Starting /dee2/code/volunteer_pipeline.sh SRR13695492 current disk space = 3049887723520 free memory = 1575395948 SRR13695492 SRAfilesize 9f6f97d3c182f613814298162325f91a SRR13695492.sra SRR13695492.sra file validated SRR13695492 is paired end SRR13695492 is conventional basespace SRR13695492 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695492_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.4705 37.0 37.0 37.0 37.0 37.0 2 36.05075 37.0 37.0 37.0 37.0 37.0 3 36.5035 37.0 37.0 37.0 37.0 37.0 4 36.548 37.0 37.0 37.0 37.0 37.0 5 36.539 37.0 37.0 37.0 37.0 37.0 6 36.5695 37.0 37.0 37.0 37.0 37.0 7 36.4505 37.0 37.0 37.0 37.0 37.0 8 36.565 37.0 37.0 37.0 37.0 37.0 9 36.4865 37.0 37.0 37.0 37.0 37.0 10-14 36.532700000000006 37.0 37.0 37.0 37.0 37.0 15-19 36.4838 37.0 37.0 37.0 37.0 37.0 20-24 36.474199999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.4138 37.0 37.0 37.0 37.0 37.0 30-34 36.395300000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.3815 37.0 37.0 37.0 37.0 37.0 40-44 36.425799999999995 37.0 37.0 37.0 37.0 37.0 45-49 36.352700000000006 37.0 37.0 37.0 37.0 37.0 50-54 36.3152 37.0 37.0 37.0 37.0 37.0 55-59 36.311899999999994 37.0 37.0 37.0 37.0 37.0 60-64 36.2832 37.0 37.0 37.0 37.0 37.0 65-69 36.28000000000001 37.0 37.0 37.0 37.0 37.0 70-74 36.2743 37.0 37.0 37.0 37.0 37.0 75-79 36.2387 37.0 37.0 37.0 37.0 37.0 80-84 36.1712 37.0 37.0 37.0 37.0 37.0 85-89 36.130900000000004 37.0 37.0 37.0 37.0 37.0 90-94 36.040800000000004 37.0 37.0 37.0 37.0 37.0 95-99 36.0532 37.0 37.0 37.0 37.0 37.0 100-104 35.9825 37.0 37.0 37.0 37.0 37.0 105-109 36.083800000000004 37.0 37.0 37.0 37.0 37.0 110-114 36.0539 37.0 37.0 37.0 37.0 37.0 115-119 36.0563 37.0 37.0 37.0 37.0 37.0 120-124 35.8917 37.0 37.0 37.0 37.0 37.0 125-129 35.8779 37.0 37.0 37.0 37.0 37.0 130-134 35.887299999999996 37.0 37.0 37.0 37.0 37.0 135-139 35.7095 37.0 37.0 37.0 37.0 37.0 140-144 35.642 37.0 37.0 37.0 37.0 37.0 145-149 35.519099999999995 37.0 37.0 37.0 37.0 37.0 150-151 35.43875 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 1.0 24 2.0 25 4.0 26 5.0 27 14.0 28 15.0 29 25.0 30 28.0 31 37.0 32 51.0 33 78.0 34 133.0 35 331.0 36 2961.0 37 313.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.375 11.825 9.125 38.675 2 20.65845689871827 13.722040713747171 38.602663985926114 27.016838401608446 3 19.0 18.425 26.575 36.0 4 23.575 27.3 22.0 27.125 5 24.5 31.225 23.674999999999997 20.599999999999998 6 20.5 36.3 23.849999999999998 19.35 7 17.075000000000003 26.674999999999997 40.1 16.150000000000002 8 16.950000000000003 27.250000000000004 34.225 21.575 9 18.05 23.425 35.775 22.75 10-14 20.455000000000002 30.11 26.41 23.025000000000002 15-19 19.985 27.750000000000004 28.92 23.345 20-24 20.405 28.415000000000003 28.54 22.64 25-29 20.205000000000002 28.58 27.905 23.31 30-34 20.22 28.025 28.015 23.74 35-39 20.215 28.675 28.18 22.93 40-44 20.215 29.015 27.544999999999998 23.225 45-49 19.98 28.610000000000003 27.615000000000002 23.794999999999998 50-54 20.275000000000002 28.999999999999996 27.445000000000004 23.28 55-59 20.655 29.299999999999997 26.955000000000002 23.09 60-64 19.84 29.84 27.875 22.445 65-69 20.32 28.065 28.46 23.155 70-74 20.77 28.199999999999996 27.87 23.16 75-79 20.035 29.015 27.255000000000003 23.695 80-84 19.88 29.23 28.1 22.79 85-89 20.755000000000003 28.675 27.275 23.294999999999998 90-94 20.145 28.860000000000003 28.060000000000002 22.935 95-99 20.755000000000003 28.15 28.64 22.455 100-104 20.365 29.134999999999998 27.965 22.535 105-109 21.09 29.025000000000002 27.46 22.425 110-114 20.380000000000003 28.615000000000002 27.87 23.135 115-119 21.845 28.815 26.355 22.985 120-124 20.794999999999998 29.74 27.045 22.42 125-129 20.41 28.955 27.52 23.115 130-134 20.29 28.425 28.325 22.96 135-139 20.02 29.515 27.169999999999998 23.294999999999998 140-144 21.495 28.77 27.185 22.55 145-149 20.815 28.18 27.255000000000003 23.75 150-151 20.3375 27.737499999999997 27.962500000000002 23.962500000000002 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.5 14 1.5 15 0.0 16 0.0 17 0.5 18 0.5 19 0.5 20 1.0 21 1.5 22 1.5 23 1.5 24 3.5 25 5.5 26 8.0 27 8.0 28 10.5 29 19.5 30 21.0 31 24.0 32 35.5 33 49.5 34 69.5 35 85.0 36 107.5 37 122.0 38 136.0 39 155.0 40 184.0 41 225.5 42 241.5 43 239.5 44 259.0 45 262.0 46 255.5 47 245.5 48 216.0 49 186.5 50 157.5 51 135.0 52 115.0 53 100.0 54 78.0 55 60.5 56 51.0 57 36.5 58 20.5 59 16.5 60 12.0 61 13.0 62 9.5 63 1.5 64 2.5 65 3.0 66 1.0 67 0.0 68 0.5 69 0.5 70 0.0 71 0.0 72 0.0 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.525 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.175 #Duplication Level Percentage of deduplicated Percentage of total 1 70.00366703337 47.725 2 19.545287862119544 26.650000000000002 3 6.417308397506417 13.125 4 2.6402640264026402 7.199999999999999 5 0.77007700770077 2.625 6 0.44004400440044 1.7999999999999998 7 0.18335166850018336 0.8750000000000001 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCCTTTTTGCCACTTTCAACTGATTCAGCCTTTGTCGAACCATCTATTT 7 0.17500000000000002 No Hit CCCCACACAGCAGCGCATATAGAAACAACACTGCACAAAAAAATAAGGAA 7 0.17500000000000002 No Hit GTGAACTTGACCTCAATTGGGCTAACGTTCTTTCCGATTGACCTGAAATT 7 0.17500000000000002 No Hit ATCGGCTGTCACAATTTGAAGAGATGGGTTGTGTTCAGAAAGAATAGTTT 7 0.17500000000000002 No Hit GCCATCCAAGCCAAATCTCTCTGGCACAAACTCTTCTGCTCTCTCCCAGA 7 0.17500000000000002 No Hit TGTAGATCACGAGCAAGCATTGATATTCCCTTGACATTAGGAGAGTTAAC 6 0.15 No Hit TGTGGATCTGCCCAGTAGCTGTATGTCCCGGCCGGTGGGATGACACCAGT 6 0.15 No Hit CCCAGGTGTGATGAGGCCTTTGGCTTCTGCATCAGCAATCATGCTATAGC 6 0.15 No Hit CCAGAGACGAAGACTAATGGTGTTCTTGGTTTTGTATCCTGGAATTGGCA 6 0.15 No Hit CTACAATTAAATTAAAACCTAGAGAGATCAACAAAACACACAGAGGATTC 6 0.15 No Hit GTAAGCTATAGTAAATTAAAAAAAGATTCAATATTTTCTAGGCAGACAGA 6 0.15 No Hit AAGGAAGGTTTATGACATAGAGAAGGAAAAATGCCCAGGTGACACCAGAA 6 0.15 No Hit GTGAATTTTGGATAGATTTGCCAGTTGATGATGCTGCCAGAAATGATGTC 6 0.15 No Hit CTCTGAGCATGGATGAGGTTGGGGTTGCCAAGGTAATCAAGGCCACCCTC 6 0.15 No Hit TCTTGATGTCATAGCAGGAGTACCGATTCTCACTCCTCCAGGACAGATAG 6 0.15 No Hit CCTTATTATATCATCCAATACTGCCTTATCCATCATCCCTTCCATCGTCA 6 0.15 No Hit GGGTTTCCTAGCATTTCAGACACTCCAAGGGAGGCAGCTGCTCTGGATGC 6 0.15 No Hit CCTGATAAACCTGCTTTTCTGCCTCCGTGAGGTTGTTCCATAAGAGTCCA 5 0.125 No Hit AGACAAAGGCTTTGTTTGAGGGATCCGGGTTGGTGTTCCAGTGTCTGCCT 5 0.125 No Hit GTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTT 5 0.125 No Hit GTGGATATGAATCGACGGTTGGTAGTGAGAGCGTGGCGAGAAAGAGAGGG 5 0.125 No Hit ATTCAAGTACAGATAACAGGCATTCTCCCTTCTACAACTAGTAACTTCTT 5 0.125 No Hit CATGATCTTTCTGCTCCGCTGAGAAGTACACCTTTTTGCTAGCATCTCGC 5 0.125 No Hit GTTTGCTACGGGTCCGGTGGCTAGGCCTGAGTAGTGGACGAGAATAGCGG 5 0.125 No Hit GCCCGATCGAACCCTTGCCGGTAAACTCATCGATGATCTTCCCCTTCGTT 5 0.125 No Hit GCCTCAAGTTATTGCCCTAATTTCATTCTATACAGCTAGCTTCACACGGA 5 0.125 No Hit CTTGTTCACTATTAGCCAGAGTATAGAGCATATCATTGGGGATGCCCACC 5 0.125 No Hit ATAGGAAAGGCAATATCATTAAAAATAAATTGTAAAAAGGACCTCATAAG 5 0.125 No Hit CTCCCTCGTGCTCCAGGAGAATTCATCAGGTAGTTCTTTAACTATAGAAA 5 0.125 No Hit GGCCCCCAACATGAGCTATGTCCCCAATGGAGATATACCCGTCTGGACAA 5 0.125 No Hit GTCCGTCGCTGCTGCTTGTTGTTGCCATTTCCAAAATGCGGGAATTCACA 5 0.125 No Hit CTCATACTCTAATTATTATTATTACTATTCCTTCAAATTGGATTCTCTTC 5 0.125 No Hit GTCCTACCAGGAGGAAGCCATGGGCCAGTCCAACCTCGATTCCTCGGAGA 5 0.125 No Hit TGCGTGAAGCAACAACTACATGATCACCAGAAAGAAGAAATTCTCGAGCA 5 0.125 No Hit GGACCATATTCGCAACATTGCGGAGCACAAAAGCTTCCCCTGGTTGGAAA 5 0.125 No Hit CTGGGAGACACCCTCAATTATCCAACATTCAACTGACCAGCAAGCCATGT 5 0.125 No Hit GGCGAATTTGGACTTTGAAGATGAAAGAAGTGAAGGGAAAGCGGTGGCAA 5 0.125 No Hit GTTAGAGTGGAGCTGACCTGGTGGCCGTGGCGGTGGTTGGTGGTGGTGGA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.275 0.0 0.0 0.0 0.0 84-85 0.2875 0.0 0.0 0.0 0.0 86-87 0.4125 0.0 0.0 0.0 0.0 88-89 0.625 0.0 0.0 0.0 0.0 90-91 0.7875 0.0 0.0 0.0 0.0 92-93 1.0125000000000002 0.0 0.0 0.0 0.0 94-95 1.1375 0.0 0.0 0.0 0.0 96-97 1.35 0.0 0.0 0.0 0.0 98-99 1.4249999999999998 0.0 0.0 0.0 0.0 100-101 1.4874999999999998 0.0 0.0 0.0 0.0 102-103 1.55 0.0 0.0 0.0 0.0 104-105 1.65 0.0 0.0 0.0 0.0 106-107 1.7875 0.0 0.0 0.0 0.0 108-109 2.0 0.0 0.0 0.0 0.0 110-111 2.3875 0.0 0.0 0.0 0.0 112-113 2.6500000000000004 0.0 0.0 0.0 0.0 114-115 2.8125 0.0 0.0 0.0 0.0 116-117 3.0125 0.0 0.0 0.0 0.0 118-119 3.45 0.0 0.0 0.0 0.0 120-121 3.7625 0.0 0.0 0.0 0.0 122-123 3.9749999999999996 0.0 0.0 0.0 0.0 124-125 4.35 0.0 0.0 0.0 0.0 126-127 4.85 0.0 0.0 0.0 0.0 128-129 5.2875 0.0 0.0 0.0 0.0 130-131 5.85 0.0 0.0 0.0 0.0 132-133 6.275 0.0 0.0 0.0 0.0 134-135 6.7125 0.0 0.0 0.0 0.0 136-137 7.237500000000001 0.0 0.0 0.0 0.0 138-139 7.75 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCAT 10 0.006830828 145.0 5 >>END_MODULE SRR13695492 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695492_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.8675 37.0 37.0 37.0 37.0 37.0 2 36.0365 37.0 37.0 37.0 37.0 37.0 3 36.1285 37.0 37.0 37.0 37.0 37.0 4 36.1765 37.0 37.0 37.0 37.0 37.0 5 36.156 37.0 37.0 37.0 37.0 37.0 6 36.1665 37.0 37.0 37.0 37.0 37.0 7 36.122 37.0 37.0 37.0 37.0 37.0 8 36.2335 37.0 37.0 37.0 37.0 37.0 9 36.1985 37.0 37.0 37.0 37.0 37.0 10-14 36.15559999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.1481 37.0 37.0 37.0 37.0 37.0 20-24 36.12115 37.0 37.0 37.0 37.0 37.0 25-29 36.07185 37.0 37.0 37.0 37.0 37.0 30-34 36.00515 37.0 37.0 37.0 37.0 37.0 35-39 35.960750000000004 37.0 37.0 37.0 37.0 37.0 40-44 35.958749999999995 37.0 37.0 37.0 37.0 37.0 45-49 35.96065 37.0 37.0 37.0 37.0 37.0 50-54 35.862350000000006 37.0 37.0 37.0 37.0 37.0 55-59 35.89225 37.0 37.0 37.0 37.0 37.0 60-64 35.837149999999994 37.0 37.0 37.0 37.0 37.0 65-69 35.8487 37.0 37.0 37.0 37.0 37.0 70-74 35.78495 37.0 37.0 37.0 37.0 37.0 75-79 35.817550000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.81845 37.0 37.0 37.0 37.0 37.0 85-89 35.729949999999995 37.0 37.0 37.0 37.0 37.0 90-94 35.652049999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.62505 37.0 37.0 37.0 37.0 37.0 100-104 35.70115 37.0 37.0 37.0 37.0 37.0 105-109 35.51775 37.0 37.0 37.0 37.0 37.0 110-114 35.51965 37.0 37.0 37.0 37.0 37.0 115-119 35.45415 37.0 37.0 37.0 37.0 37.0 120-124 35.40145 37.0 37.0 37.0 34.6 37.0 125-129 35.37995 37.0 37.0 37.0 34.6 37.0 130-134 35.22474999999999 37.0 37.0 37.0 32.2 37.0 135-139 35.1837 37.0 37.0 37.0 32.2 37.0 140-144 35.08095 37.0 37.0 37.0 25.0 37.0 145-149 34.96555 37.0 37.0 37.0 25.0 37.0 150-151 34.728624999999994 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 4.0 14 5.0 15 4.0 16 0.0 17 0.0 18 0.0 19 1.0 20 0.0 21 0.0 22 4.0 23 2.0 24 5.0 25 7.0 26 17.0 27 7.0 28 20.0 29 26.0 30 44.0 31 51.0 32 65.0 33 125.0 34 246.0 35 634.0 36 2551.0 37 180.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.68891118916207 20.572002007024587 12.318113396889112 27.420973406924237 2 27.150000000000002 24.375 32.0 16.475 3 22.675 25.4 32.475 19.45 4 23.05 33.125 24.4 19.425 5 24.575 37.7 22.75 14.975 6 19.775000000000002 42.0 20.95 17.275 7 20.599999999999998 22.400000000000002 38.475 18.525 8 20.7 25.2 29.049999999999997 25.05 9 21.175 23.925 31.374999999999996 23.525 10-14 22.53 30.245 26.47 20.755000000000003 15-19 23.06 28.29 28.33 20.32 20-24 22.73068267066767 28.367091772943237 28.272068017004255 20.630157539384847 25-29 22.060515128782196 28.667166791697923 28.782195548887223 20.49012253063266 30-34 22.8607151787947 27.886971742935735 27.901975493873472 21.350337584396097 35-39 22.330582645661416 27.901975493873472 28.402100525131285 21.365341335333834 40-44 21.91547886971743 28.162040510127532 28.9472368092023 20.975243810952737 45-49 22.260565141285323 28.912228057014254 28.047011752938232 20.78019504876219 50-54 22.600650162540635 28.29707426856714 28.612153038259564 20.49012253063266 55-59 22.260565141285323 28.782195548887223 27.886971742935735 21.070267566891722 60-64 22.550637659414853 28.71717929482371 28.02200550137534 20.710177544386095 65-69 22.779555911182236 27.675535107021403 28.165633126625323 21.379275855171034 70-74 21.980495123780948 27.85696424106027 28.687171792948234 21.47536884221055 75-79 22.67566891722931 28.322080520130033 27.906976744186046 21.09527381845461 80-84 23.140785196299074 28.657164291072768 28.322080520130033 19.879969992498125 85-89 22.43060765191298 28.232058014503625 27.926981745436358 21.410352588147035 90-94 23.170792698174544 28.35708927231808 27.196799199799948 21.275318829707427 95-99 22.78569642410603 28.207051762940733 27.94698674668667 21.060265066266567 100-104 23.250812703175793 28.1470367591898 28.262065516379092 20.340085021255312 105-109 22.625656414103528 28.402100525131285 28.092023005751436 20.880220055013755 110-114 23.110777694423607 28.632158039509875 27.981995498874717 20.275068767191797 115-119 23.15078769692423 28.637159289822456 28.042010502625658 20.170042510627656 120-124 22.80570142535634 28.18704676169042 27.701925481370342 21.305326331582897 125-129 23.55588897224306 28.132033008252062 28.012003000750184 20.300075018754686 130-134 24.8186865402891 27.489621367478616 27.769719401790628 19.921972690441656 135-139 24.692407722316695 27.9183755126538 27.608282484745423 19.780934280284086 140-144 24.696174043510876 27.85696424106027 28.02200550137534 19.424856214053513 145-149 25.52638159539885 27.516879219804952 27.286821705426355 19.669917479369843 150-151 26.08478179317244 27.547830436413655 26.7725397023884 19.59484806802551 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 1.0 5 1.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.5 16 1.0 17 0.5 18 1.5 19 2.5 20 1.5 21 0.5 22 4.0 23 4.5 24 4.0 25 7.0 26 5.5 27 9.0 28 11.5 29 13.0 30 21.5 31 33.0 32 39.5 33 50.5 34 55.5 35 59.0 36 80.5 37 129.0 38 176.0 39 193.5 40 205.5 41 215.5 42 249.0 43 261.5 44 257.5 45 275.0 46 262.5 47 240.0 48 232.0 49 190.0 50 138.5 51 123.5 52 100.5 53 76.0 54 60.5 55 41.5 56 39.5 57 31.0 58 26.0 59 22.0 60 9.0 61 6.5 62 6.0 63 5.5 64 2.5 65 0.5 66 1.0 67 1.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.5 73 0.5 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.5 85 0.5 86 0.5 87 0.5 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.5 94 1.5 95 1.0 96 0.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.02 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.034999999999999996 135-139 0.03 140-144 0.025 145-149 0.025 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.475 #Duplication Level Percentage of deduplicated Percentage of total 1 71.78841309823679 49.875 2 18.45987765383231 25.650000000000002 3 5.973371716444764 12.45 4 2.374955019791292 6.6000000000000005 5 0.7556675062972292 2.625 6 0.5037783375314862 2.1 7 0.1439366678661389 0.7000000000000001 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATGACCTACTGTCAATGTTGGTTGCTGGCCATGAGACAACTGGTTCAGT 7 0.17500000000000002 No Hit TGGCCACTGCTTTGGTCATAAAAACTCGTCTTCTCTCTGCACCACGCCTC 7 0.17500000000000002 No Hit GTGGTTGTGAGCTCAAGAATACTACTTGGGCAATTGGAGTTGCTGTTTAT 7 0.17500000000000002 No Hit TTTGGGGTTAGTGGAGGGAGTGTTGGTATGTGTGCTAGTCATGAAGCTGA 7 0.17500000000000002 No Hit GGAAGGAAGAAGGTGCCTGACCAGTTGCAAACTGCAGATGGCTGGGCCAA 6 0.15 No Hit CTTTATTTCACTGCTTCAGCACCAAAATCCATCTGGGGTCTTTTATCTTC 6 0.15 No Hit AGAAAATGCAGATTGGTGACTGGCGGTACTGATAACCATTTGGTGCTGTG 6 0.15 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 6 0.15 No Hit AAGACATCAAAAAGGATGAAAGCAAATAAAAAGTGTTTGTAAGAAAATGG 6 0.15 No Hit CCCCCAAGGAACAGATATTTGAAATGCCGACTGGGGGTGCTGCCATCATG 6 0.15 No Hit GGTGGAATCACTATGTACCAGACAGACTGGAGCATTTCCGCCTTGCAGGT 6 0.15 No Hit GTCTGAGGTACAGATATGGGCTGTTCAAGCAGCGGATCACCAAAGAGGGC 6 0.15 No Hit CCCCGTTTTACCCCTCCCTCTCCTCTTTTTATCCCAATATTACCCTAAAA 6 0.15 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 6 0.15 No Hit AGACCGCCCAAAGTTCTTGGGTCCATTCTCTGAGCAAACCCCATCATACC 6 0.15 No Hit AGAAAGCTTTGCAACAAAGAGATATGGCTTCCCTGGCAGCATCCAGAGCA 6 0.15 No Hit CCTGTCTGCCTATCAGCTTCCACAAAGCCATTTTCTTGTCGTAATCCATA 6 0.15 No Hit GTTCTGAGAAGAAGACTTGCCATAGTTCTCCAGTGAAGTTGCTTACACGA 6 0.15 No Hit CTCCATCTTCCTCTGAAGCCCTAACCATGGCTTCACGCAGGCTTTTATCT 5 0.125 No Hit CATGAAAACCCTTGTTCTAAAGGTTCCTTCAAGCCAGAGACATGTCTATT 5 0.125 No Hit AGATGATGAAGAGGGAAGTGGGGAGGAAGATGACGACGACGAGTAGGATA 5 0.125 No Hit CAAAGGCCGAGTTCCTTGGTGGAGTCTTCCTTACGTCTATTTCGTTGTCT 5 0.125 No Hit TATTGATACAGCAATTGATGTGGCTTCTTTGGATTTTGGGTCTGAATTGA 5 0.125 No Hit GTTGTCAGGTGGCGACAGTCAGATGAATACACTGATTTGGTATAGCTGGC 5 0.125 No Hit CCTACAACGACGCTTCTCTCCCCTTCCATGGCGAGAACTCTCCTTTCATC 5 0.125 No Hit GAACTCTTTGGTGGTAATCGCCTCGCCGCCTTCCCTACTGCTTGTGCTCT 5 0.125 No Hit CTTGTAGAAATGATAAATCTACATTACATGTCGACCTTGTGTTCGCTTGC 5 0.125 No Hit CGCGTCACTCATCGGTCAGTCGTGAAGAAGTCTCATCCGTGTCATCTAAT 5 0.125 No Hit AGGATACTTCTTTCACTAGGATGCCACAGCTGTCCTCCTTTGTGTGCATT 5 0.125 No Hit ATTCGATCCTGTTGAGAAGATTAAATCCGGCTTCATTCACTTCAAGAAGG 5 0.125 No Hit CTTCAAGTGTTTCTTGATGGAGGGTTGAACCAGCAAAGAATGGGGATATG 5 0.125 No Hit ACCACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCC 5 0.125 No Hit CTGCACTGCACTGAATCAGTGGAAAATTTCCAAGCTCCTCCTACTTCTCT 5 0.125 No Hit CGATGACAGCAGTGATTTGTTTAGTACTGGCAGTGAACTAGTTGTGAATT 5 0.125 No Hit GTTGATACAGATTCCTCAATTTTGCATCAGGTACAGGAAAGCATTCAGGT 5 0.125 No Hit CCTCATCCTCCACGTTCTGTCATATCATACACCTTATAGCCATGGAATTT 5 0.125 No Hit TCGAAACAAAGCCTGTCTTACTTGGATGGCAGCCTTCCAGGTGACTTCGG 5 0.125 No Hit GCTGCTTCTCCAATGGCCAGCCAGCTCAAGAGCAGCTTTGCCTCATCTTT 5 0.125 No Hit CGGGTTCTAAGAGAATGGCCACCAATACATTGATGAGCTGCGGCATTGCC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.275 0.0 0.0 0.0 0.0 84-85 0.2875 0.0 0.0 0.0 0.0 86-87 0.4125 0.0 0.0 0.0 0.0 88-89 0.625 0.0 0.0 0.0 0.0 90-91 0.7875 0.0 0.0 0.0 0.0 92-93 1.0125000000000002 0.0 0.0 0.0 0.0 94-95 1.1375 0.0 0.0 0.0 0.0 96-97 1.35 0.0 0.0 0.0 0.0 98-99 1.4249999999999998 0.0 0.0 0.0 0.0 100-101 1.4874999999999998 0.0 0.0 0.0 0.0 102-103 1.55 0.0 0.0 0.0 0.0 104-105 1.65 0.0 0.0 0.0 0.0 106-107 1.7875 0.0 0.0 0.0 0.0 108-109 1.9874999999999998 0.0 0.0 0.0 0.0 110-111 2.3625 0.0 0.0 0.0 0.0 112-113 2.6500000000000004 0.0 0.0 0.0 0.0 114-115 2.8 0.0 0.0 0.0 0.0 116-117 2.9875 0.0 0.0 0.0 0.0 118-119 3.4124999999999996 0.0 0.0 0.0 0.0 120-121 3.7125 0.0 0.0 0.0 0.0 122-123 3.925 0.0 0.0 0.0 0.0 124-125 4.275 0.0 0.0 0.0 0.0 126-127 4.7625 0.0 0.0 0.0 0.0 128-129 5.2125 0.0 0.0 0.0 0.0 130-131 5.762499999999999 0.0 0.0 0.0 0.0 132-133 6.1875 0.0 0.0 0.0 0.0 134-135 6.6625 0.0 0.0 0.0 0.0 136-137 7.225 0.0 0.0 0.0 0.0 138-139 7.75 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra Read 981839 spots for SRR13695492.sra Written 981839 spots for SRR13695492.sra SRR ids: ['SRR13695492.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_c240wn1y SRR13695492.sra spots: 19636780 blocks: [[1, 981839], [981840, 1963678], [1963679, 2945517], [2945518, 3927356], [3927357, 4909195], [4909196, 5891034], [5891035, 6872873], [6872874, 7854712], [7854713, 8836551], [8836552, 9818390], [9818391, 10800229], [10800230, 11782068], [11782069, 12763907], [12763908, 13745746], [13745747, 14727585], [14727586, 15709424], [15709425, 16691263], [16691264, 17673102], [17673103, 18654941], [18654942, 19636780]] SRR13695492 file size 6651736 SRR13695492 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695492 SRR13695492_1.fastq SRR13695492_2.fastq Input file: SRR13695492_1.fastq Paired file: SRR13695492_2.fastq trimmed: SRR13695492-trimmed-pair1.fastq, SRR13695492-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 07:38:06 2025 >> started Wed Feb 12 07:38:28 2025 >> done (22.075s) 19636780 read pairs processed; of these: 126 ( 0.00%) short read pairs filtered out after trimming by size control 1574 ( 0.01%) empty read pairs filtered out after trimming by size control 19635080 (99.99%) read pairs available; of these: 1947090 ( 9.92%) trimmed read pairs available after processing 17687990 (90.08%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 20 3 0.00% 21 4 0.00% 22 6 0.00% 23 1 0.00% 24 0 0.00% 25 3 0.00% 26 1 0.00% 27 2 0.00% 28 2 0.00% 29 3 0.00% 30 9 0.00% 31 3 0.00% 32 5 0.00% 33 1 0.00% 34 7 0.00% 35 5 0.00% 36 10 0.00% 37 0 0.00% 38 16 0.00% 39 7 0.00% 40 11 0.00% 41 9 0.00% 42 14 0.00% 43 28 0.00% 44 28 0.00% 45 17 0.00% 46 16 0.00% 47 30 0.00% 48 24 0.00% 49 43 0.00% 50 51 0.00% 51 50 0.00% 52 67 0.00% 53 89 0.00% 54 107 0.00% 55 102 0.00% 56 97 0.00% 57 118 0.00% 58 138 0.00% 59 171 0.00% 60 229 0.00% 61 219 0.00% 62 293 0.00% 63 363 0.00% 64 460 0.00% 65 406 0.00% 66 441 0.00% 67 634 0.00% 68 671 0.00% 69 721 0.00% 70 895 0.00% 71 1138 0.01% 72 1165 0.01% 73 1419 0.01% 74 1669 0.01% 75 1641 0.01% 76 1974 0.01% 77 2228 0.01% 78 2413 0.01% 79 2765 0.01% 80 3105 0.02% 81 3512 0.02% 82 3734 0.02% 83 4058 0.02% 84 4748 0.02% 85 5292 0.03% 86 5488 0.03% 87 5942 0.03% 88 6460 0.03% 89 6923 0.04% 90 7183 0.04% 91 7849 0.04% 92 8339 0.04% 93 8942 0.05% 94 9725 0.05% 95 10632 0.05% 96 11163 0.06% 97 11742 0.06% 98 12150 0.06% 99 12640 0.06% 100 13461 0.07% 101 13820 0.07% 102 14946 0.08% 103 15415 0.08% 104 16442 0.08% 105 17262 0.09% 106 18368 0.09% 107 18675 0.10% 108 19318 0.10% 109 20257 0.10% 110 20617 0.11% 111 21399 0.11% 112 22344 0.11% 113 23262 0.12% 114 24423 0.12% 115 25501 0.13% 116 26550 0.14% 117 27165 0.14% 118 27765 0.14% 119 29315 0.15% 120 29649 0.15% 121 30535 0.16% 122 31655 0.16% 123 32252 0.16% 124 33491 0.17% 125 34295 0.17% 126 35504 0.18% 127 36311 0.18% 128 37266 0.19% 129 38278 0.19% 130 39550 0.20% 131 40078 0.20% 132 40523 0.21% 133 41929 0.21% 134 42164 0.21% 135 43561 0.22% 136 44875 0.23% 137 45979 0.23% 138 46104 0.23% 139 48488 0.25% 140 48671 0.25% 141 49158 0.25% 142 50780 0.26% 143 50788 0.26% 144 52168 0.27% 145 53229 0.27% 146 53429 0.27% 147 56052 0.29% 148 56291 0.29% 149 56867 0.29% 150 58196 0.30% 151 17687990 90.08% 19635080 reads passed initial QC criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=30 prefix-density=0.35 prefix-fanout=2.0 sequence=GTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=590.77 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=18.4 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTC criterion=sequence-density sequence-density=0.43 sequence-density-rank=1 fanout-score=2.11 fanout-score-rank=30 prefix-density=0.44 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=86.22 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=9.1 sequence=AAACCCAAAGCTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAATGGCCCTCTCCTCCCCTTCGCTAGCCGGAAAGGCGGTGAAGCTCAACCCCTCCTCCTCTGAGATCATGGGCAATGGCCGTGTCTCCATGAGGAAAACCACCAAGCCTGTTCCCTCCGGGAGCCCATGGTACGGACCAGACCGTGTTAAATACTTGGGCCCGTTCTCTGGTGAGCCCCCATCCTACTTGACTGGTGAGTTCCCTGGTGACTACGGCTGGGACACTGCTGG SRR13695492 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 07:39:11 Started mapping on | Feb 12 07:39:11 Finished on | Feb 12 07:41:41 Mapping speed, Million of reads per hour | 471.24 Number of input reads | 19635080 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 18202177 Uniquely mapped reads % | 92.70% Average mapped length | 295.65 Number of splices: Total | 17821246 Number of splices: Annotated (sjdb) | 17402098 Number of splices: GT/AG | 17467709 Number of splices: GC/AG | 273014 Number of splices: AT/AC | 10375 Number of splices: Non-canonical | 70148 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 3.03 Insertion rate per base | 0.02% Insertion average length | 2.35 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 427301 % of reads mapped to multiple loci | 2.18% Number of reads mapped to too many loci | 27384 % of reads mapped to too many loci | 0.14% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.90% % of reads unmapped: other | 0.09% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1005825 1005825 1005825 N_multimapping 427301 427301 427301 N_noFeature 741056 17735994 1051942 N_ambiguous 265769 1910 109055 UnstrandedReadsAssigned:17195352 PositiveStrandReadsAssigned:464273 NegativeStrandReadsAssigned:17041180 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695492 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695492-trimmed-pair1.fastq SRR13695492-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,635,080 reads, 17,020,853 reads pseudoaligned [quant] estimated average fragment length: 258.047 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,131 rounds 52401 SRR13695492.ke.tsv 34699 SRR13695492.se.tsv 87100 total ==> SRR13695492.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1760.95 657 20.9811 Potri.005G024800.1.v4.1 1035 777.953 190 13.7344 Potri.004G059700.1.v4.1 961 704.089 0 0 Potri.007G009000.2.v4.1 1416 1158.95 0 0 Potri.003G141000.2.v4.1 2943 2685.95 926 19.3876 Potri.016G087400.1.v4.1 270 82.7939 733.113 497.947 Potri.015G069301.1.v4.1 564 318.909 0 0 Potri.010G195200.1.v4.1 1773 1515.95 274 10.1642 Potri.012G127500.1.v4.1 977 720.013 17 1.32776 ==> SRR13695492.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 157 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 282 Potri.001G212900.v4.1 6 Potri.001G182400.v4.1 2 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 8 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695492 completed mapping pipeline successfully