Starting /dee2/code/volunteer_pipeline.sh SRR26075319 current disk space = 3051469594624 free memory = 1440565264 SRR26075319 SRAfilesize 70a70a961383c54515ab350ea8fc3688 SRR26075319.sra SRR26075319.sra file validated SRR26075319 is paired end SRR26075319 is conventional basespace SRR26075319 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075319_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6295 37.0 37.0 37.0 37.0 37.0 2 36.5835 37.0 37.0 37.0 37.0 37.0 3 36.5925 37.0 37.0 37.0 37.0 37.0 4 36.6395 37.0 37.0 37.0 37.0 37.0 5 36.6495 37.0 37.0 37.0 37.0 37.0 6 36.6745 37.0 37.0 37.0 37.0 37.0 7 36.6435 37.0 37.0 37.0 37.0 37.0 8 36.616 37.0 37.0 37.0 37.0 37.0 9 36.6885 37.0 37.0 37.0 37.0 37.0 10-14 36.65815 37.0 37.0 37.0 37.0 37.0 15-19 36.6272 37.0 37.0 37.0 37.0 37.0 20-24 36.588499999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.54880000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.528099999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.4829 37.0 37.0 37.0 37.0 37.0 40-44 36.474700000000006 37.0 37.0 37.0 37.0 37.0 45-49 36.397 37.0 37.0 37.0 37.0 37.0 50-54 36.354 37.0 37.0 37.0 37.0 37.0 55-59 36.3655 37.0 37.0 37.0 37.0 37.0 60-64 36.2878 37.0 37.0 37.0 37.0 37.0 65-69 36.221500000000006 37.0 37.0 37.0 37.0 37.0 70-74 36.2066 37.0 37.0 37.0 37.0 37.0 75-79 36.187200000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.1367 37.0 37.0 37.0 37.0 37.0 85-89 36.090799999999994 37.0 37.0 37.0 37.0 37.0 90-94 36.0188 37.0 37.0 37.0 37.0 37.0 95-99 35.99679999999999 37.0 37.0 37.0 37.0 37.0 100-104 35.974000000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.8401 37.0 37.0 37.0 37.0 37.0 110-114 35.7539 37.0 37.0 37.0 37.0 37.0 115-119 35.6339 37.0 37.0 37.0 37.0 37.0 120-124 35.7448 37.0 37.0 37.0 37.0 37.0 125-129 35.57110000000001 37.0 37.0 37.0 37.0 37.0 130-134 35.531 37.0 37.0 37.0 37.0 37.0 135-139 35.4417 37.0 37.0 37.0 34.6 37.0 140-144 35.291000000000004 37.0 37.0 37.0 29.8 37.0 145-149 35.2827 37.0 37.0 37.0 32.2 37.0 150-151 35.192 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 5.0 24 3.0 25 3.0 26 4.0 27 10.0 28 13.0 29 17.0 30 27.0 31 37.0 32 53.0 33 90.0 34 161.0 35 437.0 36 2951.0 37 188.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.207310966449676 14.897346019028543 7.01051577366049 39.88482724086129 2 17.45 14.875 35.6 32.074999999999996 3 17.525 17.9 27.85 36.725 4 22.175 21.625 24.825 31.374999999999996 5 23.150000000000002 30.675 24.95 21.224999999999998 6 22.2 34.9 22.5 20.4 7 16.475 28.025 39.125 16.375 8 16.5 26.450000000000003 32.074999999999996 24.975 9 20.150000000000002 23.425 34.025 22.400000000000002 10-14 19.420971048552428 29.80149007450372 28.07140357017851 22.706135306765336 15-19 20.205000000000002 27.310000000000002 28.225 24.26 20-24 19.715 28.49 27.435 24.36 25-29 20.18 28.67 26.935 24.215 30-34 20.305 27.200000000000003 27.82 24.675 35-39 20.599999999999998 27.765 27.450000000000003 24.185000000000002 40-44 20.105 29.054999999999996 27.105 23.735 45-49 20.849999999999998 27.439999999999998 27.800000000000004 23.91 50-54 19.98 28.58 27.435 24.005000000000003 55-59 21.07 27.455000000000002 27.425 24.05 60-64 21.154999999999998 27.839999999999996 27.96 23.044999999999998 65-69 20.395 27.279999999999998 28.025 24.3 70-74 20.915 26.945000000000004 28.27 23.87 75-79 20.11 27.27 27.765 24.855 80-84 21.709999999999997 27.3 26.955000000000002 24.035 85-89 21.29 27.485 27.584999999999997 23.64 90-94 20.555 27.525 28.365000000000002 23.555 95-99 20.535 27.855 27.750000000000004 23.86 100-104 21.145 27.6 27.794999999999998 23.46 105-109 21.565 27.465 27.084999999999997 23.885 110-114 21.709999999999997 28.175 26.775 23.34 115-119 21.58 28.055000000000003 27.12 23.244999999999997 120-124 21.015 27.05 27.750000000000004 24.185000000000002 125-129 21.490000000000002 27.3 27.125 24.085 130-134 21.205 28.515 26.22 24.060000000000002 135-139 21.905 28.194999999999997 26.179999999999996 23.72 140-144 22.035 27.675 25.974999999999998 24.315 145-149 20.62 28.975 26.05 24.355 150-151 22.35 27.400000000000002 26.25 24.0 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 0.5 24 0.5 25 0.5 26 0.0 27 1.0 28 1.5 29 5.5 30 9.5 31 9.5 32 29.5 33 39.0 34 37.0 35 47.0 36 76.5 37 117.0 38 125.0 39 131.0 40 173.5 41 202.0 42 238.0 43 287.5 44 301.5 45 281.0 46 259.5 47 268.5 48 252.5 49 195.5 50 179.5 51 162.5 52 120.5 53 96.0 54 77.5 55 79.0 56 55.5 57 30.5 58 24.0 59 13.0 60 10.5 61 12.5 62 10.0 63 5.5 64 5.0 65 7.5 66 5.0 67 2.5 68 3.5 69 2.0 70 1.0 71 0.5 72 0.5 73 2.5 74 2.0 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.175000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 64.29433051869722 39.975 2 20.788098110172896 25.85 3 9.207880981101729 17.175 4 3.297145154804986 8.200000000000001 5 1.5681544028950543 4.875 6 0.2814636107760354 1.05 7 0.2814636107760354 1.225 8 0.12062726176115801 0.6 9 0.08041817450743868 0.44999999999999996 >10 0.08041817450743868 0.6 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CACCTGGAACCATTGTTTTCTTATCCAAGCTTCCAGCATTCCCAAAATAG 14 0.35000000000000003 No Hit CAACTTCTTCATTACCAAAACCCCCACGACGGCCACCACGGAAACCACCA 10 0.25 No Hit GCTCCCTCAGCCTGCGAATTGTGCTCCTCACTGTCACAGCACTGGCAGTG 9 0.22499999999999998 No Hit GTGGCATCACTGGAGGTCCTTGATAATAACCTTGAGCAGGGTATGGATAA 9 0.22499999999999998 No Hit TCTTTCTTTGCCACTTCCTCGAACATAACATCTTCCAAAGTGTAGTAAAA 8 0.2 No Hit CTCTGCTGCGCCATGTCTCTTCAATAAAACAACCGATCACTACTCCTTTC 8 0.2 No Hit GCACCTTCTAGGATCAGCAACAAGCAAAGTCCTATCATACCTCACCAAAA 8 0.2 No Hit GTTTTGTTCAAACACGTGCTACCAGCTCTCAGAGGAGAAGGCAAGTCTCT 7 0.17500000000000002 No Hit CCTAAACAACTTGGCTTCTGCTGATCAAAAGTCCACTTGTCTCCCATCTC 7 0.17500000000000002 No Hit ATCAACTATTTCTTCTCTCAACTTTGCTTTCAATTCTTTTACCTTTTGTG 7 0.17500000000000002 No Hit GCTTCAAAATGCACCCTTGTAGAGTTAAAGGGCAAAGTATAAAAAAAAGT 7 0.17500000000000002 No Hit CTAGTGATTCAAAACCTGATCAGATCAGATCCTCTCCCAGCTAGAAGCAA 7 0.17500000000000002 No Hit GTTGAGAGATCAAGATCTGTTATCACACTCCCATTTCCACCTTCGGGACA 7 0.17500000000000002 No Hit CCAGAATACCACAAACACTAGAGGTTGTTATGATGCTGCCTTGTCGAGCA 7 0.17500000000000002 No Hit ATCTACCTTGTGATCAAGAAAGACAAGAAGATGATCAGGGACGCCCTTGA 6 0.15 No Hit CCATAAATACCCAACACACCAGCCATAACAACTGGAACAATCGACTTCAT 6 0.15 No Hit CCTCCTTTGCCCATGTTTGCTATATCCTCTTTGAACGCCGACACAAACAG 6 0.15 No Hit CTTCATCGCAAATAATCACTACTCAACAGCCCTTTGTCTCACAAGTTATT 6 0.15 No Hit CCAGAACCTTTTGCAGGTGCATTTGACCTAGCCACACTACTCAAACCAGA 6 0.15 No Hit GTACCCACGAGCCAGAGTTTGGTGATCAGCTGTGAAAGGTGATGCTGTTA 6 0.15 No Hit CAGCAATTCATTGGGACTACAATAGGTTGAAACATATATTGCTAGAGCAT 6 0.15 No Hit CTGTAGTAGTTGTTGTCTAGAACCATGGGTGTGCCTCTGTCATTCCTCAC 5 0.125 No Hit GCCCTTTCGTTGACATCAGAGGTAAAAACAAGCCCTAAGCGAAGGTGTGC 5 0.125 No Hit GTCGAAATCAGACATGAAAACTTAACTGTTCAAGGAAGTCCAACAACCTG 5 0.125 No Hit TGGCAAGGAATTTTAAAACTGTCTCAAACAATTCCAAGCATTCCCCAAGA 5 0.125 No Hit AGCAGCTAAGCCAAGAGGATCAAAGAAATTGCCTCCTGGGTAGAGCCTTT 5 0.125 No Hit CCCTTCAGTTCTTTCTTATTGTAGTGACGGACCATCACTCCCATACTAGC 5 0.125 No Hit CTGGGTTGTGAAAAATTCTTTGCTTCATCCAATTTACAATTTACCTCAGT 5 0.125 No Hit ACCAGTTTCTGTTGTTTCTTCGCAAAAGTCAGTTGCCACCTTTGCGCAGT 5 0.125 No Hit GCCGTCCATAAATCTGAAAGATTGGTTGGAGTGCTCGAAGCTCACCACTA 5 0.125 No Hit CCTAAGTTCAGCTGGATCAATCTCTGCTACATAATTAGCAGGCCTGTAAT 5 0.125 No Hit CAGCAACATTCTTAACATTGAAACCAACATTGTCACCTGGAAGGGCCTCC 5 0.125 No Hit CTTTAAATCTTCATCTAAATCTAAATCAGCAACTGCCTTGGCAACTGCAT 5 0.125 No Hit AATGAGGGGATCTGGGTAGCGGATGGTGTCATTGGCCTTAATGAGGGGAT 5 0.125 No Hit CAAGAACAGATAATTTAAACGAGTTCAACAAATTATTAAAAGCAACCATT 5 0.125 No Hit CTCCTGCTTTTTTCTTCAGTTCATACCACCTCCGTGCATTGGCATGTGCA 5 0.125 No Hit AGTTAAACCAACATTAATACCACAACTATCTTAATTGCCACTGACTAGCA 5 0.125 No Hit CCCATGCCTGTCTGCAAGATTGTAGCCATGCTTGTAATAGTTGTAACTCT 5 0.125 No Hit ATCTCACAGACCATCAACACCAGGGCATGAGATCCGAAAAGCGTTGTTAA 5 0.125 No Hit CTCGTCAAGCTTTTCTGGTGTAAATGGGTAGGCTTCGATACCATGTTCTT 5 0.125 No Hit CTTCCTTTGCCACAGTCCATGTAATAACCTTCCCATCCTGCGAGGCACTA 5 0.125 No Hit GCCCAGACAGTAGAAGAGTGACCATTGTTAGATTCACCTAAGGATTGAAC 5 0.125 No Hit AAAAGACTCACAAGACTCACGATCGAGGACATTCATCATCTCATCACTCA 5 0.125 No Hit GTCAGAGACTAGTGATGATGGAGCTGTGAAGAGAGCGTAATCAAGACGAA 5 0.125 No Hit GTGGGGGCGAGCCAATACCAATTTCTCCCAAGTATTGAGCATCCAAATAG 5 0.125 No Hit CTCGAGAGTGATCGTCTTGCCGGTCAAGGTCTTCACGAAAATCTGCATGC 5 0.125 No Hit ATGCCTTTTCCTTGTCAGTGAAAACTCCAGTTGACTCCACAATATACTCA 5 0.125 No Hit ATATGACAAACATAAACCTAAAACCAACATACCGAATGACCAAAGGCAAA 5 0.125 No Hit CTCGTAATTATTGTTAGGGATGAAAGGGATGAATCTGCGGTTAAATAAGT 5 0.125 No Hit CATTTTCTGTTTACAGTCTTCCCTGGCCTAGCAACACCACTTAAAAGCAG 5 0.125 No Hit GAGGGAGTAAGTTGAGAAACATACCAGTTGTGAAAGTAATTATCAAGATA 5 0.125 No Hit CTCTGTTCTTAGCTTCTTACTCTGCACGCTTTTCTTCAACCATCTCCAAG 5 0.125 No Hit CTGCTGGCTGACGGTGTGGACGGCGCTGTTCGGCTGAAGAAGGAGACCGT 5 0.125 No Hit CTCAAAGGAAAGAATTGGTGGTCTGTTGAGATGTCGGGTTCTTCGCAGGA 5 0.125 No Hit TAGGAAGGAAAGCCGTGCATTTTGCATTTAGAGAACAAACATTTCAAGCT 5 0.125 No Hit GAAGGGTACTCTTTTAAAATTGCTGCCTGAAGAGTAGTAAATATTGAAAC 5 0.125 No Hit GTATGATTCAACTTCGTCTGGAAAAGTCTGTTCAAGTTCCTCCAGTAGAT 5 0.125 No Hit GCATTTGAAGGGCAATCAGCAGCAAAACCAAGGTTCCGGAGTCTGTTGTC 5 0.125 No Hit AGTTAAGAATGGCATGGACTTTCTTGGTGCTGTATCCACATATATCTGGT 5 0.125 No Hit GCTTTGACTTTCCCACAGTCCTTGCATCCCCAAATTCCAACAGCCTTCCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0375 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.16249999999999998 0.0 0.0 0.0 0.0 86-87 0.1875 0.0 0.0 0.0 0.0 88-89 0.25 0.0 0.0 0.0 0.0 90-91 0.275 0.0 0.0 0.0 0.0 92-93 0.4 0.0 0.0 0.0 0.0 94-95 0.6125 0.0 0.0 0.0 0.0 96-97 1.0625 0.0 0.0 0.0 0.0 98-99 1.1875 0.0 0.0 0.0 0.0 100-101 1.325 0.0 0.0 0.0 0.0 102-103 1.8125 0.0 0.0 0.0 0.0 104-105 2.2125 0.0 0.0 0.0 0.0 106-107 2.3625 0.0 0.0 0.0 0.0 108-109 2.675 0.0 0.0 0.0 0.0 110-111 2.8625 0.0 0.0 0.0 0.0 112-113 3.0625 0.0 0.0 0.0 0.0 114-115 3.375 0.0 0.0 0.0 0.0 116-117 3.6375 0.0 0.0 0.0 0.0 118-119 4.15 0.0 0.0 0.0 0.0 120-121 4.5875 0.0 0.0 0.0 0.0 122-123 5.0 0.0 0.0 0.0 0.0 124-125 5.800000000000001 0.0 0.0 0.0 0.0 126-127 6.6625 0.0 0.0 0.0 0.0 128-129 7.1875 0.0 0.0 0.0 0.0 130-131 7.9 0.0 0.0 0.0 0.0 132-133 8.4 0.0 0.0 0.0 0.0 134-135 9.0375 0.0 0.0 0.0 0.0 136-137 9.4875 0.0 0.0 0.0 0.0 138-139 10.0125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR26075319 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075319_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.45525 37.0 37.0 37.0 37.0 37.0 2 36.3635 37.0 37.0 37.0 37.0 37.0 3 36.402 37.0 37.0 37.0 37.0 37.0 4 36.4865 37.0 37.0 37.0 37.0 37.0 5 36.343 37.0 37.0 37.0 37.0 37.0 6 36.4305 37.0 37.0 37.0 37.0 37.0 7 36.451 37.0 37.0 37.0 37.0 37.0 8 36.415 37.0 37.0 37.0 37.0 37.0 9 36.4745 37.0 37.0 37.0 37.0 37.0 10-14 36.4597 37.0 37.0 37.0 37.0 37.0 15-19 36.4305 37.0 37.0 37.0 37.0 37.0 20-24 36.4019 37.0 37.0 37.0 37.0 37.0 25-29 36.341899999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.269600000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.2382 37.0 37.0 37.0 37.0 37.0 40-44 36.1947 37.0 37.0 37.0 37.0 37.0 45-49 36.1527 37.0 37.0 37.0 37.0 37.0 50-54 36.0649 37.0 37.0 37.0 37.0 37.0 55-59 36.0753 37.0 37.0 37.0 37.0 37.0 60-64 36.1114 37.0 37.0 37.0 37.0 37.0 65-69 36.040499999999994 37.0 37.0 37.0 37.0 37.0 70-74 35.9577 37.0 37.0 37.0 37.0 37.0 75-79 35.8455 37.0 37.0 37.0 37.0 37.0 80-84 35.9877 37.0 37.0 37.0 37.0 37.0 85-89 35.9263 37.0 37.0 37.0 37.0 37.0 90-94 35.78395 37.0 37.0 37.0 37.0 37.0 95-99 35.924899999999994 37.0 37.0 37.0 37.0 37.0 100-104 35.806 37.0 37.0 37.0 37.0 37.0 105-109 35.746300000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.6943 37.0 37.0 37.0 37.0 37.0 115-119 35.6089 37.0 37.0 37.0 37.0 37.0 120-124 35.5303 37.0 37.0 37.0 37.0 37.0 125-129 35.57505 37.0 37.0 37.0 37.0 37.0 130-134 35.46509999999999 37.0 37.0 37.0 37.0 37.0 135-139 35.39985 37.0 37.0 37.0 34.6 37.0 140-144 35.3344 37.0 37.0 37.0 37.0 37.0 145-149 35.242599999999996 37.0 37.0 37.0 34.6 37.0 150-151 34.945375 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 2.0 15 3.0 16 4.0 17 3.0 18 3.0 19 1.0 20 2.0 21 2.0 22 9.0 23 6.0 24 2.0 25 7.0 26 7.0 27 17.0 28 10.0 29 10.0 30 25.0 31 27.0 32 43.0 33 75.0 34 160.0 35 584.0 36 2732.0 37 264.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.30673004753565 20.06504878658994 11.908931698774081 25.719289467100324 2 28.749999999999996 25.825 27.800000000000004 17.625 3 20.825 27.200000000000003 34.0 17.974999999999998 4 24.9 33.550000000000004 23.175 18.375 5 28.125 36.55 19.675 15.65 6 22.475 39.45 20.549999999999997 17.525 7 21.925 21.975 35.625 20.474999999999998 8 21.349999999999998 27.35 27.325 23.974999999999998 9 22.425 24.825 29.025000000000002 23.724999999999998 10-14 23.345 30.020000000000003 25.27 21.365000000000002 15-19 23.87 27.810000000000002 27.79 20.53 20-24 23.635 27.515 26.950000000000003 21.9 25-29 23.97 27.435 26.905 21.69 30-34 23.494999999999997 27.26 27.944999999999997 21.3 35-39 24.48 28.110000000000003 26.235000000000003 21.175 40-44 23.7 27.750000000000004 27.455000000000002 21.095 45-49 24.705 27.150000000000002 27.095000000000002 21.05 50-54 23.64 28.765 27.1 20.495 55-59 24.075 28.13 26.91 20.885 60-64 23.830000000000002 27.105 27.43 21.634999999999998 65-69 24.33 28.749999999999996 26.215 20.705000000000002 70-74 23.630000000000003 28.225 27.445000000000004 20.7 75-79 24.044999999999998 27.55 27.365000000000002 21.04 80-84 24.740000000000002 28.33 26.945000000000004 19.985 85-89 24.21 27.534999999999997 27.295 20.96 90-94 23.736186809340467 28.196409820491024 26.721336066803342 21.346067303365167 95-99 24.48 27.905 26.695 20.919999999999998 100-104 25.124999999999996 27.87 25.97 21.035 105-109 24.605 27.11 27.41 20.875 110-114 24.805 28.294999999999998 26.985 19.915 115-119 24.8 27.860000000000003 27.295 20.044999999999998 120-124 25.340068013602718 28.09561912382477 27.38047609521904 19.183836767353473 125-129 25.216260813040652 28.291414570728534 26.041302065103256 20.451022551127558 130-134 25.685000000000002 28.765 26.085 19.465 135-139 25.924073425698996 27.23453208623018 27.419596858900615 19.42179762917021 140-144 25.330198118871323 27.756653992395435 25.960576345807485 20.952571542925753 145-149 27.014312881593433 27.31958762886598 25.95836252627365 19.70773696326694 150-151 27.141963727329582 27.567229518449032 27.029393370856784 18.2614133833646 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 0.5 21 1.5 22 1.5 23 1.0 24 0.5 25 0.5 26 1.5 27 2.5 28 3.5 29 5.0 30 9.5 31 15.0 32 16.0 33 20.0 34 36.0 35 51.0 36 58.5 37 79.0 38 110.0 39 140.5 40 189.5 41 242.5 42 271.5 43 265.0 44 275.0 45 304.0 46 300.5 47 293.0 48 237.0 49 191.5 50 172.0 51 134.0 52 114.5 53 83.5 54 61.0 55 70.5 56 65.5 57 39.0 58 29.0 59 20.0 60 13.5 61 9.0 62 5.5 63 8.0 64 5.0 65 3.5 66 2.5 67 1.0 68 1.5 69 1.5 70 2.0 71 2.5 72 1.5 73 0.0 74 2.0 75 3.0 76 1.5 77 0.5 78 0.5 79 0.5 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.5 86 1.0 87 1.5 88 1.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.5 98 1.0 99 0.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.005 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.02 125-129 0.005 130-134 0.0 135-139 0.034999999999999996 140-144 0.06 145-149 0.09 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.625 #Duplication Level Percentage of deduplicated Percentage of total 1 65.58882235528942 41.075 2 19.800399201596807 24.8 3 8.742514970059881 16.425 4 3.473053892215569 8.7 5 1.437125748502994 4.5 6 0.39920159680638717 1.5 7 0.19960079840319359 0.8750000000000001 8 0.11976047904191617 0.6 9 0.1596806387225549 0.8999999999999999 >10 0.07984031936127745 0.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGAAGACTCTTCTGTTGACATTGCGGGCAGAAGGTTCCATAGCGGGACA 13 0.325 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 12 0.3 No Hit ATTTCCAGCCGCTGCTTTCCTTCAGTCCACAAGCAGAGACCCGTCAAGGC 9 0.22499999999999998 No Hit GCTAGATACTAGTAGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGTTC 9 0.22499999999999998 No Hit TGAGAGTTCATTCGGTAACTCTGGAGCACCAACTGTTCAAGGTGCTGCTG 9 0.22499999999999998 No Hit CCAAAAGCACTACTGCACCTCCTTCAATCTTCACATCTGAAATTCTTGAG 9 0.22499999999999998 No Hit AACTAGTTGAGCCTGAAATCCTCCGATTCAAGGCCTATGAACCAATCCTT 8 0.2 No Hit GTGTGGATTAGGTCTGTTTGGTTGCTAGCAAAATTCGGTGCAGAATCTCC 8 0.2 No Hit CAAACCGGAACCAAGAACTACATTGGCCCTTTCGAGTCATTTTATAATTT 8 0.2 No Hit CTAACCTTGCATCAAGTTAAGGCCTTGGAGAAGAATTTTGAGGTCGATAA 7 0.17500000000000002 No Hit CACACATTTGGCAGGGCAAAATGTTCTACATTCGACTTCCGATTGTATGA 7 0.17500000000000002 No Hit GGAAAACACTGGTGCACACGGCAAGAATATCTCCTCAGCAAAGGAGGGGA 7 0.17500000000000002 No Hit GCCATGCTGTGCTGGCGCTGCAAGAGGCAGCTGAGGCATACCTTGTGGGT 7 0.17500000000000002 No Hit GATTGACCCTTCGTGATCTCTCTCTTACACTCACACAAACTCTCATGTTA 7 0.17500000000000002 No Hit GACACGATGGCTAAGTTTGCTGTGGCTAATCTCTTGATCCTTCTTTTGAA 6 0.15 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 6 0.15 No Hit GGGCTCCCAATGGGATCAAAACTGGTTGGATCATGCACGAGTTTCGTCTC 6 0.15 No Hit TATGGCCATGCATGGACTGGTTGCAGCATAGAGACAATCGAAGTCTTTCC 6 0.15 No Hit TACGATTCAGATTTTGGTTTCCGTTTCCAATCTTTATGATTATGCGAAAC 6 0.15 No Hit CAAACATAGAACTTGATACGCCTCCAGAATTCAGACTAAGCCAGCTTGAA 6 0.15 No Hit CCTTATCTCTCCTAGTCATCATATCTTTTCTTTTCTTTTTTTTCCTTTCC 6 0.15 No Hit CAACAACAGCAACCTTCAAATCATTTTGGAAACACTTATGGAAGCAGTGT 6 0.15 No Hit CTGCAAACTTCAGATCTAAAACGACACTGTCTCAGATCCAGTTTATCAAT 6 0.15 No Hit GGATATTGTACTCCAGTGATGCTCTTTGTATTCGTCGATGATTTCTTGGA 6 0.15 No Hit GCTATCCCTCTCAATAAGGGTGGACATAGTGTTCCTGTGCATGATGGCAA 5 0.125 No Hit TTCAGAAAGAGAGCACGCTGCACCTCGTCCTGCGTCTCCGTGGTGGCATG 5 0.125 No Hit GGAAAAGGTAACTGTAGTGGGATATGTTGATCGAAACAAGGTACTCAAGG 5 0.125 No Hit GTTACGACCGCCGAGGTTTGTGACGCAAATCCGCAGCTAATTGTTAGTGG 5 0.125 No Hit GTGAGCCCAAGAGACCCACAGACAAGCCTCTCCGTCTTCCACTTCAGGAT 5 0.125 No Hit CAGAAATACTAGCTTCTTTAAGGTCTCTAATGGCGTCTCATTCTCCACCG 5 0.125 No Hit GGTTATGGAGCATTTCCCTTAAGCATCATTGAGTATCTTTGGAGAGCTAA 5 0.125 No Hit GTGTCCTGGATCATAAAAACTGGGAGTTCTTTGCTGAGGGCAATGCTAAA 5 0.125 No Hit GGCGCACCCTGGCAGACTACAATATCCAGAAGGAGTCCACACTTCACCTT 5 0.125 No Hit GTTTGAAGAAGCGCCGTCTAGACCTTGATACTATTAAGGCTGCCAGAATT 5 0.125 No Hit GGTTACGCGGCTGCTGCACCGATCAGTGCTGCTGTAACAGCGAGCTTCGG 5 0.125 No Hit GTTTCAGTTTTGCAAGGACATACACAAGATGTTAAAATGGTTAAATGGCA 5 0.125 No Hit AAGAGCTGCGAGAAGCTAGTGCGGTATTTTGAACTTAGAACCATTCCTAA 5 0.125 No Hit GCCATTTCAAGGCATGGCAAGCTAGACGTTATGTTCAATAATGCTGGCAT 5 0.125 No Hit GTCTGCGTAAGCAGATAAAAAAGATGGAGGTTAGTCAGCACAGCAAGTTT 5 0.125 No Hit GGTGGGATGTACCCTTTCAGAGACATCTAACCCTAGTTCTGTAGCTAATT 5 0.125 No Hit TTTACATCTTCTCCGGCATCTGTAGTAGTTCTCACCTCCTCGGGTGATCG 5 0.125 No Hit CGAGTGGCTCACTGGAGTTACCTGGCAAGATGCTGGAAAGGTGGAATTAG 5 0.125 No Hit GCTAGCTGGGTAATTTTTTTGTAGGTGCACTATCACAAGGGGTGTTATAA 5 0.125 No Hit AAGGATGAGAAGAAGGCAGGAAACCCTGTCGCTGGCTTAATTGACAAGCT 5 0.125 No Hit CCTCAAAAGCATTTTCTCTGGTAAAACGTCCAAAACCCAAACTCAACCTG 5 0.125 No Hit AAAAAGTCAGCCAATCCTCTCTTCTGGGCTGCCCCCTTCTCCTCGATGGC 5 0.125 No Hit GGAAATTGTGTTTTTGCCAATTTAAGACCTAATTTAATAGTTAAACCATT 5 0.125 No Hit CCCAAAGACAAATGAGAGCTTTCGTCTCCTCTATGACACCAAAGGCCGCT 5 0.125 No Hit CTTTGATTTGCTACATGTCAAACTCAAGTACTTCTCTTCCTTGTTTACAA 5 0.125 No Hit GGAATGGAGACCCCAATGACAAAGGTATCCAAACAAGTGAAGACTATAGG 5 0.125 No Hit CTTGTTGGTTCTGGTTTGCTTGACCCTGCTCAGAAGCTTTGCTCAGGTGG 5 0.125 No Hit GGAGATGCGAATCCAGACCCTGCAAAAGCAGAGGAATTGTATGCAAGCAA 5 0.125 No Hit TGTCACTTTACTTAACACTTGAGCTGCATAGAATGTCTACAGTCAATTTG 5 0.125 No Hit TAAGAAGTCGGATCAAAATCATGGGCACACTGGTATCAGTTTCAGGAGCT 5 0.125 No Hit AGCGACTAAAAATCTTGCACTGCTTCCATGCATTAGGTGTGGAGGAGGTC 5 0.125 No Hit TATCCGAAGTGTTCTGTGGAACAATTTCTTGAAGAATCAAGAAACATGGC 5 0.125 No Hit CATACTGTTTCTCTCTGTGAATCATGGGAAAGATCAAGATTGGAATCAAC 5 0.125 No Hit AAATGGAGGAATTGAAGACTTTGGAAAATACATATTGTAATTACTTCAAC 5 0.125 No Hit ATTGAATCGGAAAACTAACTTTTCATTATAAAGAGTTTATTTAGTTATAA 5 0.125 No Hit AATGAAAAGCTCTTCGTGGTGTTCCAAGGTGTTTCAAATGTAGCTGGTGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0375 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.16249999999999998 0.0 0.0 0.0 0.0 86-87 0.1875 0.0 0.0 0.0 0.0 88-89 0.25 0.0 0.0 0.0 0.0 90-91 0.275 0.0 0.0 0.0 0.0 92-93 0.4 0.0 0.0 0.0 0.0 94-95 0.6125 0.0 0.0 0.0 0.0 96-97 1.0625 0.0 0.0 0.0 0.0 98-99 1.1875 0.0 0.0 0.0 0.0 100-101 1.325 0.0 0.0 0.0 0.0 102-103 1.8125 0.0 0.0 0.0 0.0 104-105 2.2375 0.0 0.0 0.0 0.0 106-107 2.3875 0.0 0.0 0.0 0.0 108-109 2.675 0.0 0.0 0.0 0.0 110-111 2.8375 0.0 0.0 0.0 0.0 112-113 3.0250000000000004 0.0 0.0 0.0 0.0 114-115 3.3 0.0 0.0 0.0 0.0 116-117 3.575 0.0 0.0 0.0 0.0 118-119 4.1125 0.0 0.0 0.0 0.0 120-121 4.5625 0.0 0.0 0.0 0.0 122-123 5.0 0.0 0.0 0.0 0.0 124-125 5.824999999999999 0.0 0.0 0.0 0.0 126-127 6.7125 0.0 0.0 0.0 0.0 128-129 7.25 0.0 0.0 0.0 0.0 130-131 7.975 0.0 0.0 0.0 0.0 132-133 8.425 0.0 0.0 0.0 0.0 134-135 9.0625 0.0 0.0 0.0 0.0 136-137 9.5125 0.0 0.0 0.0 0.0 138-139 10.0375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATGAG 10 0.006830828 145.0 5 AAGAAAT 10 0.006830828 145.0 5 CCTTAAC 10 0.006830828 145.0 1 >>END_MODULE Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017401 spots for SRR26075319.sra Written 1017401 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra Read 1017391 spots for SRR26075319.sra Written 1017391 spots for SRR26075319.sra SRR ids: ['SRR26075319.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_4wd38kn9 SRR26075319.sra spots: 20347830 blocks: [[1, 1017391], [1017392, 2034782], [2034783, 3052173], [3052174, 4069564], [4069565, 5086955], [5086956, 6104346], [6104347, 7121737], [7121738, 8139128], [8139129, 9156519], [9156520, 10173910], [10173911, 11191301], [11191302, 12208692], [12208693, 13226083], [13226084, 14243474], [14243475, 15260865], [15260866, 16278256], [16278257, 17295647], [17295648, 18313038], [18313039, 19330429], [19330430, 20347830]] SRR26075319 file size 7509605 SRR26075319 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075319 SRR26075319_1.fastq SRR26075319_2.fastq Input file: SRR26075319_1.fastq Paired file: SRR26075319_2.fastq trimmed: SRR26075319-trimmed-pair1.fastq, SRR26075319-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 17:44:12 2025 >> started Tue Feb 11 17:44:36 2025 >> done (23.257s) 20347830 read pairs processed; of these: 60 ( 0.00%) short read pairs filtered out after trimming by size control 23828 ( 0.12%) empty read pairs filtered out after trimming by size control 20323942 (99.88%) read pairs available; of these: 2946692 (14.50%) trimmed read pairs available after processing 17377250 (85.50%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 7 0.00% 20 4 0.00% 21 6 0.00% 22 5 0.00% 23 10 0.00% 24 11 0.00% 25 10 0.00% 26 13 0.00% 27 12 0.00% 28 8 0.00% 29 14 0.00% 30 22 0.00% 31 12 0.00% 32 18 0.00% 33 21 0.00% 34 19 0.00% 35 19 0.00% 36 22 0.00% 37 29 0.00% 38 37 0.00% 39 32 0.00% 40 40 0.00% 41 57 0.00% 42 46 0.00% 43 46 0.00% 44 54 0.00% 45 53 0.00% 46 73 0.00% 47 48 0.00% 48 92 0.00% 49 83 0.00% 50 98 0.00% 51 106 0.00% 52 137 0.00% 53 164 0.00% 54 129 0.00% 55 188 0.00% 56 148 0.00% 57 189 0.00% 58 223 0.00% 59 275 0.00% 60 293 0.00% 61 351 0.00% 62 421 0.00% 63 480 0.00% 64 497 0.00% 65 521 0.00% 66 664 0.00% 67 731 0.00% 68 844 0.00% 69 960 0.00% 70 1053 0.01% 71 1275 0.01% 72 1382 0.01% 73 1639 0.01% 74 1914 0.01% 75 2074 0.01% 76 2413 0.01% 77 2667 0.01% 78 2758 0.01% 79 3256 0.02% 80 3889 0.02% 81 4066 0.02% 82 4692 0.02% 83 5371 0.03% 84 5974 0.03% 85 6747 0.03% 86 7345 0.04% 87 8021 0.04% 88 8184 0.04% 89 9641 0.05% 90 10001 0.05% 91 10545 0.05% 92 11762 0.06% 93 12864 0.06% 94 14060 0.07% 95 15411 0.08% 96 16199 0.08% 97 18072 0.09% 98 18272 0.09% 99 19563 0.10% 100 20622 0.10% 101 21942 0.11% 102 22505 0.11% 103 23998 0.12% 104 25826 0.13% 105 27312 0.13% 106 28773 0.14% 107 30316 0.15% 108 31652 0.16% 109 32292 0.16% 110 33483 0.16% 111 34626 0.17% 112 35574 0.18% 113 36559 0.18% 114 38084 0.19% 115 41040 0.20% 116 41626 0.20% 117 43085 0.21% 118 44827 0.22% 119 45568 0.22% 120 46665 0.23% 121 47296 0.23% 122 48138 0.24% 123 49844 0.25% 124 51497 0.25% 125 52892 0.26% 126 55928 0.28% 127 57576 0.28% 128 58428 0.29% 129 59328 0.29% 130 60966 0.30% 131 60921 0.30% 132 61436 0.30% 133 63318 0.31% 134 63840 0.31% 135 66159 0.33% 136 67948 0.33% 137 68672 0.34% 138 70346 0.35% 139 71878 0.35% 140 73516 0.36% 141 74146 0.36% 142 76336 0.38% 143 75509 0.37% 144 77218 0.38% 145 78239 0.38% 146 79426 0.39% 147 80219 0.39% 148 82885 0.41% 149 82340 0.41% 150 84618 0.42% 151 17377250 85.50% 20323942 reads passed initial QC criterion=sequence-density sequence-density=0.43 sequence-density-rank=1 fanout-score=2.24 fanout-score-rank=26 prefix-density=0.44 prefix-fanout=2.2 sequence=GGAATTGAATTAATGAACTTGGTGGCAGCAAGGAAGGCGTTGTAGGAGGCTAAATAGCTAACTCC criterion=fanout-score sequence-density=0.02 sequence-density-rank=29 fanout-score=46.44 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=7.7 sequence=CACCTCCACCACTTCCGCGGGATTGAGCTTCGTTCACGGTGATGTTACGGCCATCGAGGTCCTGACCGTTCATTCCATCAATAGCATCTCTCATTGCCTTCTC criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=2.72 fanout-score-rank=18 prefix-density=0.42 prefix-fanout=2.7 sequence=ATGTACCCTGACTTAGGTTTCTCAGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=27 fanout-score=15.89 fanout-score-rank=1 prefix-density=0.06 prefix-fanout=1.8 sequence=CACAAAGCAGTTGCATTTATCTAAAGTATT SRR26075319 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 17:45:24 Started mapping on | Feb 11 17:45:25 Finished on | Feb 11 17:49:20 Mapping speed, Million of reads per hour | 311.35 Number of input reads | 20323942 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 18189397 Uniquely mapped reads % | 89.50% Average mapped length | 293.67 Number of splices: Total | 17895261 Number of splices: Annotated (sjdb) | 17514378 Number of splices: GT/AG | 17576631 Number of splices: GC/AG | 246097 Number of splices: AT/AC | 16043 Number of splices: Non-canonical | 56490 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.80 Insertion rate per base | 0.02% Insertion average length | 2.63 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 505747 % of reads mapped to multiple loci | 2.49% Number of reads mapped to too many loci | 46477 % of reads mapped to too many loci | 0.23% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 7.52% % of reads unmapped: other | 0.26% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1628798 1628798 1628798 N_multimapping 505747 505747 505747 N_noFeature 490537 17954534 636673 N_ambiguous 189133 1333 99402 UnstrandedReadsAssigned:17509727 PositiveStrandReadsAssigned:233530 NegativeStrandReadsAssigned:17453322 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075319 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075319-trimmed-pair1.fastq SRR26075319-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,323,942 reads, 17,545,949 reads pseudoaligned [quant] estimated average fragment length: 219.055 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,097 rounds 52401 SRR26075319.ke.tsv 34699 SRR26075319.se.tsv 87100 total ==> SRR26075319.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1799.94 1442 42.7158 Potri.005G024800.1.v4.1 1035 816.945 1159 75.6438 Potri.004G059700.1.v4.1 961 742.945 1 0.0717672 Potri.007G009000.2.v4.1 1416 1197.94 0 0 Potri.003G141000.2.v4.1 2943 2724.94 926 18.1191 Potri.016G087400.1.v4.1 270 88.2825 1693.86 1023.02 Potri.015G069301.1.v4.1 564 348.78 0 0 Potri.010G195200.1.v4.1 1773 1554.94 692.785 23.7556 Potri.012G127500.1.v4.1 977 758.945 7802 548.123 ==> SRR26075319.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 63 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 177 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 2 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1395 SRR26075319 completed mapping pipeline successfully