Starting /dee2/code/volunteer_pipeline.sh SRR26075321 current disk space = 3053460946944 free memory = 1150983764 SRR26075321 SRAfilesize 9a34022244cc5f0e5022d97e9e4a5369 SRR26075321.sra SRR26075321.sra file validated SRR26075321 is paired end SRR26075321 is conventional basespace SRR26075321 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075321_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.58125 37.0 37.0 37.0 37.0 37.0 2 36.5775 37.0 37.0 37.0 37.0 37.0 3 36.688 37.0 37.0 37.0 37.0 37.0 4 36.7465 37.0 37.0 37.0 37.0 37.0 5 36.7535 37.0 37.0 37.0 37.0 37.0 6 36.7575 37.0 37.0 37.0 37.0 37.0 7 36.6855 37.0 37.0 37.0 37.0 37.0 8 36.664 37.0 37.0 37.0 37.0 37.0 9 36.6985 37.0 37.0 37.0 37.0 37.0 10-14 36.65535 37.0 37.0 37.0 37.0 37.0 15-19 36.6413 37.0 37.0 37.0 37.0 37.0 20-24 36.6192 37.0 37.0 37.0 37.0 37.0 25-29 36.5224 37.0 37.0 37.0 37.0 37.0 30-34 36.5044 37.0 37.0 37.0 37.0 37.0 35-39 36.4476 37.0 37.0 37.0 37.0 37.0 40-44 36.3634 37.0 37.0 37.0 37.0 37.0 45-49 36.3008 37.0 37.0 37.0 37.0 37.0 50-54 36.277 37.0 37.0 37.0 37.0 37.0 55-59 36.1931 37.0 37.0 37.0 37.0 37.0 60-64 36.0674 37.0 37.0 37.0 37.0 37.0 65-69 36.0569 37.0 37.0 37.0 37.0 37.0 70-74 36.0784 37.0 37.0 37.0 37.0 37.0 75-79 36.0937 37.0 37.0 37.0 37.0 37.0 80-84 36.0409 37.0 37.0 37.0 37.0 37.0 85-89 35.9675 37.0 37.0 37.0 37.0 37.0 90-94 35.9457 37.0 37.0 37.0 37.0 37.0 95-99 35.9306 37.0 37.0 37.0 37.0 37.0 100-104 35.8559 37.0 37.0 37.0 37.0 37.0 105-109 35.728300000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.566500000000005 37.0 37.0 37.0 37.0 37.0 115-119 35.619899999999994 37.0 37.0 37.0 37.0 37.0 120-124 35.586499999999994 37.0 37.0 37.0 37.0 37.0 125-129 35.4927 37.0 37.0 37.0 37.0 37.0 130-134 35.3374 37.0 37.0 37.0 34.6 37.0 135-139 35.2345 37.0 37.0 37.0 32.2 37.0 140-144 35.0636 37.0 37.0 37.0 27.4 37.0 145-149 35.0484 37.0 37.0 37.0 25.0 37.0 150-151 34.92425 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 3.0 22 1.0 23 1.0 24 8.0 25 6.0 26 10.0 27 9.0 28 13.0 29 23.0 30 44.0 31 39.0 32 56.0 33 115.0 34 173.0 35 442.0 36 2846.0 37 211.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.97392830283279 15.342191025319629 10.102782652293808 38.58109801955377 2 20.275000000000002 14.75 34.025 30.95 3 16.0 16.075 29.725 38.2 4 22.325 22.675 23.200000000000003 31.8 5 23.974999999999998 29.15 25.575 21.3 6 23.0 31.0 23.75 22.25 7 15.125 29.45 38.95 16.475 8 17.8 26.8 31.65 23.75 9 16.375 27.474999999999998 32.65 23.5 10-14 19.687953192978945 29.509426413962093 28.209231384707707 22.593389008351252 15-19 19.405 28.025 28.52 24.05 20-24 19.744999999999997 28.884999999999998 27.26 24.11 25-29 20.705000000000002 28.675 26.87 23.75 30-34 20.135 28.075 27.615000000000002 24.175 35-39 19.7 28.349999999999998 27.265 24.685000000000002 40-44 20.794999999999998 27.810000000000002 27.47 23.925 45-49 19.93 27.075 27.82 25.174999999999997 50-54 20.19 27.92 28.46 23.43 55-59 20.974999999999998 27.750000000000004 26.815 24.46 60-64 20.615 27.55 27.755000000000003 24.08 65-69 20.535 27.075 27.255000000000003 25.135 70-74 20.215 27.93 27.22 24.635 75-79 20.335 28.03 27.639999999999997 23.995 80-84 20.655 27.77 27.625 23.95 85-89 21.165 27.01 27.79 24.035 90-94 20.044999999999998 27.925 28.08 23.95 95-99 21.959999999999997 26.795 27.38 23.865 100-104 20.09 27.815 27.189999999999998 24.905 105-109 21.075 27.339999999999996 27.13 24.455 110-114 21.285 28.294999999999998 26.99 23.43 115-119 20.94 28.005000000000003 27.515 23.54 120-124 21.11 27.425 26.87 24.595 125-129 21.21 26.375 28.634999999999998 23.78 130-134 22.14 27.13 26.595000000000002 24.135 135-139 21.595 27.37 26.38 24.654999999999998 140-144 22.35 28.02 25.82 23.810000000000002 145-149 21.345 27.055 26.745 24.855 150-151 22.475 26.900000000000002 25.75 24.875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 1.0 24 1.5 25 6.0 26 12.5 27 14.0 28 11.0 29 17.5 30 23.0 31 22.5 32 36.5 33 43.0 34 61.5 35 82.0 36 82.5 37 86.5 38 100.0 39 120.5 40 162.0 41 199.5 42 214.0 43 222.0 44 251.5 45 267.5 46 263.0 47 243.5 48 227.5 49 198.5 50 164.0 51 164.5 52 138.5 53 106.5 54 92.5 55 82.5 56 65.0 57 47.0 58 31.5 59 25.0 60 26.0 61 18.5 62 14.5 63 13.5 64 5.0 65 4.0 66 2.5 67 4.0 68 4.5 69 3.0 70 3.5 71 2.5 72 3.0 73 3.0 74 0.5 75 1.0 76 1.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27499999999999997 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.015 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.324999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 62.03895565685868 37.425000000000004 2 22.420223787815996 27.05 3 9.034397016162453 16.35 4 3.5640281806879406 8.6 5 1.4090343970161625 4.25 6 0.9946125155408205 3.5999999999999996 7 0.20721094073767096 0.8750000000000001 8 0.16576875259013676 0.8 9 0.04144218814753419 0.22499999999999998 >10 0.12432656444260257 0.8250000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCCTAAGCAAATTACTTTCTTCAGGTGTACGCAACGATTTCCCTCCGCA 12 0.3 No Hit GTGCCATATATCCTATTGTTCCAGCAATTCGAGTACTGATGTGGGTTTTC 11 0.27499999999999997 No Hit CCCAACTGCTAACAATCGGTGCCAACAGAAGCAAAACAAATCCAAATGCT 10 0.25 No Hit GGGCAGAGAAAAAAAGAGAGAGGGGTTGAAAATTATATAAAGAAATTCTG 9 0.22499999999999998 No Hit CTCAACTTTAGCTTCTTCGGTTTTTGAATAATTCTCTAGCTGCTCAGCAT 8 0.2 No Hit CCTTGTTGGTCCACTTTAACACTGGGTCAATATCTCAGCCCCGTCTTTTG 8 0.2 No Hit AGTTGCTCATAAACCCCAGGGTTCATCAGTAAAAGGTCTCGACTTCCAGA 8 0.2 No Hit CACCACCTTCCCTGTACTTAAGAGGGAAAATGTGGCCTGGGCGGTTGAAA 8 0.2 No Hit GAATGAGCTCGCATTGAGAGGAACTGGCCCTCGATCACTGCTCGGACCTC 7 0.17500000000000002 No Hit GCTTGTGAAGGGAAAGTCCTGGAAAGGGTCCCAAATGTCAAGAGAGAAAG 7 0.17500000000000002 No Hit CTCATCCTCAAACCGTAGCATCTCAACAAAACCAACAGCTCCAGGTCCCT 7 0.17500000000000002 No Hit TGGCAAAAGAAAACGGGGGTGGGGAGAAAACCCAGTCCTGAAATATAGAG 7 0.17500000000000002 No Hit ACTTGAAGGTGTTAGTCACTGCAGCAGCTGCGCTAGACAAAATTCCACCA 7 0.17500000000000002 No Hit CGCCCCGTCCCTCTGAAGAAATGACATTCTCTTTAACTTCATTTTAAGTT 6 0.15 No Hit GTGGGATTCTTCTTGTTGACATTGTCTTGGCGAGCCTCATTAACCTCTTC 6 0.15 No Hit CATTGAAACAAGCTGTTGAGCTAGAGCTTCCAGCTTTCTCGACATAAGAT 6 0.15 No Hit CCTCTAGTTGTGCGATTCTTGCCTGAAGCTTGATTGTATCTAAGCTGTCA 6 0.15 No Hit CACCACTCGACTGTGGAACCGGACCAATAGACGCCAATTGACAAGCTGTC 6 0.15 No Hit ACCAGCTTCCATAAGAATTTGAACGTCCGTATCAGGAATCTGATGGCCAA 6 0.15 No Hit TCTCCGTCAAAAAGAACAAAAAAAATAAAATATAAAAACTAAATAGCTAA 6 0.15 No Hit GTTGTATACCCCGTAAGAACTGCATTTGTTCCTGCGGTGAACAACGTCTC 6 0.15 No Hit TGGCAGAAAAGCAGACATAAACTAGTCTAAACTGTAACTAAGCAAACACT 6 0.15 No Hit GGGAACATGTAAATTAAAAAAGAAAAAAGGAAAAATGAAAAGCAAGGAAT 6 0.15 No Hit GTGAAAACTACAAGATTTGAGAAGAAGAGAATGACTTGTAAGAACACACT 6 0.15 No Hit CCTGACGTATGCCTTCTTAGTTCCATCAGGCCTGATCAAAGTATTCACTT 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGATAGTATCTCGGTT 6 0.15 TruSeq Adapter, Index 6 (97% over 37bp) GGTAGGAACTTGTTAACAAGGCCAACTTCATAAGTAAAATGCACTGCATC 6 0.15 No Hit TGTTAATTTAATAATCAGTAATATTCAAAAGCATCTACTGTTGGTGGAGC 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGATAGTATCTCGTTT 6 0.15 TruSeq Adapter, Index 6 (97% over 37bp) GCTATGCGCAGGTCCAGTATCCGTTCATACACTGCTCGAGTGGACTCCAA 6 0.15 No Hit ATAGCTGATTTCGTCCAGATCACAAACCTTCCAAGATGACCACCAGGAGC 6 0.15 No Hit GCCTGGGAATGTGAGACCACAACTAGAATGCGGTCAAACTTCTTCAAAGT 6 0.15 No Hit GGCCAGATCTCTGCTTTCTTACCCGTCCGATGCGCTATCCGGGCCACCAC 6 0.15 No Hit TGAAATGGTCCAATTTAAATTTCAATGATCTTCTTCTTAGCTTCGTTTGT 6 0.15 No Hit ACGGAATTCTTGGTTACAAATAGATAGATGCTAGCCAGCCAAGATAAACA 6 0.15 No Hit GACAGAAAATAAAAACAGTCAAAAGAGTTGCTGCAAATTATTCCATAAAT 6 0.15 No Hit ACTACCCTCTCTTTCTCTCCGCTCCATTGCTTGCCTAGACTTGCTCCCTC 6 0.15 No Hit CCTGAGACAATCATCTAACCCAACTTTTCTGCTTCCCTTCATAGAAAGGC 5 0.125 No Hit AGCTTCTTAGACCAACCTGACAGGACAAGATTATTTAGAAGTAGGCTGTG 5 0.125 No Hit ATAGCAGATGCAGCAATAGCTGAAACGACAGCGTATCTCTTTTGGTTCAC 5 0.125 No Hit CGTCCAAGTGTTGACAGGATCCAAGATTTCTGAAGGCACGCCCTCAATCT 5 0.125 No Hit CCTGCCTCCATGTGATGATTAGTTGGCACTGAGCTTATATAGAACTCCTC 5 0.125 No Hit CACACAAGTCTGAATAGAAGATGTATTGAAATGGGCATTTTCTCTAGAGA 5 0.125 No Hit CACCTTTCTCTCCAGCTGACACAACAGAATCATCACCATTAGCAGCAGCA 5 0.125 No Hit GGATGATAAATGCCAAGACTATAGGAGAATATTTGTAAGCAAAGACATTT 5 0.125 No Hit AACCATCCTGCATTCCAGGCAGATGCACTGGCTGCCATTCATCAACACTT 5 0.125 No Hit CTCACGAACACCTAATAATTAAAAAAGTTAAGACTTTTCTGCCTACTCCC 5 0.125 No Hit ACCAGAGACATCACCGGTTAAATCTTTATCTTCAAAAGACATCTTTGCTA 5 0.125 No Hit GGTCAACTGAAATCAGGTTAGTTAGATTTTGTTCTCAAATGATAGTCACC 5 0.125 No Hit GGGATCAAAATAACAAAAACAAAGGAAATGATCCTGAAAACATGAGATGT 5 0.125 No Hit GTCCAATATCCATGCTTTTACATATCTCGTAAAATTCTGAAAGTCTCTCT 5 0.125 No Hit GTTTGCTCTTCAGCTTGAAAACTTCATCATAGTGAGCCATTATACCATCA 5 0.125 No Hit CTTGATAAGAAAATCGGCCTTAAACGAGGTTCAGAAGCATTACTATAACT 5 0.125 No Hit CCCTGTTCTTGATGACTCCAACACGGCCTCTGTTCCTACCTCCGGTGACC 5 0.125 No Hit ACCGAGAGGCCGAGATTGAACTATTGACTGAGTGGATGCTGGAATTGACA 5 0.125 No Hit AGTTGTTCTGCGCCCATTTGAGCCTGTCTTGACTTGTTGAATCAAGCTGT 5 0.125 No Hit AGTTGATGAAGTCAACGATTATGGACCGCTGGATTGGTGTAAACTGGCCA 5 0.125 No Hit CCTAGACTACTTTTAACTCTTTAAGCCACGATGTGTGTAGGGGGCAAGGA 5 0.125 No Hit GCTTGTGGTTGCGGGTTTTTTGGCTAGAGAAAGCAGAGTTCTGGAACAGT 5 0.125 No Hit CCACAATGAAACACCATCGTCCCAGTTGATATGGTCATCATCTCCATTAA 5 0.125 No Hit TCCAGAGATTGAAGTCTGAAAGAAGCATTTCCAATGCTTCTCCATTGTCC 5 0.125 No Hit GCCAAAACATTGAATGAGCTCCATGATTTTGTCACATGGCTTCCAGAATC 5 0.125 No Hit GGACTTTCTCTTCAAAACTACTACCCAAGTGATTGCTTCCAAGAGAGCAG 5 0.125 No Hit CAAGCATTAATAAATGATAACCCAAAGCCCAACTGAGGGGCCGCATATTC 5 0.125 No Hit GGTAGAGGAAGGAGTCTTCGGTGGCAGAGGTGAGATGTTTCCGGAGGAGG 5 0.125 No Hit CCCGCGACATCCATGCATCCATGTGCTGTTTTGCCACTCTCATGTACACG 5 0.125 No Hit CATCAACACCTTCATCCTCATCGCCTCCTTCAGCAGAAGGATTAGCGCCA 5 0.125 No Hit CCTCTCTCCGATAGTCAAATGAAGAATTCCGCGAGCCCAGTTTTTTCTTC 5 0.125 No Hit GTTATTATATGAGACTAGAAGCTCACTCTGGTCTGAGAATGACAAGCCTG 5 0.125 No Hit GTCAAAAACCCTGAATGCCTCTTTGAGCTCCTCCTCGGAGTCGGTATCCT 5 0.125 No Hit GCATGCAATTTCCAACTCCTATTCTTCACCACCAGCTGCCTAGAAGAGGG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.1125 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.3375 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.5 0.0 0.0 0.0 0.0 90-91 0.5874999999999999 0.0 0.0 0.0 0.0 92-93 0.625 0.0 0.0 0.0 0.0 94-95 0.75 0.0 0.0 0.0 0.0 96-97 0.9750000000000001 0.0 0.0 0.0 0.0 98-99 1.225 0.0 0.0 0.0 0.0 100-101 1.3 0.0 0.0 0.0 0.0 102-103 1.45 0.0 0.0 0.0 0.0 104-105 1.65 0.0 0.0 0.0 0.0 106-107 1.925 0.0 0.0 0.0 0.0 108-109 2.3499999999999996 0.0 0.0 0.0 0.0 110-111 2.6125 0.0 0.0 0.0 0.0 112-113 2.9125 0.0 0.0 0.0 0.0 114-115 3.25 0.0 0.0 0.0 0.0 116-117 3.6875 0.0 0.0 0.0 0.0 118-119 4.137499999999999 0.0 0.0 0.0 0.0 120-121 4.8125 0.0 0.0 0.0 0.0 122-123 5.4 0.0 0.0 0.0 0.0 124-125 5.762499999999999 0.0 0.0 0.0 0.0 126-127 6.237500000000001 0.0 0.0 0.0 0.0 128-129 6.762499999999999 0.0 0.0 0.0 0.0 130-131 7.3125 0.0 0.0 0.0 0.0 132-133 8.075 0.0 0.0 0.0 0.0 134-135 8.725 0.0 0.0 0.0 0.0 136-137 9.4875 0.0 0.0 0.0 0.0 138-139 9.825 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR26075321 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075321_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.348 37.0 37.0 37.0 37.0 37.0 2 36.3765 37.0 37.0 37.0 37.0 37.0 3 36.3175 37.0 37.0 37.0 37.0 37.0 4 36.3725 37.0 37.0 37.0 37.0 37.0 5 36.4225 37.0 37.0 37.0 37.0 37.0 6 36.457 37.0 37.0 37.0 37.0 37.0 7 36.4155 37.0 37.0 37.0 37.0 37.0 8 36.411 37.0 37.0 37.0 37.0 37.0 9 36.477 37.0 37.0 37.0 37.0 37.0 10-14 36.3703 37.0 37.0 37.0 37.0 37.0 15-19 36.335 37.0 37.0 37.0 37.0 37.0 20-24 36.2752 37.0 37.0 37.0 37.0 37.0 25-29 36.168899999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.1058 37.0 37.0 37.0 37.0 37.0 35-39 36.00169999999999 37.0 37.0 37.0 37.0 37.0 40-44 35.9784 37.0 37.0 37.0 37.0 37.0 45-49 35.9569 37.0 37.0 37.0 37.0 37.0 50-54 35.8257 37.0 37.0 37.0 37.0 37.0 55-59 35.8236 37.0 37.0 37.0 37.0 37.0 60-64 35.860800000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.7453 37.0 37.0 37.0 37.0 37.0 70-74 35.7149 37.0 37.0 37.0 37.0 37.0 75-79 35.6902 37.0 37.0 37.0 37.0 37.0 80-84 35.6811 37.0 37.0 37.0 37.0 37.0 85-89 35.62480000000001 37.0 37.0 37.0 37.0 37.0 90-94 35.547399999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.632099999999994 37.0 37.0 37.0 37.0 37.0 100-104 35.5459 37.0 37.0 37.0 37.0 37.0 105-109 35.4271 37.0 37.0 37.0 37.0 37.0 110-114 35.405899999999995 37.0 37.0 37.0 37.0 37.0 115-119 35.379000000000005 37.0 37.0 37.0 37.0 37.0 120-124 35.249 37.0 37.0 37.0 34.6 37.0 125-129 35.2635 37.0 37.0 37.0 37.0 37.0 130-134 35.23800000000001 37.0 37.0 37.0 32.2 37.0 135-139 35.10565 37.0 37.0 37.0 27.4 37.0 140-144 35.02815 37.0 37.0 37.0 27.4 37.0 145-149 34.9856 37.0 37.0 37.0 25.0 37.0 150-151 34.752375 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 3.0 14 1.0 15 3.0 16 5.0 17 7.0 18 3.0 19 4.0 20 7.0 21 5.0 22 13.0 23 4.0 24 8.0 25 10.0 26 11.0 27 17.0 28 11.0 29 18.0 30 20.0 31 46.0 32 60.0 33 84.0 34 172.0 35 700.0 36 2571.0 37 217.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.725 21.675 13.4 26.200000000000003 2 28.499999999999996 26.974999999999998 28.349999999999998 16.175 3 21.2 29.675 30.55 18.575 4 25.7 32.725 22.625 18.95 5 26.224999999999998 35.5 21.4 16.875 6 22.25 38.975 21.725 17.05 7 22.6 22.725 35.575 19.1 8 23.175 25.5 26.900000000000002 24.425 9 25.35 24.474999999999998 28.275 21.9 10-14 24.85 30.29 24.88 19.98 15-19 24.755 28.335 26.200000000000003 20.71 20-24 24.59 28.144999999999996 27.1 20.165 25-29 25.025 28.225 26.22 20.53 30-34 24.79 29.15 26.915 19.145 35-39 24.495 28.415000000000003 26.279999999999998 20.810000000000002 40-44 25.495 28.215 25.729999999999997 20.560000000000002 45-49 24.67 28.275 26.179999999999996 20.875 50-54 25.505 27.794999999999998 26.305 20.395 55-59 25.72 27.33 26.58 20.369999999999997 60-64 25.52 27.63 26.31 20.54 65-69 24.115000000000002 28.315 26.840000000000003 20.73 70-74 24.495 28.244999999999997 26.96 20.3 75-79 24.349999999999998 28.89 25.979999999999997 20.78 80-84 24.62 28.194999999999997 26.395000000000003 20.79 85-89 25.900000000000002 27.145000000000003 26.584999999999997 20.369999999999997 90-94 25.480000000000004 27.49 26.090000000000003 20.94 95-99 25.374999999999996 28.345 26.834999999999997 19.445 100-104 25.14 27.735 26.545 20.580000000000002 105-109 25.045 27.33 26.619999999999997 21.005 110-114 25.629999999999995 28.050000000000004 26.405 19.915 115-119 26.165 28.48 25.665 19.689999999999998 120-124 26.419999999999998 28.000000000000004 25.669999999999998 19.91 125-129 25.924999999999997 27.785 27.029999999999998 19.259999999999998 130-134 25.61 28.365000000000002 26.58 19.445 135-139 28.0314015700785 27.58137906895345 25.721286064303218 18.665933296664832 140-144 27.25681420355089 28.722180545136283 25.386346586646663 18.63465866466617 145-149 27.15129077446468 27.9217530518311 26.33580148088853 18.59115469281569 150-151 27.68538201825685 28.76078529448543 25.109416031011627 18.444416656246094 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.5 12 1.0 13 1.5 14 1.0 15 0.0 16 1.5 17 1.5 18 0.5 19 0.5 20 0.0 21 0.5 22 1.0 23 0.5 24 1.5 25 4.0 26 6.0 27 5.5 28 5.0 29 12.5 30 14.5 31 20.5 32 30.5 33 25.0 34 28.0 35 44.0 36 66.0 37 89.5 38 109.0 39 147.0 40 163.0 41 210.5 42 264.0 43 252.0 44 274.0 45 296.0 46 260.0 47 234.5 48 225.0 49 188.0 50 168.5 51 164.0 52 126.5 53 100.0 54 92.0 55 75.5 56 62.5 57 47.5 58 28.0 59 20.0 60 15.5 61 9.0 62 10.5 63 9.5 64 12.0 65 8.0 66 1.5 67 2.5 68 2.5 69 1.5 70 1.0 71 2.5 72 4.5 73 2.5 74 0.0 75 0.5 76 1.0 77 2.0 78 2.0 79 1.0 80 1.0 81 1.5 82 2.0 83 2.5 84 2.5 85 1.5 86 1.0 87 1.5 88 1.5 89 1.0 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 10.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.005 140-144 0.025 145-149 0.06 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.324999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 63.67713004484305 39.050000000000004 2 22.054626987362415 27.05 3 8.23481451284142 15.15 4 3.220546269873624 7.9 5 1.3045250713412149 4.0 6 0.8560945780676722 3.15 7 0.24459845087647777 1.05 8 0.16306563391765186 0.8 9 0.08153281695882593 0.44999999999999996 >10 0.16306563391765186 1.4000000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 24 0.6 No Hit GTACGATCCGCTTAGCTACGCTCTCAATTTCGATGAAAACGTGAGTCGAG 11 0.27499999999999997 No Hit AATTGTTCACAGGGACATCAAACCTACCAATGTTCTGCTTGATAGGGATC 11 0.27499999999999997 No Hit CTTCACTTGGATTATGTCAGTGTGAAAAGGATGAGTGGAAAGCTATTCAG 10 0.25 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 9 0.22499999999999998 No Hit CAGAAAGGGAAAGAAAAATAAGAAAGGAAGACGAAGAATCCTCTCGGAGA 9 0.22499999999999998 No Hit AGATGATTGGGGTTGGAGGGAGTGATTGGAAGCATTATGTTCGAAGAAAA 8 0.2 No Hit AGAAAATTGGCAAAACAATACAATAGATATTCTTACGGTGTTGTCCGACA 8 0.2 No Hit CAAAAAGCCTGATGCAAAGGCACAAGCCCAGAAGGCTGCCAGGGCTGTGA 8 0.2 No Hit CGGTGACAGTGGATGCGAAAAATGGGACAACTACAGGAGTATCAGCTCGT 8 0.2 No Hit CATGAAGACACAGGGGACTGAAAGGATAAACGAAGAAGGTGAAGCTGAAA 7 0.17500000000000002 No Hit CTGGAATCGGTTATAAAAAGTTTTGAAGAGGAGATTCACGTAGGGGTTCC 7 0.17500000000000002 No Hit CAAACACAAGACTTTTGCGCCGCTGTGATATCAAATGTGGGATAAGATGC 7 0.17500000000000002 No Hit GGTTTCTACTACAAGGACCATGAAATTCTCCCTCCCCTTCCAGTTGGCTT 7 0.17500000000000002 No Hit GTTTTCCCTTTCTTCTCAAATACCTCAACAAGAAACCAAGAAAATGTCTC 7 0.17500000000000002 No Hit GAGGGAATCTTATTGGCCATAGCAACTGCCGGTTTATCTCCTCAGAGATC 7 0.17500000000000002 No Hit AGCGGAAAAAAATCCGCCAGAAGAATGAGAAAAAGCATCAGCGTCAAAAG 6 0.15 No Hit GGATTACTACTAGCGGGGTCCAAAGCGAGTCTTCATCATCGGCCGTTGAT 6 0.15 No Hit TGAGAATGATCCCACGACATTTCCAAAGGCGATACACTATACTCGTGGAG 6 0.15 No Hit TTTAAGCCTTATGGAGAGATTGAGGATTGTAAAGCTGTGTGCGATAAGGT 6 0.15 No Hit CTGCCAAGGAGGCTGCCAACTTCACCTCTCAGGTCATCATCATGAACCAC 6 0.15 No Hit GTTACATTTCACCGTCCCAGGACATTGAAGAAGGAGAGGAATCCCAAGTA 6 0.15 No Hit ATCGTTAAGGAGGAGGATGTGGATTTGTACAGGAAGCTCGTCGTCAGCTC 6 0.15 No Hit TATATTTCGAGGTTATCAAAGATGCCTAGCATTATCCATGGTGCCCCCCA 6 0.15 No Hit GTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGA 6 0.15 No Hit GGATTACAATGGATTCACCGAATCTTTCCCACTTAGCTTACTTATTCTAC 6 0.15 No Hit CGGAGATTTCCAGGGCACTGGCTGAGTCGAAATATAATATGGAGATCCAA 6 0.15 No Hit GCTAAGGATTCTCACGCGATCCGGCCTGGAAAGGGGAAAATGAGGAACAG 6 0.15 No Hit TGAAGATGGAATCAAGAGCTGATTTGATGAAGAGAAAGGCTATGAAAATT 6 0.15 No Hit GATTTCTGGGCAGCAGAGATTTTGACAGGCAAAGGACGGATAGGTGGTGA 6 0.15 No Hit CAGGTACAAGAAACGCAAGCAATTGCAGACGGTGGAGGTGAAAGTCCGGA 6 0.15 No Hit GGAAGTCGCTGGCATAAAGGAGGGATTCAAGATTATGGATACTGGAAATA 6 0.15 No Hit TGGTGGGTCAATAATTTCTGATGTTACACTGAGAAACTGATTTGTGACCT 6 0.15 No Hit GAAACCCTGCTGGAGCTCCGTCGTCTCCTCCTGGAAACGGCGCTGCTTTG 6 0.15 No Hit GGAAAAAGGAAAGAAAAAAATCTCCTTCGTTTCTTCTTCTATTGAAGCTT 6 0.15 No Hit TTTATGGGGGGAAAAAGAAGAAATTCCTCAATTCATGCTGACTTGTGGAA 6 0.15 No Hit GGTAGCAGTTATGAAGAAAATTTCGAGGGAAGCAAGGACTCGGGGGACAC 6 0.15 No Hit CATTTTTCAAAAGAGAAATGGCGGATCAATTGACGGATGACCAGATCTCT 5 0.125 No Hit CAAAGTGCTACAGACATTTTATCCTGAGAAAGACTTAACTGCTGCTCCAT 5 0.125 No Hit GGTGCTTTGGGAAAGCCCAATACTGGACGCAGTTCTTTCCAGATGTATCC 5 0.125 No Hit AGAAAGAATTCCGCTATGAGGTGTCTAGCCTTATTACAGAACTTGCTTCT 5 0.125 No Hit GCCATGTGAAGAAAAGAAAGAGATAGCAAATGGTTCATAGAAAGTCTCTG 5 0.125 No Hit GTTCTTTGAGATGACGCGCCTTGATGCTTTGAAGGCTCTGGAAATATATA 5 0.125 No Hit TTTCAAAGCAGCCAGAGTCTGGCTCGGAACCTTCGACACCGCCGAGGCCG 5 0.125 No Hit GTGTGATTGTATAGCTGGTCTGGAGTGCCTTTTAAACATTATCAGGGCCC 5 0.125 No Hit GAGGAAGTGAAAGTCGAGATTGAAGATGACAGGGTGCTTCAAATTAGCGG 5 0.125 No Hit CCAAGTTCTTGACGTTTGAGCCTGAGTTGACTTCTCAGGTTTGCTTTGTA 5 0.125 No Hit TTCAACAATACATGGGGCAGTTGCAGGCATGGCTCTCCAGCCAAAGTCCA 5 0.125 No Hit GTTGGGATGGGTCAAGTGATTTTGGTCAACCTGCAGATTACTTCTGTCCT 5 0.125 No Hit CTTAGAGAGCAACAAGATTACTGACTTCATCAAGTTTGATGTTGGCAATG 5 0.125 No Hit CTTCATATCAAGCTCAGGAGACCAAACTCATTCAATGAGTGGAAATGGGG 5 0.125 No Hit GTTAAAGAGAGACTCGATGAAGGGAAAGCTGGACTGATAGCCGTGTCAAA 5 0.125 No Hit CAGCGAGCTTCGGTAGGGGTGGGTCAAGGACCAGTGCTATGACAGGGAAA 5 0.125 No Hit CTCTCAACGAAAGTCGAATCGAAGTCCTTTCAAAACTCTCTCAAGATGCA 5 0.125 No Hit CTTTTGGATGCTACTATGAATATGGAAGGAGTTTTGCTGGCTGGAGTTCC 5 0.125 No Hit GAAGAGCAGATGCAACCTTTACCTCCACCTGTTCAACTTGGTGAAGTCAC 5 0.125 No Hit CCAAGAGACGAAGCAAAGCCTTCGATGCAGACAGCTTAAGTCCTTTGGCT 5 0.125 No Hit CCCGGGTTGGAAGGAGCAGAAAGAGGTCTAAGAAGAAGAACCGAAAACAT 5 0.125 No Hit GTCTCGATGAAGTCTTTGATTTTTCCCCTTTTTTCTTCTTCTTCCCACCA 5 0.125 No Hit CAACTACGTCCACTCAAACATCTCCAAGAATAGCCGCCAGCCTTATGCCG 5 0.125 No Hit AAACAACCATGTTGCCAAGCCTTCTAAATTTATTCAAGTTCGCTATGAAG 5 0.125 No Hit TCTCGGACTCGTTCCCCTACAAGGAAATTGAGAATGGGATACTGTGGGAA 5 0.125 No Hit GAATTTCTTTTTAAACTTCAAAATCACATTTCTTGCCGAGAATACATGGC 5 0.125 No Hit AAGGTTTTCTGTCACCTGTTGGACCCCGTGGAAGGCTCTTCAGGAAGTTA 5 0.125 No Hit CCCCAAGACTGAAGAAGAGAAGGAGAAAGAAAAGGAGAGTGCTTCCCAGT 5 0.125 No Hit CAACGATCCACAGCGGAGTTCCTCTTCAATCTCTGCAAACAAACTCACCC 5 0.125 No Hit AGGACCACAAGATTCGATTTTACTGGAATATCTATCACCATGGAATTGGA 5 0.125 No Hit GTTACCGGCCGCGGCAGCACATGGGAAGGAAGTAGTGGAAGAGGCACTGG 5 0.125 No Hit TGGAAAGGGGAAGATAACGGTTAGTTGTATTGAAAATCAGCCTGCAGTGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.1125 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.3125 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.475 0.0 0.0 0.0 0.0 90-91 0.5625 0.0 0.0 0.0 0.0 92-93 0.6 0.0 0.0 0.0 0.0 94-95 0.7250000000000001 0.0 0.0 0.0 0.0 96-97 0.9625 0.0 0.0 0.0 0.0 98-99 1.225 0.0 0.0 0.0 0.0 100-101 1.3 0.0 0.0 0.0 0.0 102-103 1.45 0.0 0.0 0.0 0.0 104-105 1.675 0.0 0.0 0.0 0.0 106-107 1.95 0.0 0.0 0.0 0.0 108-109 2.3625 0.0 0.0 0.0 0.0 110-111 2.625 0.0 0.0 0.0 0.0 112-113 2.9375 0.0 0.0 0.0 0.0 114-115 3.2750000000000004 0.0 0.0 0.0 0.0 116-117 3.725 0.0 0.0 0.0 0.0 118-119 4.15 0.0 0.0 0.0 0.0 120-121 4.85 0.0 0.0 0.0 0.0 122-123 5.475 0.0 0.0 0.0 0.0 124-125 5.8375 0.0 0.0 0.0 0.0 126-127 6.3125 0.0 0.0 0.0 0.0 128-129 6.85 0.0 0.0 0.0 0.0 130-131 7.45 0.0 0.0 0.0 0.0 132-133 8.225 0.0 0.0 0.0 0.0 134-135 8.9 0.0 0.0 0.0 0.0 136-137 9.675 0.0 0.0 0.0 0.0 138-139 10.0125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561601 spots for SRR26075321.sra Written 1561601 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra Read 1561585 spots for SRR26075321.sra Written 1561585 spots for SRR26075321.sra SRR ids: ['SRR26075321.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_hpbjpj08 SRR26075321.sra spots: 31231716 blocks: [[1, 1561585], [1561586, 3123170], [3123171, 4684755], [4684756, 6246340], [6246341, 7807925], [7807926, 9369510], [9369511, 10931095], [10931096, 12492680], [12492681, 14054265], [14054266, 15615850], [15615851, 17177435], [17177436, 18739020], [18739021, 20300605], [20300606, 21862190], [21862191, 23423775], [23423776, 24985360], [24985361, 26546945], [26546946, 28108530], [28108531, 29670115], [29670116, 31231716]] SRR26075321 file size 11532244 SRR26075321 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075321 SRR26075321_1.fastq SRR26075321_2.fastq Input file: SRR26075321_1.fastq Paired file: SRR26075321_2.fastq trimmed: SRR26075321-trimmed-pair1.fastq, SRR26075321-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 18:37:57 2025 >> started Tue Feb 11 18:38:35 2025 >> done (38.207s) 31231716 read pairs processed; of these: 160 ( 0.00%) short read pairs filtered out after trimming by size control 152600 ( 0.49%) empty read pairs filtered out after trimming by size control 31078956 (99.51%) read pairs available; of these: 4122477 (13.26%) trimmed read pairs available after processing 26956479 (86.74%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 16 0.00% 19 13 0.00% 20 9 0.00% 21 20 0.00% 22 16 0.00% 23 26 0.00% 24 34 0.00% 25 29 0.00% 26 43 0.00% 27 47 0.00% 28 43 0.00% 29 49 0.00% 30 47 0.00% 31 67 0.00% 32 57 0.00% 33 66 0.00% 34 39 0.00% 35 64 0.00% 36 82 0.00% 37 40 0.00% 38 105 0.00% 39 79 0.00% 40 96 0.00% 41 108 0.00% 42 94 0.00% 43 84 0.00% 44 102 0.00% 45 89 0.00% 46 112 0.00% 47 120 0.00% 48 122 0.00% 49 162 0.00% 50 165 0.00% 51 225 0.00% 52 180 0.00% 53 206 0.00% 54 234 0.00% 55 287 0.00% 56 269 0.00% 57 341 0.00% 58 319 0.00% 59 416 0.00% 60 440 0.00% 61 522 0.00% 62 601 0.00% 63 597 0.00% 64 739 0.00% 65 808 0.00% 66 866 0.00% 67 1038 0.00% 68 1073 0.00% 69 1269 0.00% 70 1443 0.00% 71 1695 0.01% 72 2005 0.01% 73 2306 0.01% 74 2644 0.01% 75 3035 0.01% 76 3381 0.01% 77 3534 0.01% 78 3995 0.01% 79 4622 0.01% 80 4950 0.02% 81 5842 0.02% 82 6758 0.02% 83 7389 0.02% 84 8260 0.03% 85 9197 0.03% 86 9926 0.03% 87 11292 0.04% 88 11685 0.04% 89 12921 0.04% 90 14070 0.05% 91 15315 0.05% 92 16236 0.05% 93 17586 0.06% 94 19863 0.06% 95 21129 0.07% 96 22574 0.07% 97 25111 0.08% 98 26131 0.08% 99 27064 0.09% 100 29014 0.09% 101 29783 0.10% 102 31294 0.10% 103 32919 0.11% 104 34364 0.11% 105 36979 0.12% 106 39756 0.13% 107 40613 0.13% 108 42860 0.14% 109 44326 0.14% 110 45930 0.15% 111 47099 0.15% 112 49140 0.16% 113 49946 0.16% 114 51545 0.17% 115 55595 0.18% 116 56719 0.18% 117 58920 0.19% 118 60873 0.20% 119 63536 0.20% 120 63801 0.21% 121 65405 0.21% 122 66904 0.22% 123 68739 0.22% 124 71722 0.23% 125 72468 0.23% 126 76033 0.24% 127 78100 0.25% 128 80252 0.26% 129 82908 0.27% 130 85233 0.27% 131 85735 0.28% 132 86616 0.28% 133 89579 0.29% 134 90494 0.29% 135 92912 0.30% 136 95758 0.31% 137 96427 0.31% 138 100332 0.32% 139 103351 0.33% 140 103900 0.33% 141 106397 0.34% 142 105667 0.34% 143 106808 0.34% 144 111084 0.36% 145 111252 0.36% 146 113298 0.36% 147 114351 0.37% 148 117745 0.38% 149 121024 0.39% 150 122337 0.39% 151 26956479 86.74% 31078956 reads passed initial QC criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=2.93 fanout-score-rank=27 prefix-density=0.39 prefix-fanout=2.4 sequence=GCAAAGATCAACCTCTGCTGGTCTGG criterion=fanout-score sequence-density=0.03 sequence-density-rank=32 fanout-score=126.86 fanout-score-rank=1 prefix-density=0.33 prefix-fanout=10.9 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=2.54 fanout-score-rank=26 prefix-density=0.36 prefix-fanout=2.4 sequence=CCAGACCAGCAGAGGTTGATCTTTGC criterion=fanout-score sequence-density=0.13 sequence-density-rank=17 fanout-score=292.50 fanout-score-rank=1 prefix-density=1.13 prefix-fanout=33.7 sequence=AAGAAGAAGAAA SRR26075321 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 18:40:21 Started mapping on | Feb 11 18:40:21 Finished on | Feb 11 19:14:58 Mapping speed, Million of reads per hour | 53.87 Number of input reads | 31078956 Average input read length | 287 UNIQUE READS: Uniquely mapped reads number | 17124412 Uniquely mapped reads % | 55.10% Average mapped length | 287.98 Number of splices: Total | 16049930 Number of splices: Annotated (sjdb) | 15619017 Number of splices: GT/AG | 15727070 Number of splices: GC/AG | 251638 Number of splices: AT/AC | 20444 Number of splices: Non-canonical | 50778 Mismatch rate per base, % | 0.43% Deletion rate per base | 0.03% Deletion average length | 3.78 Insertion rate per base | 0.02% Insertion average length | 2.54 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 420033 % of reads mapped to multiple loci | 1.35% Number of reads mapped to too many loci | 59741 % of reads mapped to too many loci | 0.19% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 42.99% % of reads unmapped: other | 0.36% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 13534511 13534511 13534511 N_multimapping 420033 420033 420033 N_noFeature 487364 16938119 594806 N_ambiguous 269313 2869 188416 UnstrandedReadsAssigned:16367735 PositiveStrandReadsAssigned:183424 NegativeStrandReadsAssigned:16341190 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075321 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075321-trimmed-pair1.fastq SRR26075321-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 31,078,956 reads, 19,330,388 reads pseudoaligned [quant] estimated average fragment length: 213.327 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,222 rounds 52401 SRR26075321.ke.tsv 34699 SRR26075321.se.tsv 87100 total ==> SRR26075321.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1805.67 2352 63.7934 Potri.005G024800.1.v4.1 1035 822.673 6679 397.614 Potri.004G059700.1.v4.1 961 748.673 2 0.130832 Potri.007G009000.2.v4.1 1416 1203.67 0 0 Potri.003G141000.2.v4.1 2943 2730.67 1205.46 21.6203 Potri.016G087400.1.v4.1 270 91.9495 1863 992.296 Potri.015G069301.1.v4.1 564 353.901 0 0 Potri.010G195200.1.v4.1 1773 1560.67 398 12.4896 Potri.012G127500.1.v4.1 977 764.673 3992 255.677 ==> SRR26075321.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 68 Potri.001G233950.v4.1 2 Potri.001G122700.v4.1 260 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 399 SRR26075321 completed mapping pipeline successfully