Starting /dee2/code/volunteer_pipeline.sh SRR15142072
      current disk space = 2796418781184
      free memory = 1575040984 
SRR15142072_1.fastq is conventional basespace
SRR15142072_1.fastq read1 length is 98 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR15142072_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	328948141
Sequences flagged as poor quality	0
Sequence length	98
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.26771193092105	32.0	32.0	32.0	12.0	32.0
2	25.000302901848592	27.0	27.0	27.0	12.0	27.0
3	28.198163527545212	27.0	27.0	37.0	12.0	37.0
4	30.18009454870274	32.0	27.0	37.0	12.0	37.0
5	32.023556272354796	37.0	27.0	37.0	22.0	37.0
6	33.819176819728554	37.0	32.0	41.0	12.0	41.0
7	33.50058125119485	37.0	27.0	41.0	12.0	41.0
8	33.60530927882642	37.0	32.0	41.0	12.0	41.0
9	33.809033986910414	37.0	32.0	41.0	12.0	41.0
10-11	34.570133475841715	37.0	32.0	41.0	22.0	41.0
12-13	34.313857466061805	37.0	32.0	41.0	17.0	41.0
14-15	34.80276573291229	41.0	32.0	41.0	22.0	41.0
16-17	34.30297477650132	37.0	32.0	41.0	12.0	41.0
18-19	34.51997288229089	39.0	32.0	41.0	17.0	41.0
20-21	34.458782521285016	37.0	32.0	41.0	22.0	41.0
22-23	34.31414894665722	37.0	32.0	41.0	12.0	41.0
24-25	34.528543050802654	39.0	32.0	41.0	17.0	41.0
26-27	34.38100646083299	37.0	32.0	41.0	12.0	41.0
28-29	34.23514764444283	37.0	32.0	41.0	12.0	41.0
30-31	34.33005094714915	37.0	32.0	41.0	12.0	41.0
32-33	34.47353987174532	41.0	32.0	41.0	12.0	41.0
34-35	34.51667762427026	41.0	32.0	41.0	12.0	41.0
36-37	34.60811816838934	41.0	32.0	41.0	17.0	41.0
38-39	34.63104364070566	41.0	32.0	41.0	17.0	41.0
40-41	34.66485016098632	41.0	32.0	41.0	12.0	41.0
42-43	34.65846587198071	41.0	32.0	41.0	12.0	41.0
44-45	34.65654159602015	41.0	32.0	41.0	17.0	41.0
46-47	34.63971216362643	41.0	32.0	41.0	12.0	41.0
48-49	34.6926211782422	41.0	32.0	41.0	22.0	41.0
50-51	34.58975928822775	41.0	32.0	41.0	17.0	41.0
52-53	34.64321161188748	41.0	32.0	41.0	22.0	41.0
54-55	34.58161810982844	39.0	32.0	41.0	17.0	41.0
56-57	34.61225089580306	39.0	32.0	41.0	22.0	41.0
58-59	34.60944568463149	39.0	32.0	41.0	22.0	41.0
60-61	34.631773562751334	39.0	32.0	41.0	22.0	41.0
62-63	34.57302254521633	37.0	32.0	41.0	22.0	41.0
64-65	34.6794229443601	41.0	32.0	41.0	22.0	41.0
66-67	34.67846234765619	41.0	32.0	41.0	22.0	41.0
68-69	34.638253713371796	41.0	32.0	41.0	22.0	41.0
70-71	34.60218774119778	39.0	32.0	41.0	17.0	41.0
72-73	34.52598723152535	37.0	32.0	41.0	12.0	41.0
74-75	34.4691590444343	37.0	32.0	41.0	12.0	41.0
76-77	32.67695167032423	34.5	29.5	39.0	12.0	41.0
78-79	33.500279303904016	37.0	29.5	41.0	17.0	41.0
80-81	34.82885847954982	39.0	32.0	41.0	22.0	41.0
82-83	35.10086169023219	41.0	32.0	41.0	22.0	41.0
84-85	35.18486564573715	41.0	32.0	41.0	22.0	41.0
86-87	35.14655555691375	41.0	32.0	41.0	22.0	41.0
88-89	35.327592267803695	41.0	32.0	41.0	22.0	41.0
90-91	35.254973433639194	41.0	32.0	41.0	22.0	41.0
92-93	35.31053122291395	41.0	32.0	41.0	22.0	41.0
94-95	35.23229850689444	41.0	32.0	41.0	22.0	41.0
96-97	35.19856362252553	41.0	32.0	41.0	22.0	41.0
98	35.0601614131025	41.0	32.0	41.0	22.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.24974351598227074
1101	2	0.09621049852854924
1101	3	0.1561073924754055
1101	4	0.22843174182958492
1101	5	0.20440685318843776
1101	6	0.2625899549684263
1101	7	0.4816370420140359
1101	8	0.5470533328283551
1101	9	0.31745596586264924
1101	10-11	0.29564477491651786
1101	12-13	0.29892999965617406
1101	14-15	0.30027978894403873
1101	16-17	0.5573912725296921
1101	18-19	0.49392127379055495
1101	20-21	0.6162832206964381
1101	22-23	0.7841860291176488
1101	24-25	0.6581363374492994
1101	26-27	0.7444717091965103
1101	28-29	0.7399928175978232
1101	30-31	0.8262788189172383
1101	32-33	0.911666866827801
1101	34-35	0.9360485385558519
1101	36-37	1.015939328806624
1101	38-39	0.8224340494852314
1101	40-41	0.9206821506073695
1101	42-43	1.0781879143915347
1101	44-45	1.050258372540938
1101	46-47	1.0175498407173293
1101	48-49	1.0156856239662133
1101	50-51	1.1010760652751586
1101	52-53	1.0710330258929446
1101	54-55	1.1132885915057216
1101	56-57	1.0540590265100036
1101	58-59	0.9037696478548511
1101	60-61	0.9424540274190534
1101	62-63	0.9330245585859132
1101	64-65	1.0928159345847206
1101	66-67	1.0638042764650777
1101	68-69	1.1335828661811433
1101	70-71	1.0134907394325339
1101	72-73	1.0311683829040277
1101	74-75	1.1715933277965647
1101	76-77	1.0186835887238246
1101	78-79	0.9118437023378121
1101	80-81	0.9122880271667881
1101	82-83	0.9038033337925171
1101	84-85	0.9498255469659895
1101	86-87	0.8949357435085901
1101	88-89	0.8862036422036468
1101	90-91	0.9039445416366263
1101	92-93	0.8660853771823795
1101	94-95	1.0304318833714206
1101	96-97	1.1013806783073292
1101	98	0.9370241868204232
1102	1	0.09013650909940196
1102	2	0.05011977655943056
1102	3	0.3559422053275192
1102	4	0.3647324391599156
1102	5	0.22342897955930852
1102	6	0.4100305577633989
1102	7	0.6122157851420766
1102	8	0.6235707146208682
1102	9	0.5195922006232081
1102	10-11	0.45608659366778426
1102	12-13	0.43227832093722185
1102	14-15	0.4422343891075329
1102	16-17	0.6414999791216474
1102	18-19	0.4700468823486972
1102	20-21	0.5747575315720326
1102	22-23	0.6666677737239155
1102	24-25	0.6567289794201798
1102	26-27	0.7677306948476925
1102	28-29	0.7081776388978369
1102	30-31	0.6684686965484943
1102	32-33	0.7084294990413227
1102	34-35	0.7418535624672771
1102	36-37	0.8775931704662412
1102	38-39	0.7998274779084582
1102	40-41	0.7634817350966117
1102	42-43	0.856357583393617
1102	44-45	0.8432656622431907
1102	46-47	0.975316208032126
1102	48-49	0.9358283794005047
1102	50-51	1.1047478391851087
1102	52-53	0.9562495953786012
1102	54-55	1.1177596786413915
1102	56-57	0.9440136979219815
1102	58-59	1.0323285133848472
1102	60-61	0.9176140863275251
1102	62-63	1.08513697148787
1102	64-65	1.1339730206913146
1102	66-67	1.1071543984153536
1102	68-69	1.1936578989358537
1102	70-71	1.2481670082434206
1102	72-73	1.1361720091446799
1102	74-75	1.248618918372273
1102	76-77	1.2251692935414198
1102	78-79	1.183697027843614
1102	80-81	1.0998485446487507
1102	82-83	1.073149306239408
1102	84-85	1.0886634726893263
1102	86-87	1.0948656094817295
1102	88-89	1.0863436878393884
1102	90-91	1.1669484217920072
1102	92-93	1.1306351016530414
1102	94-95	1.2133440471789356
1102	96-97	1.2953696746587795
1102	98	1.1220800570703986
1103	1	0.43472886479227313
1103	2	0.29826875255229623
1103	3	0.7598380057838128
1103	4	0.6535089030340551
1103	5	0.6732377697498961
1103	6	0.7261588036202156
1103	7	0.9223619220003201
1103	8	1.035022135829955
1103	9	0.9693705983699132
1103	10-11	0.8798450336253154
1103	12-13	0.9201601102467976
1103	14-15	0.96423976460364
1103	16-17	1.2301075733037052
1103	18-19	1.1046589297438771
1103	20-21	1.0635529985791834
1103	22-23	1.2173555385928978
1103	24-25	1.209681476355989
1103	26-27	1.227063451334864
1103	28-29	1.224239048982362
1103	30-31	1.1771878987753936
1103	32-33	1.2818684515205234
1103	34-35	1.188450873606385
1103	36-37	1.2621851841803817
1103	38-39	1.224630660675146
1103	40-41	1.2590680299437977
1103	42-43	1.3019670225735354
1103	44-45	1.326384532890664
1103	46-47	1.3235269073314626
1103	48-49	1.2970215289506015
1103	50-51	1.3992301877771567
1103	52-53	1.4098123917684404
1103	54-55	1.4459544927102854
1103	56-57	1.41344676926024
1103	58-59	1.2835363867491338
1103	60-61	1.3519440089620147
1103	62-63	1.4794510612906606
1103	64-65	1.5561105129727792
1103	66-67	1.5054151980431172
1103	68-69	1.626869066319074
1103	70-71	1.598540175726363
1103	72-73	1.5148091170516622
1103	74-75	1.6304045711596373
1103	76-77	1.6229041454723472
1103	78-79	1.5342439112714104
1103	80-81	1.5271653873453843
1103	82-83	1.5628914258134827
1103	84-85	1.6128873512116328
1103	86-87	1.600641885377975
1103	88-89	1.594368496028764
1103	90-91	1.7009413091810188
1103	92-93	1.6013433309410487
1103	94-95	1.702669917269354
1103	96-97	1.8548562242475484
1103	98	1.7354490575002117
1104	1	0.18128109801111947
1104	2	0.031630391789981616
1104	3	0.22933754903624148
1104	4	0.7675694589499251
1104	5	0.2328076135191708
1104	6	0.31946030883755583
1104	7	0.5223244560096134
1104	8	0.49992016513709814
1104	9	0.45465600193248434
1104	10-11	0.3043374441779676
1104	12-13	0.3544706967267217
1104	14-15	0.2925195960493241
1104	16-17	0.46738749824357484
1104	18-19	0.3974649008134037
1104	20-21	0.3813127080878189
1104	22-23	0.497988715648475
1104	24-25	0.45473160658232814
1104	26-27	0.49621260876337203
1104	28-29	0.5110862847344109
1104	30-31	0.48071873003459586
1104	32-33	0.5520531145953242
1104	34-35	0.5569834939014129
1104	36-37	0.50100980557594
1104	38-39	0.469944233607805
1104	40-41	0.4981752361506224
1104	42-43	0.5879688044759916
1104	44-45	0.5838360653595842
1104	46-47	0.5381380622427869
1104	48-49	0.5421673661476802
1104	50-51	0.5961172139957824
1104	52-53	0.652933067333386
1104	54-55	0.7228266155409955
1104	56-57	0.6116868586916056
1104	58-59	0.5351333137007899
1104	60-61	0.5522889429084188
1104	62-63	0.6326741130882212
1104	64-65	0.7337982294338019
1104	66-67	0.6451218588476948
1104	68-69	0.6929913332511433
1104	70-71	0.6962288590140489
1104	72-73	0.6635084060643734
1104	74-75	0.8559754463826437
1104	76-77	0.6590792953292137
1104	78-79	0.620760671662957
1104	80-81	0.5233271027919599
1104	82-83	0.586897086879425
1104	84-85	0.7008662808901036
1104	86-87	0.6948797471700274
1104	88-89	0.6172156099814217
1104	90-91	0.6537340388542887
1104	92-93	0.6091046848972184
1104	94-95	0.7769995193760124
1104	96-97	0.8476936719092052
1104	98	0.7693430723650181
1105	1	0.20211578522646434
1105	2	-0.08921503420051735
1105	3	-0.06254306543102928
1105	4	0.09867329900053434
1105	5	0.16009312873209325
1105	6	0.34366850592765985
1105	7	0.30983394646275997
1105	8	0.41261510112119026
1105	9	0.3960620202210947
1105	10-11	0.2980871472000146
1105	12-13	0.23270376069746845
1105	14-15	0.2252560074888308
1105	16-17	0.31558988777553765
1105	18-19	0.2939798794506032
1105	20-21	0.21253786962682142
1105	22-23	0.32881550867003284
1105	24-25	0.3181519056289801
1105	26-27	0.4534479815333583
1105	28-29	0.3521158386003975
1105	30-31	0.3536403634344154
1105	32-33	0.2612447109495548
1105	34-35	0.3856903808456522
1105	36-37	0.3086581370339161
1105	38-39	0.39833495563748045
1105	40-41	0.39062385738056804
1105	42-43	0.5194078045670096
1105	44-45	0.4748571724120225
1105	46-47	0.4617672401872355
1105	48-49	0.4198772904763146
1105	50-51	0.3977946403821022
1105	52-53	0.39314133957346087
1105	54-55	0.4724524526591267
1105	56-57	0.3964557309609589
1105	58-59	0.33529372329033436
1105	60-61	0.384080577121793
1105	62-63	0.40526068500822277
1105	64-65	0.5200014963351691
1105	66-67	0.38296855105139826
1105	68-69	0.5287508243340255
1105	70-71	0.4568145245447326
1105	72-73	0.3037120769712871
1105	74-75	0.5533824464938419
1105	76-77	0.5792367817632069
1105	78-79	0.44088205500650446
1105	80-81	0.3405853900606175
1105	82-83	0.3943575255029259
1105	84-85	0.43202855419939823
1105	86-87	0.4806265118034645
1105	88-89	0.43398063290877076
1105	90-91	0.4405470674819014
1105	92-93	0.3438532303499855
1105	94-95	0.5137273764702499
1105	96-97	0.6899311956277927
1105	98	0.2644033418651901
1106	1	-0.14919508184746277
1106	2	0.07071534980863348
1106	3	0.26173636016669377
1106	4	0.16456154906444453
1106	5	0.239155656797017
1106	6	0.17511324693307984
1106	7	0.3808964556663028
1106	8	0.3633345815359377
1106	9	0.3798986234377324
1106	10-11	0.31837992366642
1106	12-13	0.32700070741075393
1106	14-15	0.4174309339803486
1106	16-17	0.5051564370018227
1106	18-19	0.408332528361953
1106	20-21	0.403896700303747
1106	22-23	0.45152543536198664
1106	24-25	0.4967907966665095
1106	26-27	0.5197753386556698
1106	28-29	0.5647297452612534
1106	30-31	0.4769042358866997
1106	32-33	0.5434861368895838
1106	34-35	0.45996486849475104
1106	36-37	0.5822913921255832
1106	38-39	0.5173239516893489
1106	40-41	0.5338747539720927
1106	42-43	0.5783810640033309
1106	44-45	0.6793516530487338
1106	46-47	0.7092412690466645
1106	48-49	0.6983433972477471
1106	50-51	0.6356777929025412
1106	52-53	0.7125011992386732
1106	54-55	0.7232069835684882
1106	56-57	0.622925621083688
1106	58-59	0.5890731645572771
1106	60-61	0.49558034417253793
1106	62-63	0.6216899611767417
1106	64-65	0.7766105549312812
1106	66-67	0.7340398727983839
1106	68-69	0.8217789841778114
1106	70-71	0.7246841567657114
1106	72-73	0.6624728650322282
1106	74-75	0.8642735019907803
1106	76-77	0.4942771188887818
1106	78-79	0.5909110892074239
1106	80-81	0.6410022813068466
1106	82-83	0.5681796935240371
1106	84-85	0.6261157823780366
1106	86-87	0.664089517620063
1106	88-89	0.6005758399885082
1106	90-91	0.6083601468678026
1106	92-93	0.573880028157312
1106	94-95	0.6996479570781631
1106	96-97	0.7477971126370448
1106	98	0.6112836910416561
1107	1	0.44560220845503906
1107	2	0.3720395339977358
1107	3	0.8249229764888497
1107	4	0.8049964337099205
1107	5	0.7061183107437259
1107	6	0.9055208513067825
1107	7	0.929710442919756
1107	8	1.087057849760896
1107	9	1.0244702135706234
1107	10-11	1.034336907899636
1107	12-13	0.9867743671073583
1107	14-15	1.023207610583917
1107	16-17	1.21654031346484
1107	18-19	1.0588382462118702
1107	20-21	1.1539099097748888
1107	22-23	1.2535131221505367
1107	24-25	1.1831164949119568
1107	26-27	1.2746145951265007
1107	28-29	1.154230214688397
1107	30-31	1.1686549677187585
1107	32-33	1.251760384543985
1107	34-35	1.2817633048676385
1107	36-37	1.2887871074690338
1107	38-39	1.0736078115558314
1107	40-41	1.185428235807997
1107	42-43	1.2604300476637036
1107	44-45	1.308946264335475
1107	46-47	1.3053581088042492
1107	48-49	1.2984096284839808
1107	50-51	1.337047298206798
1107	52-53	1.3629483016517696
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2224	18-19	-0.3924414027980987
2224	20-21	-0.49879608415228205
2224	22-23	-0.5473324595701712
2224	24-25	-0.3944868935570014
2224	26-27	-0.35571261494584405
2224	28-29	-0.6002042314327269
2224	30-31	-0.5702678646223589
2224	32-33	-0.5687568514435668
2224	34-35	-0.5239483435657206
2224	36-37	-0.639174767216609
2224	38-39	-0.572934896275811
2224	40-41	-0.5692149942530804
2224	42-43	-0.5427767704058652
2224	44-45	-0.4864318715099145
2224	46-47	-0.45396076889444004
2224	48-49	-0.3916941095982409
2224	50-51	-0.357001075598518
2224	52-53	-0.43395885784703836
2224	54-55	-0.38644845274274076
2224	56-57	-0.3099253864562854
2224	58-59	-0.3377725003506882
2224	60-61	-0.47940018712513677
2224	62-63	-0.42588191358883165
2224	64-65	-0.4932944257542715
2224	66-67	-0.3076491653097406
2224	68-69	-0.5321207501258627
2224	70-71	-0.9390229549970215
2224	72-73	-0.5940504056484954
2224	74-75	-0.499650005401854
2224	76-77	-0.1661155890289976
2224	78-79	-0.2858340115508966
2224	80-81	-0.3074625265901645
2224	82-83	-0.30038234637037675
2224	84-85	-0.352980554926269
2224	86-87	-0.39576410367764225
2224	88-89	-0.3351461338629349
2224	90-91	-0.38526822073822586
2224	92-93	-0.22116821054500946
2224	94-95	-0.5478541461089037
2224	96-97	-0.42068492926247103
2224	98	-0.364406992870002
2225	1	0.0921513509885159
2225	2	-0.11433415916657452
2225	3	0.03778732247997496
2225	4	0.016323700461523316
2225	5	-0.09524525184351162
2225	6	-0.0124726836289355
2225	7	-0.12660616560827975
2225	8	-0.23266430513815806
2225	9	-0.22948882308573815
2225	10-11	-0.24536796724444798
2225	12-13	-0.16985505987982208
2225	14-15	-0.13757321232551334
2225	16-17	-0.31439100307215284
2225	18-19	-0.2366426714980463
2225	20-21	-0.17181121446945014
2225	22-23	-0.1412333350425854
2225	24-25	-0.16713354360671673
2225	26-27	-0.17851065324388316
2225	28-29	-0.3014216569971211
2225	30-31	-0.2999569668301092
2225	32-33	-0.3774173626296431
2225	34-35	-0.15828949280462723
2225	36-37	-0.18400500352831983
2225	38-39	-0.06184394121797254
2225	40-41	-0.06217641925526607
2225	42-43	-0.1566852861416521
2225	44-45	-0.13298857656346286
2225	46-47	-0.023811855377978475
2225	48-49	-0.07813904974271679
2225	50-51	-0.030609196806835826
2225	52-53	-0.05731983195507695
2225	54-55	-0.003574177659558586
2225	56-57	0.03535999989552607
2225	58-59	-0.0073868625058821635
2225	60-61	-0.069013544081443
2225	62-63	-0.10782713760693241
2225	64-65	-0.15370157503019044
2225	66-67	0.006046820848801815
2225	68-69	-0.05856737638266907
2225	70-71	-0.05418315515161254
2225	72-73	0.028978460820702878
2225	74-75	0.0612461430390141
2225	76-77	0.07680638867422829
2225	78-79	0.029357005265829628
2225	80-81	0.18042887901710003
2225	82-83	0.08461612963279208
2225	84-85	0.045891772743246406
2225	86-87	-0.07652185190888616
2225	88-89	-0.023294789359397328
2225	90-91	0.04783732898595616
2225	92-93	0.16011421359799982
2225	94-95	-0.017282370708677774
2225	96-97	-0.09499056512461124
2225	98	0.05716956959646069
2226	1	-0.08784655981787992
2226	2	-0.16288854531911312
2226	3	-0.18405218731000517
2226	4	-0.2563591713433979
2226	5	-0.4123420531296027
2226	6	-0.47621108969746473
2226	7	-0.5707302146112525
2226	8	-0.6422526637335153
2226	9	-0.5046384807397359
2226	10-11	-0.5795075968281935
2226	12-13	-0.627846732722368
2226	14-15	-0.5561915692216743
2226	16-17	-0.6967875224432589
2226	18-19	-0.6845941612072295
2226	20-21	-0.5468916830195596
2226	22-23	-0.562193510060716
2226	24-25	-0.5738239793500455
2226	26-27	-0.5338740649451594
2226	28-29	-0.5557544847813318
2226	30-31	-0.6822345548720818
2226	32-33	-0.7532396147951275
2226	34-35	-0.6135135197473289
2226	36-37	-0.7233867727119474
2226	38-39	-0.6596184557812848
2226	40-41	-0.7739977182288058
2226	42-43	-0.7815636526453176
2226	44-45	-0.6519439230909185
2226	46-47	-0.5436444660854107
2226	48-49	-0.5216857077438632
2226	50-51	-0.6448592710738552
2226	52-53	-0.6460401175428387
2226	54-55	-0.5516982816120048
2226	56-57	-0.4470701929286136
2226	58-59	-0.5446393529976277
2226	60-61	-0.6478929134028562
2226	62-63	-0.7150700725677197
2226	64-65	-0.7342895182803773
2226	66-67	-0.5769009327723396
2226	68-69	-0.6432525701641794
2226	70-71	-0.7526054565710965
2226	72-73	-0.6861763575624948
2226	74-75	-0.7678972289779367
2226	76-77	-0.7098312873579502
2226	78-79	-0.6975309167043093
2226	80-81	-0.6754444558721175
2226	82-83	-0.8035192285737267
2226	84-85	-0.8033225964446089
2226	86-87	-0.6902241165082188
2226	88-89	-0.7059985844923347
2226	90-91	-0.7437768953889403
2226	92-93	-0.6862756399895105
2226	94-95	-0.6879950970926032
2226	96-97	-0.8646519659045211
2226	98	-0.8371224461474824
2227	1	0.10581228673157383
2227	2	0.0560304728970209
2227	3	0.3037970302279547
2227	4	0.14577330645497355
2227	5	0.14158856850819745
2227	6	0.15698993854354626
2227	7	-0.06794337688043584
2227	8	0.020249722147021032
2227	9	-0.029452542686243532
2227	10-11	0.17404767709331992
2227	12-13	0.09479380362729017
2227	14-15	0.24212712488357369
2227	16-17	-0.015960126534835695
2227	18-19	-0.0011510043119358215
2227	20-21	0.163288493549949
2227	22-23	0.20644100076204097
2227	24-25	0.2391984379481613
2227	26-27	0.2596460952061719
2227	28-29	0.22855637247867833
2227	30-31	0.11806664889979146
2227	32-33	0.22589876248576957
2227	34-35	0.38309884782512427
2227	36-37	0.23912018059422024
2227	38-39	0.3291587292286664
2227	40-41	0.3160445352485439
2227	42-43	0.31649434463295734
2227	44-45	0.43405341077527737
2227	46-47	0.5813392869095182
2227	48-49	0.5422787247100516
2227	50-51	0.6305892458599516
2227	52-53	0.5596558493540442
2227	54-55	0.5252573893568382
2227	56-57	0.6692757359507553
2227	58-59	0.5374850999168146
2227	60-61	0.5139538616687815
2227	62-63	0.487624163814921
2227	64-65	0.44567797346938676
2227	66-67	0.6025699076733204
2227	68-69	0.506507298731087
2227	70-71	0.5541842234337722
2227	72-73	0.7467205838830466
2227	74-75	0.8076743387097736
2227	76-77	0.846879054872737
2227	78-79	0.7442370224201014
2227	80-81	0.8281956622690458
2227	82-83	0.7510286017101535
2227	84-85	0.7283542956400737
2227	86-87	0.6763061041796803
2227	88-89	0.6875165805532646
2227	90-91	0.6427052769396866
2227	92-93	0.7143013747364577
2227	94-95	0.628910800045567
2227	96-97	0.5667028866024495
2227	98	0.5971248375606848
2228	1	0.021647722815018255
2228	2	-0.10426834666791507
2228	3	-0.08319477100707573
2228	4	-0.16327742053794836
2228	5	-0.055128199812230605
2228	6	-0.243684557708697
2228	7	-0.3932700215273073
2228	8	-0.5365441236522486
2228	9	-0.5451894154033639
2228	10-11	-0.5198816822776422
2228	12-13	-0.4797368911243467
2228	14-15	-0.5290328821188055
2228	16-17	-0.6992067004725513
2228	18-19	-0.5784815148306137
2228	20-21	-0.5346541324420073
2228	22-23	-0.44446645831777687
2228	24-25	-0.5265684911756523
2228	26-27	-0.43830234959184367
2228	28-29	-0.5337362510913124
2228	30-31	-0.5549658305474239
2228	32-33	-0.5625308849325705
2228	34-35	-0.4678966449973814
2228	36-37	-0.5095559896934958
2228	38-39	-0.5600637713798875
2228	40-41	-0.5955035774988673
2228	42-43	-0.5310320683663576
2228	44-45	-0.5671642704952404
2228	46-47	-4.188580855711269
2228	48-49	-1.370454700303675
2228	50-51	-0.5838236507319152
2228	52-53	-0.3753086016349272
2228	54-55	-0.409790183758318
2228	56-57	-0.2822111007587793
2228	58-59	-0.24355366367000642
2228	60-61	-0.3767413229031007
2228	62-63	-0.4361311691068934
2228	64-65	-0.47930783926017284
2228	66-67	-0.3460960646293927
2228	68-69	-0.5063914343866145
2228	70-71	-0.4739354885506728
2228	72-73	-0.3150112953612805
2228	74-75	-0.3169744186038912
2228	76-77	-0.22948461671466447
2228	78-79	-0.3785458946232083
2228	80-81	-0.2468227342426772
2228	82-83	-0.2568289184982646
2228	84-85	-0.4040379786348538
2228	86-87	-0.43339482081173486
2228	88-89	-0.45197433795409125
2228	90-91	-0.3870417977404017
2228	92-93	-0.2596482377745488
2228	94-95	-0.34320464844904564
2228	96-97	-0.4832127765247449
2228	98	-0.40732852065493574
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2021.0
13	36483.0
14	247747.0
15	820302.0
16	1599274.0
17	2423977.0
18	2992978.0
19	3245292.0
20	3444092.0
21	3681882.0
22	3961450.0
23	4295040.0
24	4685040.0
25	5155635.0
26	5704721.0
27	6385529.0
28	7209351.0
29	8209779.0
30	9384628.0
31	1.0802152E7
32	1.2608341E7
33	1.496896E7
34	1.8188932E7
35	2.2787395E7
36	2.9723928E7
37	4.0203254E7
38	5.2313359E7
39	4.6525313E7
40	7341286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.105916019721214	30.316350281862697	10.826771775527568	18.750961922888525
2	21.17233056966069	30.866082365377473	31.726159536104227	16.235427528857613
3	24.348979497289484	28.274187225116986	30.43587080746685	16.94096247012668
4	23.949344449466153	28.08092893950569	25.69262514108391	22.277101469944252
5	22.63453983161437	34.05228211944812	26.975752387668912	16.337425661268597
6	28.887573322170688	29.715134857076524	25.93124639460833	15.46604542614445
7	21.938607803435946	25.35636218458009	38.13293749305102	14.572092518932944
8	26.172421739882296	24.156782672836023	31.364932576917226	18.305863010364455
9	26.367960535153166	25.554021294803427	30.657540636473758	17.420477533569645
10-11	22.327996375574592	30.85652823312353	29.418555066404828	17.396920324897046
12-13	22.267210656803268	26.70035206428875	30.090587209020313	20.941850069887668
14-15	22.239673335013617	32.335964622460054	27.690137485835496	17.734224556690837
16-17	24.986611500245708	27.331732059323304	27.369098453499056	20.312557986931928
18-19	22.745150883828828	30.522205778326622	27.492492654032052	19.240150683812498
20-21	25.105030723788303	29.78837128960754	28.299408533697388	16.807189452906762
22-23	24.311439251417333	27.084328639497794	31.277210254954156	17.327021854130713
24-25	21.830009417338868	29.716939479766673	29.002248290834036	19.450802812060424
26-27	23.029563179156494	28.99809325954031	31.609525430428686	16.36281813087451
28-29	28.44836984786305	26.532094731933785	28.60314536947271	16.416390050730456
30-31	25.78505777578583	27.87310317734039	29.49617179528946	16.845667251584317
32-33	23.16923706924812	28.66931686791671	30.725225390768678	17.436220672066487
34-35	23.07465859793383	28.477335124991633	30.804975426202514	17.643030850872023
36-37	22.81905897781116	28.606334090814457	31.15673955547424	17.417867375900144
38-39	22.732663049158255	28.58297107689081	31.20883558968038	17.47553028427055
40-41	22.833358860504763	28.50837746590253	31.29703047568828	17.361233197904426
42-43	22.701296575590174	28.315038421930623	31.612895422818788	17.370769579660415
44-45	22.567857740226597	28.30833234591832	31.67552875758614	17.448281156268944
46-47	22.621013912012643	28.33534226042456	31.665636109605554	17.37800771795724
48-49	22.544719622687275	28.486066284892352	31.714602077140285	17.254612015280085
50-51	22.394254078961364	28.444855410540825	31.814607026043113	17.346283484454695
52-53	22.45643240313298	28.34805710388812	31.96612022630394	17.229390266674965
54-55	22.498756877389823	28.284826049695116	32.033772423891364	17.182644649023704
56-57	22.267349861479076	28.32793329715547	32.24002455063176	17.1646922907337
58-59	22.316748084693298	28.402719044948675	32.177677269290584	17.10285560106744
60-61	22.253601831396047	28.356260543626004	32.33710551003066	17.053032114947285
62-63	22.124960300201614	28.393128148821173	32.39029348775926	17.09161806321796
64-65	22.16283883291331	28.34141137667418	32.45884555272406	17.036904237688457
66-67	22.126930734709767	28.36598404983969	32.559602011235974	16.94748320421457
68-69	21.905437230149538	28.410166591030432	32.74163701633858	16.94275916248144
70-71	22.0639091558204	28.37659173760158	32.61635790791716	16.943141198660854
72-73	21.876171043680397	28.38032525625173	32.883060369234016	16.86044333083385
74-75	21.786649524906274	28.502944633177112	32.91268398724766	16.79772185466895
76-77	21.891794071812193	28.41068771004423	32.98778649024518	16.7097317278984
78-79	21.69085523818254	28.48149587495411	33.17406919331807	16.653579693545282
80-81	21.64593552149	28.529522530422202	33.23260930056449	16.591932647523308
82-83	21.671096022397037	28.521400125498808	33.19593984268785	16.611564009416306
84-85	21.63365829774316	28.577872996504805	33.31597170624074	16.47249699951129
86-87	21.35897840146404	28.77727638254697	33.35647999505137	16.507265220937615
88-89	21.410385246519294	28.7920190762594	33.3461810526287	16.45141462459261
90-91	21.26468675027613	28.937765019443656	33.45927852010688	16.338269710173332
92-93	21.210811714626818	29.058471142160812	33.42124515624224	16.30947198697013
94-95	21.24596010414247	29.11572900573816	33.423354394707125	16.21495649541224
96-97	21.081044708230536	29.201360221677675	33.5166956987735	16.200899371318283
98	20.99790213901685	29.396741542298706	33.55890648813852	16.04644983054592
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104992.0
1	168661.5
2	262033.5
3	266016.0
4	222385.0
5	202246.5
6	206785.0
7	217712.0
8	226205.0
9	237437.5
10	250159.5
11	266528.0
12	293571.5
13	330313.5
14	380069.0
15	577727.5
16	683089.5
17	642369.0
18	699315.0
19	791896.0
20	912436.0
21	1050936.0
22	1223813.0
23	1435371.0
24	1743978.0
25	2231681.5
26	2790080.5
27	3408936.0
28	4206364.5
29	5269103.0
30	6561518.5
31	7890458.5
32	9235258.0
33	1.07080115E7
34	1.22538925E7
35	1.3828309E7
36	1.50255435E7
37	1.5665114E7
38	1.630516E7
39	1.67757365E7
40	1.70143465E7
41	1.6999889E7
42	1.65636245E7
43	1.59402745E7
44	1.515938E7
45	1.4047809E7
46	1.2693838E7
47	1.13091525E7
48	9822409.5
49	8449457.5
50	7846790.0
51	7039644.0
52	5566108.0
53	4380307.5
54	3441865.0
55	2749759.0
56	2235859.5
57	1764059.5
58	1347786.0
59	1031591.0
60	877205.0
61	775482.0
62	667650.5
63	600348.5
64	504165.0
65	418665.5
66	399120.5
67	387791.5
68	316486.0
69	238986.0
70	179902.5
71	122309.5
72	85792.5
73	66423.0
74	55720.5
75	42025.0
76	29220.0
77	21330.0
78	14850.5
79	11622.0
80	8676.5
81	6312.0
82	5323.0
83	4690.0
84	4164.0
85	3663.0
86	3317.0
87	3066.5
88	2787.5
89	2573.0
90	2425.5
91	2332.0
92	2264.0
93	2158.0
94	2067.0
95	1987.5
96	2028.5
97	2312.0
98	3278.5
99	6880.5
100	9721.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9039318449895115
2	0.017597302670271056
3	0.0036698793807744914
4	0.0022395627400733664
5	0.0
6	0.0010022856459918404
7	0.0018367636861033364
8	3.5051117677542976E-4
9	0.0
10-11	0.0
12-13	8.055981079400598E-5
14-15	0.0
16-17	0.0024886901549627544
18-19	0.0
20-21	0.0015885482690719932
22-23	0.001574260302629283
24-25	0.001972803366595101
26-27	5.946226034455686E-4
28-29	2.3590344594773074E-4
30-31	0.001077829468566597
32-33	4.7119889332343116E-4
34-35	0.0
36-37	6.718384220934083E-4
38-39	0.0
40-41	3.8410309788010024E-4
42-43	1.5944762551492881E-4
44-45	0.0
46-47	9.329738087803937E-4
48-49	3.857750939531833E-4
50-51	6.581584542227282E-5
52-53	0.0016920600259601407
54-55	3.9656706860672E-4
56-57	5.791186398587976E-5
58-59	0.0011830132215278272
60-61	0.0070257274990953664
62-63	0.00133911685489659
64-65	5.66958668418193E-5
66-67	2.0170352627103008E-4
68-69	5.926466080864703E-4
70-71	0.0
72-73	3.678391360782914E-5
74-75	6.29278522051292E-5
76-77	9.530377616573915E-5
78-79	4.985588290647917E-5
80-81	0.0
82-83	0.0
84-85	1.451596590722183E-4
86-87	1.763195858887678E-5
88-89	1.063997501052909E-6
90-91	0.0012512610612382211
92-93	1.63855615162148E-4
94-95	9.271978223461065E-5
96-97	0.0015627083297607084
98	0.0010825414574998312
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3.28948141E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.267437356681302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.37095392143046	17.769563862368777
2	9.322033261202108	4.3379964988383914
3	3.8812856499319106	2.7092271216953043
4	2.124275336304593	1.977057732632409
5	1.405883529157464	1.6355653472730662
6	0.9939581743159599	1.3876115733634746
7	0.7069311686847456	1.1513933678000474
8	0.5150729253626558	0.9587541619998542
9	0.40578790093621536	0.8497480108619331
>10	3.3282267301811572	15.728628772511843
>50	0.46001867298294313	7.503240032994938
>100	0.40772131007383056	19.31296924766199
>500	0.047725783810649784	7.649159250925221
>1k	0.02764870364547026	11.731149395224852
>5k	0.0018523075173311008	2.948673545032963
>10k+	6.246244625312678E-4	2.349262078814934
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	533996	0.16233440273492836	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0335975724513974E-5	0.0	0.0	2.735993574136052E-6	0.0
2	1.1855972154589559E-5	2.127995002105818E-6	0.0	2.735993574136052E-6	0.0
3	2.0367952163012833E-5	3.951990718196519E-6	0.0	3.0399928601511688E-6	3.039992860151169E-7
4	3.1919925031587276E-5	4.5599892902267535E-6	0.0	3.0399928601511688E-6	3.039992860151169E-7
5	4.347189790016172E-5	5.167987862256987E-6	0.0	3.0399928601511688E-6	3.039992860151169E-7
6	5.988785934497803E-5	5.167987862256987E-6	0.0	7.599982150377922E-6	3.039992860151169E-7
7	7.174383149956758E-5	5.471987148272104E-6	3.039992860151169E-7	8.207980722408157E-6	6.079985720302338E-7
8	8.694379580032344E-5	6.0799857203023375E-6	3.039992860151169E-7	8.511980008423273E-6	6.079985720302338E-7
9	1.0791974653536649E-4	6.383985006317455E-6	6.079985720302338E-7	9.119978580453507E-6	9.119978580453506E-7
10-11	1.4607165693026366E-4	7.75198179338548E-6	6.079985720302338E-7	9.727977152483741E-6	1.063997501052909E-6
12-13	1.8863155697238003E-4	8.511980008423273E-6	6.079985720302338E-7	1.0639975010529092E-5	1.367996787068026E-6
14-15	2.4198343166803306E-4	9.727977152483741E-6	6.079985720302338E-7	1.1399973225566883E-5	1.5199964300755844E-6
16-17	3.2315124103406926E-4	1.1247973582559324E-5	6.079985720302338E-7	1.3071969298650026E-5	1.5199964300755844E-6
18-19	4.034070525420601E-4	1.3983967156695376E-5	6.079985720302338E-7	1.3831967513687819E-5	2.127995002105818E-6
20-21	4.912628462004289E-4	1.4287966442710494E-5	9.119978580453506E-7	1.3983967156695378E-5	2.127995002105818E-6
22-23	6.054145780991053E-4	1.4895965014740727E-5	9.119978580453506E-7	1.4135966799702937E-5	2.127995002105818E-6
24-25	7.674461975451626E-4	1.5351963943763402E-5	9.119978580453506E-7	1.459196572872561E-5	2.127995002105818E-6
26-27	9.805496970417596E-4	1.7175959659854103E-5	1.063997501052909E-6	1.565596322977852E-5	2.127995002105818E-6
28-29	0.0012682850212550677	1.9151955018952365E-5	1.367996787068026E-6	1.839195680391457E-5	2.2799946451133768E-6
30-31	0.001766235851747829	2.0215952520005276E-5	1.5199964300755844E-6	1.9759953590982597E-5	2.4319942881209353E-6
32-33	0.0023882183909347586	2.1127950378050626E-5	1.671996073083143E-6	2.0975950735043066E-5	2.735993574136052E-6
34-35	0.0035200077327690385	2.1887948593088416E-5	1.8239957160907013E-6	2.249594716511865E-5	2.735993574136052E-6
36-37	0.004972668320992274	2.2951946094141324E-5	1.9759953590982596E-6	2.4167943238201793E-5	3.6479914321814026E-6
38-39	0.006391584988467832	2.4015943595194235E-5	2.4319942881209353E-6	2.7207936098352962E-5	3.951990718196519E-6
40-41	0.008362716358989851	2.4167943238201793E-5	2.735993574136052E-6	2.8575932885420988E-5	4.5599892902267535E-6
42-43	0.010545431232578392	2.568793966827738E-5	3.0399928601511688E-6	2.9791930029481457E-5	5.927986077294779E-6
44-45	0.012838193847704401	2.735993574136052E-5	3.343992146166286E-6	3.1615925745572154E-5	6.535984649325013E-6
46-47	0.015104812524233114	2.7967934313390752E-5	3.6479914321814026E-6	3.313592217564774E-5	6.991983578347688E-6
48-49	0.017214567569178024	2.8879932171436103E-5	3.951990718196519E-6	3.495991789173844E-5	7.447982507370363E-6
50-51	0.01876192393499497	3.0247928958504132E-5	4.407989647219195E-6	3.571991610677623E-5	7.90398143639304E-6
52-53	0.019704321721641833	3.2527923603617505E-5	4.5599892902267535E-6	3.739191217985938E-5	8.207980722408157E-6
54-55	0.020075048850937267	3.404792003369309E-5	4.863988576241871E-6	3.845590968091229E-5	8.967978937445948E-6
56-57	0.020231000484663024	3.6023915392791357E-5	5.167987862256987E-6	3.906390825294252E-5	9.423977866468623E-6
58-59	0.02040412807804863	3.723991253685182E-5	5.319987505264546E-6	4.05839046830181E-5	9.727977152483741E-6
60-61	0.0205704156874989	3.815191039489717E-5	5.927986077294779E-6	4.255990004211637E-5	9.879976795491299E-6
62-63	0.020751751261606916	4.103990361204078E-5	6.839983935340131E-6	4.453589540121462E-5	1.0031976438498858E-5
64-65	0.020903750904614476	4.1799901827078574E-5	7.295982864362805E-6	4.6815890046328E-5	1.0943974296544208E-5
66-67	0.021017598637227138	4.377589718617683E-5	7.75198179338548E-6	4.939988397745649E-5	1.2311971083612234E-5
68-69	0.02113235836769784	4.483989468722975E-5	7.903981436393039E-6	5.0767880764524516E-5	1.2919969655642468E-5
70-71	0.021260038067824193	4.6815890046328E-5	8.359980365415716E-6	5.2895875766630345E-5	1.3831967513687819E-5
72-73	0.021387261769021518	4.8943885048433815E-5	9.271978223461066E-6	5.6239867912796626E-5	1.4591965728725612E-5
74-75	0.021522997450227267	5.167987862256987E-5	1.0183976081506417E-5	5.8215863271894884E-5	1.5959962515793638E-5
76-77	0.021695821044326862	5.3503874338660574E-5	1.0943974296544208E-5	5.988785934497803E-5	1.732795930286166E-5
78-79	0.02194312446350016	5.6239867912796626E-5	1.0943974296544208E-5	6.201585434708384E-5	1.7935957874891896E-5
80-81	0.022328595558167328	5.867186220091756E-5	1.1399973225566883E-5	6.307985184813674E-5	1.8239957160907014E-5
82-83	0.02283353837223844	6.155985541806117E-5	1.1855972154589559E-5	6.535984649325014E-5	1.8999955375944804E-5
84-85	0.023415545005314377	6.262385291911408E-5	1.2311971083612234E-5	6.809584006738618E-5	1.9759953590982597E-5
86	0.023855431972178254	6.353585077715944E-5	1.2463970726619793E-5	6.870383863941641E-5	1.9759953590982597E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGCAGT	2087335	0.0	80.29649	1
AGCAGTG	2318255	0.0	72.06815	2
GCAGTGG	2587550	0.0	64.352844	3
CAGTGGT	2680265	0.0	61.936108	4
AGTGGTA	2753755	0.0	60.64664	5
GGTATCA	2812830	0.0	59.87685	8
TGGTATC	2800355	0.0	59.25364	7
GTGGTAT	2871305	0.0	58.11478	6
GTATCAA	2943090	0.0	57.414436	9
ACGCAGA	2820780	0.0	33.20256	14-15
CAACGCA	2847810	0.0	32.89789	12-13
AGAGTAC	2763795	0.0	32.566177	18-19
GCAGAGT	2828670	0.0	32.22891	16-17
ATCAACG	2944860	0.0	31.895077	10-11
AGTACAT	2766785	0.0	31.87564	20-21
TACATGG	2780680	0.0	31.207443	22-23
AACGCAG	2830925	0.0	30.141973	14-15
CGCAGAG	2823945	0.0	30.015764	16-17
TCAACGC	2847325	0.0	29.775257	12-13
CAGAGTA	2784015	0.0	29.53219	18-19
>>END_MODULE
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR15142072 SRR15142072_1.fastq
Input file:	SRR15142072_1.fastq
trimmed:	SRR15142072-trimmed.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- minimum overlap length for adapter detection (-k):	inf
-- number of concurrent threads (-t):	20
Tue Apr 15 05:01:33 2025 >> started

Tue Apr 15 05:04:42 2025 >> done (188.779s)
328948141 reads processed; of these:
        0 ( 0.00%) short reads filtered out after trimming by size control
        0 ( 0.00%) empty reads filtered out after trimming by size control
328948141 (100.00%) reads available; of these:
     3568 ( 0.00%) trimmed reads available after processing
328944573 (100.00%) untrimmed reads available after processing

Length distribution of reads after trimming:
length	count	percentage
 51	        1	  0.00%
 52	        1	  0.00%
 53	        0	  0.00%
 54	        1	  0.00%
 55	        0	  0.00%
 56	        0	  0.00%
 57	        1	  0.00%
 58	        0	  0.00%
 59	        0	  0.00%
 60	        0	  0.00%
 61	        0	  0.00%
 62	        1	  0.00%
 63	        1	  0.00%
 64	        0	  0.00%
 65	        1	  0.00%
 66	        0	  0.00%
 67	        0	  0.00%
 68	        0	  0.00%
 69	        0	  0.00%
 70	        0	  0.00%
 71	        0	  0.00%
 72	        0	  0.00%
 73	        0	  0.00%
 74	        0	  0.00%
 75	        0	  0.00%
 76	        0	  0.00%
 77	        0	  0.00%
 78	        0	  0.00%
 79	        0	  0.00%
 80	        0	  0.00%
 81	        0	  0.00%
 82	        0	  0.00%
 83	        0	  0.00%
 84	        0	  0.00%
 85	        0	  0.00%
 86	        0	  0.00%
 87	        0	  0.00%
 88	        0	  0.00%
 89	        0	  0.00%
 90	        0	  0.00%
 91	        0	  0.00%
 92	        0	  0.00%
 93	        0	  0.00%
 94	        0	  0.00%
 95	        0	  0.00%
 96	        0	  0.00%
 97	     3561	  0.00%
 98	328944573	100.00%
328948141 reads passed initial QC


criterion=sequence-density
sequence-density=3.19
sequence-density-rank=1
fanout-score=2.56
fanout-score-rank=44
prefix-density=8.04
prefix-fanout=1.0
sequence=GAGTACATGGGG


criterion=fanout-score
sequence-density=0.19
sequence-density-rank=33
fanout-score=39.26
fanout-score-rank=1
prefix-density=7.31
prefix-fanout=1.0
sequence=GTACATGGGAAT
Potential 3prime adapter identified. Now checking if in reference sequence
Warning: gzbuffer added in zlib v1.2.3.5. Unable to change buffer size from default of 8192.
1 reads; of these:
  1 (100.00%) were unpaired; of these:
    0 (0.00%) aligned 0 times
    0 (0.00%) aligned exactly 1 time
    1 (100.00%) aligned >1 times
100.00% overall alignment rate
Potential adapter found in reference sequence. Continuing without clipping.
                                 Started job on |	Apr 15 05:11:16
                             Started mapping on |	Apr 15 05:11:16
                                    Finished on |	Apr 15 05:19:32
       Mapping speed, Million of reads per hour |	2387.53

                          Number of input reads |	328948141
                      Average input read length |	89
                                    UNIQUE READS:
                   Uniquely mapped reads number |	286908328
                        Uniquely mapped reads % |	87.22%
                          Average mapped length |	88.10
                       Number of splices: Total |	22972883
            Number of splices: Annotated (sjdb) |	21492503
                       Number of splices: GT/AG |	22126891
                       Number of splices: GC/AG |	395902
                       Number of splices: AT/AC |	43674
               Number of splices: Non-canonical |	406416
                      Mismatch rate per base, % |	0.91%
                         Deletion rate per base |	0.03%
                        Deletion average length |	1.77
                        Insertion rate per base |	0.04%
                       Insertion average length |	1.28
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	14119478
             % of reads mapped to multiple loci |	4.29%
        Number of reads mapped to too many loci |	6312958
             % of reads mapped to too many loci |	1.92%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.54%
                     % of reads unmapped: other |	0.03%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	27920335	27920335	27920335
N_multimapping	14119478	14119478	14119478
N_noFeature	39697618	44174300	279828547
N_ambiguous	3802403	1190089	24422
UnstrandedReadsAssigned:243408307 PositiveStrandReadsAssigned:241543939 NegativeStrandReadsAssigned:7055359
Dataset is classified positive stranded
MeadianReadLen=90 20thPercentileLength=90 echo kmer=85
SRR15142072 Starting Kallisto single end mapping to ensembl reference transcriptome. kmer=31

[quant] fragment length distribution is truncated gaussian with mean = 100, sd = 20
[index] k-mer length: 31
[index] number of targets: 52,400
[index] number of k-mers: 62,057,036
[index] number of equivalence classes: 130,681
[quant] running in single-end mode
[quant] will process file 1: SRR15142072-trimmed.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 328,948,141 reads, 233,712,376 reads pseudoaligned
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,318 rounds

  52401 SRR15142072.ke.tsv
  34699 SRR15142072.se.tsv
  87100 total
==> SRR15142072.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Potri.005G200100.1.v4.1	2018	1919	9948.15	18.6574
Potri.005G024800.1.v4.1	1035	936	138964	534.33
Potri.004G059700.1.v4.1	961	862	678	2.83078
Potri.007G009000.2.v4.1	1416	1317	0	0
Potri.003G141000.2.v4.1	2943	2844	4797.64	6.07131
Potri.016G087400.1.v4.1	270	171	3679.5	77.442
Potri.015G069301.1.v4.1	564	465	0	0
Potri.010G195200.1.v4.1	1773	1674	1659.46	3.56776
Potri.012G127500.1.v4.1	977	878	295829	1212.64

==> SRR15142072.se.tsv <==
Potri.001G166300.v4.1	0
Potri.001G448400.v4.1	4019
Potri.001G233950.v4.1	27
Potri.001G122700.v4.1	20276
Potri.001G212900.v4.1	1
Potri.001G182400.v4.1	44
Potri.001G256600.v4.1	4
Potri.001G040500.v4.1	0
Potri.001G416900.v4.1	36
Potri.001G452600.v4.1	21
SRR15142072 completed mapping pipeline successfully
