42 240.0 43 281.5 44 295.5 45 282.0 46 247.0 47 216.0 48 213.0 49 195.5 50 159.5 51 131.0 52 112.0 53 96.5 54 85.5 55 53.5 56 35.0 57 33.5 58 23.0 59 18.0 60 13.5 61 5.0 62 4.5 63 3.0 64 3.0 65 3.5 66 2.0 67 0.5 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 1.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.03 25-29 0.05 30-34 0.034999999999999996 35-39 0.03 40-44 0.034999999999999996 45-49 0.03 50-54 0.025 55-59 0.05 60-64 0.03 65-69 0.025 70-74 0.05 75-79 0.04 80-84 0.025 85-89 0.045 90-94 0.03 95-99 0.025 100-104 0.05 105-109 0.045 110-114 0.03 115-119 0.04 120-124 0.05 125-129 0.034999999999999996 130-134 0.06999999999999999 135-139 0.055 140-144 0.03 145-149 0.05 150-151 0.08750000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.625 #Duplication Level Percentage of deduplicated Percentage of total 1 70.30965391621129 48.25 2 19.12568306010929 26.25 3 6.885245901639345 14.174999999999999 4 2.3679417122040074 6.5 5 0.9471766848816029 3.25 6 0.29143897996357016 1.2 7 0.03642987249544627 0.17500000000000002 8 0.03642987249544627 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAGCTGCCAGCCCTCAGGCTAGTGCCTAAGGCCTATATCAAGAAAGGATT 8 0.2 No Hit TTTCTTCTCTCAAGTCTTTAAATTTATCGGGCAATATGTTGCTAGGATCG 7 0.17500000000000002 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 6 0.15 No Hit TTTAGATTTGCTAGAGAGGCTATAATTTCTCAGGTCACGATGCAGCCAGC 6 0.15 No Hit GAGGAATCAACTTTCACCCAGAGGTTCCGAACTGACGAAGTGCATGCAAT 6 0.15 No Hit AATTGAGTCCAATATGTTGACGAAATCACTAGATGAAGCTCAGAGGAAAG 6 0.15 No Hit CAATAGATATCCTGTAGAGATAGGAAAACTAGTTCGGCTTTTGTTGGCTG 6 0.15 No Hit GCAGGACCTGGTCACAGTATTGCTGTGACATCGAATGGAGTAGCTTACTC 6 0.15 No Hit CACTGCTTTGGAGATATTTCTTCTTGGAGCTTTAGCAAAGCTTGGAGCTA 6 0.15 No Hit CCCAAAGAGAAGTTTGCTAAATACAGTCATTCCAGGATGATTATGAAGTG 6 0.15 No Hit CTGCATAGCTTATAAGCTTGTAAGAGATGGCTTCCTCCTCTATGATCTCA 5 0.125 No Hit GCCAAGGACCGAACCAAGTATCAATGTTTGATACCTAGTCTTTTTTTCCT 5 0.125 No Hit GTAACATGGTGAGAAGAATCTACATATGCTCAAGTCAGGATGTGAAAAGG 5 0.125 No Hit ATTAGACAAGTTAGAGTCGCGGAGGATTATAATGCAAGAATTTGTTAATC 5 0.125 No Hit TGAGCACGAGTTTTGTTTATTTAGATATAGCTTGTCAATCCATTACTGCG 5 0.125 No Hit GTAGGAATTGTGTGGTTTCTGATGGCCATGGATGATCAGAAGGCATTGGC 5 0.125 No Hit AAGCAGAAAACAAAGCTCACAAAAAAATCTCTTCCATTGCATCATGGGAG 5 0.125 No Hit TGTCCATCAACTGATAGCACTTTTGATCTTCGGACTGGAGCCATCAAAGA 5 0.125 No Hit GTGGTGCAAATTTTATTGCCTCCAATGCAGATACTGTTGCCCCTAACACT 5 0.125 No Hit CGACGAGCTTAACCGCTCGAATGTCCCCAACAATACCACACCCCCACCAT 5 0.125 No Hit AAGAGGTTGTCAAGGCTGCACAAGGACGAGTTCCGGTGTTCTTGGACGGT 5 0.125 No Hit AAGGCGATGACAAGGTAGTTGATGAGGAGAAGCTTAAGCCAAGAAAGATA 5 0.125 No Hit GCAGGGTTGCCAAACACATTGAACGGGGCTAAAAGAGAAGATCATAGGAA 5 0.125 No Hit GCTCCTCTTATCCGTGATGGTCGTATGGAGAAATTCTACTGGGCTCCAAC 5 0.125 No Hit TAATTTTACTATTGCAATCACTTGGCAACCTGAGATTCTCTTCTCTTTCA 5 0.125 No Hit CCAAGGTTTCAGCCTCTCCACTTCCAAGGCTCAGCATCAAGGCTTCATTG 5 0.125 No Hit CTTCTTATGATTAGGCCCCTACCTTCTATTCTTTTCTCATATCCCTCAGG 5 0.125 No Hit CAAACGAACAACAGGAGATCTATTTGCAATAAAGGTGCTCAAGAAATTGG 5 0.125 No Hit GGGGTCACTTGTTTGAAGGAGGAAACCTCTTTCCTTAGAGACACTTGGGA 5 0.125 No Hit CTTCCGGCGACTTACAAAACCCGATTCATCGAAGCGAACCAAACGAAAAC 5 0.125 No Hit GATAAGTATATGGTGCTGTGAACTCTCAGAGGAGGAGGAGAGATTTCTTC 5 0.125 No Hit ATCTTGGTGTTAGGGCTCGTCAAGAGAGGGTTGTCTTTGTGACATGCGAA 5 0.125 No Hit CTCTGCTCCAAACTTTTCTCTACCCTCGAAACCCTAATCTTCGAGTTCAC 5 0.125 No Hit TCCAGCTTTGGATCATCACACTCCAATTTATGCATCATCCTTTACAATGG 5 0.125 No Hit CCGTTGTCAAAATACAAATCAAGGTTAGTTTCATGTCATTCTTCTTGCCT 5 0.125 No Hit GGTGTTTGAGGACAATGTTTTAATTCGTGAGTTTCTTGAGGAGAAGGGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0125 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.125 0.0 0.0 0.0 0.0 84-85 0.2 0.0 0.0 0.0 0.0 86-87 0.225 0.0 0.0 0.0 0.0 88-89 0.25 0.0 0.0 0.0 0.0 90-91 0.3875 0.0 0.0 0.0 0.0 92-93 0.5375000000000001 0.0 0.0 0.0 0.0 94-95 0.6875 0.0 0.0 0.0 0.0 96-97 0.8625 0.0 0.0 0.0 0.0 98-99 1.1125 0.0 0.0 0.0 0.0 100-101 1.4625 0.0 0.0 0.0 0.0 102-103 1.5625 0.0 0.0 0.0 0.0 104-105 1.775 0.0 0.0 0.0 0.0 106-107 2.0875 0.0 0.0 0.0 0.0 108-109 2.4 0.0 0.0 0.0 0.0 110-111 2.5625 0.0 0.0 0.0 0.0 112-113 2.8125 0.0 0.0 0.0 0.0 114-115 3.0125 0.0 0.0 0.0 0.0 116-117 3.425 0.0 0.0 0.0 0.0 118-119 3.7875 0.0 0.0 0.0 0.0 120-121 3.9625 0.0 0.0 0.0 0.0 122-123 4.3375 0.0 0.0 0.0 0.0 124-125 4.675 0.0 0.0 0.0 0.0 126-127 5.175000000000001 0.0 0.0 0.0 0.0 128-129 5.5 0.0 0.0 0.0 0.0 130-131 5.9 0.0 0.0 0.0 0.0 132-133 6.237500000000001 0.0 0.0 0.0 0.0 134-135 6.7625 0.0 0.0 0.0 0.0 136-137 7.475 0.0 0.0 0.0 0.0 138-139 8.2875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATGAT 10 0.006830828 145.0 6 AAACAGA 10 0.006830828 145.0 145 ATGATCG 10 0.006830828 145.0 8 TGATCGA 10 0.006830828 145.0 9 GCCAGGA 10 0.006830828 145.0 6 GACATGA 10 0.006830828 145.0 5 AGCAGAC 15 1.1411342E-4 145.0 1 CAGGATG 10 0.006830828 145.0 8 CCAGGAT 10 0.006830828 145.0 7 >>END_MODULE Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713533 spots for SRR13695489.sra Written 713533 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra Read 713515 spots for SRR13695489.sra Written 713515 spots for SRR13695489.sra SRR ids: ['SRR13695489.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_4vtoe785 SRR13695489.sra spots: 14270318 blocks: [[1, 713515], [713516, 1427030], [1427031, 2140545], [2140546, 2854060], [2854061, 3567575], [3567576, 4281090], [4281091, 4994605], [4994606, 5708120], [5708121, 6421635], [6421636, 7135150], [7135151, 7848665], [7848666, 8562180], [8562181, 9275695], [9275696, 9989210], [9989211, 10702725], [10702726, 11416240], [11416241, 12129755], [12129756, 12843270], [12843271, 13556785], [13556786, 14270318]] SRR13695489 file size 4827978 SRR13695489 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695489 SRR13695489_1.fastq SRR13695489_2.fastq Input file: SRR13695489_1.fastq Paired file: SRR13695489_2.fastq trimmed: SRR13695489-trimmed-pair1.fastq, SRR13695489-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 07:29:05 2025 >> started Wed Feb 12 07:29:20 2025 >> done (14.596s) 14270318 read pairs processed; of these: 79 ( 0.00%) short read pairs filtered out after trimming by size control 730 ( 0.01%) empty read pairs filtered out after trimming by size control 14269509 (99.99%) read pairs available; of these: 1659396 (11.63%) trimmed read pairs available after processing 12610113 (88.37%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 2 0.00% 20 0 0.00% 21 1 0.00% 22 0 0.00% 23 0 0.00% 24 1 0.00% 25 3 0.00% 26 0 0.00% 27 3 0.00% 28 2 0.00% 29 1 0.00% 30 2 0.00% 31 2 0.00% 32 1 0.00% 33 6 0.00% 34 2 0.00% 35 4 0.00% 36 2 0.00% 37 3 0.00% 38 7 0.00% 39 4 0.00% 40 4 0.00% 41 4 0.00% 42 7 0.00% 43 10 0.00% 44 7 0.00% 45 11 0.00% 46 11 0.00% 47 12 0.00% 48 19 0.00% 49 19 0.00% 50 22 0.00% 51 22 0.00% 52 32 0.00% 53 39 0.00% 54 25 0.00% 55 39 0.00% 56 50 0.00% 57 55 0.00% 58 76 0.00% 59 77 0.00% 60 84 0.00% 61 114 0.00% 62 130 0.00% 63 164 0.00% 64 184 0.00% 65 195 0.00% 66 191 0.00% 67 246 0.00% 68 324 0.00% 69 312 0.00% 70 388 0.00% 71 484 0.00% 72 548 0.00% 73 708 0.00% 74 720 0.01% 75 898 0.01% 76 987 0.01% 77 1059 0.01% 78 1210 0.01% 79 1429 0.01% 80 1496 0.01% 81 1880 0.01% 82 2074 0.01% 83 2282 0.02% 84 2701 0.02% 85 3218 0.02% 86 3507 0.02% 87 3799 0.03% 88 4208 0.03% 89 4462 0.03% 90 5114 0.04% 91 5408 0.04% 92 5735 0.04% 93 6667 0.05% 94 7223 0.05% 95 8138 0.06% 96 8249 0.06% 97 9305 0.07% 98 9595 0.07% 99 9942 0.07% 100 10879 0.08% 101 11165 0.08% 102 11816 0.08% 103 12868 0.09% 104 13387 0.09% 105 14211 0.10% 106 15136 0.11% 107 16073 0.11% 108 16600 0.12% 109 17434 0.12% 110 18125 0.13% 111 18487 0.13% 112 19929 0.14% 113 19920 0.14% 114 21148 0.15% 115 22158 0.16% 116 23228 0.16% 117 24124 0.17% 118 25053 0.18% 119 25476 0.18% 120 26479 0.19% 121 27187 0.19% 122 27806 0.19% 123 29268 0.21% 124 29726 0.21% 125 29892 0.21% 126 31331 0.22% 127 32777 0.23% 128 33226 0.23% 129 33792 0.24% 130 34835 0.24% 131 35108 0.25% 132 35343 0.25% 133 36570 0.26% 134 36983 0.26% 135 37487 0.26% 136 38932 0.27% 137 39666 0.28% 138 40886 0.29% 139 42634 0.30% 140 42597 0.30% 141 43600 0.31% 142 43713 0.31% 143 44085 0.31% 144 44976 0.32% 145 46036 0.32% 146 46081 0.32% 147 47256 0.33% 148 49168 0.34% 149 48670 0.34% 150 50104 0.35% 151 12610113 88.37% 14269509 reads passed initial QC criterion=sequence-density sequence-density=0.50 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=21 prefix-density=0.49 prefix-fanout=2.0 sequence=GTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAG criterion=fanout-score sequence-density=0.02 sequence-density-rank=24 fanout-score=317.70 fanout-score-rank=1 prefix-density=0.29 prefix-fanout=17.0 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTC criterion=sequence-density sequence-density=1.15 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=30 prefix-density=1.14 prefix-fanout=2.0 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGAC criterion=fanout-score sequence-density=0.18 sequence-density-rank=23 fanout-score=9.84 fanout-score-rank=1 prefix-density=1.17 prefix-fanout=1.5 sequence=TCAATCAATCACCATGTCTAGCA SRR13695489 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 07:30:01 Started mapping on | Feb 12 07:30:01 Finished on | Feb 12 07:31:28 Mapping speed, Million of reads per hour | 590.46 Number of input reads | 14269509 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 13540213 Uniquely mapped reads % | 94.89% Average mapped length | 295.41 Number of splices: Total | 13593079 Number of splices: Annotated (sjdb) | 13296182 Number of splices: GT/AG | 13302642 Number of splices: GC/AG | 230163 Number of splices: AT/AC | 7854 Number of splices: Non-canonical | 52420 Mismatch rate per base, % | 0.36% Deletion rate per base | 0.03% Deletion average length | 2.83 Insertion rate per base | 0.02% Insertion average length | 2.34 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 325978 % of reads mapped to multiple loci | 2.28% Number of reads mapped to too many loci | 68664 % of reads mapped to too many loci | 0.48% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.23% % of reads unmapped: other | 0.11% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 403496 403496 403496 N_multimapping 325978 325978 325978 N_noFeature 549998 13157756 781470 N_ambiguous 244441 1767 92042 UnstrandedReadsAssigned:12745774 PositiveStrandReadsAssigned:380690 NegativeStrandReadsAssigned:12666701 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695489 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695489-trimmed-pair1.fastq SRR13695489-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 14,269,509 reads, 12,692,531 reads pseudoaligned [quant] estimated average fragment length: 249.287 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 991 rounds 52401 SRR13695489.ke.tsv 34699 SRR13695489.se.tsv 87100 total ==> SRR13695489.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1769.71 388 14.5186 Potri.005G024800.1.v4.1 1035 786.713 169 14.2255 Potri.004G059700.1.v4.1 961 712.797 0 0 Potri.007G009000.2.v4.1 1416 1167.71 0 0 Potri.003G141000.2.v4.1 2943 2694.71 680.54 16.7239 Potri.016G087400.1.v4.1 270 83.9935 672 529.81 Potri.015G069301.1.v4.1 564 324.661 0 0 Potri.010G195200.1.v4.1 1773 1524.71 58 2.51905 Potri.012G127500.1.v4.1 977 728.756 38 3.45302 ==> SRR13695489.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 65 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 225 Potri.001G212900.v4.1 5 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 3 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695489 completed mapping pipeline successfully