Starting /dee2/code/volunteer_pipeline.sh SRR26075317 current disk space = 3053472215040 free memory = 1466614700 SRR26075317 SRAfilesize 2a9f365e47e7bad20458e6ae6e6b0238 SRR26075317.sra SRR26075317.sra file validated SRR26075317 is paired end SRR26075317 is conventional basespace SRR26075317 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075317_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.62275 37.0 37.0 37.0 37.0 37.0 2 36.5525 37.0 37.0 37.0 37.0 37.0 3 36.5955 37.0 37.0 37.0 37.0 37.0 4 36.6955 37.0 37.0 37.0 37.0 37.0 5 36.763 37.0 37.0 37.0 37.0 37.0 6 36.6845 37.0 37.0 37.0 37.0 37.0 7 36.6915 37.0 37.0 37.0 37.0 37.0 8 36.606 37.0 37.0 37.0 37.0 37.0 9 36.6235 37.0 37.0 37.0 37.0 37.0 10-14 36.663349999999994 37.0 37.0 37.0 37.0 37.0 15-19 36.6093 37.0 37.0 37.0 37.0 37.0 20-24 36.5998 37.0 37.0 37.0 37.0 37.0 25-29 36.4643 37.0 37.0 37.0 37.0 37.0 30-34 36.444599999999994 37.0 37.0 37.0 37.0 37.0 35-39 36.4116 37.0 37.0 37.0 37.0 37.0 40-44 36.36559999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.323800000000006 37.0 37.0 37.0 37.0 37.0 50-54 36.2156 37.0 37.0 37.0 37.0 37.0 55-59 36.1546 37.0 37.0 37.0 37.0 37.0 60-64 36.093500000000006 37.0 37.0 37.0 37.0 37.0 65-69 36.0737 37.0 37.0 37.0 37.0 37.0 70-74 36.0635 37.0 37.0 37.0 37.0 37.0 75-79 36.085300000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.9971 37.0 37.0 37.0 37.0 37.0 85-89 35.9289 37.0 37.0 37.0 37.0 37.0 90-94 35.8888 37.0 37.0 37.0 37.0 37.0 95-99 35.9674 37.0 37.0 37.0 37.0 37.0 100-104 35.856700000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.80460000000001 37.0 37.0 37.0 37.0 37.0 110-114 35.705499999999994 37.0 37.0 37.0 37.0 37.0 115-119 35.512899999999995 37.0 37.0 37.0 37.0 37.0 120-124 35.6511 37.0 37.0 37.0 37.0 37.0 125-129 35.423 37.0 37.0 37.0 37.0 37.0 130-134 35.300700000000006 37.0 37.0 37.0 37.0 37.0 135-139 35.1634 37.0 37.0 37.0 29.8 37.0 140-144 35.0021 37.0 37.0 37.0 27.4 37.0 145-149 34.9009 37.0 37.0 37.0 25.0 37.0 150-151 34.665 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 0.0 20 2.0 21 1.0 22 5.0 23 2.0 24 4.0 25 6.0 26 11.0 27 10.0 28 29.0 29 20.0 30 37.0 31 38.0 32 57.0 33 117.0 34 148.0 35 434.0 36 2855.0 37 222.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.90342757067801 12.859644733550162 8.206154615961973 41.030773079809855 2 18.5 14.499999999999998 35.775 31.225 3 17.4 17.775 30.099999999999998 34.725 4 22.900000000000002 22.400000000000002 24.0 30.7 5 25.674999999999997 29.099999999999998 23.75 21.475 6 22.175 33.15 22.275 22.400000000000002 7 16.775000000000002 28.15 38.824999999999996 16.25 8 19.575 24.325 31.2 24.9 9 17.075000000000003 24.6 34.725 23.599999999999998 10-14 20.626031301565078 28.8064403220161 27.826391319565978 22.741137056852843 15-19 19.245 27.72 28.439999999999998 24.595 20-24 20.419999999999998 28.050000000000004 27.694999999999997 23.835 25-29 20.04 26.77 28.325 24.865000000000002 30-34 19.564999999999998 28.16 27.43 24.845 35-39 21.05 27.575 26.985 24.39 40-44 19.755 28.235 27.71 24.3 45-49 20.645 27.025 27.955000000000002 24.375 50-54 21.044999999999998 27.52 27.77 23.665 55-59 20.49 28.110000000000003 27.965 23.435 60-64 20.325 28.115000000000002 27.439999999999998 24.12 65-69 21.05 27.229999999999997 27.845 23.875 70-74 21.48 27.6 27.465 23.455000000000002 75-79 19.830000000000002 28.544999999999998 27.215 24.41 80-84 20.695 28.345 26.58 24.38 85-89 21.72 27.165 27.065 24.05 90-94 21.834999999999997 27.555000000000003 27.075 23.535 95-99 20.9 27.105 27.565 24.43 100-104 21.485000000000003 27.935 27.355 23.225 105-109 21.43 28.165000000000003 26.479999999999997 23.925 110-114 20.919999999999998 27.47 26.775 24.834999999999997 115-119 21.27 27.49 26.525 24.715 120-124 21.545 28.255000000000003 25.895000000000003 24.305 125-129 22.05 28.12 26.235000000000003 23.595 130-134 21.65 27.800000000000004 26.119999999999997 24.43 135-139 21.25 27.725 26.895000000000003 24.13 140-144 22.645 27.400000000000002 25.85 24.104999999999997 145-149 22.545 27.495000000000005 25.775 24.185000000000002 150-151 23.075000000000003 27.6125 25.4875 23.825 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 1.5 19 2.0 20 1.0 21 1.5 22 1.0 23 1.5 24 5.0 25 3.5 26 0.5 27 2.0 28 2.5 29 4.5 30 10.5 31 15.5 32 20.5 33 40.5 34 51.0 35 47.5 36 80.5 37 96.5 38 120.0 39 162.0 40 168.5 41 188.5 42 221.0 43 243.0 44 255.5 45 260.5 46 269.0 47 273.0 48 240.5 49 209.5 50 176.0 51 149.0 52 150.5 53 116.0 54 77.0 55 71.0 56 55.0 57 35.5 58 37.5 59 34.5 60 19.0 61 10.0 62 9.0 63 11.0 64 9.0 65 5.0 66 4.0 67 8.5 68 7.5 69 2.5 70 1.5 71 2.0 72 2.0 73 0.0 74 1.0 75 2.5 76 1.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.35 #Duplication Level Percentage of deduplicated Percentage of total 1 63.214581607290796 38.15 2 21.333885666942834 25.75 3 8.699254349627175 15.75 4 3.6868268434134217 8.9 5 1.3256006628003314 4.0 6 0.7870753935376968 2.85 7 0.4971002485501243 2.1 8 0.12427506213753108 0.6 9 0.2071251035625518 1.125 >10 0.12427506213753108 0.775 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCAGTACTAAGTCCAAATGATGTTGCGGCAGCAATTGGAAACAAAGGAT 11 0.27499999999999997 No Hit CATCCAACTTGTATTTCTTCATAAGTTCATGCATCTTCTCTATCTCCAGA 10 0.25 No Hit CCCCGGTCAAGATAGCAGCCTGGACTGCAGCACCATATGCAACAGCCTCA 10 0.25 No Hit GCCGCAAAGTCTGGCAACAGTTGTGCTAAAAACCTCTTTCGGTTCTGAAT 9 0.22499999999999998 No Hit CTGGTACTTCCTGATTTCTCTCAGCGCCACTGTTCCTGGCCTGAAACGGT 9 0.22499999999999998 No Hit TAATTTTTCACGGGAATAATTACAAAATTCAAAAGTATTCTTAGCTCTTA 9 0.22499999999999998 No Hit GTCTCCTTCGCTGCCGAACGAGGATTGAGCTGCCCCAGCCACTAGCTTGG 9 0.22499999999999998 No Hit GTTTGCTGCCATTTCGCAGAAAGAGCAATGGAAGTTGTTGAAACATCAGT 9 0.22499999999999998 No Hit CGGGAAACCTCAACTTTACAGTCTATAAATAAATGCATACAAAATAATAA 8 0.2 No Hit GTGCAACTGCGTTAGAACTTCAACCTCTCTCAAGAACTCCTGTTTTTTGT 8 0.2 No Hit CTGAGATTTTCCAAGGTCTTTCCACCACACCAGTGGCAGCACCATCTGAG 8 0.2 No Hit GTCCACATTTGAGGAAGGAGTTTGAAGGAAGAAAGGGTGTCTTGAAAGAC 7 0.17500000000000002 No Hit GGGCGATTTGTATCTTTGTGACCGCAGCAAAATGATGAATTATCACCACA 7 0.17500000000000002 No Hit CTGCATTCAAAGTCTCTGTGGAACTAAAATTCTTCAAGCAGCTTGTTATG 7 0.17500000000000002 No Hit AAACATTGTCACGACCTACATGACTATGCTATTCCCATGTCCAAATCCTA 7 0.17500000000000002 No Hit CAACGTCCTTGAGGGCGCAGATCTGCTCCTCTCCCATGGAGGACATCACA 7 0.17500000000000002 No Hit GGTCTGTCTGTTTATGCCGCGTGATTGACCATACAAACAAATATACACTA 7 0.17500000000000002 No Hit ATAGGGGACGAACCCGGGGCTGCGCGTGACGAACCACTGAGTGGCCGTTA 7 0.17500000000000002 No Hit GTTGGTACAGATGGAGAGGGATGGAGGGTGGCTGAGCGGGTAAGGTTCTT 7 0.17500000000000002 No Hit GGGAGCGAAAGGGCCAACGAGGAGGGAGATGGAGACCCAGAAGAGGAGGG 7 0.17500000000000002 No Hit CTTTGGTCGGCAGGTTTTCCTAGGCATACCTTGATTTTACCCCCAACAAT 7 0.17500000000000002 No Hit CAGGAACGTTGAGAATCCTCCATGATGGTTTCTCCTCAGCAGGATCTAAC 7 0.17500000000000002 No Hit CCTACACGAAGAGCAAACTGATCCACCCATCTTGGTGGAGCTCACATAGA 7 0.17500000000000002 No Hit GCCATCACCCCTGGATTTAGTCTTCACAGGAATAGGATTTTGCTGGTCGG 6 0.15 No Hit CCTCCCATGTCCGGATCCGTACCCCGTCTCAGTCCTCTCTTCATGTTCGC 6 0.15 No Hit CCAAGAGTCGGACCTAGCACCTTGCACCGGCAAGGGTTGCTGGTTGTGCC 6 0.15 No Hit GTCCACGCCAAGCTGACCATACTTGTTCCAACCCCAGGAAAATACTTGGC 6 0.15 No Hit GATTCTACAAACATACAATGTTTCAATCTGGAGTGAAGAAAGAATTTGTA 6 0.15 No Hit GCAGCAACATTTCCATGTACATCAGTGGTCACTAGTTCAGTTTTTCCATC 6 0.15 No Hit AGGGATTGAGAAATCAAGGGCAATGCAGCAGGGTCATCGTAATGGACGGT 6 0.15 No Hit TTCCACCTCCTTGGATAAATCTTCCAGCTTCTCTACCACCCCCATTAGTG 6 0.15 No Hit ATTATCAAGAAACCAGACAGGACCAACAGCGTTAACATGCGGCGGCTGCT 6 0.15 No Hit CACATGTTGTTCAGCTTTAAGAACTTCGTTTTCCACATCTGCCTCATGGT 6 0.15 No Hit ACTGCTTCCTCCGACTCCGTCTCTCCACCCTATTAGGTTGTTCATGACTC 6 0.15 No Hit CCTGCCACCAATTTCCTCTTGAAGCACTGCCTGTGTCATCTTCAGGTCCA 6 0.15 No Hit GTTAGAGGGCCTTCAGGGTTGTAACCATACTCAGACTCTGCTTCTTCAAG 6 0.15 No Hit GTACCAGCATATGGAAAAGTATCCAAGCTTATGTCCATTAAAGAATATGC 6 0.15 No Hit CCCAATTTCTCACTCTTTTCACTACTGCAAGAACAACTCTTCTCACTACT 6 0.15 No Hit CAGCCAGGCGAATCAAGGGAAGCCTGCAGTTGGAAGTCTTGCAGACAATA 6 0.15 No Hit CCCAGTTGCTACTTGCCCACCAGCCATTTTTCTTGATAAGTTGCTCCAAA 6 0.15 No Hit CTCCGAGCCATCCACAGAGCAACCAAGCACATCGTGGCTAGCCAAGAGCC 6 0.15 No Hit GTACACAGACAAAAGATAAGGTTGCACAATCCCACCTAAGAATCCAAGGT 6 0.15 No Hit CAAGATTACACTCGCGCACCAAAACGGATAATAATTTCCAAGATTACACT 5 0.125 No Hit ATTCAGTGTTCTCTTCAGTTTTGCTCATTTTGATTCTCTGTGATTTTGGT 5 0.125 No Hit CAGCAGTACAAGTACACACTACCATTAGCTCTCTCCCCCTCTTATATAAT 5 0.125 No Hit CCGATCACAAAGTTTTCGAACACGTCTGAGGGTGTAATGGTTAGCGTTTC 5 0.125 No Hit CCTCGAGGCTAGCTGGAAAAATACATTTGGCCCATTGGCACCCAGTTGTA 5 0.125 No Hit GACGCTTCTTAGAGACACCAACAGTCTTTCCACGACGGCCAGTAGTCTTT 5 0.125 No Hit ATCATCTATAAGCACTTCCCTCTTCTTAATTCTATCAAAGTTGTCACTAT 5 0.125 No Hit CCCCTCTCCTTCCATTGACGTAAGCATGCATCAATCTGGCCTTGGGTCCT 5 0.125 No Hit GGAGCTGCAATGTTGAGATCAATATTAATGCTTGAAGAACCAGAAGTTGC 5 0.125 No Hit CATTCATTGGGGTGTTGATGAACCAAGGAGCGACGGAATTAACCCTAATA 5 0.125 No Hit GCATGAGCATGAAATGAGGGATTTATTGTACAGTAAAGAGTGTGGTTGAA 5 0.125 No Hit ACCAAAAACATGCTTTGCTAGCTGAATTATTTGGGTACCAACTCCACCAG 5 0.125 No Hit GTCATGGAAATCTCCGGCATACCGATAAACATCATCAAAGCCAAGGAGTA 5 0.125 No Hit CATGAATTGCACAAAGCCATGTCCCTGGTTCCTGTCAACAGCTTGGACCC 5 0.125 No Hit CTCGGAGCTCGTTGTTGTGATCTACCACATTAAGAGCACTAGTTGAGTTG 5 0.125 No Hit ATTGTATCCATACCATATGGGGCAATGCCTGCTCAGTAAAATCTCAATAG 5 0.125 No Hit GGCTCGTCTGCTGGAGGCTGAAAGTTCTCAGGTAAAGGTTGCCCACATTC 5 0.125 No Hit GCCGTTATAACGTTGCTGTTCGCCCAGTGGGCTCCGCGCGTGAGCACTTC 5 0.125 No Hit CCTCAGAGAGCTCCATTTCACTAGCAGAAAGACACCCGGCGAAGACCCGG 5 0.125 No Hit CAGTAGGATAGGCTTTAAATAAAGGTAGGAACCGCTTCTGGCAGTACGCA 5 0.125 No Hit GTCTTCCTTCACAATTTCTCAAGTGTAGAACAACATCACTAGTGTTCTCA 5 0.125 No Hit CAGCAAGATCCACAGTGACCTTGATTCTTAACGGGACTGACTATGCCTTC 5 0.125 No Hit ATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTAGA 5 0.125 No Hit GTCCACTTGTCTTGTTGCCAATATTCCACACTCTTGTCAAAATCAACTCG 5 0.125 No Hit ATGGGAAGCCAGAAAACTTTCTTGGGTGCTTCATTTGGAGAGAACATGTT 5 0.125 No Hit CTCTCCTGAAATACTTCTGGCTCTAGCTATCCCTCCCATTTCCATGTCCT 5 0.125 No Hit GTTGCGAGAGGATACGGTGACTTGGAAGTTGGAAGTGATGAAGTTTGGAT 5 0.125 No Hit GCCACATTTACTGAGTGACTCCCGGTCTTAACATATATAATAAGGCTATT 5 0.125 No Hit TATTTTGTTTCCTGGGCCTATCCTCTGTTCACTGTCAATCCAGTATGAAG 5 0.125 No Hit GGTGGACCTCTTTTGAAAAGATGATCTTCTAAGCTTCTGTTGGGCATATA 5 0.125 No Hit GCAAGAAGTGAATATCTATTGTTGGGTTGGCAGAAGGGATGGGAGGAACG 5 0.125 No Hit GTCAGGTTTGAATCGTTCTGTCTTCATTATCTTATCTAATTGCTCATCTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.175 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.2125 0.0 0.0 0.0 0.0 78-79 0.2625 0.0 0.0 0.0 0.0 80-81 0.3375 0.0 0.0 0.0 0.0 82-83 0.6 0.0 0.0 0.0 0.0 84-85 0.7375 0.0 0.0 0.0 0.0 86-87 0.9624999999999999 0.0 0.0 0.0 0.0 88-89 1.0625 0.0 0.0 0.0 0.0 90-91 1.1375 0.0 0.0 0.0 0.0 92-93 1.25 0.0 0.0 0.0 0.0 94-95 1.425 0.0 0.0 0.0 0.0 96-97 1.7 0.0 0.0 0.0 0.0 98-99 2.0 0.0 0.0 0.0 0.0 100-101 2.4375 0.0 0.0 0.0 0.0 102-103 2.7375 0.0 0.0 0.0 0.0 104-105 3.1 0.0 0.0 0.0 0.0 106-107 3.4875 0.0 0.0 0.0 0.0 108-109 3.9000000000000004 0.0 0.0 0.0 0.0 110-111 4.1125 0.0 0.0 0.0 0.0 112-113 4.550000000000001 0.0 0.0 0.0 0.0 114-115 4.9625 0.0 0.0 0.0 0.0 116-117 5.65 0.0 0.0 0.0 0.0 118-119 6.15 0.0 0.0 0.0 0.0 120-121 6.65 0.0 0.0 0.0 0.0 122-123 7.15 0.0 0.0 0.0 0.0 124-125 7.9750000000000005 0.0 0.0 0.0 0.0 126-127 8.425 0.0 0.0 0.0 0.0 128-129 9.025 0.0 0.0 0.0 0.0 130-131 10.175 0.0 0.0 0.0 0.0 132-133 11.1125 0.0 0.0 0.0 0.0 134-135 11.975000000000001 0.0 0.0 0.0 0.0 136-137 13.149999999999999 0.0 0.0 0.0 0.0 138-139 13.8875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTGAGG 10 0.006830828 145.0 8 CACATTT 10 0.006830828 145.0 4 CAATACG 10 0.006830828 145.0 2 ATACGAT 10 0.006830828 145.0 4 GTCCACA 10 0.006830828 145.0 1 ATTTGAG 10 0.006830828 145.0 7 AATACGA 10 0.006830828 145.0 3 >>END_MODULE SRR26075317 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075317_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1285 37.0 37.0 37.0 37.0 37.0 2 36.245 37.0 37.0 37.0 37.0 37.0 3 36.2395 37.0 37.0 37.0 37.0 37.0 4 36.258 37.0 37.0 37.0 37.0 37.0 5 36.3395 37.0 37.0 37.0 37.0 37.0 6 36.246 37.0 37.0 37.0 37.0 37.0 7 36.194 37.0 37.0 37.0 37.0 37.0 8 36.22 37.0 37.0 37.0 37.0 37.0 9 36.2325 37.0 37.0 37.0 37.0 37.0 10-14 36.2669 37.0 37.0 37.0 37.0 37.0 15-19 36.2149 37.0 37.0 37.0 37.0 37.0 20-24 36.135200000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.0831 37.0 37.0 37.0 37.0 37.0 30-34 35.96509999999999 37.0 37.0 37.0 37.0 37.0 35-39 35.9348 37.0 37.0 37.0 37.0 37.0 40-44 35.8185 37.0 37.0 37.0 37.0 37.0 45-49 35.8579 37.0 37.0 37.0 37.0 37.0 50-54 35.711800000000004 37.0 37.0 37.0 37.0 37.0 55-59 35.7658 37.0 37.0 37.0 37.0 37.0 60-64 35.756499999999996 37.0 37.0 37.0 37.0 37.0 65-69 35.6568 37.0 37.0 37.0 37.0 37.0 70-74 35.7005 37.0 37.0 37.0 37.0 37.0 75-79 35.486200000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.6195 37.0 37.0 37.0 37.0 37.0 85-89 35.541 37.0 37.0 37.0 37.0 37.0 90-94 35.46665 37.0 37.0 37.0 37.0 37.0 95-99 35.5681 37.0 37.0 37.0 37.0 37.0 100-104 35.4021 37.0 37.0 37.0 37.0 37.0 105-109 35.420899999999996 37.0 37.0 37.0 34.6 37.0 110-114 35.3293 37.0 37.0 37.0 34.6 37.0 115-119 35.3654 37.0 37.0 37.0 37.0 37.0 120-124 35.1485 37.0 37.0 37.0 29.8 37.0 125-129 35.24765000000001 37.0 37.0 37.0 32.2 37.0 130-134 35.1078 37.0 37.0 37.0 27.4 37.0 135-139 34.93665 37.0 37.0 37.0 25.0 37.0 140-144 34.93560000000001 37.0 37.0 37.0 25.0 37.0 145-149 34.91005 37.0 37.0 37.0 25.0 37.0 150-151 34.536625 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 3.0 14 6.0 15 3.0 16 6.0 17 2.0 18 3.0 19 2.0 20 7.0 21 4.0 22 6.0 23 6.0 24 13.0 25 11.0 26 16.0 27 13.0 28 16.0 29 27.0 30 25.0 31 42.0 32 60.0 33 94.0 34 238.0 35 727.0 36 2486.0 37 183.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.24412206103052 22.0360180090045 12.481240620310155 27.238619309654826 2 25.874999999999996 28.525 28.425 17.175 3 19.2 29.849999999999998 31.900000000000002 19.05 4 25.174999999999997 31.075000000000003 22.75 21.0 5 25.45 36.7 21.425 16.425 6 20.7 39.475 21.7 18.125 7 21.975 22.3 34.575 21.15 8 24.175 27.625 26.375 21.825 9 24.45 23.75 28.7 23.1 10-14 23.59 28.749999999999996 26.515 21.145 15-19 24.335 28.305000000000003 27.01 20.349999999999998 20-24 24.38 28.555000000000003 26.590000000000003 20.474999999999998 25-29 24.36 27.305 27.384999999999998 20.95 30-34 24.315 28.365000000000002 26.26 21.060000000000002 35-39 24.04 28.395 26.07 21.495 40-44 24.93 28.435 25.655 20.979999999999997 45-49 24.5 27.705000000000002 27.400000000000002 20.395 50-54 24.64 27.779999999999998 27.474999999999998 20.105 55-59 24.09 27.46 28.12 20.330000000000002 60-64 25.195 27.54 27.139999999999997 20.125 65-69 24.195 28.449999999999996 26.119999999999997 21.235 70-74 24.23 27.77 26.810000000000002 21.19 75-79 24.01 28.095 27.325 20.57 80-84 24.205 28.09 26.88 20.825 85-89 23.810000000000002 28.225 27.175 20.79 90-94 24.576228811440572 27.496374818740936 26.64633231661583 21.281064053202662 95-99 24.335 27.935 27.365000000000002 20.365 100-104 25.31 27.68 26.61 20.4 105-109 25.485000000000003 27.68 26.555 20.28 110-114 25.355 29.335 25.515 19.794999999999998 115-119 25.39 28.265 26.41 19.935 120-124 25.047504750475046 27.81278127812781 26.512651265126514 20.627062706270628 125-129 25.891294564728234 27.76138806940347 26.73133656682834 19.615980799039953 130-134 25.525 29.080000000000002 25.495 19.900000000000002 135-139 27.209523333166608 28.750062521882658 25.113789826439252 18.92662431851148 140-144 27.823911955977987 28.52926463231616 24.87743871935968 18.769384692346172 145-149 27.21769149947466 28.023215089808375 25.341471956771898 19.417621453945063 150-151 27.67979987492183 26.79174484052533 27.31707317073171 18.211382113821138 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.5 6 1.0 7 1.0 8 1.0 9 1.0 10 1.0 11 0.5 12 0.0 13 0.0 14 0.0 15 1.0 16 1.5 17 0.5 18 0.0 19 0.0 20 0.0 21 1.5 22 2.5 23 2.0 24 1.0 25 0.5 26 1.5 27 1.5 28 3.0 29 7.0 30 9.0 31 12.0 32 17.5 33 25.5 34 34.5 35 48.5 36 70.0 37 88.5 38 104.0 39 146.5 40 194.5 41 229.0 42 277.0 43 272.5 44 251.0 45 268.5 46 252.5 47 240.0 48 263.5 49 238.5 50 173.5 51 134.0 52 120.5 53 98.0 54 71.0 55 69.0 56 52.0 57 38.0 58 40.5 59 27.0 60 19.5 61 17.5 62 10.5 63 7.0 64 4.5 65 2.5 66 3.5 67 3.0 68 1.5 69 1.0 70 5.5 71 6.5 72 1.5 73 0.5 74 0.5 75 0.0 76 0.5 77 0.5 78 0.5 79 1.0 80 0.5 81 0.5 82 1.0 83 1.0 84 1.0 85 0.5 86 0.5 87 0.5 88 0.0 89 0.0 90 1.0 91 1.5 92 0.5 93 1.5 94 1.5 95 0.5 96 0.5 97 0.5 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.005 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.01 125-129 0.005 130-134 0.0 135-139 0.034999999999999996 140-144 0.05 145-149 0.065 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.3 #Duplication Level Percentage of deduplicated Percentage of total 1 64.84502446982056 39.75 2 20.187601957585642 24.75 3 8.523654159869494 15.675 4 3.507340946166395 8.6 5 1.3050570962479608 4.0 6 0.6525285481239804 2.4 7 0.40783034257748774 1.7500000000000002 8 0.28548123980424145 1.4000000000000001 9 0.1631321370309951 0.8999999999999999 >10 0.12234910277324632 0.775 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCAATTCTGGCACAGAAGATGTGTTTTCCTTCCTCAATTCACTCATAAAT 11 0.27499999999999997 No Hit GAACATGGATCTCTTCAGGAAGTGTATGGAGCCTGTAGAGAAGTGTTTGA 10 0.25 No Hit TTTTTTCCATGGCAAGGCTGGACCGGGTCAAGAACATATCAGGATTGGTA 10 0.25 No Hit ATCCACAATAGGGTAGCACAGAAATTGTCGTCCACGATGGTGGATCATAC 9 0.22499999999999998 No Hit AAACAAGAAAATTATCTGCAAGCCTCTCTTGATTCTCGCTGCTGTTTCCT 9 0.22499999999999998 No Hit TGTGAAAACCTCTCCTTATCAGGCACTCACCAAACTTTTCTTCCTAACCA 9 0.22499999999999998 No Hit ATTTTCCCAACTTACCTATTTCAATGGCTCGTACAAAGCAAACAGCAAGA 9 0.22499999999999998 No Hit GAAGCAAAAGATTTGCTTGCTAAAGAAGCTAATGGAAGACAGATAGCAGA 8 0.2 No Hit CTCAAACAGTAACATTAATGAGAAACAAAATGAAGGATTGTCCAGTAGCT 8 0.2 No Hit CACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGTGG 8 0.2 No Hit CAACAACACAAAACTTCTTAAAACCAAAACCAAAGGTGAAATCTTTGAGG 8 0.2 No Hit ATTTGTGAGCTTGCTGACTGAGAATGGCAATACCAAGGATGACCTGAGGC 8 0.2 No Hit CAACGGTGCAATCTCAGCTAAACCGACGCAGTTTTGCTCCTTGGATTCTG 8 0.2 No Hit GCTGATGGCTTAGTTATATTTTCTGGCCATCCAGGTCAGCAAAGAGTTAA 8 0.2 No Hit AAGCGTGTTGGACCCGAAAATACTATATCTTGAAATAATGATTTTACTTT 7 0.17500000000000002 No Hit ACTTGATCATGTTGCTGTTAGCATGCCCTGTGGAAGGAATATAATCTTTG 7 0.17500000000000002 No Hit CTTCGTTAATTTTCCCTTTTGCAATCCATTGCTAGCCTCACAATTCTCGA 7 0.17500000000000002 No Hit AATTAACCAAATTAATTTGTTTTCTCTTCTCTCACTGCCAAAAACAAAAA 7 0.17500000000000002 No Hit GGACGCCAGACCTCGATTCCTCTTTCAATCTCGCCCGATCCCATCTTCAC 7 0.17500000000000002 No Hit GACCAAAGCATGCTCAGGCAAATGGTGTTTCAAAGGAGCCTTTGGTTACC 7 0.17500000000000002 No Hit AATAGTTCCGTGCCTTGTGATGCAATAACATGTGTTCCTGAGATCTTGCC 7 0.17500000000000002 No Hit ATTCACACGGATTAGATTCCCATCTTTGGCTAACGTGGTCCAAGTCTCAG 7 0.17500000000000002 No Hit TAACTATAGGAGTTTAGAACGACAACCAATTTGCAATGCACAAATATATG 7 0.17500000000000002 No Hit GGCTGAAGGGCCCAAGGAAGAAGAGGAATCCGAGAAGTCAGTGTCGGAAG 7 0.17500000000000002 No Hit CAGGAAATCACGCGTAGCTCCTGAAGGTTGCTTCTCAGTCTACGTTGGAC 6 0.15 No Hit AGAAATCGGTCACTTACTAGAGGAGAGATCGATGCTTACTGGAGATTAAA 6 0.15 No Hit TATATATACTCTTCGCCTTCGGTGGTTGATTTGGATGGTGATGGGAACTT 6 0.15 No Hit GAGAATAAGAGTGGTGATGGTAGTGAGAAAAAGCCTATTGCTTTTGAAGA 6 0.15 No Hit GTGTCCTTTCAAATTAGGGAAACTTGATAATGCTGAGGGATTTGCAGTAG 6 0.15 No Hit TGTATATGTATTTGGTGGGAATCAGTTTGGACAATTGGGTACTGGAGCTA 6 0.15 No Hit AAGCCGAGCAAGCATGGGTCTGGTTATGGACGTAAACAAGAATCGGATTA 6 0.15 No Hit CAAGTCGACCAGTTTCTCCTTGAGGCCTTGCAGAACACTCGCGAACGCCT 6 0.15 No Hit TAGGATACTCTGTGCAGTTCCGAACTCCCGCCTTGTGGTGAAATGCAAGC 6 0.15 No Hit GAAATACACCAAAGAGTGTGCAGATCAAGTTATTCAATCTATTGGTGTTG 6 0.15 No Hit AGAACCTTGTGATACCACTTCACAACCCCTTCACAAGAAAGAAGCTGATA 6 0.15 No Hit ATCAACCACCACAACCGAACTTCTCTCGTCCTTATCTCCATTCTTCCATA 6 0.15 No Hit GAAAAAAAGGAGTTTCAGAAGGTTTATTTGCTTCAGGCTTTGCAGGCAAT 6 0.15 No Hit CTTAGTTCATCAATCTATTATGGGGGTCAAGATATCTATCATCATCCTCA 6 0.15 No Hit TGAAAATGCTTGCTTTCTTGCAACTTTTAGAATACCTATACGTCTTGGAG 6 0.15 No Hit TGTAGCTTGTAAAGAACTAAGCTTGATTCTACGAGAAGCTTATAGCAAAC 6 0.15 No Hit AGGAAAAGTTGGATCTTATGTTAGTTGTTGGCGGGTGGAACTCAAGTAAC 5 0.125 No Hit TCAAGCTGAGCCTGCTGCTCCTGTTATTGCTACATCAACTACTACAGGGT 5 0.125 No Hit GTTGCTCTTGGTATGGCCTCTACTGGAGCTGGAAAAGGGGGAGAAGTTGC 5 0.125 No Hit GCTGCCCAGTTGCCCACAAAGAACCCAGATGCACCCATGATGCTGGACCG 5 0.125 No Hit CATAGATTTGTGTTGATATAGAAAACAATGGCACTACATGGAAAGATTGA 5 0.125 No Hit GTATCGTGGTGCTAAGGCGAAGACCAACTTTCCAATAGCGGAGAAGGTGG 5 0.125 No Hit CATCTCTCCAACATAACAAAAACTCTCTCCGCCTCACGACACAAAGACCT 5 0.125 No Hit TATGAGAAAAGCTTTCGACAGAAGATCAGACCAACATGTCTTGTAGAACT 5 0.125 No Hit GTTTCGATGAATCCAATCCAATCAAACTCGTATCTGACCGTCTCCATGAC 5 0.125 No Hit ATCATCTTTCGCAACCAGCTGTTGAATGTGTATGCCCCAAAGTATGAAAC 5 0.125 No Hit GGAGTTTGGGAATGGAAGAAATTCCGATCGGAGGAAGGTAAACTGGTATT 5 0.125 No Hit GCAGAAGGAAACGTTTCAAGATATGTGAAGTTAACAAAAGAACAAACTGG 5 0.125 No Hit GTTAAAGGGGCATGACAAACAGTTCATCGTCATCAAAAAAGAACGGTCAA 5 0.125 No Hit TGAAGACAAGACTGAATGGGGCAAAGAGATTGGATGGATCTATGGTTCTG 5 0.125 No Hit GCAGTAAGGATTGAAGGGTTTGATTGTCTCCGCAGAGATGGGCGAAACTG 5 0.125 No Hit GCAAACACAACTCACCTCTACTTCTCTTTCTATCTACTCCCTTCTCCAGT 5 0.125 No Hit AACTGGGTTCTCTGCCGGCAAATCTATCCTTGTTTTAGGAGGTGCTGGTG 5 0.125 No Hit CACTGCTCTTGTCACTGGTGGAACCAAAGGGATCGGATATGCTGTTGTGG 5 0.125 No Hit TGATGATGAAAAAACTGACTCCAATTTGTCGAAACCAGAAAGCAGAACAG 5 0.125 No Hit CGCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGG 5 0.125 No Hit GAACAATATAACAAAGCAGATTTCCCAGTGGAATATGTTGATGCTAAATT 5 0.125 No Hit GCGCATTTTTCTACACGACCAAGTGTTGGCACAATTTCGGCCTCCCTCAG 5 0.125 No Hit CAGCTGAGATTGAGACAAGCAATTGCAAAATTGCATTCCTTCGCTTAGGC 5 0.125 No Hit GGTCCTTGAACAATATCAAGTATATGGGGATGCTGTTCTGGGAACGTATG 5 0.125 No Hit CCTAACCTTCCTCCTCATTGCCCTCATCATAATCCTCATAGTTTGGGCCA 5 0.125 No Hit GCAATGTACTCAAATCTAGCAAAGGGCCAATTATTTCCCTTGGCTACCAG 5 0.125 No Hit AAAGCGCCCAGGGTTGCTCTTCCTCAAATTCATGCCTTTATGGAGTCAAA 5 0.125 No Hit CGAGGGAGGAGTTTATTCGGGTGCTTTGTTCTACTCCTTGGCTTTGATGA 5 0.125 No Hit GTAGTGTTAGTGGAGGAAATGAAGCTTGCTTTGTCAATGAACGAGTCTGA 5 0.125 No Hit GAACAATACCTCAAAAAGTACCATCCGCAAGGTTTCAAGGCAAGTTGCAT 5 0.125 No Hit AATGCATTGGACATGAAGTTGAGAGATGATTTGGAGCGTTTGAAGAAGAT 5 0.125 No Hit GAGCGAGAGTGACTCCTTATGTTGACGATTCCCTTGTCATTGATGCTAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.175 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.2125 0.0 0.0 0.0 0.0 78-79 0.2625 0.0 0.0 0.0 0.0 80-81 0.3375 0.0 0.0 0.0 0.0 82-83 0.575 0.0 0.0 0.0 0.0 84-85 0.7125 0.0 0.0 0.0 0.0 86-87 0.9375 0.0 0.0 0.0 0.0 88-89 1.0499999999999998 0.0 0.0 0.0 0.0 90-91 1.15 0.0 0.0 0.0 0.0 92-93 1.275 0.0 0.0 0.0 0.0 94-95 1.45 0.0 0.0 0.0 0.0 96-97 1.725 0.0 0.0 0.0 0.0 98-99 2.0250000000000004 0.0 0.0 0.0 0.0 100-101 2.45 0.0 0.0 0.0 0.0 102-103 2.7249999999999996 0.0 0.0 0.0 0.0 104-105 3.075 0.0 0.0 0.0 0.0 106-107 3.475 0.0 0.0 0.0 0.0 108-109 3.9000000000000004 0.0 0.0 0.0 0.0 110-111 4.1125 0.0 0.0 0.0 0.0 112-113 4.6375 0.0 0.0 0.0 0.0 114-115 5.15 0.0 0.0 0.0 0.0 116-117 5.85 0.0 0.0 0.0 0.0 118-119 6.3375 0.0 0.0 0.0 0.0 120-121 6.824999999999999 0.0 0.0 0.0 0.0 122-123 7.3125 0.0 0.0 0.0 0.0 124-125 8.0875 0.0 0.0 0.0 0.0 126-127 8.525 0.0 0.0 0.0 0.0 128-129 9.15 0.0 0.0 0.0 0.0 130-131 10.225000000000001 0.0 0.0 0.0 0.0 132-133 11.175 0.0 0.0 0.0 0.0 134-135 12.0625 0.0 0.0 0.0 0.0 136-137 13.2375 0.0 0.0 0.0 0.0 138-139 13.95 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAGCAA 10 0.006830828 145.0 8 TAGCTTG 10 0.006830828 145.0 3 GCTTGTA 10 0.006830828 145.0 5 CTTGTAA 10 0.006830828 145.0 6 AGACAGC 10 0.006830828 145.0 6 GTAGCTT 10 0.006830828 145.0 2 TGTAAAG 10 0.006830828 145.0 8 GTAAAGA 10 0.006830828 145.0 9 >>END_MODULE Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836913 spots for SRR26075317.sra Written 836913 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra Read 836912 spots for SRR26075317.sra Written 836912 spots for SRR26075317.sra SRR ids: ['SRR26075317.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd__chkcnsr SRR26075317.sra spots: 16738241 blocks: [[1, 836912], [836913, 1673824], [1673825, 2510736], [2510737, 3347648], [3347649, 4184560], [4184561, 5021472], [5021473, 5858384], [5858385, 6695296], [6695297, 7532208], [7532209, 8369120], [8369121, 9206032], [9206033, 10042944], [10042945, 10879856], [10879857, 11716768], [11716769, 12553680], [12553681, 13390592], [13390593, 14227504], [14227505, 15064416], [15064417, 15901328], [15901329, 16738241]] SRR26075317 file size 6175528 SRR26075317 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075317 SRR26075317_1.fastq SRR26075317_2.fastq Input file: SRR26075317_1.fastq Paired file: SRR26075317_2.fastq trimmed: SRR26075317-trimmed-pair1.fastq, SRR26075317-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 18:33:59 2025 >> started Tue Feb 11 18:34:20 2025 >> done (21.727s) 16738241 read pairs processed; of these: 48 ( 0.00%) short read pairs filtered out after trimming by size control 72525 ( 0.43%) empty read pairs filtered out after trimming by size control 16665668 (99.57%) read pairs available; of these: 3036735 (18.22%) trimmed read pairs available after processing 13628933 (81.78%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 2 0.00% 20 5 0.00% 21 10 0.00% 22 9 0.00% 23 9 0.00% 24 16 0.00% 25 16 0.00% 26 9 0.00% 27 11 0.00% 28 19 0.00% 29 21 0.00% 30 16 0.00% 31 28 0.00% 32 23 0.00% 33 20 0.00% 34 29 0.00% 35 24 0.00% 36 27 0.00% 37 33 0.00% 38 46 0.00% 39 39 0.00% 40 52 0.00% 41 69 0.00% 42 48 0.00% 43 66 0.00% 44 46 0.00% 45 61 0.00% 46 75 0.00% 47 92 0.00% 48 96 0.00% 49 131 0.00% 50 107 0.00% 51 134 0.00% 52 160 0.00% 53 177 0.00% 54 172 0.00% 55 198 0.00% 56 297 0.00% 57 227 0.00% 58 296 0.00% 59 402 0.00% 60 440 0.00% 61 534 0.00% 62 562 0.00% 63 629 0.00% 64 786 0.00% 65 914 0.01% 66 965 0.01% 67 1109 0.01% 68 1160 0.01% 69 1462 0.01% 70 1542 0.01% 71 1932 0.01% 72 2061 0.01% 73 2466 0.01% 74 2777 0.02% 75 2988 0.02% 76 3555 0.02% 77 3873 0.02% 78 4061 0.02% 79 4701 0.03% 80 5346 0.03% 81 5954 0.04% 82 6776 0.04% 83 7540 0.05% 84 8495 0.05% 85 9472 0.06% 86 9939 0.06% 87 10877 0.07% 88 11167 0.07% 89 12171 0.07% 90 12650 0.08% 91 14283 0.09% 92 15454 0.09% 93 16675 0.10% 94 18422 0.11% 95 19024 0.11% 96 20674 0.12% 97 22045 0.13% 98 22328 0.13% 99 23278 0.14% 100 24206 0.15% 101 25665 0.15% 102 27008 0.16% 103 28031 0.17% 104 29880 0.18% 105 31488 0.19% 106 32627 0.20% 107 34124 0.20% 108 35310 0.21% 109 35668 0.21% 110 36506 0.22% 111 37447 0.22% 112 38519 0.23% 113 39262 0.24% 114 41257 0.25% 115 43765 0.26% 116 44405 0.27% 117 46728 0.28% 118 46715 0.28% 119 47859 0.29% 120 48352 0.29% 121 49778 0.30% 122 49459 0.30% 123 51510 0.31% 124 53215 0.32% 125 53776 0.32% 126 55736 0.33% 127 56983 0.34% 128 58256 0.35% 129 59670 0.36% 130 61134 0.37% 131 60945 0.37% 132 61616 0.37% 133 62059 0.37% 134 62936 0.38% 135 63865 0.38% 136 65501 0.39% 137 66480 0.40% 138 68496 0.41% 139 71143 0.43% 140 70681 0.42% 141 71799 0.43% 142 71745 0.43% 143 71442 0.43% 144 72543 0.44% 145 73340 0.44% 146 74178 0.45% 147 74857 0.45% 148 76739 0.46% 149 78199 0.47% 150 79425 0.48% 151 13628933 81.78% 16665668 reads passed initial QC criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=3.75 fanout-score-rank=19 prefix-density=0.34 prefix-fanout=3.2 sequence=AGGAAACCTCCT criterion=fanout-score sequence-density=0.02 sequence-density-rank=37 fanout-score=55.47 fanout-score-rank=1 prefix-density=0.15 prefix-fanout=8.2 sequence=TTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAATATGACAAGGAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATGGAGCTATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATTATTTTTAGAAATCCCATACTGCCACCGAATATTCAGTCCTTTAAGAAATCGAACAGCATACCCAACATAGTAAAAACCATCAATAATGCAAATACCGTTACCACAAGTGCAAATACTCCCATTCCTACCTCTCC criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=2.25 fanout-score-rank=32 prefix-density=0.36 prefix-fanout=2.2 sequence=TGGTTTTACTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=71.73 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=4.4 sequence=ACAAAGCAGTTGCATTTATCTAAAGTATT SRR26075317 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 18:35:14 Started mapping on | Feb 11 18:35:15 Finished on | Feb 11 18:39:07 Mapping speed, Million of reads per hour | 258.61 Number of input reads | 16665668 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 14875528 Uniquely mapped reads % | 89.26% Average mapped length | 291.17 Number of splices: Total | 13004124 Number of splices: Annotated (sjdb) | 12656707 Number of splices: GT/AG | 12759859 Number of splices: GC/AG | 182134 Number of splices: AT/AC | 13642 Number of splices: Non-canonical | 48489 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 2.99 Insertion rate per base | 0.02% Insertion average length | 2.48 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 495779 % of reads mapped to multiple loci | 2.97% Number of reads mapped to too many loci | 45293 % of reads mapped to too many loci | 0.27% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.28% % of reads unmapped: other | 0.22% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1294361 1294361 1294361 N_multimapping 495779 495779 495779 N_noFeature 450936 14712327 548540 N_ambiguous 166837 918 100615 UnstrandedReadsAssigned:14257755 PositiveStrandReadsAssigned:162283 NegativeStrandReadsAssigned:14226373 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075317 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075317-trimmed-pair1.fastq SRR26075317-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,665,668 reads, 14,404,679 reads pseudoaligned [quant] estimated average fragment length: 207.736 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,124 rounds 52401 SRR26075317.ke.tsv 34699 SRR26075317.se.tsv 87100 total ==> SRR26075317.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1811.26 1252 42.4392 Potri.005G024800.1.v4.1 1035 828.264 2141 158.706 Potri.004G059700.1.v4.1 961 754.274 0 0 Potri.007G009000.2.v4.1 1416 1209.26 0 0 Potri.003G141000.2.v4.1 2943 2736.26 616.086 13.8238 Potri.016G087400.1.v4.1 270 93.3015 901 592.9 Potri.015G069301.1.v4.1 564 359.004 0 0 Potri.010G195200.1.v4.1 1773 1566.26 538.959 21.1269 Potri.012G127500.1.v4.1 977 770.264 5621 448.042 ==> SRR26075317.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 24 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 165 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 8 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1238 SRR26075317 completed mapping pipeline successfully