Starting /dee2/code/volunteer_pipeline.sh SRR13695494 current disk space = 3050232999936 free memory = 1094826516 SRR13695494 SRAfilesize 5497a043da3f31a67173c776573c4531 SRR13695494.sra SRR13695494.sra file validated SRR13695494 is paired end SRR13695494 is conventional basespace SRR13695494 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695494_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5245 37.0 37.0 37.0 37.0 37.0 2 36.3095 37.0 37.0 37.0 37.0 37.0 3 36.506 37.0 37.0 37.0 37.0 37.0 4 36.5425 37.0 37.0 37.0 37.0 37.0 5 36.598 37.0 37.0 37.0 37.0 37.0 6 36.502 37.0 37.0 37.0 37.0 37.0 7 36.528 37.0 37.0 37.0 37.0 37.0 8 36.6115 37.0 37.0 37.0 37.0 37.0 9 36.511 37.0 37.0 37.0 37.0 37.0 10-14 36.5328 37.0 37.0 37.0 37.0 37.0 15-19 36.4953 37.0 37.0 37.0 37.0 37.0 20-24 36.4939 37.0 37.0 37.0 37.0 37.0 25-29 36.4229 37.0 37.0 37.0 37.0 37.0 30-34 36.444100000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.391099999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.4123 37.0 37.0 37.0 37.0 37.0 45-49 36.4224 37.0 37.0 37.0 37.0 37.0 50-54 36.36110000000001 37.0 37.0 37.0 37.0 37.0 55-59 36.2803 37.0 37.0 37.0 37.0 37.0 60-64 36.3523 37.0 37.0 37.0 37.0 37.0 65-69 36.306400000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.3183 37.0 37.0 37.0 37.0 37.0 75-79 36.289699999999996 37.0 37.0 37.0 37.0 37.0 80-84 36.192600000000006 37.0 37.0 37.0 37.0 37.0 85-89 36.2208 37.0 37.0 37.0 37.0 37.0 90-94 36.1827 37.0 37.0 37.0 37.0 37.0 95-99 36.104 37.0 37.0 37.0 37.0 37.0 100-104 36.096900000000005 37.0 37.0 37.0 37.0 37.0 105-109 36.0822 37.0 37.0 37.0 37.0 37.0 110-114 36.1124 37.0 37.0 37.0 37.0 37.0 115-119 36.1345 37.0 37.0 37.0 37.0 37.0 120-124 36.0079 37.0 37.0 37.0 37.0 37.0 125-129 35.959500000000006 37.0 37.0 37.0 37.0 37.0 130-134 35.9979 37.0 37.0 37.0 37.0 37.0 135-139 35.9098 37.0 37.0 37.0 37.0 37.0 140-144 35.825199999999995 37.0 37.0 37.0 37.0 37.0 145-149 35.68 37.0 37.0 37.0 37.0 37.0 150-151 35.52975 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 2.0 26 8.0 27 18.0 28 14.0 29 25.0 30 29.0 31 44.0 32 50.0 33 67.0 34 98.0 35 309.0 36 2935.0 37 401.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.8 12.7 9.325 40.175 2 19.899749373433583 12.781954887218044 36.51629072681704 30.802005012531332 3 17.675 16.375 27.6 38.35 4 23.45 22.55 23.799999999999997 30.2 5 23.375 30.225 24.3 22.1 6 22.35 31.2 24.2 22.25 7 15.65 28.599999999999998 37.875 17.875 8 17.125 27.0 32.625 23.25 9 17.299999999999997 23.65 33.925 25.124999999999996 10-14 18.9 29.395 28.349999999999998 23.355 15-19 19.005 28.27 27.800000000000004 24.925 20-24 19.93 28.804999999999996 27.534999999999997 23.73 25-29 20.735 28.425 27.295 23.544999999999998 30-34 19.355 30.035 26.974999999999998 23.635 35-39 19.74 29.25 27.150000000000002 23.86 40-44 19.830000000000002 28.915000000000003 27.525 23.73 45-49 19.905 28.985 27.435 23.674999999999997 50-54 20.285 28.125 27.735 23.855 55-59 20.16 28.02 27.33 24.490000000000002 60-64 20.04 29.110000000000003 27.565 23.285 65-69 20.665 28.42 26.88 24.035 70-74 20.34 28.375 27.284999999999997 24.0 75-79 19.950000000000003 28.494999999999997 27.560000000000002 23.995 80-84 21.035 27.99 27.584999999999997 23.39 85-89 20.085 28.865000000000002 27.33 23.72 90-94 20.735 27.725 27.755000000000003 23.785 95-99 20.465 27.565 27.82 24.15 100-104 20.5 27.99 27.71 23.799999999999997 105-109 20.65 27.99 26.935 24.425 110-114 20.369999999999997 28.105000000000004 27.3 24.224999999999998 115-119 21.12 28.645 26.405 23.830000000000002 120-124 20.685000000000002 28.575 27.189999999999998 23.549999999999997 125-129 20.865000000000002 28.175 26.505000000000003 24.455 130-134 21.08 28.575 26.05 24.295 135-139 21.27 27.6 27.235 23.895 140-144 21.05 28.515 26.384999999999998 24.05 145-149 21.37 27.785 26.52 24.325 150-151 21.512500000000003 28.9875 24.712500000000002 24.7875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 2.5 24 4.5 25 3.5 26 6.0 27 9.5 28 10.5 29 13.5 30 14.0 31 16.5 32 31.0 33 41.0 34 48.5 35 67.5 36 79.5 37 102.5 38 129.0 39 155.5 40 187.5 41 209.0 42 239.0 43 260.0 44 266.5 45 259.0 46 240.0 47 240.5 48 228.5 49 208.5 50 180.5 51 161.5 52 142.5 53 105.0 54 94.5 55 75.0 56 48.0 57 36.0 58 29.5 59 24.5 60 15.5 61 4.5 62 1.5 63 1.0 64 0.5 65 1.5 66 1.5 67 0.5 68 0.0 69 0.0 70 0.0 71 1.0 72 1.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.25 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.175 #Duplication Level Percentage of deduplicated Percentage of total 1 70.8348391760029 49.0 2 19.082038308637514 26.400000000000002 3 6.4691001084206725 13.425 4 2.493675460787857 6.9 5 0.8312251535959523 2.875 6 0.07228044813877846 0.3 7 0.14456089627755692 0.7000000000000001 8 0.07228044813877846 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCTAACCCTAACCCTGGATACACATACCTGAAGCATAATTAAGAAACAA 8 0.2 No Hit GTGCAGCTCTTTGGGGTCACAGAAAAGTTTAGCAGCATCTTGAACATGTA 8 0.2 No Hit ACTCTCTGTCCTTCCTCTTCCTCCCACTCACAATCCCACTCTTCTGCCTC 7 0.17500000000000002 No Hit CTCGAATAAATTCAAGGATATATTCGAAGAAATTCTGACCGTCAGTAGGA 7 0.17500000000000002 No Hit GCTCTATTGATTTCAAAAGGGGTTTTATGAAAAATTCAAATCCTTCCACA 7 0.17500000000000002 No Hit CACCTTCACTTTAACCAAATTCGGGCACCCATTTGCAAGCGCTTCCAATC 7 0.17500000000000002 No Hit CCATTCTTGAGTTCCTTCACCTTCAACTCAGCGAATGCCTCTGGGTCATC 6 0.15 No Hit GGATTCTTGATATTTCCCTGCAGCCATTTCGAGTCTAGCAAAGATAAGAC 6 0.15 No Hit CCTTAGCCAACCCAACTACCCCTTTAACATAATCCCCGTTGCCGGCTAAA 5 0.125 No Hit GCTCTTTTAACGACTTGACAGGAAAACAAAGTGGATGATTTCTTTGCTAA 5 0.125 No Hit CATCTCTCTTGCTGTCCTTTTCAAGTTCAGGAGATTCCTGATTCCAACCA 5 0.125 No Hit GGCCACCTACGTAGACATTCATGAATAGGACGCGTCAATAGACATGACGT 5 0.125 No Hit CCTTAGGACAAAATGTGCAATTTCTGGAACACATCGACTAGGCGGGATTC 5 0.125 No Hit GTTAGAGTTTCAATCCTTCATCAAGAGATTTGGTTGATCCAGAACGAATC 5 0.125 No Hit CATCAATTCCGTCCCCTATCACCATCACCGTCAATTTATTTGCCTTCCTG 5 0.125 No Hit GTAATGTAATAACACATAATCCTGAAGTTCAATCCCAGCTATAAAACAAG 5 0.125 No Hit ATCCATCAAAACATGCCCTCAATTGCTTGCTAAATCCTCAAGGTAACTGC 5 0.125 No Hit CATCCAAACATGGGTAGTTTCCACATAGTCCAGTAGCGTCCATCATAGTA 5 0.125 No Hit GGCTAAGTCTGCAATAATCCAGCTTGCGCCTTCTACACTAAATTATTTTC 5 0.125 No Hit GCCGTTTTGGGCCTTGCTAGAAGTGATAGGGGAAGTGACAGTACTTGTAG 5 0.125 No Hit GTTCCTGATTGTGCTTGTTGGATTACATCAAGTCCCTTGCAGAATGGGAC 5 0.125 No Hit TCAAAGTTTCTCACTCCACTGTCATTATTTGAAGCCATGAAATGAACTGC 5 0.125 No Hit CTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCG 5 0.125 No Hit GCCCGTTCCAGAAAGAAACAGCTACATGGCAAACTATAAATCCAAAATTC 5 0.125 No Hit AGAGAAACAGGATTTGATATATCTGTAGCTAGTGAGATAATGGCAGTTTT 5 0.125 No Hit GGCCACTTGCATCCTGCATCCGACATTCCCTCTTGAAGCTTGTATGGGTT 5 0.125 No Hit TGGCGGAGAACCAGTATGGGAGCATTGCCCCAACAATCAGCCCAATGAAG 5 0.125 No Hit GTGGTAAGCAGCCTCTAGAGCTCCAGTGGTGCTGAAAGTCCAGCAAGATC 5 0.125 No Hit TGCACCTTATCCTCAAAACCAAATTCCAAATTATTGATGGCAACAACAGA 5 0.125 No Hit GTGGGCGGGAACATGCATGCATATAATTAAGCTCTTTCGAACTCTTTATT 5 0.125 No Hit ATCGGGAGAAGCTTATGAAGATGGCCAGTGCAGTTCGCACTGGTGGAAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.0875 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.1875 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.325 0.0 0.0 0.0 0.0 88-89 0.3875 0.0 0.0 0.0 0.0 90-91 0.4625 0.0 0.0 0.0 0.0 92-93 0.7125 0.0 0.0 0.0 0.0 94-95 0.875 0.0 0.0 0.0 0.0 96-97 0.95 0.0 0.0 0.0 0.0 98-99 1.125 0.0 0.0 0.0 0.0 100-101 1.3 0.0 0.0 0.0 0.0 102-103 1.5125 0.0 0.0 0.0 0.0 104-105 1.675 0.0 0.0 0.0 0.0 106-107 1.8624999999999998 0.0 0.0 0.0 0.0 108-109 2.1375 0.0 0.0 0.0 0.0 110-111 2.4 0.0 0.0 0.0 0.0 112-113 2.55 0.0 0.0 0.0 0.0 114-115 2.8125 0.0 0.0 0.0 0.0 116-117 3.4 0.0 0.0 0.0 0.0 118-119 4.050000000000001 0.0 0.0 0.0 0.0 120-121 4.875 0.0 0.0 0.0 0.0 122-123 5.2 0.0 0.0 0.0 0.0 124-125 5.55 0.0 0.0 0.0 0.0 126-127 5.8625 0.0 0.0 0.0 0.0 128-129 6.3375 0.0 0.0 0.0 0.0 130-131 6.7875 0.0 0.0 0.0 0.0 132-133 7.15 0.0 0.0 0.0 0.0 134-135 7.699999999999999 0.0 0.0 0.0 0.0 136-137 8.2375 0.0 0.0 0.0 0.0 138-139 9.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCAAG 10 0.006830828 145.0 1 CTCTTCA 10 0.006830828 145.0 145 CTCTACA 10 0.006830828 145.0 8 AACCATG 10 0.006830828 145.0 9 TCTCTAC 10 0.006830828 145.0 7 >>END_MODULE SRR13695494 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695494_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.23875 37.0 37.0 37.0 37.0 37.0 2 36.1235 37.0 37.0 37.0 37.0 37.0 3 36.152 37.0 37.0 37.0 37.0 37.0 4 36.338 37.0 37.0 37.0 37.0 37.0 5 36.287 37.0 37.0 37.0 37.0 37.0 6 36.26 37.0 37.0 37.0 37.0 37.0 7 36.2405 37.0 37.0 37.0 37.0 37.0 8 36.31 37.0 37.0 37.0 37.0 37.0 9 36.334 37.0 37.0 37.0 37.0 37.0 10-14 36.314499999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.3062 37.0 37.0 37.0 37.0 37.0 20-24 36.21545 37.0 37.0 37.0 37.0 37.0 25-29 36.22555 37.0 37.0 37.0 37.0 37.0 30-34 36.20695 37.0 37.0 37.0 37.0 37.0 35-39 36.18065 37.0 37.0 37.0 37.0 37.0 40-44 36.15105 37.0 37.0 37.0 37.0 37.0 45-49 36.15689999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.121950000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.107749999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.059549999999994 37.0 37.0 37.0 37.0 37.0 65-69 36.0455 37.0 37.0 37.0 37.0 37.0 70-74 36.02685 37.0 37.0 37.0 37.0 37.0 75-79 36.03995 37.0 37.0 37.0 37.0 37.0 80-84 36.0185 37.0 37.0 37.0 37.0 37.0 85-89 35.949349999999995 37.0 37.0 37.0 37.0 37.0 90-94 35.92155 37.0 37.0 37.0 37.0 37.0 95-99 35.934749999999994 37.0 37.0 37.0 37.0 37.0 100-104 35.87415 37.0 37.0 37.0 37.0 37.0 105-109 35.921549999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.73795 37.0 37.0 37.0 37.0 37.0 115-119 35.78595 37.0 37.0 37.0 37.0 37.0 120-124 35.679649999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.73180000000001 37.0 37.0 37.0 37.0 37.0 130-134 35.59785000000001 37.0 37.0 37.0 37.0 37.0 135-139 35.65455 37.0 37.0 37.0 37.0 37.0 140-144 35.54625 37.0 37.0 37.0 37.0 37.0 145-149 35.390049999999995 37.0 37.0 37.0 37.0 37.0 150-151 35.132000000000005 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 5.0 15 0.0 16 2.0 17 1.0 18 1.0 19 1.0 20 2.0 21 0.0 22 3.0 23 2.0 24 5.0 25 6.0 26 11.0 27 7.0 28 11.0 29 17.0 30 20.0 31 34.0 32 50.0 33 109.0 34 186.0 35 534.0 36 2754.0 37 239.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.44052091159529 22.28900576008014 12.972702228900577 27.29777109942399 2 27.3 26.525 28.675 17.5 3 21.675 28.425 30.95 18.95 4 23.775 33.5 24.05 18.675 5 28.4 33.95 21.75 15.9 6 20.8 41.425 20.549999999999997 17.224999999999998 7 21.8 23.1 35.925000000000004 19.175 8 21.825 28.575 25.974999999999998 23.625 9 22.175 25.624999999999996 29.599999999999998 22.6 10-14 23.1 29.99 25.82 21.09 15-19 24.305 28.465 26.495 20.735 20-24 23.276163808190407 29.091454572728637 27.256362818140907 20.376018800940045 25-29 23.615903975993998 27.826956739184794 27.666916729182294 20.89022255563891 30-34 23.65354803220483 28.609291393709057 26.699004850727608 21.038155723358503 35-39 23.241162058102905 28.366418320916047 26.736336816840844 21.656082804140205 40-44 22.20833124968745 28.694304145621842 27.78916837525629 21.308196229434415 45-49 23.097309730973098 28.07780778077808 27.847784778477845 20.977097709770977 50-54 24.46122306115306 28.311415570778536 25.881294064703237 21.346067303365167 55-59 23.905976494123532 27.27181795448862 28.017004251062765 20.80520130032508 60-64 23.076153807690382 27.85139256962848 26.986349317465873 22.086104305215258 65-69 23.405 27.855 27.395000000000003 21.345 70-74 24.626156539134783 27.936984246061513 27.49687421855464 19.939984996249063 75-79 24.458668800320048 27.254088113216984 27.124068610291545 21.163174476171427 80-84 23.395 28.92 26.415 21.27 85-89 24.241060265066267 28.292073018254566 26.65666416604151 20.810202550637662 90-94 24.206210310515523 28.401420071003553 26.58632931646582 20.8060403020151 95-99 23.661183059152957 28.596429821491075 26.986349317465873 20.756037801890095 100-104 24.191047761940485 27.156789197299325 28.442110527631908 20.210052513128282 105-109 23.6609152288072 27.261815453863463 27.741935483870968 21.335333833458364 110-114 24.64123206160308 27.896394819740987 27.05635281764088 20.40602030101505 115-119 24.976244061015255 28.442110527631908 26.301575393848463 20.280070017504375 120-124 24.216054013503378 27.691922980745186 27.671917979494875 20.420105026256564 125-129 24.99499899979996 27.770554110822165 26.960392078415683 20.27405481096219 130-134 25.646411602900727 26.911727931983 27.371842960740185 20.070017504376096 135-139 25.661415353838457 26.98674668667167 27.406851712928233 19.94498624656164 140-144 25.121256062803138 28.286414320716034 26.291314565728285 20.30101505075254 145-149 26.47161790447612 27.67691922980745 26.66166541635409 19.18979744936234 150-151 26.244061015253813 26.531632908227053 27.481870467616904 19.742435608902227 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.5 9 0.5 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 1.5 20 0.5 21 1.0 22 1.5 23 1.5 24 1.0 25 1.5 26 5.5 27 6.0 28 5.5 29 8.0 30 11.0 31 15.5 32 18.0 33 26.5 34 31.0 35 44.5 36 81.5 37 113.5 38 132.5 39 153.5 40 184.0 41 215.5 42 257.0 43 271.5 44 264.5 45 277.0 46 275.0 47 279.0 48 235.0 49 185.0 50 167.0 51 149.0 52 130.0 53 99.0 54 87.5 55 57.0 56 47.5 57 44.0 58 26.5 59 21.5 60 17.5 61 16.5 62 10.0 63 3.5 64 1.0 65 0.0 66 0.5 67 0.5 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17500000000000002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.015 35-39 0.005 40-44 0.015 45-49 0.01 50-54 0.005 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.015 80-84 0.0 85-89 0.025 90-94 0.005 95-99 0.005 100-104 0.025 105-109 0.025 110-114 0.005 115-119 0.025 120-124 0.025 125-129 0.02 130-134 0.025 135-139 0.025 140-144 0.005 145-149 0.025 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.675 #Duplication Level Percentage of deduplicated Percentage of total 1 71.36706135629709 49.725 2 19.052744886975244 26.55 3 6.027987082884822 12.6 4 2.5116612845353425 7.000000000000001 5 0.6817366343738788 2.375 6 0.14352350197344815 0.6 7 0.14352350197344815 0.7000000000000001 8 0.03588087549336204 0.2 9 0.0 0.0 >10 0.03588087549336204 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 10 0.25 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 8 0.2 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 7 0.17500000000000002 No Hit GGTTGAATCAAAATAATTTCTTGTAAAGTTTTCTTTCTTTCAGAGGACAA 7 0.17500000000000002 No Hit GATGACCTGGATATTCCTTAACCAAATTCAAAAGCTGGAGAAATCAGTCG 7 0.17500000000000002 No Hit ATCCTCACGACTTGCTAGGTATAACAAGAAGCCCACAATTACCTCGAGGG 7 0.17500000000000002 No Hit TCCTCAAACAACCAATTACTCTCTTTGGTGAAGATGACGATGCTCGTCTT 6 0.15 No Hit CGGGGTGAATCCTACACAGATTAGTTTAGAATTGTTAGCTTCAAATTGTC 6 0.15 No Hit ACTGAGAATCACTTAGATTAATTCACTGTTAGAAACTAATTATTGATTAT 6 0.15 No Hit ATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAAT 6 0.15 No Hit CCAAGCTTGATCCACGCACAAAGCATCTTGGCCATCTGGGCTACACAGGT 5 0.125 No Hit GCTTGAACCTGATCATGCCCCCCAGGGGTGGGGATGGCAATCTCTCTCAG 5 0.125 No Hit CCTATAGATGGGAATATTTTGGGTACCGGGAAGGTTTCCACAGTTAACAC 5 0.125 No Hit TGAGAAAAAGAAAAGAGTAGGAACATTTTATTTATTGGTAGTCCAAGGTC 5 0.125 No Hit CCCACGTGTTGGCAGCGATGGATGCTTGCACTTTGGGATTTAGAAACTGG 5 0.125 No Hit CAACTGTCAACGCTACTGTTGATATTTACCTTCGTTCTAATGATTACTAA 5 0.125 No Hit GGCGGTCCAAACTATCAGACTGGTTTGGCCAAGATGCGCACTCTGGGAGA 5 0.125 No Hit GTTTGATGCTCGTCAATTTGATTCTAGGATGAATGAGCTGCTCACAGCTG 5 0.125 No Hit TATCTTCAAATGCAACTCGGGGTCCGGAAAGCTCAGCGACATTTTCTTTG 5 0.125 No Hit AATACAACTGGATGGAAAAGTGGAGCAAGTGGGATTGGCACCCAGAAGTC 5 0.125 No Hit TCATCAGATATGCTAGCCTGTGGGGAAGATCTGGGTCTAATTCCTGCTTG 5 0.125 No Hit CATCAGCTGTCACAGGGTCCCCGGCCTTGCTATTGCCAATCACCATTTTA 5 0.125 No Hit AGAGCCTTTCTTGATCAATTATGTACGAAAATTGTGGAGACTGATATGGG 5 0.125 No Hit GTGCTAGAACCATGTGAAGGATGTGGCCCACATCACAGCTACCAAGGTCA 5 0.125 No Hit GTTTCCTGTATGGAGATTTCACCTATGTTTGCAGACTCGGGCAATTACTT 5 0.125 No Hit CTCCAAGTTTAGAAGAAGTAAGCAAAGACATGGTGGATAGCTATTTCTCC 5 0.125 No Hit CTCTTAGAAGGATTCACACAAACTCAAGTTAGTACACTGGCAGTAAAGGA 5 0.125 No Hit TGTTTGGTTTCTAAGAAAATCCAGATACAAAAAGGGAAAAGGAAAAAAAA 5 0.125 No Hit GTTCGAGACTCTTTCTTACCTTCCAGATCTCACTACTGAGCAATTGGCCC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.0875 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.1875 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.325 0.0 0.0 0.0 0.0 88-89 0.3875 0.0 0.0 0.0 0.0 90-91 0.4625 0.0 0.0 0.0 0.0 92-93 0.7125 0.0 0.0 0.0 0.0 94-95 0.875 0.0 0.0 0.0 0.0 96-97 0.95 0.0 0.0 0.0 0.0 98-99 1.125 0.0 0.0 0.0 0.0 100-101 1.2875 0.0 0.0 0.0 0.0 102-103 1.4875 0.0 0.0 0.0 0.0 104-105 1.6625 0.0 0.0 0.0 0.0 106-107 1.8875000000000002 0.0 0.0 0.0 0.0 108-109 2.1625 0.0 0.0 0.0 0.0 110-111 2.425 0.0 0.0 0.0 0.0 112-113 2.5875 0.0 0.0 0.0 0.0 114-115 2.8625 0.0 0.0 0.0 0.0 116-117 3.45 0.0 0.0 0.0 0.0 118-119 4.1 0.0 0.0 0.0 0.0 120-121 4.9375 0.0 0.0 0.0 0.0 122-123 5.275 0.0 0.0 0.0 0.0 124-125 5.637499999999999 0.0 0.0 0.0 0.0 126-127 5.9625 0.0 0.0 0.0 0.0 128-129 6.4125 0.0 0.0 0.0 0.0 130-131 6.8625 0.0 0.0 0.0 0.0 132-133 7.225 0.0 0.0 0.0 0.0 134-135 7.824999999999999 0.0 0.0 0.0 0.0 136-137 8.3875 0.0 0.0 0.0 0.0 138-139 9.125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTCCA 10 0.006830828 145.0 4 >>END_MODULE Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809275 spots for SRR13695494.sra Written 809275 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra Read 809264 spots for SRR13695494.sra Written 809264 spots for SRR13695494.sra SRR ids: ['SRR13695494.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_vy27s6xh SRR13695494.sra spots: 16185291 blocks: [[1, 809264], [809265, 1618528], [1618529, 2427792], [2427793, 3237056], [3237057, 4046320], [4046321, 4855584], [4855585, 5664848], [5664849, 6474112], [6474113, 7283376], [7283377, 8092640], [8092641, 8901904], [8901905, 9711168], [9711169, 10520432], [10520433, 11329696], [11329697, 12138960], [12138961, 12948224], [12948225, 13757488], [13757489, 14566752], [14566753, 15376016], [15376017, 16185291]] SRR13695494 file size 5478769 SRR13695494 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695494 SRR13695494_1.fastq SRR13695494_2.fastq Input file: SRR13695494_1.fastq Paired file: SRR13695494_2.fastq trimmed: SRR13695494-trimmed-pair1.fastq, SRR13695494-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 07:00:55 2025 >> started Wed Feb 12 07:01:13 2025 >> done (18.357s) 16185291 read pairs processed; of these: 108 ( 0.00%) short read pairs filtered out after trimming by size control 848 ( 0.01%) empty read pairs filtered out after trimming by size control 16184335 (99.99%) read pairs available; of these: 2178660 (13.46%) trimmed read pairs available after processing 14005675 (86.54%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 2 0.00% 20 1 0.00% 21 2 0.00% 22 2 0.00% 23 0 0.00% 24 3 0.00% 25 1 0.00% 26 0 0.00% 27 5 0.00% 28 3 0.00% 29 2 0.00% 30 2 0.00% 31 2 0.00% 32 5 0.00% 33 1 0.00% 34 1 0.00% 35 2 0.00% 36 1 0.00% 37 2 0.00% 38 5 0.00% 39 2 0.00% 40 4 0.00% 41 14 0.00% 42 3 0.00% 43 13 0.00% 44 10 0.00% 45 13 0.00% 46 7 0.00% 47 10 0.00% 48 30 0.00% 49 35 0.00% 50 38 0.00% 51 42 0.00% 52 48 0.00% 53 49 0.00% 54 53 0.00% 55 67 0.00% 56 66 0.00% 57 75 0.00% 58 112 0.00% 59 116 0.00% 60 146 0.00% 61 183 0.00% 62 213 0.00% 63 213 0.00% 64 250 0.00% 65 340 0.00% 66 359 0.00% 67 419 0.00% 68 404 0.00% 69 530 0.00% 70 654 0.00% 71 818 0.01% 72 966 0.01% 73 1116 0.01% 74 1263 0.01% 75 1456 0.01% 76 1535 0.01% 77 1885 0.01% 78 2009 0.01% 79 2379 0.01% 80 2601 0.02% 81 2933 0.02% 82 3296 0.02% 83 3957 0.02% 84 4583 0.03% 85 4936 0.03% 86 5480 0.03% 87 5845 0.04% 88 6363 0.04% 89 7025 0.04% 90 7844 0.05% 91 8245 0.05% 92 8783 0.05% 93 9752 0.06% 94 10787 0.07% 95 11343 0.07% 96 12389 0.08% 97 13248 0.08% 98 13732 0.08% 99 14556 0.09% 100 15654 0.10% 101 15880 0.10% 102 16721 0.10% 103 17426 0.11% 104 19088 0.12% 105 19674 0.12% 106 21060 0.13% 107 21944 0.14% 108 22658 0.14% 109 24188 0.15% 110 24256 0.15% 111 25148 0.16% 112 26585 0.16% 113 27000 0.17% 114 27925 0.17% 115 28855 0.18% 116 30847 0.19% 117 31870 0.20% 118 33292 0.21% 119 33859 0.21% 120 34689 0.21% 121 35796 0.22% 122 35979 0.22% 123 37241 0.23% 124 38663 0.24% 125 39257 0.24% 126 40830 0.25% 127 41936 0.26% 128 42709 0.26% 129 43288 0.27% 130 45490 0.28% 131 45304 0.28% 132 46081 0.28% 133 46729 0.29% 134 47956 0.30% 135 48574 0.30% 136 49350 0.30% 137 50836 0.31% 138 51927 0.32% 139 53700 0.33% 140 54459 0.34% 141 54852 0.34% 142 55683 0.34% 143 56117 0.35% 144 57499 0.36% 145 58517 0.36% 146 58628 0.36% 147 59928 0.37% 148 61350 0.38% 149 62343 0.39% 150 63334 0.39% 151 14005675 86.54% 16184335 reads passed initial QC criterion=sequence-density sequence-density=0.54 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=32 prefix-density=0.53 prefix-fanout=2.0 sequence=GTGTTGTCGAATCC criterion=fanout-score sequence-density=0.03 sequence-density-rank=34 fanout-score=40.06 fanout-score-rank=1 prefix-density=0.16 prefix-fanout=7.0 sequence=AATGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.49 sequence-density-rank=1 fanout-score=2.26 fanout-score-rank=26 prefix-density=0.51 prefix-fanout=2.2 sequence=GCTCGAGAATTGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=25.33 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=2.1 sequence=CCACCACTAAACCCAAAGCTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAATGGCCCTCTCCTCCCCTTCGCTAGCCGGAAAGGCGGTGAAGCTCAACCCCTCCTCCTCTGAGATCATGGGCAATGGCCGTGTCTCCATGAGGAAAACCACCAAGCCTGTTCCCTCCGGGAGCCCATGGTACGGACCAGACCGTGTTAAATACTTGGGCCCGTTCTCTGGTGAGCCCCCATCCTACTTGACTGGTGAGTTCCCTGGTGACTACGGCTGGGACACTGCTGGCCTCTCTGCTGACCCAGAGACCTTTGCCAAGAACCGTGAGCTTGAAGTCATCCATTCCAGGTGGGCCATGCTTGGAGCTCTTGGATGCGTCTTCCCCGAGCTCTTGTCCCGCAACGGTGTCAAGTTCGGCGAGGCTGTATGGTTCAAGGCTGGAGCCCAGATCTTCAGCGAGGGTGGACTTGACTACTTGGGCAACCCAAGCTTGATCCAC SRR13695494 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 07:01:58 Started mapping on | Feb 12 07:01:59 Finished on | Feb 12 07:03:49 Mapping speed, Million of reads per hour | 529.67 Number of input reads | 16184335 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 15306616 Uniquely mapped reads % | 94.58% Average mapped length | 294.30 Number of splices: Total | 15219271 Number of splices: Annotated (sjdb) | 14902083 Number of splices: GT/AG | 14904785 Number of splices: GC/AG | 253760 Number of splices: AT/AC | 9409 Number of splices: Non-canonical | 51317 Mismatch rate per base, % | 0.36% Deletion rate per base | 0.03% Deletion average length | 2.88 Insertion rate per base | 0.02% Insertion average length | 2.32 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 378653 % of reads mapped to multiple loci | 2.34% Number of reads mapped to too many loci | 38356 % of reads mapped to too many loci | 0.24% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.73% % of reads unmapped: other | 0.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 499270 499270 499270 N_multimapping 378653 378653 378653 N_noFeature 413731 14970173 569678 N_ambiguous 280261 1467 98963 UnstrandedReadsAssigned:14612624 PositiveStrandReadsAssigned:334976 NegativeStrandReadsAssigned:14637975 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695494 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695494-trimmed-pair1.fastq SRR13695494-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,184,335 reads, 14,689,402 reads pseudoaligned [quant] estimated average fragment length: 234.995 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,066 rounds 52401 SRR13695494.ke.tsv 34699 SRR13695494.se.tsv 87100 total ==> SRR13695494.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1784.01 587 18.1685 Potri.005G024800.1.v4.1 1035 801.005 382.086 26.3392 Potri.004G059700.1.v4.1 961 727.02 0 0 Potri.007G009000.2.v4.1 1416 1182.01 0 0 Potri.003G141000.2.v4.1 2943 2709.01 855 17.4274 Potri.016G087400.1.v4.1 270 86.5243 805 513.729 Potri.015G069301.1.v4.1 564 335.551 0 0 Potri.010G195200.1.v4.1 1773 1539.01 120 4.30544 Potri.012G127500.1.v4.1 977 743.015 68 5.05345 ==> SRR13695494.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 67 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 313 Potri.001G212900.v4.1 20 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 3 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695494 completed mapping pipeline successfully