Starting /dee2/code/volunteer_pipeline.sh SRR13695490 current disk space = 3050292740096 free memory = 1018378280 SRR13695490 SRAfilesize f288a588726f66a49e438b481b3cc288 SRR13695490.sra SRR13695490.sra file validated SRR13695490 is paired end SRR13695490 is conventional basespace SRR13695490 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695490_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.591 37.0 37.0 37.0 37.0 37.0 2 36.276 37.0 37.0 37.0 37.0 37.0 3 36.5885 37.0 37.0 37.0 37.0 37.0 4 36.513 37.0 37.0 37.0 37.0 37.0 5 36.604 37.0 37.0 37.0 37.0 37.0 6 36.565 37.0 37.0 37.0 37.0 37.0 7 36.4675 37.0 37.0 37.0 37.0 37.0 8 36.556 37.0 37.0 37.0 37.0 37.0 9 36.533 37.0 37.0 37.0 37.0 37.0 10-14 36.5727 37.0 37.0 37.0 37.0 37.0 15-19 36.5279 37.0 37.0 37.0 37.0 37.0 20-24 36.5647 37.0 37.0 37.0 37.0 37.0 25-29 36.5025 37.0 37.0 37.0 37.0 37.0 30-34 36.462599999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.467699999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.4617 37.0 37.0 37.0 37.0 37.0 45-49 36.451699999999995 37.0 37.0 37.0 37.0 37.0 50-54 36.362100000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.388799999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.3787 37.0 37.0 37.0 37.0 37.0 65-69 36.3084 37.0 37.0 37.0 37.0 37.0 70-74 36.3279 37.0 37.0 37.0 37.0 37.0 75-79 36.3086 37.0 37.0 37.0 37.0 37.0 80-84 36.2659 37.0 37.0 37.0 37.0 37.0 85-89 36.2937 37.0 37.0 37.0 37.0 37.0 90-94 36.2106 37.0 37.0 37.0 37.0 37.0 95-99 36.1659 37.0 37.0 37.0 37.0 37.0 100-104 36.165 37.0 37.0 37.0 37.0 37.0 105-109 36.163599999999995 37.0 37.0 37.0 37.0 37.0 110-114 36.131 37.0 37.0 37.0 37.0 37.0 115-119 36.0795 37.0 37.0 37.0 37.0 37.0 120-124 35.9882 37.0 37.0 37.0 37.0 37.0 125-129 35.9681 37.0 37.0 37.0 37.0 37.0 130-134 36.057599999999994 37.0 37.0 37.0 37.0 37.0 135-139 35.95649999999999 37.0 37.0 37.0 37.0 37.0 140-144 35.7995 37.0 37.0 37.0 37.0 37.0 145-149 35.7205 37.0 37.0 37.0 37.0 37.0 150-151 35.57725 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 4.0 24 1.0 25 2.0 26 1.0 27 11.0 28 14.0 29 19.0 30 22.0 31 30.0 32 40.0 33 59.0 34 152.0 35 312.0 36 2979.0 37 354.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.55 11.575000000000001 9.125 36.75 2 22.110552763819097 12.21105527638191 35.35175879396985 30.326633165829147 3 18.55 19.6 25.974999999999998 35.875 4 21.975 27.375 23.474999999999998 27.175 5 23.825 30.625000000000004 24.275 21.275 6 21.575 34.375 22.025 22.025 7 16.475 27.474999999999998 40.5 15.55 8 17.150000000000002 26.0 34.525 22.325 9 17.525 23.5 34.8 24.175 10-14 20.355 29.205 27.72 22.720000000000002 15-19 20.294999999999998 28.425 28.34 22.939999999999998 20-24 20.330000000000002 28.125 27.310000000000002 24.235 25-29 21.05 27.415 27.944999999999997 23.59 30-34 20.04 28.595 27.529999999999998 23.835 35-39 20.32 28.525 26.775 24.38 40-44 21.535 28.215 26.815 23.435 45-49 20.77 28.360000000000003 27.525 23.345 50-54 20.53 29.34 26.905 23.225 55-59 20.72 28.605000000000004 27.794999999999998 22.88 60-64 20.905 28.575 26.695 23.825 65-69 20.1 28.065 28.235 23.599999999999998 70-74 21.275 28.62 27.275 22.830000000000002 75-79 20.1 28.125 27.99 23.785 80-84 20.985 27.865000000000002 27.49 23.66 85-89 20.47 28.235 28.035 23.26 90-94 20.599999999999998 27.455000000000002 27.815 24.13 95-99 21.07 29.020000000000003 26.845000000000002 23.064999999999998 100-104 20.495 29.110000000000003 27.045 23.35 105-109 20.990000000000002 28.810000000000002 27.229999999999997 22.97 110-114 21.055 28.865000000000002 26.455000000000002 23.625 115-119 21.36 27.91 27.134999999999998 23.595 120-124 21.385 27.689999999999998 28.110000000000003 22.814999999999998 125-129 21.64 28.16 27.045 23.155 130-134 20.485 28.78 26.590000000000003 24.145 135-139 21.745 28.794999999999998 27.26 22.2 140-144 21.29 27.779999999999998 27.279999999999998 23.65 145-149 21.67 27.935 26.655 23.74 150-151 21.975 29.037499999999998 26.275 22.7125 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 0.5 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 2.0 19 2.0 20 1.5 21 1.5 22 0.0 23 0.0 24 0.5 25 4.5 26 9.5 27 9.5 28 10.5 29 13.5 30 17.0 31 20.5 32 33.0 33 42.5 34 52.5 35 68.5 36 84.0 37 87.5 38 103.5 39 145.5 40 186.5 41 215.5 42 234.5 43 231.0 44 254.0 45 282.5 46 256.5 47 240.5 48 228.0 49 209.5 50 196.5 51 161.5 52 125.0 53 101.5 54 79.5 55 69.0 56 55.0 57 46.0 58 37.0 59 27.0 60 15.5 61 6.5 62 8.0 63 7.0 64 5.5 65 3.5 66 2.0 67 1.5 68 1.0 69 0.5 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.15 #Duplication Level Percentage of deduplicated Percentage of total 1 70.6529713866471 48.15 2 18.892149669845928 25.75 3 6.272927366104182 12.825000000000001 4 2.5678650036683783 7.000000000000001 5 0.8437270726338958 2.875 6 0.5135730007336757 2.1 7 0.1467351430667645 0.7000000000000001 8 0.11005135730007337 0.6 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGACAATCTCGTTGGTAAATGCTACATCCACTGCCAGTTCGTGGGGCATA 8 0.2 No Hit GCTTCCACATAGTCCAGTAGCGTCCATCATAGTACCCTGGTGAGCTGTGG 8 0.2 No Hit GCGGGCGGCATAGGTTATTGTGGAGCAGAGGAGGAGGGCTACCAAGAAGA 8 0.2 No Hit GGAGTCATACTTGAGAAGGTGGGAGGCCTGCTTAACACCACCAGTGTCGT 7 0.17500000000000002 No Hit GTTAGCCTTTCTGGTGACCGGGAAAGCTGAGGTAGACTTGAGGCCGTTGA 7 0.17500000000000002 No Hit CCAGAGAGGAAATCACCCAAGTCTACAAGTGGGACTTGGAGTTCAGGTGC 7 0.17500000000000002 No Hit GCAATATGTACTGCTATAAGGAAAATTCATGAACATCGAAGGAGACGGGC 7 0.17500000000000002 No Hit CAGCACCATTTCCTTTGGGGTTATTGGGATCGACACCAGGCCATTCACGG 6 0.15 No Hit TTCTCATTGTGCACAGAATTTTTGTAGGCGATTTGAGAATTACAAAGGGA 6 0.15 No Hit CCACCATTCATCCCAGAACCATAAAGCCCACCATAACCGCCTCGATACAT 6 0.15 No Hit GTGAGCTTGTTGTTTGGGAAGATAGGATCAGTGTTAACACACCCTGGCTT 6 0.15 No Hit TATACATTGTGCCAAGCTGCTTGACACCTGGCCACATGAGATGGCAGCTT 6 0.15 No Hit ATCTAATTCTATCTTCAAGTTGTTGGGAAGCCGTGATGAAGAATTACATC 6 0.15 No Hit CGTTTCGTAACGCACTTCACCAGTGCAATAATCATAGAGCTCGTACTGAA 6 0.15 No Hit GTGGTGCTCACGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAG 6 0.15 No Hit CTTCTGCACCCTGGTTACAGGCATAAACTAAAGCATCTGCAAGTGTAAGC 6 0.15 No Hit CAACAAGGAGCACACAGGCTGCCAGAGCAAAATGTAAAAGAGTAAATTCA 6 0.15 No Hit GCATCTAAGAGCAAAGAAGGCAAATCCGGAGGAGCAGATTCAGGTCTTCC 6 0.15 No Hit CACAGGAGAAGAGATCAACACCGTGGCGACGGAAGAACTGCTTGGAGAGT 6 0.15 No Hit CTTGTATTGAGAAAATCAAAAGCTTGCTGTACAGTTTCAATGGATGCAAA 6 0.15 No Hit TGTGGGATGACGAAAATATGTGAATAACTAGCACCAAAACGCTTTATCTT 6 0.15 No Hit TGAGCAGAGAGTGTGAAAAAGGGCAGCAGCTGCATTAGTCCTCGTCTCAA 5 0.125 No Hit CCAGTCTGAATTAAATCTTCCATGGCTATTCCCATTCCCCTGTAGTTTCT 5 0.125 No Hit CACTGCATTCTTTATTACAATGCTCATGGTATACACAGATGGCATATGCT 5 0.125 No Hit TGCTCCTTTCCTCATCTTTTCCCATGCTTGTAGTGTTTCCTCGGCAAGCA 5 0.125 No Hit GTGAAATTTTGGAGTTTGTGAACCTTGATGAGCTCACTTTCTTCAAGCTG 5 0.125 No Hit GTACCATACAGATTATGCACTTTCAGCACATATGATTAACATCATGGTCT 5 0.125 No Hit CTCTTTGACAGCTGTTTCCTCTGCAGCAGCAACCTTCATGGTTCTTTGTG 5 0.125 No Hit GACCTCTTTTCCTTCTTCTTCTTCTTTTTCTCTTCATCGTCACCTTCCTC 5 0.125 No Hit GCATCTTTCAGCAAATAATTTTGAAATGCTTTCCAGTGTCCACCTTCCTC 5 0.125 No Hit GTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGATGCCTTATGT 5 0.125 No Hit CCAGCATCCAATCACCATCCTTGTCTTCAAATGTGAGAACATATTCAGAA 5 0.125 No Hit CCGGTCTGCCTCCCCATGTCTTGAGGCAAGTGTTTTGAAGAGCTCTTGCA 5 0.125 No Hit CCCTGAATCTATGATAGCACCAGCAGTTGAGAAAACTGATGCAGAGATGG 5 0.125 No Hit AGCATCCGAATCCAGAAAGCAAAAACAAAGTAGAATATTGATCTTCAAAA 5 0.125 No Hit GGGACATCAAATAAATAGTCTATTAGCTTTCAAAACAAGAAATATATAAA 5 0.125 No Hit GGACGGCAGACTCTTTAAATGTCCACTGCACTGCTTATGGTCCGAAGAAT 5 0.125 No Hit TTCCAACATCCATAAGATATAATTGGGTGCTATTCTTACAGGTTTCGTGA 5 0.125 No Hit CCCAAATTTGCTTTATTAGATCCTCTCGCATTCGCATTGCCAAAGGATCT 5 0.125 No Hit AGGCCACTTCTTGCTATGCCACCACCTTCATCTGAGACCTTTATAGTGAC 5 0.125 No Hit GCAAGGGTACGGCCATCCTCAAGCTGCTTGCCGGCAAAGATGAGTCTCTG 5 0.125 No Hit GCCATATCATATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATG 5 0.125 No Hit CAGAGACAGAATCAGAGGAACTGCCAAAACTGTAACACTACCTGCTATAA 5 0.125 No Hit CACAAAAATCAGAAATTACATTGCAGGAGTAGAGAGACATCTACTTCCAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.037500000000000006 0.0 0.0 0.0 0.0 82-83 0.0625 0.0 0.0 0.0 0.0 84-85 0.1 0.0 0.0 0.0 0.0 86-87 0.15 0.0 0.0 0.0 0.0 88-89 0.16249999999999998 0.0 0.0 0.0 0.0 90-91 0.1875 0.0 0.0 0.0 0.0 92-93 0.21250000000000002 0.0 0.0 0.0 0.0 94-95 0.375 0.0 0.0 0.0 0.0 96-97 0.5625 0.0 0.0 0.0 0.0 98-99 0.95 0.0 0.0 0.0 0.0 100-101 1.0625 0.0 0.0 0.0 0.0 102-103 1.1875 0.0 0.0 0.0 0.0 104-105 1.3125 0.0 0.0 0.0 0.0 106-107 1.5750000000000002 0.0 0.0 0.0 0.0 108-109 1.7125 0.0 0.0 0.0 0.0 110-111 1.75 0.0 0.0 0.0 0.0 112-113 2.0 0.0 0.0 0.0 0.0 114-115 2.2249999999999996 0.0 0.0 0.0 0.0 116-117 2.5 0.0 0.0 0.0 0.0 118-119 2.7625 0.0 0.0 0.0 0.0 120-121 3.0625 0.0 0.0 0.0 0.0 122-123 3.325 0.0 0.0 0.0 0.0 124-125 3.6375 0.0 0.0 0.0 0.0 126-127 4.2875 0.0 0.0 0.0 0.0 128-129 4.725 0.0 0.0 0.0 0.0 130-131 5.449999999999999 0.0 0.0 0.0 0.0 132-133 5.675 0.0 0.0 0.0 0.0 134-135 5.9875 0.0 0.0 0.0 0.0 136-137 6.5375 0.0 0.0 0.0 0.0 138-139 7.237500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13695490 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695490_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.8985 37.0 37.0 37.0 37.0 37.0 2 36.0065 37.0 37.0 37.0 37.0 37.0 3 36.143 37.0 37.0 37.0 37.0 37.0 4 36.171 37.0 37.0 37.0 37.0 37.0 5 36.2475 37.0 37.0 37.0 37.0 37.0 6 36.182 37.0 37.0 37.0 37.0 37.0 7 36.2395 37.0 37.0 37.0 37.0 37.0 8 36.3055 37.0 37.0 37.0 37.0 37.0 9 36.2895 37.0 37.0 37.0 37.0 37.0 10-14 36.280699999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.1848 37.0 37.0 37.0 37.0 37.0 20-24 36.1688 37.0 37.0 37.0 37.0 37.0 25-29 36.11185 37.0 37.0 37.0 37.0 37.0 30-34 36.13005 37.0 37.0 37.0 37.0 37.0 35-39 36.04295 37.0 37.0 37.0 37.0 37.0 40-44 36.032149999999994 37.0 37.0 37.0 37.0 37.0 45-49 36.02525000000001 37.0 37.0 37.0 37.0 37.0 50-54 35.98585 37.0 37.0 37.0 37.0 37.0 55-59 36.02525 37.0 37.0 37.0 37.0 37.0 60-64 35.95525 37.0 37.0 37.0 37.0 37.0 65-69 35.85665 37.0 37.0 37.0 37.0 37.0 70-74 35.873450000000005 37.0 37.0 37.0 37.0 37.0 75-79 35.87335 37.0 37.0 37.0 37.0 37.0 80-84 35.91324999999999 37.0 37.0 37.0 37.0 37.0 85-89 35.76905 37.0 37.0 37.0 37.0 37.0 90-94 35.80735 37.0 37.0 37.0 37.0 37.0 95-99 35.79075 37.0 37.0 37.0 37.0 37.0 100-104 35.754949999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.711149999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.65065 37.0 37.0 37.0 37.0 37.0 115-119 35.64255 37.0 37.0 37.0 37.0 37.0 120-124 35.606849999999994 37.0 37.0 37.0 37.0 37.0 125-129 35.56695 37.0 37.0 37.0 37.0 37.0 130-134 35.50095 37.0 37.0 37.0 37.0 37.0 135-139 35.52045 37.0 37.0 37.0 37.0 37.0 140-144 35.468450000000004 37.0 37.0 37.0 37.0 37.0 145-149 35.295550000000006 37.0 37.0 37.0 29.8 37.0 150-151 35.08175 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 3.0 15 1.0 16 2.0 17 0.0 18 1.0 19 2.0 20 3.0 21 1.0 22 6.0 23 4.0 24 3.0 25 10.0 26 5.0 27 10.0 28 13.0 29 18.0 30 29.0 31 44.0 32 49.0 33 102.0 34 197.0 35 632.0 36 2692.0 37 169.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.048144433299896 20.386158475426278 12.462387161484454 26.10330992978937 2 27.700000000000003 24.425 30.599999999999998 17.275 3 20.95 25.6 34.075 19.375 4 24.5 34.9 21.325 19.275000000000002 5 24.675 36.875 21.925 16.525000000000002 6 20.200000000000003 40.550000000000004 21.575 17.675 7 20.075000000000003 22.425 39.050000000000004 18.45 8 20.849999999999998 25.825 28.1 25.224999999999998 9 20.65 24.7 32.275 22.375 10-14 21.77 29.875 27.82 20.535 15-19 22.25 28.499999999999996 27.88 21.37 20-24 22.911037726408487 29.975983188231766 26.62363654558191 20.489342539777844 25-29 22.912184138103576 28.126094570928196 27.910933199899922 21.050788091068302 30-34 22.13660245183888 28.526394796097073 27.63572679509632 21.701275956967724 35-39 23.067300475356518 28.171128346259692 27.75081310983237 21.010758068551414 40-44 23.677758318739052 27.68076057042782 28.17613209907431 20.465349011758818 45-49 22.34175631723793 28.211158368776584 28.29622216662497 21.15086314736052 50-54 22.601951463597697 28.006004503377536 28.76157117838379 20.63047285464098 55-59 23.2424318238679 27.705779334500875 28.04103077307981 21.010758068551414 60-64 23.167375531648737 27.105328996747563 28.73154866149612 20.99574681010758 65-69 23.52558651393127 27.172227502376067 28.06763043369516 21.2345555499975 70-74 22.847135351513636 28.241180885664246 27.905929447085313 21.0057543157368 75-79 23.157368026019515 27.35551663747811 27.965974480860645 21.521140855641733 80-84 22.80210157618214 27.440580435326495 28.271203402551915 21.486114585939458 85-89 23.01726294721041 27.87090317738304 28.2661996497373 20.84563422566925 90-94 23.062296722541905 28.066049537152864 27.37553164873655 21.496122091568676 95-99 22.286715036277208 28.841631223417565 27.380535401551164 21.491118338754063 100-104 23.2874655991994 27.675756817613212 27.975981986489867 21.060795596697524 105-109 23.842882161621215 28.256192144108084 27.835876907680763 20.06504878658994 110-114 23.842882161621215 27.980985739304476 27.60570427820866 20.57042782086565 115-119 23.882912184138103 28.071053289967473 27.235426569927444 20.810607955966976 120-124 23.782837127845884 27.820865649236925 28.126094570928196 20.270202651988992 125-129 24.078058543907932 28.116087065298974 26.95021265949462 20.855641731298473 130-134 24.75356517388041 28.08606454841131 26.925193895421568 20.235176382286717 135-139 23.897923442581938 28.531398548911685 27.175381536152116 20.395296472354264 140-144 25.243932949712285 26.855141356017015 27.46059544658494 20.440330247685765 145-149 26.289717287965974 27.33049787340505 26.41481110833125 19.96497373029772 150-151 25.956967725794343 27.683262446835126 25.55666750062547 20.80310232674506 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.5 5 0.5 6 0.5 7 0.5 8 0.5 9 1.0 10 3.5 11 3.5 12 0.5 13 0.0 14 1.5 15 1.5 16 0.0 17 0.0 18 0.0 19 0.5 20 3.5 21 3.0 22 0.5 23 2.5 24 4.5 25 6.0 26 5.5 27 6.5 28 9.5 29 10.0 30 21.0 31 33.0 32 45.0 33 54.0 34 54.5 35 63.5 36 81.0 37 106.5 38 127.0 39 159.0 40 201.0 41 215.5 42 234.5 43 261.5 44 272.5 45 267.5 46 260.0 47 238.5 48 210.5 49 199.0 50 171.0 51 123.5 52 91.5 53 91.5 54 93.0 55 63.0 56 47.5 57 45.5 58 28.0 59 17.5 60 13.5 61 13.5 62 9.0 63 6.5 64 4.0 65 1.5 66 1.0 67 0.5 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.06999999999999999 25-29 0.075 30-34 0.075 35-39 0.075 40-44 0.075 45-49 0.075 50-54 0.075 55-59 0.075 60-64 0.075 65-69 0.045 70-74 0.075 75-79 0.075 80-84 0.075 85-89 0.075 90-94 0.075 95-99 0.075 100-104 0.075 105-109 0.075 110-114 0.075 115-119 0.075 120-124 0.075 125-129 0.075 130-134 0.075 135-139 0.075 140-144 0.075 145-149 0.075 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.30000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 70.68081991215227 48.275 2 18.960468521229867 25.900000000000002 3 6.332357247437774 12.975 4 2.452415812591508 6.7 5 0.8418740849194729 2.875 6 0.4758418740849195 1.95 7 0.18301610541727673 0.8750000000000001 8 0.036603221083455345 0.2 9 0.0 0.0 >10 0.036603221083455345 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGCATAGCTTATAAGCTTGTAAGAGATGGCTTCCTCCTCTATGATCTCA 10 0.25 No Hit CAGAATCTTATCAACATCTTATATCCATTCCCGCATAATAATTTTCTTTT 8 0.2 No Hit TGCATAGCTTATAAGCTTGTAAGAGATGGCTTCCTCCTCTATGATCTCAT 7 0.17500000000000002 No Hit ATGCCATCCATAACCACCCCTCATCACCACCCAAAAGATCAGGATCAATG 7 0.17500000000000002 No Hit ACCACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCC 7 0.17500000000000002 No Hit GCTAAGTGAAAGAATCAATGACTGTTACAGATTAGCAGCTCTTAAAACTC 7 0.17500000000000002 No Hit AGGATCGGCAGGAACTTCTTGAGGTGCTGGCATGGACGCAAGGATTCCCC 7 0.17500000000000002 No Hit GTCAGGGAGTGTCACAGAGCAAAGCATCTCATCTAGAAAGAACTATCTGG 6 0.15 No Hit CCTCGAATCTCCAATTCCTCGTGGCCATGGCAGCTCAAGCCTCTCTCTTT 6 0.15 No Hit CCGAAAGATGTGTTACAGGGAGAGATGCTCAACGTGCGGCAAGACCACCT 6 0.15 No Hit GATTAGAAATTCATAGACTTTATATGCATGGACGGAACAAATTGATGGGA 6 0.15 No Hit GGAAAATTATTGATCACCATCCATATCTAAGGAGCATGTAGTTCATCATC 6 0.15 No Hit AGTAGCTGGTGCCTTGGTCTTACCAATAACTGATGCCTTGTCTCTTCAAC 6 0.15 No Hit CTATAACTTATCTACGTTTTTCCCAAATGGTGAAAGGGATGGGAGGACAG 6 0.15 No Hit GGTTTACAATAGGCAGCGTCAGGCCAAGATTACTGGAGAAATATTGGAGA 6 0.15 No Hit CAACCAAGGACATGGAGAAGGTCTTAGATGTTTCAATCAGCCGAATAGCC 6 0.15 No Hit TTCAAGGGCAGTAGTCTTAAACCATACTCTAAAATCTTCTTATAATTCCA 6 0.15 No Hit AGTATGAAAGCTTTAGTGATAGCTATTCTTATAGCTATCATTGCCTTCTC 6 0.15 No Hit CTGGTATGAGGCCAGGAGATATAGTTACAGCCTCAAATGGAAAGACGATT 6 0.15 No Hit GTCATGTAGCTTCACCGAGAAGTGGGTTGCTAATGCAAAAGTTGCAAAGT 6 0.15 No Hit GAGAATCAGAACAACTTTAGAGGAAGAAACCGGACAAGTAGTCAGTTTAA 5 0.125 No Hit GGAAGAACGACTTGAAGCTGAACAAAAGAGACTGGAAAGGATAGGTCCAA 5 0.125 No Hit CTCTGCTTTACTTTCCATCCAATCATCCTAAAACAAACAATATCAGTATA 5 0.125 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 5 0.125 No Hit CATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTG 5 0.125 No Hit GTCATTTATGTAGGCTCCTTGACCAGGTTAAAACTGTATCTTCAAGTCAT 5 0.125 No Hit CCTTGATAGAGTAAGAGAAAAGCAGAGCAAGCGACTTAGAGGCAGCATTA 5 0.125 No Hit CACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCCTC 5 0.125 No Hit GGTAGCACAAAATGCCAGTGATGATGCCCGTGCAATATGTTTACGAGAGT 5 0.125 No Hit GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG 5 0.125 No Hit CCAGAAGGAATCCACACTCCACTTGGTGCTCCGTTTGAGGGGAGGCATGC 5 0.125 No Hit CACACCAGAGCAGGTTGCTGAATACACTCTCAAGCTTCTCCAACGAAGAG 5 0.125 No Hit AATCAGGATATGGTTACCTGTATGTAAAGGTTAGCATGGATGGTGCTCCA 5 0.125 No Hit TGGGGAAGAAAGAAGAAGAGAAGCCTCAAGAGGAGGTGATTGGTACTGAA 5 0.125 No Hit GTGCATCAGTTTTATCGCCTCCAAGCCGAAGGGTGTCTAGGTTCCAAGAT 5 0.125 No Hit AGTAGATTCATCACTAACAATGGTGATGGGAGATGACATGGTTAAGGTGA 5 0.125 No Hit GACCAGTGTTGCTAGAGAAATCAGGTCTCGGAATTTAAAGAGAGAATCCC 5 0.125 No Hit CCAGTATTTATCACCTTATGAGAGGCTATAGCTGCTGCTTCTTGTAGTCA 5 0.125 No Hit AGAAAACCAATTATTCGCATCGGAAAAAGGGAATTTGTTGATGCAGATGA 5 0.125 No Hit GAGAAGAAGAAAGGAGACAAGTCAAGGGTAAAGAGAGTATGAAAGCAGAG 5 0.125 No Hit AATACAACAAGATCTTCTCCTATTGTCTCCCGTCTTCCTCTAGCTCAACC 5 0.125 No Hit TGAAATTTTATCAGACACAGCTCCGAATGTGAAGCACAGGAACTCTCTGA 5 0.125 No Hit AATATCTGAGGTTGACTCCTTCAATGGCTTCAAAGCCTCAAAGCTCCTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.037500000000000006 0.0 0.0 0.0 0.0 82-83 0.0625 0.0 0.0 0.0 0.0 84-85 0.1 0.0 0.0 0.0 0.0 86-87 0.15 0.0 0.0 0.0 0.0 88-89 0.16249999999999998 0.0 0.0 0.0 0.0 90-91 0.1875 0.0 0.0 0.0 0.0 92-93 0.21250000000000002 0.0 0.0 0.0 0.0 94-95 0.375 0.0 0.0 0.0 0.0 96-97 0.5375 0.0 0.0 0.0 0.0 98-99 0.925 0.0 0.0 0.0 0.0 100-101 1.0375 0.0 0.0 0.0 0.0 102-103 1.1625 0.0 0.0 0.0 0.0 104-105 1.2875 0.0 0.0 0.0 0.0 106-107 1.5625 0.0 0.0 0.0 0.0 108-109 1.725 0.0 0.0 0.0 0.0 110-111 1.775 0.0 0.0 0.0 0.0 112-113 2.0250000000000004 0.0 0.0 0.0 0.0 114-115 2.2625 0.0 0.0 0.0 0.0 116-117 2.55 0.0 0.0 0.0 0.0 118-119 2.8125 0.0 0.0 0.0 0.0 120-121 3.1375 0.0 0.0 0.0 0.0 122-123 3.4000000000000004 0.0 0.0 0.0 0.0 124-125 3.7125000000000004 0.0 0.0 0.0 0.0 126-127 4.4 0.0 0.0 0.0 0.0 128-129 4.85 0.0 0.0 0.0 0.0 130-131 5.574999999999999 0.0 0.0 0.0 0.0 132-133 5.8 0.0 0.0 0.0 0.0 134-135 6.1125 0.0 0.0 0.0 0.0 136-137 6.6625 0.0 0.0 0.0 0.0 138-139 7.362500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTCCTT 10 0.006830828 145.0 9 >>END_MODULE Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981238 spots for SRR13695490.sra Written 981238 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra Read 981235 spots for SRR13695490.sra Written 981235 spots for SRR13695490.sra SRR ids: ['SRR13695490.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_174ahwnn SRR13695490.sra spots: 19624703 blocks: [[1, 981235], [981236, 1962470], [1962471, 2943705], [2943706, 3924940], [3924941, 4906175], [4906176, 5887410], [5887411, 6868645], [6868646, 7849880], [7849881, 8831115], [8831116, 9812350], [9812351, 10793585], [10793586, 11774820], [11774821, 12756055], [12756056, 13737290], [13737291, 14718525], [14718526, 15699760], [15699761, 16680995], [16680996, 17662230], [17662231, 18643465], [18643466, 19624703]] SRR13695490 file size 6647632 SRR13695490 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695490 SRR13695490_1.fastq SRR13695490_2.fastq Input file: SRR13695490_1.fastq Paired file: SRR13695490_2.fastq trimmed: SRR13695490-trimmed-pair1.fastq, SRR13695490-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 06:55:13 2025 >> started Wed Feb 12 06:55:35 2025 >> done (22.427s) 19624703 read pairs processed; of these: 136 ( 0.00%) short read pairs filtered out after trimming by size control 1553 ( 0.01%) empty read pairs filtered out after trimming by size control 19623014 (99.99%) read pairs available; of these: 2217003 (11.30%) trimmed read pairs available after processing 17406011 (88.70%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 1 0.00% 20 1 0.00% 21 5 0.00% 22 1 0.00% 23 1 0.00% 24 0 0.00% 25 2 0.00% 26 0 0.00% 27 3 0.00% 28 4 0.00% 29 3 0.00% 30 1 0.00% 31 3 0.00% 32 1 0.00% 33 6 0.00% 34 3 0.00% 35 1 0.00% 36 6 0.00% 37 5 0.00% 38 7 0.00% 39 7 0.00% 40 5 0.00% 41 6 0.00% 42 10 0.00% 43 14 0.00% 44 7 0.00% 45 13 0.00% 46 8 0.00% 47 13 0.00% 48 22 0.00% 49 24 0.00% 50 29 0.00% 51 22 0.00% 52 45 0.00% 53 52 0.00% 54 63 0.00% 55 68 0.00% 56 70 0.00% 57 73 0.00% 58 116 0.00% 59 111 0.00% 60 173 0.00% 61 159 0.00% 62 221 0.00% 63 267 0.00% 64 277 0.00% 65 293 0.00% 66 343 0.00% 67 343 0.00% 68 384 0.00% 69 528 0.00% 70 658 0.00% 71 746 0.00% 72 891 0.00% 73 1031 0.01% 74 1254 0.01% 75 1378 0.01% 76 1611 0.01% 77 1676 0.01% 78 1886 0.01% 79 2247 0.01% 80 2418 0.01% 81 2891 0.01% 82 3306 0.02% 83 3775 0.02% 84 4295 0.02% 85 4860 0.02% 86 5007 0.03% 87 5583 0.03% 88 6184 0.03% 89 6527 0.03% 90 7135 0.04% 91 7964 0.04% 92 8713 0.04% 93 9571 0.05% 94 10365 0.05% 95 11276 0.06% 96 12160 0.06% 97 12650 0.06% 98 13308 0.07% 99 14283 0.07% 100 15034 0.08% 101 15471 0.08% 102 17114 0.09% 103 17477 0.09% 104 18670 0.10% 105 19707 0.10% 106 20757 0.11% 107 22164 0.11% 108 22836 0.12% 109 23690 0.12% 110 24341 0.12% 111 24976 0.13% 112 26469 0.13% 113 26895 0.14% 114 28308 0.14% 115 29727 0.15% 116 31241 0.16% 117 32325 0.16% 118 33158 0.17% 119 33983 0.17% 120 35455 0.18% 121 35940 0.18% 122 36972 0.19% 123 38539 0.20% 124 39445 0.20% 125 39818 0.20% 126 42388 0.22% 127 42821 0.22% 128 43929 0.22% 129 44827 0.23% 130 46048 0.23% 131 46507 0.24% 132 47039 0.24% 133 48196 0.25% 134 49188 0.25% 135 50622 0.26% 136 51209 0.26% 137 52699 0.27% 138 53470 0.27% 139 55505 0.28% 140 55736 0.28% 141 56621 0.29% 142 57254 0.29% 143 57740 0.29% 144 59156 0.30% 145 60334 0.31% 146 60898 0.31% 147 62876 0.32% 148 63370 0.32% 149 63667 0.32% 150 64920 0.33% 151 17406011 88.70% 19623014 reads passed initial QC criterion=sequence-density sequence-density=0.57 sequence-density-rank=1 fanout-score=2.33 fanout-score-rank=10 prefix-density=0.59 prefix-fanout=2.2 sequence=TTAGCCTTTCTGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=20 fanout-score=490.98 fanout-score-rank=1 prefix-density=0.26 prefix-fanout=17.0 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTC criterion=sequence-density sequence-density=0.98 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=24 prefix-density=0.98 prefix-fanout=2.0 sequence=AACCGCACCCCGGCACA criterion=fanout-score sequence-density=0.18 sequence-density-rank=20 fanout-score=15.80 fanout-score-rank=1 prefix-density=0.64 prefix-fanout=4.4 sequence=AATGGCAGCCTCAGTTATGGCTTCACTGAACCTGAAACCATCTCCATTCACGGTTGAGAAGTCTTCAGTGAGAGGCCTCCCAACTCTTTCAAGGAGATCTTTCAAGATT SRR13695490 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 06:56:19 Started mapping on | Feb 12 06:56:20 Finished on | Feb 12 06:58:43 Mapping speed, Million of reads per hour | 494.01 Number of input reads | 19623014 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 18152469 Uniquely mapped reads % | 92.51% Average mapped length | 295.24 Number of splices: Total | 17917349 Number of splices: Annotated (sjdb) | 17543492 Number of splices: GT/AG | 17541412 Number of splices: GC/AG | 310719 Number of splices: AT/AC | 9841 Number of splices: Non-canonical | 55377 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.92 Insertion rate per base | 0.02% Insertion average length | 2.33 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 434295 % of reads mapped to multiple loci | 2.21% Number of reads mapped to too many loci | 130015 % of reads mapped to too many loci | 0.66% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.44% % of reads unmapped: other | 0.18% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1036503 1036503 1036503 N_multimapping 434295 434295 434295 N_noFeature 742341 17643784 1080756 N_ambiguous 284029 2237 111976 UnstrandedReadsAssigned:17126099 PositiveStrandReadsAssigned:506448 NegativeStrandReadsAssigned:16959737 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695490 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695490-trimmed-pair1.fastq SRR13695490-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,623,014 reads, 17,148,766 reads pseudoaligned [quant] estimated average fragment length: 249.41 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,301 rounds 52401 SRR13695490.ke.tsv 34699 SRR13695490.se.tsv 87100 total ==> SRR13695490.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1769.59 468 14.2193 Potri.005G024800.1.v4.1 1035 786.59 173 11.8251 Potri.004G059700.1.v4.1 961 712.709 4 0.301754 Potri.007G009000.2.v4.1 1416 1167.59 0 0 Potri.003G141000.2.v4.1 2943 2694.59 908 18.1175 Potri.016G087400.1.v4.1 270 84.3275 619.693 395.106 Potri.015G069301.1.v4.1 564 324.958 0 0 Potri.010G195200.1.v4.1 1773 1524.59 85 2.99759 Potri.012G127500.1.v4.1 977 728.651 76 5.6079 ==> SRR13695490.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 390 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 309 Potri.001G212900.v4.1 2 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 2 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 7 SRR13695490 completed mapping pipeline successfully