Starting /dee2/code/volunteer_pipeline.sh SRR13695491 current disk space = 3050069471232 free memory = 1307526292 SRR13695491 SRAfilesize 7ced1b08b1b5c16e2840b5bce8325276 SRR13695491.sra SRR13695491.sra file validated SRR13695491 is paired end SRR13695491 is conventional basespace SRR13695491 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695491_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6745 37.0 37.0 37.0 37.0 37.0 2 36.26975 37.0 37.0 37.0 37.0 37.0 3 36.514 37.0 37.0 37.0 37.0 37.0 4 36.6615 37.0 37.0 37.0 37.0 37.0 5 36.616 37.0 37.0 37.0 37.0 37.0 6 36.4855 37.0 37.0 37.0 37.0 37.0 7 36.524 37.0 37.0 37.0 37.0 37.0 8 36.5845 37.0 37.0 37.0 37.0 37.0 9 36.529 37.0 37.0 37.0 37.0 37.0 10-14 36.6128 37.0 37.0 37.0 37.0 37.0 15-19 36.55120000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.5664 37.0 37.0 37.0 37.0 37.0 25-29 36.5458 37.0 37.0 37.0 37.0 37.0 30-34 36.5252 37.0 37.0 37.0 37.0 37.0 35-39 36.4893 37.0 37.0 37.0 37.0 37.0 40-44 36.4771 37.0 37.0 37.0 37.0 37.0 45-49 36.46040000000001 37.0 37.0 37.0 37.0 37.0 50-54 36.4502 37.0 37.0 37.0 37.0 37.0 55-59 36.4311 37.0 37.0 37.0 37.0 37.0 60-64 36.4382 37.0 37.0 37.0 37.0 37.0 65-69 36.3217 37.0 37.0 37.0 37.0 37.0 70-74 36.3338 37.0 37.0 37.0 37.0 37.0 75-79 36.313900000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.315400000000004 37.0 37.0 37.0 37.0 37.0 85-89 36.2659 37.0 37.0 37.0 37.0 37.0 90-94 36.293600000000005 37.0 37.0 37.0 37.0 37.0 95-99 36.1741 37.0 37.0 37.0 37.0 37.0 100-104 36.2601 37.0 37.0 37.0 37.0 37.0 105-109 36.1948 37.0 37.0 37.0 37.0 37.0 110-114 36.1325 37.0 37.0 37.0 37.0 37.0 115-119 36.1386 37.0 37.0 37.0 37.0 37.0 120-124 36.0865 37.0 37.0 37.0 37.0 37.0 125-129 36.066599999999994 37.0 37.0 37.0 37.0 37.0 130-134 36.0508 37.0 37.0 37.0 37.0 37.0 135-139 36.011 37.0 37.0 37.0 37.0 37.0 140-144 35.8568 37.0 37.0 37.0 37.0 37.0 145-149 35.6545 37.0 37.0 37.0 37.0 37.0 150-151 35.55575 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 0.0 22 0.0 23 1.0 24 0.0 25 4.0 26 2.0 27 5.0 28 9.0 29 15.0 30 31.0 31 34.0 32 40.0 33 70.0 34 104.0 35 304.0 36 2998.0 37 382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.05 11.675 7.575 37.7 2 19.4870505406085 14.609001760120693 37.08825748051295 28.815690218757855 3 17.525 18.15 27.375 36.95 4 22.2 24.2 23.35 30.25 5 22.5 30.9 25.05 21.55 6 23.075000000000003 32.05 24.25 20.625 7 16.1 28.549999999999997 38.975 16.375 8 16.575 28.1 31.825 23.5 9 16.8 25.1 35.099999999999994 23.0 10-14 19.485 30.435000000000002 27.065 23.015 15-19 19.38 28.455000000000002 27.944999999999997 24.22 20-24 20.785 29.515 26.645000000000003 23.055 25-29 20.46 28.285 27.925 23.330000000000002 30-34 20.11 28.12 28.199999999999996 23.57 35-39 19.939999999999998 28.82 27.529999999999998 23.71 40-44 19.564999999999998 28.89 27.455000000000002 24.09 45-49 20.18 28.52 27.315 23.985 50-54 19.915 28.15 27.83 24.104999999999997 55-59 20.025000000000002 28.63 27.095000000000002 24.25 60-64 20.244999999999997 28.155 27.224999999999998 24.375 65-69 19.97 28.345 27.615000000000002 24.07 70-74 19.939999999999998 28.37 27.6 24.09 75-79 19.845 27.525 28.87 23.76 80-84 20.84 28.725 27.49 22.945 85-89 20.3 27.905 27.339999999999996 24.455 90-94 20.669999999999998 27.96 27.815 23.555 95-99 21.14 28.720000000000002 26.99 23.150000000000002 100-104 20.095 28.59 27.474999999999998 23.84 105-109 20.044999999999998 28.34 27.02 24.595 110-114 20.580000000000002 28.96 26.61 23.849999999999998 115-119 21.21 28.165000000000003 26.565 24.060000000000002 120-124 20.66 28.050000000000004 26.86 24.43 125-129 21.33 27.705000000000002 27.505000000000003 23.46 130-134 20.905 28.294999999999998 26.5 24.3 135-139 21.060000000000002 27.944999999999997 26.919999999999998 24.075 140-144 20.74 27.125 27.71 24.425 145-149 20.955 27.71 26.665 24.67 150-151 20.1375 29.2 26.5125 24.15 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 0.5 21 0.0 22 0.0 23 0.5 24 1.5 25 2.0 26 5.0 27 6.5 28 5.0 29 13.0 30 18.5 31 20.0 32 30.0 33 43.0 34 63.0 35 86.0 36 100.0 37 112.5 38 142.0 39 172.5 40 182.5 41 201.5 42 223.5 43 217.0 44 208.5 45 247.5 46 258.0 47 252.0 48 242.0 49 217.0 50 197.0 51 144.5 52 113.0 53 108.0 54 101.0 55 80.5 56 53.5 57 40.0 58 29.0 59 20.0 60 18.0 61 8.0 62 4.5 63 6.0 64 2.5 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.575 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.125 #Duplication Level Percentage of deduplicated Percentage of total 1 71.97860962566844 50.475 2 18.43137254901961 25.85 3 6.631016042780749 13.950000000000001 4 1.7112299465240641 4.8 5 0.8199643493761141 2.875 6 0.24955436720142604 1.05 7 0.07130124777183601 0.35000000000000003 8 0.07130124777183601 0.4 9 0.0 0.0 >10 0.035650623885918005 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGGAGGCCATGGCTAGCTAACTGTACTTTAATTTACAGCAAATACTATA 10 0.25 No Hit CAGCTCCAACAGGAACACCCTCGGCCTTGAGCCTTGATTTCCCATACAGA 8 0.2 No Hit CAAGGTAGGTTGGATCTCCCTTCAATCCTTGAGAAAGGGTTCGGTCTCCA 8 0.2 No Hit GCCTCCAAGTTCGTCCCAAAAATCGATAGACGCCTTCAATCCCACTAGTA 7 0.17500000000000002 No Hit CCCCAACCTCCTCTAACTTCGTAGGAAACAACTGGTTCTTCTTCACGATC 7 0.17500000000000002 No Hit GCTCCCAAACATACTCCTACTGCTTCTTCAATCCCTACTAGATCCATCTT 6 0.15 No Hit ATTAAAGATACATAATTCCATGGAATGGAACCAACAAAGCAGCAGGAAAT 6 0.15 No Hit CAATTTACATAATAAAATACAAGTTGCATTACACACTGTCAAGGGTAGCC 6 0.15 No Hit TGTGTCGCTGCCAATGCTGCTGCCTCCTGTCTTTGCCTCCGACGCTGCAA 6 0.15 No Hit GACAGCATCAAAAATACACAACCTATAACCGAACCAATAGCCGATCCCAT 6 0.15 No Hit GCAACAGCTCCTCATCATCAACTTGATCTTGTTTATTCAAAAACACAACC 6 0.15 No Hit GTCTGTCTTTGGCTTCCCGAAAATATTGGCAGATTCACCATAGGCAGCAT 6 0.15 No Hit GATCAGAGTCTCTAAATTCACTTAATCTTTAATGGAGCTCTCTATACTGC 5 0.125 No Hit CCACAAGCATCTGCAAACTTCAACATATTGGGGAAAATCTCAGACTCGTT 5 0.125 No Hit GTCTTAGCTAACAACCTTTAGGAGCAAATCCAACCATTCCCTTAGCAACA 5 0.125 No Hit GTCCTAACTGTCCCGACTTCTTCTAAAAGCCTTCTTGTCTGAAAGGGCTT 5 0.125 No Hit GTGACCTTGACCCTGAAGACGATGAAGCAGGCGCACTCAATGGTATGCCT 5 0.125 No Hit CTTCATTACAATGTACATATCCATGAGTGAAAAACAATGACAACCTCAAC 5 0.125 No Hit GCCTCTTCCATTCACCACAACCTGGGCCTTCCTGACACTTTGGGTTATCA 5 0.125 No Hit GTCGATATTTAGTCATTGCTAACTCCAGCCATGGCTTCATGTTACCGTTA 5 0.125 No Hit GCTAGTTCTACCGTCATCTTCCTTTTCACATTCACTAGTCTTGCTTTTCC 5 0.125 No Hit GGGGAGAATTTTTTGTTTCTGGACAGTGAATGGGACAGGCTATATGATAC 5 0.125 No Hit GCATCATATTTCAGAACATGAAACTTCATCCCATCAGATCTTCAGATTTT 5 0.125 No Hit GTCAGTCCCATCCCAAGGGAATCGTTGCTGCTGCAGGTCAGCACCCTCCC 5 0.125 No Hit CGCATCTTAATTATATCTATCTACAACAACGCAGCAGGAGGAAGACGATT 5 0.125 No Hit TGAAAATAGAACACGAAATAGGAAAATTCTTGTTCATATCTCTCCTAGAA 5 0.125 No Hit GCACAGGAGAAATTAGAACACGAAGCAAAGTTTGAATTTTCCTTACAGCT 5 0.125 No Hit CTTCTCAACCTTAACTGGAGCCTCTGTGGTGACTTGGGCTCTGATGGAAA 5 0.125 No Hit CATGCCGGAACGGAGGCGAAGAGAGGAGGCGACAGAGCGGCTAACGACGC 5 0.125 No Hit GTCCTCTCAATGCCATGCTCGCCATCAAGTAAGATTTCTGGCTCCACAAT 5 0.125 No Hit GTTCCTTCATCATCTTTGTAGTGTGTCATTACCAATATTTGATGGATAGC 5 0.125 No Hit GCCAAATAGTTCACACGAAGGATTACTGTCAGGTACATGATCTCAGGAAA 5 0.125 No Hit ATCCGCTTTTGCACAAAAGACCATCACATACTATCATGAAAAGATTACAC 5 0.125 No Hit GTGAGTTTCGATGTTCATTTGCATTATGCATCAATGTACGAAACTGGTGA 5 0.125 No Hit GCACAATGAATCCGCAGGCTTGTACTAACTCCTTTGCACGTTCCTTGAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.1125 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.15 0.0 0.0 0.0 0.0 86-87 0.21250000000000002 0.0 0.0 0.0 0.0 88-89 0.35 0.0 0.0 0.0 0.0 90-91 0.4875 0.0 0.0 0.0 0.0 92-93 0.5625 0.0 0.0 0.0 0.0 94-95 0.7 0.0 0.0 0.0 0.0 96-97 0.825 0.0 0.0 0.0 0.0 98-99 1.125 0.0 0.0 0.0 0.0 100-101 1.3125 0.0 0.0 0.0 0.0 102-103 1.5375 0.0 0.0 0.0 0.0 104-105 1.8125 0.0 0.0 0.0 0.0 106-107 2.0125 0.0 0.0 0.0 0.0 108-109 2.2375 0.0 0.0 0.0 0.0 110-111 2.5625 0.0 0.0 0.0 0.0 112-113 2.9375 0.0 0.0 0.0 0.0 114-115 3.25 0.0 0.0 0.0 0.0 116-117 3.5375 0.0 0.0 0.0 0.0 118-119 3.9749999999999996 0.0 0.0 0.0 0.0 120-121 4.3 0.0 0.0 0.0 0.0 122-123 4.725 0.0 0.0 0.0 0.0 124-125 5.6 0.0 0.0 0.0 0.0 126-127 6.275 0.0 0.0 0.0 0.0 128-129 6.95 0.0 0.0 0.0 0.0 130-131 7.5875 0.0 0.0 0.0 0.0 132-133 8.0 0.0 0.0 0.0 0.0 134-135 8.587499999999999 0.0 0.0 0.0 0.0 136-137 9.274999999999999 0.0 0.0 0.0 0.0 138-139 9.9875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGAATT 10 0.006830828 145.0 4 >>END_MODULE SRR13695491 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695491_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.097 37.0 37.0 37.0 37.0 37.0 2 36.212 37.0 37.0 37.0 37.0 37.0 3 36.2095 37.0 37.0 37.0 37.0 37.0 4 36.2225 37.0 37.0 37.0 37.0 37.0 5 36.303 37.0 37.0 37.0 37.0 37.0 6 36.302 37.0 37.0 37.0 37.0 37.0 7 36.23 37.0 37.0 37.0 37.0 37.0 8 36.2515 37.0 37.0 37.0 37.0 37.0 9 36.236 37.0 37.0 37.0 37.0 37.0 10-14 36.340799999999994 37.0 37.0 37.0 37.0 37.0 15-19 36.2435 37.0 37.0 37.0 37.0 37.0 20-24 36.2692 37.0 37.0 37.0 37.0 37.0 25-29 36.1792 37.0 37.0 37.0 37.0 37.0 30-34 36.1838 37.0 37.0 37.0 37.0 37.0 35-39 36.1821 37.0 37.0 37.0 37.0 37.0 40-44 36.1331 37.0 37.0 37.0 37.0 37.0 45-49 36.134 37.0 37.0 37.0 37.0 37.0 50-54 36.06569999999999 37.0 37.0 37.0 37.0 37.0 55-59 36.0785 37.0 37.0 37.0 37.0 37.0 60-64 35.99470000000001 37.0 37.0 37.0 37.0 37.0 65-69 35.9703 37.0 37.0 37.0 37.0 37.0 70-74 35.9747 37.0 37.0 37.0 37.0 37.0 75-79 35.9277 37.0 37.0 37.0 37.0 37.0 80-84 35.978899999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.869600000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.8321 37.0 37.0 37.0 37.0 37.0 95-99 35.8759 37.0 37.0 37.0 37.0 37.0 100-104 35.881499999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.8493 37.0 37.0 37.0 37.0 37.0 110-114 35.712199999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.6819 37.0 37.0 37.0 37.0 37.0 120-124 35.6804 37.0 37.0 37.0 37.0 37.0 125-129 35.7072 37.0 37.0 37.0 37.0 37.0 130-134 35.587300000000006 37.0 37.0 37.0 37.0 37.0 135-139 35.5595 37.0 37.0 37.0 37.0 37.0 140-144 35.4527 37.0 37.0 37.0 37.0 37.0 145-149 35.34055 37.0 37.0 37.0 34.6 37.0 150-151 35.003375 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 5.0 15 1.0 16 1.0 17 4.0 18 4.0 19 0.0 20 4.0 21 5.0 22 2.0 23 4.0 24 6.0 25 8.0 26 9.0 27 10.0 28 13.0 29 15.0 30 20.0 31 34.0 32 45.0 33 89.0 34 184.0 35 529.0 36 2797.0 37 211.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.405920722528855 21.876567987957852 11.214249874560963 29.50326141495233 2 27.825 25.174999999999997 29.175 17.825 3 20.549999999999997 26.150000000000002 33.324999999999996 19.975 4 24.099999999999998 34.75 22.2 18.95 5 26.700000000000003 35.75 21.05 16.5 6 20.025000000000002 39.95 21.75 18.275 7 19.75 24.25 37.375 18.625 8 21.3 25.4 28.4 24.9 9 23.474999999999998 25.174999999999997 28.9 22.45 10-14 22.900000000000002 29.95 26.424999999999997 20.724999999999998 15-19 23.035 27.68 28.044999999999998 21.240000000000002 20-24 23.755000000000003 27.97 27.42 20.855 25-29 22.759999999999998 27.91 27.47 21.86 30-34 23.325000000000003 27.800000000000004 27.93 20.945 35-39 23.165 28.605000000000004 27.224999999999998 21.005 40-44 23.13 28.76 27.150000000000002 20.96 45-49 22.725 28.144999999999996 28.505000000000003 20.625 50-54 22.925 28.22 28.060000000000002 20.794999999999998 55-59 24.044999999999998 27.839999999999996 27.12 20.995 60-64 23.275000000000002 27.83 27.395000000000003 21.5 65-69 23.79 27.095000000000002 28.244999999999997 20.87 70-74 23.41 27.939999999999998 27.229999999999997 21.42 75-79 22.994999999999997 28.255000000000003 27.16 21.59 80-84 22.835 27.575 27.955000000000002 21.634999999999998 85-89 24.175 27.534999999999997 27.115000000000002 21.175 90-94 24.044999999999998 27.200000000000003 28.065 20.69 95-99 24.07 27.779999999999998 27.71 20.44 100-104 24.884999999999998 28.7 26.76 19.655 105-109 23.315 28.794999999999998 27.060000000000002 20.830000000000002 110-114 23.79 27.694999999999997 28.475 20.04 115-119 23.395 28.43 27.950000000000003 20.225 120-124 24.865000000000002 28.275 26.85 20.01 125-129 24.555 28.015 27.415 20.015 130-134 25.470094018803763 28.325665133026607 26.59531906381276 19.608921784356873 135-139 25.28252825282528 28.277827782778274 26.607660766076606 19.831983198319833 140-144 25.540000000000003 27.04 27.52 19.900000000000002 145-149 26.376318815940795 27.486374318715935 26.45632281614081 19.68098404920246 150-151 26.35988495685882 28.298111791921972 25.609603601350507 19.7323996498687 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 1.5 12 1.5 13 1.0 14 2.5 15 3.0 16 1.0 17 0.5 18 0.5 19 0.0 20 1.5 21 1.5 22 0.5 23 0.5 24 0.0 25 1.5 26 2.5 27 6.0 28 6.5 29 5.0 30 9.0 31 11.5 32 20.0 33 29.0 34 35.0 35 57.5 36 90.5 37 109.5 38 126.0 39 174.5 40 207.5 41 219.0 42 242.5 43 257.0 44 258.0 45 262.5 46 266.0 47 269.5 48 243.5 49 210.5 50 187.5 51 144.0 52 113.0 53 86.0 54 79.0 55 73.5 56 49.5 57 34.0 58 22.5 59 19.5 60 19.5 61 10.5 62 6.5 63 4.5 64 1.0 65 0.5 66 1.0 67 1.0 68 0.5 69 0.5 70 0.0 71 0.0 72 0.5 73 0.5 74 0.5 75 1.0 76 0.5 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 1.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.02 135-139 0.01 140-144 0.0 145-149 0.005 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.775 #Duplication Level Percentage of deduplicated Percentage of total 1 73.08371600141292 51.725 2 17.732250088308017 25.1 3 6.216884493111975 13.200000000000001 4 1.7308371600141295 4.9 5 0.7064641469445425 2.5 6 0.282585658777817 1.2 7 0.1059696220416814 0.525 8 0.1059696220416814 0.6 9 0.0 0.0 >10 0.035323207347227124 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGCAATGGATGCAGCATGTACCCAGACTTGAGTTTCTCCGAGACCACCA 10 0.25 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 8 0.2 No Hit GTTGAAAAAGCTGGGGGTTACAGTAGTGATGGTCATAAGTCTGTGCTAGA 8 0.2 No Hit CACAAATTACTTTTCCTTGTTGTAATTGGAAAATCTTAACAGACTAGGAG 8 0.2 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 7 0.17500000000000002 No Hit CATTGGTGTTGTGTCAACCAAAGAACCCTTCAAGTGCGTCATTAACCAGG 7 0.17500000000000002 No Hit GCAAGCACGAGGGAGCTCCGTTGATGTTTCCAGGTACTAAAGAGGCGTGG 7 0.17500000000000002 No Hit GTAGTGAAGACTAGTTCAAGAAAGGACAGTGGAGATCATGATGATCAGGT 6 0.15 No Hit CACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCCTC 6 0.15 No Hit AGAAAACGTCGCCGCATGGCTCCAACCAAGCAGTTTTATGGAACTATAAT 6 0.15 No Hit GCAGGAGCAGCTTCATAAACTGCCAAAGCCTTTCCCTATTTTGAAGATCA 6 0.15 No Hit TCTCGCCATTATGCTCATGTTGATTGCCCTGGTCATGCCGATTATGTTAA 6 0.15 No Hit TACTTCAAATGCTAGTGGAGCTGCATTTTGCCGTTCAGTTGCTCTTATCT 6 0.15 No Hit CTTAGCAAGTAGTTTGCTTCTTTGAAGGACCAAGGATTCAGATTTGTCAT 6 0.15 No Hit TCTAGATCCTCCATTTCAACGACCAGCGTGCACGTGACAGCCCTTGATGG 6 0.15 No Hit CCCGAACACACACCCAGTCCCGCTCTCCTTGTTCCCGCTCACCAAGGGTA 5 0.125 No Hit GGCAACATTGATGATGCTTTGGCAAAGCTACAGGCAATCATTGATGTGGC 5 0.125 No Hit ACCAGTTTGCTGGATGAGGAAACCGGGGATGCAGGAGAAGAAGATTTGGA 5 0.125 No Hit CTTCAGGCTAGACTTTGCGGTAAGTAGAGAGCAAACCAATGCCAAAGGTG 5 0.125 No Hit AGGGAGTGAAAAGCAAAAGAGACCATGGCCTCCACTTCATGCTTCTTGCA 5 0.125 No Hit CTGCAATGGTACACCCCCTTCCGGGTCACTCTTGAGAGTCTTGAGATTGC 5 0.125 No Hit ACACATTGACAGGATCACCATGTGTCAATTTCATTTCACAATCTATGAAT 5 0.125 No Hit AATCACCAGATAGGTAGTGAAGATTGGCTGACAACAACAATAGTACTTCA 5 0.125 No Hit GCCGTGTTCTGGATGAGGGCGTTGTGGTTCGAGCTTCGGACCTCGACACC 5 0.125 No Hit GTTGTGTCCGAATGAAATTTGGGGACATGCCAGCTGTGGTGAGGATCGAA 5 0.125 No Hit ATTACAATGAAGCTGCTGCGAAACTTGAAAACATATACAAGCTTAGCCCT 5 0.125 No Hit TGAAGATCAAGAGGAGCAGAGATGTGGTCAAGTTCAAAGTTCGCTGCTCA 5 0.125 No Hit GCTACTAACTAGTCTATCAAAGATCAAATATATGGCTCCACTTCCTAGCA 5 0.125 No Hit AAGTGTCGATGTGGTGACCTCTTTTGTGCATCTCATCGCTACTCAGACAA 5 0.125 No Hit GCACCACCTACCCCACAGCACCCGCTCTCTCCAATGGGTGATGCTTGTAC 5 0.125 No Hit GGGTTTTTCATGGCTTACCTGGTTGATAGCTTGACTGGTGTTGGTCTGGT 5 0.125 No Hit TGGAGTTGAAGTGGAAATCGATGTTTCGGGGATCATGATCCCTGTGAATG 5 0.125 No Hit GTCTCCTTTCCTTCCTCTACCAAGTCTACCTCTCTTTCTTCAAGAACCAC 5 0.125 No Hit GGAACCGGCTCTACCGCCGCCTTCGTGGTAGCCAAACTCGGCGAGCTCTT 5 0.125 No Hit AATCTCCCTATCAAGATAATGCTTCTGAACAATCAGCATTTGGGCATGGT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.1125 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.15 0.0 0.0 0.0 0.0 86-87 0.21250000000000002 0.0 0.0 0.0 0.0 88-89 0.35 0.0 0.0 0.0 0.0 90-91 0.4875 0.0 0.0 0.0 0.0 92-93 0.5625 0.0 0.0 0.0 0.0 94-95 0.7 0.0 0.0 0.0 0.0 96-97 0.8374999999999999 0.0 0.0 0.0 0.0 98-99 1.1375000000000002 0.0 0.0 0.0 0.0 100-101 1.3125 0.0 0.0 0.0 0.0 102-103 1.5375 0.0 0.0 0.0 0.0 104-105 1.825 0.0 0.0 0.0 0.0 106-107 2.0375 0.0 0.0 0.0 0.0 108-109 2.2625 0.0 0.0 0.0 0.0 110-111 2.5875000000000004 0.0 0.0 0.0 0.0 112-113 2.9375 0.0 0.0 0.0 0.0 114-115 3.25 0.0 0.0 0.0 0.0 116-117 3.5375 0.0 0.0 0.0 0.0 118-119 3.9749999999999996 0.0 0.0 0.0 0.0 120-121 4.3625 0.0 0.0 0.0 0.0 122-123 4.8 0.0 0.0 0.0 0.0 124-125 5.675 0.0 0.0 0.0 0.0 126-127 6.35 0.0 0.0 0.0 0.0 128-129 6.9625 0.0 0.0 0.0 0.0 130-131 7.5875 0.0 0.0 0.0 0.0 132-133 7.975 0.0 0.0 0.0 0.0 134-135 8.5625 0.0 0.0 0.0 0.0 136-137 9.225000000000001 0.0 0.0 0.0 0.0 138-139 9.9375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCTGAA 10 0.006830828 145.0 145 AGATCCT 10 0.006830828 145.0 4 >>END_MODULE Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702761 spots for SRR13695491.sra Written 702761 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra Read 702747 spots for SRR13695491.sra Written 702747 spots for SRR13695491.sra SRR ids: ['SRR13695491.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_uifu9p6k SRR13695491.sra spots: 14054954 blocks: [[1, 702747], [702748, 1405494], [1405495, 2108241], [2108242, 2810988], [2810989, 3513735], [3513736, 4216482], [4216483, 4919229], [4919230, 5621976], [5621977, 6324723], [6324724, 7027470], [7027471, 7730217], [7730218, 8432964], [8432965, 9135711], [9135712, 9838458], [9838459, 10541205], [10541206, 11243952], [11243953, 11946699], [11946700, 12649446], [12649447, 13352193], [13352194, 14054954]] SRR13695491 file size 4754787 SRR13695491 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695491 SRR13695491_1.fastq SRR13695491_2.fastq Input file: SRR13695491_1.fastq Paired file: SRR13695491_2.fastq trimmed: SRR13695491-trimmed-pair1.fastq, SRR13695491-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 07:09:04 2025 >> started Wed Feb 12 07:09:21 2025 >> done (16.510s) 14054954 read pairs processed; of these: 95 ( 0.00%) short read pairs filtered out after trimming by size control 873 ( 0.01%) empty read pairs filtered out after trimming by size control 14053986 (99.99%) read pairs available; of these: 1862624 (13.25%) trimmed read pairs available after processing 12191362 (86.75%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 0 0.00% 20 1 0.00% 21 1 0.00% 22 0 0.00% 23 4 0.00% 24 3 0.00% 25 2 0.00% 26 1 0.00% 27 1 0.00% 28 3 0.00% 29 1 0.00% 30 3 0.00% 31 6 0.00% 32 4 0.00% 33 2 0.00% 34 0 0.00% 35 3 0.00% 36 5 0.00% 37 4 0.00% 38 8 0.00% 39 3 0.00% 40 8 0.00% 41 11 0.00% 42 20 0.00% 43 11 0.00% 44 7 0.00% 45 18 0.00% 46 23 0.00% 47 18 0.00% 48 24 0.00% 49 39 0.00% 50 40 0.00% 51 46 0.00% 52 61 0.00% 53 59 0.00% 54 64 0.00% 55 71 0.00% 56 90 0.00% 57 148 0.00% 58 140 0.00% 59 191 0.00% 60 220 0.00% 61 299 0.00% 62 309 0.00% 63 403 0.00% 64 403 0.00% 65 451 0.00% 66 509 0.00% 67 614 0.00% 68 709 0.01% 69 833 0.01% 70 1006 0.01% 71 1165 0.01% 72 1285 0.01% 73 1586 0.01% 74 1734 0.01% 75 1929 0.01% 76 2174 0.02% 77 2388 0.02% 78 2631 0.02% 79 2947 0.02% 80 3334 0.02% 81 3811 0.03% 82 4184 0.03% 83 4671 0.03% 84 5313 0.04% 85 5822 0.04% 86 6321 0.04% 87 6591 0.05% 88 6928 0.05% 89 7475 0.05% 90 8203 0.06% 91 8443 0.06% 92 9365 0.07% 93 9972 0.07% 94 10675 0.08% 95 11620 0.08% 96 11792 0.08% 97 12824 0.09% 98 13305 0.09% 99 13965 0.10% 100 14614 0.10% 101 14956 0.11% 102 15639 0.11% 103 16502 0.12% 104 17405 0.12% 105 18144 0.13% 106 18764 0.13% 107 19576 0.14% 108 20395 0.15% 109 20995 0.15% 110 21399 0.15% 111 21847 0.16% 112 22979 0.16% 113 23012 0.16% 114 24520 0.17% 115 25320 0.18% 116 26994 0.19% 117 26940 0.19% 118 27978 0.20% 119 28349 0.20% 120 29104 0.21% 121 29570 0.21% 122 30197 0.21% 123 31381 0.22% 124 32237 0.23% 125 32550 0.23% 126 34277 0.24% 127 35586 0.25% 128 35330 0.25% 129 36527 0.26% 130 37228 0.26% 131 37531 0.27% 132 37778 0.27% 133 39011 0.28% 134 38944 0.28% 135 40170 0.29% 136 41131 0.29% 137 41889 0.30% 138 42556 0.30% 139 43815 0.31% 140 44125 0.31% 141 44462 0.32% 142 45222 0.32% 143 45450 0.32% 144 46519 0.33% 145 47179 0.34% 146 47303 0.34% 147 48639 0.35% 148 49469 0.35% 149 50606 0.36% 150 51155 0.36% 151 12191362 86.75% 14053986 reads passed initial QC criterion=sequence-density sequence-density=0.62 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=21 prefix-density=0.61 prefix-fanout=2.0 sequence=GTGTTGTCGAATCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=26 fanout-score=36.40 fanout-score-rank=1 prefix-density=0.07 prefix-fanout=3.6 sequence=TAAAAGCAGAATCAAGCTTCCGATTAAAGATACATAATTCCATGGAATGGAACCAACAAAGCAGCAGGAAATACAAGACACTTACAGATTACTAGCCATCAAATGAGATCCTGTAGAAAGGATTTGAGGAGGCCATGGCTAGCTAACTGTACTTTAATTTACAGCAAATACTATATTAGACAAACATGGAGTGACCAGACTTCTTCTTTTTATTTATTATAGTTCCATAAAACTGCTTGGTTGGAGCCATGCGGCGACGTTTTCTCATTTGCAGGAGCATGGATCACAGGTGCAGTTTGATCCACATTTGCAGCCATTCTCAGCACCAAAGTTCATCTCAGAGCTCTCGTAGAACATCCTAACTGGAGCTACACCAGCAATGATTGTCTGACTTGTGGTGGTCTCGGAGAAACTCAAGTCTGGGTACATGCTGCATCCATTGCAGCCACTGCCGCACTTGCA criterion=sequence-density sequence-density=0.81 sequence-density-rank=1 fanout-score=2.14 fanout-score-rank=21 prefix-density=0.83 prefix-fanout=2.1 sequence=CCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.02 sequence-density-rank=27 fanout-score=18.79 fanout-score-rank=1 prefix-density=0.06 prefix-fanout=6.0 sequence=ACTGAGCTTGAGTGCATTCTTCTAAGTAAAAGAAATCCCTTAATTTCATCA SRR13695491 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 07:10:02 Started mapping on | Feb 12 07:10:02 Finished on | Feb 12 07:11:26 Mapping speed, Million of reads per hour | 602.31 Number of input reads | 14053986 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 13323405 Uniquely mapped reads % | 94.80% Average mapped length | 293.97 Number of splices: Total | 13114603 Number of splices: Annotated (sjdb) | 12866004 Number of splices: GT/AG | 12834449 Number of splices: GC/AG | 229504 Number of splices: AT/AC | 7378 Number of splices: Non-canonical | 43272 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.87 Insertion rate per base | 0.02% Insertion average length | 2.37 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 298216 % of reads mapped to multiple loci | 2.12% Number of reads mapped to too many loci | 31230 % of reads mapped to too many loci | 0.22% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.76% % of reads unmapped: other | 0.10% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 432542 432542 432542 N_multimapping 298216 298216 298216 N_noFeature 385725 12961708 575785 N_ambiguous 257157 1387 84513 UnstrandedReadsAssigned:12680523 PositiveStrandReadsAssigned:360310 NegativeStrandReadsAssigned:12663107 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695491 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695491-trimmed-pair1.fastq SRR13695491-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 14,053,986 reads, 12,673,346 reads pseudoaligned [quant] estimated average fragment length: 242.322 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,090 rounds 52401 SRR13695491.ke.tsv 34699 SRR13695491.se.tsv 87100 total ==> SRR13695491.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1776.68 372 13.0783 Potri.005G024800.1.v4.1 1035 793.678 267 21.0129 Potri.004G059700.1.v4.1 961 719.759 9 0.781041 Potri.007G009000.2.v4.1 1416 1174.68 0 0 Potri.003G141000.2.v4.1 2943 2701.68 756 17.4786 Potri.016G087400.1.v4.1 270 86.4059 896 647.714 Potri.015G069301.1.v4.1 564 330.298 0 0 Potri.010G195200.1.v4.1 1773 1531.68 51 2.0798 Potri.012G127500.1.v4.1 977 735.716 55 4.66951 ==> SRR13695491.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 97 Potri.001G233950.v4.1 1 Potri.001G122700.v4.1 244 Potri.001G212900.v4.1 3 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 3 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 10 SRR13695491 completed mapping pipeline successfully