Starting /dee2/code/volunteer_pipeline.sh SRR26075318 current disk space = 3053356142592 free memory = 1475943652 SRR26075318 SRAfilesize 0c9f180215f343bfb4541f6e71744b5a SRR26075318.sra SRR26075318.sra file validated SRR26075318 is paired end SRR26075318 is conventional basespace SRR26075318 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075318_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.65325 37.0 37.0 37.0 37.0 37.0 2 36.7145 37.0 37.0 37.0 37.0 37.0 3 36.7065 37.0 37.0 37.0 37.0 37.0 4 36.681 37.0 37.0 37.0 37.0 37.0 5 36.693 37.0 37.0 37.0 37.0 37.0 6 36.705 37.0 37.0 37.0 37.0 37.0 7 36.69 37.0 37.0 37.0 37.0 37.0 8 36.6735 37.0 37.0 37.0 37.0 37.0 9 36.6315 37.0 37.0 37.0 37.0 37.0 10-14 36.65435 37.0 37.0 37.0 37.0 37.0 15-19 36.62 37.0 37.0 37.0 37.0 37.0 20-24 36.582800000000006 37.0 37.0 37.0 37.0 37.0 25-29 36.461499999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.4314 37.0 37.0 37.0 37.0 37.0 35-39 36.394999999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.3339 37.0 37.0 37.0 37.0 37.0 45-49 36.291399999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.3074 37.0 37.0 37.0 37.0 37.0 55-59 36.2805 37.0 37.0 37.0 37.0 37.0 60-64 36.2402 37.0 37.0 37.0 37.0 37.0 65-69 36.133399999999995 37.0 37.0 37.0 37.0 37.0 70-74 36.1181 37.0 37.0 37.0 37.0 37.0 75-79 36.080600000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.9708 37.0 37.0 37.0 37.0 37.0 85-89 35.8996 37.0 37.0 37.0 37.0 37.0 90-94 35.875800000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.866 37.0 37.0 37.0 37.0 37.0 100-104 35.840500000000006 37.0 37.0 37.0 37.0 37.0 105-109 35.809799999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.6168 37.0 37.0 37.0 37.0 37.0 115-119 35.5878 37.0 37.0 37.0 37.0 37.0 120-124 35.629200000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.520500000000006 37.0 37.0 37.0 37.0 37.0 130-134 35.3298 37.0 37.0 37.0 34.6 37.0 135-139 35.2684 37.0 37.0 37.0 32.2 37.0 140-144 35.0741 37.0 37.0 37.0 27.4 37.0 145-149 35.0972 37.0 37.0 37.0 29.8 37.0 150-151 34.8735 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 1.0 20 1.0 21 1.0 22 3.0 23 3.0 24 5.0 25 8.0 26 5.0 27 13.0 28 19.0 29 28.0 30 43.0 31 39.0 32 66.0 33 77.0 34 131.0 35 453.0 36 2873.0 37 229.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.171722236149414 14.239157683629983 7.821509150162948 42.76761093005766 2 20.225 13.775 35.15 30.85 3 17.9 17.575 25.825 38.7 4 21.925 24.2 22.900000000000002 30.975 5 24.075 31.85 24.45 19.625 6 23.05 31.275 23.799999999999997 21.875 7 14.35 29.599999999999998 39.15 16.900000000000002 8 17.299999999999997 27.800000000000004 30.975 23.925 9 17.224999999999998 23.325000000000003 34.55 24.9 10-14 20.046002300115006 29.076453822691136 27.28636431821591 23.59117955897795 15-19 19.869999999999997 27.93 28.265 23.935000000000002 20-24 20.02 26.985 28.52 24.474999999999998 25-29 19.919999999999998 27.675 28.449999999999996 23.955000000000002 30-34 19.134999999999998 28.975 27.47 24.42 35-39 19.75 28.83 27.815 23.605 40-44 20.315 28.365000000000002 27.029999999999998 24.29 45-49 20.25 28.244999999999997 26.91 24.595 50-54 20.445 28.205000000000002 27.650000000000002 23.7 55-59 20.119999999999997 27.985 27.615000000000002 24.279999999999998 60-64 19.77 26.87 28.884999999999998 24.474999999999998 65-69 20.145 28.37 27.615000000000002 23.87 70-74 20.979999999999997 27.46 27.72 23.84 75-79 20.385 28.060000000000002 27.505000000000003 24.05 80-84 20.765 28.16 27.615000000000002 23.46 85-89 20.82 27.93 27.295 23.955000000000002 90-94 20.48 28.38 26.895000000000003 24.245 95-99 20.46 26.91 28.749999999999996 23.880000000000003 100-104 20.645 27.805000000000003 28.365000000000002 23.185 105-109 19.74 28.660000000000004 27.02 24.58 110-114 20.895 27.79 27.0 24.315 115-119 21.099999999999998 28.32 26.495 24.085 120-124 21.029999999999998 28.000000000000004 26.729999999999997 24.240000000000002 125-129 21.279999999999998 27.744999999999997 26.490000000000002 24.485 130-134 20.86 28.835 25.785000000000004 24.52 135-139 21.59 27.229999999999997 27.16 24.02 140-144 21.065 27.42 26.715 24.8 145-149 21.37 27.755000000000003 26.279999999999998 24.595 150-151 21.5375 26.8125 26.75 24.9 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 2.0 20 2.0 21 0.0 22 0.0 23 0.0 24 1.0 25 2.5 26 3.0 27 3.0 28 6.0 29 8.0 30 16.0 31 25.0 32 30.5 33 26.0 34 33.5 35 66.5 36 76.0 37 84.0 38 120.5 39 155.5 40 165.0 41 201.5 42 256.0 43 269.0 44 278.0 45 294.0 46 286.0 47 268.5 48 239.5 49 196.0 50 173.5 51 140.0 52 122.0 53 106.5 54 74.0 55 68.0 56 51.5 57 35.5 58 26.5 59 20.5 60 16.0 61 11.5 62 10.0 63 4.5 64 6.0 65 5.0 66 1.5 67 2.0 68 3.5 69 2.5 70 0.0 71 0.5 72 1.5 73 1.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27499999999999997 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.275000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 64.95383380168607 40.45 2 20.11240465676435 25.05 3 8.751505419510238 16.35 4 3.5327177840224806 8.799999999999999 5 1.5656362906463268 4.875 6 0.7627458851866721 2.85 7 0.08028904054596547 0.35000000000000003 8 0.16057808109193095 0.8 9 0.04014452027298274 0.22499999999999998 >10 0.04014452027298274 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCAGAACATTGAAAGTCTCCGCATCAGCACAAGTTAAGATGGAAGTGTCA 10 0.25 No Hit CCAAAAAGTTTACCAGTGAGCCAGTTGCCAAAATCACGTTGCCATGCTGA 9 0.22499999999999998 No Hit GGCCCCTCATGGTCCATAATGCCCGCAGAAGTTGTCAAGACAATATATCC 8 0.2 No Hit CTGAATGTTGTAGTCGGCAAGGGTACGGCCATCCTCAAGCTGCTTGCCAG 8 0.2 No Hit CACACTTTGCCCGGAAGCTTCGCACCAATTCCTACCCAATTACCCTACAT 8 0.2 No Hit GTCATGCCCTCCACTTTGGCCTAGGAAAACCTGAATCATTCCAGGGTGAT 8 0.2 No Hit GTAGTACTCGTCACTTCTACTATCACCCTTCCTGTTCTGGAAAGGCTTCC 7 0.17500000000000002 No Hit CTAGTTTCTCCTAGTTGGGTGCAGTGCTCATGGAAACTGCTGATAGGTCG 7 0.17500000000000002 No Hit GTCCAAAGTTAAGCTCTGCACCTGTTACACTATAGTCATCGTCCATAACA 6 0.15 No Hit GGCTTGCAATGGAATGGCCCTTAACAGCCACTCCAAACAGTACTAGGTGA 6 0.15 No Hit ACGAAGGGAAACCCATGGACAAAGTTGGTTGTTCCATCAAGAGGATCAAC 6 0.15 No Hit ATTTTAATATCATTAATAGCATGATGGTGATTGTTTTGAAAACCATAGGA 6 0.15 No Hit CGGGCTGGTTTTGATGCCGGAGTTTTGGGTGCACTGCTGCTGGGTTTAGC 6 0.15 No Hit AGAGTGTGCAGGCGAGCCACTGTGTGTCGGAACGCTTGGGATCACTGTCC 6 0.15 No Hit AGAAAACATAGTAAGCACGCTTTTGAGATGCATAAATTGCTCTTCCTGCA 6 0.15 No Hit TATTGGGCTTCGGTGCAACCTGCACAAATCATTAACAACAAATGATTGGC 6 0.15 No Hit CGGCGTTCATTGGCTGGTCTTTTTCATGGCGCAAACTGTTTTCCCGACTG 6 0.15 No Hit GTGCAAATAACCGTGGCTTGAACATGGGATATTTGAGTTGGAGTTACAAT 6 0.15 No Hit TAGTAGAAGCACTTGGAGGTGGCGGTGGCATTTGGGTCTAGACTCGTTAA 6 0.15 No Hit ACCACATTTGCAGTCACTGCCGCACTTGCAGTCAGAGCCACAGCTACAAC 6 0.15 No Hit ATCTGCAAAATCCACATTAACTAGTCCAGGTATAGTAATAATATCTGAAA 6 0.15 No Hit TTCGTGTTTCACAATCCATCTCCAAATACATCTGTTTTTGTTGTGTTTCA 6 0.15 No Hit AGGACCACTCCAGTGTAAAATTGAAAAAGCCATGCCCAGATGATTGCTTC 6 0.15 No Hit CCTGAAGAGGACCTAAAGATCCCTGAAAACCTACTAGCCGACGAGTTCCT 6 0.15 No Hit CATAGGAATACTTGACATCATCAACAGAACACATTTCTCTTATACTGTGC 6 0.15 No Hit CTCTGAATGGCTGAGGGACATGCTGTACTTCCACTGCCTCCTTGTTGCTG 6 0.15 No Hit GGACTAGACGCTCCATTTTGAGTATGCTCAGAAGGTGGAAGCTTCGAAGG 6 0.15 No Hit CCTCATAACTGGTTATAACCAATTTTGGGTCATGAGATGCCCTCTCTACA 5 0.125 No Hit CTCCAATAGATATATCACAAGAATCCTCTTAACCAGATCCATTGCTAAAA 5 0.125 No Hit CGTGGGACGATTAAAGACAAGTAATTTGAAACTGCCTTTATCAGCAATGA 5 0.125 No Hit CCACCTGACCGTCATCTAGTTCGGCCTTGTATACAGTTCCAAAGCCTCCT 5 0.125 No Hit GGCCAGTAATGGTATTGCGTTGCTGCCCAAAATGTCTTAAAAGTTCCATC 5 0.125 No Hit CCCATGCTTGCCCTCGAAGCCTCGCCGTCTTCAAAGCAACAACATCCAAA 5 0.125 No Hit CCTCAGCCTGGCCCTGGCCTTCAACTCCCCCCGAACATCCTGTTCCACCA 5 0.125 No Hit CCTCTGGGTTCATGGTCCAAACAATATTGCTGAATTTGTCTCCAATTGGC 5 0.125 No Hit CTGGAAACCTGAAATTTAACCTCTGACTCTCCCGTAGTTGTTTCAGTGTC 5 0.125 No Hit CCCTACCACCCATGGCATGAAAAAAGAACTCTTGTGGGGTTAACCCACGC 5 0.125 No Hit ATTGCTTCCATATCCGCTTCCGCTTCCATATCCACCCCTTCCACGGCCAC 5 0.125 No Hit AATAAACAAAAGATTGGGGCCCGTTTCTTCACTTTGATCAGACAAGGTCA 5 0.125 No Hit CTCCTGAGAACACAAAATCAAAGGAATTTTCTGCATATTCAAGCTCATAA 5 0.125 No Hit CTTGCATAGCTGAAACCATCTTCTGGGGTGACATGGATTGTGGAAATTGC 5 0.125 No Hit TGTTAGGCCACTGGATGAGGTTGAGGACATCACAGAGAGAACTTCTGTCA 5 0.125 No Hit GCAGTGTCAGAGGAGAGGTTATTGATGGAGGCGATGCGAGTGGCGGATTG 5 0.125 No Hit GTCCACTCGATGTCGGTCTCGAAGGAGGATTTCCATGTTAGAAGGAGCAT 5 0.125 No Hit GTCTTGGCTGAAGATTTAAAACAAACAAAAGACAAAAATTGTCCGAAAAT 5 0.125 No Hit CCACCAACATCACCACTGCGAGACATGTAATCACCGCCATAGCTTGATGA 5 0.125 No Hit CTGTTGCTTTTGCTGTTTAGAGTCTTTATTAAGCAACAAACGACTCATCG 5 0.125 No Hit GCACCTTTACCGCTATTCATTGCTCTGCAAAAGTTGATTCTGTCCCTATG 5 0.125 No Hit CTTATAACTATAAAGCAAAGCCTCCTTTGCAGCATCCTCGCTGCCAAGAA 5 0.125 No Hit GTCGTGAATACCCAGATTAGTGCCCCAGTGTAAGCTCTTCAAGGGTTCAA 5 0.125 No Hit CCCTCATCATCCTTGTATCCAATCTTTTCCAGTATTTCATGTATAGCAGT 5 0.125 No Hit CTCCACTATTATCAGATGCCATGGACCCTTCACGAAAATGGACAAGGATG 5 0.125 No Hit CTCCATTCCATGATCGCTATCACCATCATCGCTATCCTCATCATGGTGAC 5 0.125 No Hit CACATCTGATATCTCCTCTCCAAGGTGTTCTTTCTCTTCATCTTTCCAAT 5 0.125 No Hit GACAGCTTCACCAGATGTCATCATCATTCTTCTACGAACAGTGTCAATGG 5 0.125 No Hit CCAACAAGCAAGATAGCAACTACAAGAACCACCTTATTAGAGCTCGCAAG 5 0.125 No Hit GCTCAGTACTCTTCTGATACTTACGGATTTCACGAAGAGCAACAGTTCCA 5 0.125 No Hit CACCACCACCGGAAGGAACGGAAGCCAACTTTTCCCTGCCGGAAGCAATC 5 0.125 No Hit GGCTAGAACAAGGAGATACAGGCATAGATGTGATCATTCCGCCATCATCC 5 0.125 No Hit CAGTGCTAGAACTAGAAATTCCCTGATAGGGTTCTTCTCCTTTTCTCTCA 5 0.125 No Hit CCCTGGTGATCCTACTTTCACTGCCCTCGCATTTTCAGACCAAAGTAGAA 5 0.125 No Hit CGCGATACAAGAGGGCATATAGTGCCCTGAAATTGCAGCTTTTACTGATT 5 0.125 No Hit CCTTGTCAGAATTGAAATACATAGAAGCATTGTCAGAAGGAGTCAAGGGT 5 0.125 No Hit CTTCACATCAAGACCCCGTGCAGCAACATCAGTGGCAGTCATTATCGCAC 5 0.125 No Hit CTCTTTCTCTCAGCCTCTATCTATACACAAGGTGGATCTTGAAACTGTGA 5 0.125 No Hit GGTAGTACTTTCAGTTCATTGACATTGTTATGCTTCCATCATGATTACTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.025 0.0 0.0 0.0 60-61 0.0 0.025 0.0 0.0 0.0 62-63 0.0 0.025 0.0 0.0 0.0 64-65 0.0125 0.025 0.0 0.0 0.0 66-67 0.11249999999999999 0.025 0.0 0.0 0.0 68-69 0.175 0.025 0.0 0.0 0.0 70-71 0.175 0.025 0.0 0.0 0.0 72-73 0.175 0.025 0.0 0.0 0.0 74-75 0.1875 0.025 0.0 0.0 0.0 76-77 0.25 0.025 0.0 0.0 0.0 78-79 0.2875 0.025 0.0 0.0 0.0 80-81 0.3125 0.025 0.0 0.0 0.0 82-83 0.375 0.025 0.0 0.0 0.0 84-85 0.475 0.025 0.0 0.0 0.0 86-87 0.525 0.025 0.0 0.0 0.0 88-89 0.7125 0.025 0.0 0.0 0.0 90-91 0.85 0.025 0.0 0.0 0.0 92-93 0.9874999999999999 0.025 0.0 0.0 0.0 94-95 1.15 0.025 0.0 0.0 0.0 96-97 1.35 0.025 0.0 0.0 0.0 98-99 1.525 0.025 0.0 0.0 0.0 100-101 1.875 0.025 0.0 0.0 0.0 102-103 2.45 0.025 0.0 0.0 0.0 104-105 2.7625 0.025 0.0 0.0 0.0 106-107 3.3375 0.025 0.0 0.0 0.0 108-109 3.6500000000000004 0.025 0.0 0.0 0.0 110-111 4.1375 0.025 0.0 0.0 0.0 112-113 4.575 0.025 0.0 0.0 0.0 114-115 4.987500000000001 0.025 0.0 0.0 0.0 116-117 5.6125 0.025 0.0 0.0 0.0 118-119 6.362500000000001 0.025 0.0 0.0 0.0 120-121 7.262499999999999 0.025 0.0 0.0 0.0 122-123 8.0 0.025 0.0 0.0 0.0 124-125 8.6875 0.025 0.0 0.0 0.0 126-127 9.275 0.025 0.0 0.0 0.0 128-129 9.85 0.025 0.0 0.0 0.0 130-131 10.6125 0.025 0.0 0.0 0.0 132-133 11.375 0.025 0.0 0.0 0.0 134-135 12.1125 0.025 0.0 0.0 0.0 136-137 13.5 0.025 0.0 0.0 0.0 138-139 14.537500000000001 0.025 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCATATG 10 0.006830828 145.0 3 GTCACCA 40 0.0076550315 18.125 135-139 >>END_MODULE SRR26075318 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075318_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.423 37.0 37.0 37.0 37.0 37.0 2 36.456 37.0 37.0 37.0 37.0 37.0 3 36.3915 37.0 37.0 37.0 37.0 37.0 4 36.4655 37.0 37.0 37.0 37.0 37.0 5 36.4845 37.0 37.0 37.0 37.0 37.0 6 36.4905 37.0 37.0 37.0 37.0 37.0 7 36.499 37.0 37.0 37.0 37.0 37.0 8 36.5345 37.0 37.0 37.0 37.0 37.0 9 36.506 37.0 37.0 37.0 37.0 37.0 10-14 36.543 37.0 37.0 37.0 37.0 37.0 15-19 36.4677 37.0 37.0 37.0 37.0 37.0 20-24 36.471000000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.3752 37.0 37.0 37.0 37.0 37.0 30-34 36.268600000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.2538 37.0 37.0 37.0 37.0 37.0 40-44 36.212300000000006 37.0 37.0 37.0 37.0 37.0 45-49 36.144999999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.1021 37.0 37.0 37.0 37.0 37.0 55-59 36.0792 37.0 37.0 37.0 37.0 37.0 60-64 36.1091 37.0 37.0 37.0 37.0 37.0 65-69 36.0794 37.0 37.0 37.0 37.0 37.0 70-74 35.9705 37.0 37.0 37.0 37.0 37.0 75-79 36.0033 37.0 37.0 37.0 37.0 37.0 80-84 35.9646 37.0 37.0 37.0 37.0 37.0 85-89 35.9029 37.0 37.0 37.0 37.0 37.0 90-94 35.8577 37.0 37.0 37.0 37.0 37.0 95-99 35.9097 37.0 37.0 37.0 37.0 37.0 100-104 35.8155 37.0 37.0 37.0 37.0 37.0 105-109 35.7989 37.0 37.0 37.0 37.0 37.0 110-114 35.717699999999994 37.0 37.0 37.0 37.0 37.0 115-119 35.659800000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.531099999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.5086 37.0 37.0 37.0 37.0 37.0 130-134 35.4412 37.0 37.0 37.0 34.6 37.0 135-139 35.3399 37.0 37.0 37.0 32.2 37.0 140-144 35.305600000000005 37.0 37.0 37.0 29.8 37.0 145-149 35.360299999999995 37.0 37.0 37.0 34.6 37.0 150-151 35.0175 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 4.0 15 1.0 16 2.0 17 0.0 18 1.0 19 0.0 20 1.0 21 2.0 22 5.0 23 8.0 24 3.0 25 9.0 26 5.0 27 7.0 28 10.0 29 14.0 30 20.0 31 39.0 32 37.0 33 82.0 34 198.0 35 627.0 36 2714.0 37 211.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.15 20.75 13.375 29.725 2 28.65 25.525 26.724999999999998 19.1 3 23.05 27.025 30.925000000000004 19.0 4 25.275 31.900000000000002 21.625 21.2 5 25.825 34.599999999999994 22.875 16.7 6 22.35 37.15 22.15 18.35 7 21.0 21.15 37.875 19.975 8 22.650000000000002 25.15 29.725 22.475 9 23.65 23.575 28.125 24.65 10-14 23.21 29.65 25.91 21.23 15-19 24.154999999999998 27.615000000000002 27.165 21.065 20-24 23.9 27.88 27.245 20.974999999999998 25-29 23.275000000000002 28.98 27.634999999999998 20.11 30-34 23.365 28.395 26.590000000000003 21.65 35-39 23.73 28.77 26.865 20.635 40-44 24.21 28.360000000000003 28.050000000000004 19.38 45-49 24.45 28.1 26.515 20.935000000000002 50-54 23.880000000000003 27.88 27.894999999999996 20.345 55-59 24.595 27.71 26.88 20.815 60-64 23.73 27.295 27.944999999999997 21.029999999999998 65-69 24.435000000000002 28.1 27.095000000000002 20.369999999999997 70-74 24.154999999999998 27.474999999999998 27.839999999999996 20.53 75-79 23.955000000000002 27.96 27.325 20.76 80-84 23.53 27.87 27.3 21.3 85-89 24.11 28.7 26.615 20.575 90-94 23.35 28.615000000000002 27.279999999999998 20.755000000000003 95-99 24.5 27.750000000000004 27.785 19.965 100-104 24.015 27.975 27.61 20.4 105-109 25.009999999999998 28.535 26.93 19.525000000000002 110-114 24.834999999999997 27.87 27.0 20.294999999999998 115-119 24.64 29.005 26.224999999999998 20.13 120-124 25.105 28.815 26.32 19.759999999999998 125-129 26.355 28.144999999999996 26.284999999999997 19.215 130-134 25.765 28.134999999999998 26.565 19.535 135-139 25.424999999999997 28.67 26.58 19.325 140-144 26.674999999999997 28.139999999999997 26.424999999999997 18.759999999999998 145-149 26.44 28.835 25.44 19.285 150-151 28.0625 26.137500000000003 26.487500000000004 19.3125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 1.0 17 1.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.5 25 1.5 26 2.5 27 3.0 28 4.5 29 8.0 30 7.0 31 6.0 32 13.0 33 19.5 34 28.0 35 47.5 36 69.5 37 99.0 38 132.5 39 184.0 40 210.5 41 219.5 42 251.0 43 259.0 44 277.0 45 281.0 46 290.5 47 281.0 48 230.0 49 212.5 50 182.0 51 125.5 52 100.5 53 101.0 54 83.0 55 66.0 56 51.5 57 32.0 58 22.5 59 18.0 60 14.5 61 11.5 62 9.0 63 6.5 64 4.5 65 4.5 66 3.5 67 2.0 68 4.0 69 3.5 70 0.5 71 2.0 72 2.0 73 0.5 74 0.5 75 0.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.5 81 0.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.625 #Duplication Level Percentage of deduplicated Percentage of total 1 65.86826347305389 41.25 2 19.680638722554892 24.65 3 8.223552894211576 15.45 4 3.273453093812375 8.200000000000001 5 1.7564870259481038 5.5 6 0.7984031936127743 3.0 7 0.1596806387225549 0.7000000000000001 8 0.19960079840319359 1.0 9 0.0 0.0 >10 0.03992015968063872 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCACATCTTCCACCTCTACACCCAAGATCCAGGGCTTCTCATAACAATC 10 0.25 No Hit GTTAGGCTTGAGCAGATTCATTCGCCAACTAACCCTTTAATTTATCCTAT 8 0.2 No Hit CACATGAAGTTCAATGAGCTTTGGTCCGACGCTCCTTGGGATTTAATGTA 8 0.2 No Hit CCCGAGTTCTATTTTGCTCAAAAGATAGATTATCTCAAAGATAAGGTTGA 8 0.2 No Hit AATCTCTCTCTCTCTCTCTCTCTCTCTTCCTCTTCCTCTTTTTTTTTCCT 8 0.2 No Hit AAGCATTCAACTCTTTCAAGATGCAAATCTTCGTCAAGACTCTCACTGGC 8 0.2 No Hit AGAAAATATGGGCAGAAAGATATTCTAGGAGCAAAATATCCTAGAAGCTA 7 0.17500000000000002 No Hit AGGGCTGGTAAAATTGTGGTTGAATTGAATGGAAGATTGAACAAATGTGG 7 0.17500000000000002 No Hit GTTAGAGCAGATGGTGGATGGTTCAATGACATATTGAATACAGTTTTCTC 7 0.17500000000000002 No Hit GATAAGGGGATTTACACTGTGGCAGAGACCATGTCAGAGAGTGACCGTTG 7 0.17500000000000002 No Hit ATTGAACGTACCTTATCACGTGTTTGCCTGGAAGTACTTATAGACCCTAG 6 0.15 No Hit GCCAGAGAGAGAGCAATGGATCAATCATTCAGTGGAGGAGGGAGCTGGAC 6 0.15 No Hit GTGTCATTCAGCTTTTATATCCCCTATTCTCTTCGTGAAGATGTCTTGCT 6 0.15 No Hit TAGCAAATGTAGGAGAGAGAGGTTAATAAGGCCTAGCCTAAAGGTTCTTG 6 0.15 No Hit ACCAACCCTTTTAGCCGTCCTTGTAATCCTTATCTTCAATATAACTTCTT 6 0.15 No Hit GGGGACTCGGCACTATCCGAAGGATACTTCTGGGGAGTGTGAGCACTTAT 6 0.15 No Hit CGGCTATTTCCATCATTCCTTCGTTGCAGATCTTGTCTGGGTCTGATCCT 6 0.15 No Hit CCCTGGCAGACTACAATATCCAGAAGGAGTCCACACTTCACCTTGTCCTT 6 0.15 No Hit CAGGTGGCCATGTTGCATCTCCAGATGAAGAGGAGAAGGCTGCTAGACGC 6 0.15 No Hit GGATATTTGATCCCTAAGGGTTGGAAAGTCTTGCCACTTTTCAGAAACAT 6 0.15 No Hit ATATTACTAGTTTAGCTCCGTCTTCACCTTCAAGGTCTCCAAAACGTCCT 6 0.15 No Hit GTGTTTGTGCAACCTCTTAATTCGACAGGGTACCGAAGAAGAATGGCATC 6 0.15 No Hit GGAGTCTACATCTTTGTCAAATTGGCAAGGACAATCAACTGATGGTGCTT 6 0.15 No Hit GCATGTGGAGCATAAAGGCCTGGTGGATTTAGTTACTGAGACTGACAAGG 6 0.15 No Hit AATCGTTACCACACAGGATTGGATTGAGAAGGTTGGTCCAATGACCCCAT 6 0.15 No Hit GTACGGGATCCGGTGCTGCCCCAGTTGTAGCTCAGATATCAAAGGAGGCA 6 0.15 No Hit TATCGGTCCAATCCTAGCTAGTGGTGTTGGGATTCGTACTGTTGACGTTG 6 0.15 No Hit GAGTGATGCTAATTATTGTTGGCTTCCATTCACATCACCCAGAATTGAAT 6 0.15 No Hit TTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGG 6 0.15 No Hit CCCTAGTCCCTCTAGATTCTCTCAGTCTCCAAAACTCAGTAGATTAGGAT 6 0.15 No Hit GGAGGCACCACTAGAGGCTGCATCAGCTTGCCCTGGCGTTTATGGCAAGG 5 0.125 No Hit CCCAAGTAAAAATGTCTCAGCCTGAAAAACGCACTACTAAAAGAAGTCAT 5 0.125 No Hit ATAGAAAGCAAAGAAACATAGGGACAGAATCAACTTTTGCAGAGCAATGA 5 0.125 No Hit GGAAAACTCACAGAAACGACTTACGAAGGCAAATTTAAGCCAGGATTTTA 5 0.125 No Hit ATTTGCTGCTATAATGACCACAAGACAGTTGGTGAGCATTGTGCTATCGT 5 0.125 No Hit CAGGGGCTGGGGTTACCCTAGAAGAGCTCAAGAAGAGATTGGCTGAGTTT 5 0.125 No Hit GTGGCATATGTGATGGGAAGTCCCAACTCAACTCAGAAATGGCATGTTTA 5 0.125 No Hit CAAATCAATGACTTGGGTGATGGAGAACAAGAGCTCAACACCAGGGAATA 5 0.125 No Hit AACATGGCATCTTATGATGCAGCAGTTCTTGACGGGAAACTTCTTGTGAC 5 0.125 No Hit CAAACTCTAACAATTGTTACTTCTCTCTCTCTCTCTCTCACTTTTTGGGT 5 0.125 No Hit GTGGGAAGTCACAAGTCCGGGAGCTTGCAGGATTTAAAACAACTGGGTGG 5 0.125 No Hit ATTGGTTTCCGAGAAAATAACAGTAGTGAGCCTAAGCATGGGAAAGTTGT 5 0.125 No Hit GCTAAGCGCACAAATTAAGGCTTAAAGATATAGAGAGAAAGAAACAACAT 5 0.125 No Hit AGCGGATAGAAAACGCCTTTTCAACATGATAAATGATCTTCCAACAGTGT 5 0.125 No Hit AACCGAACAAGTGCCAATTCTTGGGATGGAGATTTTGCAATGAAGCCAGG 5 0.125 No Hit GAGTAAATGCAGGAAGATTTTTGATGGAAGATCTGTACAGAAATCCAGGT 5 0.125 No Hit CAGAACCAAAAAGGATAAGGGATGAGGTTGATGTAGATGATGATCAACGC 5 0.125 No Hit CCTCAAACAGCTATGCCATTAAGAAGAAAGATGAGATTGAAAGAGTTGCT 5 0.125 No Hit TTCTGCTCTAGAAGGCCATTTTTCAAAAGATGATGGAACAGAGTTGGTTA 5 0.125 No Hit CAGGGAGGGAGCGAGAGATTCTCGAAGAGATTCAAAGATGGCCCGTACCA 5 0.125 No Hit TGTCTATCGTGGTCTCTACTTTGGAATGTACGACTCCTTGAAGCCAGTGC 5 0.125 No Hit GTTTCTGCATGTGTAAGTGGCATGGATCTTCATGCATTGAGTGCTTGCCT 5 0.125 No Hit GGCTTCTTGTCCTAAATCCCAGTACTATATACCAGAATTGGTTTGGTGCC 5 0.125 No Hit CTCTTGTCCCAAGAGCAAAACCTACGATGAGTTTTAGCACTTCACATCTC 5 0.125 No Hit TGACTACTCGATGATGGTAACCACTGATATGATGACCCCAATCTCATTTG 5 0.125 No Hit CATTGCAACGCATTCATTCTGCAAAGTGAGCAAGATGGTAACTTTTCTTG 5 0.125 No Hit AGAAGCTGCTTGTGCTGCTCTTTCAAACCTTTTCCTGCCCTTCGCTAGCT 5 0.125 No Hit TGCCAAGGTTGCTAGGGTCCAGCCTCCTGTTGAGAATTTGGACGTGCTGA 5 0.125 No Hit CCGAAACTCTAAGATCAGAAGAAAGATTATACAGAAATTAGTGCCCAAGA 5 0.125 No Hit CTGCTGAGGAGAAATCCTTACTTCTTAATAAGATTCAAGATATAAATGAA 5 0.125 No Hit CGATTAAGGGAAAGTGAAGCGGAAGTCCAGAGGGCCACGCGCAAAAACGC 5 0.125 No Hit AAGGATTGAATCCCAGCATAAAATGAAAGCAGAAAAACAGCAAAAGAGAT 5 0.125 No Hit AGGGATTTCTGTGCAACGGAGTTCAATCATCATGCCAATCCTACCTCACC 5 0.125 No Hit GCAATGGTCACTGCTGGATCGAAGGGGAGTTTCATTAACATATCACAGAT 5 0.125 No Hit AGCCAGATGCCTCACCTTCATCTGCCACTTAGCTGAACCAGTATATCCTG 5 0.125 No Hit CGTTAGTTTGCAGTTTACGGTGGGTCGTTTTACTAGGTTCTTGAAGGCAG 5 0.125 No Hit CCCCATTTCTTCCATCTCTCTTACATTTTTTGTCAATGCTTTTCTTCTTT 5 0.125 No Hit CGGCGGGGATCCCGAAGATTAAACTACAAAGAATGGCCGAGTCTGTTCCT 5 0.125 No Hit CCTCCCCTCTCCCTCTTCTCTCTCTCAAAAACCCCCCAAAATCTGTCTCA 5 0.125 No Hit TTCTCATCAATCCAGTCCGTCTTTGTCTTCACGTGTAAACTATATATAGC 5 0.125 No Hit TGGAAGTCGGATTCTGATTTTCATGGAGACAAAAAAAGGCCGTGACCAAG 5 0.125 No Hit GCCGGTGGTAATGGTGGGAAGTATTACTTGGGGATGTTTTTGGGCGGGGC 5 0.125 No Hit GATTCAGAAACATGTCCTCAGGGCCAAGGCAAGAGAGATGGGACCTATCA 5 0.125 No Hit GCGATTGGCCCAAGTGGCCGGACCATTACCAAGGAAGCTCGGATGCACTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0125 0.0 0.0 0.0 0.0 66-67 0.11249999999999999 0.0 0.0 0.0 0.0 68-69 0.175 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.225 0.0 0.0 0.0 0.0 78-79 0.2625 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.35 0.0 0.0 0.0 0.0 84-85 0.45 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.6875 0.0 0.0 0.0 0.0 90-91 0.825 0.0 0.0 0.0 0.0 92-93 0.9625 0.0 0.0 0.0 0.0 94-95 1.125 0.0 0.0 0.0 0.0 96-97 1.3250000000000002 0.0 0.0 0.0 0.0 98-99 1.5125000000000002 0.0 0.0 0.0 0.0 100-101 1.8875 0.0 0.0 0.0 0.0 102-103 2.475 0.0 0.0 0.0 0.0 104-105 2.7750000000000004 0.0 0.0 0.0 0.0 106-107 3.325 0.0 0.0 0.0 0.0 108-109 3.6375 0.0 0.0 0.0 0.0 110-111 4.1375 0.0 0.0 0.0 0.0 112-113 4.575 0.0 0.0 0.0 0.0 114-115 5.0 0.0 0.0 0.0 0.0 116-117 5.625 0.0 0.0 0.0 0.0 118-119 6.3375 0.0 0.0 0.0 0.0 120-121 7.25 0.0 0.0 0.0 0.0 122-123 8.025 0.0 0.0 0.0 0.0 124-125 8.7625 0.0 0.0 0.0 0.0 126-127 9.3125 0.0 0.0 0.0 0.0 128-129 9.875 0.0 0.0 0.0 0.0 130-131 10.5625 0.0 0.0 0.0 0.0 132-133 11.35 0.0 0.0 0.0 0.0 134-135 12.0875 0.0 0.0 0.0 0.0 136-137 13.475000000000001 0.0 0.0 0.0 0.0 138-139 14.5 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTGTA 35 0.0033124194 62.14286 145 >>END_MODULE Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462674 spots for SRR26075318.sra Written 2462674 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra Read 2462662 spots for SRR26075318.sra Written 2462662 spots for SRR26075318.sra SRR ids: ['SRR26075318.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_terr5ks2 SRR26075318.sra spots: 49253252 blocks: [[1, 2462662], [2462663, 4925324], [4925325, 7387986], [7387987, 9850648], [9850649, 12313310], [12313311, 14775972], [14775973, 17238634], [17238635, 19701296], [19701297, 22163958], [22163959, 24626620], [24626621, 27089282], [27089283, 29551944], [29551945, 32014606], [32014607, 34477268], [34477269, 36939930], [36939931, 39402592], [39402593, 41865254], [41865255, 44327916], [44327917, 46790578], [46790579, 49253252]] SRR26075318 file size 18192918 SRR26075318 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075318 SRR26075318_1.fastq SRR26075318_2.fastq Input file: SRR26075318_1.fastq Paired file: SRR26075318_2.fastq trimmed: SRR26075318-trimmed-pair1.fastq, SRR26075318-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 19:22:24 2025 >> started Tue Feb 11 19:23:19 2025 >> done (54.857s) 49253252 read pairs processed; of these: 273 ( 0.00%) short read pairs filtered out after trimming by size control 2312 ( 0.00%) empty read pairs filtered out after trimming by size control 49250667 (99.99%) read pairs available; of these: 10086395 (20.48%) trimmed read pairs available after processing 39164272 (79.52%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 10 0.00% 19 11 0.00% 20 14 0.00% 21 23 0.00% 22 11 0.00% 23 33 0.00% 24 30 0.00% 25 21 0.00% 26 22 0.00% 27 30 0.00% 28 29 0.00% 29 21 0.00% 30 25 0.00% 31 35 0.00% 32 31 0.00% 33 40 0.00% 34 27 0.00% 35 35 0.00% 36 33 0.00% 37 31 0.00% 38 38 0.00% 39 28 0.00% 40 52 0.00% 41 51 0.00% 42 61 0.00% 43 60 0.00% 44 89 0.00% 45 76 0.00% 46 101 0.00% 47 105 0.00% 48 103 0.00% 49 145 0.00% 50 181 0.00% 51 239 0.00% 52 258 0.00% 53 262 0.00% 54 255 0.00% 55 370 0.00% 56 414 0.00% 57 500 0.00% 58 558 0.00% 59 699 0.00% 60 860 0.00% 61 933 0.00% 62 1227 0.00% 63 1464 0.00% 64 1638 0.00% 65 1923 0.00% 66 2141 0.00% 67 2386 0.00% 68 2983 0.01% 69 3355 0.01% 70 4271 0.01% 71 4972 0.01% 72 6024 0.01% 73 7054 0.01% 74 7886 0.02% 75 8957 0.02% 76 10215 0.02% 77 11091 0.02% 78 12184 0.02% 79 14108 0.03% 80 16106 0.03% 81 17979 0.04% 82 20811 0.04% 83 23829 0.05% 84 27046 0.05% 85 29588 0.06% 86 31714 0.06% 87 34811 0.07% 88 37474 0.08% 89 39231 0.08% 90 41745 0.08% 91 45959 0.09% 92 49616 0.10% 93 53818 0.11% 94 59339 0.12% 95 63553 0.13% 96 68082 0.14% 97 71303 0.14% 98 72943 0.15% 99 77028 0.16% 100 80439 0.16% 101 83363 0.17% 102 87435 0.18% 103 92990 0.19% 104 99160 0.20% 105 103865 0.21% 106 109597 0.22% 107 113428 0.23% 108 115434 0.23% 109 118505 0.24% 110 120633 0.24% 111 122487 0.25% 112 127683 0.26% 113 131710 0.27% 114 137306 0.28% 115 144159 0.29% 116 151083 0.31% 117 153709 0.31% 118 156994 0.32% 119 160413 0.33% 120 161626 0.33% 121 165364 0.34% 122 165383 0.34% 123 170328 0.35% 124 177420 0.36% 125 180683 0.37% 126 188074 0.38% 127 193780 0.39% 128 197027 0.40% 129 200021 0.41% 130 201727 0.41% 131 201199 0.41% 132 204372 0.41% 133 207382 0.42% 134 209365 0.43% 135 214192 0.43% 136 222139 0.45% 137 225367 0.46% 138 231863 0.47% 139 236022 0.48% 140 235047 0.48% 141 237847 0.48% 142 239291 0.49% 143 239335 0.49% 144 242897 0.49% 145 245927 0.50% 146 249721 0.51% 147 253754 0.52% 148 260474 0.53% 149 260622 0.53% 150 264949 0.54% 151 39164272 79.52% 49250667 reads passed initial QC criterion=sequence-density sequence-density=0.18 sequence-density-rank=1 fanout-score=4.80 fanout-score-rank=26 prefix-density=0.25 prefix-fanout=3.4 sequence=TCCACACTTGCAGCCATTCTCAGCACC criterion=fanout-score sequence-density=0.05 sequence-density-rank=35 fanout-score=86.67 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=16.1 sequence=CCTTCTCAAATTAAAGTGGAACAAGTTGAAGTTGAAGGGTCAAATAAAGCTGGAAGCACGTATTTCCTTCCATTAACACCATGAGCATTATAGCTAGCACCAGTTGTAGAGTCCACTAACAAATCCCCAGCATAACCAGGATACGCACCCTTACCATAGACCCCAGGACAAGCAGATGCGGCCTCAAGAGGAGCCTCCTTTGGACCCTGGAAATAGCCATTTTCAAATGGGTTTGTTGCAGTCCCAGCCAAAAGACTAGCCAGATTGATTACCATACCATC criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=2.44 fanout-score-rank=29 prefix-density=0.34 prefix-fanout=2.4 sequence=ATGTACCCTGACTTAGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=110.06 fanout-score-rank=1 prefix-density=0.16 prefix-fanout=5.3 sequence=TCCTGCTCTCGCAATCGCTGCTTCTTTGTCTGTCTTTGGGTCGATCCGAAAGAGAGGAGCTCTTCTGCGCAATCATGTTGGTCTATCAAGATCTTCTCTCTGGTGATGAGCTTCTCTCGGATTCGTTCCCATACAAGGAGATTGAGAATGGGATACTGTGGGAAGTTGAAGGAAAGTGGGTTGTTCAAGGAGCCGTTGATGTAGACATTGGTGCAAATCCTTCAGCTGAAGGAGGTGATGAGGAT SRR26075318 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 19:24:01 Started mapping on | Feb 11 19:24:02 Finished on | Feb 11 19:31:46 Mapping speed, Million of reads per hour | 382.12 Number of input reads | 49250667 Average input read length | 291 UNIQUE READS: Uniquely mapped reads number | 43876541 Uniquely mapped reads % | 89.09% Average mapped length | 289.91 Number of splices: Total | 37530639 Number of splices: Annotated (sjdb) | 36569046 Number of splices: GT/AG | 36859311 Number of splices: GC/AG | 496566 Number of splices: AT/AC | 37540 Number of splices: Non-canonical | 137222 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 3.00 Insertion rate per base | 0.02% Insertion average length | 2.57 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1644006 % of reads mapped to multiple loci | 3.34% Number of reads mapped to too many loci | 120353 % of reads mapped to too many loci | 0.24% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.18% % of reads unmapped: other | 0.15% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3730120 3730120 3730120 N_multimapping 1644006 1644006 1644006 N_noFeature 1201593 43384077 1479553 N_ambiguous 506277 2789 290191 UnstrandedReadsAssigned:42168671 PositiveStrandReadsAssigned:489675 NegativeStrandReadsAssigned:42106797 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=148 echo kmer=143 SRR26075318 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075318-trimmed-pair1.fastq SRR26075318-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 49,250,667 reads, 42,972,339 reads pseudoaligned [quant] estimated average fragment length: 197.89 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,136 rounds 52401 SRR26075318.ke.tsv 34699 SRR26075318.se.tsv 87100 total ==> SRR26075318.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1821.11 5930 65.4031 Potri.005G024800.1.v4.1 1035 838.11 8815 211.252 Potri.004G059700.1.v4.1 961 764.115 28 0.736002 Potri.007G009000.2.v4.1 1416 1219.11 0 0 Potri.003G141000.2.v4.1 2943 2746.11 2354 17.2174 Potri.016G087400.1.v4.1 270 95.3648 3679 774.857 Potri.015G069301.1.v4.1 564 367.907 0 0 Potri.010G195200.1.v4.1 1773 1576.11 572 7.28936 Potri.012G127500.1.v4.1 977 780.115 7060 181.772 ==> SRR26075318.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 345 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 745 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 115 Potri.001G416900.v4.1 3 Potri.001G452600.v4.1 1333 SRR26075318 completed mapping pipeline successfully