Starting /dee2/code/volunteer_pipeline.sh SRR18274410 current disk space = 3056984014848 free memory = 1052988576 SRR18274410 SRAfilesize d9523d9d699dab6f9ba19deba76b92ad SRR18274410.sra SRR18274410.sra file validated SRR18274410 is paired end SRR18274410 is conventional basespace SRR18274410 read1 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR18274410_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3125 32.0 32.0 32.0 32.0 32.0 2 30.4825 32.0 32.0 32.0 32.0 32.0 3 34.96375 37.0 32.0 37.0 32.0 37.0 4 35.70875 37.0 37.0 37.0 32.0 37.0 5 36.01625 37.0 37.0 37.0 32.0 37.0 6 39.26875 41.0 41.0 41.0 37.0 41.0 7 39.43375 41.0 41.0 41.0 37.0 41.0 8 39.50625 41.0 41.0 41.0 37.0 41.0 9 39.58075 41.0 41.0 41.0 37.0 41.0 10-14 39.541450000000005 41.0 41.0 41.0 37.0 41.0 15-19 39.467499999999994 41.0 41.0 41.0 37.0 41.0 20-24 39.261 41.0 41.0 41.0 37.0 41.0 25-29 39.26925 41.0 41.0 41.0 37.0 41.0 30-34 39.1421 41.0 41.0 41.0 37.0 41.0 35-39 39.0605 41.0 41.0 41.0 37.0 41.0 40-44 38.89255 41.0 41.0 41.0 34.0 41.0 45-49 38.8306 41.0 41.0 41.0 34.0 41.0 50-54 38.765499999999996 41.0 41.0 41.0 34.0 41.0 55-59 38.7192 41.0 41.0 41.0 32.0 41.0 60-64 38.691199999999995 41.0 41.0 41.0 32.0 41.0 65-69 38.488099999999996 41.0 41.0 41.0 32.0 41.0 70-74 38.436 41.0 41.0 41.0 32.0 41.0 75-79 38.2825 41.0 40.2 41.0 31.0 41.0 80-84 38.629 41.0 41.0 41.0 32.0 41.0 85-89 38.8268 41.0 41.0 41.0 32.0 41.0 90-94 38.66135 41.0 41.0 41.0 32.0 41.0 95-99 38.5235 41.0 41.0 41.0 32.0 41.0 100-104 38.37145 41.0 41.0 41.0 32.0 41.0 105-109 38.52105 41.0 41.0 41.0 32.0 41.0 110-114 38.28205 41.0 41.0 41.0 32.0 41.0 115-119 38.10565 41.0 41.0 41.0 31.0 41.0 120-124 38.136 41.0 41.0 41.0 32.0 41.0 125-129 38.11415 41.0 41.0 41.0 31.0 41.0 130-134 37.567949999999996 41.0 37.0 41.0 26.0 41.0 135-139 37.76245 41.0 37.0 41.0 29.0 41.0 140-144 37.4549 41.0 37.0 41.0 27.0 41.0 145-149 37.1991 41.0 37.0 41.0 27.0 41.0 150 37.165 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.10412676523915465 1101 2 -0.9192650476263431 1101 3 0.39288541586864056 1101 4 0.364986337751219 1101 5 0.21642534283306247 1101 6 0.16717740493884037 1101 7 0.3727495594882413 1101 8 -0.4900022983222243 1101 9 0.2646007303557312 1101 10-14 -0.008585510355217707 1101 15-19 -0.001138946346948444 1101 20-24 -0.00856252713297323 1101 25-29 0.2368395515717907 1101 30-34 0.0389565617099521 1101 35-39 -0.048813810362879906 1101 40-44 -0.31797287979775035 1101 45-49 -0.058203733496767995 1101 50-54 0.055795602543476264 1101 55-59 -0.06262161955106649 1101 60-64 -0.06390357261421542 1101 65-69 -0.21680328915446978 1101 70-74 -0.0626829081437208 1101 75-79 -0.2852192344033284 1101 80-84 -0.21228070175438063 1101 85-89 -0.11098598023443174 1101 90-94 0.009458872800628626 1101 95-99 -0.2759314589238784 1101 100-104 -0.2587144717689469 1101 105-109 -0.024270282693628076 1101 110-114 -0.23891059526545888 1101 115-119 -0.08202201281952881 1101 120-124 0.3276386015986077 1101 125-129 0.08222886181975753 1101 130-134 -0.44140810541637876 1101 135-139 -0.05874766975663448 1101 140-144 -0.31256416149544464 1101 145-149 -0.03163512857835826 1101 150 -0.5271968129931821 1102 1 -0.1041267652391511 1102 2 0.9192650476263431 1102 3 -0.39288541586863346 1102 4 -0.364986337751219 1102 5 -0.21642534283306958 1102 6 -0.16717740493884037 1102 7 -0.3727495594882413 1102 8 0.4900022983222243 1102 9 -0.2646007303557312 1102 10-14 0.008585510355217707 1102 15-19 0.0011389463469413386 1102 20-24 0.00856252713297323 1102 25-29 -0.2368395515717978 1102 30-34 -0.03895656170994499 1102 35-39 0.0488138103628728 1102 40-44 0.31797287979775035 1102 45-49 0.0582037334967751 1102 50-54 -0.055795602543476264 1102 55-59 0.06262161955105938 1102 60-64 0.06390357261421542 1102 65-69 0.21680328915446978 1102 70-74 0.0626829081437208 1102 75-79 0.2852192344033213 1102 80-84 0.21228070175438774 1102 85-89 0.11098598023443174 1102 90-94 -0.009458872800628626 1102 95-99 0.2759314589238784 1102 100-104 0.2587144717689398 1102 105-109 0.02427028269363518 1102 110-114 0.23891059526545888 1102 115-119 0.08202201281952881 1102 120-124 -0.3276386015986148 1102 125-129 -0.08222886181976463 1102 130-134 0.44140810541638587 1102 135-139 0.058747669756641585 1102 140-144 0.31256416149544464 1102 145-149 0.03163512857836537 1102 150 0.5271968129931821 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 3.0 17 6.0 18 7.0 19 12.0 20 23.0 21 14.0 22 17.0 23 19.0 24 17.0 25 19.0 26 23.0 27 32.0 28 32.0 29 29.0 30 42.0 31 56.0 32 70.0 33 79.0 34 65.0 35 93.0 36 128.0 37 195.0 38 235.0 39 415.0 40 2369.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 14.814814814814813 29.30209120685311 14.814814814814813 41.06827916351726 2 20.010327911179964 22.773044151820294 38.23909114381616 18.977536793183578 3 17.775 28.199999999999996 29.15 24.875 4 20.674999999999997 24.474999999999998 29.25 25.6 5 26.450000000000003 23.674999999999997 28.175 21.7 6 23.075000000000003 26.400000000000002 26.5 24.025 7 23.775 26.1 26.025 24.099999999999998 8 19.675 22.475 33.4 24.45 9 21.349999999999998 22.125 33.0 23.525 10-14 23.330000000000002 25.650000000000002 26.534999999999997 24.485 15-19 24.245 25.045 25.72 24.990000000000002 20-24 24.255 24.68 26.035000000000004 25.03 25-29 23.75 24.73 26.525 24.995 30-34 24.435000000000002 25.035 26.14 24.39 35-39 24.39 25.290000000000003 25.130000000000003 25.19 40-44 24.64 25.36 25.47 24.529999999999998 45-49 24.245 25.77 25.224999999999998 24.759999999999998 50-54 24.145 25.324999999999996 25.755 24.775 55-59 24.99874981247187 24.208631294694204 25.763864579686953 25.028754313146973 60-64 24.955 23.47 26.525 25.05 65-69 24.675 25.650000000000002 25.025 24.65 70-74 24.43 24.705 25.85 25.014999999999997 75-79 25.145 24.57 24.95 25.335 80-84 24.335 24.815 25.27 25.580000000000002 85-89 24.545 26.064999999999998 25.064999999999998 24.325 90-94 24.505 25.91 25.355 24.23 95-99 25.485000000000003 25.240000000000002 25.095 24.18 100-104 24.58114528632158 25.22630657664416 25.61140285071268 24.58114528632158 105-109 24.495 24.884999999999998 25.46 25.16 110-114 25.06375956393459 24.783717557633643 25.2737910686603 24.878731809771466 115-119 25.007502250675202 25.002500750225064 25.292587776332898 24.69740922276683 120-124 24.776194048512128 25.18129532383096 25.32133033258315 24.72118029507377 125-129 25.115 25.8 25.195 23.89 130-134 24.765 24.785 26.125 24.325 135-139 24.305 25.585 25.840000000000003 24.27 140-144 25.465 26.400000000000002 24.82 23.315 145-149 25.435000000000002 26.71 24.665 23.189999999999998 150 25.6 26.525 25.3 22.575 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 1.5 2 3.0 3 2.5 4 1.5 5 1.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 1.0 12 1.5 13 0.5 14 0.5 15 1.0 16 1.5 17 1.5 18 1.5 19 4.5 20 5.0 21 3.0 22 2.5 23 3.0 24 3.5 25 3.0 26 3.0 27 7.0 28 10.5 29 11.0 30 12.5 31 16.5 32 18.5 33 22.0 34 35.0 35 43.0 36 48.0 37 54.5 38 61.5 39 78.0 40 98.0 41 121.0 42 133.0 43 154.5 44 207.0 45 237.5 46 198.5 47 168.0 48 166.0 49 167.0 50 165.0 51 140.0 52 138.5 53 147.5 54 142.0 55 121.0 56 114.5 57 120.5 58 101.5 59 107.5 60 112.5 61 76.5 62 54.0 63 57.0 64 46.0 65 29.5 66 30.0 67 33.5 68 30.5 69 24.0 70 19.5 71 15.5 72 13.5 73 8.5 74 6.0 75 4.5 76 3.5 77 9.5 78 9.5 79 2.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.775 2 3.175 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.015 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.025 105-109 0.0 110-114 0.015 115-119 0.03 120-124 0.025 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.975 #Duplication Level Percentage of deduplicated Percentage of total 1 88.40835539864666 75.125 2 7.914092380111798 13.450000000000001 3 2.2947925860547222 5.8500000000000005 4 0.7649308620182407 2.6 5 0.44130626654898497 1.875 6 0.02942041776993233 0.15 7 0.088261253309797 0.525 8 0.02942041776993233 0.2 9 0.02942041776993233 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTATGAAATACGAATGCCCCCGACTGTCCCTGTTAATCATTACTCCGATC 9 0.22499999999999998 No Hit CCCTCTTTTTGGACCCAAGGCCGCTTCGGCGGCCGATCCGGGCGGAAGAC 8 0.2 No Hit CTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACAC 7 0.17500000000000002 No Hit ATCGGAGGCGGGGCGCTGCCCCTCTTTTTGGACCCAAGGCCGCTTCGGCG 7 0.17500000000000002 No Hit GTTATCTTTTAACAGATGTGCCGCCCCAGCCAAACTCCCCACCTGACAAT 7 0.17500000000000002 No Hit CTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT 6 0.15 No Hit CATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATG 5 0.125 No Hit CAATGTCTTCCGCCCGGATCGGCCGCCGAAGCGGCCTTGGGTCCAAAAAG 5 0.125 No Hit CTCTTTTTGGACCCAAGGCCGCTTCGGCGGCCGATCCGGGCGGAAGACAT 5 0.125 No Hit ATCTTTTAACAGATGTGCCGCCCCAGCCAAACTCCCCACCTGACAATGTC 5 0.125 No Hit ATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATTTCTGTT 5 0.125 No Hit AATCGGGTTAAAATTCCTGAACCGGGACGTGGCGGCTGACGGCAACGTTA 5 0.125 No Hit CTTATTCCGTGAATCGGAGGCGGGGCGCTGCCCCTCTTTTTGGACCCAAG 5 0.125 No Hit CTTTGTGTTTGACACCTCTAGCTTCAAATTCCGAAGGTCTAAAGGATCGA 5 0.125 No Hit ATACGAATGCCCCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGG 5 0.125 No Hit CCCCTCTTTTTGGACCCAAGGCCGCTTCGGCGGCCGATCCGGGCGGAAGA 5 0.125 No Hit CCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAA 5 0.125 No Hit CTACTTTTAACGTTATTTTACTTATTCCGTGAATCGGAGGCGGGGCGCTG 5 0.125 No Hit TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit CTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGAT 5 0.125 No Hit AAAGGGAATCGGGTTAAAATTCCTGAACCGGGACGTGGCGGCTGACGGCA 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0125 0.0 0.0 0.0 106-107 0.0 0.025 0.0 0.0 0.0 108-109 0.0 0.025 0.0 0.0 0.0 110-111 0.0 0.025 0.0 0.0 0.0 112-113 0.0 0.025 0.0 0.0 0.0 114-115 0.0 0.025 0.0 0.0 0.0 116-117 0.0 0.025 0.0 0.0 0.0 118-119 0.0 0.025 0.0 0.0 0.0 120-121 0.0 0.025 0.0 0.0 0.0 122-123 0.0 0.025 0.0 0.0 0.0 124-125 0.0 0.025 0.0 0.0 0.0 126-127 0.0 0.025 0.0 0.0 0.0 128-129 0.0 0.025 0.0 0.0 0.0 130-131 0.0 0.025 0.0 0.0 0.0 132-133 0.0 0.025 0.0 0.0 0.0 134-135 0.0 0.025 0.0 0.0 0.0 136-137 0.0 0.025 0.0 0.0 0.0 138 0.0 0.025 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTTTGA 35 2.7863898E-5 84.24176 1 >>END_MODULE SRR18274410 read2 length is 150 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR18274410_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 150 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.08 32.0 27.0 32.0 2.0 32.0 2 30.71125 32.0 32.0 32.0 32.0 32.0 3 33.265 37.0 32.0 37.0 32.0 37.0 4 34.25 37.0 32.0 37.0 27.0 37.0 5 34.71875 37.0 37.0 37.0 32.0 37.0 6 37.53875 41.0 37.0 41.0 32.0 41.0 7 38.06125 41.0 37.0 41.0 32.0 41.0 8 38.11625 41.0 37.0 41.0 32.0 41.0 9 37.7975 41.0 37.0 41.0 27.0 41.0 10-14 38.373000000000005 41.0 41.0 41.0 32.0 41.0 15-19 38.1576 41.0 39.4 41.0 31.0 41.0 20-24 37.999550000000006 41.0 37.8 41.0 29.0 41.0 25-29 38.07315 41.0 39.4 41.0 30.0 41.0 30-34 37.772850000000005 41.0 37.0 41.0 28.0 41.0 35-39 37.726549999999996 41.0 37.0 41.0 27.0 41.0 40-44 37.84134999999999 41.0 37.8 41.0 27.0 41.0 45-49 37.83215 41.0 37.0 41.0 27.0 41.0 50-54 37.77645 41.0 37.0 41.0 27.0 41.0 55-59 37.509750000000004 41.0 37.0 41.0 27.0 41.0 60-64 37.24875 41.0 37.0 41.0 27.0 41.0 65-69 37.73685 41.0 37.8 41.0 27.0 41.0 70-74 38.20405000000001 41.0 41.0 41.0 32.0 41.0 75-79 37.5505 41.0 37.8 41.0 28.0 41.0 80-84 37.936350000000004 41.0 39.4 41.0 29.0 41.0 85-89 37.92265 41.0 39.4 41.0 29.0 41.0 90-94 37.910799999999995 41.0 39.4 41.0 28.0 41.0 95-99 37.89405 41.0 38.6 41.0 30.0 41.0 100-104 37.623000000000005 41.0 37.0 41.0 27.0 41.0 105-109 37.7654 41.0 37.0 41.0 28.0 41.0 110-114 37.686299999999996 41.0 37.0 41.0 28.0 41.0 115-119 37.3284 41.0 37.0 41.0 27.0 41.0 120-124 36.86919999999999 41.0 37.0 41.0 25.0 41.0 125-129 36.77925 41.0 37.0 41.0 24.0 41.0 130-134 36.7608 41.0 37.0 41.0 23.0 41.0 135-139 36.48965 41.0 37.0 41.0 22.0 41.0 140-144 36.06075 41.0 37.0 41.0 22.0 41.0 145-149 35.56085 41.0 33.0 41.0 22.0 41.0 150 35.65125 41.0 32.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -6.735233279705817 1101 2 0.1033606578308941 1101 3 -1.0219234403330049 1101 4 0.23168364871421687 1101 5 0.6365714139789063 1101 6 -0.26915906943487045 1101 7 0.10288822492913852 1101 8 0.15144666615592683 1101 9 -0.033415051456884726 1101 10-14 -0.021187977221075016 1101 15-19 0.04680660895323996 1101 20-24 0.30146837253249714 1101 25-29 -0.07403151255139306 1101 30-34 0.34871932378252524 1101 35-39 0.06282080747720897 1101 40-44 0.13159171582522333 1101 45-49 -0.0806302510278627 1101 50-54 0.4546336729742819 1101 55-59 0.2520033708725933 1101 60-64 0.6487908271406297 1101 65-69 0.47172808294389057 1101 70-74 0.3773717408514017 1101 75-79 -0.15204933731709502 1101 80-84 0.21743405092060897 1101 85-89 -0.24026405168671516 1101 90-94 0.1250491585587028 1101 95-99 -0.0021910671876170795 1101 100-104 -0.19931816440664818 1101 105-109 -0.12749814857376407 1101 110-114 -0.04561148139635662 1101 115-119 -0.054585152838427575 1101 120-124 -0.23808575295589662 1101 125-129 -0.027145739165959526 1101 130-134 0.08584233509537853 1101 135-139 0.01701524553742928 1101 140-144 0.18992058019868097 1101 145-149 0.028882249291342532 1101 150 0.13491151459434292 1102 1 6.735233279705813 1102 2 -0.1033606578308941 1102 3 1.0219234403329978 1102 4 -0.23168364871421687 1102 5 -0.6365714139789063 1102 6 0.26915906943487045 1102 7 -0.10288822492913852 1102 8 -0.15144666615592683 1102 9 0.03341505145687762 1102 10-14 0.021187977221075016 1102 15-19 -0.04680660895323996 1102 20-24 -0.30146837253249714 1102 25-29 0.07403151255139306 1102 30-34 -0.34871932378252524 1102 35-39 -0.06282080747720897 1102 40-44 -0.13159171582522333 1102 45-49 0.0806302510278627 1102 50-54 -0.4546336729742819 1102 55-59 -0.2520033708726004 1102 60-64 -0.6487908271406297 1102 65-69 -0.4717280829438977 1102 70-74 -0.3773717408514017 1102 75-79 0.15204933731709502 1102 80-84 -0.21743405092060897 1102 85-89 0.24026405168671516 1102 90-94 -0.1250491585587028 1102 95-99 0.002191067187624185 1102 100-104 0.19931816440664818 1102 105-109 0.12749814857376407 1102 110-114 0.04561148139636373 1102 115-119 0.054585152838427575 1102 120-124 0.23808575295589662 1102 125-129 0.02714573916596663 1102 130-134 -0.08584233509537853 1102 135-139 -0.01701524553742928 1102 140-144 -0.18992058019867386 1102 145-149 -0.028882249291349638 1102 150 -0.13491151459435002 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 3.0 15 2.0 16 3.0 17 7.0 18 9.0 19 12.0 20 22.0 21 23.0 22 31.0 23 28.0 24 34.0 25 23.0 26 38.0 27 59.0 28 59.0 29 50.0 30 76.0 31 79.0 32 89.0 33 90.0 34 113.0 35 150.0 36 162.0 37 227.0 38 312.0 39 492.0 40 1807.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 15.274759391493326 29.74231605091586 15.429990686122322 39.55293387146849 2 21.05 24.525 37.2 17.224999999999998 3 18.925 27.950000000000003 28.15 24.975 4 21.925 25.025 28.799999999999997 24.25 5 26.125 25.724999999999998 24.65 23.5 6 21.9 26.474999999999998 26.650000000000002 24.975 7 23.775 26.174999999999997 27.625 22.425 8 18.85 22.925 32.300000000000004 25.924999999999997 9 22.625 22.525000000000002 31.724999999999998 23.125 10-14 23.105 25.900000000000002 26.685 24.310000000000002 15-19 24.115000000000002 24.69 25.85 25.345000000000002 20-24 23.68 25.275 26.224999999999998 24.82 25-29 23.785 24.560000000000002 26.44 25.215 30-34 24.115000000000002 25.619999999999997 25.46 24.805 35-39 24.435000000000002 25.69 25.480000000000004 24.395 40-44 24.41 25.795 24.779999999999998 25.014999999999997 45-49 25.055 25.259999999999998 24.995 24.69 50-54 24.282141070535268 26.038019009504755 25.007503751875937 24.67233616808404 55-59 24.185000000000002 24.81 25.295 25.71 60-64 24.97 24.175 25.590000000000003 25.264999999999997 65-69 24.375 25.480000000000004 25.485000000000003 24.66 70-74 24.044999999999998 24.965 26.07 24.92 75-79 24.43 25.009999999999998 25.795 24.765 80-84 24.69 25.979999999999997 24.6 24.73 85-89 24.235 26.325 24.995 24.445 90-94 24.68 25.974999999999998 25.330000000000002 24.015 95-99 25.145 25.145 25.180000000000003 24.529999999999998 100-104 24.705 25.53 24.69 25.074999999999996 105-109 24.15 25.374999999999996 25.36 25.115 110-114 24.59 25.019999999999996 25.419999999999998 24.97 115-119 24.83 25.619999999999997 25.085 24.465 120-124 24.585 25.174999999999997 25.25 24.990000000000002 125-129 25.095 25.035 25.915 23.955000000000002 130-134 24.455 24.97 25.955000000000002 24.62 135-139 23.9 25.369999999999997 25.755 24.975 140-144 24.77 26.44 25.34 23.45 145-149 25.0 27.455000000000002 24.18 23.365 150 26.525 27.400000000000002 23.799999999999997 22.275 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.5 3 1.0 4 1.0 5 1.5 6 1.0 7 1.0 8 1.0 9 0.0 10 0.5 11 1.5 12 1.5 13 1.5 14 2.0 15 2.0 16 3.5 17 4.0 18 3.0 19 4.5 20 3.0 21 1.5 22 3.0 23 3.0 24 3.0 25 4.5 26 9.5 27 8.5 28 6.0 29 11.5 30 16.0 31 18.0 32 23.0 33 24.0 34 24.0 35 36.0 36 52.5 37 61.5 38 65.0 39 77.0 40 100.0 41 128.5 42 149.5 43 164.5 44 187.0 45 204.0 46 184.0 47 172.5 48 161.5 49 154.0 50 178.0 51 172.0 52 159.5 53 149.0 54 129.5 55 121.0 56 120.5 57 113.5 58 104.5 59 107.0 60 107.0 61 84.0 62 60.0 63 53.5 64 43.0 65 32.5 66 30.5 67 30.5 68 24.5 69 17.0 70 14.5 71 12.0 72 7.5 73 6.5 74 5.0 75 4.0 76 7.5 77 8.5 78 6.5 79 2.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 19.475 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.05 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 150 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.95 #Duplication Level Percentage of deduplicated Percentage of total 1 91.32851584213452 82.15 2 6.725958866036687 12.1 3 1.6120066703724294 4.35 4 0.16675931072818231 0.6 5 0.11117287381878821 0.5 6 0.055586436909394105 0.3 7 0.0 0.0 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCCAAA 6 0.15 No Hit CCACTACTTTTAACGTTATTTTACTTATTCCGTGAATCGGAGGCGGGGCG 6 0.15 No Hit CTGTGATGCCCTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTAT 5 0.125 No Hit AAGGCCGCTTCGGCGGCCGATCCGGGCGGAAGACATTGTCAGGTGGGGAG 5 0.125 No Hit CGGAAGACATTGTCAGGTGGGGAGTTTGGCTGGGGCGGCACATCTGTTAA 5 0.125 No Hit CTATAGACTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACAT 5 0.125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.0 0.0 0.0 0.0 0.0 86-87 0.0 0.0 0.0 0.0 0.0 88-89 0.0 0.0 0.0 0.0 0.0 90-91 0.0 0.0 0.0 0.0 0.0 92-93 0.0 0.0 0.0 0.0 0.0 94-95 0.0 0.0 0.0 0.0 0.0 96-97 0.0 0.0 0.0 0.0 0.0 98-99 0.0 0.0 0.0 0.0 0.0 100-101 0.0 0.0 0.0 0.0 0.0 102-103 0.0 0.0 0.0 0.0 0.0 104-105 0.0 0.0 0.0 0.0 0.0 106-107 0.0 0.0 0.0 0.0 0.0 108-109 0.0 0.0 0.0 0.0 0.0 110-111 0.0 0.0 0.0 0.0 0.0 112-113 0.0 0.0 0.0 0.0 0.0 114-115 0.0 0.0 0.0 0.0 0.0 116-117 0.0 0.0 0.0 0.0 0.0 118-119 0.0 0.0 0.0 0.0 0.0 120-121 0.0 0.0 0.0 0.0 0.0 122-123 0.0 0.0 0.0 0.0 0.0 124-125 0.0 0.0 0.0 0.0 0.0 126-127 0.0 0.0 0.0 0.0 0.0 128-129 0.0 0.0 0.0 0.0 0.0 130-131 0.0 0.0 0.0 0.0 0.0 132-133 0.0 0.0 0.0 0.0 0.0 134-135 0.0 0.0 0.0 0.0 0.0 136-137 0.0 0.0 0.0 0.0 0.0 138 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAAATT 10 0.0070099696 143.75 9 >>END_MODULE Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181162 spots for SRR18274410.sra Written 1181162 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra Read 1181161 spots for SRR18274410.sra Written 1181161 spots for SRR18274410.sra SRR ids: ['SRR18274410.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_uy3kyig9 SRR18274410.sra spots: 23623221 blocks: [[1, 1181161], [1181162, 2362322], [2362323, 3543483], [3543484, 4724644], [4724645, 5905805], [5905806, 7086966], [7086967, 8268127], [8268128, 9449288], [9449289, 10630449], [10630450, 11811610], [11811611, 12992771], [12992772, 14173932], [14173933, 15355093], [15355094, 16536254], [16536255, 17717415], [17717416, 18898576], [18898577, 20079737], [20079738, 21260898], [21260899, 22442059], [22442060, 23623221]] SRR18274410 file size 8675887 SRR18274410 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR18274410 SRR18274410_1.fastq SRR18274410_2.fastq Input file: SRR18274410_1.fastq Paired file: SRR18274410_2.fastq trimmed: SRR18274410-trimmed-pair1.fastq, SRR18274410-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 03:51:30 2025 >> started Tue Feb 11 04:00:15 2025 >> done (524.226s) 23623221 read pairs processed; of these: 0 ( 0.00%) short read pairs filtered out after trimming by size control 0 ( 0.00%) empty read pairs filtered out after trimming by size control 23623221 (100.00%) read pairs available; of these: 3312421 (14.02%) trimmed read pairs available after processing 20310800 (85.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 25 1 0.00% 26 0 0.00% 27 0 0.00% 28 0 0.00% 29 0 0.00% 30 0 0.00% 31 0 0.00% 32 0 0.00% 33 0 0.00% 34 0 0.00% 35 0 0.00% 36 1 0.00% 37 1 0.00% 38 0 0.00% 39 0 0.00% 40 0 0.00% 41 0 0.00% 42 0 0.00% 43 0 0.00% 44 0 0.00% 45 0 0.00% 46 0 0.00% 47 0 0.00% 48 0 0.00% 49 0 0.00% 50 1 0.00% 51 0 0.00% 52 0 0.00% 53 0 0.00% 54 1 0.00% 55 0 0.00% 56 0 0.00% 57 0 0.00% 58 0 0.00% 59 0 0.00% 60 0 0.00% 61 0 0.00% 62 0 0.00% 63 0 0.00% 64 0 0.00% 65 1 0.00% 66 0 0.00% 67 0 0.00% 68 0 0.00% 69 1 0.00% 70 0 0.00% 71 0 0.00% 72 0 0.00% 73 0 0.00% 74 0 0.00% 75 0 0.00% 76 0 0.00% 77 0 0.00% 78 1 0.00% 79 0 0.00% 80 0 0.00% 81 0 0.00% 82 0 0.00% 83 1 0.00% 84 0 0.00% 85 0 0.00% 86 0 0.00% 87 1 0.00% 88 1 0.00% 89 0 0.00% 90 0 0.00% 91 0 0.00% 92 0 0.00% 93 1 0.00% 94 0 0.00% 95 0 0.00% 96 1 0.00% 97 0 0.00% 98 0 0.00% 99 2 0.00% 100 0 0.00% 101 2 0.00% 102 0 0.00% 103 0 0.00% 104 0 0.00% 105 0 0.00% 106 0 0.00% 107 0 0.00% 108 1 0.00% 109 0 0.00% 110 1 0.00% 111 1 0.00% 112 1 0.00% 113 1 0.00% 114 0 0.00% 115 0 0.00% 116 0 0.00% 117 0 0.00% 118 1 0.00% 119 0 0.00% 120 1 0.00% 121 1 0.00% 122 2 0.00% 123 7 0.00% 124 0 0.00% 125 6 0.00% 126 2 0.00% 127 2 0.00% 128 6 0.00% 129 4 0.00% 130 6 0.00% 131 7 0.00% 132 2 0.00% 133 0 0.00% 134 0 0.00% 135 0 0.00% 136 3 0.00% 137 4 0.00% 138 14 0.00% 139 155 0.00% 140 3057 0.01% 141 299325 1.27% 142 299678 1.27% 143 301773 1.28% 144 298347 1.26% 145 304888 1.29% 146 303501 1.28% 147 311608 1.32% 148 347849 1.47% 149 842150 3.56% 150 20310800 85.98% 23623221 reads passed initial QC criterion=sequence-density sequence-density=0.88 sequence-density-rank=1 fanout-score=1.26 fanout-score-rank=37 prefix-density=0.21 prefix-fanout=1.3 sequence=TTTGTGTTTGAG criterion=fanout-score sequence-density=0.02 sequence-density-rank=39 fanout-score=161.97 fanout-score-rank=1 prefix-density=1.94 prefix-fanout=1.5 sequence=CGCATCGCCGGCCCCCATCCACTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCAGACAGCGCCTCGTGGTGCGGCAGGGTCCAGCCACGACGGGGCTCTCACCCTCTCCGGCGCCCCTTTCCAGGGGACTTGGGCCTGGTCCGCCGCTGAGGACGCTTCTCCAGACTACAATTCGGACGCCGCAGGCGCCAGATTCTCAAGCTGGGCATTTCCCGGTTCGCTCGCCGTTACTAGGGGAATCCTTGTAAGTTTCTTTTCCTCCGCTT criterion=sequence-density sequence-density=1.78 sequence-density-rank=1 fanout-score=2.20 fanout-score-rank=37 prefix-density=2.13 prefix-fanout=1.8 sequence=TTTGTGTTTGAG criterion=fanout-score sequence-density=0.03 sequence-density-rank=44 fanout-score=111.37 fanout-score-rank=1 prefix-density=1.86 prefix-fanout=1.5 sequence=CGCATCGCCGGCCCCCATCCACTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCAGACAGCGCCTCGTGGTGCGGCAGGGTCCAGCCACGACGGGGCTCTCACCCTCTCCGGCGCCCCTTTCCAGGGGACTTGGGCCTGGTCCGCCGCTGAGGACGCTTCTCCAGACTACAATTCGGACGCCGCAGGCGCCAGATTCTCAAGCTGGGCATTTCCCGGTTCGCTCGCCGTTACTAGGGGAATCCTTGTAAGTTTCTTTTCCTCCGCTT SRR18274410 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 04:26:35 Started mapping on | Feb 11 04:26:51 Finished on | Feb 11 05:48:12 Mapping speed, Million of reads per hour | 17.42 Number of input reads | 23623221 Average input read length | 290 UNIQUE READS: Uniquely mapped reads number | 6488997 Uniquely mapped reads % | 27.47% Average mapped length | 279.91 Number of splices: Total | 3336132 Number of splices: Annotated (sjdb) | 3222054 Number of splices: GT/AG | 3243388 Number of splices: GC/AG | 45215 Number of splices: AT/AC | 4322 Number of splices: Non-canonical | 43207 Mismatch rate per base, % | 0.69% Deletion rate per base | 0.01% Deletion average length | 2.21 Insertion rate per base | 0.03% Insertion average length | 3.81 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 574868 % of reads mapped to multiple loci | 2.43% Number of reads mapped to too many loci | 10849485 % of reads mapped to too many loci | 45.93% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 13.34% % of reads unmapped: other | 10.83% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 16559356 16559356 16559356 N_multimapping 574868 574868 574868 N_noFeature 2109585 4296692 4234832 N_ambiguous 111281 22334 22063 UnstrandedReadsAssigned:4268131 PositiveStrandReadsAssigned:2169971 NegativeStrandReadsAssigned:2232102 Dataset is classified unstranded MeadianReadLen=150 20thPercentileLength=150 echo kmer=145 SRR18274410 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR18274410-trimmed-pair1.fastq SRR18274410-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 23,623,221 reads, 18,761,076 reads pseudoaligned [quant] estimated average fragment length: 177.714 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,228 rounds 52401 SRR18274410.ke.tsv 34699 SRR18274410.se.tsv 87100 total ==> SRR18274410.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1841.29 320 5.62682 Potri.005G024800.1.v4.1 1035 858.286 127 4.79077 Potri.004G059700.1.v4.1 961 784.286 26 1.07333 Potri.007G009000.2.v4.1 1416 1239.29 0 0 Potri.003G141000.2.v4.1 2943 2766.29 92.1791 1.07887 Potri.016G087400.1.v4.1 270 99.2505 228 74.3765 Potri.015G069301.1.v4.1 564 387.364 0 0 Potri.010G195200.1.v4.1 1773 1596.29 0 0 Potri.012G127500.1.v4.1 977 800.286 43 1.73963 ==> SRR18274410.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 216 Potri.001G233950.v4.1 1 Potri.001G122700.v4.1 127 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 26 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR18274410 completed mapping pipeline successfully