Starting /dee2/code/volunteer_pipeline.sh SRR17200374 current disk space = 3049633398784 free memory = 1301595440 SRR17200374 SRAfilesize 2a8e5553d22662b65c6ac81396f848b3 SRR17200374.sra SRR17200374.sra file validated SRR17200374 is paired end SRR17200374 is conventional basespace SRR17200374 read1 length is 92-141 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR17200374_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 92-141 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.412 37.0 37.0 37.0 37.0 37.0 2 36.6515 37.0 37.0 37.0 37.0 37.0 3 36.4225 37.0 37.0 37.0 37.0 37.0 4 36.5925 37.0 37.0 37.0 37.0 37.0 5 36.381 37.0 37.0 37.0 37.0 37.0 6 36.414 37.0 37.0 37.0 37.0 37.0 7 35.9035 37.0 37.0 37.0 37.0 37.0 8 36.4015 37.0 37.0 37.0 37.0 37.0 9 36.5335 37.0 37.0 37.0 37.0 37.0 10-14 36.42 37.0 37.0 37.0 37.0 37.0 15-19 36.4139 37.0 37.0 37.0 37.0 37.0 20-24 36.3889 37.0 37.0 37.0 37.0 37.0 25-29 36.285000000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.216300000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.36 37.0 37.0 37.0 37.0 37.0 40-44 36.3584 37.0 37.0 37.0 37.0 37.0 45-49 36.31 37.0 37.0 37.0 37.0 37.0 50-54 36.273900000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.2659 37.0 37.0 37.0 37.0 37.0 60-64 36.260999999999996 37.0 37.0 37.0 37.0 37.0 65-69 36.241 37.0 37.0 37.0 37.0 37.0 70-74 36.04959999999999 37.0 37.0 37.0 37.0 37.0 75-79 35.971000000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.2354 37.0 37.0 37.0 37.0 37.0 85-89 36.2447 37.0 37.0 37.0 37.0 37.0 90-94 35.895910210210204 37.0 37.0 37.0 37.0 37.0 95-99 36.22049077539263 37.0 37.0 37.0 37.0 37.0 100-104 36.1332447415322 37.0 37.0 37.0 37.0 37.0 105-109 36.07862458243101 37.0 37.0 37.0 37.0 37.0 110-114 36.052311995825704 37.0 37.0 37.0 37.0 37.0 115-119 35.88177389990788 37.0 37.0 37.0 37.0 37.0 120-124 36.04695129982035 37.0 37.0 37.0 37.0 37.0 125-129 36.03780912230022 37.0 37.0 37.0 37.0 37.0 130-134 35.82513966480447 37.0 37.0 37.0 37.0 37.0 135-139 35.968156424581004 37.0 37.0 37.0 37.0 37.0 140-141 35.78240223463687 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 0.0 23 1.0 24 1.0 25 8.0 26 3.0 27 16.0 28 15.0 29 19.0 30 37.0 31 38.0 32 54.0 33 65.0 34 111.0 35 328.0 36 2915.0 37 388.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 18.775 8.95 41.025 31.25 2 20.05 14.075 21.8 44.074999999999996 3 26.424999999999997 21.075 19.975 32.525 4 24.224999999999998 26.3 23.025000000000002 26.450000000000003 5 20.724999999999998 28.499999999999996 26.275 24.5 6 16.75 22.95 39.625 20.674999999999997 7 18.125 18.375 35.975 27.525 8 19.125 19.575 33.125 28.175 9 21.25 31.125000000000004 24.575 23.05 10-14 22.52 24.175 26.31 26.995 15-19 23.244999999999997 23.294999999999998 26.93 26.529999999999998 20-24 23.595 23.805 25.180000000000003 27.42 25-29 23.485 23.235 26.025 27.255000000000003 30-34 23.3 22.31 26.369999999999997 28.02 35-39 22.68 23.75 25.66 27.91 40-44 22.759999999999998 23.075000000000003 26.945000000000004 27.22 45-49 22.34 23.605 26.13 27.925 50-54 22.27 23.75 26.419999999999998 27.560000000000002 55-59 21.125 24.05 27.145000000000003 27.68 60-64 23.075000000000003 23.305 26.784999999999997 26.834999999999997 65-69 22.21 23.95 26.815 27.025 70-74 22.39 24.529999999999998 25.424999999999997 27.655 75-79 22.12 24.645 25.55 27.685 80-84 22.705000000000002 23.735 25.165 28.395 85-89 22.29 23.810000000000002 26.119999999999997 27.779999999999998 90-94 22.438975590236094 24.59983993597439 25.345138055222087 27.616046418567425 95-99 22.319771233632668 24.155922339838458 26.06230873425977 27.4619976922691 100-104 21.864449483497104 25.27084908037289 25.76467624086672 27.10002519526329 105-109 21.502471841394424 24.402425972172672 26.940522909127974 27.15457927730493 110-114 22.236550265641924 25.02708000206324 26.466188683138185 26.270181049156648 115-119 22.21464182629231 23.53712936237208 26.985043295722903 27.263185515612697 120-124 23.192528581396594 25.683001449197572 25.19456819279695 25.929901776608876 125-129 22.28546167873128 24.556556335304194 25.059402110847103 28.098579875117423 130-134 22.329608938547484 23.743016759776538 26.72625698324022 27.201117318435752 135-139 21.916201117318433 24.810055865921786 25.893854748603353 27.379888268156428 140-141 23.463687150837988 25.34916201117318 25.405027932960895 25.782122905027933 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 1.0 11 1.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.0 22 1.0 23 0.5 24 0.0 25 1.5 26 3.5 27 3.5 28 1.5 29 5.0 30 9.5 31 10.5 32 13.5 33 21.5 34 31.0 35 40.0 36 54.0 37 51.0 38 48.5 39 68.0 40 84.5 41 92.0 42 101.5 43 110.0 44 142.0 45 180.0 46 182.0 47 194.0 48 195.0 49 179.5 50 154.5 51 134.5 52 155.0 53 187.5 54 208.5 55 210.0 56 199.0 57 153.0 58 130.0 59 133.0 60 113.0 61 93.0 62 74.5 63 52.5 64 37.5 65 25.5 66 15.5 67 13.5 68 13.5 69 15.0 70 11.0 71 11.0 72 12.0 73 11.5 74 6.5 75 2.5 76 2.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-141 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 92-93 4.0 94-95 4.0 96-97 11.0 98-99 4.0 100-101 7.0 102-103 11.0 104-105 22.0 106-107 24.0 108-109 21.0 110-111 14.0 112-113 19.0 114-115 29.0 116-117 37.0 118-119 24.0 120-121 40.0 122-123 53.0 124-125 32.0 126-127 45.0 128-129 19.0 130-131 0.0 132-133 0.0 134-135 0.0 136-137 0.0 138-139 0.0 140-141 3580.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.95 #Duplication Level Percentage of deduplicated Percentage of total 1 79.28118393234672 56.25 2 12.825933756166313 18.2 3 3.699788583509514 7.875 4 1.6913319238900635 4.8 5 0.7047216349541932 2.5 6 0.4580690627202256 1.95 7 0.42283298097251587 2.1 8 0.42283298097251587 2.4 9 0.10570824524312897 0.675 >10 0.3875968992248062 3.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCGAGTTATCATGAATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCTT 17 0.42500000000000004 No Hit TCGGCAATCGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGAC 13 0.325 No Hit TTCATCTTCGGCGCAAGAGCGCTCGATCAGTGAGCTATTACGCACTCTTT 13 0.325 No Hit TACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTA 12 0.3 No Hit CCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTG 12 0.3 No Hit TCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGC 12 0.3 No Hit TCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGCC 11 0.27499999999999997 No Hit CACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTAC 10 0.25 No Hit TGCGAATCAACGGTTCCTCTCGTACTAGGTTGGATTACTATTGCGACACT 10 0.25 No Hit TCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGCTATAGA 10 0.25 No Hit TCAGTATCGCTGCGGGCCTCCACCAGAGTTTCCTCTGGCTTCGCCCCGCT 10 0.25 No Hit TCAAACTTCCTTGGCCTGGAAGGCCATAGTCCCTCTAAGAAGCTGGCCGC 9 0.22499999999999998 No Hit TCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCGAGCTTTTCC 9 0.22499999999999998 No Hit CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC 9 0.22499999999999998 No Hit CTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAG 8 0.2 No Hit CCGACTTCCCTTGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTG 8 0.2 No Hit GCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGC 8 0.2 No Hit TTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATC 8 0.2 No Hit TCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATAC 8 0.2 No Hit TCCAAAAAGAGGGGCAGCGCCCCGCCTCCGATTCACGGAATAAGTAAAAT 8 0.2 No Hit TCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCACCCTCCG 8 0.2 No Hit TTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACC 8 0.2 No Hit TTCGAGCTTTTCCTGGGAGTATGGCATGGGTTACTTCAGCGCCGTAGCGC 8 0.2 No Hit CCTGTTGTCCATCGACTACGCCTTTCGGCCTGATCTTAGGCCCTGACTCA 8 0.2 No Hit ATAGAACTCGCACCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGGAA 8 0.2 No Hit GCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATTCC 8 0.2 No Hit GCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCCCC 7 0.17500000000000002 No Hit TCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGG 7 0.17500000000000002 No Hit CCCCATGCTACTCGGGTCAGAGCGTAAGCTAGTGATGCTTTCGGCTACTG 7 0.17500000000000002 No Hit TCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTT 7 0.17500000000000002 No Hit TTTACGGCTAGGACTACTGGGGTATCTAATCCCATTCGCTCCCCTAGCTT 7 0.17500000000000002 No Hit CCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCA 7 0.17500000000000002 No Hit TCAGTGTCGGCCCAGCAGAGTGCTTTCACCGTTGGTGTTCTTTCCGATCT 7 0.17500000000000002 No Hit TGGGTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTC 7 0.17500000000000002 No Hit TCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGC 7 0.17500000000000002 No Hit TCCTACTCATCGGGGCCTGGCGCTTGCCCCGACGGCCGGGTATAGGTCGC 7 0.17500000000000002 No Hit TCCAGTCAACTGCTGCGCCTCAACGCATTTCGGGGAGAACCAGCTAGCTC 7 0.17500000000000002 No Hit TCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCGCCGAAGCGGCCTTG 7 0.17500000000000002 No Hit CCACCTGTGTCGGTTTCGGGTACAGGTACCCTTTTGTTGAAGGTCGTTCG 6 0.15 No Hit GGAATTGTACTTCAAGGCGGCCCGCGCGGCTCTTTCACCGCGAGGGCTTG 6 0.15 No Hit GCATCACAGACCTGTTATTGCCTCAAACTTCCTTGGCCTGGAAGGCCATA 6 0.15 No Hit CGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCG 6 0.15 No Hit GTCTTTCTGTCCAGGTGCAGGTAGTCCGCATCTTCACAGACATGTCTATT 6 0.15 No Hit AATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCTTTTATCTAATAAAT 6 0.15 No Hit TGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGGCC 6 0.15 No Hit TCTCCCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCC 6 0.15 No Hit TGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATT 6 0.15 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 6 0.15 No Hit CAGAAATTTGAATGATGCGTCGCCAGCACGAAGGCCGTGCGATCCGTCGA 6 0.15 No Hit CCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGCTAT 6 0.15 No Hit GCAGAAATTTGAATGATGCGTCGCCAGCACGAAGGCCGTGCGATCCGTCG 6 0.15 No Hit CTACCGATGCATTTTTACATCCCACAGCTTCGGCAGATCGCTTAGCCCCG 5 0.125 No Hit CCACAACCCCGTTTTCACGGTTTAGGCTGCTCCCATTTCGCTCGCCGCTA 5 0.125 No Hit GGGTGGGCTTACTACTTAGATGCTTTCAGCAGTTATCCGCTCCGCACTTG 5 0.125 No Hit TTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCACG 5 0.125 No Hit GGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCG 5 0.125 No Hit CAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAG 5 0.125 No Hit GGTTACTCCCAGTTCTCTTTCATTGAAAGTATCATTGAAAGAAATCCTGT 5 0.125 No Hit TTCCCTCACGGTACTACTTCGCTATCGGTCACCCAGGAGTATTTAGCCTT 5 0.125 No Hit CCCTACCGATGCATTTTTACATCCCACAGCTTCGGCAGATCGCTTAGCCC 5 0.125 No Hit TAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTG 5 0.125 No Hit TTGGAACATACTACAGCCCCAGGTGGCGAAGAGCCGACATCGAGGTGCCA 5 0.125 No Hit CTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCC 5 0.125 No Hit TCGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAA 5 0.125 No Hit CCTGGTCCGCCGCTGAGGACGCTTCTCCAGACTACAATTCGGACGCCGCA 5 0.125 No Hit GGCGCACGCGTCGCATCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCA 5 0.125 No Hit TTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATTTCAA 5 0.125 No Hit TTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTA 5 0.125 No Hit TCTGCAAAGGATTCTACCCGCCGCTCGGTGGGAATTGTACTTCAAGGCGG 5 0.125 No Hit TGCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCC 5 0.125 No Hit GCCCACTTGGAGCTCTCGATTCCGTGGCGCGGCTCAACGAAGCAGCCGCG 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTACA 10 0.009125282 131.6125 2 TGTACAA 10 0.009125282 131.6125 3 TGTGTAC 10 0.009125282 131.6125 1 CAAATAC 15 0.0015148137 76.297104 132-133 >>END_MODULE SRR17200374 read2 length is 92-141 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR17200374_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 92-141 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.115 37.0 37.0 37.0 37.0 37.0 2 36.4695 37.0 37.0 37.0 37.0 37.0 3 36.4575 37.0 37.0 37.0 37.0 37.0 4 36.0975 37.0 37.0 37.0 37.0 37.0 5 36.361 37.0 37.0 37.0 37.0 37.0 6 36.3145 37.0 37.0 37.0 37.0 37.0 7 36.2575 37.0 37.0 37.0 37.0 37.0 8 36.1945 37.0 37.0 37.0 37.0 37.0 9 36.017 37.0 37.0 37.0 37.0 37.0 10-14 36.33489999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.096199999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.0596 37.0 37.0 37.0 37.0 37.0 25-29 36.1216 37.0 37.0 37.0 37.0 37.0 30-34 36.2131 37.0 37.0 37.0 37.0 37.0 35-39 36.2495 37.0 37.0 37.0 37.0 37.0 40-44 36.058899999999994 37.0 37.0 37.0 37.0 37.0 45-49 36.0955 37.0 37.0 37.0 37.0 37.0 50-54 36.2037 37.0 37.0 37.0 37.0 37.0 55-59 35.922000000000004 37.0 37.0 37.0 34.6 37.0 60-64 36.2126 37.0 37.0 37.0 37.0 37.0 65-69 35.9536 37.0 37.0 37.0 37.0 37.0 70-74 35.9795 37.0 37.0 37.0 37.0 37.0 75-79 35.6988 37.0 37.0 37.0 34.6 37.0 80-84 35.9321 37.0 37.0 37.0 34.6 37.0 85-89 35.9798 37.0 37.0 37.0 37.0 37.0 90-94 35.95632402402402 37.0 37.0 37.0 37.0 37.0 95-99 35.76115411515427 37.0 37.0 37.0 34.6 37.0 100-104 35.959991477278926 37.0 37.0 37.0 37.0 37.0 105-109 35.77144776432834 37.0 37.0 37.0 34.6 37.0 110-114 35.772998107440785 37.0 37.0 37.0 37.0 37.0 115-119 35.95805143956779 37.0 37.0 37.0 37.0 37.0 120-124 35.90423832916505 37.0 37.0 37.0 37.0 37.0 125-129 35.6083408403603 37.0 37.0 37.0 34.6 37.0 130-134 35.72605417481151 37.0 37.0 37.0 37.0 37.0 135-139 35.84367495113097 37.0 37.0 37.0 37.0 37.0 140-141 35.63557665456577 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 1.0 23 1.0 24 1.0 25 1.0 26 7.0 27 7.0 28 20.0 29 18.0 30 23.0 31 34.0 32 66.0 33 98.0 34 167.0 35 645.0 36 2695.0 37 214.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 25.324999999999996 23.95 32.675 18.05 2 22.45 25.074999999999996 28.4 24.075 3 27.675 34.425 19.675 18.224999999999998 4 29.25 36.5 18.4 15.85 5 21.6 37.625 22.7 18.075 6 23.425 21.45 32.975 22.15 7 22.5 24.025 27.175 26.3 8 25.75 23.9 26.150000000000002 24.2 9 25.624999999999996 33.025 20.724999999999998 20.625 10-14 28.24 25.345000000000002 23.56 22.855 15-19 27.765 26.295 23.715 22.225 20-24 27.084999999999997 25.974999999999998 24.52 22.42 25-29 27.515 25.955000000000002 24.075 22.455 30-34 27.18 27.165 23.87 21.785 35-39 26.375 27.57 23.48 22.575 40-44 27.075 26.72 24.474999999999998 21.73 45-49 27.169999999999998 26.919999999999998 23.74 22.17 50-54 28.435 25.77 24.39 21.404999999999998 55-59 27.22 26.340000000000003 24.474999999999998 21.965 60-64 27.99 25.419999999999998 24.02 22.57 65-69 27.165 25.47 24.834999999999997 22.53 70-74 27.245 26.275 23.665 22.814999999999998 75-79 28.194999999999997 25.61 24.19 22.005 80-84 27.005000000000003 25.480000000000004 24.2 23.315 85-89 27.395000000000003 25.319999999999997 24.055 23.23 90-94 27.475990396158462 25.950380152060827 23.934573829531814 22.6390556222489 95-99 27.43189685446245 26.829880098329404 23.318115687553302 22.420107359654846 100-104 27.40740740740741 26.792642983119176 23.6130007558579 22.186948853615522 105-109 27.151811649594865 25.979717678234728 23.742547011160372 23.125923661010038 110-114 27.511344884488448 26.69657590759076 23.65924092409241 22.13283828382838 115-119 26.86778593913956 26.726128016789087 23.05876180482686 23.34732423924449 120-124 26.54572778016316 26.29347359381709 24.028553027050236 23.132245598969515 125-129 27.57915676631486 26.496104326683984 23.584019450737692 22.340719456263468 130-134 27.428092711533093 27.05389555989947 23.077352694777993 22.440659033789444 135-139 27.456017872102766 26.607092990784697 23.351019268360794 22.585869868751747 140-141 27.240994135716278 27.85534766824909 22.21446523317509 22.689192962859536 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.0 22 0.5 23 0.5 24 1.0 25 1.0 26 2.5 27 3.5 28 2.5 29 4.5 30 7.5 31 5.5 32 10.0 33 18.0 34 25.5 35 33.5 36 47.5 37 61.0 38 66.0 39 87.5 40 104.5 41 104.5 42 114.5 43 127.0 44 154.5 45 169.0 46 185.0 47 202.0 48 199.5 49 191.0 50 175.5 51 163.0 52 172.5 53 192.5 54 188.5 55 188.0 56 182.0 57 161.0 58 126.5 59 103.0 60 93.5 61 78.5 62 62.0 63 40.5 64 22.5 65 14.5 66 13.5 67 12.0 68 15.5 69 17.5 70 15.5 71 15.0 72 8.0 73 5.5 74 3.0 75 0.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-141 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 92-93 4.0 94-95 4.0 96-97 11.0 98-99 4.0 100-101 7.0 102-103 11.0 104-105 22.0 106-107 24.0 108-109 20.0 110-111 14.0 112-113 19.0 114-115 29.0 116-117 37.0 118-119 24.0 120-121 40.0 122-123 55.0 124-125 31.0 126-127 45.0 128-129 18.0 130-131 0.0 132-133 0.0 134-135 0.0 136-137 0.0 138-139 0.0 140-141 3581.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.125 #Duplication Level Percentage of deduplicated Percentage of total 1 79.62900505902192 59.025000000000006 2 13.355817875210793 19.8 3 3.5075885328836423 7.8 4 1.9561551433389546 5.800000000000001 5 0.6070826306913997 2.25 6 0.5059021922428331 2.25 7 0.1011804384485666 0.525 8 0.1011804384485666 0.6 9 0.06745362563237774 0.44999999999999996 >10 0.16863406408094433 1.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGGGAGTAACTAT 13 0.325 No Hit TCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGGA 13 0.325 No Hit CCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACG 12 0.3 No Hit TAATTCTAGAGCTAATACGTGCAACAAACCCCGACTTCTGGAAGGGACGC 11 0.27499999999999997 No Hit TTAGTTTTACCCTACTGATGACAGTGTCGCAATAGTAATCCAACCTAGTA 11 0.27499999999999997 No Hit TTCGAGTGAGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTATGCCT 9 0.22499999999999998 No Hit TTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCGCACG 9 0.22499999999999998 No Hit CTCTTTCTTGATTCTATGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGG 8 0.2 No Hit GCTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGC 8 0.2 No Hit GAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTTCTGGGCCG 8 0.2 No Hit TTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCGA 7 0.17500000000000002 No Hit GGAAAGGTTCCATGTGAACGGCACTTGCACATGGGTTAGTCGATCCTAAG 7 0.17500000000000002 No Hit CTAAATACGGGCGAGAGACCGATAGCAAACAAGTACCGCGAGGGAAAGAT 7 0.17500000000000002 No Hit TCTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTA 6 0.15 No Hit TCAGAATCCGGGCTAGATGCGACGCGTGCGCCCGCCGTCCGATTGCCGAC 6 0.15 No Hit TCTATAGCCTTGGCCGACAGGCCCGGGTAATCTTTGAAATTTCATCGTGA 6 0.15 No Hit CGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACT 6 0.15 No Hit AGGAACCGTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAG 6 0.15 No Hit TTCGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAACACATGCAAG 6 0.15 No Hit CATCGGGGGCGCAACGCCCTCGACCTATTCTCAAACTTTAAATAGGTAGG 6 0.15 No Hit ACTACTTTTAACGTTATTTTACTTATTCCGTGAATCGGAGGCGGGGCGCT 6 0.15 No Hit TTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGA 6 0.15 No Hit ATTAACAGGGACAGTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAAT 6 0.15 No Hit GAATTTCCGGTGGAGCGGTGAAATGCGTAGAGATCGGAAAGAACACCAAC 6 0.15 No Hit GAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAGC 6 0.15 No Hit TGCGTAATAGCTCACTGATCGAGCGCTCTTGCGCCGAAGATGAACGGGGC 6 0.15 No Hit CCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGATT 6 0.15 No Hit CTTACGGTGGATACCTAGGCACCCAGAGACGAGGAAGGGCGTAGTAAGCG 6 0.15 No Hit TCGGCCAGTGAGACGGTGGGGGATAAGCTTCATCGTCGAGAGGGAAACAG 5 0.125 No Hit TCGCAATAGTAATCCAACCTAGTACGAGAGGAACCGTTGATTCGCACAAT 5 0.125 No Hit TCGACGGATCGCACGGCCTTCGTGCTGGCGACGCATCATTCAAATTTCTG 5 0.125 No Hit GGAGGAGCCCGGGGCTCTGACCGCGTGCCTGTTGAAGAATGAGCCGGCGA 5 0.125 No Hit GACGGGCTGGGAACGGCCCCTTCGGGGGCCTTCCCCGGGCGTCGAACAGT 5 0.125 No Hit GGAGAGCAATACAAGCGTCGTGCTGCTAGGCGAAGCGGTGGAGTGCTGCA 5 0.125 No Hit ATTAATCAAGAACGAAAGTTGGGGGCTCGAAGACGATCAGATACCGTCCT 5 0.125 No Hit GAAAAGAGTAGCACTTCGAACGGGACCACTTGCTTAGCTTCATCTTCTGT 5 0.125 No Hit CTTCTTAGAGGGACTATGGCCTTCCAGGCCAAGGAAGTTTGAGGCAATAA 5 0.125 No Hit TATGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGTGAGCTGG 5 0.125 No Hit TTCTATCGGGTAAAGCCAATGATTAGAGGCATCGGGGGCGCAACGCCCTC 5 0.125 No Hit GGAGCGTTCCGCTTAGAGGGAAGCACCCGCGCGAGCGGGGGTGGACGAAG 5 0.125 No Hit CCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTAT 5 0.125 No Hit CCAGTGGCGCGAAGCTACCGTGCGTTGGATTATGACTGAACGCCTCTAAG 5 0.125 No Hit TTGAGGCGCAGCAGTTGACTGGACATCTAGGGGTAAAGCACTGTTTCGGT 5 0.125 No Hit GCGAATATTCCTGTACTACCCCTTGTTGGTCCCGAGGGACGGAGGAGGCT 5 0.125 No Hit TGAAATGCCACTCGAACCCAGAGCTAGCTGGTTCTCCCCGAAATGCGTTG 5 0.125 No Hit TGGGAGCTTCGGCGAAGGTGAAATACCACTACTTTTAACGTTATTTTACT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGAGCT 10 0.009125282 131.6125 7 ATATCAA 10 0.009125282 131.6125 8 GGAGCTT 10 0.009125282 131.6125 8 CACCCAG 20 0.008558555 49.354687 20-21 >>END_MODULE Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397664 spots for SRR17200374.sra Written 1397664 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra Read 1397650 spots for SRR17200374.sra Written 1397650 spots for SRR17200374.sra SRR ids: ['SRR17200374.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_6vrfx495 SRR17200374.sra spots: 27953014 blocks: [[1, 1397650], [1397651, 2795300], [2795301, 4192950], [4192951, 5590600], [5590601, 6988250], [6988251, 8385900], [8385901, 9783550], [9783551, 11181200], [11181201, 12578850], [12578851, 13976500], [13976501, 15374150], [15374151, 16771800], [16771801, 18169450], [18169451, 19567100], [19567101, 20964750], [20964751, 22362400], [22362401, 23760050], [23760051, 25157700], [25157701, 26555350], [26555351, 27953014]] SRR17200374 file size 8790672 SRR17200374 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR17200374 SRR17200374_1.fastq SRR17200374_2.fastq Input file: SRR17200374_1.fastq Paired file: SRR17200374_2.fastq trimmed: SRR17200374-trimmed-pair1.fastq, SRR17200374-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 05:43:00 2025 >> started Wed Feb 12 05:43:31 2025 >> done (31.180s) 27953014 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 27953014 (100.00%) read pairs available; of these: 74701 ( 0.27%) trimmed read pairs available after processing 27878313 (99.73%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 89 354 0.00% 90 340 0.00% 91 399 0.00% 92 17196 0.06% 93 19733 0.07% 94 22170 0.08% 95 23683 0.08% 96 27214 0.10% 97 28266 0.10% 98 30495 0.11% 99 34269 0.12% 100 36510 0.13% 101 40065 0.14% 102 44313 0.16% 103 46842 0.17% 104 49931 0.18% 105 53474 0.19% 106 55404 0.20% 107 58088 0.21% 108 62949 0.23% 109 67001 0.24% 110 70946 0.25% 111 77638 0.28% 112 82461 0.29% 113 82780 0.30% 114 86126 0.31% 115 96032 0.34% 116 99626 0.36% 117 101776 0.36% 118 107630 0.39% 119 112046 0.40% 120 109426 0.39% 121 117921 0.42% 122 122480 0.44% 123 130424 0.47% 124 128032 0.46% 125 136781 0.49% 126 140285 0.50% 127 145455 0.52% 128 152713 0.55% 129 1699 0.01% 130 7022 0.03% 131 4187 0.01% 132 6575 0.02% 133 8383 0.03% 134 44632 0.16% 135 0 0.00% 136 242 0.00% 137 597 0.00% 138 3021 0.01% 139 4820 0.02% 140 18344 0.07% 141 25034218 89.56% 27953014 reads passed initial QC criterion=sequence-density sequence-density=1.78 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=27 prefix-density=1.77 prefix-fanout=2.0 sequence=TCCTCTCGTACTAGGGAAAGGTCCTCTCAATGCTCTAACGCCCACACCGGATATGGACCGAACTGTCTCACGA criterion=fanout-score sequence-density=0.34 sequence-density-rank=20 fanout-score=16.05 fanout-score-rank=1 prefix-density=2.10 prefix-fanout=2.6 sequence=CGCATCGCCGGCCCCCATCCACTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCAGACAGCGCCTCGTGGTGCGGCAGGGTCCAGCCACGACGGGGCTCTCACCCTCTCCGGCGCCCCTTTCCAGGGGACTTGGGCCTGGTCCGCCGCTGAGGACGCTTCTCCAGACTACAATTCGGACGCCGCAGGCGCCAGATTCTCAAGCTGGGCATTTCCCGGTTCGCTCGCCGTTACTAGGGGAATCCTTGTAAGTTTCTTTTCCT criterion=sequence-density sequence-density=1.83 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=26 prefix-density=1.88 prefix-fanout=2.0 sequence=CCGAAAGATGGT criterion=fanout-score sequence-density=0.25 sequence-density-rank=24 fanout-score=6.77 fanout-score-rank=1 prefix-density=1.06 prefix-fanout=1.6 sequence=CGGCGATGCGCCCCGGTCGGATGTGGAACGGTTACAGCCGGTCCGCCGATCGGCTCGGGGCGTGGACCGATGCGGATCGCGGTGGCGGCCCAAGCCCGGGCCTTTGAAACGCCCGCGGAGACGCCGTCGTCGCGATCGTGGACTGCAG SRR17200374 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 05:44:26 Started mapping on | Feb 12 05:44:26 Finished on | Feb 12 05:49:18 Mapping speed, Million of reads per hour | 344.63 Number of input reads | 27953014 Average input read length | 276 UNIQUE READS: Uniquely mapped reads number | 13653257 Uniquely mapped reads % | 48.84% Average mapped length | 277.90 Number of splices: Total | 8330245 Number of splices: Annotated (sjdb) | 8176233 Number of splices: GT/AG | 8133808 Number of splices: GC/AG | 151360 Number of splices: AT/AC | 6249 Number of splices: Non-canonical | 38828 Mismatch rate per base, % | 0.62% Deletion rate per base | 0.02% Deletion average length | 1.69 Insertion rate per base | 0.09% Insertion average length | 4.08 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1339125 % of reads mapped to multiple loci | 4.79% Number of reads mapped to too many loci | 7870300 % of reads mapped to too many loci | 28.16% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 14.75% % of reads unmapped: other | 3.46% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 12960632 12960632 12960632 N_multimapping 1339125 1339125 1339125 N_noFeature 3699524 13208929 3765836 N_ambiguous 495786 2871 115838 UnstrandedReadsAssigned:9457947 PositiveStrandReadsAssigned:441457 NegativeStrandReadsAssigned:9771583 Dataset is classified negative stranded MeadianReadLen=141 20thPercentileLength=141 echo kmer=137 SRR17200374 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR17200374-trimmed-pair1.fastq SRR17200374-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 27,953,014 reads, 17,751,603 reads pseudoaligned [quant] estimated average fragment length: 180.481 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,075 rounds 52401 SRR17200374.ke.tsv 34699 SRR17200374.se.tsv 87100 total ==> SRR17200374.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1838.52 220 3.5645 Potri.005G024800.1.v4.1 1035 855.519 92 3.20334 Potri.004G059700.1.v4.1 961 781.536 21 0.800414 Potri.007G009000.2.v4.1 1416 1236.52 1 0.0240904 Potri.003G141000.2.v4.1 2943 2763.52 428 4.61345 Potri.016G087400.1.v4.1 270 101.112 340 100.166 Potri.015G069301.1.v4.1 564 384.59 0 0 Potri.010G195200.1.v4.1 1773 1593.52 2 0.0373867 Potri.012G127500.1.v4.1 977 797.524 264 9.86062 ==> SRR17200374.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 92 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 154 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 134 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1 SRR17200374 completed mapping pipeline successfully