Starting /dee2/code/volunteer_pipeline.sh SRR26075326 current disk space = 3053179260928 free memory = 1579868196 SRR26075326 SRAfilesize bba1221d1f3ad12d5243a916a75f1e37 SRR26075326.sra SRR26075326.sra file validated SRR26075326 is paired end SRR26075326 is conventional basespace SRR26075326 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075326_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.53625 37.0 37.0 37.0 37.0 37.0 2 36.6435 37.0 37.0 37.0 37.0 37.0 3 36.6005 37.0 37.0 37.0 37.0 37.0 4 36.713 37.0 37.0 37.0 37.0 37.0 5 36.67 37.0 37.0 37.0 37.0 37.0 6 36.726 37.0 37.0 37.0 37.0 37.0 7 36.6475 37.0 37.0 37.0 37.0 37.0 8 36.6445 37.0 37.0 37.0 37.0 37.0 9 36.636 37.0 37.0 37.0 37.0 37.0 10-14 36.66525 37.0 37.0 37.0 37.0 37.0 15-19 36.619800000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.5567 37.0 37.0 37.0 37.0 37.0 25-29 36.433299999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.4331 37.0 37.0 37.0 37.0 37.0 35-39 36.3468 37.0 37.0 37.0 37.0 37.0 40-44 36.3078 37.0 37.0 37.0 37.0 37.0 45-49 36.234700000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.2457 37.0 37.0 37.0 37.0 37.0 55-59 36.1594 37.0 37.0 37.0 37.0 37.0 60-64 36.0535 37.0 37.0 37.0 37.0 37.0 65-69 35.9801 37.0 37.0 37.0 37.0 37.0 70-74 35.9971 37.0 37.0 37.0 37.0 37.0 75-79 35.9784 37.0 37.0 37.0 37.0 37.0 80-84 35.909299999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.793600000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.811400000000006 37.0 37.0 37.0 37.0 37.0 95-99 35.738299999999995 37.0 37.0 37.0 37.0 37.0 100-104 35.7564 37.0 37.0 37.0 37.0 37.0 105-109 35.679199999999994 37.0 37.0 37.0 37.0 37.0 110-114 35.5933 37.0 37.0 37.0 37.0 37.0 115-119 35.5034 37.0 37.0 37.0 34.6 37.0 120-124 35.52419999999999 37.0 37.0 37.0 37.0 37.0 125-129 35.3647 37.0 37.0 37.0 37.0 37.0 130-134 35.3174 37.0 37.0 37.0 34.6 37.0 135-139 35.1277 37.0 37.0 37.0 29.8 37.0 140-144 34.9839 37.0 37.0 37.0 27.4 37.0 145-149 34.9718 37.0 37.0 37.0 25.0 37.0 150-151 34.98825 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 1.0 20 2.0 21 4.0 22 3.0 23 5.0 24 6.0 25 15.0 26 7.0 27 16.0 28 17.0 29 29.0 30 31.0 31 48.0 32 64.0 33 87.0 34 178.0 35 447.0 36 2839.0 37 200.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.16218930454431 16.695957820738137 7.9086115992970125 37.23324127542054 2 18.025 15.85 34.949999999999996 31.175000000000004 3 17.325 18.2 28.525 35.949999999999996 4 20.95 24.575 24.7 29.775000000000002 5 25.0 28.975 24.45 21.575 6 23.974999999999998 31.474999999999998 22.525000000000002 22.025 7 15.975 29.95 38.025 16.05 8 18.7 27.800000000000004 31.674999999999997 21.825 9 17.7 24.575 34.55 23.175 10-14 19.930996549827494 29.706485324266215 27.94139706985349 22.421121056052804 15-19 19.77 27.67 28.24 24.32 20-24 18.35 28.03 28.65 24.97 25-29 20.175 28.694999999999997 27.865000000000002 23.265 30-34 19.439999999999998 27.99 27.49 25.080000000000002 35-39 20.085 27.93 27.750000000000004 24.235 40-44 19.89 29.189999999999998 26.96 23.96 45-49 20.294999999999998 27.529999999999998 28.205000000000002 23.97 50-54 20.615 27.985 27.36 24.04 55-59 20.215 28.76 27.57 23.455000000000002 60-64 20.105 27.71 28.23 23.955000000000002 65-69 19.755 28.58 27.26 24.404999999999998 70-74 19.8 28.77 27.43 24.0 75-79 20.165 27.49 28.23 24.115000000000002 80-84 19.835 26.755000000000003 28.98 24.43 85-89 21.18 27.445000000000004 27.43 23.945 90-94 20.73 27.279999999999998 27.505000000000003 24.485 95-99 21.575 26.715 27.694999999999997 24.015 100-104 20.265 27.405 28.305000000000003 24.025 105-109 20.979999999999997 27.169999999999998 27.825 24.025 110-114 21.07 27.6 25.990000000000002 25.34 115-119 21.105 26.674999999999997 27.744999999999997 24.474999999999998 120-124 21.575 27.905 26.995 23.525 125-129 22.015 25.835 27.555000000000003 24.595 130-134 22.38 27.065 26.8 23.755000000000003 135-139 21.82 26.584999999999997 27.62 23.974999999999998 140-144 21.925 28.1 26.52 23.455000000000002 145-149 22.259999999999998 26.619999999999997 25.929999999999996 25.19 150-151 22.05 26.9625 26.05 24.9375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.5 9 1.0 10 0.5 11 0.0 12 0.5 13 1.0 14 0.5 15 0.5 16 1.0 17 1.0 18 1.0 19 1.0 20 1.0 21 1.5 22 2.5 23 2.0 24 1.0 25 2.0 26 6.5 27 8.5 28 8.5 29 11.5 30 19.5 31 26.5 32 26.5 33 31.0 34 41.0 35 53.0 36 66.5 37 99.0 38 131.5 39 154.5 40 193.0 41 235.5 42 244.5 43 240.5 44 267.5 45 288.5 46 280.0 47 251.0 48 209.0 49 176.0 50 167.5 51 142.0 52 121.5 53 106.5 54 83.0 55 59.5 56 47.0 57 44.0 58 34.0 59 23.5 60 15.0 61 11.5 62 7.5 63 7.5 64 7.0 65 6.5 66 3.5 67 7.5 68 8.5 69 3.5 70 2.0 71 1.5 72 0.0 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42500000000000004 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.0 #Duplication Level Percentage of deduplicated Percentage of total 1 61.86440677966102 36.5 2 21.440677966101696 25.3 3 9.067796610169491 16.05 4 4.364406779661016 10.299999999999999 5 1.6101694915254237 4.75 6 0.7627118644067797 2.7 7 0.38135593220338987 1.575 8 0.211864406779661 1.0 9 0.1271186440677966 0.675 >10 0.1694915254237288 1.15 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTGCGTTATCTCGTAT 14 0.35000000000000003 TruSeq Adapter, Index 13 (97% over 36bp) CCCTTCTCTGTCTCGGACGCACAAACAAAACAATGTTGTTCACTTTCTAT 11 0.27499999999999997 No Hit CTTCTTCTTCTTCCTTTGGAGCTTCGACTGCAACTTCTGGTTCTGGTGCC 11 0.27499999999999997 No Hit CAGGTGTGTAGCCAAAGGTTACTTCAACAAACTGAAGCACGGGTGGTGGG 10 0.25 No Hit GCCTAACTTGAGAGTGTCAATGGGGCAAGTCTCTTGCTTCGGTGGTGTTG 9 0.22499999999999998 No Hit GTGGCCTACAAACAATGACTTGGTAGTGAATGCATAAGGTTGCACATCCA 9 0.22499999999999998 No Hit CTTCGGATGACATATAGAAAGCAGTAACAGTTCCGGCAGAGTCACCCTCT 9 0.22499999999999998 No Hit ATCATGGGAGTGCACATTCATTCACTGCATTTCAAGGCACCTCAGAATCA 8 0.2 No Hit CCACCTAAGATCCTCAGAACTGCCCCAACCCAAAATGCATAACATGTGTC 8 0.2 No Hit GTAGACTGGTAAAGTGTCTCCTCGAAAAGAATGGCACCAGAAATGTATTC 8 0.2 No Hit GACTCCTTCCATGAATAACATGGCAAATCAGCAAGACAGCTTCTGTGGAA 8 0.2 No Hit CTTCATTCAAGATTGCACTCGAGTCACTATCCGATAACCCATCTTTGAAG 8 0.2 No Hit CAGGGAAATGTAGTGTGAACCAAATAGGTCTCCCCATCTAGGCCGACTCC 7 0.17500000000000002 No Hit CACCATTGATTTGCACGCCTCCAACAAGGTGAGCAGCAATACCTGAGAAT 7 0.17500000000000002 No Hit CGGACTTCTTCCTCCTCCTTTCTTTCGTTTGCTGTACAAAACTACTATGT 7 0.17500000000000002 No Hit CCTTTGAGGAAGGCCTGATCATGACCTGACGCTTCCCACGTTTCTCTGCG 7 0.17500000000000002 No Hit CTCGAGAGAGGCGATATCCATGATCTTATCCTCCACCGGCTTAGCGCAAT 7 0.17500000000000002 No Hit CTCAAGAGCAATGGGTATAATGATACTGATGTTGAGAAGCTTAGCCTTAA 7 0.17500000000000002 No Hit AACCTACTCACAATTTCAATCATTTTTTTTTCTCAATCCATAATTTGCAC 7 0.17500000000000002 No Hit AAATGGAGAATTTGAAGGAAACCAAGCACGTTGTGGGCAGTGAAAATTAA 7 0.17500000000000002 No Hit TGAGCATTGCCACTAAACAAATCCGTTCCTCTAACTAGCATCTAATAGCA 7 0.17500000000000002 No Hit CCGCCAAGTTATTTTCCATCCCCCCTGAGAAGTTTAAAGCAGAAATCTTC 6 0.15 No Hit GTTCCCAATCAGAATGATAACTATGTTCTTGTCGGCATGGCTACGCAGTT 6 0.15 No Hit GTCATCAACAAGGTTCGAGGAATTGCTCTTGACCTGACAAAATGTTCTGA 6 0.15 No Hit CCGAATTTCTTGATAAGTTCATTTGCAATCAACTCATTTGCGCTGTCTGA 6 0.15 No Hit CTCAAGGAGGATTGGACCTCTAGATCCAACGGTCAAAGACGAGTTGTTGT 6 0.15 No Hit TCCCCTTTGGGTGCCTGAACAGAGTGCGGTGTTGCACAAAGATAACCTCT 6 0.15 No Hit GTATTCGTTGCTATTGCAAGCATTCATTATATCTTATATGCTGACAACTT 6 0.15 No Hit GAAAACATCAGCAGGTTTTCCACCGCCTAAGACTTTGTGGACGGGTTTCT 6 0.15 No Hit GTTATATTCTAGTTGCCGAAAACATTATACTATTGGTTGTAAAAAGATAT 6 0.15 No Hit CATAGGGCCAACATCATATTCCCTTGAATCACTTTCTGTTCCAAATTCTA 6 0.15 No Hit GTCATTAACTTTATGACATGTCTCCACAGCCCACAAATCAATTCTTGGCA 6 0.15 No Hit CCGAGGATCATCAGAGCCAGAGGCATCAGGATCAGACAAAGATGATGTCT 6 0.15 No Hit AACAGGAGAGACATCCCCACTATATTCACACACATGCAACAAACATCTTC 6 0.15 No Hit CGGCAAACTGGGAAAGCACACTCATTGCAAGCAACAAAGATGTCGCCATT 6 0.15 No Hit CTCATCGTCTTCGCATCCCCCGTCCACGGCCACGGAAACCACCTCCCCCA 6 0.15 No Hit AACCATCAATGCTGTCATTGTGATAACCAAAATAACCATTGCCTCGCCCC 6 0.15 No Hit GGCACAATCACATCCAATAAGTATGGCCCGGGAGTGTCCAACATTTTTTG 6 0.15 No Hit CTAATATTTAAGAATTTACAGGAATAAAATGGAGTCACTAAGCTATGATT 6 0.15 No Hit CCCCAAGGCATTCTCGCTTTTTACAATAAAGCTAAAATCATTATTTTACA 5 0.125 No Hit TGTGCCAGGACGGAACCTACTATAAAATTCAGTGAAGTCAAGCCGAAAGA 5 0.125 No Hit GTTCCAAGAAACCTTCAGGCAGCAAAGAATCCAAATCAGGGTCTCCCTGT 5 0.125 No Hit GTCCTGCATTGTCCAACCTTCTTCTGGCATCTTCTGAGCTTTGGCAACAA 5 0.125 No Hit GGGCTGACCAGGAAGCTGGTCCGCTACATACAATGTCCAGCTGTGGCCCC 5 0.125 No Hit AACCGGTTGGGAGGGGGAAGGTCTGGAAAATGGGGGAAGATGAGAGGAAG 5 0.125 No Hit GGAAAAAAAAAAGTAATCATCTTTAAGAACTGGCCAGATCTCTCATGCCA 5 0.125 No Hit CCTGTTTCACGACAGCCATTGCAAACATATGCTTTGCGTTTAGTACGAAT 5 0.125 No Hit GTTATCAGTTCCATCACGCTCATGTATCATGCCAGTCGAGGAAGAGTTCG 5 0.125 No Hit AGCCCGACACCATCATCATAAACTGATATGTTAGGTCCTGGTTCGAAGTC 5 0.125 No Hit CTTCAAAAACATCTCTCACTCTCAGCAGTTGAAGCCTTTGCAAATAGAGA 5 0.125 No Hit ACCACCGTCCTGCTGTCTTAATCGACCAACACCCTTTGTGGGTTCTAGGT 5 0.125 No Hit CTCTACTAAAAGCAGCACACACACCAGAATGTGCAAGAGCAAAGATGATG 5 0.125 No Hit GCCTCTTTGTGCTGATAACCTCACCTCTGGCCTTAGCCTCTGCCTTCAGT 5 0.125 No Hit CCCTACTAAACCATTCCATACCTCTTCCTATCCAGCCAGGCAATGCTAAA 5 0.125 No Hit TCTTTGAGGGGTTCCAAAAGCCTGACAGCAATATCAAGACCATTGTTAGC 5 0.125 No Hit CTCCATAGCTGCCCCAAGCTCATCAGGTGACATCAGGCTGATACCATTAA 5 0.125 No Hit ATCCCAAAACAACTTGCTTCATGTTGATAACAAAATTGGACGTCCTTGTA 5 0.125 No Hit ATCGTTAAGGAAGTCTCCGTATGCTTTATTTCGAACGTAAAAATCAGAAG 5 0.125 No Hit ATTCACCATAGTATAAGGTGTCCAGTGCAAATGATGCGTCCCACTCGAGC 5 0.125 No Hit CTACCATAGATATTAAACTTAACCCGATAGGACATCCTGTTCCATGTTCT 5 0.125 No Hit CCTTGGGCCCTTCTTCTTCCGGTGGTGGGTCAGTCTGTGGAGTCTTGTGG 5 0.125 No Hit CCAGAACGACCAATTCGATGAATGTAAAGCTCTCGATTGTTTGGAAGGTC 5 0.125 No Hit GGGAGCAAGGCAGTTGGTAGTGCAACTAGCATTGGATACAATGTTAAGAT 5 0.125 No Hit CCTGATTATTAAGAAGTAAACTAATTACTAGCAACATATTTCACAAAAAA 5 0.125 No Hit CAACTACTTGAGAGAGAGAAGAGAAAAGTGATGATCGAGTCGGAGCTGAT 5 0.125 No Hit GTTCTGTAGTAATATGCCTCTTTGGTTGTAGGAGTATTTTCTGGGTAAAT 5 0.125 No Hit GGCCCAAAATGCCAACATTCTAGCATGAGCTGATTTAAAATGTCAAGTTC 5 0.125 No Hit CCATCCTCAGAGATATCAATCAGCTGGTAGTCAGTACGGGTGACGTGGGG 5 0.125 No Hit GTGAAGATCTATGACAACATTCGAGGCTTCCACTGAGTATAGTATGGATT 5 0.125 No Hit GGGCAAAGTACAAGAATGTTGGATCGGTAGCACCTTCTTTGTAATAAGCA 5 0.125 No Hit AATGTCAGTGTTACGGTGCTTCTGATCTCCATATCTGATGCAGTCAGCTA 5 0.125 No Hit CTGTGTACTCTGCCCACTCTCTATCTCTCGCTCGCTATCTGTGAGATTGA 5 0.125 No Hit CATCTCTACACAGACTCTCCTCTCAAATCAACCGCATTCCTTCTCTCTCT 5 0.125 No Hit GGTGAACAGGTTCGGCTGGGATCCGACTGCGGCGGTGAAGACTTCGTTGC 5 0.125 No Hit GCTTAACGGTGGACCCTCCATCCGCAAACTCTACCGAGGGTAGAGCTCAG 5 0.125 No Hit ATAGAAAGTACTTTACAGGGCGAGCACAGTTCATGGTCTTCACTCTTCAA 5 0.125 No Hit GTATACTTAGGTTTCCAATGACAGTGTCACCCCTTCCTTTTTGTCATCGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0125 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.2375 0.0 0.0 0.0 0.0 86-87 0.38749999999999996 0.0 0.0 0.0 0.0 88-89 0.5625 0.0 0.0 0.0 0.0 90-91 0.625 0.0 0.0 0.0 0.0 92-93 0.7 0.0 0.0 0.0 0.0 94-95 0.8625 0.0 0.0 0.0 0.0 96-97 1.1625 0.0 0.0 0.0 0.0 98-99 1.375 0.0 0.0 0.0 0.0 100-101 2.0625 0.0 0.0 0.0 0.0 102-103 2.2750000000000004 0.0 0.0 0.0 0.0 104-105 2.5625 0.0 0.0 0.0 0.0 106-107 3.0625 0.0 0.0 0.0 0.0 108-109 3.4749999999999996 0.0 0.0 0.0 0.0 110-111 3.675 0.0 0.0 0.0 0.0 112-113 3.875 0.0 0.0 0.0 0.0 114-115 4.15 0.0 0.0 0.0 0.0 116-117 4.475 0.0 0.0 0.0 0.0 118-119 4.95 0.0 0.0 0.0 0.0 120-121 5.725 0.0 0.0 0.0 0.0 122-123 6.5125 0.0 0.0 0.0 0.0 124-125 7.262499999999999 0.0 0.0 0.0 0.0 126-127 7.7125 0.0 0.0 0.0 0.0 128-129 8.412500000000001 0.0 0.0 0.0 0.0 130-131 9.5125 0.0 0.0 0.0 0.0 132-133 10.5625 0.0 0.0 0.0 0.0 134-135 11.0625 0.0 0.0 0.0 0.0 136-137 11.75 0.0 0.0 0.0 0.0 138-139 12.4625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCCTTC 10 0.006830828 145.0 1 >>END_MODULE SRR26075326 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075326_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2315 37.0 37.0 37.0 37.0 37.0 2 36.246 37.0 37.0 37.0 37.0 37.0 3 36.327 37.0 37.0 37.0 37.0 37.0 4 36.2455 37.0 37.0 37.0 37.0 37.0 5 36.305 37.0 37.0 37.0 37.0 37.0 6 36.25 37.0 37.0 37.0 37.0 37.0 7 36.249 37.0 37.0 37.0 37.0 37.0 8 36.227 37.0 37.0 37.0 37.0 37.0 9 36.217 37.0 37.0 37.0 37.0 37.0 10-14 36.1466 37.0 37.0 37.0 37.0 37.0 15-19 36.1183 37.0 37.0 37.0 37.0 37.0 20-24 36.037600000000005 37.0 37.0 37.0 37.0 37.0 25-29 35.9206 37.0 37.0 37.0 37.0 37.0 30-34 35.759499999999996 37.0 37.0 37.0 37.0 37.0 35-39 35.7584 37.0 37.0 37.0 37.0 37.0 40-44 35.71169999999999 37.0 37.0 37.0 37.0 37.0 45-49 35.6763 37.0 37.0 37.0 37.0 37.0 50-54 35.613099999999996 37.0 37.0 37.0 37.0 37.0 55-59 35.6254 37.0 37.0 37.0 37.0 37.0 60-64 35.6534 37.0 37.0 37.0 37.0 37.0 65-69 35.6068 37.0 37.0 37.0 37.0 37.0 70-74 35.5596 37.0 37.0 37.0 37.0 37.0 75-79 35.46489999999999 37.0 37.0 37.0 37.0 37.0 80-84 35.449 37.0 37.0 37.0 37.0 37.0 85-89 35.4305 37.0 37.0 37.0 37.0 37.0 90-94 35.4072 37.0 37.0 37.0 37.0 37.0 95-99 35.3728 37.0 37.0 37.0 37.0 37.0 100-104 35.3519 37.0 37.0 37.0 37.0 37.0 105-109 35.2436 37.0 37.0 37.0 32.2 37.0 110-114 35.2108 37.0 37.0 37.0 29.8 37.0 115-119 35.2078 37.0 37.0 37.0 32.2 37.0 120-124 35.02290000000001 37.0 37.0 37.0 25.0 37.0 125-129 35.0929 37.0 37.0 37.0 29.8 37.0 130-134 35.021100000000004 37.0 37.0 37.0 27.4 37.0 135-139 34.8583 37.0 37.0 37.0 25.0 37.0 140-144 34.81179999999999 37.0 37.0 37.0 25.0 37.0 145-149 34.839800000000004 37.0 37.0 37.0 25.0 37.0 150-151 34.548500000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 7.0 14 11.0 15 8.0 16 4.0 17 7.0 18 10.0 19 6.0 20 5.0 21 5.0 22 12.0 23 6.0 24 18.0 25 17.0 26 20.0 27 18.0 28 15.0 29 17.0 30 16.0 31 37.0 32 55.0 33 78.0 34 187.0 35 734.0 36 2525.0 37 182.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.75 22.225 10.65 23.375 2 29.4 26.400000000000002 28.525 15.675 3 25.224999999999998 27.950000000000003 28.225 18.6 4 27.250000000000004 32.875 22.1 17.775 5 25.45 35.575 21.65 17.325 6 24.825 37.974999999999994 20.125 17.075000000000003 7 24.775 21.275 35.199999999999996 18.75 8 21.85 24.875 28.975 24.3 9 24.474999999999998 24.775 30.599999999999998 20.150000000000002 10-14 25.72 29.435 24.01 20.835 15-19 24.375 28.24 26.33 21.055 20-24 25.03 27.67 26.52 20.78 25-29 26.16 28.24 25.740000000000002 19.86 30-34 25.52 28.505000000000003 26.235000000000003 19.74 35-39 25.52 28.03 26.474999999999998 19.975 40-44 25.09 28.384999999999998 25.974999999999998 20.549999999999997 45-49 24.709999999999997 28.494999999999997 26.650000000000002 20.145 50-54 24.01 28.634999999999998 27.18 20.175 55-59 25.385 28.875 26.19 19.55 60-64 26.14 27.744999999999997 25.72 20.395 65-69 25.505 28.785 25.380000000000003 20.330000000000002 70-74 24.95 28.515 26.145000000000003 20.39 75-79 24.93 29.099999999999998 25.985000000000003 19.985 80-84 25.435000000000002 28.599999999999998 26.095000000000002 19.869999999999997 85-89 25.695 27.765 26.450000000000003 20.09 90-94 26.015 28.67 25.840000000000003 19.475 95-99 24.834999999999997 28.71 26.525 19.93 100-104 25.395 29.189999999999998 26.215 19.2 105-109 24.745 28.560000000000002 26.165 20.53 110-114 25.91 27.500000000000004 26.68 19.91 115-119 25.779999999999998 28.449999999999996 26.27 19.5 120-124 25.545 29.104999999999997 25.915 19.435 125-129 26.16 28.99 25.319999999999997 19.53 130-134 26.46 28.99 25.319999999999997 19.23 135-139 25.985000000000003 27.495000000000005 27.150000000000002 19.37 140-144 28.355000000000004 27.325 25.855 18.465 145-149 27.975 27.91 25.929999999999996 18.185000000000002 150-151 27.750000000000004 27.6125 25.587500000000002 19.05 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 1.0 7 0.0 8 0.0 9 1.0 10 1.0 11 0.0 12 0.5 13 1.0 14 0.5 15 1.0 16 2.0 17 1.0 18 1.5 19 2.5 20 1.5 21 1.0 22 1.0 23 2.5 24 2.0 25 2.5 26 4.0 27 2.0 28 2.0 29 5.5 30 9.5 31 8.5 32 8.5 33 14.5 34 14.0 35 24.0 36 52.5 37 81.5 38 118.5 39 143.5 40 172.5 41 207.5 42 248.0 43 285.0 44 272.0 45 294.5 46 315.0 47 278.0 48 249.5 49 227.0 50 200.0 51 162.0 52 125.0 53 92.5 54 72.5 55 56.5 56 41.0 57 35.5 58 23.0 59 17.0 60 13.0 61 6.0 62 8.5 63 10.0 64 7.5 65 4.5 66 1.5 67 2.5 68 3.0 69 1.5 70 1.0 71 1.0 72 2.0 73 3.0 74 2.0 75 0.0 76 1.0 77 1.5 78 2.0 79 2.0 80 1.5 81 2.5 82 1.5 83 1.0 84 2.5 85 2.0 86 3.5 87 3.0 88 0.0 89 0.5 90 0.5 91 1.0 92 1.0 93 0.5 94 1.5 95 1.0 96 0.5 97 0.5 98 0.0 99 0.0 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.25 #Duplication Level Percentage of deduplicated Percentage of total 1 63.85892116182572 38.475 2 20.456431535269708 24.65 3 8.464730290456432 15.299999999999999 4 3.9834024896265556 9.6 5 1.6597510373443984 5.0 6 0.7883817427385892 2.85 7 0.37344398340248963 1.575 8 0.16597510373443983 0.8 9 0.04149377593360996 0.22499999999999998 >10 0.2074688796680498 1.525 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 20 0.5 No Hit ATTCTCAGGAAGCCACTCCCTGTCATCTTCTGCAACTCTACCCTTTCTTA 11 0.27499999999999997 No Hit GGCATGGGTCAGCAAAGTTAGCTCGTCAGGCGCAGAGCAAGGAGAAAACT 10 0.25 No Hit GTAAAACCTCCCAAAGTAAAGCCGCCACCAATCGTGAAGCCACCAAAGCC 10 0.25 No Hit GGAGGAGACACCTAAGGAAACACCAGAGCCGGTTGTTGAGGAGACAAAAG 10 0.25 No Hit CCTAATGTTGGGAAGAGCTCTTTCATCAACAAGATCACCAGGGCAGATGT 9 0.22499999999999998 No Hit TGGCGTTCTTTATGAAGGGAGAGCCTTTGGTGGCGAAGCTAGCAGCTATA 8 0.2 No Hit GGCTAAAGAGCTGAATTATGAGAACTTCAACAGCAGCAGCAGTATTAATA 8 0.2 No Hit GATGTCCTGTCAAAAAGTGCAGCTTCTTCCATCATTGACATTCCATTGTT 8 0.2 No Hit GGTGGTTCGGTTCCAAATTGGAGAAGTCTTTGCTCATGTTCCAAGGGATG 8 0.2 No Hit GCGGACTTTGTTCAGGTGCATCCGGGAGTATTGTAATATACATGTAGTTT 7 0.17500000000000002 No Hit GCCAAGGATGCATGCTGTCCAGTGCTTCAAGGTCTTCTTGACTTGGATGC 7 0.17500000000000002 No Hit GGAGTTTGATCAGGGAAGTTGCTGGCTTTGCACCATATGAGAAGAGGATC 7 0.17500000000000002 No Hit TGAAGATTGAGATGGATACAAAGGTGAAAATGAAGGTGGGTGGATTAAAG 7 0.17500000000000002 No Hit GTGTGATTGCTTCCCCAGCGAAGTCCATGGTATCCAAAATCTTTGATGTT 7 0.17500000000000002 No Hit TAGGAAGGCGCCTCTGCAGATCGCTCTCTGCTTTTGCTAGCGCAACAATG 7 0.17500000000000002 No Hit GTGCAACTGTGATTGTCATCGGGTTTTATTCGGTGATGTGGGGAAAAGCC 7 0.17500000000000002 No Hit ATTCAGTCTCTGTCTCACCCACACGGCGCTCTTTTATCAGCTGACGGAGG 7 0.17500000000000002 No Hit GTTTGATTCCTGGTTTCTCATCAAGGTTTGCTGCTGCTTCTTCTGAAGAT 7 0.17500000000000002 No Hit GTCGCTGGGTCTTACAGGAGGAATGAATTGGTTCGGATTCGACATGATTC 6 0.15 No Hit GGGATGGCAGCACCCTGCTCTGGCACCAGGCGTGGGTTCAACTCATGGAT 6 0.15 No Hit TACAGATCATGGCTCGCTGACAGGTTTGACTTGTGCCATGTTTAAGAGAG 6 0.15 No Hit ATAGAGTATATGGAAAGCATGTACGTGAAGCTAACACTGTCAGCACTTCG 6 0.15 No Hit GACCTGCTCTTACATGTCTTCCGGCATCAACGCTGTGCGAGGATAGAAGC 6 0.15 No Hit AATCAGGCAAACTTGTCGACTGGGATTAGCTTTGGTAAGAGTGTCGCCTT 6 0.15 No Hit CGAGCGTGAGATGGCGGAGCATGAAGGCGAGCGTGAGCGTGAACCAGTGG 6 0.15 No Hit GAGGAGAGAAAGTTTTGGATTCTCAAGAGGGAATTGATGGAGGGAGTCTA 6 0.15 No Hit CAGCAGTTGGGAAATCACAAATACTTGCTCGATTTGCTAGAAACGAGTTT 6 0.15 No Hit GCAGCTCTCAATGACTTCGATCGGTTCAAGTTGATGTTGGCTAAGATCAA 6 0.15 No Hit GAAACACGAACTTCATACTGAGCATGAGCTGATACGTACTAAACGCAAAA 6 0.15 No Hit CATCAAACGAGTCTGAGATTTTCCCCAATATGTTGAAGTTTGCAGATGCT 6 0.15 No Hit AGATAAAGATGGGGTCTGATTATTCTTGGAGAAAACCTGGAGGGGTGGCA 6 0.15 No Hit CTGGTCGTGGTATCCTTGCCATAGACGAATCAAATGCAACCTGTGGGAAG 6 0.15 No Hit GAGCTGAATGATTCTGAGAATGTTTTGGTTGCTGAAACTAAAGATGCATC 6 0.15 No Hit GAGCTCTTTCAACCAAGTTGGGCTCAAGATCACTTGGCTTATGAAGGAGA 6 0.15 No Hit ACTTCCTCTCATGGCCAGTGTAATGTCCTAGATACCATCCACTTGCACCC 6 0.15 No Hit CGAGAGGTTTCTTTTGCATCGAGCAAGAAATCTGGATTTCGAGTATTGAT 6 0.15 No Hit CTAAGGCAGAAACATGCACTTCCATCTGGTGAGGGTGCACCGTATGGAGT 6 0.15 No Hit GCTATTGGACGCCTTTGTGAGAAGTGTGATGGAAAATGTGTAATCTGCGA 5 0.125 No Hit GTGAAAGCTCAGGTCTCTGAAGTTAAGGGTGTCATGATGGAGAACATTGA 5 0.125 No Hit CTCTGGAATCAAGCCAGAAATTGCAATGACCATTCCCAAACGATGCAACA 5 0.125 No Hit TGGAGATGGCATATCTAGGATTCAGAATAAGACATCATTCGGGTTGTTCC 5 0.125 No Hit GTGGGTGCCTTTCTGCAAGAAGCACAATATTGAGCCTAGGGCCCCGGAGT 5 0.125 No Hit ATCCAGCAGCATCAAGACCCACCATGAGAATTCTCATTTCTTTCTTGGCA 5 0.125 No Hit ATATTTACATAGAAAAGGCACTAAACAGGCTCCTCCCAAATAAAAATATA 5 0.125 No Hit GGATTATCAGTATGCTTTGATTGGCCAGGCTAGCAGGAAATATCTCTGGA 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 5 0.125 No Hit GTTTATCTTGGCTTGATTCCCAACCAAGCCAAAGTGTTGTGTTTCTTTGT 5 0.125 No Hit CAGGAAGGCCCTAGAACAACGTGCTGGTGAATTTCTTCAAAATATGGGGC 5 0.125 No Hit CACCACATCCTGGTATAATGCTGCCACTAAAGCGCTCAAGGATTTGAAGT 5 0.125 No Hit TATGATGGTAACTCAACCTCAAGTTCGTATTCTTTGTGTTCTGTATGGTG 5 0.125 No Hit GTCAATTTTGGGCTTTAATGTAACTTTAGAAGTGATACTGGAGACTATTG 5 0.125 No Hit CAGTTGCCATGGCAACAAAGAAGGCTACTGAGGGTGTCCCTGGTGTTGGA 5 0.125 No Hit CTCGGGGGCAGCTTCTCGAGCCACTCCTGGGCGATGTTGACCTCGCATTC 5 0.125 No Hit GCACATAACTCACCATCAATTTACCAGGATGAGATGCCCAACCATAGATA 5 0.125 No Hit ATCAACATTGCAATGAGCATAGACCCTGAGTGGAAAATGATCAGGCCTGA 5 0.125 No Hit AAGAGAGATTTCGCAATATGCGTTTGATGGAAGAATACGATACTCATGAT 5 0.125 No Hit TGAAGGTATTGGAAATTAATGGATCAGATGCAGGTGAAAATGGGTGCAAG 5 0.125 No Hit GGTTGGAGAAGCAGGATCCTCTCGAGTATTATTGTCAAGACAATCCTGAG 5 0.125 No Hit CCAACACATTCGGGTCATTTGTGCTCCTCGCAGTTTTTGCGTTGGGTGGC 5 0.125 No Hit GTTAAAGGAGAGAGATCCTTCGCTGAAGATGTTGAGCCGAGGCCTAATGT 5 0.125 No Hit GAATGGGGGCGGAGAAAATTAACAGGGCCAGCAGCTAAACACAAAAAACC 5 0.125 No Hit GTTGAAGCAGATTGGTGGTATGGGTGGCTTGCAAAATTTGATGAAGCAAA 5 0.125 No Hit TGGGGCTGCTTTTGGTCTTGAGGATACAACAATATTGCTTGCTCTCTTCG 5 0.125 No Hit GGATGATCGGCAACGCCGTAAGAGGAAGAGTAGGAAGGAAAAGAGGAGAA 5 0.125 No Hit CATTTACCGAGGAACATCTAAAGCAATTGGAGGAGGAGCTTGAGGAAAAG 5 0.125 No Hit GCGAAAGGTTGATTGGCTAACTGCCAAGATGGTTGAATTTAATTTCACTG 5 0.125 No Hit GTAACAGAATCATTAGGAAGAGGATCCATGTTAGAGTGGAGCATTTGCTG 5 0.125 No Hit GAAGAAGATCACCATTTTCGTCATGGGCAAGTGCTACCCTACAGTGAGCG 5 0.125 No Hit AGAATAGTTCAGATTGCAGCATAGTCAGCCAGCTGGGATTCCCACGCATA 5 0.125 No Hit GTGGAATAGACAGTGACTTGCAGGCAAGGCTAGACAATCTGAGGAGAATG 5 0.125 No Hit TGACATCGTTGACACATTTAGGCTCCAGGAGCAACCTCCATTTGACAAGA 5 0.125 No Hit CTCGGCCGTCCATGGAAGCTGTACTCTAGAACTGTGTACATGTTATCATT 5 0.125 No Hit ATCTTCTCTACGAAGAAGAAATCTTGAGAAACTCGTTTTCTTTAAAGCTC 5 0.125 No Hit GTTTGTTGCCATGCCTGAACAGACACCTCCTAATCATGAGGCAGTGATCT 5 0.125 No Hit CGATACTATGAGGGCATTTCAAGTGGGCCTCGAAGTGCCCATAACTGGGC 5 0.125 No Hit GGGAAAATTGGAATAGCGGACCGAATTTAGATAAGCTGATAGATGCGACA 5 0.125 No Hit CCAATGATTAGAGGCATCGGGGGCGCAACGCCCTCGACCTATTCTCAAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0125 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.2375 0.0 0.0 0.0 0.0 86-87 0.38749999999999996 0.0 0.0 0.0 0.0 88-89 0.5625 0.0 0.0 0.0 0.0 90-91 0.625 0.0 0.0 0.0 0.0 92-93 0.7 0.0 0.0 0.0 0.0 94-95 0.8625 0.0 0.0 0.0 0.0 96-97 1.1625 0.0 0.0 0.0 0.0 98-99 1.3875000000000002 0.0 0.0 0.0 0.0 100-101 2.0875000000000004 0.0 0.0 0.0 0.0 102-103 2.3 0.0 0.0 0.0 0.0 104-105 2.5875 0.0 0.0 0.0 0.0 106-107 3.0875 0.0 0.0 0.0 0.0 108-109 3.575 0.0 0.0 0.0 0.0 110-111 3.7875 0.0 0.0 0.0 0.0 112-113 3.9749999999999996 0.0 0.0 0.0 0.0 114-115 4.25 0.0 0.0 0.0 0.0 116-117 4.574999999999999 0.0 0.0 0.0 0.0 118-119 5.05 0.0 0.0 0.0 0.0 120-121 5.9 0.0 0.0 0.0 0.0 122-123 6.7625 0.0 0.0 0.0 0.0 124-125 7.512499999999999 0.0 0.0 0.0 0.0 126-127 7.9624999999999995 0.0 0.0 0.0 0.0 128-129 8.662500000000001 0.0 0.0 0.0 0.0 130-131 9.7875 0.0 0.0 0.0 0.0 132-133 10.8125 0.0 0.0 0.0 0.0 134-135 11.3 0.0 0.0 0.0 0.0 136-137 12.05 0.0 0.0 0.0 0.0 138-139 12.7625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra Read 1987196 spots for SRR26075326.sra Written 1987196 spots for SRR26075326.sra SRR ids: ['SRR26075326.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_k425hop2 SRR26075326.sra spots: 39743920 blocks: [[1, 1987196], [1987197, 3974392], [3974393, 5961588], [5961589, 7948784], [7948785, 9935980], [9935981, 11923176], [11923177, 13910372], [13910373, 15897568], [15897569, 17884764], [17884765, 19871960], [19871961, 21859156], [21859157, 23846352], [23846353, 25833548], [25833549, 27820744], [27820745, 29807940], [29807941, 31795136], [31795137, 33782332], [33782333, 35769528], [35769529, 37756724], [37756725, 39743920]] SRR26075326 file size 14678305 SRR26075326 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075326 SRR26075326_1.fastq SRR26075326_2.fastq Input file: SRR26075326_1.fastq Paired file: SRR26075326_2.fastq trimmed: SRR26075326-trimmed-pair1.fastq, SRR26075326-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 20:18:17 2025 >> started Tue Feb 11 20:19:04 2025 >> done (47.490s) 39743920 read pairs processed; of these: 201 ( 0.00%) short read pairs filtered out after trimming by size control 175885 ( 0.44%) empty read pairs filtered out after trimming by size control 39567834 (99.56%) read pairs available; of these: 7131000 (18.02%) trimmed read pairs available after processing 32436834 (81.98%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 24 0.00% 19 15 0.00% 20 24 0.00% 21 17 0.00% 22 24 0.00% 23 25 0.00% 24 36 0.00% 25 44 0.00% 26 34 0.00% 27 25 0.00% 28 52 0.00% 29 52 0.00% 30 42 0.00% 31 57 0.00% 32 82 0.00% 33 74 0.00% 34 55 0.00% 35 41 0.00% 36 68 0.00% 37 82 0.00% 38 73 0.00% 39 77 0.00% 40 83 0.00% 41 117 0.00% 42 102 0.00% 43 107 0.00% 44 103 0.00% 45 132 0.00% 46 127 0.00% 47 158 0.00% 48 229 0.00% 49 194 0.00% 50 215 0.00% 51 235 0.00% 52 292 0.00% 53 353 0.00% 54 351 0.00% 55 462 0.00% 56 466 0.00% 57 501 0.00% 58 622 0.00% 59 687 0.00% 60 843 0.00% 61 918 0.00% 62 1036 0.00% 63 1257 0.00% 64 1397 0.00% 65 1425 0.00% 66 1639 0.00% 67 1863 0.00% 68 2070 0.01% 69 2497 0.01% 70 2960 0.01% 71 3159 0.01% 72 3880 0.01% 73 4471 0.01% 74 5057 0.01% 75 5895 0.01% 76 6649 0.02% 77 7232 0.02% 78 7932 0.02% 79 9089 0.02% 80 10060 0.03% 81 11127 0.03% 82 13342 0.03% 83 14337 0.04% 84 16890 0.04% 85 18222 0.05% 86 19388 0.05% 87 21902 0.06% 88 23886 0.06% 89 25022 0.06% 90 27041 0.07% 91 29432 0.07% 92 32083 0.08% 93 34726 0.09% 94 38257 0.10% 95 41662 0.11% 96 43983 0.11% 97 46786 0.12% 98 49249 0.12% 99 52221 0.13% 100 54130 0.14% 101 56543 0.14% 102 60518 0.15% 103 63288 0.16% 104 66619 0.17% 105 70125 0.18% 106 74317 0.19% 107 76997 0.19% 108 80059 0.20% 109 81929 0.21% 110 84197 0.21% 111 86429 0.22% 112 89219 0.23% 113 92564 0.23% 114 95685 0.24% 115 100402 0.25% 116 104882 0.27% 117 106751 0.27% 118 110546 0.28% 119 112301 0.28% 120 114974 0.29% 121 117433 0.30% 122 119043 0.30% 123 121609 0.31% 124 126061 0.32% 125 129785 0.33% 126 133297 0.34% 127 137161 0.35% 128 140680 0.36% 129 143565 0.36% 130 144801 0.37% 131 146911 0.37% 132 148037 0.37% 133 151140 0.38% 134 153588 0.39% 135 155914 0.39% 136 157997 0.40% 137 160860 0.41% 138 164086 0.41% 139 167922 0.42% 140 170377 0.43% 141 170109 0.43% 142 175150 0.44% 143 174380 0.44% 144 179206 0.45% 145 179838 0.45% 146 181064 0.46% 147 184484 0.47% 148 187486 0.47% 149 187704 0.47% 150 191393 0.48% 151 32436834 81.98% 39567834 reads passed initial QC criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=3.46 fanout-score-rank=22 prefix-density=0.50 prefix-fanout=2.1 sequence=CACTTGCAGCCATTCTCAGCACC criterion=fanout-score sequence-density=0.04 sequence-density-rank=28 fanout-score=89.58 fanout-score-rank=1 prefix-density=0.36 prefix-fanout=9.1 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.39 sequence-density-rank=1 fanout-score=2.90 fanout-score-rank=26 prefix-density=0.40 prefix-fanout=2.8 sequence=ATGTACCCTGACTTAGGTTTCTCAGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=70.68 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=3.0 sequence=TCCTGCTCTCGCAATCGCTGCTTCTTTGTCTGTCTTTGGGTCGATCCGAAAGAGAGGAGCTCTTCTGCGCAATCATGTTGGTCTATCAAGATCTTCTCTCTGGTGATGAGCTTCTCTCGGATTCGTTCCCATACAAGGAGATTGAGAATGGGATACTGTGGGAAGTTGAAGGAAAGTGGGTTGTTCAAGGAGCCGTTGATGTAGACATTGGTGCAAATCCTTCAGCTGAAGGAGGTGATGAGGATGAGGGTGTTGATGACCAAGCTGCCAAGGTTGTTGACATCGTTGACACATTTAGGCTCCAGGAGCAACCTCCATTTGACAAGAAGCAGTTTCTTACACAGATTAAGAAATTTATCAAGAATCTGTCGGAGAAACTTGATGAGGACCAGAAGGAACATTTTAGAAAGAACATTGAGGGAGCAACCAAGTTCTTGCTTTCAAAAATCAAGGACTTGCAATTCTTTGTGGGGGAGAGCATGCATGATGATGGTTGTTTGGTCTTTGCTTA SRR26075326 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 20:19:45 Started mapping on | Feb 11 20:19:45 Finished on | Feb 11 20:25:43 Mapping speed, Million of reads per hour | 397.89 Number of input reads | 39567834 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 35393856 Uniquely mapped reads % | 89.45% Average mapped length | 291.45 Number of splices: Total | 32009620 Number of splices: Annotated (sjdb) | 31231086 Number of splices: GT/AG | 31446854 Number of splices: GC/AG | 429914 Number of splices: AT/AC | 29945 Number of splices: Non-canonical | 102907 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 3.09 Insertion rate per base | 0.02% Insertion average length | 2.63 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1246273 % of reads mapped to multiple loci | 3.15% Number of reads mapped to too many loci | 197754 % of reads mapped to too many loci | 0.50% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.53% % of reads unmapped: other | 0.37% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2927705 2927705 2927705 N_multimapping 1246273 1246273 1246273 N_noFeature 905550 34962207 1125021 N_ambiguous 412868 2538 199104 UnstrandedReadsAssigned:34075438 PositiveStrandReadsAssigned:429111 NegativeStrandReadsAssigned:34069731 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075326 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075326-trimmed-pair1.fastq SRR26075326-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 39,567,834 reads, 34,714,518 reads pseudoaligned [quant] estimated average fragment length: 206.953 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,262 rounds 52401 SRR26075326.ke.tsv 34699 SRR26075326.se.tsv 87100 total ==> SRR26075326.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1812.05 3891 48.9054 Potri.005G024800.1.v4.1 1035 829.047 2193 60.2455 Potri.004G059700.1.v4.1 961 755.047 13 0.392134 Potri.007G009000.2.v4.1 1416 1210.05 0 0 Potri.003G141000.2.v4.1 2943 2737.05 1317 10.9589 Potri.016G087400.1.v4.1 270 92.5167 3085.59 759.598 Potri.015G069301.1.v4.1 564 359.105 0 0 Potri.010G195200.1.v4.1 1773 1567.05 834 12.1213 Potri.012G127500.1.v4.1 977 771.047 50107 1480.07 ==> SRR26075326.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 366 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 620 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1505 SRR26075326 completed mapping pipeline successfully