Starting /dee2/code/volunteer_pipeline.sh SRR22215328 current disk space = 3055677104128 free memory = 1478667400 SRR22215328 SRAfilesize edf150c516658c2964be34db2bb2e071 SRR22215328.sra SRR22215328.sra file validated SRR22215328 is paired end SRR22215328 is conventional basespace SRR22215328 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR22215328_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.82325 37.0 37.0 37.0 37.0 37.0 2 35.96775 37.0 37.0 37.0 37.0 37.0 3 36.234 37.0 37.0 37.0 37.0 37.0 4 36.1645 37.0 37.0 37.0 37.0 37.0 5 36.306 37.0 37.0 37.0 37.0 37.0 6 36.2595 37.0 37.0 37.0 37.0 37.0 7 36.213 37.0 37.0 37.0 37.0 37.0 8 36.302 37.0 37.0 37.0 37.0 37.0 9 36.2455 37.0 37.0 37.0 37.0 37.0 10-14 36.2483 37.0 37.0 37.0 37.0 37.0 15-19 36.199 37.0 37.0 37.0 37.0 37.0 20-24 36.20270000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.0588 37.0 37.0 37.0 37.0 37.0 30-34 36.0265 37.0 37.0 37.0 37.0 37.0 35-39 36.0107 37.0 37.0 37.0 37.0 37.0 40-44 35.86899999999999 37.0 37.0 37.0 37.0 37.0 45-49 35.8212 37.0 37.0 37.0 37.0 37.0 50-54 35.6536 37.0 37.0 37.0 37.0 37.0 55-59 35.620900000000006 37.0 37.0 37.0 37.0 37.0 60-64 35.5607 37.0 37.0 37.0 37.0 37.0 65-69 35.53830000000001 37.0 37.0 37.0 37.0 37.0 70-74 35.56 37.0 37.0 37.0 37.0 37.0 75-79 35.6472 37.0 37.0 37.0 37.0 37.0 80-84 35.5266 37.0 37.0 37.0 37.0 37.0 85-89 35.5278 37.0 37.0 37.0 37.0 37.0 90-94 35.4095 37.0 37.0 37.0 37.0 37.0 95-99 35.421600000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.3054 37.0 37.0 37.0 32.2 37.0 105-109 35.2875 37.0 37.0 37.0 34.6 37.0 110-114 35.2299 37.0 37.0 37.0 34.6 37.0 115-119 35.227999999999994 37.0 37.0 37.0 32.2 37.0 120-124 35.064499999999995 37.0 37.0 37.0 25.0 37.0 125-129 35.1402 37.0 37.0 37.0 27.4 37.0 130-134 35.0339 37.0 37.0 37.0 25.0 37.0 135-139 34.987899999999996 37.0 37.0 37.0 25.0 37.0 140-144 34.8091 37.0 37.0 37.0 25.0 37.0 145-149 34.7168 37.0 37.0 37.0 25.0 37.0 150-151 34.456500000000005 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 0.0 22 0.0 23 5.0 24 5.0 25 12.0 26 10.0 27 30.0 28 32.0 29 51.0 30 73.0 31 73.0 32 123.0 33 160.0 34 248.0 35 582.0 36 2447.0 37 147.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.87383764765016 11.108318673033425 14.903242020608193 36.11460165870822 2 31.68044077134986 12.972702228900577 27.097420485850236 28.249436513899322 3 29.575000000000003 18.0 21.0 31.424999999999997 4 30.725 22.85 18.75 27.675 5 28.875 28.025 20.974999999999998 22.125 6 21.8 28.925 24.675 24.6 7 16.0 27.925 35.225 20.849999999999998 8 17.2 25.674999999999997 29.9 27.224999999999998 9 21.0 20.025000000000002 32.125 26.85 10-14 20.965 28.93 25.124999999999996 24.98 15-19 22.405 25.985000000000003 26.13 25.480000000000004 20-24 21.654999999999998 27.41 25.240000000000002 25.695 25-29 22.009999999999998 26.27 25.405 26.314999999999998 30-34 21.060000000000002 25.69 26.215 27.034999999999997 35-39 21.57 27.205000000000002 24.395 26.83 40-44 22.365 25.47 25.295 26.87 45-49 22.795 25.064999999999998 25.905 26.235000000000003 50-54 21.575 25.869999999999997 25.590000000000003 26.965 55-59 21.85 27.045 24.87 26.235000000000003 60-64 21.88 26.669999999999998 25.945 25.505 65-69 21.310000000000002 27.01 25.335 26.345000000000002 70-74 22.814999999999998 26.61 24.485 26.090000000000003 75-79 21.995 26.275 24.834999999999997 26.895000000000003 80-84 22.505 25.564999999999998 24.595 27.334999999999997 85-89 23.244999999999997 24.965 24.675 27.115000000000002 90-94 22.884999999999998 25.455 24.63 27.029999999999998 95-99 22.805 25.365 25.4 26.43 100-104 22.509999999999998 25.790000000000003 25.435000000000002 26.265 105-109 22.425 26.27 24.95 26.355 110-114 22.53 25.81 25.15 26.51 115-119 22.965 25.88 24.89 26.265 120-124 22.869999999999997 26.525 24.915000000000003 25.69 125-129 23.724999999999998 25.430000000000003 23.9 26.945000000000004 130-134 23.225 25.96 24.665 26.150000000000002 135-139 22.895 26.41 24.075 26.619999999999997 140-144 22.8 26.855 24.265 26.08 145-149 23.22 27.1 24.635 25.045 150-151 22.900000000000002 27.900000000000002 23.575 25.624999999999996 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 1.5 17 2.0 18 2.0 19 1.5 20 2.0 21 2.5 22 3.5 23 3.0 24 4.0 25 5.5 26 5.0 27 9.5 28 15.0 29 24.0 30 27.5 31 25.0 32 31.5 33 35.0 34 45.5 35 64.0 36 79.0 37 80.5 38 77.0 39 98.5 40 124.0 41 137.0 42 134.5 43 143.5 44 162.0 45 165.5 46 163.5 47 184.0 48 182.0 49 169.5 50 167.0 51 143.0 52 124.0 53 119.0 54 103.0 55 90.0 56 89.0 57 75.5 58 84.0 59 90.5 60 76.5 61 72.0 62 73.0 63 59.5 64 40.0 65 23.5 66 24.0 67 28.0 68 27.0 69 34.5 70 31.0 71 23.0 72 27.0 73 35.5 74 37.5 75 32.0 76 26.5 77 15.5 78 6.5 79 4.5 80 2.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.525 2 0.17500000000000002 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.475 #Duplication Level Percentage of deduplicated Percentage of total 1 86.45043952712943 71.3 2 9.821157926644437 16.2 3 2.1218551076083663 5.25 4 0.7881176113973931 2.6 5 0.33343437405274323 1.375 6 0.24249772658381327 1.2 7 0.03031221582297666 0.17500000000000002 8 0.06062443164595332 0.4 9 0.03031221582297666 0.22499999999999998 >10 0.12124886329190664 1.275 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGGTTGG 14 0.35000000000000003 TruSeq Adapter, Index 1 (97% over 45bp) CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA 14 0.35000000000000003 No Hit CGGCAATCGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGACT 13 0.325 No Hit GTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATC 10 0.25 No Hit CGATAGAACTCGCACCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAGGG 9 0.22499999999999998 No Hit GGTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTA 8 0.2 No Hit GTCGGATTCCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCG 8 0.2 No Hit GTCGGCAATCGGACGGCGGGCGCACGCGTCGCATCTAGCCCGGATTCTGA 7 0.17500000000000002 No Hit GAGGGAACCAGCTACTAGACGGTTCGATTAGTCTTTCGCCCCTATACCCA 6 0.15 No Hit GGGAATTGTACTTCAAGGCGGCCCGCGCGGCTCTTTCACCGCGAGGGCTT 6 0.15 No Hit GCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGC 6 0.15 No Hit GCCGGGGCACGCCGTGACGGCGCGCGCTGCAGTCCACGATCGCGACGACG 6 0.15 No Hit GCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCCTC 6 0.15 No Hit GTGGTATTTCACCTTCGCCGAAGCTCCCACTTATCCTACACCTCTCAAGT 6 0.15 No Hit CCCACCTGACAATGTCTTCCGCCCGGATCGGCCGCCGAAGCGGCCTTGGG 6 0.15 No Hit CGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAGCC 6 0.15 No Hit GCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTGT 5 0.125 No Hit GGGGAGCCCACAGGCCGATGCCCGGAGCGCGCATGTGCCGGGGCACGCCG 5 0.125 No Hit GGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGC 5 0.125 No Hit CTCGTTGAATACATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGG 5 0.125 No Hit GGGACGAATGGGGAGCCCACAGGCCGATGCCCGGAGCGCGCATGTGCCGG 5 0.125 No Hit GCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGC 5 0.125 No Hit CACAGGCCGATGCCCGGAGCGCGCATGTGCCGGGGCACGCCGTGACGGCG 5 0.125 No Hit GCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGC 5 0.125 No Hit CCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGG 5 0.125 No Hit CCCGACTCGCAGACAGCGCCTCGTGGTGCGGCAGGGTCCAGCCACGACGG 5 0.125 No Hit CGGGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.037500000000000006 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.075 0.0 0.0 0.0 0.0 88-89 0.075 0.0 0.0 0.0 0.0 90-91 0.075 0.0 0.0 0.0 0.0 92-93 0.1 0.0 0.0 0.0 0.0 94-95 0.1 0.0 0.0 0.0 0.0 96-97 0.1 0.0 0.0 0.0 0.0 98-99 0.1 0.0 0.0 0.0 0.0 100-101 0.125 0.0 0.0 0.0 0.0 102-103 0.2 0.0 0.0 0.0 0.0 104-105 0.25 0.0 0.0 0.0 0.0 106-107 0.3125 0.0 0.0 0.0 0.0 108-109 0.4 0.0 0.0 0.0 0.0 110-111 0.475 0.0 0.0 0.0 0.0 112-113 0.55 0.0 0.0 0.0 0.0 114-115 0.625 0.0 0.0 0.0 0.0 116-117 0.7625 0.0 0.0 0.0 0.0 118-119 0.8625 0.0 0.0 0.0 0.0 120-121 0.95 0.0 0.0 0.0 0.0 122-123 1.1125 0.0 0.0 0.0 0.0 124-125 1.5125 0.0 0.0 0.0 0.0 126-127 1.7000000000000002 0.0 0.0 0.0 0.0 128-129 1.9875 0.0 0.0 0.0 0.0 130-131 2.3875 0.0 0.0 0.0 0.0 132-133 3.05 0.0 0.0 0.0 0.0 134-135 3.6125 0.0 0.0 0.0 0.0 136-137 4.3 0.0 0.0 0.0 0.0 138-139 5.0625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATACTG 10 0.006830828 145.0 7 CAATCGG 10 0.006830828 145.0 6 GCAATCG 10 0.006830828 145.0 5 ATCGGAC 10 0.006830828 145.0 8 AATCGGA 10 0.006830828 145.0 7 >>END_MODULE SRR22215328 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR22215328_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.608 37.0 37.0 37.0 37.0 37.0 2 35.988 37.0 37.0 37.0 37.0 37.0 3 36.044 37.0 37.0 37.0 37.0 37.0 4 36.05 37.0 37.0 37.0 37.0 37.0 5 35.977 37.0 37.0 37.0 37.0 37.0 6 36.0265 37.0 37.0 37.0 37.0 37.0 7 36.0695 37.0 37.0 37.0 37.0 37.0 8 35.978 37.0 37.0 37.0 37.0 37.0 9 35.951 37.0 37.0 37.0 37.0 37.0 10-14 35.9298 37.0 37.0 37.0 37.0 37.0 15-19 35.8925 37.0 37.0 37.0 37.0 37.0 20-24 35.8568 37.0 37.0 37.0 37.0 37.0 25-29 35.705600000000004 37.0 37.0 37.0 37.0 37.0 30-34 35.68770000000001 37.0 37.0 37.0 37.0 37.0 35-39 35.644600000000004 37.0 37.0 37.0 37.0 37.0 40-44 35.6025 37.0 37.0 37.0 37.0 37.0 45-49 35.6421 37.0 37.0 37.0 37.0 37.0 50-54 35.6263 37.0 37.0 37.0 37.0 37.0 55-59 35.515 37.0 37.0 37.0 37.0 37.0 60-64 35.4512 37.0 37.0 37.0 37.0 37.0 65-69 35.4756 37.0 37.0 37.0 37.0 37.0 70-74 35.4434 37.0 37.0 37.0 37.0 37.0 75-79 35.4542 37.0 37.0 37.0 37.0 37.0 80-84 35.3963 37.0 37.0 37.0 37.0 37.0 85-89 35.4337 37.0 37.0 37.0 37.0 37.0 90-94 35.4276 37.0 37.0 37.0 34.6 37.0 95-99 35.4592 37.0 37.0 37.0 37.0 37.0 100-104 35.364 37.0 37.0 37.0 34.6 37.0 105-109 35.3684 37.0 37.0 37.0 37.0 37.0 110-114 35.4382 37.0 37.0 37.0 37.0 37.0 115-119 35.311099999999996 37.0 37.0 37.0 32.2 37.0 120-124 35.2923 37.0 37.0 37.0 32.2 37.0 125-129 35.193799999999996 37.0 37.0 37.0 29.8 37.0 130-134 35.1678 37.0 37.0 37.0 29.8 37.0 135-139 35.0951 37.0 37.0 37.0 25.0 37.0 140-144 35.0189 37.0 37.0 37.0 25.0 37.0 145-149 34.92360000000001 37.0 37.0 37.0 25.0 37.0 150-151 34.913250000000005 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 2.0 15 1.0 16 2.0 17 3.0 18 0.0 19 1.0 20 5.0 21 5.0 22 9.0 23 8.0 24 14.0 25 19.0 26 23.0 27 26.0 28 22.0 29 41.0 30 37.0 31 65.0 32 74.0 33 138.0 34 265.0 35 714.0 36 2340.0 37 186.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.425 20.025000000000002 15.575 31.974999999999998 2 31.35 25.25 26.6 16.8 3 24.575 30.3 26.8 18.325 4 28.1 34.050000000000004 20.0 17.849999999999998 5 30.2 33.900000000000006 20.25 15.65 6 23.0 37.875 21.15 17.974999999999998 7 24.325 18.55 35.3 21.825 8 25.7 22.275 27.025 25.0 9 26.950000000000003 22.275 28.275 22.5 10-14 27.450000000000003 26.240000000000002 24.755 21.555 15-19 27.985 25.240000000000002 25.650000000000002 21.125 20-24 27.46 26.205000000000002 25.39 20.945 25-29 26.979999999999997 26.075 25.94 21.005 30-34 27.150000000000002 25.64 25.615 21.595 35-39 26.205000000000002 25.85 26.13 21.815 40-44 26.224999999999998 25.85 26.650000000000002 21.275 45-49 26.8 25.669999999999998 25.91 21.62 50-54 28.03 25.324999999999996 25.095 21.55 55-59 27.01 25.575 26.135 21.279999999999998 60-64 27.21 25.759999999999998 26.125 20.905 65-69 26.75 26.340000000000003 25.619999999999997 21.29 70-74 27.54 25.34 25.545 21.575 75-79 28.005000000000003 24.54 25.56 21.895 80-84 27.58 24.87 26.295 21.255 85-89 27.155 25.814999999999998 25.324999999999996 21.705 90-94 27.325 25.655 25.055 21.965 95-99 26.775 26.525 25.419999999999998 21.279999999999998 100-104 27.3 25.645 25.619999999999997 21.435000000000002 105-109 27.325 25.305 25.775 21.595 110-114 27.345000000000002 25.974999999999998 25.365 21.315 115-119 27.22 25.595000000000002 25.790000000000003 21.395 120-124 27.815 25.119999999999997 25.61 21.455 125-129 27.339999999999996 25.540000000000003 25.505 21.615000000000002 130-134 27.950000000000003 25.69 25.03 21.33 135-139 27.779999999999998 25.665 25.155 21.4 140-144 27.389999999999997 26.265 24.955 21.39 145-149 28.305000000000003 25.990000000000002 24.65 21.055 150-151 29.025000000000002 26.9125 24.15 19.9125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.5 6 0.5 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 2.0 17 2.0 18 2.5 19 2.0 20 2.0 21 2.0 22 2.0 23 4.0 24 3.0 25 3.5 26 6.0 27 6.5 28 7.5 29 9.0 30 14.5 31 21.5 32 24.5 33 32.0 34 44.5 35 59.5 36 72.0 37 74.5 38 84.0 39 102.0 40 119.0 41 130.5 42 152.0 43 176.0 44 191.5 45 209.0 46 224.0 47 211.5 48 192.0 49 184.5 50 161.0 51 133.5 52 122.0 53 123.0 54 115.5 55 98.5 56 82.5 57 75.5 58 68.0 59 57.0 60 57.0 61 56.0 62 52.0 63 51.5 64 38.5 65 20.5 66 15.5 67 21.0 68 23.0 69 24.0 70 24.0 71 18.5 72 25.5 73 33.5 74 28.5 75 23.0 76 13.5 77 11.5 78 12.0 79 5.0 80 2.0 81 1.5 82 2.5 83 2.5 84 3.0 85 2.5 86 2.0 87 2.0 88 1.0 89 1.0 90 1.5 91 1.5 92 0.5 93 1.0 94 2.5 95 3.0 96 1.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.825 #Duplication Level Percentage of deduplicated Percentage of total 1 85.76229758631226 70.175 2 9.929728078215705 16.25 3 2.444240757714635 6.0 4 1.0388023220287197 3.4000000000000004 5 0.4277421326000611 1.7500000000000002 6 0.15276504735716467 0.75 7 0.09165902841429881 0.525 8 0.061106018942865874 0.4 9 0.030553009471432937 0.22499999999999998 >10 0.061106018942865874 0.525 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGAAAATGGATGGCGCTGAAGCGCGCGACCTATACCCGGCCGTCGGGGCA 11 0.27499999999999997 No Hit GTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGACAGTGTC 10 0.25 No Hit AGAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGA 9 0.22499999999999998 No Hit CGATCGGCTCGGGGCGTGGACCGATGCGGATCGCGGTGGCGGCCCAAGCC 8 0.2 No Hit ATTGGCTCTGAGGGCTGGGCTCGGGGGTCCCAGTCCCGAACCCGTCGGCT 8 0.2 No Hit GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAAC 7 0.17500000000000002 No Hit GCTCGTTTGATTCTGATTTCCAGTACGAATACGAACCGTGAAAGCGTGGC 7 0.17500000000000002 No Hit GTTAGTTTTACCCTACTGATGACAGTGTCGCAATAGTAATCCAACCTAGT 7 0.17500000000000002 No Hit TAAGGATTGACAGACTGAGAGCTCTTTCTTGATTCTATGGGTGGTGGTGC 6 0.15 No Hit GAAACTTACAAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAAATGCCC 6 0.15 No Hit CGCCGACCGACCTTGATCTTCTGAGAAGGGTTCGAGTGAGAGCATGCCTG 6 0.15 No Hit AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCAGCCAAGCGTTCATAG 6 0.15 No Hit GGAACGGTTACAGCCGGTCCGCCGATCGGCTCGGGGCGTGGACCGATGCG 6 0.15 No Hit CTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTT 5 0.125 No Hit CAAGTCTGGTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATT 5 0.125 No Hit GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAAC 5 0.125 No Hit CTTAGATGTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTC 5 0.125 No Hit CTCAGGATAGCTGGAGCTCGGTGCGAGTTCTATCGGGTAAAGCCAATGAT 5 0.125 No Hit GGATAAGTGGGAGCTTCGGCGAAGGTGAAATACCACTACTTTTAACGTTA 5 0.125 No Hit CTCAAACTTTAAATAGGTAGGACGGCGCGGCTGCTTCGTTGAGCCGCGCC 5 0.125 No Hit CCCGAACCCGTCGGCTGTCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAG 5 0.125 No Hit GAAGACCCTGTTGAGCTTGACTCTAGTCCGACTTTGTGAAATGACTTGAG 5 0.125 No Hit CGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAA 5 0.125 No Hit AGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAA 5 0.125 No Hit TGGTGAACTATGCCTGAGCGGGGCGAAGCCAGAGGAAACTCTGGTGGAGG 5 0.125 No Hit CTTGACTCTAGTCCGACTTTGTGAAATGACTTGAGAGGTGTAGGATAAGT 5 0.125 No Hit CCGGAAGCCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.037500000000000006 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.075 0.0 0.0 0.0 0.0 88-89 0.075 0.0 0.0 0.0 0.0 90-91 0.075 0.0 0.0 0.0 0.0 92-93 0.1 0.0 0.0 0.0 0.0 94-95 0.1 0.0 0.0 0.0 0.0 96-97 0.1 0.0 0.0 0.0 0.0 98-99 0.1 0.0 0.0 0.0 0.0 100-101 0.125 0.0 0.0 0.0 0.0 102-103 0.2 0.0 0.0 0.0 0.0 104-105 0.25 0.0 0.0 0.0 0.0 106-107 0.3125 0.0 0.0 0.0 0.0 108-109 0.4 0.0 0.0 0.0 0.0 110-111 0.475 0.0 0.0 0.0 0.0 112-113 0.55 0.0 0.0 0.0 0.0 114-115 0.625 0.0 0.0 0.0 0.0 116-117 0.7625 0.0 0.0 0.0 0.0 118-119 0.8625 0.0 0.0 0.0 0.0 120-121 1.0 0.0 0.0 0.0 0.0 122-123 1.1625 0.0 0.0 0.0 0.0 124-125 1.5750000000000002 0.0 0.0 0.0 0.0 126-127 1.7125 0.0 0.0 0.0 0.0 128-129 1.9875 0.0 0.0 0.0 0.0 130-131 2.4125 0.0 0.0 0.0 0.0 132-133 3.125 0.0 0.0 0.0 0.0 134-135 3.675 0.0 0.0 0.0 0.0 136-137 4.375 0.0 0.0 0.0 0.0 138-139 5.1125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGTTGC 10 0.006830828 145.0 9 CAACTTG 15 1.1411342E-4 145.0 1 AACTTGA 10 0.006830828 145.0 2 >>END_MODULE Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972739 spots for SRR22215328.sra Written 1972739 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra Read 1972725 spots for SRR22215328.sra Written 1972725 spots for SRR22215328.sra SRR ids: ['SRR22215328.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_5bp6pvp_ SRR22215328.sra spots: 39454514 blocks: [[1, 1972725], [1972726, 3945450], [3945451, 5918175], [5918176, 7890900], [7890901, 9863625], [9863626, 11836350], [11836351, 13809075], [13809076, 15781800], [15781801, 17754525], [17754526, 19727250], [19727251, 21699975], [21699976, 23672700], [23672701, 25645425], [25645426, 27618150], [27618151, 29590875], [29590876, 31563600], [31563601, 33536325], [33536326, 35509050], [35509051, 37481775], [37481776, 39454514]] SRR22215328 file size 13386669 SRR22215328 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR22215328 SRR22215328_1.fastq SRR22215328_2.fastq Input file: SRR22215328_1.fastq Paired file: SRR22215328_2.fastq trimmed: SRR22215328-trimmed-pair1.fastq, SRR22215328-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 07:41:54 2025 >> started Tue Feb 11 07:42:38 2025 >> done (44.283s) 39454514 read pairs processed; of these: 454 ( 0.00%) short read pairs filtered out after trimming by size control 278948 ( 0.71%) empty read pairs filtered out after trimming by size control 39175112 (99.29%) read pairs available; of these: 5110469 (13.05%) trimmed read pairs available after processing 34064643 (86.95%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 17 0.00% 19 10 0.00% 20 23 0.00% 21 15 0.00% 22 6 0.00% 23 6 0.00% 24 8 0.00% 25 8 0.00% 26 15 0.00% 27 28 0.00% 28 10 0.00% 29 87 0.00% 30 16 0.00% 31 16 0.00% 32 24 0.00% 33 20 0.00% 34 20 0.00% 35 24 0.00% 36 19 0.00% 37 19 0.00% 38 25 0.00% 39 24 0.00% 40 21 0.00% 41 15 0.00% 42 23 0.00% 43 25 0.00% 44 35 0.00% 45 38 0.00% 46 40 0.00% 47 46 0.00% 48 46 0.00% 49 49 0.00% 50 57 0.00% 51 85 0.00% 52 59 0.00% 53 75 0.00% 54 86 0.00% 55 89 0.00% 56 89 0.00% 57 77 0.00% 58 88 0.00% 59 91 0.00% 60 101 0.00% 61 116 0.00% 62 123 0.00% 63 119 0.00% 64 122 0.00% 65 136 0.00% 66 149 0.00% 67 177 0.00% 68 189 0.00% 69 198 0.00% 70 217 0.00% 71 274 0.00% 72 347 0.00% 73 375 0.00% 74 397 0.00% 75 429 0.00% 76 482 0.00% 77 532 0.00% 78 594 0.00% 79 658 0.00% 80 670 0.00% 81 739 0.00% 82 796 0.00% 83 886 0.00% 84 1029 0.00% 85 1193 0.00% 86 1229 0.00% 87 1373 0.00% 88 1529 0.00% 89 1638 0.00% 90 1810 0.00% 91 2073 0.01% 92 2398 0.01% 93 2526 0.01% 94 2806 0.01% 95 3221 0.01% 96 3565 0.01% 97 3898 0.01% 98 4294 0.01% 99 4649 0.01% 100 4723 0.01% 101 5211 0.01% 102 5967 0.02% 103 6259 0.02% 104 6894 0.02% 105 7540 0.02% 106 8001 0.02% 107 9054 0.02% 108 10054 0.03% 109 11808 0.03% 110 12274 0.03% 111 14122 0.04% 112 15732 0.04% 113 16977 0.04% 114 19180 0.05% 115 21891 0.06% 116 23995 0.06% 117 26058 0.07% 118 29338 0.07% 119 32808 0.08% 120 37572 0.10% 121 42469 0.11% 122 46994 0.12% 123 51137 0.13% 124 57675 0.15% 125 64408 0.16% 126 69580 0.18% 127 76090 0.19% 128 89423 0.23% 129 92572 0.24% 130 100803 0.26% 131 110223 0.28% 132 121187 0.31% 133 126273 0.32% 134 130779 0.33% 135 144596 0.37% 136 155602 0.40% 137 168255 0.43% 138 188826 0.48% 139 192587 0.49% 140 199492 0.51% 141 205262 0.52% 142 217304 0.55% 143 230237 0.59% 144 239935 0.61% 145 250153 0.64% 146 252912 0.65% 147 265420 0.68% 148 279502 0.71% 149 281278 0.72% 150 284406 0.73% 151 34064643 86.95% 39175112 reads passed initial QC criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=1.68 fanout-score-rank=36 prefix-density=0.62 prefix-fanout=1.0 sequence=CGACGGTCTAAACCCAGCTCACGTTCCCTATTGGTGGGTGAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACTTTTCTGACACCTCTAGCTTCAAATTCCGAAGGTCTAAAGGATCGATAGGCCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCGTTATCTTTTAACAGATGTGCCGCCCCAGCCAAACTCCCCACCTGACAATGTCTTCCGCCCGGATCGGCCGCCGAAGCGGCCTTGGGTCCAAAAAGAGGGGCAGCGCCCCGCCTCCGATTCACGGAATAAGTAAAATAACGTTAAAAGTAGTGGTATTTCACCTTCGCCGAAGCTCCCACTTATCCTACAC criterion=fanout-score sequence-density=0.24 sequence-density-rank=2 fanout-score=26.70 fanout-score-rank=1 prefix-density=3.04 prefix-fanout=2.1 sequence=CGCATCGCCGGCCCCCATCCACTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCAGACAGCGCCTCGTGGTGCGGCAGGGTCCAGCCACGACGGGGCTCTCACCCTCTCCGGCGCCCCTTTCCAGGGGACTTGGGCCTGGTCCGCCGCTGAGGACGCTTCTCCAGACTACAATTCGGACGCCGCAGGCGCCAGATTCTCAAGCTGGGCATTTCCCGGTTCGCTCGCCGTTACTAGGGGAATCCTTGTAAGTTTCTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTAGTCCCGCCTGACCTGGGGTCGCAACGAGGGCATCCTAGAAGGTCGATGCCCGAGGGTCCGGGAGATCCCGGGGGCGACGGGCGCGCGCACG criterion=sequence-density sequence-density=1.22 sequence-density-rank=1 fanout-score=2.38 fanout-score-rank=23 prefix-density=1.01 prefix-fanout=2.4 sequence=TTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGACAGTGTCGCAATAGTAATCCAACCTAGTACGAGAGGAACCGTTGATTCGCACAATTGGTCATCGCGCTTGGTTGAAAAGCCAGTGGCGCGAAGCTACCGTGCGTTGGATTATGACTGAACGCCTCTAAGTCAGAATCCGGGCTAGATGCGACGCGTGCGCCCGCCGTCCGATTGCCGACCTGCAGTAGGGGCCTCTTGGCCCCGGAGGCACGTGCCGTTGGCCAAGCCCTCGCGGTGAAAGAGCCGCGCGGGCCGCCTTGAAGTACAATTCCCACCGAGCGGCGGGTAGAATCCTTTGCAGACGACTTAAATACGCGACGGGGTATTGTAAGTGGCAGAGTGGCCTTGCTGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=30 fanout-score=49.24 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=1.0 sequence=CGCCCGTCGCCCCCGGGATCTCCCGGACCCTCGGGCATCGACCTTCTAGGATGCCCTCGTTGCGACCCCAGGTCAGGCGGGACTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCCCTAGTAACGGCGAGCGAACCGGGAAATGCCCAGCTTGAGAATCTGGCGCCTGCGGCGTCCGAATTGTAGTCTGGAGAAGCGTCCTCAGCGGCGGACCAGGCCCAAGTCCCCTGGAAAGGGGCGCCGGAGAGGGTGAGAGCCCCGTCGTGGCTGGACCCTGCCGCACCACGAGGCGCTGTCTGCGAGTCGGGTTGTTTGGGAATGCAGCCCCAATCGGGCGGTAAATTCCGTCCAAGGCTAAATACGGGCGAGAGACCGATAGCAAACAAGTACCGCGAGGGAAAGATGAAAAGGACTTTGAAAAGAGAGTCAAAGAGTGCTTGAAATTGTCGGGAGGGAAGTGGATGGGGGCCGGCGATGCG SRR22215328 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 07:43:27 Started mapping on | Feb 11 07:43:27 Finished on | Feb 11 07:52:19 Mapping speed, Million of reads per hour | 265.09 Number of input reads | 39175112 Average input read length | 298 UNIQUE READS: Uniquely mapped reads number | 22258624 Uniquely mapped reads % | 56.82% Average mapped length | 297.74 Number of splices: Total | 14881711 Number of splices: Annotated (sjdb) | 14537473 Number of splices: GT/AG | 14653688 Number of splices: GC/AG | 176318 Number of splices: AT/AC | 16702 Number of splices: Non-canonical | 35003 Mismatch rate per base, % | 0.34% Deletion rate per base | 0.03% Deletion average length | 2.20 Insertion rate per base | 0.02% Insertion average length | 1.79 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1360553 % of reads mapped to multiple loci | 3.47% Number of reads mapped to too many loci | 11518342 % of reads mapped to too many loci | 29.40% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.31% % of reads unmapped: other | 7.00% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 15555935 15555935 15555935 N_multimapping 1360553 1360553 1360553 N_noFeature 1166811 21792722 1327134 N_ambiguous 410890 3095 103032 UnstrandedReadsAssigned:20680923 PositiveStrandReadsAssigned:462807 NegativeStrandReadsAssigned:20828458 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR22215328 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR22215328-trimmed-pair1.fastq SRR22215328-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 39,175,112 reads, 31,668,459 reads pseudoaligned [quant] estimated average fragment length: 190.787 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,136 rounds 52401 SRR22215328.ke.tsv 34699 SRR22215328.se.tsv 87100 total ==> SRR22215328.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1828.21 985 16.3471 Potri.005G024800.1.v4.1 1035 845.213 249 8.9385 Potri.004G059700.1.v4.1 961 771.22 149 5.86192 Potri.007G009000.2.v4.1 1416 1226.21 0 0 Potri.003G141000.2.v4.1 2943 2753.21 236.167 2.60262 Potri.016G087400.1.v4.1 270 84.2236 1263 454.99 Potri.015G069301.1.v4.1 564 374.239 0 0 Potri.010G195200.1.v4.1 1773 1583.21 54 1.03487 Potri.012G127500.1.v4.1 977 787.213 4537 174.867 ==> SRR22215328.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 2887 Potri.001G233950.v4.1 14 Potri.001G122700.v4.1 590 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 197 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 4 SRR22215328 completed mapping pipeline successfully