Starting /dee2/code/volunteer_pipeline.sh SRR13857046 current disk space = 3052608688128 free memory = 1578825252 SRR13857046 SRAfilesize 9db4bc560988f0e562d1507f96a4e09a SRR13857046.sra SRR13857046.sra file validated SRR13857046 is paired end SRR13857046 is conventional basespace SRR13857046 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13857046_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.52125 32.0 32.0 32.0 12.0 32.0 2 31.54625 32.0 32.0 32.0 32.0 32.0 3 35.24375 37.0 32.0 37.0 32.0 37.0 4 36.1175 37.0 37.0 37.0 32.0 37.0 5 36.43 37.0 37.0 37.0 37.0 37.0 6 39.99975 41.0 41.0 41.0 37.0 41.0 7 40.087 41.0 41.0 41.0 37.0 41.0 8 40.11975 41.0 41.0 41.0 37.0 41.0 9 40.11475 41.0 41.0 41.0 37.0 41.0 10-14 40.20165 41.0 41.0 41.0 38.6 41.0 15-19 40.10565 41.0 41.0 41.0 37.0 41.0 20-24 40.0831 41.0 41.0 41.0 37.0 41.0 25-29 39.9807 41.0 41.0 41.0 37.0 41.0 30-34 39.928900000000006 41.0 41.0 41.0 37.0 41.0 35-39 39.8497 41.0 41.0 41.0 37.0 41.0 40-44 39.81295 41.0 41.0 41.0 37.0 41.0 45-49 39.76745 41.0 41.0 41.0 37.0 41.0 50-54 39.705200000000005 41.0 41.0 41.0 37.0 41.0 55-59 39.65515 41.0 41.0 41.0 37.0 41.0 60-64 39.56855 41.0 41.0 41.0 37.0 41.0 65-69 39.5184 41.0 41.0 41.0 37.0 41.0 70-74 39.52239999999999 41.0 41.0 41.0 37.0 41.0 75-79 39.2577 41.0 40.2 41.0 36.0 41.0 80-84 39.4188 41.0 41.0 41.0 37.0 41.0 85-89 39.510200000000005 41.0 41.0 41.0 37.0 41.0 90-94 39.42815 41.0 41.0 41.0 37.0 41.0 95-99 39.24225 41.0 41.0 41.0 37.0 41.0 100-104 39.20635 41.0 41.0 41.0 36.0 41.0 105-109 39.237 41.0 41.0 41.0 37.0 41.0 110-114 39.06699999999999 41.0 41.0 41.0 37.0 41.0 115-119 38.8795 41.0 41.0 41.0 34.0 41.0 120-124 38.75255 41.0 41.0 41.0 33.0 41.0 125-129 38.63395 41.0 41.0 41.0 32.0 41.0 130-134 38.2933 41.0 39.4 41.0 32.0 41.0 135-139 38.123400000000004 41.0 38.6 41.0 32.0 41.0 140-144 38.0153 41.0 38.6 41.0 32.0 41.0 145-149 37.6308 41.0 37.0 41.0 27.0 41.0 150 37.6435 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 9.0 24 7.0 25 18.0 26 22.0 27 27.0 28 33.0 29 32.0 30 25.0 31 44.0 32 46.0 33 61.0 34 73.0 35 81.0 36 123.0 37 148.0 38 193.0 39 357.0 40 2699.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.894795127353266 26.993355481727576 15.97452934662237 42.137320044296786 2 19.325 23.05 43.95 13.675 3 17.375 25.45 34.375 22.8 4 19.650000000000002 24.3 32.425 23.625 5 28.675 22.1 27.875 21.349999999999998 6 22.125 25.45 31.15 21.275 7 26.674999999999997 26.200000000000003 27.075 20.05 8 18.375 21.75 36.6 23.275000000000002 9 20.875 21.175 35.975 21.975 10-14 24.154999999999998 25.869999999999997 26.665 23.31 15-19 24.51 24.83 25.695 24.965 20-24 24.435000000000002 25.019999999999996 26.355 24.19 25-29 23.91 24.785 26.045 25.259999999999998 30-34 24.52 25.040000000000003 25.77 24.67 35-39 24.725 24.779999999999998 25.41 25.085 40-44 24.04 25.89 25.215 24.855 45-49 24.404999999999998 25.345000000000002 25.245 25.005 50-54 23.68 25.095 25.47 25.755 55-59 24.195 24.455 25.869999999999997 25.480000000000004 60-64 24.25 25.369999999999997 25.785000000000004 24.595 65-69 24.77 25.89 25.080000000000002 24.26 70-74 24.4 25.1 25.180000000000003 25.319999999999997 75-79 23.965 25.615 25.165 25.255 80-84 24.59 25.8 24.834999999999997 24.775 85-89 24.415 26.450000000000003 24.805 24.33 90-94 24.635 25.77 25.44 24.154999999999998 95-99 24.87 25.735000000000003 24.81 24.585 100-104 25.305122048819527 25.840336134453786 24.964985994397757 23.88955582232893 105-109 23.544999999999998 25.805 25.695 24.955 110-114 24.138276051828505 25.45900245134824 26.094351893541447 24.308369603281804 115-119 24.37 25.72 25.35 24.560000000000002 120-124 24.586063728677903 25.786603971787304 24.896203291481168 24.731129008053625 125-129 24.97 25.424999999999997 25.35 24.255 130-134 24.705 25.71 25.490000000000002 24.095 135-139 24.473355016262197 26.419814861145856 25.253940455341507 23.852889667250437 140-144 24.685000000000002 26.6 24.709999999999997 24.005000000000003 145-149 25.195 27.38 24.08 23.345 150 24.05 26.775 23.974999999999998 25.2 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.0 2 1.5 3 2.0 4 3.5 5 3.0 6 0.5 7 0.5 8 0.5 9 0.0 10 0.0 11 0.5 12 1.5 13 1.5 14 1.0 15 0.5 16 0.5 17 0.5 18 0.5 19 2.0 20 3.0 21 3.5 22 4.5 23 4.5 24 4.5 25 5.5 26 5.5 27 7.5 28 10.5 29 11.0 30 14.0 31 13.5 32 15.5 33 24.0 34 32.0 35 40.5 36 47.5 37 49.5 38 58.5 39 76.5 40 101.5 41 133.0 42 148.0 43 153.0 44 193.0 45 223.0 46 195.5 47 178.0 48 175.5 49 187.5 50 186.5 51 166.5 52 156.5 53 160.0 54 146.5 55 120.5 56 124.5 57 119.5 58 101.5 59 91.5 60 80.0 61 51.5 62 35.5 63 48.0 64 48.5 65 31.0 66 26.5 67 35.0 68 29.5 69 22.0 70 20.0 71 14.0 72 9.5 73 8.0 74 8.0 75 4.5 76 4.0 77 4.5 78 3.5 79 1.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 9.700000000000001 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.04 105-109 0.0 110-114 0.055 115-119 0.0 120-124 0.045 125-129 0.0 130-134 0.0 135-139 0.075 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.575 #Duplication Level Percentage of deduplicated Percentage of total 1 87.81770376862401 75.14999999999999 2 8.910312591294186 15.25 3 2.2494887525562373 5.775 4 0.7595676307332748 2.6 5 0.175284837861525 0.75 6 0.05842827928717499 0.3 7 0.029214139643587496 0.17500000000000002 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTATGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAG 7 0.17500000000000002 No Hit CTTTGTGTTTGACACCTCTAGCTTCAAATTCCGAAGGTCTAAAGGATCGA 6 0.15 No Hit CTATGAAATACGAATGCCCCCGACTGTCCCTGTTAATCATTACTCCGATC 6 0.15 No Hit CTTTGTGTTTGAGCCCCCAACTTTCGTTCTTGATTAATGAAAACATCCTT 5 0.125 No Hit CTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCCAAACT 5 0.125 No Hit CTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACAC 5 0.125 No Hit CGGAAGACATTGTCAGGTGGGGAGTTTGGCTGGGGCGGCACATCTGTTAA 5 0.125 No Hit CTCTCAAGTCATTTCACAAAGTCGGACTAGAGTCAAGCTCAACAGGGTCT 5 0.125 No Hit CGTCTTTCATAAATCCAAGAATTTCACCTCTGACTATGAAATACGAATGC 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.1625 0.0 0.0 0.0 0.0 136-137 1.375 0.0 0.0 0.0 0.0 138 1.975 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGGAT 10 0.005077987 159.88889 1 TCGACTT 10 0.0069881454 143.9 9 TTCGACT 10 0.0069881454 143.9 8 CGGATTT 10 0.0069881454 143.9 3 GCGGATT 10 0.0069881454 143.9 2 TTTGTGT 10 0.0069881454 143.9 2 TTTCGAC 10 0.0069881454 143.9 7 >>END_MODULE SRR13857046 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13857046_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 20.14875 32.0 2.0 32.0 2.0 32.0 2 30.28 32.0 32.0 32.0 27.0 32.0 3 31.5325 32.0 32.0 37.0 22.0 37.0 4 33.10375 37.0 32.0 37.0 27.0 37.0 5 34.18125 37.0 37.0 37.0 27.0 37.0 6 36.89 41.0 37.0 41.0 27.0 41.0 7 37.19675 41.0 37.0 41.0 27.0 41.0 8 37.016 41.0 37.0 41.0 27.0 41.0 9 37.5455 41.0 37.0 41.0 27.0 41.0 10-14 38.411899999999996 41.0 40.2 41.0 32.0 41.0 15-19 38.586149999999996 41.0 40.2 41.0 32.0 41.0 20-24 38.52329999999999 41.0 41.0 41.0 32.0 41.0 25-29 38.464150000000004 41.0 40.2 41.0 32.0 41.0 30-34 38.50895 41.0 39.4 41.0 32.0 41.0 35-39 38.5851 41.0 41.0 41.0 32.0 41.0 40-44 38.551199999999994 41.0 40.2 41.0 32.0 41.0 45-49 38.4157 41.0 40.2 41.0 32.0 41.0 50-54 38.3115 41.0 38.6 41.0 31.0 41.0 55-59 38.362849999999995 41.0 38.6 41.0 32.0 41.0 60-64 38.127700000000004 41.0 37.0 41.0 32.0 41.0 65-69 38.480000000000004 41.0 41.0 41.0 32.0 41.0 70-74 38.363600000000005 41.0 40.2 41.0 32.0 41.0 75-79 37.50605 40.2 36.0 41.0 29.0 41.0 80-84 38.323350000000005 41.0 38.6 41.0 32.0 41.0 85-89 38.1509 41.0 37.0 41.0 31.0 41.0 90-94 37.911649999999995 41.0 37.0 41.0 32.0 41.0 95-99 37.7395 41.0 37.0 41.0 28.0 41.0 100-104 37.44945 41.0 37.0 41.0 27.0 41.0 105-109 37.37385 41.0 37.0 41.0 27.0 41.0 110-114 37.01515 41.0 37.0 41.0 27.0 41.0 115-119 36.712450000000004 41.0 37.0 41.0 26.0 41.0 120-124 36.277 41.0 35.0 41.0 23.0 41.0 125-129 35.97765 41.0 32.0 41.0 22.0 41.0 130-134 35.3698 41.0 32.0 41.0 22.0 41.0 135-139 35.1365 41.0 32.0 41.0 22.0 41.0 140-144 34.50599999999999 40.2 31.0 41.0 18.0 41.0 145-149 33.9731 39.4 30.0 41.0 14.0 41.0 150 33.26325 37.0 27.0 41.0 12.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 9.0 23 26.0 24 37.0 25 48.0 26 52.0 27 68.0 28 77.0 29 69.0 30 97.0 31 95.0 32 119.0 33 98.0 34 128.0 35 166.0 36 199.0 37 273.0 38 336.0 39 700.0 40 1403.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.829043411448328 27.00729927007299 17.479830964271994 40.68382635420669 2 18.875 22.95 43.35 14.825 3 18.9 25.55 34.125 21.425 4 20.724999999999998 23.025000000000002 33.275 22.975 5 29.575000000000003 22.5 28.525 19.400000000000002 6 21.425 25.25 32.375 20.95 7 27.575 24.05 29.349999999999998 19.025 8 18.224999999999998 22.325 37.45 22.0 9 22.0 21.0 35.425000000000004 21.575 10-14 24.36 25.025 27.405 23.21 15-19 24.235 24.635 27.36 23.77 20-24 24.08 25.525 26.314999999999998 24.08 25-29 23.65 25.16 26.540000000000003 24.65 30-34 24.02 24.925 26.41 24.645 35-39 24.32 25.245 26.1 24.335 40-44 24.48 25.81 25.39 24.32 45-49 24.22 25.7 25.885 24.195 50-54 24.745 24.545 26.025 24.685000000000002 55-59 24.495 24.529999999999998 26.07 24.905 60-64 23.93 24.08 27.065 24.925 65-69 24.27 25.275 25.77 24.685000000000002 70-74 24.16 24.490000000000002 27.015 24.335 75-79 24.224999999999998 25.28 25.990000000000002 24.505 80-84 24.25 25.929999999999996 26.25 23.57 85-89 24.6 25.629999999999995 25.535000000000004 24.235 90-94 24.95 25.86 25.195 23.995 95-99 25.085 24.875 25.655 24.385 100-104 25.165 24.52 25.905 24.41 105-109 24.709999999999997 24.32 26.44 24.529999999999998 110-114 24.279999999999998 24.59 26.765 24.365000000000002 115-119 25.115 24.455 26.200000000000003 24.23 120-124 24.6 24.665 25.825 24.91 125-129 25.180000000000003 24.765 25.419999999999998 24.635 130-134 24.42 24.560000000000002 26.445 24.575 135-139 24.08 26.125 25.419999999999998 24.375 140-144 24.77 26.235000000000003 25.185000000000002 23.810000000000002 145-149 25.75 26.534999999999997 24.12 23.595 150 24.675 25.074999999999996 26.450000000000003 23.799999999999997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2.0 1 2.0 2 1.0 3 3.0 4 4.5 5 2.0 6 0.5 7 0.5 8 1.0 9 2.0 10 1.5 11 0.5 12 2.0 13 2.0 14 2.0 15 2.0 16 1.0 17 1.0 18 1.0 19 1.0 20 1.5 21 4.5 22 4.0 23 2.0 24 3.0 25 5.0 26 8.0 27 7.5 28 8.5 29 13.0 30 15.5 31 13.0 32 17.0 33 28.0 34 37.0 35 52.0 36 57.5 37 57.5 38 62.5 39 83.0 40 107.0 41 136.5 42 141.5 43 151.0 44 211.5 45 218.5 46 201.5 47 189.0 48 171.5 49 183.0 50 180.0 51 156.5 52 159.0 53 167.5 54 144.0 55 123.0 56 117.0 57 117.5 58 109.0 59 84.5 60 71.0 61 55.0 62 45.0 63 45.0 64 34.0 65 28.0 66 26.0 67 21.5 68 19.5 69 18.5 70 14.0 71 10.0 72 7.0 73 3.5 74 4.0 75 4.5 76 4.0 77 3.5 78 2.5 79 2.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content fail #Base N-Count 1 34.925 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 91.0 #Duplication Level Percentage of deduplicated Percentage of total 1 91.64835164835165 83.39999999999999 2 7.142857142857142 13.0 3 0.9615384615384616 2.625 4 0.16483516483516483 0.6 5 0.08241758241758242 0.375 6 0.0 0.0 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTGGGTCCAAAAAGAGGGGCAGCGCCCCGCCTCCGATTCACGGAATAAG 5 0.125 No Hit CTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAA 5 0.125 No Hit CTTTTAACAGATGTGCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.1875 0.0 0.0 0.0 0.0 136-137 1.375 0.0 0.0 0.0 0.0 138 2.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTGAC 10 0.007026399 143.6375 2 TTGAGTC 10 0.007026399 143.6375 9 TTTGTGT 35 0.003438706 61.558926 2 GTGTTTG 45 0.009297853 47.87916 3 TGTGTTT 45 0.009297853 47.87916 2 >>END_MODULE Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144547 spots for SRR13857046.sra Written 1144547 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra Read 1144534 spots for SRR13857046.sra Written 1144534 spots for SRR13857046.sra SRR ids: ['SRR13857046.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ao_mdzv0 SRR13857046.sra spots: 22890693 blocks: [[1, 1144534], [1144535, 2289068], [2289069, 3433602], [3433603, 4578136], [4578137, 5722670], [5722671, 6867204], [6867205, 8011738], [8011739, 9156272], [9156273, 10300806], [10300807, 11445340], [11445341, 12589874], [12589875, 13734408], [13734409, 14878942], [14878943, 16023476], [16023477, 17168010], [17168011, 18312544], [18312545, 19457078], [19457079, 20601612], [20601613, 21746146], [21746147, 22890693]] SRR13857046 file size 7712850 SRR13857046 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13857046 SRR13857046_1.fastq SRR13857046_2.fastq Input file: SRR13857046_1.fastq Paired file: SRR13857046_2.fastq trimmed: SRR13857046-trimmed-pair1.fastq, SRR13857046-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 21:50:52 2025 >> started Tue Feb 11 21:51:27 2025 >> done (34.960s) 22890693 read pairs processed; of these: 11 ( 0.00%) short read pairs filtered out after trimming by size control 41 ( 0.00%) empty read pairs filtered out after trimming by size control 22890641 (100.00%) read pairs available; of these: 2858646 (12.49%) trimmed read pairs available after processing 20031995 (87.51%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 2 0.00% 20 2 0.00% 21 10 0.00% 22 7 0.00% 23 1 0.00% 24 6 0.00% 25 11 0.00% 26 9 0.00% 27 13 0.00% 28 13 0.00% 29 17 0.00% 30 5 0.00% 31 18 0.00% 32 17 0.00% 33 17 0.00% 34 19 0.00% 35 14 0.00% 36 23 0.00% 37 16 0.00% 38 14 0.00% 39 23 0.00% 40 19 0.00% 41 31 0.00% 42 15 0.00% 43 24 0.00% 44 20 0.00% 45 25 0.00% 46 26 0.00% 47 24 0.00% 48 35 0.00% 49 31 0.00% 50 28 0.00% 51 30 0.00% 52 37 0.00% 53 33 0.00% 54 43 0.00% 55 65 0.00% 56 41 0.00% 57 36 0.00% 58 43 0.00% 59 74 0.00% 60 33 0.00% 61 98 0.00% 62 62 0.00% 63 98 0.00% 64 39 0.00% 65 98 0.00% 66 102 0.00% 67 146 0.00% 68 119 0.00% 69 90 0.00% 70 117 0.00% 71 80 0.00% 72 253 0.00% 73 80 0.00% 74 76 0.00% 75 91 0.00% 76 54 0.00% 77 82 0.00% 78 64 0.00% 79 74 0.00% 80 55 0.00% 81 85 0.00% 82 79 0.00% 83 87 0.00% 84 67 0.00% 85 87 0.00% 86 63 0.00% 87 100 0.00% 88 112 0.00% 89 88 0.00% 90 62 0.00% 91 124 0.00% 92 86 0.00% 93 66 0.00% 94 40 0.00% 95 60 0.00% 96 88 0.00% 97 66 0.00% 98 58 0.00% 99 68 0.00% 100 54 0.00% 101 75 0.00% 102 57 0.00% 103 63 0.00% 104 61 0.00% 105 52 0.00% 106 54 0.00% 107 66 0.00% 108 61 0.00% 109 62 0.00% 110 65 0.00% 111 92 0.00% 112 76 0.00% 113 79 0.00% 114 63 0.00% 115 65 0.00% 116 60 0.00% 117 71 0.00% 118 99 0.00% 119 88 0.00% 120 112 0.00% 121 133 0.00% 122 146 0.00% 123 147 0.00% 124 162 0.00% 125 149 0.00% 126 178 0.00% 127 158 0.00% 128 167 0.00% 129 206 0.00% 130 195 0.00% 131 195 0.00% 132 186 0.00% 133 1105 0.00% 134 103704 0.45% 135 109256 0.48% 136 112634 0.49% 137 116842 0.51% 138 121701 0.53% 139 124762 0.55% 140 128333 0.56% 141 132632 0.58% 142 136007 0.59% 143 139618 0.61% 144 143049 0.62% 145 148946 0.65% 146 149948 0.66% 147 159584 0.70% 148 200610 0.88% 149 822082 3.59% 150 20031995 87.51% 22890641 reads passed initial QC criterion=sequence-density sequence-density=3.27 sequence-density-rank=1 fanout-score=1.08 fanout-score-rank=44 prefix-density=1.23 prefix-fanout=1.1 sequence=TTTGTGTTTGAG criterion=fanout-score sequence-density=0.04 sequence-density-rank=44 fanout-score=122.21 fanout-score-rank=1 prefix-density=3.31 prefix-fanout=1.4 sequence=CGCATCGCCGGCCCCCATCCACTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCAGACAGCGCCTCGTGGTGCGGCAGGGTCCAGCCACGACGGGGCTCTCACCCTCTCCGGCGCCCCTTTCCAGGGGACTTGGGCCTGGTCCGCCGCTGAGGACGCTTCTCCAGACTACAATTCGGACGCCGCAGGCGCCAGATTCTCAAGCTGGGCATTTCCCGGTTCGCTCGCCGTTACTAGGGGAATCCTTGTAAGTTTCTTTTCCTCCGCTT criterion=sequence-density sequence-density=4.25 sequence-density-rank=1 fanout-score=1.37 fanout-score-rank=49 prefix-density=3.59 prefix-fanout=1.4 sequence=TTTGTGTTTGAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=49 fanout-score=56.09 fanout-score-rank=1 prefix-density=0.40 prefix-fanout=1.0 sequence=TGCTTACCAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGGAAGCTGACTGGCGGGATCCCCTCGAGGGTTGCACCGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAGTGAGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCAGCGATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCGTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGGTGCGAGTTCTATCGGGTAAAGCCAATGATTAGAGGCATCGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAAATAGGTAGGACGGCGCGGCTGCTTCGTTGAGCCGCGCCACGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGG Potential 3prime adapter identified. Now checking if in reference sequence /dee2/code/volunteer_pipeline.sh: line 905: -f: command not found /dee2/code/volunteer_pipeline.sh: line 906: -f: command not found Adapter seq not found in reference. Now shuffling file before clipping skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -t 20 -x TTTGTGTTTGAG -y TTTGTGTTTGAG -o SRR13857046 SRR13857046_1.fastq SRR13857046_2.fastq Input file: SRR13857046_1.fastq Paired file: SRR13857046_2.fastq trimmed: SRR13857046-trimmed-pair1.fastq, SRR13857046-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): TTTGTGTTTGAG -- paired 3' end adapter sequence (-y): TTTGTGTTTGAG -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 21:53:17 2025 >> started Tue Feb 11 21:53:37 2025 >> done (19.626s) 13734385 read pairs processed; of these: 120390 ( 0.88%) short read pairs filtered out after trimming by size control 66359 ( 0.48%) empty read pairs filtered out after trimming by size control 13547636 (98.64%) read pairs available; of these: 2314 ( 0.02%) trimmed read pairs available after processing 13545322 (99.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 1 0.00% 20 1 0.00% 21 8 0.00% 22 5 0.00% 23 1 0.00% 24 1 0.00% 25 5 0.00% 26 2 0.00% 27 6 0.00% 28 10 0.00% 29 10 0.00% 30 4 0.00% 31 13 0.00% 32 10 0.00% 33 7 0.00% 34 14 0.00% 35 9 0.00% 36 13 0.00% 37 8 0.00% 38 14 0.00% 39 8 0.00% 40 14 0.00% 41 19 0.00% 42 10 0.00% 43 12 0.00% 44 13 0.00% 45 11 0.00% 46 15 0.00% 47 14 0.00% 48 19 0.00% 49 21 0.00% 50 13 0.00% 51 19 0.00% 52 21 0.00% 53 23 0.00% 54 28 0.00% 55 37 0.00% 56 24 0.00% 57 25 0.00% 58 25 0.00% 59 46 0.00% 60 16 0.00% 61 59 0.00% 62 31 0.00% 63 56 0.00% 64 20 0.00% 65 61 0.00% 66 62 0.00% 67 92 0.00% 68 67 0.00% 69 55 0.00% 70 70 0.00% 71 43 0.00% 72 151 0.00% 73 42 0.00% 74 48 0.00% 75 52 0.00% 76 29 0.00% 77 47 0.00% 78 41 0.00% 79 36 0.00% 80 32 0.00% 81 51 0.00% 82 47 0.00% 83 54 0.00% 84 37 0.00% 85 49 0.00% 86 39 0.00% 87 55 0.00% 88 73 0.00% 89 57 0.00% 90 39 0.00% 91 73 0.00% 92 52 0.00% 93 35 0.00% 94 28 0.00% 95 40 0.00% 96 55 0.00% 97 36 0.00% 98 38 0.00% 99 40 0.00% 100 30 0.00% 101 39 0.00% 102 39 0.00% 103 41 0.00% 104 37 0.00% 105 37 0.00% 106 38 0.00% 107 45 0.00% 108 34 0.00% 109 36 0.00% 110 44 0.00% 111 62 0.00% 112 50 0.00% 113 51 0.00% 114 36 0.00% 115 38 0.00% 116 38 0.00% 117 43 0.00% 118 55 0.00% 119 57 0.00% 120 64 0.00% 121 92 0.00% 122 91 0.00% 123 81 0.00% 124 95 0.00% 125 74 0.00% 126 106 0.00% 127 86 0.00% 128 110 0.00% 129 129 0.00% 130 118 0.00% 131 118 0.00% 132 125 0.00% 133 666 0.00% 134 61570 0.45% 135 64625 0.48% 136 66512 0.49% 137 69483 0.51% 138 72247 0.53% 139 73886 0.55% 140 75937 0.56% 141 78712 0.58% 142 80920 0.60% 143 82940 0.61% 144 84698 0.63% 145 88282 0.65% 146 89462 0.66% 147 95064 0.70% 148 119296 0.88% 149 485493 3.58% 150 11853135 87.49% criterion=sequence-density sequence-density=2.70 sequence-density-rank=1 fanout-score=1.07 fanout-score-rank=44 prefix-density=1.22 prefix-fanout=1.1 sequence=TTTGTGTTTGAG criterion=fanout-score sequence-density=0.04 sequence-density-rank=44 fanout-score=120.79 fanout-score-rank=1 prefix-density=3.25 prefix-fanout=1.4 sequence=CGCATCGCCGGCCCCCATCCACTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCAGACAGCGCCTCGTGGTGCGGCAGGGTCCAGCCACGACGGGGCTCTCACCCTCTCCGGCGCCCCTTTCCAGGGGACTTGGGCCTGGTCCGCCGCTGAGGACGCTTCTCCAGACTACAATTCGGACGCCGCAGGCGCCAGATTCTCAAGCTGGGCATTTCCCGGTTCGCTCGCCGTTACTAGGGGAATCCTTGTAAGTTTCTTTTCCTCCGCTT criterion=sequence-density sequence-density=3.54 sequence-density-rank=1 fanout-score=1.41 fanout-score-rank=48 prefix-density=3.60 prefix-fanout=1.4 sequence=TTTGTGTTTGAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=48 fanout-score=54.60 fanout-score-rank=1 prefix-density=0.39 prefix-fanout=1.0 sequence=TGCTTACCAAACACGGACCAAGGAGTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGGAAGCTGACTGGCGGGATCCCCTCGAGGGTTGCACCGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAGTGAGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGGCCCGCAGCGATACTGACGTGCAAATCGTTCGTCTGACTTGGGTATAGGGGCGAAAGACTAATCGAACCGTCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGATAGCTGGAGCTCGGTGCGAGTTCTATCGGGTAAAGCCAATGATTAGAGGCATCGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAAATAGGTAGGACGGCGCGGCTGCTTCGTTGAGCCGCGCCACGGAATCGAGAGCTCCAAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGCGG SRR13857046 testing PE reads STAR mapping to Ensembl genome Unpaired reads removal Started job on | Feb 11 22:11:45 Started mapping on | Feb 11 22:11:48 Finished on | Feb 11 22:19:52 Mapping speed, Million of reads per hour | 168.87 Number of input reads | 22703671 Average input read length | 278 UNIQUE READS: Uniquely mapped reads number | 5614176 Uniquely mapped reads % | 24.73% Average mapped length | 269.67 Number of splices: Total | 2649780 Number of splices: Annotated (sjdb) | 2543162 Number of splices: GT/AG | 2568859 Number of splices: GC/AG | 34581 Number of splices: AT/AC | 4131 Number of splices: Non-canonical | 42209 Mismatch rate per base, % | 0.63% Deletion rate per base | 0.01% Deletion average length | 2.27 Insertion rate per base | 0.02% Insertion average length | 2.99 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 541719 % of reads mapped to multiple loci | 2.39% Number of reads mapped to too many loci | 12055400 % of reads mapped to too many loci | 53.10% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 9.80% % of reads unmapped: other | 9.99% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 16547778 16547778 16547778 N_multimapping 541719 541719 541719 N_noFeature 1878951 3753981 3672507 N_ambiguous 119612 26535 26622 UnstrandedReadsAssigned:3615613 PositiveStrandReadsAssigned:1833660 NegativeStrandReadsAssigned:1915047 Dataset is classified unstranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 SRR13857046 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13857046-trimmed-pair1.fastq SRR13857046-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,703,671 reads, 18,339,232 reads pseudoaligned [quant] estimated average fragment length: 183.925 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,004 rounds 52401 SRR13857046.ke.tsv 34699 SRR13857046.se.tsv 87100 total ==> SRR13857046.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1835.07 136 3.00661 Potri.005G024800.1.v4.1 1035 852.075 10 0.476118 Potri.004G059700.1.v4.1 961 778.075 157 8.18598 Potri.007G009000.2.v4.1 1416 1233.07 0 0 Potri.003G141000.2.v4.1 2943 2760.07 96.1915 1.41387 Potri.016G087400.1.v4.1 270 99.9294 204 82.8189 Potri.015G069301.1.v4.1 564 381.279 0 0 Potri.010G195200.1.v4.1 1773 1590.07 0 0 Potri.012G127500.1.v4.1 977 794.075 20 1.02179 ==> SRR13857046.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 285 Potri.001G233950.v4.1 6 Potri.001G122700.v4.1 104 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 62 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 6 SRR13857046 completed mapping pipeline successfully