Starting /dee2/code/volunteer_pipeline.sh SRR13695493 current disk space = 3049946390528 free memory = 1581791704 SRR13695493 SRAfilesize db3694ae1c73f5007b8398d24e6da55b SRR13695493.sra SRR13695493.sra file validated SRR13695493 is paired end SRR13695493 is conventional basespace SRR13695493 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695493_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.547 37.0 37.0 37.0 37.0 37.0 2 36.21475 37.0 37.0 37.0 37.0 37.0 3 36.59 37.0 37.0 37.0 37.0 37.0 4 36.5415 37.0 37.0 37.0 37.0 37.0 5 36.5425 37.0 37.0 37.0 37.0 37.0 6 36.5905 37.0 37.0 37.0 37.0 37.0 7 36.4315 37.0 37.0 37.0 37.0 37.0 8 36.553 37.0 37.0 37.0 37.0 37.0 9 36.5495 37.0 37.0 37.0 37.0 37.0 10-14 36.5435 37.0 37.0 37.0 37.0 37.0 15-19 36.5003 37.0 37.0 37.0 37.0 37.0 20-24 36.516 37.0 37.0 37.0 37.0 37.0 25-29 36.4702 37.0 37.0 37.0 37.0 37.0 30-34 36.440999999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.4222 37.0 37.0 37.0 37.0 37.0 40-44 36.410199999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.3523 37.0 37.0 37.0 37.0 37.0 50-54 36.3538 37.0 37.0 37.0 37.0 37.0 55-59 36.3282 37.0 37.0 37.0 37.0 37.0 60-64 36.322900000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.278600000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.3175 37.0 37.0 37.0 37.0 37.0 75-79 36.342999999999996 37.0 37.0 37.0 37.0 37.0 80-84 36.245900000000006 37.0 37.0 37.0 37.0 37.0 85-89 36.2765 37.0 37.0 37.0 37.0 37.0 90-94 36.23819999999999 37.0 37.0 37.0 37.0 37.0 95-99 36.120599999999996 37.0 37.0 37.0 37.0 37.0 100-104 36.232800000000005 37.0 37.0 37.0 37.0 37.0 105-109 36.188199999999995 37.0 37.0 37.0 37.0 37.0 110-114 36.1372 37.0 37.0 37.0 37.0 37.0 115-119 36.1536 37.0 37.0 37.0 37.0 37.0 120-124 36.099000000000004 37.0 37.0 37.0 37.0 37.0 125-129 36.0254 37.0 37.0 37.0 37.0 37.0 130-134 36.0106 37.0 37.0 37.0 37.0 37.0 135-139 35.896 37.0 37.0 37.0 37.0 37.0 140-144 35.797 37.0 37.0 37.0 37.0 37.0 145-149 35.642399999999995 37.0 37.0 37.0 37.0 37.0 150-151 35.524 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 2.0 25 1.0 26 2.0 27 7.0 28 15.0 29 15.0 30 26.0 31 37.0 32 53.0 33 69.0 34 160.0 35 280.0 36 2978.0 37 355.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.849999999999998 11.95 13.175 45.025 2 18.87742260256733 16.31009312861817 33.82834130380065 30.984142965013845 3 17.9 15.35 25.85 40.9 4 21.0 23.35 22.5 33.15 5 23.625 29.599999999999998 24.0 22.775000000000002 6 22.05 33.800000000000004 24.125 20.025000000000002 7 15.475 28.975 37.974999999999994 17.575 8 18.6 27.85 31.900000000000002 21.65 9 18.5 24.9 33.4 23.200000000000003 10-14 19.905 29.459999999999997 27.46 23.175 15-19 20.225 28.84 27.175 23.76 20-24 19.52 29.225 27.544999999999998 23.71 25-29 19.725 28.645 27.534999999999997 24.095 30-34 19.77 28.93 26.85 24.45 35-39 20.3 28.060000000000002 27.900000000000002 23.74 40-44 19.38 29.815 26.700000000000003 24.104999999999997 45-49 20.105 28.73 27.389999999999997 23.775 50-54 20.06 28.865000000000002 27.37 23.705000000000002 55-59 19.7 28.615000000000002 27.334999999999997 24.349999999999998 60-64 20.48 28.255000000000003 27.150000000000002 24.115000000000002 65-69 20.369999999999997 28.494999999999997 27.689999999999998 23.445 70-74 20.465 27.575 27.055 24.905 75-79 20.990000000000002 28.21 27.215 23.585 80-84 20.24 28.225 27.975 23.56 85-89 20.355 28.34 27.305 24.0 90-94 20.825 27.525 26.939999999999998 24.709999999999997 95-99 20.925 28.035 27.015 24.025 100-104 20.395 28.83 26.450000000000003 24.325 105-109 20.580000000000002 28.720000000000002 26.790000000000003 23.91 110-114 20.669999999999998 29.189999999999998 26.8 23.34 115-119 20.979999999999997 28.51 26.71 23.799999999999997 120-124 21.38 28.599999999999998 25.995 24.025 125-129 21.58 28.025 26.51 23.885 130-134 21.215 27.865000000000002 26.985 23.935000000000002 135-139 21.67 27.815 26.450000000000003 24.065 140-144 21.915000000000003 27.750000000000004 26.52 23.815 145-149 21.12 28.48 26.005 24.395 150-151 22.0625 27.3375 26.5375 24.0625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.0 23 1.0 24 1.0 25 6.5 26 10.5 27 9.5 28 7.5 29 8.5 30 13.5 31 18.5 32 30.5 33 41.5 34 57.0 35 72.0 36 81.0 37 103.5 38 127.0 39 150.0 40 180.0 41 199.5 42 214.5 43 241.0 44 260.5 45 251.5 46 242.0 47 248.0 48 250.5 49 224.0 50 190.5 51 162.0 52 134.5 53 105.5 54 80.5 55 74.0 56 62.5 57 42.0 58 28.5 59 20.5 60 11.5 61 9.0 62 6.5 63 2.5 64 1.5 65 2.5 66 5.0 67 5.0 68 3.0 69 1.5 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.675 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.0 #Duplication Level Percentage of deduplicated Percentage of total 1 71.85714285714285 50.3 2 18.464285714285715 25.85 3 6.392857142857143 13.425 4 2.107142857142857 5.8999999999999995 5 0.8214285714285714 2.875 6 0.17857142857142858 0.75 7 0.14285714285714285 0.7000000000000001 8 0.03571428571428571 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCCACAGGACTGAGTACAGGCCTATGACTATCAAGACTGAACCAACAAT 8 0.2 No Hit GTGTACCTTGTGTGACCTTCACGAATAGCATTTATACCAGCCTCTGCTAT 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGGTCAATCGCGTAT 7 0.17500000000000002 TruSeq Adapter, Index 1 (97% over 36bp) CTCCAGTTTTCCTGAATGTGTGATTTAGGGTTGGGCTTGAGAGCATGAAC 7 0.17500000000000002 No Hit ATCAGGATGTTTCCCAGCGAAATCCACTGGTCTATGAAACAGCAAATGGG 7 0.17500000000000002 No Hit GCCAATTCTCCCCTATTGAAACAGCAAAGAAACAAACAAACGGTAACAAA 6 0.15 No Hit GCAATATTTGAATGTGGAGAAAGATCATTAGCAATGCCATAGAAATATTC 6 0.15 No Hit TTTGGGATTTTCCCTGTAAACTCCATGTTTGCAGCAAGAAGTAATCCAAG 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACGGTCAATCTCGTAT 6 0.15 TruSeq Adapter, Index 1 (97% over 36bp) GCCTTCTCTTGCATGCTGGTAAAAACTCTCACCAAACCACCACCAAAATT 6 0.15 No Hit CACAGAAGTTGCGGTGTGGAAATGGCTGAGCCCCAGGGCATAGAAAGCTC 5 0.125 No Hit CAGCCATGTTACTAAATGCCTTTCCACCTGCCCTGCTTAATGCTGCAAAT 5 0.125 No Hit CTTGACAAACCTCAGTACCAAACTTGTCTTCCCGGTCCCCATGTCCCCGA 5 0.125 No Hit TTTCGGAAAATGTCGTAACAAGGCATGTAGATTCCAACAGTAGGGACAGC 5 0.125 No Hit GCTCTTTCAGGACATTCATGAAACACATATGAAACTGAAACAAGATCGAA 5 0.125 No Hit GTGCTGCTAATTGTTTAATGGTGAAAAGGAAAGGCACACGCTCGCCACCA 5 0.125 No Hit CCTGTACTTTCTTCACTGTCACTACCATTACCACTTCTAAAGGCAGACAT 5 0.125 No Hit CTCTTGTCTCCAGCTTGTGCCTTGCAAATGTAAAGCTTACCATCTTTCAC 5 0.125 No Hit CCTCAAATGCCGAGAAAGCATCGGCAGCTAATACCTCTGCCCACTGGTAG 5 0.125 No Hit CTTCAACAGACTTTGCCGCATCAACCTTCCTAACTTTCCCCTTTGAGTCA 5 0.125 No Hit TGCCATATTTTTTTTTATTAAAAAAAACACACAATTACAGCAAATGAATA 5 0.125 No Hit CTAAAGATCAAACCAGTAGCCAAAGGCACGTCAATAATGATCTCCTTCAT 5 0.125 No Hit GAGTTTAATTGCACAAAGCTGAGATATGAAGTTTGACAATGCCATCCAAA 5 0.125 No Hit CATCAGTGTTGTAGATAGGGCTGTAGCCATCCACATTAGCACCATATTTG 5 0.125 No Hit CGAGGAACATAGAGATGAGGATTAAGCAGTAGCTGCAGCCTGTTCTTCAA 5 0.125 No Hit TCGGCCGACAACACATCAGGGGTTCTGTCCGGTGATGAGGATTTACAGAA 5 0.125 No Hit GCTCAGTGCTAATGGCAGAATAATCACTCATCATGCTTGAATTATCAGCA 5 0.125 No Hit GGTAAATATCAACAGTAGCGTTGACAGTTGGCAAAAGTTGGAGGTAAAAA 5 0.125 No Hit ATCCAGTTGGAGGCCACACCTGCATGCATTGAACTCTTCCGCCATTGCTT 5 0.125 No Hit ACAGCAAATACTATATTAGACAAACATGGAGTGACCAGACTTCTTCTTTT 5 0.125 No Hit CTGCATCCTTCTCTTATAATCAACACAAATGGAGAGCTACAATTGGAACC 5 0.125 No Hit CCCTCTTCTTTTCAAAACACGAATAAAAGTATCTTATTTTTCTCGTAGAG 5 0.125 No Hit TCTCAGCACCCACAGCTGTGCCTGTGCTGTACCCGGGAGCCACCATGTTA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.21250000000000002 0.0 0.0 0.0 0.0 78-79 0.30000000000000004 0.0 0.0 0.0 0.0 80-81 0.3875 0.0 0.0 0.0 0.0 82-83 0.5375000000000001 0.0 0.0 0.0 0.0 84-85 0.6125 0.0 0.0 0.0 0.0 86-87 0.7625 0.0 0.0 0.0 0.0 88-89 0.875 0.0 0.0 0.0 0.0 90-91 0.9875 0.0 0.0 0.0 0.0 92-93 1.1625 0.0 0.0 0.0 0.0 94-95 1.2999999999999998 0.0 0.0 0.0 0.0 96-97 1.425 0.0 0.0 0.0 0.0 98-99 1.725 0.0 0.0 0.0 0.0 100-101 2.0625 0.0 0.0 0.0 0.0 102-103 2.2874999999999996 0.0 0.0 0.0 0.0 104-105 2.675 0.0 0.0 0.0 0.0 106-107 2.8875 0.0 0.0 0.0 0.0 108-109 3.125 0.0 0.0 0.0 0.0 110-111 3.4125 0.0 0.0 0.0 0.0 112-113 3.7750000000000004 0.0 0.0 0.0 0.0 114-115 4.05 0.0 0.0 0.0 0.0 116-117 4.324999999999999 0.0 0.0 0.0 0.0 118-119 4.625 0.0 0.0 0.0 0.0 120-121 5.0375 0.0 0.0 0.0 0.0 122-123 5.5375 0.0 0.0 0.0 0.0 124-125 6.074999999999999 0.0 0.0 0.0 0.0 126-127 6.5875 0.0 0.0 0.0 0.0 128-129 7.1 0.0 0.0 0.0 0.0 130-131 7.5 0.0 0.0 0.0 0.0 132-133 7.85 0.0 0.0 0.0 0.0 134-135 8.425 0.0 0.0 0.0 0.0 136-137 9.024999999999999 0.0 0.0 0.0 0.0 138-139 9.625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13695493 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695493_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2625 37.0 37.0 37.0 37.0 37.0 2 36.204 37.0 37.0 37.0 37.0 37.0 3 36.1655 37.0 37.0 37.0 37.0 37.0 4 36.2385 37.0 37.0 37.0 37.0 37.0 5 36.2455 37.0 37.0 37.0 37.0 37.0 6 36.28 37.0 37.0 37.0 37.0 37.0 7 36.321 37.0 37.0 37.0 37.0 37.0 8 36.3465 37.0 37.0 37.0 37.0 37.0 9 36.3145 37.0 37.0 37.0 37.0 37.0 10-14 36.294 37.0 37.0 37.0 37.0 37.0 15-19 36.2736 37.0 37.0 37.0 37.0 37.0 20-24 36.30495 37.0 37.0 37.0 37.0 37.0 25-29 36.17115 37.0 37.0 37.0 37.0 37.0 30-34 36.177049999999994 37.0 37.0 37.0 37.0 37.0 35-39 36.125150000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.117050000000006 37.0 37.0 37.0 37.0 37.0 45-49 36.09739999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.099149999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.06695 37.0 37.0 37.0 37.0 37.0 60-64 36.00885 37.0 37.0 37.0 37.0 37.0 65-69 35.967 37.0 37.0 37.0 37.0 37.0 70-74 35.996249999999996 37.0 37.0 37.0 37.0 37.0 75-79 35.965700000000005 37.0 37.0 37.0 37.0 37.0 80-84 35.944500000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.914649999999995 37.0 37.0 37.0 37.0 37.0 90-94 35.926300000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.95285 37.0 37.0 37.0 37.0 37.0 100-104 35.885149999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.85615 37.0 37.0 37.0 37.0 37.0 110-114 35.79495 37.0 37.0 37.0 37.0 37.0 115-119 35.79445 37.0 37.0 37.0 37.0 37.0 120-124 35.69065 37.0 37.0 37.0 37.0 37.0 125-129 35.7164 37.0 37.0 37.0 37.0 37.0 130-134 35.59685 37.0 37.0 37.0 37.0 37.0 135-139 35.549800000000005 37.0 37.0 37.0 37.0 37.0 140-144 35.437250000000006 37.0 37.0 37.0 37.0 37.0 145-149 35.273250000000004 37.0 37.0 37.0 34.6 37.0 150-151 34.891375 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 1.0 15 1.0 16 2.0 17 2.0 18 1.0 19 0.0 20 3.0 21 1.0 22 2.0 23 2.0 24 4.0 25 6.0 26 7.0 27 15.0 28 13.0 29 16.0 30 26.0 31 44.0 32 38.0 33 108.0 34 205.0 35 543.0 36 2719.0 37 240.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 30.055193176116408 18.840943301555445 19.744104365278474 31.359759157049673 2 29.875 23.05 27.625 19.45 3 21.099999999999998 26.674999999999997 31.1 21.125 4 23.799999999999997 31.974999999999998 24.9 19.325 5 26.35 32.875 23.599999999999998 17.175 6 22.525000000000002 37.4 22.025 18.05 7 20.65 21.85 38.824999999999996 18.675 8 24.175 24.075 28.625 23.125 9 23.275000000000002 24.5 31.225 21.0 10-14 24.07 28.595 25.715 21.62 15-19 23.45 27.57 27.49 21.490000000000002 20-24 23.986199309965496 28.371418570928547 26.786339316965847 20.856042802140106 25-29 23.460865216304075 27.526881720430108 27.97199299824956 21.040260065016252 30-34 23.563534530179528 28.20423063459519 27.03905585837876 21.19317897684653 35-39 23.741187059352967 28.21641082054103 27.051352567628385 20.991049552477623 40-44 24.158623793569035 27.13407011051658 28.264239635945394 20.443066459968996 45-49 23.74737473747375 27.08770877087709 27.94279427942794 21.222122212221223 50-54 24.591229561478073 27.651382569128458 26.82134106705335 20.936046802340115 55-59 24.336084021005252 27.291822955738937 27.346836709177296 21.02525631407852 60-64 24.031201560078003 28.15640782039102 27.546377318865943 20.266013300665033 65-69 23.905 27.595 27.3 21.2 70-74 23.570892723180794 27.936984246061513 27.751937984496124 20.740185046261566 75-79 24.0248049609922 27.335467093418686 28.280656131226245 20.35907181436287 80-84 24.09 27.639999999999997 26.56 21.709999999999997 85-89 24.77119279819955 26.881720430107524 27.35183795948987 20.99524881220305 90-94 23.697369736973698 28.212821282128214 26.72267226722672 21.367136713671368 95-99 24.011200560028 26.94634731736587 27.956397819890995 21.086054302715134 100-104 24.516129032258064 28.62215553888472 26.971742935733932 19.88997249312328 105-109 24.72118029507377 27.746936734183546 27.051762940735184 20.4801200300075 110-114 25.501275063753187 28.30641532076604 26.446322316115804 19.745987299364966 115-119 24.971242810702677 27.4368592148037 27.121780445111277 20.470117529382346 120-124 24.991247811952988 27.73693423355839 27.561890472618156 19.70992748187047 125-129 25.50510102020404 27.125425085017003 27.540508101620325 19.82896579315863 130-134 25.388886110138547 27.544640624218474 27.454609113189615 19.61186415245336 135-139 24.947484245273582 27.35820746223867 27.493247974392315 20.20106031809543 140-144 25.891294564728234 27.326366318315916 26.70133506675334 20.081004050202512 145-149 25.881470367591895 27.721930482620653 26.76669167291823 19.629907476869217 150-151 28.010503938977116 27.49781167937977 24.796798799549833 19.694885582093285 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.5 13 0.5 14 0.5 15 0.5 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.5 22 1.5 23 1.5 24 1.0 25 1.5 26 2.0 27 3.0 28 4.0 29 5.5 30 9.5 31 16.5 32 25.5 33 27.0 34 34.5 35 55.0 36 66.5 37 77.5 38 116.5 39 157.0 40 170.0 41 195.0 42 240.5 43 264.0 44 280.0 45 304.0 46 297.0 47 263.0 48 229.5 49 207.0 50 174.0 51 158.0 52 148.0 53 118.0 54 92.5 55 64.5 56 47.5 57 37.5 58 29.0 59 16.0 60 9.5 61 11.0 62 4.5 63 3.5 64 4.5 65 1.5 66 0.5 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 1.0 87 1.5 88 0.5 89 0.5 90 0.5 91 0.5 92 1.0 93 2.0 94 2.0 95 1.0 96 1.0 97 1.0 98 1.5 99 1.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.015 35-39 0.005 40-44 0.015 45-49 0.01 50-54 0.005 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.02 80-84 0.0 85-89 0.025 90-94 0.01 95-99 0.005 100-104 0.025 105-109 0.025 110-114 0.005 115-119 0.025 120-124 0.025 125-129 0.02 130-134 0.034999999999999996 135-139 0.03 140-144 0.005 145-149 0.025 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.65 #Duplication Level Percentage of deduplicated Percentage of total 1 72.50530785562633 51.225 2 18.117480537862704 25.6 3 6.334041047416844 13.425 4 1.9815994338287333 5.6000000000000005 5 0.743099787685775 2.625 6 0.17692852087756544 0.75 7 0.07077140835102619 0.35000000000000003 8 0.035385704175513094 0.2 9 0.035385704175513094 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCCTC 9 0.22499999999999998 No Hit GTTTGGGCAATTGGCTGGGACATGAACCTACTTGCTGCTGCCTATGCTGG 8 0.2 No Hit ACCTCTCGGAACCAATCCGGGTTCCCACTCTCAACCCTCCGGATCTGTCT 7 0.17500000000000002 No Hit GGCAGTCCTGAAACTCTAAGAGACCCCAGAGGTTTTGCTGTGAAATTCTA 7 0.17500000000000002 No Hit GTGATACTTATGGGTCAACTGATTTTGATGTCCTTGAAAATGTTAGAGAT 6 0.15 No Hit ACCTGTGCGAACGACTACATGGATCAGGGCATTGCTTTATTGTGCTCTAG 6 0.15 No Hit AGAAGGTGTAGGCTATGAGTTGCTTCTTCATGCAATTGATACATTCAATG 6 0.15 No Hit GGAAGATCTTCTCAAGGCTGAAGAGATAGCTGCAAAATATCGTGCCACTG 6 0.15 No Hit TATTGAGCAAGATGATTCCAATACAATCTCTCTGGCAGTTAATGATTACT 6 0.15 No Hit TTTGAAGGTTACGAAAAAAGGCTTGAAATATCTTTCCTAGAGCCTGGCTT 5 0.125 No Hit CGCTTATCTCCATTAGGGAAGAAATTGCAGAGATTGAGAAAGGCAAAGCT 5 0.125 No Hit TAAATCTCTCTCTCTCTCTCTCTCTCTCTCTCTGAGATTTCTTCATCGAT 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 5 0.125 No Hit CACGAATGTCTTTAGGTTTGGAAAACTCCTCCATGATCCAGTAGTAGATT 5 0.125 No Hit GGGGTGAGTTTATCACACCAGGGATTTTGTTCTTGTACATTGCTGGATGG 5 0.125 No Hit ATTGAAGCTAATTTCGCTCCTGCAATAAAGGGCTCAGGTGAATGTGGGGT 5 0.125 No Hit GCAAGCACCAACTTATCACAGCCACTGTGAAAGATGGTAAGCTTTACATT 5 0.125 No Hit GGAAAGTGTCCCTAATCAGGGCTGTGGGCTACATGGTGGCTCAATGCTTG 5 0.125 No Hit GCTAGGTAGCTTCAATGGCAAAACCAATTTCAATTGAAGTCTACAATCCA 5 0.125 No Hit AGATTATTATGTCTTTGTGCTGCTAAGTTATTTGTCTTCTTTCTACTTTG 5 0.125 No Hit CCCGTGACACTGCGGAGAAAATCAAGTATGTAGTTGAATCCGTTGACGAC 5 0.125 No Hit GCTCCATAAAAATTATATTCCAGGTGGGCTAGAATCTGTCTTTGATATTG 5 0.125 No Hit CCTATGATGAAATCCAGAGCAAGACATACATGGAAGTAAAAGGATCTGGA 5 0.125 No Hit CACGAATTAAAGACTGGTCCAGAAATAGTGAGTTCATCAAACGTTGTTGC 5 0.125 No Hit CTACCATGATTAGAGACATTCTAGATATTGGATGTTCTGTAGGTGTTAGC 5 0.125 No Hit CCTTACGGAACTGGTGGTGGCATGAACCTCAGGGATGGGTTAGATGCATC 5 0.125 No Hit CTCTTCCTTCCACCAAGAACATCCTCCACTGGTGTCGAAAGACACTCTCG 5 0.125 No Hit GGAAACTGTGGCTGCGGCTCTGGATGCAAGTGCGGCAGTGGCTGCAATGG 5 0.125 No Hit TCTGCCATTATCTCTCACAAAAATGATGTCAACTCCTGCTATTCTCCACA 5 0.125 No Hit GAGATGGAGAAGCGCATTTTGAGCAGGAACCAGGGAAGAGAAGATGATAA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.21250000000000002 0.0 0.0 0.0 0.0 78-79 0.30000000000000004 0.0 0.0 0.0 0.0 80-81 0.3875 0.0 0.0 0.0 0.0 82-83 0.5375000000000001 0.0 0.0 0.0 0.0 84-85 0.6125 0.0 0.0 0.0 0.0 86-87 0.7625 0.0 0.0 0.0 0.0 88-89 0.875 0.0 0.0 0.0 0.0 90-91 0.9875 0.0 0.0 0.0 0.0 92-93 1.1625 0.0 0.0 0.0 0.0 94-95 1.2999999999999998 0.0 0.0 0.0 0.0 96-97 1.425 0.0 0.0 0.0 0.0 98-99 1.7374999999999998 0.0 0.0 0.0 0.0 100-101 2.0875000000000004 0.0 0.0 0.0 0.0 102-103 2.3125 0.0 0.0 0.0 0.0 104-105 2.6875 0.0 0.0 0.0 0.0 106-107 2.8875 0.0 0.0 0.0 0.0 108-109 3.125 0.0 0.0 0.0 0.0 110-111 3.4375 0.0 0.0 0.0 0.0 112-113 3.8 0.0 0.0 0.0 0.0 114-115 4.1 0.0 0.0 0.0 0.0 116-117 4.4125 0.0 0.0 0.0 0.0 118-119 4.725 0.0 0.0 0.0 0.0 120-121 5.1375 0.0 0.0 0.0 0.0 122-123 5.6375 0.0 0.0 0.0 0.0 124-125 6.175000000000001 0.0 0.0 0.0 0.0 126-127 6.6875 0.0 0.0 0.0 0.0 128-129 7.2 0.0 0.0 0.0 0.0 130-131 7.575 0.0 0.0 0.0 0.0 132-133 7.925000000000001 0.0 0.0 0.0 0.0 134-135 8.5 0.0 0.0 0.0 0.0 136-137 9.100000000000001 0.0 0.0 0.0 0.0 138-139 9.675 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCT 10 0.006830828 145.0 8 >>END_MODULE Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046951 spots for SRR13695493.sra Written 1046951 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra Read 1046934 spots for SRR13695493.sra Written 1046934 spots for SRR13695493.sra SRR ids: ['SRR13695493.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_2jngowb5 SRR13695493.sra spots: 20938697 blocks: [[1, 1046934], [1046935, 2093868], [2093869, 3140802], [3140803, 4187736], [4187737, 5234670], [5234671, 6281604], [6281605, 7328538], [7328539, 8375472], [8375473, 9422406], [9422407, 10469340], [10469341, 11516274], [11516275, 12563208], [12563209, 13610142], [13610143, 14657076], [14657077, 15704010], [15704011, 16750944], [16750945, 17797878], [17797879, 18844812], [18844813, 19891746], [19891747, 20938697]] SRR13695493 file size 7094184 SRR13695493 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695493 SRR13695493_1.fastq SRR13695493_2.fastq Input file: SRR13695493_1.fastq Paired file: SRR13695493_2.fastq trimmed: SRR13695493-trimmed-pair1.fastq, SRR13695493-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 07:33:38 2025 >> started Wed Feb 12 07:34:01 2025 >> done (22.403s) 20938697 read pairs processed; of these: 131 ( 0.00%) short read pairs filtered out after trimming by size control 53958 ( 0.26%) empty read pairs filtered out after trimming by size control 20884608 (99.74%) read pairs available; of these: 3067772 (14.69%) trimmed read pairs available after processing 17816836 (85.31%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 5 0.00% 21 5 0.00% 22 1 0.00% 23 0 0.00% 24 1 0.00% 25 1 0.00% 26 2 0.00% 27 2 0.00% 28 1 0.00% 29 2 0.00% 30 4 0.00% 31 6 0.00% 32 7 0.00% 33 10 0.00% 34 4 0.00% 35 3 0.00% 36 12 0.00% 37 22 0.00% 38 15 0.00% 39 8 0.00% 40 25 0.00% 41 28 0.00% 42 41 0.00% 43 52 0.00% 44 39 0.00% 45 59 0.00% 46 66 0.00% 47 67 0.00% 48 99 0.00% 49 116 0.00% 50 148 0.00% 51 143 0.00% 52 183 0.00% 53 184 0.00% 54 235 0.00% 55 266 0.00% 56 265 0.00% 57 376 0.00% 58 391 0.00% 59 485 0.00% 60 616 0.00% 61 737 0.00% 62 745 0.00% 63 879 0.00% 64 1009 0.00% 65 1064 0.01% 66 1264 0.01% 67 1435 0.01% 68 1754 0.01% 69 1879 0.01% 70 2216 0.01% 71 2630 0.01% 72 2816 0.01% 73 3285 0.02% 74 3872 0.02% 75 4073 0.02% 76 4533 0.02% 77 5026 0.02% 78 5486 0.03% 79 5971 0.03% 80 6505 0.03% 81 7342 0.04% 82 7876 0.04% 83 9035 0.04% 84 10149 0.05% 85 10848 0.05% 86 11606 0.06% 87 12389 0.06% 88 12816 0.06% 89 13565 0.06% 90 14734 0.07% 91 15369 0.07% 92 16319 0.08% 93 17366 0.08% 94 19100 0.09% 95 19983 0.10% 96 20899 0.10% 97 22377 0.11% 98 22963 0.11% 99 23449 0.11% 100 24543 0.12% 101 25095 0.12% 102 26136 0.13% 103 27194 0.13% 104 29142 0.14% 105 30654 0.15% 106 32255 0.15% 107 32856 0.16% 108 34106 0.16% 109 35577 0.17% 110 35984 0.17% 111 36197 0.17% 112 37393 0.18% 113 38760 0.19% 114 40771 0.20% 115 42013 0.20% 116 44506 0.21% 117 44842 0.21% 118 46153 0.22% 119 46455 0.22% 120 48121 0.23% 121 48623 0.23% 122 49336 0.24% 123 50880 0.24% 124 52398 0.25% 125 54213 0.26% 126 55998 0.27% 127 56426 0.27% 128 57627 0.28% 129 59005 0.28% 130 60011 0.29% 131 60541 0.29% 132 61491 0.29% 133 61984 0.30% 134 63340 0.30% 135 64314 0.31% 136 65979 0.32% 137 68045 0.33% 138 69002 0.33% 139 70785 0.34% 140 71300 0.34% 141 71542 0.34% 142 71936 0.34% 143 73119 0.35% 144 73517 0.35% 145 75645 0.36% 146 76157 0.36% 147 77379 0.37% 148 79084 0.38% 149 79699 0.38% 150 80283 0.38% 151 17816836 85.31% 20884608 reads passed initial QC criterion=sequence-density sequence-density=0.81 sequence-density-rank=1 fanout-score=2.43 fanout-score-rank=7 prefix-density=0.88 prefix-fanout=2.2 sequence=CTGATGCACTGCACTTGACG criterion=fanout-score sequence-density=0.01 sequence-density-rank=25 fanout-score=16.29 fanout-score-rank=1 prefix-density=0.03 prefix-fanout=3.9 sequence=AGCAGAATCAAGCTTCCGATTAAAGATACATAATTCCATGGAATGGAACCAACAAAGCAGCAGGAAATACAAGACACTTACAGATTACTAGCCATCAAATGAGATCCTGTAGAAAGGATTTGAGGAGGCCATGGCTAGCTAACTGTACTTTAATTTACAGCAAATACTATATTAGACAAACATGGAGTGACCAGACTTCTTCTTTTTATTTATTATAGTTCCATAAAACTGCTTGGTTGGAGCCATGCGGCGACGTTTTCTCATTTGCAGGAGCATGGATCACAGGTGCAGTTTGATCCACATTTGCAGCCATTCTCAGCACCAAAGTTCATCTCAGAGCTCTCGTAGAACATCCTAACTGGAGCTACACCAGCAATGATTGTCTGACTTGTGGTGGTCTCGGAGAAACTCAAGTCTGGGTACATGCTGCATCCATTGCAGCCACTGCCGCACTTGCA criterion=sequence-density sequence-density=0.65 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=24 prefix-density=0.65 prefix-fanout=2.0 sequence=CCAGGGTACTATGATGGACGCTACTGGACTATGTGGAA criterion=fanout-score sequence-density=0.30 sequence-density-rank=20 fanout-score=11.99 fanout-score-rank=1 prefix-density=0.67 prefix-fanout=5.4 sequence=AGCAATGGCAGCA SRR13695493 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 07:34:44 Started mapping on | Feb 12 07:34:44 Finished on | Feb 12 07:36:39 Mapping speed, Million of reads per hour | 653.78 Number of input reads | 20884608 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 19981857 Uniquely mapped reads % | 95.68% Average mapped length | 293.12 Number of splices: Total | 19697745 Number of splices: Annotated (sjdb) | 19362750 Number of splices: GT/AG | 19264438 Number of splices: GC/AG | 376485 Number of splices: AT/AC | 10571 Number of splices: Non-canonical | 46251 Mismatch rate per base, % | 0.35% Deletion rate per base | 0.02% Deletion average length | 2.75 Insertion rate per base | 0.02% Insertion average length | 2.31 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 458818 % of reads mapped to multiple loci | 2.20% Number of reads mapped to too many loci | 38905 % of reads mapped to too many loci | 0.19% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 1.85% % of reads unmapped: other | 0.09% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 444174 444174 444174 N_multimapping 458818 458818 458818 N_noFeature 445264 19568089 614072 N_ambiguous 377450 1349 131623 UnstrandedReadsAssigned:19159143 PositiveStrandReadsAssigned:412419 NegativeStrandReadsAssigned:19236162 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695493 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695493-trimmed-pair1.fastq SRR13695493-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,884,608 reads, 19,287,376 reads pseudoaligned [quant] estimated average fragment length: 231.74 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,173 rounds 52401 SRR13695493.ke.tsv 34699 SRR13695493.se.tsv 87100 total ==> SRR13695493.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1787.26 368 8.97559 Potri.005G024800.1.v4.1 1035 804.26 117 6.34151 Potri.004G059700.1.v4.1 961 730.3 1 0.05969 Potri.007G009000.2.v4.1 1416 1185.26 0 0 Potri.003G141000.2.v4.1 2943 2712.26 805 12.938 Potri.016G087400.1.v4.1 270 88.4738 999 492.214 Potri.015G069301.1.v4.1 564 337.973 0 0 Potri.010G195200.1.v4.1 1773 1542.26 30 0.847943 Potri.012G127500.1.v4.1 977 746.3 124 7.24288 ==> SRR13695493.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 157 Potri.001G233950.v4.1 1 Potri.001G122700.v4.1 454 Potri.001G212900.v4.1 11 Potri.001G182400.v4.1 4 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 25 SRR13695493 completed mapping pipeline successfully