Starting /dee2/code/volunteer_pipeline.sh SRR26075327 current disk space = 3053446324224 free memory = 1431688804 SRR26075327 SRAfilesize b12c94471a2c4d75164aeaa781649326 SRR26075327.sra SRR26075327.sra file validated SRR26075327 is paired end SRR26075327 is conventional basespace SRR26075327 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075327_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.64375 37.0 37.0 37.0 37.0 37.0 2 36.585 37.0 37.0 37.0 37.0 37.0 3 36.6395 37.0 37.0 37.0 37.0 37.0 4 36.611 37.0 37.0 37.0 37.0 37.0 5 36.679 37.0 37.0 37.0 37.0 37.0 6 36.7035 37.0 37.0 37.0 37.0 37.0 7 36.637 37.0 37.0 37.0 37.0 37.0 8 36.618 37.0 37.0 37.0 37.0 37.0 9 36.663 37.0 37.0 37.0 37.0 37.0 10-14 36.64020000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.601 37.0 37.0 37.0 37.0 37.0 20-24 36.591300000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.4953 37.0 37.0 37.0 37.0 37.0 30-34 36.471399999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.433800000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.3391 37.0 37.0 37.0 37.0 37.0 45-49 36.2505 37.0 37.0 37.0 37.0 37.0 50-54 36.1255 37.0 37.0 37.0 37.0 37.0 55-59 36.0404 37.0 37.0 37.0 37.0 37.0 60-64 36.0261 37.0 37.0 37.0 37.0 37.0 65-69 36.0492 37.0 37.0 37.0 37.0 37.0 70-74 36.0654 37.0 37.0 37.0 37.0 37.0 75-79 36.0855 37.0 37.0 37.0 37.0 37.0 80-84 36.0338 37.0 37.0 37.0 37.0 37.0 85-89 35.9707 37.0 37.0 37.0 37.0 37.0 90-94 35.9151 37.0 37.0 37.0 37.0 37.0 95-99 35.9992 37.0 37.0 37.0 37.0 37.0 100-104 35.8406 37.0 37.0 37.0 37.0 37.0 105-109 35.9264 37.0 37.0 37.0 37.0 37.0 110-114 35.743399999999994 37.0 37.0 37.0 37.0 37.0 115-119 35.5854 37.0 37.0 37.0 37.0 37.0 120-124 35.693900000000006 37.0 37.0 37.0 37.0 37.0 125-129 35.562599999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.4088 37.0 37.0 37.0 37.0 37.0 135-139 35.3988 37.0 37.0 37.0 34.6 37.0 140-144 35.186099999999996 37.0 37.0 37.0 29.8 37.0 145-149 35.2144 37.0 37.0 37.0 27.4 37.0 150-151 35.076499999999996 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 0.0 18 0.0 19 2.0 20 1.0 21 2.0 22 5.0 23 5.0 24 6.0 25 4.0 26 7.0 27 6.0 28 14.0 29 15.0 30 26.0 31 38.0 32 72.0 33 114.0 34 166.0 35 438.0 36 2906.0 37 172.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.58268701526144 15.761821366024517 6.980235176382286 33.675256442331744 2 19.55 14.149999999999999 34.65 31.65 3 17.95 18.475 29.425 34.150000000000006 4 21.825 24.875 24.825 28.475 5 23.25 31.900000000000002 24.5 20.349999999999998 6 20.5 35.65 24.025 19.825 7 16.175 28.775000000000002 39.7 15.35 8 17.974999999999998 28.7 31.15 22.175 9 18.425 22.55 34.125 24.9 10-14 20.979999999999997 29.725 26.834999999999997 22.46 15-19 20.605 27.800000000000004 27.565 24.03 20-24 21.575 27.96 26.895000000000003 23.57 25-29 21.78 28.439999999999998 27.48 22.3 30-34 20.105 28.970000000000002 27.229999999999997 23.695 35-39 20.580000000000002 27.96 27.900000000000002 23.56 40-44 20.07 28.050000000000004 27.794999999999998 24.085 45-49 20.51 28.715000000000003 26.3 24.474999999999998 50-54 20.855 27.089999999999996 28.15 23.905 55-59 21.13 27.694999999999997 27.42 23.755000000000003 60-64 20.635 28.075 28.03 23.26 65-69 20.84 27.85 27.389999999999997 23.919999999999998 70-74 21.57 27.515 27.665 23.25 75-79 20.905 27.589999999999996 27.965 23.54 80-84 20.635 28.025 27.555000000000003 23.785 85-89 21.715 27.944999999999997 26.27 24.07 90-94 20.7 27.485 27.105 24.709999999999997 95-99 20.979999999999997 28.29 27.655 23.075000000000003 100-104 20.23 27.095000000000002 29.075 23.599999999999998 105-109 20.765 28.804999999999996 27.139999999999997 23.29 110-114 20.435 28.050000000000004 26.834999999999997 24.68 115-119 21.415 27.935 27.12 23.53 120-124 21.475 28.395 26.884999999999998 23.244999999999997 125-129 22.285 27.92 26.605 23.189999999999998 130-134 21.65 27.250000000000004 26.96 24.14 135-139 22.295 27.77 26.06 23.875 140-144 22.495 27.655 26.775 23.075000000000003 145-149 22.56 27.250000000000004 26.505000000000003 23.685000000000002 150-151 22.2125 28.65 24.337500000000002 24.8 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.0 4 0.5 5 0.0 6 0.5 7 1.5 8 2.0 9 1.5 10 1.0 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.5 19 1.5 20 0.5 21 0.5 22 3.0 23 2.5 24 2.0 25 3.0 26 4.5 27 9.5 28 7.0 29 7.0 30 18.0 31 28.0 32 32.0 33 33.5 34 50.5 35 62.0 36 73.0 37 91.0 38 108.5 39 133.0 40 166.5 41 222.0 42 231.0 43 239.5 44 259.5 45 248.5 46 261.0 47 266.5 48 236.0 49 207.5 50 184.0 51 150.5 52 133.0 53 122.0 54 92.0 55 68.0 56 53.0 57 33.0 58 20.0 59 17.5 60 15.5 61 10.0 62 11.0 63 11.0 64 8.5 65 7.0 66 6.0 67 3.0 68 2.0 69 4.0 70 5.0 71 6.0 72 7.0 73 5.5 74 2.0 75 1.0 76 1.5 77 1.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.12500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 61.01479915433404 36.075 2 22.494714587737842 26.6 3 8.921775898520085 15.825 4 4.397463002114165 10.4 5 1.5644820295983086 4.625 6 0.9302325581395349 3.3000000000000003 7 0.3382663847780127 1.4000000000000001 8 0.2536997885835095 1.2 9 0.042283298097251586 0.22499999999999998 >10 0.042283298097251586 0.35000000000000003 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGACAACAAAAGCTGCTCCTCCGAAATAACAGTCCCCCATCCCACGCCC 14 0.35000000000000003 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAATCTCGGTT 9 0.22499999999999998 TruSeq Adapter, Index 25 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAATCTCGGGT 8 0.2 TruSeq Adapter, Index 25 (97% over 38bp) ACCCAACTCAAAGACTTTATCAGCTGCAAAGGCTGCCAAGTCAGTGTTCC 8 0.2 No Hit ATTGATATCTGCTGGCTGAGGAGCTGCAGCGGGTTTGGAAGGAGGTGGAT 8 0.2 No Hit CTTGCAAGGGCCACACCATTGGGTGTACATGTCGAGGACGACAGTCTTAT 8 0.2 No Hit CTTCGATATGCGGGTCAAGCTTGCGAGTCTGTTGTCTCTTCTCCAGCTTT 8 0.2 No Hit GTAAAATTCTGAGCCAAAATTAAGGAGGGAACCCTCGACCTCTATCAATA 8 0.2 No Hit AGAAGATTTGCAGCAGTGGAGATCGCAGCTCCAGTGATAGCACATTGCAC 7 0.17500000000000002 No Hit ACCACTCTTAATAATATGGGTAAAGGAAACTGCAACCTTCGACATGCTCA 7 0.17500000000000002 No Hit GTAGAGGATTGATACCGATGATGATGCTGATGCTGCTGCTGCTGTAAATC 7 0.17500000000000002 No Hit CTTGTCTCAATCTCAGCTGTAGGGTTTCTCAAACTCATCTGCATGGACTG 7 0.17500000000000002 No Hit CTGTGACTTGCCGGTTTGCCAGGCATGCATTAACCGAAGACTCTGCCAGC 7 0.17500000000000002 No Hit ACCGGAATGCTCAACAGCTTCTTGCTCCAGCAAGTTCACTGCTTCTTCCA 7 0.17500000000000002 No Hit CCCAGGATGAGTCCCTCAGCGTGACGCAGTAAACTAGTAGCATCCCGATC 7 0.17500000000000002 No Hit CTGCAGGACCAGATAGTAACATGCGTGGGGACACAGTAGGGAGATCGCAA 7 0.17500000000000002 No Hit GCATGGTTGAAGTTGAGTTTGGCACACTGATCGTGTGGCCATGTGCCAAA 6 0.15 No Hit AAGGTATTCTTCCTCCGTTGAAGGGGACTCACTACGAGATCTCTTGGAGC 6 0.15 No Hit AACCATTATAAAATTCATCAGCCTACTTGGAACCAACAACAACACCCCAT 6 0.15 No Hit GGCAGAGTGGGGGCATTGAAGGGACATGCACACCATTGACATTGGTATTC 6 0.15 No Hit ATGAATTTTAAGCACCTCTCTGGCCTTGTCTTTGGTCTTGCTTCCGCAAT 6 0.15 No Hit CATCAGTGAGGGTGGGAGGGCCCGTGTTCATGTAATAGCCTCACGCCTTC 6 0.15 No Hit GGATGATTTTGATGAAGTTTCCTGGGGGGCTGCTGCTGAATCGATGCTCA 6 0.15 No Hit CCCAAAAAAAGTGAAGGTAGATCATCTTCAGCATTTGAAGCCACCACAAA 6 0.15 No Hit GTCAAATTTAAAAACCGACCCCCAAGAGACAGGCTAAAGATAACATGATC 6 0.15 No Hit ATCCCAGACCCATCTCCCCCGGGAAAATCATGCCAGAGATGTTTGGATTC 6 0.15 No Hit GGGCGGCACTGTGACATCTTGAGGAGCTACAAGCATTGCTCGACCACCAT 6 0.15 No Hit GGACTTTGCTTAATTGAAGAGGCATAAAGGGAAAGAGATTGGACGCACAA 6 0.15 No Hit GTTACAGAGATGGCAGATACAAGAGGTCCATTCAGAGCTGGTGGGTTATA 6 0.15 No Hit ATCATCACCGGATACAAAGAAATCTGAAGAGAACTCTGGCAAGGCTGGAA 6 0.15 No Hit CCCTGCCATACAAGACCCAAACCCAAAACCAAGAAACCTACACCAATTAT 6 0.15 No Hit ACCCATAAGTCTTCAGCAAACAGTCAAATGTTGCCTTGACGAAGTTCCCA 6 0.15 No Hit ACAATTTTGATTCAATGACCATTATTTATGTCAATCTTAAGGAAAGTTGA 6 0.15 No Hit GTGATTACCAGAGAAGGTGGGAGGAAGTGTCATGTGGTGGACTTGTTCTA 6 0.15 No Hit CTCCAAAAATCATCTGATATGCTAGGTTTCGTCACCACTCGCCCTTGTAT 6 0.15 No Hit CTACATAACATGCAAAAAAACTCAAACTAGACGAATTGTCCTCGTATGTG 6 0.15 No Hit TTTTTTTTTTGAGGGGTCATAAAAGTGCCGTGAAGACTGTTTGGACTACT 6 0.15 No Hit GGCAAGAGGTGTGAAGCGGCGTTCGGGGAGGTTTTCCATTGGCGAAAACT 6 0.15 No Hit AGGATTTATAATACAGATATGCCACCCTAAAAAGTCAGAGTTAGAGCATC 5 0.125 No Hit TGGAAATCTGATCACCGGAAATATAAGATTTGCCGACGAGAAACTCGTTG 5 0.125 No Hit GTTCATTTGAATTCCACCATGAAGGGCAACTGACAGTAAAGTGTGGTTCA 5 0.125 No Hit ATAATAAATACAGTAACTTGAAAAACCTATCCAGAAAACCCATCAGTTTT 5 0.125 No Hit TACGATAGCCAGTCTCGCTGCATCGATAGTTTTCATCACTCTTCTCAGAG 5 0.125 No Hit CTCAACAGACTTGACTTCAGTACTCAGTCCAGTTGGACCGAATGTGACAA 5 0.125 No Hit CGGAGATCGGTGGACAGTGGGGCGCTCATTAGGATCCTGCGCAAGGAGGA 5 0.125 No Hit CATGCATGCATCACTAGCCAGATTAGGAGTTAATTATATTTTACTAATAA 5 0.125 No Hit CGTGGGGCTGGCTGGCAGGTGCCATATTTCAGAGTACAGTTTGGAATCAC 5 0.125 No Hit GCCCGATCCTTGTCCTTCTTACTCAATGACAAAATGTCACTCTCCACAGC 5 0.125 No Hit CATTCATCAATCTATAACTCTTCGTAAGCAAAAGCGTAATTACATGTACA 5 0.125 No Hit ATCCAATCCTGTATCAAGCTCTTACTATTTTGGTGATAAGCCGCCAGCTT 5 0.125 No Hit GGGCGAAAGACATGTATCGGTAATTGTCCATCTTAAACTCTTGGCTAAAA 5 0.125 No Hit CTCGAAAACATCATCCAAGAGTAGTTTTGGAACACTTGCCCTGATCACAT 5 0.125 No Hit GTTTGATAATGGTCACAGCCTTCAGTTTCTTTCCGTTGTTTCTGCCGTGC 5 0.125 No Hit GTGAGCTATAGAATTTCCTTTGACAATAGTTGCACGAAAACACTCGAGGC 5 0.125 No Hit CCTGGATAACGCGGCGACAGTACACAATTCACTGAAGAATGCTTGAAAAA 5 0.125 No Hit GTTGTACTTATCTGACCAATGCGCAGCTGCTGGAACAGCCACCCCTGCAA 5 0.125 No Hit GCCAGCAATTTCTCGCCTTTTCCCTTCATTCAGGACAAAATTGCTTTGAG 5 0.125 No Hit ACCACATCGACACTTAAACCCTGTTAAACCAACTCTTTTCTTGCAAGAGG 5 0.125 No Hit GCACCGTGTCTGAACTTCGTATTTACTCAATTCTAAGAACAGCAATCTCT 5 0.125 No Hit ACTTGTGGTGGCAGGAAGCCCTGACCTAACATCTACTTCTCTAAGACTCC 5 0.125 No Hit CCTCAGGCACGAAATCCATGCGACGCTCACGCTCTTCCTCTTGTAGTTTC 5 0.125 No Hit GTCTGTTTCCGACTCCTTCACATTTCGGAAACCATTACAATGAGAACTCA 5 0.125 No Hit GCTGATGTTTCCTCTTCTTGGGCTGGCTTTTTCTCCTCCTCGGCTACCAC 5 0.125 No Hit CCTGCTGACTCTGAAATGGTGTGAACTAATAATGTTGTTATAATCAAGCC 5 0.125 No Hit CTCCGAACTTTCTAAGACACAACACGACCAACAACAGGGTACTTAGCCTC 5 0.125 No Hit GATCAAACGGTACTGCGTCTCTCCACAACAAGAGATCATGAAGAAGAGGG 5 0.125 No Hit GTCTACAATGGTAAGCATGATTTGTAGCTTCTTGATTCCATAACCGACTG 5 0.125 No Hit CTAGGCTAAGCTACCTGAATTGGCAGAGCATCCATATCAGCTCGAACAGC 5 0.125 No Hit CCTCATCATCATCAGCATCATGATCTTCTTTTGGATTCTTATTTCGATCG 5 0.125 No Hit CAACAATATGCGAGGAGGGCAACCAAATTTACTGCTCAGCATGGCTGTCA 5 0.125 No Hit ACCTGCTAGTTGCTAAAATGCGCCTGTTGATCTCCGTGTCCATCCCAAAC 5 0.125 No Hit ATTAGCTTCTTTAATCTTTCCTCAAACTCTCCACGGTATTTAGTTCCAGC 5 0.125 No Hit GCTAGTTAAAACGAGGTGTATAAAGCTATGATAAGAAACTTGATGATCAA 5 0.125 No Hit ACCAGTTTACCTTTCTCCGATCCGAATTTCTTCCAGTTCCAAACTCCTGT 5 0.125 No Hit GTTTTTTGACTAGAAGATTTCAACAGGAACTTGATGATCCCATGAGAAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0875 0.0 0.0 0.0 0.0 82-83 0.1 0.0 0.0 0.0 0.0 84-85 0.1 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.1 0.0 0.0 0.0 0.0 90-91 0.1 0.0 0.0 0.0 0.0 92-93 0.1125 0.0 0.0 0.0 0.0 94-95 0.1375 0.0 0.0 0.0 0.0 96-97 0.1875 0.0 0.0 0.0 0.0 98-99 0.325 0.0 0.0 0.0 0.0 100-101 0.325 0.0 0.0 0.0 0.0 102-103 0.325 0.0 0.0 0.0 0.0 104-105 0.375 0.0 0.0 0.0 0.0 106-107 0.5625 0.0 0.0 0.0 0.0 108-109 0.675 0.0 0.0 0.0 0.0 110-111 0.7124999999999999 0.0 0.0 0.0 0.0 112-113 0.85 0.0 0.0 0.0 0.0 114-115 1.0125 0.0 0.0 0.0 0.0 116-117 1.15 0.0 0.0 0.0 0.0 118-119 1.375 0.0 0.0 0.0 0.0 120-121 1.6375 0.0 0.0 0.0 0.0 122-123 2.15 0.0 0.0 0.0 0.0 124-125 2.45 0.0 0.0 0.0 0.0 126-127 2.9749999999999996 0.0 0.0 0.0 0.0 128-129 3.3375000000000004 0.0 0.0 0.0 0.0 130-131 4.0 0.0 0.0 0.0 0.0 132-133 4.4125 0.0 0.0 0.0 0.0 134-135 4.85 0.0 0.0 0.0 0.0 136-137 5.3875 0.0 0.0 0.0 0.0 138-139 6.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACACG 10 0.006830828 145.0 145 >>END_MODULE SRR26075327 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075327_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.307 37.0 37.0 37.0 37.0 37.0 2 36.2775 37.0 37.0 37.0 37.0 37.0 3 36.124 37.0 37.0 37.0 37.0 37.0 4 36.2235 37.0 37.0 37.0 37.0 37.0 5 36.191 37.0 37.0 37.0 37.0 37.0 6 36.2065 37.0 37.0 37.0 37.0 37.0 7 36.0985 37.0 37.0 37.0 37.0 37.0 8 36.1355 37.0 37.0 37.0 37.0 37.0 9 36.2405 37.0 37.0 37.0 37.0 37.0 10-14 36.089299999999994 37.0 37.0 37.0 37.0 37.0 15-19 35.96040000000001 37.0 37.0 37.0 37.0 37.0 20-24 35.9414 37.0 37.0 37.0 37.0 37.0 25-29 35.812200000000004 37.0 37.0 37.0 37.0 37.0 30-34 35.6678 37.0 37.0 37.0 37.0 37.0 35-39 35.5383 37.0 37.0 37.0 37.0 37.0 40-44 35.502599999999994 37.0 37.0 37.0 37.0 37.0 45-49 35.382099999999994 37.0 37.0 37.0 37.0 37.0 50-54 35.21660000000001 37.0 37.0 37.0 37.0 37.0 55-59 35.2556 37.0 37.0 37.0 37.0 37.0 60-64 35.3303 37.0 37.0 37.0 37.0 37.0 65-69 35.2308 37.0 37.0 37.0 37.0 37.0 70-74 35.129 37.0 37.0 37.0 37.0 37.0 75-79 35.0961 37.0 37.0 37.0 34.6 37.0 80-84 35.1031 37.0 37.0 37.0 37.0 37.0 85-89 35.1109 37.0 37.0 37.0 32.2 37.0 90-94 35.153800000000004 37.0 37.0 37.0 32.2 37.0 95-99 35.1209 37.0 37.0 37.0 34.6 37.0 100-104 35.07379999999999 37.0 37.0 37.0 32.2 37.0 105-109 35.0602 37.0 37.0 37.0 29.8 37.0 110-114 34.9654 37.0 37.0 37.0 25.0 37.0 115-119 34.9694 37.0 37.0 37.0 27.4 37.0 120-124 34.8889 37.0 37.0 37.0 27.4 37.0 125-129 34.8788 37.0 37.0 37.0 25.0 37.0 130-134 34.8059 37.0 37.0 37.0 25.0 37.0 135-139 34.7205 37.0 37.0 37.0 25.0 37.0 140-144 34.7251 37.0 37.0 37.0 25.0 37.0 145-149 34.7494 37.0 37.0 37.0 25.0 37.0 150-151 34.4865 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 8.0 14 13.0 15 16.0 16 15.0 17 9.0 18 12.0 19 8.0 20 13.0 21 13.0 22 15.0 23 19.0 24 13.0 25 22.0 26 16.0 27 20.0 28 14.0 29 21.0 30 21.0 31 33.0 32 33.0 33 96.0 34 195.0 35 621.0 36 2528.0 37 225.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.925000000000004 22.0 9.45 19.625 2 29.049999999999997 27.825 25.95 17.175 3 24.175 29.549999999999997 30.15 16.125 4 27.224999999999998 32.574999999999996 20.674999999999997 19.525000000000002 5 26.0 36.3 20.8 16.900000000000002 6 24.3 35.9 21.25 18.55 7 22.95 22.975 34.65 19.425 8 25.324999999999996 24.675 26.674999999999997 23.325000000000003 9 25.25 25.55 25.55 23.65 10-14 26.445 28.749999999999996 24.51 20.294999999999998 15-19 26.150000000000002 27.33 25.990000000000002 20.53 20-24 25.650000000000002 28.025 26.07 20.255000000000003 25-29 25.56 27.939999999999998 25.785000000000004 20.715 30-34 26.290000000000003 28.115000000000002 25.685000000000002 19.91 35-39 25.785000000000004 28.410000000000004 25.759999999999998 20.044999999999998 40-44 25.669999999999998 27.935 25.945 20.45 45-49 25.88 27.345000000000002 26.765 20.01 50-54 23.565 27.900000000000002 27.944999999999997 20.59 55-59 25.169999999999998 27.68 26.939999999999998 20.21 60-64 25.09 27.965 26.88 20.064999999999998 65-69 26.465 28.050000000000004 25.845000000000002 19.64 70-74 24.645 28.849999999999998 26.419999999999998 20.085 75-79 24.445 28.76 26.1 20.695 80-84 25.009999999999998 26.945000000000004 27.175 20.87 85-89 25.474999999999998 27.875 26.51 20.14 90-94 25.45 28.715000000000003 26.08 19.755 95-99 24.125 29.270000000000003 26.525 20.080000000000002 100-104 25.590000000000003 28.88 25.785000000000004 19.744999999999997 105-109 24.51 28.77 26.66 20.06 110-114 25.069999999999997 27.900000000000002 26.419999999999998 20.61 115-119 25.205 28.994999999999997 26.179999999999996 19.62 120-124 25.095 28.175 27.339999999999996 19.39 125-129 25.319999999999997 28.15 26.895000000000003 19.634999999999998 130-134 25.295 28.860000000000003 26.090000000000003 19.755 135-139 26.43 28.975 24.89 19.705000000000002 140-144 26.279999999999998 28.62 26.090000000000003 19.009999999999998 145-149 26.655 27.91 25.775 19.66 150-151 26.775 27.787499999999998 26.137500000000003 19.3 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.5 11 1.0 12 1.0 13 1.5 14 1.5 15 0.5 16 1.5 17 2.5 18 2.0 19 3.5 20 4.5 21 2.5 22 2.0 23 3.0 24 3.0 25 4.0 26 5.0 27 4.0 28 3.0 29 6.0 30 13.5 31 15.0 32 18.5 33 21.0 34 30.5 35 52.5 36 68.5 37 84.5 38 93.0 39 134.5 40 199.5 41 222.5 42 231.0 43 250.5 44 271.0 45 294.5 46 293.0 47 246.0 48 204.0 49 206.0 50 194.0 51 155.5 52 118.5 53 92.0 54 76.5 55 60.5 56 43.5 57 23.5 58 24.0 59 32.5 60 22.0 61 12.0 62 9.0 63 10.0 64 9.0 65 3.0 66 2.0 67 1.5 68 2.5 69 2.0 70 2.5 71 2.0 72 0.5 73 1.5 74 1.0 75 1.0 76 1.5 77 2.5 78 2.5 79 2.0 80 2.5 81 2.0 82 2.5 83 4.0 84 4.0 85 2.5 86 2.5 87 3.5 88 5.0 89 7.5 90 6.0 91 2.5 92 2.0 93 1.5 94 3.0 95 4.5 96 3.5 97 1.5 98 1.0 99 1.5 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.275 #Duplication Level Percentage of deduplicated Percentage of total 1 63.83243467440896 38.475 2 21.236001659062627 25.6 3 8.129406885109914 14.7 4 3.815844048112816 9.2 5 1.534632932393198 4.625 6 0.7880547490667772 2.85 7 0.3732890916632103 1.575 8 0.16590626296142677 0.8 9 0.0 0.0 >10 0.1244296972210701 2.175 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 48 1.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 25 0.625 No Hit GGGAGGTGCTGATTGCCGTCCGATCCAGCCTGATGCAACGTGTTATAGTC 14 0.35000000000000003 No Hit GCTTGGACACGGCGGGTCCCACTTCCGCTGTTGGACAGGTCACTGAGGTC 8 0.2 No Hit AGCTGCCAAGAAGGAAGGAGAGGATGGTGAGGCTGCTGTTGAGGAGGAGA 8 0.2 No Hit TGTTGAGCATGGGATGTTTCTTGATATGGCAACCACTGTTATTGTTGCAG 8 0.2 No Hit GAAATATTTTGCACTCATGAAGAAGTATGAGATTGAGCCAGGGACCCAAC 8 0.2 No Hit CGTTCAAAGGGTTTAAATTTCCTTCTGTAAAGTTTAGATATGGGTCGTGG 7 0.17500000000000002 No Hit CTGGGACAGGTCAGCCATGAATTAAATTGTGAATATCTCAGTGGTTTGAC 7 0.17500000000000002 No Hit GGGATCCCTCACCCTCCTCCAAAATTCAACAGTTTGCCTGTGCTTAGCCA 7 0.17500000000000002 No Hit CAGAAGCCTATCAAATCGATCAGGCCACCTTTAATCCCTACCGAGGATGA 7 0.17500000000000002 No Hit GCGAGGCTTAATGAGGCTGCTGTAAAGAAGAAAAAGGAAATTGCTGATTT 7 0.17500000000000002 No Hit CTTCTCAGCGGAGCAGAAAGATCATGTTTATTGGAAGCATCAACCTGCGC 7 0.17500000000000002 No Hit GCTCAATCCACTTCACACAATGTCGAGTATCAATTTGGCAGTACTGCTGG 7 0.17500000000000002 No Hit GATGTCCACTAGACGGCAATTGTTCAAGGATAGTTTACAAAAAGGAATTC 7 0.17500000000000002 No Hit GGAAAGGAATCCTTACAATGGGGACTCGCAGCAGGAGTGTATTCCGGCCT 7 0.17500000000000002 No Hit GACAACCAATCTGGCGGTTCCAATCTACCCATAATATTCAGGTCCGCAAA 6 0.15 No Hit TAGCTCAATTCCTGCTTGGATCGTTCACTTTCTTGCTTGTATGGGTGGCT 6 0.15 No Hit CGAAACCAAAACCAGCCAAACCAGCCACTCCTGAAACTCCAGCAACTCCA 6 0.15 No Hit GGGGATTAAAGATTAGTTACTATGGCCTTTGGGGTCTGATGGGAAGACGA 6 0.15 No Hit CAAAGATTTGGTTTTTGTGATGAATTATGATACGGGGTTTCATCCTCTAT 6 0.15 No Hit GTTGACCTCCACTCTTTCTCTTGCTTGCGGATCTCTTATGATGATGATCT 6 0.15 No Hit CAAGATCGGAAAGCCACATACTGTGCCTTGTAAGGTTACAGGGAAGTGTG 6 0.15 No Hit GTTGTAGTTACTGTTCTCACCCTTCAGGTCATAGTAGTACTTCCCTTTTT 6 0.15 No Hit ACTTCACTTTGCTGAAACTGTTTATACGCAGGATGAAGACTATAGCAGCT 6 0.15 No Hit TTTCTTGGGAGTTCTTCGTCAAAGAACTGACCCCGGGTTTACTGATCAGC 6 0.15 No Hit ATTGAAATGGGCAGACAGAGAGAACACCGTCCGTAACTCCTCTCTCTTTT 6 0.15 No Hit GAGGAAGATTCACGAGAATCCTGAGCTTGCTTTTGAAGAAGTCAAAACAA 6 0.15 No Hit CTCTCTCAATCTCAATACCTTCCCTTCTCTCTTCGGAAAAACTTATTTGT 6 0.15 No Hit GCAAGCACGGCAAACTGTGCTAGCACCACCACAAAGTCAAGTTCAAGGGC 6 0.15 No Hit GAAGGGAGTCCTGCGGGAATCAAGAAACTCAAAGGAAGCCCAAAGAAATG 6 0.15 No Hit AAGAAAGGGAGAGAAGAGAAGAGAAGATATCTTTGTGGTTATGATATCAG 6 0.15 No Hit GGTGGATCCGCCGGTTGGGCACCGCCATATGCCGGGTACGGTCAACAACA 6 0.15 No Hit GAAAAGTTGATTGCCATACTTGATCTGCAGCAAATTGCATATAAGAACAT 6 0.15 No Hit GCAAAGAATACAGTACTAATGGTTCATGTTCATCATCCTCTTCTCATTTG 6 0.15 No Hit AAGGAATCATCTATTGATTGGAAACTCATTTCTGATATTGATGGCAGCTA 5 0.125 No Hit CGTTCACTCAGAAACTTCAGGTCATTACAGAGCTTTCCTTCTCGCTCTCT 5 0.125 No Hit GTAATTATTCCGGAAGAGGGAGATTCTCATCTGCAGATAGTCGTCATGTT 5 0.125 No Hit GTTGCAAAAGATTGATAAATCTACTGGTGGGTTGAGCAATCTAGATGCAA 5 0.125 No Hit GTGAGAGTTCTGTTGCTGGTTTGGGATGATAAGACTTCTCATAACAAGTT 5 0.125 No Hit CATCCCCTCGAAGCGTCTCCGCAACAAGATTGCTGGGTTCTCTACCCATC 5 0.125 No Hit GGTGAGCCAAGCACACTATTTGATTGAGAAAGCTTCAGAAAAGGCGAAAG 5 0.125 No Hit GTTCATAATTGAAGATCTCGACTTGTTTGTGCAAAGAAAGGACTACTTTC 5 0.125 No Hit AAAACAGAAAACCTTCGCTCTCAGGCACAAGTTTTCAGGCAGCAAGGGGG 5 0.125 No Hit CGCCACCTCTCCCAAAAAACCCCTAACCCCCGCAGCTGCAGTAGCCACAA 5 0.125 No Hit GGAAATGGGGACCACCACCACCACCACCTGGGGTGGATTCCGACCACCGC 5 0.125 No Hit CAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATAC 5 0.125 No Hit GCTGTGTAAAAGTGGACCAATCCACGGTTGTTATCAAGGAGAGATTTGGA 5 0.125 No Hit AAACGGCTTGCAAGTCTTTCATGGTAGTTCGTTCTCGAGAACCAGTAATG 5 0.125 No Hit GTACCACCAGATGAGGTGCCTTTTCCATCTTCACTTCAGGAGACTATCGA 5 0.125 No Hit TCACTTTCGATTGCTCTCCTTCTGGTCCCTGCGTTCCCTGCGCCTACTCT 5 0.125 No Hit CGGCGCCACCACCACCACCAAAGTCGGAGGCGGCGCCACCACCACCTGCC 5 0.125 No Hit AGCTGTTGGTGTAAGCATTTGTGGCAAAACAGCTGCTGCTGCTCCCGTTG 5 0.125 No Hit GGATCATGAATTGGAGCTGTAACTTTCTGTAAAACAACCCTAATAATGTA 5 0.125 No Hit GCGGACTTGCCCTGGAAAGACATTGGGCATAACCACGGTGACCTTCTGGG 5 0.125 No Hit GAAAAGATGCCTCAGTTGGTTTCTTTTAAGGAGGAAAGTACTAAAGTTGC 5 0.125 No Hit GAATAATTTTTACAATGCATACGGAGGGAAACAGTTTCCATTCTATTACC 5 0.125 No Hit AAGCAGGCGAAGAAAACCAATCAATGTTGTACCATACTGTAGCTGCAATG 5 0.125 No Hit GCAGACTAAGCTTGCTGCATCATCAATTGGAAGTATTGTGAATGGATCGG 5 0.125 No Hit GGTGGAATGGGTGGAATGGGCGGTATGGATATGGATATGGGTAATGTGGA 5 0.125 No Hit TGGAGTCCAAGCTAAGCATGCTACATATGTCCCACACACAGCTGGGAGAT 5 0.125 No Hit CATTGCGGGCAGAAGGTTCCATAGCGGGACACTAAACGGTTCTTCTATTG 5 0.125 No Hit CTTTGACATACCAAACCCCTTTTAAGCTATCAATCTTCTTGATATTTCAA 5 0.125 No Hit ACAAGATTCCCTTTGTCCCAATCTCTGGATTTGAGGGTGACAACATGATT 5 0.125 No Hit GTTTGTATCTGCCATTATAAAGAAGTTTCCTCCAGCAACTCCTTTCTTAA 5 0.125 No Hit GGTGCATTAAGGATTTGAAAGAGAGGTTTGCAAAAGTGAGCCTGGAAGTC 5 0.125 No Hit GAAATCCTCTCTTCTTATTCTTCGCATGGAGCCTCCTGCTAGATCAGAGG 5 0.125 No Hit ACAAGAGGTGTCACTACGAAGGCATCATAGGAGGCGCCGAAAAGAGTGGT 5 0.125 No Hit CAAGAAGATGTCAAGTACTAACTTCATAGACATCTTGCTGGCCATCATCT 5 0.125 No Hit GTTCAGATGCACACAAAGCTGCTGTCATTGGTGACACAATTGGAGATCCC 5 0.125 No Hit ATAAAGAGCGTTTGTTTTTTGAAGGAAAAAGAGAGAGAAAATGAATGAAA 5 0.125 No Hit CAGGTTGTGTAAAGAAGTGGATTGCGGAAAAGGAACATGCGTAGGAGACA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0875 0.0 0.0 0.0 0.0 82-83 0.1 0.0 0.0 0.0 0.0 84-85 0.1 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.1 0.0 0.0 0.0 0.0 90-91 0.1 0.0 0.0 0.0 0.0 92-93 0.1125 0.0 0.0 0.0 0.0 94-95 0.1375 0.0 0.0 0.0 0.0 96-97 0.25 0.0 0.0 0.0 0.0 98-99 0.425 0.0 0.0 0.0 0.0 100-101 0.425 0.0 0.0 0.0 0.0 102-103 0.425 0.0 0.0 0.0 0.0 104-105 0.48750000000000004 0.0 0.0 0.0 0.0 106-107 0.6875 0.0 0.0 0.0 0.0 108-109 0.8 0.0 0.0 0.0 0.0 110-111 0.8374999999999999 0.0 0.0 0.0 0.0 112-113 1.025 0.0 0.0 0.0 0.0 114-115 1.2125 0.0 0.0 0.0 0.0 116-117 1.35 0.0 0.0 0.0 0.0 118-119 1.5750000000000002 0.0 0.0 0.0 0.0 120-121 1.8125 0.0 0.0 0.0 0.0 122-123 2.325 0.0 0.0 0.0 0.0 124-125 2.625 0.0 0.0 0.0 0.0 126-127 3.175 0.0 0.0 0.0 0.0 128-129 3.5625 0.0 0.0 0.0 0.0 130-131 4.2125 0.0 0.0 0.0 0.0 132-133 4.6125 0.0 0.0 0.0 0.0 134-135 5.025 0.0 0.0 0.0 0.0 136-137 5.5625 0.0 0.0 0.0 0.0 138-139 6.1875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTTTG 10 0.006830828 145.0 5 ATATTTT 10 0.006830828 145.0 4 TGTACGG 10 0.006830828 145.0 145 >>END_MODULE Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra Read 1179605 spots for SRR26075327.sra Written 1179605 spots for SRR26075327.sra Read 1179599 spots for SRR26075327.sra Written 1179599 spots for SRR26075327.sra SRR ids: ['SRR26075327.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_skg66mef SRR26075327.sra spots: 23591986 blocks: [[1, 1179599], [1179600, 2359198], [2359199, 3538797], [3538798, 4718396], [4718397, 5897995], [5897996, 7077594], [7077595, 8257193], [8257194, 9436792], [9436793, 10616391], [10616392, 11795990], [11795991, 12975589], [12975590, 14155188], [14155189, 15334787], [15334788, 16514386], [16514387, 17693985], [17693986, 18873584], [18873585, 20053183], [20053184, 21232782], [21232783, 22412381], [22412382, 23591986]] SRR26075327 file size 8708629 SRR26075327 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075327 SRR26075327_1.fastq SRR26075327_2.fastq Input file: SRR26075327_1.fastq Paired file: SRR26075327_2.fastq trimmed: SRR26075327-trimmed-pair1.fastq, SRR26075327-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 19:16:23 2025 >> started Tue Feb 11 19:16:51 2025 >> done (27.298s) 23591986 read pairs processed; of these: 122 ( 0.00%) short read pairs filtered out after trimming by size control 159559 ( 0.68%) empty read pairs filtered out after trimming by size control 23432305 (99.32%) read pairs available; of these: 2537153 (10.83%) trimmed read pairs available after processing 20895152 (89.17%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 11 0.00% 20 12 0.00% 21 12 0.00% 22 7 0.00% 23 24 0.00% 24 24 0.00% 25 17 0.00% 26 22 0.00% 27 24 0.00% 28 28 0.00% 29 40 0.00% 30 31 0.00% 31 36 0.00% 32 39 0.00% 33 49 0.00% 34 22 0.00% 35 35 0.00% 36 38 0.00% 37 30 0.00% 38 38 0.00% 39 63 0.00% 40 31 0.00% 41 50 0.00% 42 50 0.00% 43 45 0.00% 44 55 0.00% 45 62 0.00% 46 58 0.00% 47 76 0.00% 48 87 0.00% 49 84 0.00% 50 86 0.00% 51 103 0.00% 52 112 0.00% 53 114 0.00% 54 127 0.00% 55 112 0.00% 56 124 0.00% 57 151 0.00% 58 143 0.00% 59 163 0.00% 60 174 0.00% 61 219 0.00% 62 223 0.00% 63 250 0.00% 64 282 0.00% 65 298 0.00% 66 253 0.00% 67 323 0.00% 68 333 0.00% 69 364 0.00% 70 436 0.00% 71 455 0.00% 72 484 0.00% 73 551 0.00% 74 665 0.00% 75 752 0.00% 76 734 0.00% 77 763 0.00% 78 828 0.00% 79 966 0.00% 80 1076 0.00% 81 1206 0.01% 82 1212 0.01% 83 1326 0.01% 84 1653 0.01% 85 1779 0.01% 86 1950 0.01% 87 2044 0.01% 88 2287 0.01% 89 2408 0.01% 90 2559 0.01% 91 2986 0.01% 92 3287 0.01% 93 3800 0.02% 94 4207 0.02% 95 4580 0.02% 96 5001 0.02% 97 5547 0.02% 98 5918 0.03% 99 6483 0.03% 100 6824 0.03% 101 7697 0.03% 102 8730 0.04% 103 9418 0.04% 104 10666 0.05% 105 11114 0.05% 106 12474 0.05% 107 13551 0.06% 108 14043 0.06% 109 15352 0.07% 110 15732 0.07% 111 17297 0.07% 112 19144 0.08% 113 20590 0.09% 114 22388 0.10% 115 25191 0.11% 116 26108 0.11% 117 28241 0.12% 118 29511 0.13% 119 30677 0.13% 120 32320 0.14% 121 34493 0.15% 122 36789 0.16% 123 39318 0.17% 124 43051 0.18% 125 45444 0.19% 126 47654 0.20% 127 49564 0.21% 128 52748 0.23% 129 54703 0.23% 130 55460 0.24% 131 58016 0.25% 132 60413 0.26% 133 62790 0.27% 134 66905 0.29% 135 69853 0.30% 136 72108 0.31% 137 74451 0.32% 138 76162 0.33% 139 79283 0.34% 140 80977 0.35% 141 81848 0.35% 142 84170 0.36% 143 86248 0.37% 144 90815 0.39% 145 92083 0.39% 146 95263 0.41% 147 96937 0.41% 148 98999 0.42% 149 98876 0.42% 150 101564 0.43% 151 20895152 89.17% 23432305 reads passed initial QC criterion=sequence-density sequence-density=0.17 sequence-density-rank=1 fanout-score=14.14 fanout-score-rank=5 prefix-density=0.11 prefix-fanout=14.1 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACACGGAACAATCTCGT criterion=fanout-score sequence-density=0.03 sequence-density-rank=40 fanout-score=93.39 fanout-score-rank=1 prefix-density=0.33 prefix-fanout=7.7 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=2.51 fanout-score-rank=37 prefix-density=0.35 prefix-fanout=2.5 sequence=ATGTACCCTGACTTAGGTTTCTCAGAGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=46 fanout-score=243.09 fanout-score-rank=1 prefix-density=0.16 prefix-fanout=11.2 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTA SRR26075327 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 19:17:53 Started mapping on | Feb 11 19:17:56 Finished on | Feb 11 19:23:21 Mapping speed, Million of reads per hour | 259.56 Number of input reads | 23432305 Average input read length | 298 UNIQUE READS: Uniquely mapped reads number | 19878644 Uniquely mapped reads % | 84.83% Average mapped length | 296.43 Number of splices: Total | 18400913 Number of splices: Annotated (sjdb) | 17956752 Number of splices: GT/AG | 18058155 Number of splices: GC/AG | 263284 Number of splices: AT/AC | 21268 Number of splices: Non-canonical | 58206 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 2.98 Insertion rate per base | 0.02% Insertion average length | 2.65 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 520287 % of reads mapped to multiple loci | 2.22% Number of reads mapped to too many loci | 82496 % of reads mapped to too many loci | 0.35% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 11.88% % of reads unmapped: other | 0.72% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3033374 3033374 3033374 N_multimapping 520287 520287 520287 N_noFeature 542505 19636077 695914 N_ambiguous 213952 1317 123872 UnstrandedReadsAssigned:19122187 PositiveStrandReadsAssigned:241250 NegativeStrandReadsAssigned:19058858 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075327 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075327-trimmed-pair1.fastq SRR26075327-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 23,432,305 reads, 19,506,136 reads pseudoaligned [quant] estimated average fragment length: 221.117 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,265 rounds 52401 SRR26075327.ke.tsv 34699 SRR26075327.se.tsv 87100 total ==> SRR26075327.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1797.88 2388 57.1633 Potri.005G024800.1.v4.1 1035 814.883 1799 95.0124 Potri.004G059700.1.v4.1 961 740.895 8 0.464705 Potri.007G009000.2.v4.1 1416 1195.88 0 0 Potri.003G141000.2.v4.1 2943 2722.88 746.594 11.8005 Potri.016G087400.1.v4.1 270 82.5571 1849.58 964.193 Potri.015G069301.1.v4.1 564 345.781 0 0 Potri.010G195200.1.v4.1 1773 1552.88 563 15.6032 Potri.012G127500.1.v4.1 977 756.895 8591 488.486 ==> SRR26075327.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 150 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 281 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1085 SRR26075327 completed mapping pipeline successfully