Starting /dee2/code/volunteer_pipeline.sh SRR26075325 current disk space = 3053432848384 free memory = 1448577736 SRR26075325 SRAfilesize a28cd8e634f865d81bbe5096cfeb00b6 SRR26075325.sra SRR26075325.sra file validated SRR26075325 is paired end SRR26075325 is conventional basespace SRR26075325 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075325_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6295 37.0 37.0 37.0 37.0 37.0 2 36.514 37.0 37.0 37.0 37.0 37.0 3 36.5975 37.0 37.0 37.0 37.0 37.0 4 36.718 37.0 37.0 37.0 37.0 37.0 5 36.75 37.0 37.0 37.0 37.0 37.0 6 36.6135 37.0 37.0 37.0 37.0 37.0 7 36.646 37.0 37.0 37.0 37.0 37.0 8 36.612 37.0 37.0 37.0 37.0 37.0 9 36.6395 37.0 37.0 37.0 37.0 37.0 10-14 36.632549999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.58389999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.55550000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.522999999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.4307 37.0 37.0 37.0 37.0 37.0 35-39 36.468 37.0 37.0 37.0 37.0 37.0 40-44 36.4024 37.0 37.0 37.0 37.0 37.0 45-49 36.2558 37.0 37.0 37.0 37.0 37.0 50-54 36.1782 37.0 37.0 37.0 37.0 37.0 55-59 36.1584 37.0 37.0 37.0 37.0 37.0 60-64 36.1143 37.0 37.0 37.0 37.0 37.0 65-69 35.9525 37.0 37.0 37.0 37.0 37.0 70-74 36.0479 37.0 37.0 37.0 37.0 37.0 75-79 36.035999999999994 37.0 37.0 37.0 37.0 37.0 80-84 36.0341 37.0 37.0 37.0 37.0 37.0 85-89 35.923100000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.9062 37.0 37.0 37.0 37.0 37.0 95-99 35.8408 37.0 37.0 37.0 37.0 37.0 100-104 35.8593 37.0 37.0 37.0 37.0 37.0 105-109 35.729 37.0 37.0 37.0 37.0 37.0 110-114 35.5947 37.0 37.0 37.0 37.0 37.0 115-119 35.4952 37.0 37.0 37.0 34.6 37.0 120-124 35.5589 37.0 37.0 37.0 37.0 37.0 125-129 35.25500000000001 37.0 37.0 37.0 34.6 37.0 130-134 35.2774 37.0 37.0 37.0 32.2 37.0 135-139 34.990500000000004 37.0 37.0 37.0 25.0 37.0 140-144 34.83650000000001 37.0 37.0 37.0 25.0 37.0 145-149 34.7356 37.0 37.0 37.0 25.0 37.0 150-151 34.667249999999996 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 0.0 22 2.0 23 3.0 24 2.0 25 8.0 26 16.0 27 11.0 28 22.0 29 26.0 30 31.0 31 46.0 32 83.0 33 103.0 34 164.0 35 484.0 36 2841.0 37 157.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.46670005007511 16.599899849774662 4.957436154231347 33.97596394591888 2 19.875 14.499999999999998 34.300000000000004 31.324999999999996 3 17.175 17.599999999999998 30.825000000000003 34.4 4 22.425 22.55 26.375 28.65 5 25.025 29.525000000000002 24.625 20.825 6 23.275000000000002 33.074999999999996 22.175 21.475 7 15.35 30.95 37.95 15.75 8 18.925 28.1 30.525000000000002 22.45 9 19.8 24.349999999999998 34.425 21.425 10-14 20.32601630081504 29.396469823491174 27.87139356967848 22.4061203060153 15-19 20.14 27.505000000000003 28.83 23.525 20-24 20.815 27.63 28.535 23.02 25-29 20.805 26.995 27.955000000000002 24.245 30-34 18.83 28.410000000000004 28.42 24.34 35-39 20.845 27.36 27.865000000000002 23.93 40-44 20.275000000000002 26.625 27.865000000000002 25.235000000000003 45-49 19.77 27.97 27.99 24.27 50-54 20.95 27.284999999999997 27.315 24.45 55-59 20.01 27.860000000000003 28.26 23.87 60-64 21.060000000000002 27.800000000000004 26.99 24.15 65-69 20.669999999999998 27.93 27.884999999999998 23.515 70-74 20.735 27.325 28.24 23.7 75-79 21.42 28.275 25.895000000000003 24.41 80-84 21.33 27.605 27.74 23.325000000000003 85-89 21.775 27.305 27.939999999999998 22.98 90-94 20.285 27.235 28.720000000000002 23.76 95-99 20.990000000000002 27.365000000000002 27.36 24.285 100-104 21.59 27.584999999999997 27.255000000000003 23.57 105-109 21.075 28.32 27.715 22.89 110-114 20.69 28.305000000000003 27.605 23.400000000000002 115-119 20.875 27.58 27.51 24.035 120-124 21.41 27.55 27.05 23.990000000000002 125-129 20.57 28.535 26.745 24.15 130-134 21.19 27.884999999999998 26.735 24.19 135-139 21.68 28.000000000000004 26.105 24.215 140-144 22.45 27.705000000000002 25.775 24.07 145-149 22.23 27.944999999999997 25.330000000000002 24.495 150-151 22.900000000000002 26.900000000000002 25.8125 24.3875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 1.0 20 3.0 21 3.5 22 1.0 23 2.0 24 4.0 25 2.0 26 1.5 27 9.5 28 19.0 29 16.0 30 10.0 31 16.0 32 28.5 33 35.0 34 42.5 35 44.0 36 48.5 37 80.0 38 116.5 39 165.5 40 176.5 41 191.5 42 249.0 43 280.5 44 268.0 45 254.5 46 269.0 47 281.5 48 254.0 49 212.0 50 178.5 51 144.5 52 133.5 53 98.0 54 66.0 55 61.5 56 52.0 57 31.5 58 21.0 59 19.0 60 16.0 61 20.5 62 13.5 63 7.5 64 6.5 65 4.5 66 3.5 67 3.5 68 6.0 69 3.5 70 3.0 71 4.5 72 5.5 73 5.0 74 2.0 75 0.5 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.550000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 64.54102355808286 39.725 2 21.283509341998375 26.200000000000003 3 7.27051177904143 13.425 4 3.777416734362307 9.3 5 1.6653127538586516 5.125 6 0.6904955320877335 2.55 7 0.4467912266450041 1.925 8 0.16246953696181965 0.8 9 0.08123476848090982 0.44999999999999996 >10 0.08123476848090982 0.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTGAAAAGAATGGCATACAACTGAAACACCAAGCGGCAATCACAGCACA 10 0.25 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCACCATCTCGGTT 10 0.25 TruSeq Adapter, Index 3 (97% over 36bp) GGTCATCACAGCAGGTGGCACCCTCGAGTGGGCAGCATCCCCATGCAAAG 9 0.22499999999999998 No Hit TCTCTGTCTCCTTCATGCTCCCTATGAAGCTCCCGGTGATTGACTTTCTC 9 0.22499999999999998 No Hit GGAAACATGTCTGGGAAGAATTACCATTTGATGAGGTCAAGCCTATCAAC 8 0.2 No Hit AGTTACAGGCTCAAACTTTGAAATTGCAATTGCCACTTTGGTGAAAACAA 8 0.2 No Hit CAAAATTTTTTGAAGTGCAGGCACTTCCATTTCTTGAACAGTCTTTGTAG 8 0.2 No Hit GCAAGAGGAGGAGCCAGAGGTCCAAGTGCATGCTTGAGCATTAAAGTTCC 8 0.2 No Hit CATGGAAGGAATTGGTATGGGTTGCTCTGCTAAAACCAGAGAGTCAAGGT 7 0.17500000000000002 No Hit GCTTCATCAAAATCCAAGGTTCCATTGCCATCCTTGTCCAGCGCTGTAAA 7 0.17500000000000002 No Hit GGGTTAATACCAAACAATGTTTCCAAGGTTAAAATAAAAGGCAAAATGAT 7 0.17500000000000002 No Hit GCCGGAAAGAGTCCTCTTCAAGTGGCGCACAGATTTTCCTTGTCATAACT 7 0.17500000000000002 No Hit GTCTCTCCTCCTGGCATTCTCTCCTGGATGGACAAACGGGCACTCAGTCC 7 0.17500000000000002 No Hit CTCGTGAACAAGTATGTCACAGCCATTCCCAAGAGACAAATCCCACCCAA 7 0.17500000000000002 No Hit CGTTGCTACTTTCCTCCTAGTTGCTCTGTTCCGAATCAAAAACAGAAACT 7 0.17500000000000002 No Hit GTTATGTATTCAGAAGAGTGGGTGACTTGGTGTTTGGCGTAGAATTCAGT 7 0.17500000000000002 No Hit GGCAGCCTCAGGAGAGTAAGCATATGGAGATGGTGGACTCCTGCTGCCAT 7 0.17500000000000002 No Hit GTGGAGATGGGCATGATTGGGAATATTATCAGAAAGTGGAGGGTAATTAA 7 0.17500000000000002 No Hit GCCTACTCTCAAGAAAAACCTTGCTGAATGATGGGATTTCCTCTATCAAT 7 0.17500000000000002 No Hit CATAAAAGATCCATCTCCATCATCCACCTCTCCATCCGGATTTTCATAAT 6 0.15 No Hit ACAGGTCTCAAGCAGTGCTAGCACTTGATTGGAGCCTGATAAGGCCTCTA 6 0.15 No Hit CGTCAAGGGTGGTAGGTTTCAGATCTGCCATCCTACTGCTCCAGCGAGAG 6 0.15 No Hit GCAGAGGCATTATTTTGGTTCAATATCGATGTCTGAGCAAAGTTTGAATT 6 0.15 No Hit GGTGGTTGTGGTTGTGGTTGTTTTAGGGCTATTGGTGTTGGTGTCAATGA 6 0.15 No Hit GTGGTGCTGTCAGCTGTAAAACTGTTTGGCGAATCTCCCTGAGAGCACTC 6 0.15 No Hit CATCTTTTGATACCCTTGGCATTGGTACTTCTTGCTGTTCCATCCCATCC 6 0.15 No Hit CTTTCAATGCCTTTCGGAACAGACCAGGTGGGATGTTGGAAATTCTAAGT 6 0.15 No Hit GCCGACGTATCAAGCGTGGGCAGGTACCCGCTACCCACTAGCAACCGGCC 6 0.15 No Hit ATCCGGAGTATGGGCATTCAGTTAAAACCAAAGATGCTGTTTTGCAGCAA 6 0.15 No Hit ATCAAATCTCTATCATTCAACAATATCCACATACTCATCACCTTCCTCCA 6 0.15 No Hit GCTTCAAGAGCTTCACTGGAGACGTTCTCCTTCTCCCAGGACACCAAGTG 6 0.15 No Hit CAGAGATGAATACCAAAACCCTATGACATAGTTCTTTCAAATGGTAATTC 6 0.15 No Hit CATCACCAGAGAGAAGATCTTGATAGACCAACATGATTGCGCAGAAGAGC 6 0.15 No Hit CCTAAATAAAAATTCCATACATAATCATCATCTAAACAGCATTATCGTCA 6 0.15 No Hit CCGCCTAAAAGGGACTGTAGATTCCCTTCTCTCAACTCTTTCCTCAATAA 6 0.15 No Hit GTCAACTTCTGCAGGAATTCAGGAGGTGCAGATTGTCCAACAAGTGAAAC 6 0.15 No Hit CCTTAACGTTTTTGGCATTAATCTGGTTTGCAAATCCTTTGTTGCCGACA 5 0.125 No Hit CATCTTCTGACTAGATCTGTCAAATATTCATATCCCAAGTCCTCTGCGGT 5 0.125 No Hit GGACTGCGTCAAGCGGGAGCCAATGGTACGTCGCATATTCGAAATGGGAG 5 0.125 No Hit GGGGCATGTTAGAGGTGTAGAACAGCTTGATTGGATATGAACCCTGCTGC 5 0.125 No Hit ATGTAGCTTTTGGAATCTTTACACTCTCCACAGGTCCATCGTCCTTGTGC 5 0.125 No Hit GTGTTAATAATTACTCCTGCATTCGGATAACAATATAATATTGCCGATTG 5 0.125 No Hit CCAGGCTATATCTGATTCATAATAGTCATTTGAGAGGAATGCGTCAGCCT 5 0.125 No Hit ACTGGATCGACATGGCTTTCTTGTTTGCTTCAAGCTGACTTCCCTTGGAA 5 0.125 No Hit CCCGTAAGAATCTCAGTGCATAAATAAGTAGTGCCAAATTGTCCTTGTCC 5 0.125 No Hit GGGTAAGGCTAACTTTGTTCTGCCACAAGCCTCCACTCTTGGGAGATGAG 5 0.125 No Hit GTCCAACGCTTTCCATGGTGATACCAATTCAGGTGTTCTGGCTCTTCCAG 5 0.125 No Hit CGAGTGGACGGCAGCGATTGAGCCTCGTAGATACTCAGAATCAAGAGTCA 5 0.125 No Hit GTTAGTTGTTAAAGGAGGCTTAAGGAATTGAGAGCATTTAGAGATGGGAG 5 0.125 No Hit TATATGGCCTGAATGGGGATCATAAAACCTTTGCAATAACGAGATCACCG 5 0.125 No Hit ACTGCTCACTATTTGGCATTGGTGTGCCATTGTATGTCTGCAAAATTCTC 5 0.125 No Hit GCTGGATCGATGGTCTTCATGGCTTCCAGCGGATAATAATACCTCCAAAG 5 0.125 No Hit GTCAGGAAGGATGTAATAGCGAATGTTGTTGCCCCTCACGCTGAGATGAT 5 0.125 No Hit AGGGGTAGGGAGGGCAGGGATTGCGCCCTTTTTGGTGGTTGTGATGGCTC 5 0.125 No Hit ACCTGGACCACAAATTGGATTATTTGGTCAAGCAAAGCATAAAGAGCCAT 5 0.125 No Hit TGGATTTGGATTTTCTTGAAAGGTTGAGACGCAGATCCGGCCATTGCACG 5 0.125 No Hit GTGATAGGTGGCTGAGACGATTAAGAGAATGGTGGGTCAGGATACCAGTC 5 0.125 No Hit AACACGAAATTGAAGGATAGAAATTTCATCAGATGACAGTTAACTTGTAA 5 0.125 No Hit GCGTATCCAAACTTCTCTGTCCAGCCTTCTTCCCAGAAGCCAATGACTTC 5 0.125 No Hit GTTGGATCGGTAGCACCTTCTTTGTAATAAGCAAAGACCAAACAACCATC 5 0.125 No Hit AGTCTGGTTAGAAGTTTCAGATATGGACACAGTTGAGAGGGGGAAAACGA 5 0.125 No Hit GCCCCTGCGTGCTTTCAAATCATTAAAAAACCGATCCTCGCCTCCTCGCC 5 0.125 No Hit CTCCTCTTTAACTTCTTTTGAGCATGACGGAGACGACGTCGTTGTCAATC 5 0.125 No Hit AAGACTTCAGAATTCAGTAGATTACATCACTGGAGATGATAAAAGAAAAG 5 0.125 No Hit CCATGCAGATACCTTTTACTATTTTTGCCCCCAACTGTGTATCTAATTGT 5 0.125 No Hit GTCCTTTCCTTCACCAAACCCATCTTTAATCGGAGTAAGCAGACTATCAT 5 0.125 No Hit GGACTGCATTCCGGAGGTAGACCTTGGGGAAATCTATTGATATCTTTGCG 5 0.125 No Hit GGGACGAGGAGACAAGAATGGAGGACAAGGCCAACGATGTCATCAAGCAA 5 0.125 No Hit AGGCTCCAATCGCATTATTAATTTGAGTGATCACAGTTGTTGGACCCGCC 5 0.125 No Hit GTCTGGTATATGAGCATCTTGGTACAGAGAAACCCTACACCCTTGTCTCT 5 0.125 No Hit GGAGTCTTTGCTGGCTTTTCATCTTCACTATCTTCCTCGCTCTCTGAGCC 5 0.125 No Hit ACCAGCTTTCTCAAATAAGATCCACAGAGTTGTTGCAGACACTAAAACAC 5 0.125 No Hit GTGCCAAATCGTCCACCCACTTAGGCAACCCAACCGAGGAAGTCGCATAA 5 0.125 No Hit GGATAAGAATCATTCCAACAAAAAGTTTAGGCTGTTGAGCATTAGCTCTG 5 0.125 No Hit CCATGCCACCCGCATCTTGATCATCTTCAACCCATGATTTTCCTTTCCCA 5 0.125 No Hit GTAACCTTAACCCATACTAGCACCTTTGTCTTCATCCCCTCTATAACCGT 5 0.125 No Hit GCTCATATGTCCATACTGGTGGCAGTTCCTGCATACAATGTCTTGGTAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.2 0.0 0.0 0.0 0.0 78-79 0.2875 0.0 0.0 0.0 0.0 80-81 0.4125 0.0 0.0 0.0 0.0 82-83 0.5125 0.0 0.0 0.0 0.0 84-85 0.6375 0.0 0.0 0.0 0.0 86-87 0.75 0.0 0.0 0.0 0.0 88-89 0.8374999999999999 0.0 0.0 0.0 0.0 90-91 0.9125 0.0 0.0 0.0 0.0 92-93 1.0875 0.0 0.0 0.0 0.0 94-95 1.45 0.0 0.0 0.0 0.0 96-97 1.7125 0.0 0.0 0.0 0.0 98-99 2.1125 0.0 0.0 0.0 0.0 100-101 2.55 0.0 0.0 0.0 0.0 102-103 2.6625 0.0 0.0 0.0 0.0 104-105 2.95 0.0 0.0 0.0 0.0 106-107 3.1875 0.0 0.0 0.0 0.0 108-109 3.8625 0.0 0.0 0.0 0.0 110-111 4.35 0.0 0.0 0.0 0.0 112-113 4.987500000000001 0.0 0.0 0.0 0.0 114-115 5.4 0.0 0.0 0.0 0.0 116-117 6.2625 0.0 0.0 0.0 0.0 118-119 6.7875 0.0 0.0 0.0 0.0 120-121 7.3875 0.0 0.0 0.0 0.0 122-123 7.9375 0.0 0.0 0.0 0.0 124-125 8.2625 0.0 0.0 0.0 0.0 126-127 9.0 0.0 0.0 0.0 0.0 128-129 9.725 0.0 0.0 0.0 0.0 130-131 10.3125 0.0 0.0 0.0 0.0 132-133 10.850000000000001 0.0 0.0 0.0 0.0 134-135 11.625 0.0 0.0 0.0 0.0 136-137 12.375 0.0 0.0 0.0 0.0 138-139 13.0625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR26075325 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075325_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.498 37.0 37.0 37.0 37.0 37.0 2 36.4395 37.0 37.0 37.0 37.0 37.0 3 36.398 37.0 37.0 37.0 37.0 37.0 4 36.4575 37.0 37.0 37.0 37.0 37.0 5 36.3845 37.0 37.0 37.0 37.0 37.0 6 36.3035 37.0 37.0 37.0 37.0 37.0 7 36.376 37.0 37.0 37.0 37.0 37.0 8 36.3885 37.0 37.0 37.0 37.0 37.0 9 36.518 37.0 37.0 37.0 37.0 37.0 10-14 36.364 37.0 37.0 37.0 37.0 37.0 15-19 36.3127 37.0 37.0 37.0 37.0 37.0 20-24 36.2675 37.0 37.0 37.0 37.0 37.0 25-29 36.11749999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.0381 37.0 37.0 37.0 37.0 37.0 35-39 35.974599999999995 37.0 37.0 37.0 37.0 37.0 40-44 35.9387 37.0 37.0 37.0 37.0 37.0 45-49 35.8634 37.0 37.0 37.0 37.0 37.0 50-54 35.7476 37.0 37.0 37.0 37.0 37.0 55-59 35.71169999999999 37.0 37.0 37.0 37.0 37.0 60-64 35.7808 37.0 37.0 37.0 37.0 37.0 65-69 35.7083 37.0 37.0 37.0 37.0 37.0 70-74 35.6629 37.0 37.0 37.0 37.0 37.0 75-79 35.6598 37.0 37.0 37.0 37.0 37.0 80-84 35.623900000000006 37.0 37.0 37.0 37.0 37.0 85-89 35.5664 37.0 37.0 37.0 37.0 37.0 90-94 35.498200000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.6431 37.0 37.0 37.0 37.0 37.0 100-104 35.5206 37.0 37.0 37.0 37.0 37.0 105-109 35.4375 37.0 37.0 37.0 37.0 37.0 110-114 35.348400000000005 37.0 37.0 37.0 34.6 37.0 115-119 35.4332 37.0 37.0 37.0 37.0 37.0 120-124 35.2737 37.0 37.0 37.0 32.2 37.0 125-129 35.294000000000004 37.0 37.0 37.0 34.6 37.0 130-134 35.198100000000004 37.0 37.0 37.0 29.8 37.0 135-139 34.9504 37.0 37.0 37.0 25.0 37.0 140-144 35.01155 37.0 37.0 37.0 25.0 37.0 145-149 34.97535 37.0 37.0 37.0 25.0 37.0 150-151 34.553375 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 6.0 15 3.0 16 9.0 17 7.0 18 1.0 19 6.0 20 0.0 21 5.0 22 5.0 23 6.0 24 5.0 25 16.0 26 14.0 27 26.0 28 15.0 29 24.0 30 18.0 31 37.0 32 42.0 33 82.0 34 203.0 35 758.0 36 2517.0 37 195.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.1 22.95 10.325 22.625 2 27.650000000000002 25.924999999999997 29.349999999999998 17.075000000000003 3 22.475 26.724999999999998 32.1 18.7 4 26.55 34.275 20.65 18.525 5 25.724999999999998 37.225 20.25 16.8 6 23.3 36.55 22.275 17.875 7 21.175 22.1 37.15 19.575 8 21.025 25.6 27.224999999999998 26.150000000000002 9 22.825 25.874999999999996 27.450000000000003 23.849999999999998 10-14 25.19 29.189999999999998 25.53 20.09 15-19 24.805 27.72 27.12 20.355 20-24 24.855 28.235 26.58 20.330000000000002 25-29 26.015 27.93 25.435000000000002 20.62 30-34 24.59 27.834999999999997 26.419999999999998 21.154999999999998 35-39 25.94 27.125 26.650000000000002 20.285 40-44 24.295 27.925 26.474999999999998 21.305 45-49 23.965 27.855 27.08 21.099999999999998 50-54 23.115 28.945 27.305 20.635 55-59 24.775 28.525 26.135 20.565 60-64 24.215 27.785 27.215 20.785 65-69 24.445 27.275 27.045 21.235 70-74 23.715 28.68 26.805 20.8 75-79 24.025 28.65 26.575 20.75 80-84 24.495 28.335 25.88 21.29 85-89 25.235000000000003 27.57 26.729999999999997 20.465 90-94 24.84 28.605000000000004 26.085 20.47 95-99 24.585 28.505000000000003 26.619999999999997 20.29 100-104 25.319999999999997 28.754999999999995 25.729999999999997 20.195 105-109 25.130000000000003 28.435 26.275 20.16 110-114 24.73 28.299999999999997 26.924999999999997 20.044999999999998 115-119 25.480000000000004 28.349999999999998 26.345000000000002 19.825 120-124 26.150000000000002 27.584999999999997 26.07 20.195 125-129 26.740000000000002 28.444999999999997 25.305 19.509999999999998 130-134 26.474999999999998 27.474999999999998 26.33 19.72 135-139 26.39 28.32 25.47 19.82 140-144 26.286314315715785 28.291414570728534 26.57132856642832 18.850942547127357 145-149 26.223933590038506 28.289243386507977 26.18892833925089 19.297894684202628 150-151 27.640955119389925 28.653581697712216 24.815601950243778 18.88986123265408 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 1.5 10 0.5 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.5 17 1.0 18 1.0 19 1.0 20 1.0 21 1.5 22 1.5 23 1.0 24 1.5 25 2.5 26 1.5 27 1.5 28 3.0 29 5.0 30 13.0 31 14.5 32 13.0 33 21.0 34 32.0 35 45.0 36 73.0 37 96.0 38 123.0 39 171.0 40 193.5 41 212.5 42 231.0 43 269.0 44 274.0 45 255.0 46 284.0 47 264.5 48 220.0 49 210.0 50 188.5 51 149.0 52 126.0 53 94.5 54 68.0 55 58.5 56 46.5 57 37.5 58 29.0 59 21.0 60 14.0 61 15.0 62 15.5 63 10.0 64 6.5 65 3.5 66 1.5 67 2.0 68 4.5 69 4.0 70 1.5 71 2.0 72 4.5 73 3.5 74 1.0 75 2.5 76 2.0 77 1.0 78 2.0 79 1.5 80 0.0 81 0.0 82 0.0 83 0.5 84 1.0 85 1.5 86 3.5 87 5.0 88 4.5 89 3.5 90 2.5 91 2.5 92 4.0 93 3.0 94 0.5 95 0.5 96 1.5 97 1.5 98 0.5 99 0.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.015 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.74999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 66.37450199203188 41.65 2 20.35856573705179 25.55 3 6.653386454183267 12.525 4 3.745019920318725 9.4 5 1.4741035856573705 4.625 6 0.7569721115537849 2.85 7 0.27888446215139445 1.225 8 0.2390438247011952 1.2 9 0.0 0.0 >10 0.1195219123505976 0.975 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 15 0.375 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 14 0.35000000000000003 No Hit AAACAGGAAAGCTTCGGAAGGCCTGGGATGGAAGCAAAATCATTTATAAT 10 0.25 No Hit CAGAAACTAGCCCTTCTCCACTTCAAATCCTCCCTCTTGGACAGTATTAA 8 0.2 No Hit AGATGGTAGTGACAGGAGAGGCACCGGCTGTTCATGGATACAGCATGCCT 8 0.2 No Hit CTGGGCTACAAGAGGAGGTCTTATCAAAACCGATTGGAGCCAAGCACCGT 8 0.2 No Hit ATGAAGCTGGTTCGAAAACATTAGAGGTTAGATTGAGTCATTCTGGTGAT 8 0.2 No Hit AGCCACGGTTCTAATTTTGGTCCGATTCCCACACAATCACCGATCAGAAG 8 0.2 No Hit TTTACTGGAGAATGTGGGACAAGTCTGGACCATGGTGTTGCTGCCGTGGG 8 0.2 No Hit CTCGTCCAGAAGCATTTCCAAACTCACAACTCTAGCATCCAAACTAAGGA 7 0.17500000000000002 No Hit AGTTGATTGAGTTGTTGCTAAAAGGCCAAAACTTAAGTGAAGATGAAGAA 7 0.17500000000000002 No Hit GACCAAAGTCCCTTTGGAGTGTTAAAATTGTTGATCTAAGCTACCTCTTC 7 0.17500000000000002 No Hit GTTCCCTCCAGAAAACCTTAAGAAACGCCTTGAACAACTGGAGAAGGCCA 7 0.17500000000000002 No Hit GGAGGATCCATGAACAGAGTTCAAGGGTGCTGAGAAGAAGATTGGATGAA 7 0.17500000000000002 No Hit GATACCATGGCAGAGAATCTCAAGAGAGAGCTTTTTGGCTTGCCTCCCCG 7 0.17500000000000002 No Hit GCCATGGAATTTCTGGGGCAATGGGGAAGGTGGGTGCTTTCTTTGGGACA 7 0.17500000000000002 No Hit CCTGCTCTCGCAATCGCTGCTTCTTTGTCTGTCTTTGGGTCGATCCGAAA 6 0.15 No Hit TGGTGCAACAGGTTACCGGTGTTAGATTCGGCAACTCACAGGTTTCAATG 6 0.15 No Hit TGGAGACAGAGAAGTTCTTGACAGTGGATTTCATCCCCCAACTCATGTTC 6 0.15 No Hit GGGAAGGCTGGGCAAGTAACTCCCCACCCTGCAAAGGGAAAAGCTGCGTC 6 0.15 No Hit CCTTTCTCCATTGAAGCTTGATGGCAAGCTGATCTTGATGGGTGTTATTA 6 0.15 No Hit CTTGATCGAAACAGCCACCACCATGGGGTCGATCCCCGATCCAGGCGAGT 6 0.15 No Hit ATTCAAGATGCAGCGCAAGAGGAAATCACGTAGAGGTGGACCTCATTCAA 6 0.15 No Hit GTGGCATGGGGTCCACTTCTTTCATTCATGATGGAGGTCTTCAGAGTGCA 6 0.15 No Hit GCAATTTACCTCTCATTAGCCCTACCAAGAAAGATACAATAATGAGGCGC 6 0.15 No Hit GATTGATTGCAGCCGTTCTTGGCAATAAGTTCTACTGGTGGATCGACCCT 6 0.15 No Hit CCGCTCACCGTCTTCCTTGATAGCAATACCACTTGTTGATTTCTTTCTTT 6 0.15 No Hit CTGAGGTTGCCAGGCTCTTGCTTGATGCCTCCGCTGATCCTAATTCTGTT 6 0.15 No Hit TTACAGTCACAGGACACAGCCTCGGCGCTGCCTTGGCTCTACTAGTAGGC 6 0.15 No Hit CATGGCAAATCGTTGGTGGGCTGGAAATGTTGCCATGAGCGGTGGAGATC 6 0.15 No Hit ACCATTCCTTGCCGATTCTCGGAGTTTGAGAGTGTCCAAACCCGTATTAA 6 0.15 No Hit CTCGCCCTCCAAAAGTGTCGAAATGTTCTTCTAGTTCAGTTCAGGAAGAT 6 0.15 No Hit GCCAAATCACGCAACATCTCATATTTGAAAGGAAAAGTGCCGTCTTGGAT 6 0.15 No Hit GCCGCAGCCTCCTCATCTCCCAAGAGTGGAGGCTTGTGGCAGAACAAAGT 6 0.15 No Hit AGTGGATGTGGGAGGCCCGAGACAAGGACACATGAGTTTCGCAGGTGTTC 6 0.15 No Hit GAAGAAAGCTGATTCAAGTGATGATGACTCTGATTCCGATGAATCCTCTT 5 0.125 No Hit ATCCAATTCGTGCAGGTTCCAAGATCCATGTGAAGCTACAATACTTTGAT 5 0.125 No Hit GGAATTTAAATTATGGAATGACAGTCTGACAGAGAAAGAACAAAATGATG 5 0.125 No Hit GCGTGAATATGATGAGTTCAAGGTTCGAATAAATAGCTTGCCTGATTCTA 5 0.125 No Hit CGGGAACAACATTAGACAATGTGAAGGGAGAAATTGAACTTCGTCACATA 5 0.125 No Hit GAACATACCGTCGTATGGCGTTGTTGAATGTGCGGGAGAGGCTAGTGCTG 5 0.125 No Hit ATTTAGTCCAACCACCATCAATAGTGGGCGTGGTGCTGAATTTGAAGGAG 5 0.125 No Hit ATAAGCAGGGCCATATTGCAGCTGACTGTACAAATGACAAAGCATGCAAC 5 0.125 No Hit CTGCCAGTGAGAATTCCAGAGGTTTGACATCTTCAAAACCAGCAAAATCC 5 0.125 No Hit GGGAGGGGAATAGCGGTGCAGTTGAAGTGCTCAACGGGTTTCGATTCGCT 5 0.125 No Hit CCAAGAACTACGGTCCTGGATCCCGAACCTGCCGAGTGTGTGGAAACCCT 5 0.125 No Hit CAAAAATCGCTCAATACCTGCATACTTTCAACTCATCTCAATCTAGTCCT 5 0.125 No Hit GAGAAACTTGATGAGGACCAGAAGGAACATTTTAGAAAGAACATTGAGGG 5 0.125 No Hit GGAGAATTATATACGAAACTGGCTTGAGGAAAGGATTGGCTTTAAAGCTG 5 0.125 No Hit CACTTTGGCGTCCTAGCTTTAAGTTGCTCTTGGTCACCCTTGGTGAAAAC 5 0.125 No Hit TATCATCACCACCTTCTTCTTTTCTGGTCTTGTGTACCACCATGAAACTA 5 0.125 No Hit CGGGCTATCTGCTGGAATGGCCATTGGCATTGTTGGTGATGCTGGTGTCA 5 0.125 No Hit CTTGTTGATTGGCACTGAAACAACTGGATTGTGTGCTGGTGGGTGTAAAG 5 0.125 No Hit CTTCACATTCCCCCCGCTTCCGCCACCCTCACTTCTAACAAACCCAACAG 5 0.125 No Hit ATTCACCACCCAAAAGTTCTTGATGATAAGGACAGCTGGTCATTGTTCTG 5 0.125 No Hit CCTGATCATTGCCTCTATCTTCTATTATGTTGCGACCAATTACTTCCACC 5 0.125 No Hit ATGAATTCGATGAAACAAAGGCCAAGCTGATTGTGTATTTGCCTTCTGTA 5 0.125 No Hit ACGCCGCCGACCAGGCCGCTGATACTGCCCGCACAGCCGCCGACTCCCTC 5 0.125 No Hit GTATGTTACTGTTCGCAGGCAGAGTGATCTCAGACTCATCATCATCAGCT 5 0.125 No Hit AGAATCTCTTATCTACTCGCCCCCCATCTCTCATTCCTCCTTTTATCTGA 5 0.125 No Hit AGCTCGTCAGGTTTTTGATGAAGCTGAACAATGAGACCGTCTCAATTGAA 5 0.125 No Hit GCCCAGCCTTGGCTCCATGATGATCTGGCCAATTTAATGACCCATTTCCT 5 0.125 No Hit GTTGAAATGGGTCCGGGAGAATTACATGGTATACAATTACTGCAAAGATG 5 0.125 No Hit CTTGAAGATATCGTTCCTTCATCTCACAACTGTGATGTTCCCCATGTTAA 5 0.125 No Hit CTCTGAATTAGCTACCTGTAAAATTGAATCACAATGGGCGAGTCCGTCAC 5 0.125 No Hit TGGCAACTCTGAGTCCGTTGGCCCTCGGATCATCATCCCTGTCGTTGCCT 5 0.125 No Hit TTTAAAGAAGAAGGACCTCTGCTTCCTTTCTATACAGTATGTAATTCCTT 5 0.125 No Hit GTGGAATCCCTCTAAATAAAATCAAGGGCAGCGGACCCGAAGGCCGTATT 5 0.125 No Hit TGGAAGGGACTTCTTGACCCACTCGACGAGAATCTCCGGCGAGAAGTTGT 5 0.125 No Hit GATAAATAGGTATCCTCTAGGAAGGTTTTGCCGGGGTGTTCTCTCCTATC 5 0.125 No Hit AATCGCCGCAGCTATCCAGCAGTACATGAAGGAAGGCCGCCGTTCGATCA 5 0.125 No Hit ATGAGATTGATGACTTTCCAGTTCGTGTGCCAAGAGTGGCACCACTTGTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.2125 0.0 0.0 0.0 0.0 80-81 0.32499999999999996 0.0 0.0 0.0 0.0 82-83 0.4125 0.0 0.0 0.0 0.0 84-85 0.5375000000000001 0.0 0.0 0.0 0.0 86-87 0.65 0.0 0.0 0.0 0.0 88-89 0.7375 0.0 0.0 0.0 0.0 90-91 0.8125 0.0 0.0 0.0 0.0 92-93 1.0 0.0 0.0 0.0 0.0 94-95 1.35 0.0 0.0 0.0 0.0 96-97 1.6124999999999998 0.0 0.0 0.0 0.0 98-99 1.9875 0.0 0.0 0.0 0.0 100-101 2.4 0.0 0.0 0.0 0.0 102-103 2.5125 0.0 0.0 0.0 0.0 104-105 2.8 0.0 0.0 0.0 0.0 106-107 3.0875 0.0 0.0 0.0 0.0 108-109 3.7625 0.0 0.0 0.0 0.0 110-111 4.300000000000001 0.0 0.0 0.0 0.0 112-113 4.975 0.0 0.0 0.0 0.0 114-115 5.4 0.0 0.0 0.0 0.0 116-117 6.2875 0.0 0.0 0.0 0.0 118-119 6.8125 0.0 0.0 0.0 0.0 120-121 7.3875 0.0 0.0 0.0 0.0 122-123 7.95 0.0 0.0 0.0 0.0 124-125 8.2625 0.0 0.0 0.0 0.0 126-127 9.0 0.0 0.0 0.0 0.0 128-129 9.725 0.0 0.0 0.0 0.0 130-131 10.3125 0.0 0.0 0.0 0.0 132-133 10.875 0.0 0.0 0.0 0.0 134-135 11.6625 0.0 0.0 0.0 0.0 136-137 12.425 0.0 0.0 0.0 0.0 138-139 13.1375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra Read 2199100 spots for SRR26075325.sra Written 2199100 spots for SRR26075325.sra Read 2199097 spots for SRR26075325.sra Written 2199097 spots for SRR26075325.sra SRR ids: ['SRR26075325.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_uuk8h2qa SRR26075325.sra spots: 43981943 blocks: [[1, 2199097], [2199098, 4398194], [4398195, 6597291], [6597292, 8796388], [8796389, 10995485], [10995486, 13194582], [13194583, 15393679], [15393680, 17592776], [17592777, 19791873], [19791874, 21990970], [21990971, 24190067], [24190068, 26389164], [26389165, 28588261], [28588262, 30787358], [30787359, 32986455], [32986456, 35185552], [35185553, 37384649], [37384650, 39583746], [39583747, 41782843], [41782844, 43981943]] SRR26075325 file size 16244652 SRR26075325 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075325 SRR26075325_1.fastq SRR26075325_2.fastq Input file: SRR26075325_1.fastq Paired file: SRR26075325_2.fastq trimmed: SRR26075325-trimmed-pair1.fastq, SRR26075325-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 19:18:49 2025 >> started Tue Feb 11 19:20:01 2025 >> done (71.922s) 43981943 read pairs processed; of these: 273 ( 0.00%) short read pairs filtered out after trimming by size control 206905 ( 0.47%) empty read pairs filtered out after trimming by size control 43774765 (99.53%) read pairs available; of these: 7719939 (17.64%) trimmed read pairs available after processing 36054826 (82.36%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 15 0.00% 19 36 0.00% 20 32 0.00% 21 39 0.00% 22 35 0.00% 23 37 0.00% 24 52 0.00% 25 35 0.00% 26 62 0.00% 27 82 0.00% 28 72 0.00% 29 73 0.00% 30 55 0.00% 31 85 0.00% 32 64 0.00% 33 125 0.00% 34 78 0.00% 35 85 0.00% 36 106 0.00% 37 124 0.00% 38 137 0.00% 39 185 0.00% 40 155 0.00% 41 183 0.00% 42 151 0.00% 43 176 0.00% 44 223 0.00% 45 216 0.00% 46 259 0.00% 47 268 0.00% 48 379 0.00% 49 429 0.00% 50 425 0.00% 51 468 0.00% 52 574 0.00% 53 516 0.00% 54 601 0.00% 55 802 0.00% 56 816 0.00% 57 906 0.00% 58 1003 0.00% 59 1158 0.00% 60 1365 0.00% 61 1463 0.00% 62 1805 0.00% 63 2122 0.00% 64 2323 0.01% 65 2514 0.01% 66 2773 0.01% 67 3062 0.01% 68 3445 0.01% 69 4027 0.01% 70 4732 0.01% 71 5199 0.01% 72 6036 0.01% 73 7032 0.02% 74 7678 0.02% 75 8717 0.02% 76 9944 0.02% 77 10739 0.02% 78 11628 0.03% 79 12625 0.03% 80 14340 0.03% 81 16088 0.04% 82 17887 0.04% 83 20199 0.05% 84 22605 0.05% 85 25004 0.06% 86 26374 0.06% 87 28716 0.07% 88 30795 0.07% 89 32508 0.07% 90 34675 0.08% 91 37851 0.09% 92 40137 0.09% 93 44220 0.10% 94 48298 0.11% 95 51310 0.12% 96 53167 0.12% 97 57224 0.13% 98 59468 0.14% 99 62239 0.14% 100 63800 0.15% 101 66697 0.15% 102 70760 0.16% 103 73043 0.17% 104 77147 0.18% 105 81879 0.19% 106 85406 0.20% 107 86628 0.20% 108 90827 0.21% 109 92520 0.21% 110 92875 0.21% 111 95999 0.22% 112 99661 0.23% 113 102306 0.23% 114 105312 0.24% 115 111203 0.25% 116 114337 0.26% 117 117225 0.27% 118 120823 0.28% 119 122545 0.28% 120 123484 0.28% 121 125105 0.29% 122 126251 0.29% 123 130787 0.30% 124 134531 0.31% 125 137088 0.31% 126 141008 0.32% 127 144346 0.33% 128 147511 0.34% 129 148600 0.34% 130 151504 0.35% 131 151873 0.35% 132 155100 0.35% 133 156327 0.36% 134 159748 0.36% 135 162810 0.37% 136 164460 0.38% 137 167487 0.38% 138 171173 0.39% 139 174122 0.40% 140 175059 0.40% 141 176390 0.40% 142 178922 0.41% 143 179637 0.41% 144 184408 0.42% 145 184807 0.42% 146 185871 0.42% 147 188846 0.43% 148 190006 0.43% 149 192865 0.44% 150 195164 0.45% 151 36054826 82.36% 43774765 reads passed initial QC criterion=sequence-density sequence-density=0.19 sequence-density-rank=1 fanout-score=3.53 fanout-score-rank=29 prefix-density=0.29 prefix-fanout=2.3 sequence=CACTTGCAGCCATTCTCAGCACC criterion=fanout-score sequence-density=0.02 sequence-density-rank=38 fanout-score=199.67 fanout-score-rank=1 prefix-density=0.42 prefix-fanout=11.0 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.22 sequence-density-rank=1 fanout-score=2.92 fanout-score-rank=33 prefix-density=0.23 prefix-fanout=2.9 sequence=ATGTACCCTGACTTAGGTTTCTCAGA criterion=fanout-score sequence-density=0.09 sequence-density-rank=21 fanout-score=348.44 fanout-score-rank=1 prefix-density=1.00 prefix-fanout=33.2 sequence=AAGAAGAAGAAA SRR26075325 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 19:20:41 Started mapping on | Feb 11 19:20:42 Finished on | Feb 11 19:26:41 Mapping speed, Million of reads per hour | 438.97 Number of input reads | 43774765 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 39841317 Uniquely mapped reads % | 91.01% Average mapped length | 291.09 Number of splices: Total | 32568235 Number of splices: Annotated (sjdb) | 31721223 Number of splices: GT/AG | 31966731 Number of splices: GC/AG | 451146 Number of splices: AT/AC | 34034 Number of splices: Non-canonical | 116324 Mismatch rate per base, % | 0.42% Deletion rate per base | 0.03% Deletion average length | 2.97 Insertion rate per base | 0.02% Insertion average length | 2.66 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1439773 % of reads mapped to multiple loci | 3.29% Number of reads mapped to too many loci | 117897 % of reads mapped to too many loci | 0.27% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.09% % of reads unmapped: other | 0.34% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2493675 2493675 2493675 N_multimapping 1439773 1439773 1439773 N_noFeature 1268903 39376235 1534301 N_ambiguous 492915 2976 291339 UnstrandedReadsAssigned:38079499 PositiveStrandReadsAssigned:462106 NegativeStrandReadsAssigned:38015677 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075325 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075325-trimmed-pair1.fastq SRR26075325-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 43,774,765 reads, 38,872,664 reads pseudoaligned [quant] estimated average fragment length: 211.271 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,268 rounds 52401 SRR26075325.ke.tsv 34699 SRR26075325.se.tsv 87100 total ==> SRR26075325.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1807.73 6419 81.7512 Potri.005G024800.1.v4.1 1035 824.729 3083 86.0642 Potri.004G059700.1.v4.1 961 750.744 0 0 Potri.007G009000.2.v4.1 1416 1205.73 0 0 Potri.003G141000.2.v4.1 2943 2732.73 1493.35 12.5813 Potri.016G087400.1.v4.1 270 93.9199 3881 951.362 Potri.015G069301.1.v4.1 564 355.915 0 0 Potri.010G195200.1.v4.1 1773 1562.73 775 11.4177 Potri.012G127500.1.v4.1 977 766.739 6859 205.955 ==> SRR26075325.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 363 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 476 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 28 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1096 SRR26075325 completed mapping pipeline successfully