Starting /dee2/code/volunteer_pipeline.sh SRR26075320 current disk space = 3050378346496 free memory = 1580988064 SRR26075320 SRAfilesize b32f86142951849dad13eb80ddd20b0a SRR26075320.sra SRR26075320.sra file validated SRR26075320 is paired end SRR26075320 is conventional basespace SRR26075320 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075320_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5955 37.0 37.0 37.0 37.0 37.0 2 36.584 37.0 37.0 37.0 37.0 37.0 3 36.5455 37.0 37.0 37.0 37.0 37.0 4 36.6805 37.0 37.0 37.0 37.0 37.0 5 36.6425 37.0 37.0 37.0 37.0 37.0 6 36.6325 37.0 37.0 37.0 37.0 37.0 7 36.583 37.0 37.0 37.0 37.0 37.0 8 36.6585 37.0 37.0 37.0 37.0 37.0 9 36.61 37.0 37.0 37.0 37.0 37.0 10-14 36.6344 37.0 37.0 37.0 37.0 37.0 15-19 36.602500000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.4793 37.0 37.0 37.0 37.0 37.0 25-29 36.4476 37.0 37.0 37.0 37.0 37.0 30-34 36.4161 37.0 37.0 37.0 37.0 37.0 35-39 36.331100000000006 37.0 37.0 37.0 37.0 37.0 40-44 36.3238 37.0 37.0 37.0 37.0 37.0 45-49 36.2875 37.0 37.0 37.0 37.0 37.0 50-54 36.2516 37.0 37.0 37.0 37.0 37.0 55-59 36.1697 37.0 37.0 37.0 37.0 37.0 60-64 36.0943 37.0 37.0 37.0 37.0 37.0 65-69 36.0307 37.0 37.0 37.0 37.0 37.0 70-74 36.0393 37.0 37.0 37.0 37.0 37.0 75-79 36.0602 37.0 37.0 37.0 37.0 37.0 80-84 36.0387 37.0 37.0 37.0 37.0 37.0 85-89 35.9128 37.0 37.0 37.0 37.0 37.0 90-94 35.799099999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.8513 37.0 37.0 37.0 37.0 37.0 100-104 35.8001 37.0 37.0 37.0 37.0 37.0 105-109 35.7331 37.0 37.0 37.0 37.0 37.0 110-114 35.578700000000005 37.0 37.0 37.0 37.0 37.0 115-119 35.465199999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.5438 37.0 37.0 37.0 37.0 37.0 125-129 35.411500000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.29449999999999 37.0 37.0 37.0 32.2 37.0 135-139 35.108799999999995 37.0 37.0 37.0 29.8 37.0 140-144 34.994699999999995 37.0 37.0 37.0 25.0 37.0 145-149 34.9962 37.0 37.0 37.0 25.0 37.0 150-151 34.598 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 2.0 18 1.0 19 1.0 20 2.0 21 4.0 22 2.0 23 4.0 24 4.0 25 5.0 26 11.0 27 16.0 28 15.0 29 28.0 30 39.0 31 44.0 32 61.0 33 104.0 34 162.0 35 448.0 36 2848.0 37 199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.216324486730095 14.446670005007512 7.961942914371558 33.37506259389084 2 19.55 13.525 34.55 32.375 3 18.3 18.099999999999998 27.975 35.625 4 20.599999999999998 24.8 25.874999999999996 28.725 5 22.95 28.775000000000002 27.525 20.75 6 23.599999999999998 32.85 23.425 20.125 7 16.275000000000002 29.775000000000002 37.95 16.0 8 17.925 27.675 31.5 22.900000000000002 9 17.974999999999998 24.474999999999998 32.625 24.925 10-14 20.549999999999997 29.635 27.71 22.105 15-19 20.19 28.720000000000002 27.42 23.669999999999998 20-24 20.66 27.805000000000003 27.625 23.91 25-29 19.985 28.29 28.349999999999998 23.375 30-34 19.68 29.255 26.66 24.404999999999998 35-39 20.915 28.24 27.495000000000005 23.35 40-44 20.875 28.315 27.05 23.76 45-49 20.505000000000003 27.88 27.250000000000004 24.365000000000002 50-54 20.135 27.41 28.384999999999998 24.07 55-59 20.445 27.83 27.735 23.990000000000002 60-64 20.31 27.68 27.79 24.22 65-69 20.13 27.334999999999997 28.544999999999998 23.990000000000002 70-74 20.585 27.139999999999997 27.235 25.040000000000003 75-79 20.965 27.54 27.485 24.01 80-84 21.55 27.76 27.37 23.32 85-89 21.39 26.419999999999998 27.994999999999997 24.195 90-94 20.82 27.68 27.985 23.515 95-99 21.82 27.384999999999998 27.095000000000002 23.7 100-104 20.87 28.165000000000003 26.945000000000004 24.02 105-109 20.655 26.695 28.1 24.55 110-114 21.145 27.74 27.534999999999997 23.580000000000002 115-119 21.125 28.835 25.779999999999998 24.26 120-124 22.195 27.82 27.265 22.720000000000002 125-129 21.745 28.095 26.25 23.91 130-134 22.345000000000002 28.28 26.284999999999997 23.09 135-139 22.065 27.345000000000002 26.305 24.285 140-144 21.415 27.27 26.76 24.555 145-149 22.015 27.465 26.82 23.7 150-151 22.3875 27.800000000000004 26.35 23.4625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 1.0 24 2.5 25 2.5 26 2.0 27 4.5 28 6.5 29 9.0 30 16.0 31 21.0 32 20.0 33 40.5 34 49.0 35 56.0 36 72.5 37 82.0 38 135.5 39 156.5 40 171.5 41 211.5 42 239.0 43 258.0 44 261.0 45 263.5 46 278.5 47 274.0 48 223.0 49 204.5 50 182.5 51 145.5 52 131.5 53 98.5 54 63.0 55 56.0 56 57.0 57 54.0 58 40.5 59 20.5 60 22.5 61 15.5 62 3.0 63 4.0 64 4.0 65 5.0 66 7.0 67 5.5 68 2.0 69 8.0 70 7.0 71 0.0 72 1.0 73 1.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.875 #Duplication Level Percentage of deduplicated Percentage of total 1 61.401273885350314 36.15 2 21.48619957537155 25.3 3 9.51167728237792 16.8 4 3.7367303609341826 8.799999999999999 5 2.208067940552017 6.5 6 0.9341825902335457 3.3000000000000003 7 0.5095541401273885 2.1 8 0.16985138004246286 0.8 9 0.0 0.0 >10 0.042462845010615716 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAGTGAGCCAGTTGCCAAAATCACGTTGCCATGCTGAGTCCCATATGGAT 10 0.25 No Hit GCCATGACATGGTTGCTGCATTTTTCTTTTGCGCGAGTTGCATTATTTCA 8 0.2 No Hit TGCTTTAGGATGTTAACGATGAAATTCCTTAGACGATAGAACAGCAATTG 8 0.2 No Hit GCTCAGGATGTTCCAGGCACGGTAGAACCGTGGAACCATAACAACAAGAG 8 0.2 No Hit GTGATGGGAAGCCAGAAAACTTCCTTGGGCGCTTCACTTGGAGAGAACAT 8 0.2 No Hit GAACGTAAAAATCAGAAGTAGAACTCTTCGATCCAAAGACAACTTTGGGT 7 0.17500000000000002 No Hit GGATATTGTGTAAGAGTATATGTTACAGATAAGCGAAGGCTGATGGAAAG 7 0.17500000000000002 No Hit GTGGTGCTTTCTGAGATACCTAAGTCAGGGTACATGCCACATTTGCAGCC 7 0.17500000000000002 No Hit ATCTTGAAGGTGGACCGAACATGCTCAGAACAACCGGTAAGAACACCAGC 7 0.17500000000000002 No Hit GTAGAGATCAACGATGGAGAGAAGATCGTCATCTTCAACGAGCTTGTCAA 7 0.17500000000000002 No Hit GCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCC 7 0.17500000000000002 No Hit AGTCAACAAAGTCAAGAACAGCAGCTAAATCCAATGCACCGCCAAATCTT 7 0.17500000000000002 No Hit GGCCCTGTAGACGGCGGTGGCAGAAGGAATGGTTAGTGTGAACACGTAGA 7 0.17500000000000002 No Hit GGTGTCGCTGCTCTCCACCTCCAGTGTAATCGTCTTCCCTGTCAAGGTCT 7 0.17500000000000002 No Hit GTCGGTGTATCAGCGCTTGGCGGTGAAGACTTTACAGGCACTAGCGATGG 7 0.17500000000000002 No Hit CTTTTTTTCATGATTGTAATGTCAGCTGACCTCTGGCTGTAGCAGAGGAA 7 0.17500000000000002 No Hit GGGAGAGGGAGGGTTAAGTCAGGGCGGCGACGCGGTCTGCTGTTGTTGGT 7 0.17500000000000002 No Hit GGTAAATCAACCTTGATCTCATCAACATCGAGCCAAAGAAAGAACTTTTG 6 0.15 No Hit ACACCATATGCTAGTACAACACTCTTCGAATTTAACAGCAACGGGAACTT 6 0.15 No Hit ATTGAAACAACGTTGTTCAAGAACCTCAGGCATCTGAATTTCTCCTAGCT 6 0.15 No Hit GTCGTAATCTTGAATTGCTTTCATGTGTTGTTGTGCTTTAGAATACGTCT 6 0.15 No Hit GGTCTTTGGATCTGCTGCTGCTATCTTTACCTTCAGAAGCCAATGCATCT 6 0.15 No Hit CATTGCCCTCTCCACGTTGACCACACAAGGAATCTATTTCATCAACGAAA 6 0.15 No Hit GCCCAGAATAACCGCCTCCATGTAAACAAAAAACAACCGGCCCCTCTGTC 6 0.15 No Hit GGGGAGGGTTGCACTCCTGGTGCAGGTAAGAGAGACCTTCAGCAGCTCCT 6 0.15 No Hit GCTGCTGATTGTAAGGATTAGCTTGCCCCATTCCTGGATAATATCCACCA 6 0.15 No Hit GCCAATTTGCAGCATCTGTGACTAGCTCAAGATCAAAAGAATTAACATTG 6 0.15 No Hit CAGCCCAAATGGCCCACAGGAGAAGCTGAGCAACTGCCATCACAACACAA 6 0.15 No Hit CCCGTAAGCTGTCAAGTGCTTCCGAGCTTCCTTGGTAATGGTCCGGCCAC 6 0.15 No Hit ACCCCTTAGCCTTTTCCTCAAGCTCCTCCTCCAATTGCTTTAGATGTTCC 6 0.15 No Hit GTGGAAGTGGGAGGAGGTGGTGGAGGTGAAGGGAGGGCAGGGAAAAAGGG 6 0.15 No Hit CCTTAACTGACTTAACAAGCCTGTCCACGGTATCATCAATCTCTTCAAAG 6 0.15 No Hit CTTTTCCTTTTCCTCAAGCTCCTCCTCCAATTGCTTTAGATGTTCCTCGG 6 0.15 No Hit CAACATCTGAACACTTGGACAATCCATCTACCACACCTACATCCTCTTCA 6 0.15 No Hit CGTAGATTTCGTTGCGGATTGGAATCAGGTTGTCGGGTTGGGAGGAGGAG 6 0.15 No Hit ACCACGTTCTGCCTCATCTGCCCGGGTATCAGTCATTCGAACATCACCAG 6 0.15 No Hit CCCATGTTTACTGACAGTTTTACCATCAGGACCTAAAATTACCAAGCAAG 6 0.15 No Hit CACTTCCATACTCAGCTCCAGCCAAAACAATGGTGTCAAGTCCAGCGTTC 6 0.15 No Hit GCACCATGTGGCAAAACCTACTACTATTCGTGCACAGCTGCAAGAGAGCA 6 0.15 No Hit TTATCATGAATATAAATGCCTACGTCTCTACATAAGGCTCCGCAATCGCA 5 0.125 No Hit CTTCAACACACCCTCTTGTCCTTTCGAAGCAAAAGTAACCCCATAATTCA 5 0.125 No Hit GTCACAATTGTGTGAATCATTGTACAAGGCAATTCTCCAAGCTTATGGGG 5 0.125 No Hit CACTGACAGGAAGCTGTATGTTTCACTAGCTACTGGTTTGGAACCTGATA 5 0.125 No Hit TTGGAAAGAATGATAATTGGGAGTTGAATGGTTTAGCCTCATCCGATCAT 5 0.125 No Hit AACCACTGCTTCTGCCACCACCAAAACGACCACCACCACTGCTTCTGCCG 5 0.125 No Hit AGCAATATAAAGTAACTTGAGCTTATTCTCTACTGGATCTGAGCCCACTT 5 0.125 No Hit GTTTATTTCTGCTCACAAGATAACATTTCTCCCTAGTTACAGAGACCAAA 5 0.125 No Hit CCCATCATGAACTTTATCCTCATCATTTGATGAGTTCCAGGGCATGGAGT 5 0.125 No Hit CACAAATACAAGTGAGAGAAGCTCAAGCCAGAGTAAGATACATGAATAAA 5 0.125 No Hit GGGATAAACTTGTTCATACACGAAGGAAGAGTTGGGGAAGGCTTTAACAG 5 0.125 No Hit GTCGAATTAGAGGAAGAACAGGAGCATAGACAAGAGGAACCAAGAACTTG 5 0.125 No Hit ATCAGCTTTAACAGTATCTATTGCTTTTCCCATTGAATACTCAGGTGCCA 5 0.125 No Hit GTCTGGATGTGAAAGCTTCAGTTGGCTGAGATAACGTGAAGAAGATTTGC 5 0.125 No Hit CTAACTTTAAGACCCCTCTCCTTCCATTGACGTAAGCATGCATCAATCTG 5 0.125 No Hit GATCAGTCCAGATTAGCAATGACAGCATCAACCACCTCTTGTGTGGTGCT 5 0.125 No Hit CCTCAACCCATATTTGCTGCAAGTAGTGGCTAGGGTCATATTAGTGTTAA 5 0.125 No Hit CACCCTTGAATCAATCTCTCTTGTCTCTTGATCAGCTCATCCTTTACTTT 5 0.125 No Hit CATAGCATTAGTTATACCAAGTGGAGCAGCTGACTTCACTGGCTCAAAAC 5 0.125 No Hit CGAAAACAGAGATTCTAAAAATGTTTTTTAGTATAATGGGGACACGAAAA 5 0.125 No Hit CTTTCTTTCAGTATAATGCCCCCGCTGAATAATCCTATCAAAGAGTTCAC 5 0.125 No Hit CTTGTGTACATCTTAGTTTATGGGAAAGGACTAAAGGAGAGGAGATGGTT 5 0.125 No Hit GTGACATCTACAATTCCACGTATGTTTCCTATTATACCATAGCAGACAGG 5 0.125 No Hit CGTGGCTTCTGGTTTTGGGAGCCAAAAATCCCAATGGCAGGGTGGCGGCT 5 0.125 No Hit CAGCAGACCCAAAATCTTTACTGGCTATGACCTTCCAAACAACTGTTTCC 5 0.125 No Hit GAGGCAAGCAATCCAGGACACGTGGGATTAACAGCACATGCCCATATTCA 5 0.125 No Hit CCGTAAGCTGTCAAGTGCATCCGAGCTTCCTTGGTAATGGTCCGGCCACT 5 0.125 No Hit GGTATCAGGGGGTCCAATTATTGAAACGCTCCATTCAAACATGTCGGTTT 5 0.125 No Hit GCCATCATGATTGCATGGGTTGTAGAACCCAGCAGTTAATTCAGCAGCAT 5 0.125 No Hit CTCATCAACAGGACCTAGGAGGTCAATTCCAGGTTTCAATTCAGATTCAG 5 0.125 No Hit GAGATCTTCAACATGAAAAGCAGATTCATCGTCATCTTCAGCTACCATCC 5 0.125 No Hit GTTCTTGTTGTTTTACCATCTTTCTTCAAACCTTTCTCAACAGTTATCTC 5 0.125 No Hit GCTTGAAATGCCTTCCGTATCACTTCCACTGACATCGATTGTTGCTCCGA 5 0.125 No Hit CTCAACTGGATCTAGCCTACATCCTCATCTCGCCCTCGCTTTCCAGCAGG 5 0.125 No Hit CTACACAATTACCATGGAACCTATGCTTACAAGGCATCTCCTTTACTACC 5 0.125 No Hit CCCAGGCGGCCACGCCGTTTCATCAGCGCCGAGACAAGAGTCGGCGGTGA 5 0.125 No Hit CCATGGTAATCACCAGGAGGTAAGGATTTCCTTCCAAGAACACCCTCTTC 5 0.125 No Hit CCCCAATGCTATACCCTTGTGCTCTTAGCTTCAGGGGGAATATCTCCGAG 5 0.125 No Hit GCACCAGGAGTATTGACAACATCATAAAGATGAAGAACAGAGACCAAAGG 5 0.125 No Hit GTATCGTACAATGTCCCCCATCCATGATAGCTTTTGCCTTTGGAACTTCC 5 0.125 No Hit TTCATAATGTGGGCCACACTGTTGGTGTAAATCTTCTCACGAGCTGAACG 5 0.125 No Hit ATAACATCTGTCTTGCCAGCCTCTGGAATGGTGCTTGCAGATGTGCTCTT 5 0.125 No Hit CTATATAAAAATGCTTCATTCTAGCCACTTGAAGATCCCTCGTGCAGTTC 5 0.125 No Hit GTGGCGAAGAAGCTTAATCTCTCGTAAAGTCCTCTTGGCATCTATTCTGT 5 0.125 No Hit CTCTGCACTATTTTGAGAACCTTTACAGTCATGAACACCACCAAGCTTCA 5 0.125 No Hit GCCATCTTTCAGGTTTGTATTCCTCAGCATCATCGCCCCATATGAACTTC 5 0.125 No Hit ATCCTTTCTTTGTTGAAGTTTCAGGAACAGATCCTCTCTTAGTGATATTC 5 0.125 No Hit ATTCTGATGGCCGACCAGCCTTCTTTAACAGCTGAATGGTTGCTGTCAGA 5 0.125 No Hit CCCCTGTTCCTCCAAGCGTAACATGATGCAAAATGGAGACATTGTTCCCA 5 0.125 No Hit CTCGGATAAAACTAGAAAGAAAAGAAAAGGGAACTGTAACCATTGAAATG 5 0.125 No Hit GACCTGATGGGTTTCAGAGATGGACATCCAAACACTCAAGCACATCATGC 5 0.125 No Hit CAATTGTCCGGTCATGGCATACTCAGGAGCACAATAACCATAAGTACCCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.16249999999999998 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.30000000000000004 0.0 0.0 0.0 0.0 86-87 0.4125 0.0 0.0 0.0 0.0 88-89 0.425 0.0 0.0 0.0 0.0 90-91 0.525 0.0 0.0 0.0 0.0 92-93 0.6000000000000001 0.0 0.0 0.0 0.0 94-95 0.7375 0.0 0.0 0.0 0.0 96-97 0.95 0.0 0.0 0.0 0.0 98-99 1.1749999999999998 0.0 0.0 0.0 0.0 100-101 1.45 0.0 0.0 0.0 0.0 102-103 1.8625 0.0 0.0 0.0 0.0 104-105 2.175 0.0 0.0 0.0 0.0 106-107 2.5 0.0 0.0 0.0 0.0 108-109 2.7625 0.0 0.0 0.0 0.0 110-111 3.0625 0.0 0.0 0.0 0.0 112-113 3.4124999999999996 0.0 0.0 0.0 0.0 114-115 3.8125 0.0 0.0 0.0 0.0 116-117 4.25 0.0 0.0 0.0 0.0 118-119 4.7875 0.0 0.0 0.0 0.0 120-121 5.325 0.0 0.0 0.0 0.0 122-123 5.95 0.0 0.0 0.0 0.0 124-125 6.4 0.0 0.0 0.0 0.0 126-127 7.1875 0.0 0.0 0.0 0.0 128-129 7.824999999999999 0.0 0.0 0.0 0.0 130-131 8.412500000000001 0.0 0.0 0.0 0.0 132-133 8.8125 0.0 0.0 0.0 0.0 134-135 9.2125 0.0 0.0 0.0 0.0 136-137 9.837499999999999 0.0 0.0 0.0 0.0 138-139 10.825 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATGGT 10 0.006830828 145.0 1 GTGTCGC 10 0.006830828 145.0 2 TAGGATG 10 0.006830828 145.0 6 CTTTAGG 10 0.006830828 145.0 3 GTCGCTG 10 0.006830828 145.0 4 TGGTAAG 10 0.006830828 145.0 4 GATGTTA 10 0.006830828 145.0 9 ATGGTAA 10 0.006830828 145.0 3 TGCCTGC 10 0.006830828 145.0 145 GCTGCTC 10 0.006830828 145.0 7 TCGCTGC 10 0.006830828 145.0 5 AGGCCAA 10 0.006830828 145.0 9 AAGGCCA 10 0.006830828 145.0 8 GTAAGGC 10 0.006830828 145.0 6 TTAGGAT 10 0.006830828 145.0 5 CTGCTCT 10 0.006830828 145.0 8 TCTCACA 10 0.006830828 145.0 145 GCTTTAG 10 0.006830828 145.0 2 TGTCGCT 10 0.006830828 145.0 3 TATGGTA 10 0.006830828 145.0 2 >>END_MODULE SRR26075320 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075320_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.85825 37.0 37.0 37.0 37.0 37.0 2 36.108 37.0 37.0 37.0 37.0 37.0 3 36.1795 37.0 37.0 37.0 37.0 37.0 4 36.1255 37.0 37.0 37.0 37.0 37.0 5 36.1665 37.0 37.0 37.0 37.0 37.0 6 36.0805 37.0 37.0 37.0 37.0 37.0 7 36.0765 37.0 37.0 37.0 37.0 37.0 8 36.16 37.0 37.0 37.0 37.0 37.0 9 36.0475 37.0 37.0 37.0 37.0 37.0 10-14 36.0631 37.0 37.0 37.0 37.0 37.0 15-19 36.0145 37.0 37.0 37.0 37.0 37.0 20-24 35.9471 37.0 37.0 37.0 37.0 37.0 25-29 35.8806 37.0 37.0 37.0 37.0 37.0 30-34 35.7832 37.0 37.0 37.0 37.0 37.0 35-39 35.707 37.0 37.0 37.0 37.0 37.0 40-44 35.7181 37.0 37.0 37.0 37.0 37.0 45-49 35.6272 37.0 37.0 37.0 37.0 37.0 50-54 35.5222 37.0 37.0 37.0 37.0 37.0 55-59 35.5891 37.0 37.0 37.0 37.0 37.0 60-64 35.6693 37.0 37.0 37.0 37.0 37.0 65-69 35.5232 37.0 37.0 37.0 37.0 37.0 70-74 35.4568 37.0 37.0 37.0 37.0 37.0 75-79 35.3852 37.0 37.0 37.0 37.0 37.0 80-84 35.4895 37.0 37.0 37.0 37.0 37.0 85-89 35.384699999999995 37.0 37.0 37.0 37.0 37.0 90-94 35.374 37.0 37.0 37.0 34.6 37.0 95-99 35.3523 37.0 37.0 37.0 37.0 37.0 100-104 35.255199999999995 37.0 37.0 37.0 34.6 37.0 105-109 35.263400000000004 37.0 37.0 37.0 29.8 37.0 110-114 35.190200000000004 37.0 37.0 37.0 32.2 37.0 115-119 35.182900000000004 37.0 37.0 37.0 29.8 37.0 120-124 35.0244 37.0 37.0 37.0 25.0 37.0 125-129 34.944100000000006 37.0 37.0 37.0 25.0 37.0 130-134 34.907300000000006 37.0 37.0 37.0 25.0 37.0 135-139 34.79685 37.0 37.0 37.0 25.0 37.0 140-144 34.79275 37.0 37.0 37.0 25.0 37.0 145-149 34.67685 37.0 37.0 37.0 25.0 37.0 150-151 34.343374999999995 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 8.0 15 8.0 16 8.0 17 2.0 18 2.0 19 2.0 20 2.0 21 2.0 22 8.0 23 15.0 24 13.0 25 27.0 26 19.0 27 18.0 28 18.0 29 35.0 30 26.0 31 55.0 32 71.0 33 107.0 34 222.0 35 762.0 36 2424.0 37 144.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.886971742935735 20.555138784696176 10.127531882970743 21.43035758939735 2 29.849999999999998 25.7 25.724999999999998 18.725 3 23.05 29.275000000000002 30.5 17.175 4 27.425 35.949999999999996 20.0 16.625 5 26.375 34.425 22.45 16.75 6 23.3 38.4 21.0 17.299999999999997 7 21.5 21.5 36.475 20.525 8 23.1 27.05 26.625 23.225 9 24.025 25.275 27.700000000000003 23.0 10-14 24.93 29.815 24.605 20.65 15-19 24.89 28.810000000000002 26.284999999999997 20.015 20-24 24.205 28.32 26.400000000000002 21.075 25-29 24.945 29.830000000000002 25.61 19.615 30-34 25.230000000000004 28.59 25.97 20.21 35-39 24.755 28.470000000000002 26.505000000000003 20.27 40-44 23.794999999999998 28.84 26.575 20.79 45-49 25.005 27.6 26.75 20.645 50-54 23.87 28.07 26.979999999999997 21.08 55-59 24.42 29.34 25.905 20.335 60-64 25.669999999999998 27.255000000000003 26.924999999999997 20.150000000000002 65-69 24.060000000000002 27.125 27.279999999999998 21.535 70-74 23.97 28.439999999999998 27.13 20.46 75-79 24.065 28.720000000000002 26.815 20.4 80-84 24.73 27.834999999999997 26.465 20.97 85-89 24.92 28.315 26.334999999999997 20.43 90-94 24.585 28.23 26.05 21.135 95-99 24.515 28.610000000000003 26.5 20.375 100-104 25.009999999999998 27.889999999999997 27.425 19.675 105-109 24.8 27.384999999999998 27.425 20.39 110-114 24.85 28.29 26.525 20.335 115-119 25.095 27.735 26.855 20.315 120-124 24.665 29.225 26.615 19.495 125-129 26.279999999999998 28.48 25.365 19.875 130-134 26.650000000000002 27.634999999999998 26.27 19.445 135-139 26.146307315365767 28.491424571228563 26.626331316565828 18.73593679683984 140-144 26.238935840376055 27.729159373906086 26.133920088013202 19.897984697704658 145-149 26.03650912728182 28.392098024506122 25.726431607901972 19.844961240310077 150-151 25.828228528566072 28.403550443805475 26.190773846730842 19.57744718089761 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.0 16 1.0 17 3.0 18 2.5 19 0.5 20 2.0 21 3.0 22 1.0 23 1.5 24 4.0 25 3.5 26 2.5 27 2.5 28 1.5 29 5.0 30 10.5 31 10.0 32 17.5 33 21.0 34 22.5 35 43.0 36 61.5 37 75.0 38 93.0 39 136.5 40 191.5 41 226.0 42 270.5 43 288.0 44 287.0 45 300.0 46 280.5 47 253.5 48 254.0 49 211.0 50 145.0 51 143.5 52 144.0 53 118.5 54 81.0 55 52.0 56 42.0 57 28.5 58 19.0 59 18.0 60 16.0 61 14.5 62 14.0 63 12.0 64 8.5 65 3.0 66 2.0 67 1.5 68 0.5 69 3.5 70 3.0 71 0.5 72 3.0 73 3.0 74 0.5 75 0.5 76 1.0 77 1.0 78 0.5 79 1.0 80 1.5 81 0.5 82 0.0 83 0.5 84 1.5 85 2.5 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 1.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.005 140-144 0.015 145-149 0.025 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.425 #Duplication Level Percentage of deduplicated Percentage of total 1 63.79809681423252 38.550000000000004 2 20.521307405875053 24.8 3 8.647083160943318 15.675 4 3.392635498551924 8.200000000000001 5 2.110053785684733 6.375 6 0.9515928837401738 3.45 7 0.33098882912701694 1.4000000000000001 8 0.16549441456350847 0.8 9 0.0 0.0 >10 0.08274720728175423 0.75 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 20 0.5 No Hit GCTTCAGCAAATTCATTTTCCAAGTAGCCCTTTATCCTGTTTTTCCTATA 10 0.25 No Hit GGTACCTGCTGAGGCTAGGGTTTGTTTGTCACAGAGCCATAGTTTTAAAG 8 0.2 No Hit GCCCCGATGTCGTTAGTTTGCAGTTTACGGTGGGTCGTTTTACTAGGTTC 8 0.2 No Hit CGGAGCTGAGAGAGAAGACAGGCAAGCCGTGATTGATGATGTGTTAAAAC 8 0.2 No Hit CGGAGTCATCTATTTTGGGAATGCTGGAAGCTTGGATAAGAAAACAATGG 8 0.2 No Hit GTGCAGAACCGCCAGCCTCGTCTCAAGGACCATCATTGTCACCTATCTCT 7 0.17500000000000002 No Hit TCTGTCTTCAGTGGCATCACGTTAACAAAGCTAGTTGGCGTGATTGTTCT 7 0.17500000000000002 No Hit GACAAAGTTGGTGCTTTACTTCACCGGAGCCACCAACATCCTCTACACCT 7 0.17500000000000002 No Hit CTTTGATAACTCTAAAGGAGAAGTCTCTGATAAGCCTGCTATCAGCTCTG 7 0.17500000000000002 No Hit AGCTTGCTGATGACCCAAAACTCGCCGTTCAACGTTATGGCCAGTACTTA 7 0.17500000000000002 No Hit TGGCTATACAAGAAGCTAACAAGATTGAAAAAGGAACTGATGGCAGCCAA 7 0.17500000000000002 No Hit AGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTG 7 0.17500000000000002 No Hit GAAAATTAACATGTTCTCTCCAAGTGAAGCGCCCAAGGAAGTTTTCTGGC 7 0.17500000000000002 No Hit TACCAAGGAAGCTCGGATGCACTTGACAGCTTACGGGGCAGATGCTTTTC 6 0.15 No Hit GTGGGACCCAAGACTGTCCATATTCCTACAGGCGAGAAACTCTCTGTGTA 6 0.15 No Hit GCTTAGGAGTAGAAGAAACATCTTTCCTAGGACGAGGACGAGGGCGATAT 6 0.15 No Hit GAATTAGAATTTTTCAGCAAGCATTCACATGCACGTTTAGTCCCTCTTTT 6 0.15 No Hit GTTCGGTCTTTGAAATCTTGCCAAAATTTGGCCTCCCAAGTGGTCTATTA 6 0.15 No Hit TGTAGAAGCTGGTGCTGTTAAACATCTTGTGGACTTGATGGACCCGGCAG 6 0.15 No Hit GCAGCCAATAATGGCAGCGCAGGTGGAAATGGAAACAGTAATGGCAATGG 6 0.15 No Hit CCTAGACGTAGAAGACGAACAGCAGCAGCAAGAAAATCTAAAAAATCCAC 6 0.15 No Hit GGAAGCGAAAGCTTAAGAAAGACCAGGAAAGAAACAAACTTGAAGCAGAG 6 0.15 No Hit TTTGTGCTCGGGACGTGCTTGGGGCTGCGATGAGGCTGTTAAGTATCCGG 6 0.15 No Hit GAAATTCAAAATTCAAGGCATTCCTGCAGCTGTAGCGATTGGCCCAAGTG 6 0.15 No Hit GAAGCTGCAAGGGTCATGGTTGCCGCACCACTACTTGCTTTTCTGACTAC 6 0.15 No Hit CCACTGTCCAGCCCCCCTCCTCTCATGGCTTCACCGTCTGATCCCACCAA 6 0.15 No Hit CTCAGACCTTTACTCTTCGACAGCTTAAAATCTCTACCCACCAGCACTGG 6 0.15 No Hit AAAAAATCATAAACTTTCAGTGATGGCTTCTCGTCTAACAACCACCGCCC 6 0.15 No Hit GGGTTGTTTCCCTGCTGCTGGTTCAGAAGAAGATGCTCAGAAGCTTATAT 6 0.15 No Hit GTTGATGGAAGGAAACAAATCCTGAGTCGGAAATTCAAAATTCAAGGCAT 6 0.15 No Hit GGAAAGTCATACTTGGCCAAGGCTGTTGCAACTGAAGCTGAGTCCACTTT 6 0.15 No Hit TGTGTTTGTGTCAAGTGATCGCGATCAAGCTGAATTCGACTCCTACTTCA 6 0.15 No Hit TGTCCACCACCAAAACCATGATCCCTCTCCTCCTCCTCCTCCTCCTCCTC 6 0.15 No Hit CACTGGTGGAACCAAAGGGATCGGATATGCTGTTGTGGAAGAATTGGCAG 6 0.15 No Hit CTCTCTTATTCCTTCTCGTCCTTCTGTTCACCACCACGACCTCCACCACT 6 0.15 No Hit CAAGGCCTCATGCACCGCCACAACATACCCTGCCTTGTGTGTGCAATCCC 6 0.15 No Hit GAAAGATTCATGTCCTCACAAAAGACGCCAATCAGAACATTGCAGTTATG 5 0.125 No Hit GCCAGAACCAAAGCCAAAGCCAAAGAACAAGCCTGGGAGGCTTCGCAGAC 5 0.125 No Hit CCACAATGTTGCTCCAAAGGGAAAATTCATTGCATTTGTATCAACCGAGG 5 0.125 No Hit GTGTTCTGAAAGAGTGTGAAGAGTCAGGGTTTCATCCTCATGGAGAACCA 5 0.125 No Hit ACCAAATCTGTACTGTTACCGACCGAACAAGGAAAAAAAAAAACAGAGAG 5 0.125 No Hit CTTGTGAATAGCAGGCGGTCCTCTATTCCCTTCAGTCGACCCTCTATGTG 5 0.125 No Hit GTTCATGGAAACATAAATCCTAGTAATATTATGCTTGATAACTCTCTTAC 5 0.125 No Hit ATTTGGGTTCCAGCTCCTCAATTGAGGAGAAGAGCTATGACAAAGCATCT 5 0.125 No Hit CTTCAATGGCTCGGTCAGGTTACACAAGCGATCTAGTTGGCTCAGTATTT 5 0.125 No Hit AGGGGAAACTCTTCCTTTCCTTCAGCCGACCACAGACCCCCTCAGCCCCG 5 0.125 No Hit CAGAGAGCTCCAGGCCATTGACCGCTCTCCCCATCCTTCTCATCAGATTG 5 0.125 No Hit CAGGTGGTGGATCAGCAGAAGATCCTGAGGATGACTCACACGATGAACTC 5 0.125 No Hit GGCAATTTGCTAAACGAAGAATATATATATATATAAAACCCTCTCTCTTG 5 0.125 No Hit ACTAAATATCTGCGAAAATTTATTCCTTAACAGGGAAGAAACAGACACAG 5 0.125 No Hit GCCAAGGATGAATGCTGCCCACTGCTGGAAGGTCTTGTAGACTTGGATGC 5 0.125 No Hit CTGAGACACGTGAAGAGGTTGCCATCAAGAAGATTGGTAATGCATTTGAC 5 0.125 No Hit GATACATTTGGTGTACCATCACAAATCCTGTCATTGTCTAAGATGTCAAG 5 0.125 No Hit GCTGGAGGAATTGGCCAGCCTCTGGCGATGCTTATGAAGATGAATCCTTT 5 0.125 No Hit CTTGTGGGAAGCTACAGTCTTCTCCCCAAAGAATTGATATGGCTTTGTTG 5 0.125 No Hit GTCAAATCAGTGCCAGGAGGGGATTCATCTCTGGGGTACCTGTTTGGAGA 5 0.125 No Hit TACAAAGGTTTCTTGGCTTGCCAGGCTCATCGAGTTGCTCACAAGTTGTG 5 0.125 No Hit CTTTGATGAAGCTGAAACACTGTTGCTTGAAGCACTAAACAAGGATGCAA 5 0.125 No Hit GGGTATTATGGCAGGTTCTTCCTTAATTGGTATGCTAGGATTTTAGTTGA 5 0.125 No Hit ATTTGATGACTGCGGCTGCAGTCCAAGCTCTTTTGGGGTAAAAAGAAAAT 5 0.125 No Hit GCTTACAATATGGGAGTGACACAAGAAAGAAAGAGATGAAATGGGTTGGG 5 0.125 No Hit GGAACACATGATGCTATAAGCAATCAACTTTGTAGATAATTTACAGACGG 5 0.125 No Hit TGTTTTTGCTCGTGTAGGTGATGATATTCAGTGGCCTCTGGCTAATGATG 5 0.125 No Hit ACTGGATGTACAGTGGAGAGACGAAGAGCGAGCATCGTTGATGAGGTTGC 5 0.125 No Hit GTGGGGAGAAGGCGCCTTCTTCTTGTTAGCACAGCAGGTATCATAGCAGC 5 0.125 No Hit CTTCTATTATCTTGTTCAGGACTTGAAGTGCTTTGTTTTCTCTCTCATCT 5 0.125 No Hit CAAATCCAGTGGCTTTGCTCCTCTCGTCTGCCATGATGCTGAGACACCTT 5 0.125 No Hit TGGAGGTATCAGAGTGGCTGATCCATCTACTGTAGTTGACATGACAGGAA 5 0.125 No Hit TGCAAATCGATTGCTAAGAGGAAGTTGTTAACTGATGAGGATGTGGAGGA 5 0.125 No Hit CAAGAACCACAAACAACAGACCCAAACGAAAGAATCATCCTTATAAACCC 5 0.125 No Hit GCCGGACTCTGGCTGACTACAATATCCAAAAAGAGTCTACCCTTCACCTT 5 0.125 No Hit GAATCTCCTCCTCTCCACAAGTGACGGTAATGCAGTTCTGAAAATTGCAG 5 0.125 No Hit GCTAATTGAAGCATACCACGCAATTAAAGCAAAAGACAATGCATTTGAGG 5 0.125 No Hit TGTTTCTTGATTTACACTCTGTTTTCACTGCTTGTTATATTTTAGCAGTG 5 0.125 No Hit ACTATCTCCATGCAGCAATTTCTGAAACTCTTAGACTGTATCCATCTGTG 5 0.125 No Hit GGACTTCTTCTACTGTGTCTTATATTGGTGGGGTTTGAGTAGCTACTAAC 5 0.125 No Hit GAGGAACACCTGCATCTCACACAGAGAGACCAAAACAATGACAACTTCGA 5 0.125 No Hit AGCAAAACCAAGGACTGATAACCGTGACAGTCGGGATAGTGGCAGAGGTG 5 0.125 No Hit GGTCCTCCAGCCCTTCGATGGAAACAAATTTAACTTCACTAAAGTTGGTC 5 0.125 No Hit CAGAAGAGTTCAATGACTGCTCCAAACAAGTAATTGAAATGGAGTCCCTA 5 0.125 No Hit AAAGGATCCCCCCTCTCCCTCTCAAAATTCCCCAGCCCATCCTATTCGAC 5 0.125 No Hit GCTGCATGAGGCGAGACTACTTAGCTGCAATGGATCATTATATTAGACTG 5 0.125 No Hit GGTACAAAGATCATGGGCAGCACGTAAATGGTGAATTTTCTATGGCAGTA 5 0.125 No Hit AGACAGTGACAGAATTGACACTTAATTTGGAGGTGGGTCCAATAAGCATG 5 0.125 No Hit TTATAAGACAGCAGCGAGTGGATTCTCTGCCAAGCTGACACCACAACAAG 5 0.125 No Hit GGGAATCCTGGCATTGAAACAATAACCAACTGGTTCAAGATTGAGAAGGC 5 0.125 No Hit TGTAGCATTATGTGAAAGAAACCAATGATGCATTTTATCACCAGCCAAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.2875 0.0 0.0 0.0 0.0 86-87 0.4125 0.0 0.0 0.0 0.0 88-89 0.425 0.0 0.0 0.0 0.0 90-91 0.5375 0.0 0.0 0.0 0.0 92-93 0.625 0.0 0.0 0.0 0.0 94-95 0.7625 0.0 0.0 0.0 0.0 96-97 0.975 0.0 0.0 0.0 0.0 98-99 1.0750000000000002 0.0 0.0 0.0 0.0 100-101 1.35 0.0 0.0 0.0 0.0 102-103 1.75 0.0 0.0 0.0 0.0 104-105 2.075 0.0 0.0 0.0 0.0 106-107 2.4000000000000004 0.0 0.0 0.0 0.0 108-109 2.675 0.0 0.0 0.0 0.0 110-111 2.9875 0.0 0.0 0.0 0.0 112-113 3.4000000000000004 0.0 0.0 0.0 0.0 114-115 3.8625 0.0 0.0 0.0 0.0 116-117 4.3 0.0 0.0 0.0 0.0 118-119 4.85 0.0 0.0 0.0 0.0 120-121 5.4 0.0 0.0 0.0 0.0 122-123 6.025 0.0 0.0 0.0 0.0 124-125 6.449999999999999 0.0 0.0 0.0 0.0 126-127 7.262499999999999 0.0 0.0 0.0 0.0 128-129 7.925 0.0 0.0 0.0 0.0 130-131 8.5125 0.0 0.0 0.0 0.0 132-133 8.9125 0.0 0.0 0.0 0.0 134-135 9.3125 0.0 0.0 0.0 0.0 136-137 9.975 0.0 0.0 0.0 0.0 138-139 10.9875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCTGGC 10 0.006830828 145.0 7 CCGGACT 10 0.006830828 145.0 2 CGGAGCT 10 0.006830828 145.0 1 AGTAGCT 10 0.006830828 145.0 1 GGACTCT 10 0.006830828 145.0 4 GAGCTGA 10 0.006830828 145.0 3 CGGACTC 10 0.006830828 145.0 3 GCCGGAC 10 0.006830828 145.0 1 CTGGAAA 10 0.006830828 145.0 6 GACTCTG 10 0.006830828 145.0 5 >>END_MODULE Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra Read 1178262 spots for SRR26075320.sra Written 1178262 spots for SRR26075320.sra Read 1178259 spots for SRR26075320.sra Written 1178259 spots for SRR26075320.sra SRR ids: ['SRR26075320.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_5fp0zdit SRR26075320.sra spots: 23565183 blocks: [[1, 1178259], [1178260, 2356518], [2356519, 3534777], [3534778, 4713036], [4713037, 5891295], [5891296, 7069554], [7069555, 8247813], [8247814, 9426072], [9426073, 10604331], [10604332, 11782590], [11782591, 12960849], [12960850, 14139108], [14139109, 15317367], [15317368, 16495626], [16495627, 17673885], [17673886, 18852144], [18852145, 20030403], [20030404, 21208662], [21208663, 22386921], [22386922, 23565183]] SRR26075320 file size 8698722 SRR26075320 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075320 SRR26075320_1.fastq SRR26075320_2.fastq Input file: SRR26075320_1.fastq Paired file: SRR26075320_2.fastq trimmed: SRR26075320-trimmed-pair1.fastq, SRR26075320-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:00:49 2025 >> started Wed Feb 12 02:01:28 2025 >> done (39.320s) 23565183 read pairs processed; of these: 127 ( 0.00%) short read pairs filtered out after trimming by size control 64984 ( 0.28%) empty read pairs filtered out after trimming by size control 23500072 (99.72%) read pairs available; of these: 3260276 (13.87%) trimmed read pairs available after processing 20239796 (86.13%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 8 0.00% 20 17 0.00% 21 29 0.00% 22 27 0.00% 23 14 0.00% 24 14 0.00% 25 36 0.00% 26 32 0.00% 27 28 0.00% 28 33 0.00% 29 40 0.00% 30 44 0.00% 31 16 0.00% 32 32 0.00% 33 44 0.00% 34 41 0.00% 35 41 0.00% 36 37 0.00% 37 47 0.00% 38 54 0.00% 39 60 0.00% 40 64 0.00% 41 64 0.00% 42 74 0.00% 43 62 0.00% 44 61 0.00% 45 76 0.00% 46 74 0.00% 47 87 0.00% 48 105 0.00% 49 93 0.00% 50 137 0.00% 51 143 0.00% 52 193 0.00% 53 167 0.00% 54 201 0.00% 55 205 0.00% 56 205 0.00% 57 235 0.00% 58 262 0.00% 59 282 0.00% 60 395 0.00% 61 427 0.00% 62 402 0.00% 63 508 0.00% 64 560 0.00% 65 630 0.00% 66 675 0.00% 67 822 0.00% 68 899 0.00% 69 969 0.00% 70 1197 0.01% 71 1390 0.01% 72 1555 0.01% 73 1805 0.01% 74 1991 0.01% 75 2200 0.01% 76 2654 0.01% 77 2822 0.01% 78 3212 0.01% 79 3612 0.02% 80 4006 0.02% 81 4581 0.02% 82 5099 0.02% 83 6181 0.03% 84 6700 0.03% 85 7251 0.03% 86 7714 0.03% 87 8739 0.04% 88 9030 0.04% 89 9968 0.04% 90 10871 0.05% 91 12062 0.05% 92 13067 0.06% 93 14405 0.06% 94 15849 0.07% 95 16493 0.07% 96 18055 0.08% 97 18506 0.08% 98 19908 0.08% 99 21210 0.09% 100 22251 0.09% 101 23132 0.10% 102 24941 0.11% 103 26852 0.11% 104 28292 0.12% 105 29718 0.13% 106 30679 0.13% 107 32280 0.14% 108 32948 0.14% 109 34683 0.15% 110 35257 0.15% 111 36852 0.16% 112 38551 0.16% 113 39805 0.17% 114 42077 0.18% 115 44015 0.19% 116 46035 0.20% 117 46608 0.20% 118 48432 0.21% 119 48977 0.21% 120 50032 0.21% 121 50434 0.21% 122 53184 0.23% 123 55465 0.24% 124 57383 0.24% 125 58202 0.25% 126 60863 0.26% 127 62834 0.27% 128 63884 0.27% 129 64996 0.28% 130 66327 0.28% 131 66631 0.28% 132 68702 0.29% 133 70660 0.30% 134 71880 0.31% 135 74128 0.32% 136 76636 0.33% 137 76742 0.33% 138 78951 0.34% 139 79879 0.34% 140 81508 0.35% 141 81842 0.35% 142 84012 0.36% 143 84811 0.36% 144 88390 0.38% 145 90264 0.38% 146 91341 0.39% 147 92103 0.39% 148 93527 0.40% 149 94910 0.40% 150 95462 0.41% 151 20239796 86.13% 23500072 reads passed initial QC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=4.57 fanout-score-rank=20 prefix-density=0.37 prefix-fanout=3.3 sequence=TCCACACTTGCAGCCATTCTCAGCACCAAAGTTCATCTCAGACCTCTCATAGAACAT criterion=fanout-score sequence-density=0.04 sequence-density-rank=38 fanout-score=124.98 fanout-score-rank=1 prefix-density=0.38 prefix-fanout=12.0 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGT criterion=sequence-density sequence-density=0.47 sequence-density-rank=1 fanout-score=2.64 fanout-score-rank=28 prefix-density=0.48 prefix-fanout=2.6 sequence=ATGTACCCTGACTTAGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=181.91 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=8.2 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAAAGATCAGCACCAGACCACTTAACATCAAAGATATTGACGTCGGAGAGGGGAGCAGTCCTGTTAATAAGTTTCAGAGGTCCATGACTATGCCAGGAACTCCAGGGACACCGACGACACCAGTGACCCCTACAACCCCAGTGTCGGCGCGTAGCAATGTTTGGAGGAGCGTGTTCCACCCTGGTAGCAACCTTGCTACTAAGAATATTGGTGCTCATGTTTTTGACAAGCCACAGCCTAACACACCCACTGTCTATGACTGGATGTACAGTGGAGAGACGAAGAGCGAGCATCGTTGATGAGGTTGCCTTCAACCAAGGTTGCCCATGTAAATACGTACTGTGTTTTGTTTTTCAGTACT SRR26075320 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:02:06 Started mapping on | Feb 12 02:02:07 Finished on | Feb 12 02:05:44 Mapping speed, Million of reads per hour | 389.86 Number of input reads | 23500072 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 21157891 Uniquely mapped reads % | 90.03% Average mapped length | 293.68 Number of splices: Total | 20156612 Number of splices: Annotated (sjdb) | 19653801 Number of splices: GT/AG | 19795288 Number of splices: GC/AG | 276763 Number of splices: AT/AC | 18776 Number of splices: Non-canonical | 65785 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 3.06 Insertion rate per base | 0.02% Insertion average length | 2.54 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 813022 % of reads mapped to multiple loci | 3.46% Number of reads mapped to too many loci | 69764 % of reads mapped to too many loci | 0.30% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.86% % of reads unmapped: other | 0.35% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1529159 1529159 1529159 N_multimapping 813022 813022 813022 N_noFeature 533403 20893114 667549 N_ambiguous 261255 1291 129834 UnstrandedReadsAssigned:20363233 PositiveStrandReadsAssigned:263486 NegativeStrandReadsAssigned:20360508 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075320 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075320-trimmed-pair1.fastq SRR26075320-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 23,500,072 reads, 20,758,134 reads pseudoaligned [quant] estimated average fragment length: 217.961 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,205 rounds 52401 SRR26075320.ke.tsv 34699 SRR26075320.se.tsv 87100 total ==> SRR26075320.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1801.04 2076 47.6718 Potri.005G024800.1.v4.1 1035 818.039 1247 63.0448 Potri.004G059700.1.v4.1 961 744.059 9 0.500256 Potri.007G009000.2.v4.1 1416 1199.04 0 0 Potri.003G141000.2.v4.1 2943 2726.04 1044 15.8389 Potri.016G087400.1.v4.1 270 86.6557 1502 716.852 Potri.015G069301.1.v4.1 564 349.104 0 0 Potri.010G195200.1.v4.1 1773 1556.04 273 7.25603 Potri.012G127500.1.v4.1 977 760.039 4417 240.352 ==> SRR26075320.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 746 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 383 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 165 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1050 SRR26075320 completed mapping pipeline successfully