Starting /dee2/code/volunteer_pipeline.sh SRR13695496 current disk space = 3049920438272 free memory = 1373494676 SRR13695496 SRAfilesize 17d11ce426eb041e9534cc8bd3983ba8 SRR13695496.sra SRR13695496.sra file validated SRR13695496 is paired end SRR13695496 is conventional basespace SRR13695496 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695496_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.599 37.0 37.0 37.0 37.0 37.0 2 36.395 37.0 37.0 37.0 37.0 37.0 3 36.5415 37.0 37.0 37.0 37.0 37.0 4 36.4995 37.0 37.0 37.0 37.0 37.0 5 36.596 37.0 37.0 37.0 37.0 37.0 6 36.571 37.0 37.0 37.0 37.0 37.0 7 36.4655 37.0 37.0 37.0 37.0 37.0 8 36.556 37.0 37.0 37.0 37.0 37.0 9 36.622 37.0 37.0 37.0 37.0 37.0 10-14 36.5616 37.0 37.0 37.0 37.0 37.0 15-19 36.5471 37.0 37.0 37.0 37.0 37.0 20-24 36.5448 37.0 37.0 37.0 37.0 37.0 25-29 36.4755 37.0 37.0 37.0 37.0 37.0 30-34 36.46679999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.417100000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.435500000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.339600000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.336200000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.3565 37.0 37.0 37.0 37.0 37.0 60-64 36.346900000000005 37.0 37.0 37.0 37.0 37.0 65-69 36.3292 37.0 37.0 37.0 37.0 37.0 70-74 36.304700000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.28179999999999 37.0 37.0 37.0 37.0 37.0 80-84 36.2906 37.0 37.0 37.0 37.0 37.0 85-89 36.217 37.0 37.0 37.0 37.0 37.0 90-94 36.1982 37.0 37.0 37.0 37.0 37.0 95-99 36.1947 37.0 37.0 37.0 37.0 37.0 100-104 36.10979999999999 37.0 37.0 37.0 37.0 37.0 105-109 36.1194 37.0 37.0 37.0 37.0 37.0 110-114 36.082 37.0 37.0 37.0 37.0 37.0 115-119 36.1154 37.0 37.0 37.0 37.0 37.0 120-124 36.0054 37.0 37.0 37.0 37.0 37.0 125-129 35.947500000000005 37.0 37.0 37.0 37.0 37.0 130-134 35.962599999999995 37.0 37.0 37.0 37.0 37.0 135-139 35.9622 37.0 37.0 37.0 37.0 37.0 140-144 35.8437 37.0 37.0 37.0 37.0 37.0 145-149 35.608799999999995 37.0 37.0 37.0 37.0 37.0 150-151 35.485749999999996 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 2.0 24 4.0 25 3.0 26 6.0 27 10.0 28 9.0 29 21.0 30 27.0 31 40.0 32 50.0 33 63.0 34 105.0 35 320.0 36 2958.0 37 382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.025 12.225 7.3999999999999995 36.35 2 19.503012048192772 13.60441767068273 34.713855421686745 32.178714859437754 3 17.1 18.375 26.674999999999997 37.85 4 22.225 23.875 25.074999999999996 28.825 5 22.625 29.75 23.95 23.674999999999997 6 21.975 33.4 22.375 22.25 7 15.35 29.775000000000002 38.475 16.400000000000002 8 17.5 28.725 31.2 22.575 9 17.65 24.224999999999998 34.525 23.599999999999998 10-14 19.165 29.86 28.57 22.405 15-19 19.825 27.575 28.29 24.310000000000002 20-24 20.05 29.07 27.700000000000003 23.18 25-29 19.655 28.835 28.194999999999997 23.315 30-34 19.33 28.005000000000003 28.055000000000003 24.610000000000003 35-39 19.564999999999998 29.110000000000003 27.689999999999998 23.635 40-44 20.255000000000003 28.565 27.884999999999998 23.294999999999998 45-49 20.22 28.794999999999998 27.685 23.3 50-54 20.26 27.689999999999998 28.525 23.525 55-59 20.885 28.68 27.71 22.725 60-64 20.06 28.305000000000003 27.805000000000003 23.830000000000002 65-69 20.085 27.855 27.834999999999997 24.224999999999998 70-74 20.1 28.665000000000003 27.639999999999997 23.595 75-79 19.96 28.465 28.365000000000002 23.21 80-84 20.715 27.589999999999996 27.744999999999997 23.95 85-89 20.44 28.49 27.375 23.695 90-94 20.525 28.595 27.355 23.525 95-99 20.669999999999998 28.98 27.255000000000003 23.095 100-104 21.065 28.994999999999997 26.82 23.119999999999997 105-109 20.32 27.555000000000003 27.98 24.145 110-114 21.560000000000002 28.46 26.85 23.13 115-119 21.525 27.805000000000003 27.105 23.565 120-124 20.355 27.67 27.634999999999998 24.34 125-129 21.404999999999998 28.115000000000002 26.590000000000003 23.89 130-134 20.66 27.555000000000003 27.334999999999997 24.45 135-139 21.59 28.449999999999996 26.200000000000003 23.76 140-144 21.775 26.99 27.439999999999998 23.794999999999998 145-149 21.6 27.74 26.41 24.25 150-151 21.2625 27.6 27.2625 23.875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.5 5 0.5 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.0 18 1.0 19 0.0 20 1.0 21 1.0 22 2.5 23 4.5 24 4.0 25 5.0 26 5.5 27 5.5 28 5.5 29 11.0 30 19.0 31 22.0 32 25.5 33 41.0 34 63.5 35 76.5 36 83.0 37 97.0 38 116.5 39 162.0 40 192.5 41 206.0 42 236.0 43 268.5 44 272.0 45 268.5 46 280.5 47 258.0 48 226.0 49 211.0 50 186.5 51 147.0 52 112.0 53 94.0 54 69.5 55 50.5 56 49.5 57 32.5 58 24.5 59 18.5 60 12.5 61 10.5 62 6.5 63 4.5 64 2.5 65 2.0 66 0.5 67 0.0 68 0.0 69 0.0 70 0.5 71 0.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.675 #Duplication Level Percentage of deduplicated Percentage of total 1 69.4126339120798 46.975 2 19.985223494643517 27.05 3 6.723309937199852 13.65 4 2.4011821204285186 6.5 5 0.8496490579977836 2.875 6 0.3694126339120798 1.5 7 0.07388252678241596 0.35000000000000003 8 0.07388252678241596 0.4 9 0.07388252678241596 0.44999999999999996 >10 0.03694126339120798 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGCACCAATCAGCTTCCTCAGCTCCTCTCCCTGAGCTGTAGTAATAGGA 10 0.25 No Hit CCCAGCACACATCAATCATATTAGGCCTGTCTGCTCCTTGTGGCTTCAAA 9 0.22499999999999998 No Hit CTCAAGCTATAATTATGCTGATAATAAAAAATTGCATACAGGTCCTAGAT 9 0.22499999999999998 No Hit CCCAAGCAAGAACATCAACAATGTTGAAACCAACTGGATCATTTGATTTC 8 0.2 No Hit CGGAAGAACAAGAAGAGAAGACAAAGAAAAGAGAATCACGTTTTTTTTTT 8 0.2 No Hit CTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAAC 7 0.17500000000000002 No Hit GGCAGCTGTGTGTACATATATACATCTAATGAAGACACAGATCAAGGTTT 7 0.17500000000000002 No Hit CTTCTGGAAAACAGCATCTATTGGGACTTGCTTCTCAAAGAAACCATAAG 6 0.15 No Hit CTAGAAACAGTAGTTGGGAACTTGGGATAGGCAAATCAGAGCAGCAGAAC 6 0.15 No Hit AGCTCATCTACTCTTCATCCTCATCGGTCACGAATTTCCCAGTCCCAGGA 6 0.15 No Hit GCTTCTCTTTCTATAGGCCAGGGTACATTAGAAAGGAGAGAGCTGTTACT 6 0.15 No Hit GGTAGTTTCCACATAGTCCAGTAGCGTCCATCATAGTACCCTGGGGACTG 6 0.15 No Hit GGTGTCAGAACTCTCCACCTCCAAGGTGATTGTCTTGCCAGTAAGGGTCT 6 0.15 No Hit CCGGCATCCTCACCGTGGTAATTTGAGTTCCCTGATAAACCAATGTTTGC 6 0.15 No Hit GCCTGATATTCAAGCAATGGCCTCACACACTTAGGCTTGCAAGCTTCCTC 6 0.15 No Hit GACTACTTCTGTGCACTTTATCGGGAATGCATACCTGAGCAGGACAGATT 6 0.15 No Hit CTAGCAAGCTGAGATTTTGCGGATAAAATTTCTCCAAATTCCTTCAATTG 6 0.15 No Hit GAGAGAAGGATGTCAGCTGGTAGTTTGAACTTTGAAAGATAGTGGGGCTT 5 0.125 No Hit TTCCATCCTTTAGGAATTGTATAGCCTTTTACATTGATATCTGTCATCGT 5 0.125 No Hit ATCTGGTATAAACGAAACTCCAAACAGAAAATATTAAGTGGGGATTAAGG 5 0.125 No Hit GGCATGAATACTTGTTCATCAGGAACTTTATTTACAAGGATCTGTCCAGA 5 0.125 No Hit GCCGTAGTCTTTCTTTGTAGTAAAGGCAGTCTTGTTCTCCAAGAGATTAT 5 0.125 No Hit AAGCAGCTGGCCAGTAAGGTTTTGTTGCTCATGAAGAAAACGGGACTGGA 5 0.125 No Hit TTCTGCAGCACTATTTCATAGACAGAAGCTACCCTCTGATTGCAATAACT 5 0.125 No Hit CAGCTAAAACAAGGAAACCATAGCAGCGCAGATTTTAAACTGAATGACAC 5 0.125 No Hit GCGCAAGTTTCAATTCATGAGCTCGTTACATCACAAGTTAACATTACAAG 5 0.125 No Hit GAAAGGATTTATGAACAAAGGTCGGAGGATATGTCTAAGCTTTGATACAA 5 0.125 No Hit GCTATTAATGGATTACTTGGAAGTTAATGATGTCAATCCTAAATCAATCA 5 0.125 No Hit CGGTGGTGCGGGTGAGTAGCCTATGTTTTATAATTACATCATCTTCGAGC 5 0.125 No Hit TGCAAATTTAACCTCGTTGCTAATGTCCAAGTAGATACTGAAGTCCAAGA 5 0.125 No Hit AGCAGCTGTATTGGATCAGTTTTCAAGAAATTTCTCTGGACTCTGTGTCC 5 0.125 No Hit GTGCAGGCCATCTAGATCCAAGTGATGCTCCCTGAGTCTCTTCAGGTTAT 5 0.125 No Hit GGGGCATATCCATTCCCAATTTGACCAGGGTGGTTCATGATGATGACCTG 5 0.125 No Hit GCTATAAATAAACTTACAGGGATTCTCTGCTTCAAGTAGTTGCCAATTCT 5 0.125 No Hit GGTATGCTTGGATGCTAAGGACTTGGGGCCATTTCGATATATACGGAGAT 5 0.125 No Hit CATCAGTCAAATTTAAGAGCTTCTTTGTAATACAAACAGATTGATATACT 5 0.125 No Hit GCATCCTTAAGTAATGGAACGGGGGGTTTGTCTTCACCAGGTTGAGGATA 5 0.125 No Hit GTTGAAAGATCAACAGAAGAGTTAATTAGAGTTTTCATGGATTAAATTTG 5 0.125 No Hit GGAAGATAAAACTCCCGCACACCATATTTCAGACAGAGCCTTCAAAAACA 5 0.125 No Hit GTGGCCACTCATAGCTTCTCGTTACTCCCCTGCTCCGATTAGAAACAGAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.1 0.0 0.0 0.0 0.0 64-65 0.1375 0.0 0.0 0.0 0.0 66-67 0.175 0.0 0.0 0.0 0.0 68-69 0.175 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.1875 0.0 0.0 0.0 0.0 76-77 0.21250000000000002 0.0 0.0 0.0 0.0 78-79 0.2375 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.4 0.0 0.0 0.0 0.0 84-85 0.475 0.0 0.0 0.0 0.0 86-87 0.4875 0.0 0.0 0.0 0.0 88-89 0.65 0.0 0.0 0.0 0.0 90-91 0.8 0.0 0.0 0.0 0.0 92-93 0.8875 0.0 0.0 0.0 0.0 94-95 1.025 0.0 0.0 0.0 0.0 96-97 1.1625 0.0 0.0 0.0 0.0 98-99 1.3625 0.0 0.0 0.0 0.0 100-101 1.7125 0.0 0.0 0.0 0.0 102-103 2.0625 0.0 0.0 0.0 0.0 104-105 2.375 0.0 0.0 0.0 0.0 106-107 2.6125 0.0 0.0 0.0 0.0 108-109 2.8875 0.0 0.0 0.0 0.0 110-111 3.075 0.0 0.0 0.0 0.0 112-113 3.4749999999999996 0.0 0.0 0.0 0.0 114-115 3.8625 0.0 0.0 0.0 0.0 116-117 4.225 0.0 0.0 0.0 0.0 118-119 4.625 0.0 0.0 0.0 0.0 120-121 5.0 0.0 0.0 0.0 0.0 122-123 5.4125 0.0 0.0 0.0 0.0 124-125 6.0 0.0 0.0 0.0 0.0 126-127 6.4625 0.0 0.0 0.0 0.0 128-129 7.2375 0.0 0.0 0.0 0.0 130-131 7.6875 0.0 0.0 0.0 0.0 132-133 8.25 0.0 0.0 0.0 0.0 134-135 8.8875 0.0 0.0 0.0 0.0 136-137 9.25 0.0 0.0 0.0 0.0 138-139 9.912500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13695496 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695496_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.06975 37.0 37.0 37.0 37.0 37.0 2 36.135 37.0 37.0 37.0 37.0 37.0 3 36.0535 37.0 37.0 37.0 37.0 37.0 4 36.1695 37.0 37.0 37.0 37.0 37.0 5 36.1515 37.0 37.0 37.0 37.0 37.0 6 36.321 37.0 37.0 37.0 37.0 37.0 7 36.1895 37.0 37.0 37.0 37.0 37.0 8 36.3285 37.0 37.0 37.0 37.0 37.0 9 36.266 37.0 37.0 37.0 37.0 37.0 10-14 36.159499999999994 37.0 37.0 37.0 37.0 37.0 15-19 36.158 37.0 37.0 37.0 37.0 37.0 20-24 36.109500000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.093 37.0 37.0 37.0 37.0 37.0 30-34 36.116699999999994 37.0 37.0 37.0 37.0 37.0 35-39 36.04600000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.037 37.0 37.0 37.0 37.0 37.0 45-49 36.041599999999995 37.0 37.0 37.0 37.0 37.0 50-54 36.0276 37.0 37.0 37.0 37.0 37.0 55-59 35.9764 37.0 37.0 37.0 37.0 37.0 60-64 35.9415 37.0 37.0 37.0 37.0 37.0 65-69 35.9403 37.0 37.0 37.0 37.0 37.0 70-74 35.86880000000001 37.0 37.0 37.0 37.0 37.0 75-79 35.8375 37.0 37.0 37.0 37.0 37.0 80-84 35.859399999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.7611 37.0 37.0 37.0 37.0 37.0 90-94 35.7364 37.0 37.0 37.0 37.0 37.0 95-99 35.7933 37.0 37.0 37.0 37.0 37.0 100-104 35.7106 37.0 37.0 37.0 37.0 37.0 105-109 35.729200000000006 37.0 37.0 37.0 37.0 37.0 110-114 35.653400000000005 37.0 37.0 37.0 37.0 37.0 115-119 35.624 37.0 37.0 37.0 37.0 37.0 120-124 35.518499999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.5346 37.0 37.0 37.0 37.0 37.0 130-134 35.3788 37.0 37.0 37.0 34.6 37.0 135-139 35.41545 37.0 37.0 37.0 37.0 37.0 140-144 35.245400000000004 37.0 37.0 37.0 32.2 37.0 145-149 35.104049999999994 37.0 37.0 37.0 27.4 37.0 150-151 34.723 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 2.0 13 7.0 14 3.0 15 2.0 16 1.0 17 1.0 18 2.0 19 2.0 20 3.0 21 0.0 22 5.0 23 0.0 24 10.0 25 6.0 26 3.0 27 14.0 28 18.0 29 9.0 30 27.0 31 48.0 32 56.0 33 99.0 34 232.0 35 568.0 36 2695.0 37 185.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.44118384750439 20.265864058189116 11.286681715575622 25.00627037873088 2 27.650000000000002 25.650000000000002 29.25 17.45 3 20.275000000000002 27.0 33.375 19.35 4 26.150000000000002 31.55 23.1 19.2 5 25.525 35.5 21.525 17.45 6 21.75 38.824999999999996 22.825 16.6 7 23.5 23.225 34.599999999999994 18.675 8 20.424999999999997 27.650000000000002 25.8 26.125 9 22.6 25.874999999999996 29.45 22.075 10-14 25.05 29.830000000000002 25.28 19.84 15-19 23.565 28.634999999999998 27.125 20.674999999999997 20-24 23.809285571342805 28.46708024814889 26.971182709625772 20.75245147088253 25-29 23.813813813813812 28.86886886886887 26.996996996996998 20.32032032032032 30-34 22.951065746022216 28.76513559491644 27.629340538376862 20.65445812068448 35-39 23.809285571342805 28.707224334600763 26.535921552931757 20.947568541124674 40-44 23.491444010807566 28.760132092464723 26.943860702491744 20.804563194235964 45-49 22.863718230938563 28.667200320192116 28.417050230138084 20.05203121873124 50-54 23.376688344172088 27.5687843921961 27.913956978489246 21.14057028514257 55-59 23.408408408408405 28.66866866866867 27.087087087087085 20.835835835835837 60-64 24.05943566139684 27.71662997798679 26.92615569341605 21.29777866720032 65-69 23.544417767106843 27.78111244497799 27.516006402561022 21.158463385354143 70-74 23.55855855855856 27.81781781781782 26.63163163163163 21.99199199199199 75-79 23.663931144915935 27.336869495596478 27.171737389911932 21.82746196957566 80-84 23.486743371685844 28.07903951975988 27.233616808404204 21.200600300150075 85-89 23.566209588629768 27.06435792212992 27.825042538284457 21.54438995095586 90-94 24.08445067040224 27.146287772663598 27.52651590954573 21.242745647388432 95-99 23.911955977988995 27.448724362181093 28.279139569784896 20.36018009004502 100-104 24.744744744744747 27.73773773773774 26.476476476476474 21.04104104104104 105-109 24.532078870983888 28.215393854469024 27.119407466720048 20.133119807827043 110-114 24.15949569741845 27.966780068040826 27.28637182309386 20.58735241144687 115-119 24.499599679743795 28.532826261008807 26.531224979983985 20.43634907926341 120-124 25.285285285285287 28.483483483483486 26.54154154154154 19.68968968968969 125-129 25.590472377902323 28.05244195356285 26.80144115292234 19.55564451561249 130-134 24.727145288875537 27.776108941624113 27.555822569340144 19.940923200160206 135-139 25.55683467641023 28.264677911807397 26.417738625556836 19.760748786225538 140-144 26.53091855113068 27.126275765459273 26.891134680808488 19.45167100260156 145-149 26.98333249912408 27.593973672355975 26.072375994794534 19.350317833725413 150-151 28.492739108662995 26.952929394091136 26.164246369554334 18.39008512769154 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.5 7 1.0 8 0.5 9 0.5 10 1.0 11 1.0 12 0.5 13 0.0 14 0.5 15 1.0 16 1.5 17 1.0 18 0.0 19 0.5 20 1.0 21 0.5 22 2.5 23 4.0 24 5.5 25 6.0 26 3.0 27 1.5 28 3.0 29 6.0 30 7.0 31 10.0 32 17.0 33 22.5 34 32.5 35 48.5 36 66.0 37 75.5 38 113.5 39 158.5 40 180.0 41 224.0 42 268.5 43 308.5 44 315.0 45 308.0 46 269.0 47 231.5 48 228.0 49 207.0 50 162.5 51 127.5 52 133.5 53 110.5 54 82.0 55 59.5 56 41.5 57 38.0 58 31.5 59 25.0 60 15.5 61 6.0 62 3.0 63 5.0 64 5.5 65 4.0 66 1.5 67 0.5 68 0.5 69 1.5 70 1.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.5 98 1.0 99 1.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.325 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.06 25-29 0.1 30-34 0.06999999999999999 35-39 0.06 40-44 0.06999999999999999 45-49 0.06 50-54 0.05 55-59 0.1 60-64 0.06 65-69 0.04 70-74 0.1 75-79 0.08 80-84 0.05 85-89 0.09 90-94 0.06 95-99 0.05 100-104 0.1 105-109 0.09 110-114 0.06 115-119 0.08 120-124 0.1 125-129 0.08 130-134 0.13 135-139 0.105 140-144 0.06 145-149 0.105 150-151 0.15 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.25 #Duplication Level Percentage of deduplicated Percentage of total 1 70.73260073260073 48.275 2 19.047619047619047 26.0 3 6.446886446886448 13.200000000000001 4 2.271062271062271 6.2 5 0.7692307692307693 2.625 6 0.402930402930403 1.6500000000000001 7 0.07326007326007326 0.35000000000000003 8 0.07326007326007326 0.4 9 0.07326007326007326 0.44999999999999996 >10 0.10989010989010989 0.8500000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 14 0.35000000000000003 No Hit CAGTAGGAGATGGAGCTGTTGGTAAAACTTGTCTCTTGATCTCTTACACC 10 0.25 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 10 0.25 No Hit GGAGTTGATGCAGGTGAACTAAATTTCATTGGCATGGTCTGTGTTGTAGT 9 0.22499999999999998 No Hit ATTGGATTGAAGCTATTGATCCAAGGCATCGTTACGGGGCATTCTTGTAT 9 0.22499999999999998 No Hit TATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCCTCTGTG 8 0.2 No Hit CAGGGGTATCACTCTTTTTGGGAAGGTTTGTGTTCTTTAACTTCCAAAGG 8 0.2 No Hit GATTTAACAAAGAAACCATGAAGTACAAGAATTGAGCAACTGATTCTCTC 7 0.17500000000000002 No Hit GCTCTAGCACAGCTCAACATCGAGACAGGGATTCCAGTCAATGAAATTGA 7 0.17500000000000002 No Hit GGGAAGAAGAGCATCCAATCACAGCTTGAGAAGTTGAAGAAATATGCAAC 6 0.15 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 6 0.15 No Hit GCCAAAACATGATAAACTCTCTGATACCATCTCCGCGGAATGGACTGCCT 6 0.15 No Hit GTCAGAATGGAAGAAGAGCATTGATGAGATGTTGAATCAGCACCAAACAG 6 0.15 No Hit CTTGAGGTGGAAAGCTCTGACACTATTGATAATGTAAAGGCCAAGATCCA 6 0.15 No Hit GTTAATTTCACATATGGCACGTCAGGTAGATCGTCTCACAAGGATTGGAA 6 0.15 No Hit GTTGGGGGTAAGACTGGCTTGCTAATCTGGGCTGCTACCCTAGCTGGCCT 6 0.15 No Hit GGTTTTTGCTGGGGAGGGAACGTGGCAGTGAAATTAGCCAGTTCTAATGA 6 0.15 No Hit CTCACAAAATTCTCACTCCAGCTCTGGTTTTGAGAGTGTTGACATTCAAA 6 0.15 No Hit CTTCTCCAATATTTTAGGTCTTCTTCCTTTGTTCTTTGGATGTCGTTAGT 6 0.15 No Hit ACTGCAATCAATGCTTTTGTCCGGGGAATTGGAATCACTAGAAGTGATTG 6 0.15 No Hit CTTGCCAACTGTAAGAGCATGTTGGATGCCTTTAAAGCTGGTGGAGATTT 5 0.125 No Hit GCTGGGGATGGGCTGGAGTAATCTGGCTATTTAGCGTGGTGACATATGTG 5 0.125 No Hit AATTAAGATGTCAACAGAGGTGATGTTTCTTCGCAGTCACTGTTACCACG 5 0.125 No Hit GGCAGCTTCTCGGACGAGGAAATAGTGGATTTCTTGGTAGCTTTGCTTGT 5 0.125 No Hit CCAGTGGCGATAAGAGTCTCCTTTGTAGGGTTGGAATTCGTCTTCCTGAT 5 0.125 No Hit TGTTTCTTCCTCTGCGGCTTCTCTTACATTGTAGACTCCTAGTTCAAAGT 5 0.125 No Hit GTTCATTCGTCCAGAAGTATGCCGAACATATAACTTCGGTGAGCATGGCT 5 0.125 No Hit CCCTCCCCCTCCTCTCTTGCCCCAGCTGTCTTCATCGGCGGCACCTGTGT 5 0.125 No Hit ATGGGAAATCCTCAGTTTCTAATAGACGAAGCAGGGCTGCTGCTGTTCAT 5 0.125 No Hit TGCAAATATTGTGAGGGGTCTAATGGAAGGTGAGGAAGGGAAGAGGGTCC 5 0.125 No Hit GTGCTTACAAAAGCTTATTGATAGAATTGATTCTGGAGAGGAGTTACAAA 5 0.125 No Hit CTTATTCATCTTTGTTAGGCCATTATAACTTCGTTGTATTTTTGTTTAAT 5 0.125 No Hit GAAGGAAATGAGAGAAGGTTGACAGGAAAGCATGAGACAGCAAGCATTGA 5 0.125 No Hit AGAAATAAGGGTCTTTGATACGGCGAATACTCTGCCTCTCTTTTCGCTTC 5 0.125 No Hit GAAAGCTCAGGAGGTCCTCACGCAATTCTCAATTGAGCCACACAATTTTG 5 0.125 No Hit TGGCTGACGATCCCGAAGCATTCGCTGAGTTGAAGGTGAAGGAACTCAAG 5 0.125 No Hit CTTGCTGCTCAGAAGCTGTCTATGAATGTCTGATATTTGTTGGTTGAAGA 5 0.125 No Hit CTGCACTTTAAGAGCTCTCTTTTCCTTTTACCTTTATCTTTCTCCTTTAA 5 0.125 No Hit ATTTTCTAGAAAAAGAGTGTCTTGGGAACTTACAGTAGATTTCATTGGAA 5 0.125 No Hit GAAGAAGAAGAAGAAGAGGAGGAGGATGAACAGGGAGAAAATATAAGCGA 5 0.125 No Hit CAAAGAAGAAAAGATAGAAAATCAAAAACCCCCGAAGCCAAAATATATCC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.1 0.0 0.0 0.0 0.0 56-57 0.1 0.0 0.0 0.0 0.0 58-59 0.1 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.1 0.0 0.0 0.0 0.0 64-65 0.1375 0.0 0.0 0.0 0.0 66-67 0.175 0.0 0.0 0.0 0.0 68-69 0.175 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.1875 0.0 0.0 0.0 0.0 76-77 0.21250000000000002 0.0 0.0 0.0 0.0 78-79 0.2375 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.375 0.0 0.0 0.0 0.0 84-85 0.45 0.0 0.0 0.0 0.0 86-87 0.4625 0.0 0.0 0.0 0.0 88-89 0.625 0.0 0.0 0.0 0.0 90-91 0.8 0.0 0.0 0.0 0.0 92-93 0.8875 0.0 0.0 0.0 0.0 94-95 1.025 0.0 0.0 0.0 0.0 96-97 1.1625 0.0 0.0 0.0 0.0 98-99 1.3625 0.0 0.0 0.0 0.0 100-101 1.7125 0.0 0.0 0.0 0.0 102-103 2.0625 0.0 0.0 0.0 0.0 104-105 2.375 0.0 0.0 0.0 0.0 106-107 2.6125 0.0 0.0 0.0 0.0 108-109 2.8875 0.0 0.0 0.0 0.0 110-111 3.075 0.0 0.0 0.0 0.0 112-113 3.45 0.0 0.0 0.0 0.0 114-115 3.8375 0.0 0.0 0.0 0.0 116-117 4.2125 0.0 0.0 0.0 0.0 118-119 4.625 0.0 0.0 0.0 0.0 120-121 5.0 0.0 0.0 0.0 0.0 122-123 5.4 0.0 0.0 0.0 0.0 124-125 6.0 0.0 0.0 0.0 0.0 126-127 6.4625 0.0 0.0 0.0 0.0 128-129 7.2375 0.0 0.0 0.0 0.0 130-131 7.6875 0.0 0.0 0.0 0.0 132-133 8.25 0.0 0.0 0.0 0.0 134-135 8.9375 0.0 0.0 0.0 0.0 136-137 9.3 0.0 0.0 0.0 0.0 138-139 9.962499999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCCATT 10 0.006830828 145.0 4 CTCAGAG 10 0.006830828 145.0 8 >>END_MODULE Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944031 spots for SRR13695496.sra Written 944031 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra Read 944020 spots for SRR13695496.sra Written 944020 spots for SRR13695496.sra SRR ids: ['SRR13695496.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_cdta8939 SRR13695496.sra spots: 18880411 blocks: [[1, 944020], [944021, 1888040], [1888041, 2832060], [2832061, 3776080], [3776081, 4720100], [4720101, 5664120], [5664121, 6608140], [6608141, 7552160], [7552161, 8496180], [8496181, 9440200], [9440201, 10384220], [10384221, 11328240], [11328241, 12272260], [12272261, 13216280], [13216281, 14160300], [14160301, 15104320], [15104321, 16048340], [16048341, 16992360], [16992361, 17936380], [17936381, 18880411]] SRR13695496 file size 6394689 SRR13695496 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695496 SRR13695496_1.fastq SRR13695496_2.fastq Input file: SRR13695496_1.fastq Paired file: SRR13695496_2.fastq trimmed: SRR13695496-trimmed-pair1.fastq, SRR13695496-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 07:32:24 2025 >> started Wed Feb 12 07:32:45 2025 >> done (21.960s) 18880411 read pairs processed; of these: 119 ( 0.00%) short read pairs filtered out after trimming by size control 5071 ( 0.03%) empty read pairs filtered out after trimming by size control 18875221 (99.97%) read pairs available; of these: 2670962 (14.15%) trimmed read pairs available after processing 16204259 (85.85%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 3 0.00% 21 1 0.00% 22 4 0.00% 23 1 0.00% 24 6 0.00% 25 1 0.00% 26 0 0.00% 27 4 0.00% 28 2 0.00% 29 3 0.00% 30 2 0.00% 31 3 0.00% 32 2 0.00% 33 5 0.00% 34 6 0.00% 35 4 0.00% 36 10 0.00% 37 4 0.00% 38 7 0.00% 39 7 0.00% 40 12 0.00% 41 7 0.00% 42 11 0.00% 43 17 0.00% 44 20 0.00% 45 22 0.00% 46 27 0.00% 47 28 0.00% 48 29 0.00% 49 39 0.00% 50 43 0.00% 51 82 0.00% 52 79 0.00% 53 78 0.00% 54 103 0.00% 55 145 0.00% 56 128 0.00% 57 168 0.00% 58 155 0.00% 59 254 0.00% 60 287 0.00% 61 415 0.00% 62 412 0.00% 63 476 0.00% 64 575 0.00% 65 740 0.00% 66 921 0.00% 67 928 0.00% 68 1094 0.01% 69 1316 0.01% 70 1563 0.01% 71 1589 0.01% 72 2043 0.01% 73 2254 0.01% 74 2762 0.01% 75 3174 0.02% 76 3365 0.02% 77 3619 0.02% 78 4159 0.02% 79 4548 0.02% 80 4864 0.03% 81 5477 0.03% 82 6109 0.03% 83 6772 0.04% 84 7693 0.04% 85 8629 0.05% 86 8928 0.05% 87 9296 0.05% 88 9823 0.05% 89 10425 0.06% 90 11585 0.06% 91 12212 0.06% 92 12689 0.07% 93 13937 0.07% 94 14988 0.08% 95 16077 0.09% 96 16940 0.09% 97 17700 0.09% 98 18420 0.10% 99 19569 0.10% 100 20368 0.11% 101 20885 0.11% 102 21850 0.12% 103 23188 0.12% 104 24334 0.13% 105 25360 0.13% 106 26505 0.14% 107 27254 0.14% 108 28806 0.15% 109 29145 0.15% 110 29625 0.16% 111 31010 0.16% 112 32126 0.17% 113 33238 0.18% 114 34599 0.18% 115 36266 0.19% 116 37451 0.20% 117 38926 0.21% 118 40098 0.21% 119 40307 0.21% 120 42402 0.22% 121 43164 0.23% 122 43975 0.23% 123 45387 0.24% 124 46852 0.25% 125 47268 0.25% 126 49354 0.26% 127 50894 0.27% 128 51584 0.27% 129 51655 0.27% 130 54254 0.29% 131 53647 0.28% 132 54931 0.29% 133 56499 0.30% 134 56673 0.30% 135 58469 0.31% 136 59583 0.32% 137 60483 0.32% 138 61180 0.32% 139 63579 0.34% 140 63566 0.34% 141 64172 0.34% 142 65095 0.34% 143 65647 0.35% 144 67834 0.36% 145 68509 0.36% 146 68599 0.36% 147 69901 0.37% 148 70407 0.37% 149 71595 0.38% 150 72567 0.38% 151 16204259 85.85% 18875221 reads passed initial QC criterion=sequence-density sequence-density=0.51 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=24 prefix-density=0.50 prefix-fanout=2.0 sequence=GTGTTGTCGAATCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=29 fanout-score=61.47 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=7.9 sequence=AAACAGAATATTTACTTTTAGCGCAAGTTTCAATTCATGAGCTCGTTACATCACAAGTTAACATTACAAGCCAATAGTCCCAGCACAGAAAAAACTCTTTTGTTGCTCACTTTCCGGGAACGAAGTTTGTGGCATATGCCCAGGCGTTGTTGTTTACTGGGTCAGAAAGGTGGTCAGCCAGGTTCTCCAGTGGTCCCTTTCCGGTCACAATGGCCTGGACAAAGAATCCGAACATTGAGAACATAGCCAA criterion=sequence-density sequence-density=1.18 sequence-density-rank=1 fanout-score=2.04 fanout-score-rank=26 prefix-density=1.18 prefix-fanout=2.0 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.34 sequence-density-rank=10 fanout-score=7.57 fanout-score-rank=1 prefix-density=0.55 prefix-fanout=4.6 sequence=AAGAAAGCTTACCCTAAC SRR13695496 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 07:33:27 Started mapping on | Feb 12 07:33:27 Finished on | Feb 12 07:35:23 Mapping speed, Million of reads per hour | 585.78 Number of input reads | 18875221 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 17714026 Uniquely mapped reads % | 93.85% Average mapped length | 293.51 Number of splices: Total | 17790513 Number of splices: Annotated (sjdb) | 17433704 Number of splices: GT/AG | 17426488 Number of splices: GC/AG | 301650 Number of splices: AT/AC | 10432 Number of splices: Non-canonical | 51943 Mismatch rate per base, % | 0.36% Deletion rate per base | 0.03% Deletion average length | 2.98 Insertion rate per base | 0.02% Insertion average length | 2.45 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 421978 % of reads mapped to multiple loci | 2.24% Number of reads mapped to too many loci | 87456 % of reads mapped to too many loci | 0.46% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.31% % of reads unmapped: other | 0.14% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 739454 739454 739454 N_multimapping 421978 421978 421978 N_noFeature 514673 17311887 693867 N_ambiguous 329586 1702 105559 UnstrandedReadsAssigned:16869767 PositiveStrandReadsAssigned:400437 NegativeStrandReadsAssigned:16914600 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695496 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695496-trimmed-pair1.fastq SRR13695496-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,875,221 reads, 17,012,373 reads pseudoaligned [quant] estimated average fragment length: 234.434 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,099 rounds 52401 SRR13695496.ke.tsv 34699 SRR13695496.se.tsv 87100 total ==> SRR13695496.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1784.57 590 15.19 Potri.005G024800.1.v4.1 1035 801.566 464 26.596 Potri.004G059700.1.v4.1 961 727.612 1 0.0631448 Potri.007G009000.2.v4.1 1416 1182.57 0 0 Potri.003G141000.2.v4.1 2943 2709.57 1047 17.7535 Potri.016G087400.1.v4.1 270 88.6686 1211 627.497 Potri.015G069301.1.v4.1 564 336.332 0 0 Potri.010G195200.1.v4.1 1773 1539.57 137.901 4.11534 Potri.012G127500.1.v4.1 977 743.595 36 2.22435 ==> SRR13695496.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 149 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 289 Potri.001G212900.v4.1 6 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 6 SRR13695496 completed mapping pipeline successfully