Starting /dee2/code/volunteer_pipeline.sh SRR13695495 current disk space = 3049714888704 free memory = 1580772556 SRR13695495 SRAfilesize ad8947001b338357b79d0ddf8aa1d7ff SRR13695495.sra SRR13695495.sra file validated SRR13695495 is paired end SRR13695495 is conventional basespace SRR13695495 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695495_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.531 37.0 37.0 37.0 37.0 37.0 2 36.20625 37.0 37.0 37.0 37.0 37.0 3 36.453 37.0 37.0 37.0 37.0 37.0 4 36.517 37.0 37.0 37.0 37.0 37.0 5 36.5935 37.0 37.0 37.0 37.0 37.0 6 36.5655 37.0 37.0 37.0 37.0 37.0 7 36.4735 37.0 37.0 37.0 37.0 37.0 8 36.6265 37.0 37.0 37.0 37.0 37.0 9 36.5445 37.0 37.0 37.0 37.0 37.0 10-14 36.5164 37.0 37.0 37.0 37.0 37.0 15-19 36.4833 37.0 37.0 37.0 37.0 37.0 20-24 36.530499999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.4399 37.0 37.0 37.0 37.0 37.0 30-34 36.398 37.0 37.0 37.0 37.0 37.0 35-39 36.357 37.0 37.0 37.0 37.0 37.0 40-44 36.401799999999994 37.0 37.0 37.0 37.0 37.0 45-49 36.329 37.0 37.0 37.0 37.0 37.0 50-54 36.361599999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.3056 37.0 37.0 37.0 37.0 37.0 60-64 36.2746 37.0 37.0 37.0 37.0 37.0 65-69 36.2879 37.0 37.0 37.0 37.0 37.0 70-74 36.2996 37.0 37.0 37.0 37.0 37.0 75-79 36.2577 37.0 37.0 37.0 37.0 37.0 80-84 36.195299999999996 37.0 37.0 37.0 37.0 37.0 85-89 36.1635 37.0 37.0 37.0 37.0 37.0 90-94 36.1111 37.0 37.0 37.0 37.0 37.0 95-99 36.0752 37.0 37.0 37.0 37.0 37.0 100-104 36.0744 37.0 37.0 37.0 37.0 37.0 105-109 36.0603 37.0 37.0 37.0 37.0 37.0 110-114 36.0226 37.0 37.0 37.0 37.0 37.0 115-119 36.056700000000006 37.0 37.0 37.0 37.0 37.0 120-124 35.9322 37.0 37.0 37.0 37.0 37.0 125-129 35.9129 37.0 37.0 37.0 37.0 37.0 130-134 35.94760000000001 37.0 37.0 37.0 37.0 37.0 135-139 35.8054 37.0 37.0 37.0 37.0 37.0 140-144 35.7745 37.0 37.0 37.0 37.0 37.0 145-149 35.4748 37.0 37.0 37.0 37.0 37.0 150-151 35.43425 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 1.0 23 1.0 24 1.0 25 2.0 26 8.0 27 9.0 28 24.0 29 24.0 30 29.0 31 23.0 32 42.0 33 79.0 34 140.0 35 350.0 36 2941.0 37 325.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.225 10.775 8.475000000000001 40.525 2 19.567947751821148 13.740266264757597 36.12157749309219 30.570208490329065 3 18.425 18.6 27.6 35.375 4 20.724999999999998 24.75 27.3 27.224999999999998 5 23.225 31.974999999999998 24.325 20.474999999999998 6 19.2 34.75 23.3 22.75 7 15.625 28.549999999999997 40.475 15.35 8 18.375 24.75 31.624999999999996 25.25 9 17.95 22.025 36.199999999999996 23.825 10-14 19.84 29.915000000000003 27.495000000000005 22.75 15-19 19.705000000000002 28.865000000000002 28.325 23.105 20-24 19.814999999999998 28.865000000000002 27.894999999999996 23.425 25-29 19.935 28.27 28.26 23.535 30-34 19.78 28.860000000000003 28.060000000000002 23.3 35-39 20.080000000000002 29.099999999999998 27.705000000000002 23.115 40-44 19.994999999999997 29.09 27.55 23.365 45-49 20.22 29.115000000000002 26.945000000000004 23.72 50-54 20.585 28.694999999999997 26.950000000000003 23.77 55-59 20.505000000000003 28.79 27.765 22.939999999999998 60-64 20.025000000000002 27.955000000000002 27.894999999999996 24.125 65-69 19.77 28.610000000000003 28.144999999999996 23.474999999999998 70-74 19.99 28.09 27.955000000000002 23.965 75-79 20.235 28.754999999999995 27.305 23.705000000000002 80-84 20.275000000000002 27.644999999999996 28.29 23.79 85-89 20.849999999999998 28.89 27.605 22.655 90-94 20.34 27.785 28.475 23.400000000000002 95-99 20.365 28.255000000000003 28.375 23.005 100-104 19.915 29.060000000000002 27.67 23.355 105-109 20.64 27.43 29.005 22.925 110-114 20.979999999999997 28.265 27.71 23.044999999999998 115-119 20.735 28.82 27.47 22.975 120-124 19.89 29.215000000000003 27.82 23.075000000000003 125-129 20.990000000000002 28.77 27.224999999999998 23.015 130-134 20.78 28.87 27.860000000000003 22.49 135-139 20.380000000000003 29.060000000000002 27.029999999999998 23.53 140-144 21.310000000000002 28.42 27.26 23.01 145-149 20.46 28.139999999999997 27.400000000000002 24.0 150-151 20.8125 27.800000000000004 27.437499999999996 23.95 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.5 15 1.5 16 0.0 17 0.5 18 0.5 19 0.5 20 1.0 21 2.5 22 4.0 23 2.0 24 3.0 25 6.0 26 6.0 27 8.0 28 9.5 29 11.0 30 15.5 31 21.0 32 35.0 33 48.0 34 48.5 35 71.0 36 95.5 37 124.0 38 158.0 39 166.0 40 191.5 41 226.0 42 235.0 43 240.5 44 255.5 45 275.0 46 269.0 47 250.0 48 226.5 49 193.5 50 160.5 51 132.5 52 110.0 53 88.5 54 65.5 55 52.0 56 48.5 57 34.0 58 29.0 59 27.0 60 20.0 61 14.5 62 6.5 63 0.5 64 2.0 65 2.0 66 1.0 67 1.0 68 1.0 69 0.5 70 0.0 71 0.0 72 0.0 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.475 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.25 #Duplication Level Percentage of deduplicated Percentage of total 1 71.35231316725978 50.125 2 19.8220640569395 27.85 3 5.5160142348754455 11.625 4 2.0640569395017794 5.800000000000001 5 0.9608540925266904 3.375 6 0.2491103202846975 1.05 7 0.03558718861209965 0.17500000000000002 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGAAAAGATTTAGAAGATCTAGTTCTCAGCAACAAAACAACCAAAACAA 7 0.17500000000000002 No Hit GTGGTTGACCTGCCATGCAAGCGCACCTGTCATGGCAAAGGTCGGATGAC 6 0.15 No Hit GTCTTTTCCAAAGCCAGTTGAAGATGATAGGTGGCATCAGAATGAAGTAA 6 0.15 No Hit GCAGGTCCGTTGAAGTTGGAGACGATATTAGAGGCCTTTACAAGAGGCAA 6 0.15 No Hit GTCATCTTCAAGAGGGCAGAGACAGTAACGCATATTACACACATATTTTA 6 0.15 No Hit TGCTAATCGAGTCTGGCTCTAGCTCAAACAAATTGTCTAGGTCAGGCGAA 6 0.15 No Hit CCTCCAAACACGAAAATGATAATTTTATTAAGATAGTGGTTGACCTGCCA 6 0.15 No Hit ACGGAAGTTCCTTTCTGACTAAAACTTATCTCCAATAGCTTGCTAGAAGA 6 0.15 No Hit CCTGGATCTTGACATCCTTTGGGGTCTTTGCTCCCAAATCAGTATCGGAT 5 0.125 No Hit CCTTTTGCTGCTTTTGATTCCTCTTATAGCTTCAGCATAATCTGGAGCTC 5 0.125 No Hit CTCACAACAGTACGCCATTTGGCGAAACGAGCACCCTGCTGGTAGTAAGC 5 0.125 No Hit CACCGCACTCAGCATTAGGGCACTCCTTCCTCAATCTCTGGACTTTGCCG 5 0.125 No Hit GGCTGTTATTCTCAAGGTAGCCATAGACCAAAATTCTATGGTTTCCATCT 5 0.125 No Hit ATCGGAGATACACTCCCATGCATGCTAAGCAACTCTCCTTGATGTGCCCA 5 0.125 No Hit CTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCT 5 0.125 No Hit AGAAGGAACTGTGCAAACAAGGAGGGCTTAATTTAGGGGTGTTTGAAAGA 5 0.125 No Hit CGTTTTCTTGCGGTGTACAACGCTGTTTCTGGCCTGTTTCTTCGCTCTCC 5 0.125 No Hit GAGGAGACAGCTGCGAGAAAGAAGAAAGAGAAAGGCGTGATGAGGATGAG 5 0.125 No Hit CTCCAAAGAAAGATCCTGTACTAGACTGCTTAAGGCCAACTGACATGATT 5 0.125 No Hit CGGTTTACTGTTCTTCTAGACATCACAATTTCCTTGGCAAGTGCCTTGGC 5 0.125 No Hit CTCGGTGATATTATTTGTTTGTTTTAAATATAAGTTGCATCGTCAAAAAG 5 0.125 No Hit GTTGACTTCTATCTTCATCTATGCCTTCGAAGTCTATCAATGGAACAGCC 5 0.125 No Hit GCAAGAGAGAGAGCAGAGAAAGCAGTGACAGCAACAACGACCAGAACCGA 5 0.125 No Hit CTAGTTTTAAAATTTTTAACCGTGTTCGAATACAAATGCATCAACATGGT 5 0.125 No Hit GCGTTGAAACCACAGTTTTGGTTGCATTATCTTGATAGCCCACTGTATCA 5 0.125 No Hit GCTTGTAGTAACCAAAAAAGAGGACATATTTGTACAAGTAATCACATTAA 5 0.125 No Hit GCTTCTTTTTTTGCCTGTCTAGCTTTAGTTCTGCCACTTCCTTGGCCTTC 5 0.125 No Hit GGCTCATCAAAAAGCTCCCATGAAACAAAGGAGACAACTCTCACAGCCTT 5 0.125 No Hit CTCTGATTTGACAATTTTGTCCTTCATATTCGCGATTTTCTTCAACTCAT 5 0.125 No Hit CAGTAAAAGACCCAGCTTTCTCTTCAAAAACCAAACCATTACCTAAAAAC 5 0.125 No Hit CAACAGCTGACACAGTTGATACTGCAAACTACCAGAACTACAAAATATAT 5 0.125 No Hit CTGGACTCCAGGGGCTCTAAGGAAATCAGCACAAGTGAGATGAGATATAT 5 0.125 No Hit CCTTCAAAAAGTTTCTCTTCTGAAGGAGGTCACTTCGCCTCATTCTCAAG 5 0.125 No Hit CACCACCCTTTCTCTTTCCCATATGCCCTTAGATCCTGAAGAGTATAAAC 5 0.125 No Hit GACCATTGTAGCAGTACTTCATGGATTGTTCTTCTGGGTATAGATGTAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.1 0.0 0.0 0.0 0.0 84-85 0.125 0.0 0.0 0.0 0.0 86-87 0.125 0.0 0.0 0.0 0.0 88-89 0.125 0.0 0.0 0.0 0.0 90-91 0.1875 0.0 0.0 0.0 0.0 92-93 0.2375 0.0 0.0 0.0 0.0 94-95 0.25 0.0 0.0 0.0 0.0 96-97 0.35 0.0 0.0 0.0 0.0 98-99 0.5375 0.0 0.0 0.0 0.0 100-101 0.6 0.0 0.0 0.0 0.0 102-103 0.7875 0.0 0.0 0.0 0.0 104-105 0.9125000000000001 0.0 0.0 0.0 0.0 106-107 0.9375 0.0 0.0 0.0 0.0 108-109 1.0499999999999998 0.0 0.0 0.0 0.0 110-111 1.2625000000000002 0.0 0.0 0.0 0.0 112-113 1.4875 0.0 0.0 0.0 0.0 114-115 1.6 0.0 0.0 0.0 0.0 116-117 1.8125 0.0 0.0 0.0 0.0 118-119 2.0625 0.0 0.0 0.0 0.0 120-121 2.375 0.0 0.0 0.0 0.0 122-123 2.8125 0.0 0.0 0.0 0.0 124-125 3.0625 0.0 0.0 0.0 0.0 126-127 3.4124999999999996 0.0 0.0 0.0 0.0 128-129 3.7125 0.0 0.0 0.0 0.0 130-131 4.0625 0.0 0.0 0.0 0.0 132-133 4.6875 0.0 0.0 0.0 0.0 134-135 4.8625 0.0 0.0 0.0 0.0 136-137 5.375 0.0 0.0 0.0 0.0 138-139 6.0875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13695495 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695495_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.01825 37.0 37.0 37.0 37.0 37.0 2 35.9235 37.0 37.0 37.0 37.0 37.0 3 36.0705 37.0 37.0 37.0 37.0 37.0 4 36.094 37.0 37.0 37.0 37.0 37.0 5 36.0555 37.0 37.0 37.0 37.0 37.0 6 36.1205 37.0 37.0 37.0 37.0 37.0 7 36.057 37.0 37.0 37.0 37.0 37.0 8 36.1695 37.0 37.0 37.0 37.0 37.0 9 36.1575 37.0 37.0 37.0 37.0 37.0 10-14 36.1321 37.0 37.0 37.0 37.0 37.0 15-19 36.1127 37.0 37.0 37.0 37.0 37.0 20-24 36.06385 37.0 37.0 37.0 37.0 37.0 25-29 35.99585 37.0 37.0 37.0 37.0 37.0 30-34 35.94995 37.0 37.0 37.0 37.0 37.0 35-39 35.98915 37.0 37.0 37.0 37.0 37.0 40-44 35.915049999999994 37.0 37.0 37.0 37.0 37.0 45-49 35.87155 37.0 37.0 37.0 37.0 37.0 50-54 35.83875 37.0 37.0 37.0 37.0 37.0 55-59 35.79375 37.0 37.0 37.0 37.0 37.0 60-64 35.735549999999996 37.0 37.0 37.0 37.0 37.0 65-69 35.70885 37.0 37.0 37.0 37.0 37.0 70-74 35.66245 37.0 37.0 37.0 37.0 37.0 75-79 35.648250000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.695350000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.619749999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.573750000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.53025 37.0 37.0 37.0 37.0 37.0 100-104 35.56905 37.0 37.0 37.0 37.0 37.0 105-109 35.54645000000001 37.0 37.0 37.0 37.0 37.0 110-114 35.46495 37.0 37.0 37.0 37.0 37.0 115-119 35.41425 37.0 37.0 37.0 34.6 37.0 120-124 35.45775 37.0 37.0 37.0 37.0 37.0 125-129 35.41125 37.0 37.0 37.0 37.0 37.0 130-134 35.318850000000005 37.0 37.0 37.0 34.6 37.0 135-139 35.3197 37.0 37.0 37.0 32.2 37.0 140-144 35.22045000000001 37.0 37.0 37.0 27.4 37.0 145-149 35.109750000000005 37.0 37.0 37.0 27.4 37.0 150-151 34.848625 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 6.0 14 3.0 15 3.0 16 0.0 17 2.0 18 1.0 19 1.0 20 0.0 21 3.0 22 5.0 23 9.0 24 5.0 25 8.0 26 11.0 27 19.0 28 14.0 29 27.0 30 32.0 31 50.0 32 69.0 33 117.0 34 230.0 35 698.0 36 2534.0 37 152.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.57863054928518 22.648607975921745 12.515675946827187 25.257085527965888 2 27.450000000000003 25.224999999999998 30.7 16.625 3 22.95 27.875 31.275 17.9 4 24.325 34.9 21.6 19.175 5 26.450000000000003 35.425000000000004 23.575 14.549999999999999 6 21.0 39.2 23.05 16.75 7 22.275 19.625 38.824999999999996 19.275000000000002 8 20.325 25.174999999999997 30.4 24.099999999999998 9 22.675 25.95 29.825000000000003 21.55 10-14 23.515 29.360000000000003 26.595000000000002 20.53 15-19 23.105 28.58 27.87 20.445 20-24 22.660665166291576 28.162040510127532 28.657164291072768 20.520130032508128 25-29 21.635408852213054 29.202300575143784 28.497124281070267 20.66516629157289 30-34 22.93573393348337 28.797199299824953 27.82195548887222 20.445111277819457 35-39 22.570642660665165 28.377094273568392 28.75718929732433 20.29507376844211 40-44 21.770442610652662 28.927231807951987 28.377094273568392 20.92523130782696 45-49 23.035758939734936 28.312078019504877 28.152038009502377 20.500125031257816 50-54 22.660665166291576 28.35708927231808 28.207051762940733 20.775193798449614 55-59 22.780695173793447 28.40710177544386 27.9869967491873 20.825206301575395 60-64 23.330832708177045 27.301825456364092 29.112278069517377 20.255063765941486 65-69 22.713407011051657 28.4742711406711 28.059208881332196 20.753112966945043 70-74 22.140535133783445 28.902225556389098 28.422105526381596 20.53513378344586 75-79 23.435858964741186 28.507126781695426 28.13703425856464 19.919979994998748 80-84 23.055763940985248 28.192048012003003 27.711927981995498 21.040260065016252 85-89 23.0207551887972 28.367091772943237 27.73693423355839 20.875218804701177 90-94 23.360840210052515 27.981995498874717 27.911977994498628 20.745186296574143 95-99 22.790697674418606 29.147286821705425 27.71692923230808 20.34508627156789 100-104 23.16079019754939 28.30207551887972 27.731932983245812 20.80520130032508 105-109 24.056014003500874 28.257064266066518 26.881720430107524 20.80520130032508 110-114 23.20080020005001 28.912228057014254 27.376844211052763 20.510127531882972 115-119 23.07076769192298 28.962240560140035 27.916979244811202 20.05001250312578 120-124 23.93098274568642 28.432108027006752 28.052013003250813 19.584896224056013 125-129 23.785946486621658 28.172043010752688 27.141785446361588 20.900225056264066 130-134 24.34352023208123 27.89476316710849 28.054819186715353 19.706897414094936 135-139 24.307292187656294 27.598279483845158 27.98839651895569 20.106031809542863 140-144 25.081270317579396 27.741935483870968 27.516879219804952 19.659914978744688 145-149 25.1262815703926 28.95723930982746 26.89672418104526 19.019754938734685 150-151 24.284106539952482 28.09803676378642 26.697511566837562 20.920345129423534 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 0.0 14 1.0 15 1.0 16 1.0 17 1.0 18 0.0 19 1.0 20 1.5 21 2.5 22 5.0 23 5.0 24 3.5 25 4.5 26 6.5 27 7.5 28 12.5 29 21.0 30 25.5 31 27.5 32 28.5 33 38.0 34 59.0 35 84.0 36 97.0 37 111.5 38 152.5 39 188.5 40 216.5 41 241.5 42 264.0 43 278.5 44 291.0 45 279.0 46 237.0 47 218.5 48 198.5 49 171.0 50 160.0 51 132.5 52 95.5 53 72.5 54 54.5 55 47.5 56 38.5 57 26.5 58 20.5 59 17.5 60 14.5 61 9.0 62 5.0 63 2.5 64 1.0 65 0.5 66 0.0 67 0.0 68 0.0 69 1.0 70 2.0 71 1.5 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.5 87 1.0 88 1.5 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 0.5 97 0.5 98 1.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.325 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.015 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.034999999999999996 135-139 0.03 140-144 0.025 145-149 0.025 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.92500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 72.25942897426859 51.24999999999999 2 19.245682058512514 27.3 3 5.428269298554811 11.55 4 1.7624250969333801 5.0 5 0.9517095523440254 3.375 6 0.31723651744800846 1.35 7 0.035248501938667604 0.17500000000000002 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAGGATTAGACTTGAAGAGGTACATGGGAAGATGGTACGAAATTGCTTC 7 0.17500000000000002 No Hit GCCCACATATCCATTTTCTTGGTTGGATTCTTGACAGGGAATTGGATCGG 6 0.15 No Hit GTTTGGGAAATATTACCGAGAACCAAAGAAGTCAGGCCCTCTGCCTTTTC 6 0.15 No Hit CGGAAAGCAGAAGATGATTTGAGATTTTTTCTGCAGGTGGTGTTGTAAGC 6 0.15 No Hit CTGAGCTTGAGTGCATTCTTCTAAGTAAAAGAAATCCCTTAATTTCATCA 6 0.15 No Hit TTTGAGGAAAGGATTTATACTGCCTCCACCAATCAGTCTGATTATCTGCG 6 0.15 No Hit AAGCAGTTACAGAATCCGCTGAACGTGTTGCTGGATGTAGACATGGCTGA 6 0.15 No Hit ATCAAGCAAGCTGCTGATCTTGAGGCCTCTAAGCTCCCTCAACAGTCTTC 6 0.15 No Hit CCAGCATTTGCCATCACTGGAGTGAATAATCCTGAAGATTTGACATCGAT 6 0.15 No Hit GAGGCAGTCAAGGCTAACTTCAATGATAAGTTTGATGAGCACCGAAAGAA 6 0.15 No Hit AGGGGACCCTCAGCTTTCAGGATTAGAAGATTTCAGAGTAAAGATTCGAG 5 0.125 No Hit GTTTGCACCTCTAGATCTACCCTGAACACATATTAGTGCATCTAATTTTG 5 0.125 No Hit CTGAGCTTGACTAAGCTTAAATATGGAGCTTAAGCTCTTGTTATCCTCTT 5 0.125 No Hit GATGATGAGGTTTTGGGGTCACAGAACATTTGCTGTTACACTCATAGGGA 5 0.125 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit ATCTTTCCATTTTCTCTCTTACACGAGACATCATGGATCCTTACAAGTAT 5 0.125 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 5 0.125 No Hit CGATCCAAAAAATAAGAAATCCCTATCGATGCACGTTATTATTAATTTAT 5 0.125 No Hit AGAAAGTAATGAACATAATAAAGCCAAAGCCAAATCCACAGCAACAACTC 5 0.125 No Hit GGAAAGACAATAGCTTTACTATCATTAATCACAAGCTACACTATCTCTAA 5 0.125 No Hit CAATGTTGCAATTTGTGAAGCTGACTCCAAGGTATTCTCTCTTTTATAAC 5 0.125 No Hit CAACATCTTATATCCATTCCCGCATAATAATTTTCTTTTAAACACAGGAA 5 0.125 No Hit CCACAAACAAAACCACTATCCTTGAAAGACGACTGAAACAAATCCGTAAC 5 0.125 No Hit AGAGGACATAATCGTCCGCAAAATCCTCTTAATCTCTCTCACCGATTCCT 5 0.125 No Hit AACATCCAGAAGGAGTCCACTCTCCACTTGGTTCTTCGGTTGAGGGGTGG 5 0.125 No Hit GCCATTTTATCAAATCTTGATGATCACTCTAGTGTTAATCACATGTTAAC 5 0.125 No Hit TGAAGGTTGGGGCTTATAGAGCCATAGATATACTTGAGGTATCAGCTGGC 5 0.125 No Hit GGTATTGCTCCTTTCCGATCATTCTTGTGGAAAATGTTCTTCGAGAAGCA 5 0.125 No Hit GCAAACCCTAACACTAACTCAAATCTCCACTCTCACTTCAATAAATCAAA 5 0.125 No Hit ATTTTGATCAAGAAGTACGAAATGAAAAACGGCGACAGTTGTTGTCAAAT 5 0.125 No Hit GTGGACTCTCCAAAGAGCCTTGAAAATGGGGAGGGTGGGAGGCTGATAGC 5 0.125 No Hit CTCAGGTTCTCAATTGCCTAGACCTTTTCTCTACAAGCTTCTTTCCATGG 5 0.125 No Hit TGGTGATCAAAAGCTCAGCTGAAGTTCAAGAATCAAACTATGACAAAGAA 5 0.125 No Hit GTGGAACCTGAACAACGAGTGCCAGATTTTATCAAGGCTGGAGCTGATAT 5 0.125 No Hit AAGAAACTGCTGGTTCTCAATTCTCAAACAATCAAAAACACCAGCTCAGA 5 0.125 No Hit GGAAGAAGAAATGGAACCCAAAGTTTATGTCTCAACAGAAAACACACCAC 5 0.125 No Hit ATGAGCTTGTCAAGACCGCGAAAACCATTGCATCTCCTGGTCGTGGTATT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.1 0.0 0.0 0.0 0.0 84-85 0.125 0.0 0.0 0.0 0.0 86-87 0.125 0.0 0.0 0.0 0.0 88-89 0.125 0.0 0.0 0.0 0.0 90-91 0.1875 0.0 0.0 0.0 0.0 92-93 0.2375 0.0 0.0 0.0 0.0 94-95 0.25 0.0 0.0 0.0 0.0 96-97 0.35 0.0 0.0 0.0 0.0 98-99 0.5375 0.0 0.0 0.0 0.0 100-101 0.6 0.0 0.0 0.0 0.0 102-103 0.7875 0.0 0.0 0.0 0.0 104-105 0.9125000000000001 0.0 0.0 0.0 0.0 106-107 0.9375 0.0 0.0 0.0 0.0 108-109 1.0499999999999998 0.0 0.0 0.0 0.0 110-111 1.225 0.0 0.0 0.0 0.0 112-113 1.4375 0.0 0.0 0.0 0.0 114-115 1.55 0.0 0.0 0.0 0.0 116-117 1.7625000000000002 0.0 0.0 0.0 0.0 118-119 2.0125 0.0 0.0 0.0 0.0 120-121 2.35 0.0 0.0 0.0 0.0 122-123 2.8 0.0 0.0 0.0 0.0 124-125 3.0625 0.0 0.0 0.0 0.0 126-127 3.4124999999999996 0.0 0.0 0.0 0.0 128-129 3.7125 0.0 0.0 0.0 0.0 130-131 4.0625 0.0 0.0 0.0 0.0 132-133 4.6875 0.0 0.0 0.0 0.0 134-135 4.8625 0.0 0.0 0.0 0.0 136-137 5.35 0.0 0.0 0.0 0.0 138-139 6.0375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCACT 10 0.006830828 145.0 145 >>END_MODULE Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830747 spots for SRR13695495.sra Written 830747 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra Read 830736 spots for SRR13695495.sra Written 830736 spots for SRR13695495.sra SRR ids: ['SRR13695495.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_1207dr6n SRR13695495.sra spots: 16614731 blocks: [[1, 830736], [830737, 1661472], [1661473, 2492208], [2492209, 3322944], [3322945, 4153680], [4153681, 4984416], [4984417, 5815152], [5815153, 6645888], [6645889, 7476624], [7476625, 8307360], [8307361, 9138096], [9138097, 9968832], [9968833, 10799568], [10799569, 11630304], [11630305, 12461040], [12461041, 13291776], [13291777, 14122512], [14122513, 14953248], [14953249, 15783984], [15783985, 16614731]] SRR13695495 file size 5624712 SRR13695495 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695495 SRR13695495_1.fastq SRR13695495_2.fastq Input file: SRR13695495_1.fastq Paired file: SRR13695495_2.fastq trimmed: SRR13695495-trimmed-pair1.fastq, SRR13695495-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 07:52:54 2025 >> started Wed Feb 12 07:53:11 2025 >> done (17.035s) 16614731 read pairs processed; of these: 109 ( 0.00%) short read pairs filtered out after trimming by size control 1006 ( 0.01%) empty read pairs filtered out after trimming by size control 16613616 (99.99%) read pairs available; of these: 1439992 ( 8.67%) trimmed read pairs available after processing 15173624 (91.33%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 2 0.00% 20 2 0.00% 21 2 0.00% 22 2 0.00% 23 0 0.00% 24 3 0.00% 25 2 0.00% 26 2 0.00% 27 1 0.00% 28 2 0.00% 29 5 0.00% 30 4 0.00% 31 5 0.00% 32 6 0.00% 33 4 0.00% 34 4 0.00% 35 5 0.00% 36 7 0.00% 37 2 0.00% 38 4 0.00% 39 8 0.00% 40 8 0.00% 41 12 0.00% 42 3 0.00% 43 8 0.00% 44 6 0.00% 45 11 0.00% 46 10 0.00% 47 13 0.00% 48 10 0.00% 49 25 0.00% 50 25 0.00% 51 32 0.00% 52 27 0.00% 53 29 0.00% 54 22 0.00% 55 36 0.00% 56 40 0.00% 57 53 0.00% 58 66 0.00% 59 65 0.00% 60 82 0.00% 61 90 0.00% 62 99 0.00% 63 122 0.00% 64 137 0.00% 65 186 0.00% 66 184 0.00% 67 202 0.00% 68 283 0.00% 69 298 0.00% 70 340 0.00% 71 419 0.00% 72 486 0.00% 73 525 0.00% 74 666 0.00% 75 694 0.00% 76 836 0.01% 77 856 0.01% 78 999 0.01% 79 1147 0.01% 80 1226 0.01% 81 1479 0.01% 82 1709 0.01% 83 1954 0.01% 84 2337 0.01% 85 2626 0.02% 86 2674 0.02% 87 2972 0.02% 88 3212 0.02% 89 3508 0.02% 90 3925 0.02% 91 4169 0.03% 92 4518 0.03% 93 5211 0.03% 94 5697 0.03% 95 6331 0.04% 96 6536 0.04% 97 7124 0.04% 98 7498 0.05% 99 7929 0.05% 100 8478 0.05% 101 8795 0.05% 102 9529 0.06% 103 10313 0.06% 104 10828 0.07% 105 11568 0.07% 106 12081 0.07% 107 13003 0.08% 108 13423 0.08% 109 14160 0.09% 110 14639 0.09% 111 15385 0.09% 112 16000 0.10% 113 16672 0.10% 114 17358 0.10% 115 18538 0.11% 116 19174 0.12% 117 20108 0.12% 118 20714 0.12% 119 21167 0.13% 120 22358 0.13% 121 22872 0.14% 122 22717 0.14% 123 24204 0.15% 124 25252 0.15% 125 25971 0.16% 126 26500 0.16% 127 27485 0.17% 128 28480 0.17% 129 29234 0.18% 130 30287 0.18% 131 30608 0.18% 132 31242 0.19% 133 32379 0.19% 134 32968 0.20% 135 33506 0.20% 136 34771 0.21% 137 35275 0.21% 138 36620 0.22% 139 38044 0.23% 140 38276 0.23% 141 39155 0.24% 142 39948 0.24% 143 39867 0.24% 144 41103 0.25% 145 42077 0.25% 146 43193 0.26% 147 43946 0.26% 148 45223 0.27% 149 46319 0.28% 150 46320 0.28% 151 15173624 91.33% 16613616 reads passed initial QC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=23 prefix-density=0.26 prefix-fanout=2.0 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=28 fanout-score=549.73 fanout-score-rank=1 prefix-density=0.23 prefix-fanout=17.5 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTG criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=2.40 fanout-score-rank=26 prefix-density=0.39 prefix-fanout=2.3 sequence=TGCAAGTGCGGCAGTGGCTGCAATGGATGCAGCATGTACCCAGACTTGAGTTTCTCCGAGACCACCACAAGTCAGACAATCATTGCTGGTGTAGCTCCAGTTAGGATGTTCTACGAGAGCTCTGAGATGAACTTTGGTGCTGAGAATGGCTGCAA criterion=fanout-score sequence-density=0.07 sequence-density-rank=27 fanout-score=33.43 fanout-score-rank=1 prefix-density=0.22 prefix-fanout=10.4 sequence=AAGGCCAAGATCCAGGACAAGGA SRR13695495 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 07:53:53 Started mapping on | Feb 12 07:53:53 Finished on | Feb 12 07:56:10 Mapping speed, Million of reads per hour | 436.56 Number of input reads | 16613616 Average input read length | 298 UNIQUE READS: Uniquely mapped reads number | 15432096 Uniquely mapped reads % | 92.89% Average mapped length | 296.58 Number of splices: Total | 15259028 Number of splices: Annotated (sjdb) | 14876915 Number of splices: GT/AG | 14958477 Number of splices: GC/AG | 228638 Number of splices: AT/AC | 9739 Number of splices: Non-canonical | 62174 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 3.00 Insertion rate per base | 0.02% Insertion average length | 2.42 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 403468 % of reads mapped to multiple loci | 2.43% Number of reads mapped to too many loci | 75420 % of reads mapped to too many loci | 0.45% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.05% % of reads unmapped: other | 0.18% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 778254 778254 778254 N_multimapping 403468 403468 403468 N_noFeature 684672 15035764 932060 N_ambiguous 258818 1923 108497 UnstrandedReadsAssigned:14488606 PositiveStrandReadsAssigned:394409 NegativeStrandReadsAssigned:14391539 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695495 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695495-trimmed-pair1.fastq SRR13695495-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,613,616 reads, 14,407,768 reads pseudoaligned [quant] estimated average fragment length: 262.03 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,064 rounds 52401 SRR13695495.ke.tsv 34699 SRR13695495.se.tsv 87100 total ==> SRR13695495.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1756.97 886 30.7964 Potri.005G024800.1.v4.1 1035 773.97 464 36.612 Potri.004G059700.1.v4.1 961 700.094 6 0.52339 Potri.007G009000.2.v4.1 1416 1154.97 0 0 Potri.003G141000.2.v4.1 2943 2681.97 854 19.4462 Potri.016G087400.1.v4.1 270 78.8136 820 635.393 Potri.015G069301.1.v4.1 564 314.229 0 0 Potri.010G195200.1.v4.1 1773 1511.97 206 8.32059 Potri.012G127500.1.v4.1 977 716.024 64 5.45861 ==> SRR13695495.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 177 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 200 Potri.001G212900.v4.1 10 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 8 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 20 SRR13695495 completed mapping pipeline successfully