Starting /dee2/code/volunteer_pipeline.sh SRR26075328 current disk space = 3053307494400 free memory = 1465981728 SRR26075328 SRAfilesize c88387ab383a3981469fcb56814a0a88 SRR26075328.sra SRR26075328.sra file validated SRR26075328 is paired end SRR26075328 is conventional basespace SRR26075328 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075328_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5655 37.0 37.0 37.0 37.0 37.0 2 36.5315 37.0 37.0 37.0 37.0 37.0 3 36.616 37.0 37.0 37.0 37.0 37.0 4 36.686 37.0 37.0 37.0 37.0 37.0 5 36.7095 37.0 37.0 37.0 37.0 37.0 6 36.69 37.0 37.0 37.0 37.0 37.0 7 36.66 37.0 37.0 37.0 37.0 37.0 8 36.6505 37.0 37.0 37.0 37.0 37.0 9 36.6015 37.0 37.0 37.0 37.0 37.0 10-14 36.61665 37.0 37.0 37.0 37.0 37.0 15-19 36.63290000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.53950000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.480199999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.383500000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.3234 37.0 37.0 37.0 37.0 37.0 40-44 36.3037 37.0 37.0 37.0 37.0 37.0 45-49 36.2718 37.0 37.0 37.0 37.0 37.0 50-54 36.2509 37.0 37.0 37.0 37.0 37.0 55-59 36.1777 37.0 37.0 37.0 37.0 37.0 60-64 36.169200000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.0747 37.0 37.0 37.0 37.0 37.0 70-74 36.077 37.0 37.0 37.0 37.0 37.0 75-79 36.0694 37.0 37.0 37.0 37.0 37.0 80-84 36.0616 37.0 37.0 37.0 37.0 37.0 85-89 35.9293 37.0 37.0 37.0 37.0 37.0 90-94 35.9137 37.0 37.0 37.0 37.0 37.0 95-99 35.931799999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.8602 37.0 37.0 37.0 37.0 37.0 105-109 35.8071 37.0 37.0 37.0 37.0 37.0 110-114 35.756600000000006 37.0 37.0 37.0 37.0 37.0 115-119 35.6322 37.0 37.0 37.0 37.0 37.0 120-124 35.7117 37.0 37.0 37.0 37.0 37.0 125-129 35.5774 37.0 37.0 37.0 37.0 37.0 130-134 35.453 37.0 37.0 37.0 37.0 37.0 135-139 35.375899999999994 37.0 37.0 37.0 34.6 37.0 140-144 35.2457 37.0 37.0 37.0 29.8 37.0 145-149 35.367599999999996 37.0 37.0 37.0 37.0 37.0 150-151 35.20225 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 3.0 22 1.0 23 3.0 24 2.0 25 9.0 26 6.0 27 8.0 28 17.0 29 26.0 30 31.0 31 40.0 32 57.0 33 119.0 34 155.0 35 437.0 36 2860.0 37 224.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.4360902255639 11.077694235588972 6.466165413533835 28.020050125313283 2 25.6 12.925 31.525 29.95 3 22.325 17.775 30.15 29.75 4 26.525 25.8 22.45 25.224999999999998 5 26.525 31.0 22.575 19.900000000000002 6 24.474999999999998 32.7 23.575 19.25 7 21.475 25.25 36.675000000000004 16.6 8 22.775000000000002 22.825 28.825 25.575 9 20.474999999999998 22.775000000000002 30.975 25.775 10-14 24.7912395619781 26.466323316165806 24.616230811540575 24.126206310315514 15-19 25.119999999999997 25.555 25.735000000000003 23.59 20-24 24.315 25.545 25.945 24.195 25-29 24.375 26.33 25.240000000000002 24.055 30-34 23.325000000000003 25.825 25.545 25.305 35-39 24.575 25.34 25.97 24.115000000000002 40-44 24.145 26.66 25.11 24.085 45-49 23.87 25.724999999999998 25.955000000000002 24.45 50-54 23.995 25.124999999999996 25.569999999999997 25.31 55-59 24.34 25.35 25.35 24.959999999999997 60-64 24.525 25.495 25.509999999999998 24.47 65-69 24.125 26.064999999999998 25.174999999999997 24.635 70-74 24.755 26.405 25.169999999999998 23.669999999999998 75-79 24.905 25.369999999999997 25.03 24.695 80-84 24.82 25.045 25.330000000000002 24.805 85-89 24.2 25.995 25.545 24.26 90-94 25.074999999999996 24.89 25.94 24.095 95-99 25.275 24.959999999999997 25.805 23.96 100-104 24.060000000000002 25.509999999999998 25.165 25.264999999999997 105-109 24.83 25.435000000000002 25.135 24.6 110-114 26.13 25.34 24.82 23.71 115-119 25.069999999999997 25.080000000000002 25.46 24.39 120-124 25.695 26.35 24.065 23.89 125-129 24.605 26.450000000000003 25.135 23.810000000000002 130-134 25.615 26.26 24.38 23.745 135-139 24.22 25.290000000000003 24.815 25.674999999999997 140-144 25.495 26.235000000000003 23.865 24.404999999999998 145-149 24.975 25.805 23.865 25.355 150-151 25.074999999999996 25.7375 25.2375 23.95 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.0 22 0.5 23 0.0 24 0.5 25 0.5 26 2.5 27 8.5 28 9.5 29 6.0 30 5.0 31 8.5 32 16.5 33 21.5 34 25.5 35 33.0 36 40.5 37 54.5 38 79.5 39 85.0 40 96.0 41 119.5 42 143.5 43 182.5 44 214.0 45 203.5 46 173.5 47 168.5 48 174.5 49 188.0 50 190.5 51 150.5 52 137.0 53 150.0 54 152.0 55 148.5 56 152.0 57 139.5 58 99.0 59 104.0 60 102.5 61 79.5 62 70.5 63 67.5 64 54.5 65 41.5 66 38.0 67 25.5 68 15.0 69 7.0 70 1.0 71 2.0 72 2.0 73 1.5 74 3.5 75 2.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.3 #Duplication Level Percentage of deduplicated Percentage of total 1 65.12841091492777 40.575 2 20.34510433386838 25.35 3 8.467094703049758 15.825 4 3.410914927768861 8.5 5 1.4044943820224718 4.375 6 0.6019261637239166 2.25 7 0.32102728731942215 1.4000000000000001 8 0.20064205457463882 1.0 9 0.08025682182985554 0.44999999999999996 >10 0.04012841091492777 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTAAACAAACAGAAGTATTATGCCAACAATGTTGGATATGCCATTAGCT 11 0.27499999999999997 No Hit GACAATACTTGATAATTGTTCTAGCGACAGCTTTTTTAGATTAGGAAGCA 9 0.22499999999999998 No Hit CGGGCAGGCATTTATCTGAATAGCATTTTCTTAACTTCATATCCTTGAGC 9 0.22499999999999998 No Hit CCAGGGAACCCAAGATCCACGGGCTTCGCACCGTCGAGGTAAGCGATCTG 8 0.2 No Hit GGTTTACTTGTGGCTGTGCAGGCTGTGGTGCCACTCTAGAGACATCTTGC 8 0.2 No Hit GTCCTCTCCAAATCCTTCCTCTTCACAATAAAGCCCATCAAGAACCAATG 8 0.2 No Hit GTGGCTTTAAATCCCGATGGCAAACTCCAGGAACAGCATGGATATAAGCC 8 0.2 No Hit TTTCGATACGGGACGTTGCCGTGTTGAATAAAGAGCGGGTCCCTCACTAG 8 0.2 No Hit GTCATACTTGAAATGTCATGATCCTAGTAGATGCGCTCTGGCGCTTCTTG 7 0.17500000000000002 No Hit TAGAAGAAACTATCCAATACCAAAATTGAACGCATGCATAAAAATAGAGA 7 0.17500000000000002 No Hit GGCGCATCGGCTGGTGCCACCACAGATCCATTCCACAGGGTGTTGTCATA 7 0.17500000000000002 No Hit GTACATAACAACAGATGGGAAAGTTATATCTCATCTTGCCTAAGCAAGTT 7 0.17500000000000002 No Hit CGGAGATGGTGCTCTGCAGTCCGTTGATGGTGACCCGAAGCTCCTCGGCT 7 0.17500000000000002 No Hit GTTTCGAAGAAAGATTCCAGGATTTCGTCTGCAAGGACGACCATCCGGAA 7 0.17500000000000002 No Hit TTCTTCTTTGCGTCTTCCCGCTGTGAAGGCTCCTTGAAGGTGTCGTTGGA 7 0.17500000000000002 No Hit GGGATCGGGGTCGGGACACGGCTGGATAGCCACAAGACAGCCGCCGCTCC 7 0.17500000000000002 No Hit CCTTGGAGAGACTTAGAAGAGATAGTAGTCCATGATCATACGGCTTGCAC 6 0.15 No Hit GGTGGTCGATGGAAATCACGTCCAGAGTGGGCAGCTCTGGTCCAACATCA 6 0.15 No Hit GCTGGTGCATCGTCGGCGGCATCATCGTCTGCGCCTCCTTGCGCCTCAGC 6 0.15 No Hit ATGTAAACTAGAGAGGAGTACGTGGCTAAACTAGTTAGGAAGTGAACTCC 6 0.15 No Hit GTGAAATCTTGGATCAGTTCATCGCGGGCATCGAGGTATTCATCTTCGAA 6 0.15 No Hit GTTGGCAGAGAGAGCGAGAGATAATGCGTGTGCAAGACAGCGGACAGTGC 6 0.15 No Hit GGGATTCAATGTCCGATTCAAGCATCTCTACAACTTTATGCATTGAGGGA 6 0.15 No Hit ATAAAAACAAACAACGCATGGGACAAAATCCTATTGTAAACAAGTCTCAA 6 0.15 No Hit GTTGTAGTTGTCAGCGTTGTTGGCAGCAGATGCAGGGTCAGATGAAGCCA 6 0.15 No Hit GAGGAGCTGGTGTAGAGGGTTGAGGAGCTGGAGTAGAGGGTTGAGGAGTA 6 0.15 No Hit CTATATTCCCGATCTCTCTCTTCACACTTCTTCTGAGACCACTTCTCTAG 6 0.15 No Hit GTCTTGGTGTTGGCCTTTTCCAACGAGAGCGAGAGCTCACGAGGAACATC 6 0.15 No Hit ACTCTCTCATCTTCCGGGTCCAAAACCGGGTATTTCTCAATCAACTCCTC 6 0.15 No Hit GCTTGCCCATACTTTGCGAGATGCGGTTCAGGACTCAAAGGGAGTGCTCG 6 0.15 No Hit GCTGGGAGGCGACGGAGTGGGTAATGGGGACTCCGTAACTCGGGGCTGCA 6 0.15 No Hit GGGCTTCTTGGGTTCGGGAGGTGGGGGTGAGGGCTTGCGTCCAAGGGCAC 5 0.125 No Hit GATGCAGCAGCAGAAAGTGCGGGAACGAACGGCGGCTTGGTTGTGAGGAG 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCGGTTATCGCGTAT 5 0.125 TruSeq Adapter, Index 18 (97% over 38bp) GCTTCAGCCTAAGCCAATGTGAAGTAGAAATCTCAAAACTCTGCAAAGTG 5 0.125 No Hit CTCTAGAACACCGTACTCGATGGACTTGGTTTGGCTGTCATCACCGAAGC 5 0.125 No Hit GCCCATTGCATAAGGTTGGTAAGCCACCATATCTCCCTTACTCACGTTAA 5 0.125 No Hit CCTCAGACCACTTGGTGGTGTCCATCTTGTTGACGGCGACAATGAGCTGA 5 0.125 No Hit GTGTGGTGTAGGATGTTAAAGTGTCTTTCAGAGATCAACTCTTGAAGGGG 5 0.125 No Hit GTCATTCTTGGAGGTGGATCTAATGGCCGATCATCATCCTTATTAGTTTC 5 0.125 No Hit GTTCTTTATCATCAATGTGGTTCGGTTGTCATCGCCACGCAGTATGCAGT 5 0.125 No Hit CTTGGGCCTCCGAGCAGGTCTCTGGCACAACATCACCATGAAATCCTCTT 5 0.125 No Hit GGAAGGAAGGTGCTTCGCGGCCTCCTCGACGCTCCAGCTCTCCAGCACGA 5 0.125 No Hit GGCCTAATTCAGGGTCCTGCACCACTGTACCATTACAGCAGAACTCTTTC 5 0.125 No Hit GAAACAGATTCCAAACTTTCATGCCCATCCAATCTACAATTCGTCAGTAT 5 0.125 No Hit CAGGTGGAGGCGGCACAGGTAAACTTGGATTGCCAGACATTAATGGAGGC 5 0.125 No Hit GTTCTTGGTGGGGCAGGGGGTCGGGGCAGCAGTCACAGTGCGAGAGATGG 5 0.125 No Hit GCATGATCCTGTTCTTCTCGATGGAGATGCCGTTTTCCTCCAAGTCAGCA 5 0.125 No Hit GTAAACTGGGTTGTACTTGGAAGATTGGACTATGAAACAAAACCCATTAA 5 0.125 No Hit GGCAAAAGCAACAAATATTCTCCTATGAAGACAAATGAACTTTTTGTTAC 5 0.125 No Hit GTGGCTTTATAGGAGGCTTTGGTGGCTTTATTGGAGGAGTCACAGGAGGC 5 0.125 No Hit TTCATTGTGGATGGTTGTCTCCATGTAAGTCTCTGCAGAGGCGCCAATTC 5 0.125 No Hit GGCGCAATTGCTTGTGCATGTGGGTGCAGGGGTAAACAGGAAGAGCATCT 5 0.125 No Hit GTGAGGATCAACGAAGGGTTGGTTGTAGCATCCTGGGGCTTGTAGGTATC 5 0.125 No Hit CCAACATTTGCCCTGATTCAATCAATAGGCATACAAGTTCACGCCCTCGT 5 0.125 No Hit GTGGAGATAAGGAAGCAGGAATAACAGAATTTGTATTAGACAAATCCATA 5 0.125 No Hit CTCTTACCTCATCGGCAATATTTATTGTACGATTGGATGCATTTGGCTGG 5 0.125 No Hit GGGGTGGTGACCTTCTTCACCTTCTTGTCCTTGGTAGAGGCGGTAGTGGC 5 0.125 No Hit CCCGGGCGACCTTGTTTGCTTTCGCGTGGCCCTGGATGGCAGTCTTTCCT 5 0.125 No Hit ATTTTTGCAATATCAATAGCAGGTAACAACTCAAAAATCTTGAGCTTCAG 5 0.125 No Hit GGAGTAGTGGCATTCAGGCCGTGAATCGTCGCGGAAACGTTGAACAACGC 5 0.125 No Hit CCGTCATGAACACATTCAAATGCTGAGATCATGGCTTGGACATTTCCTGT 5 0.125 No Hit GCCTGATTATCCTCTAAAACAATTAAATCCTCCCCCTGAACAATTAACTT 5 0.125 No Hit CTATAAACATCTGATTTCAGAGTTAGTTGCCCAGTCATTGCATATTCTGG 5 0.125 No Hit CGGGGTGCTCCTCAGGGGCGACACGCAGCTCGTTGTAGAAGGTGTGATGC 5 0.125 No Hit GTGATTCTGAAAACAAGCCAGCCTTCTCTCTAATTGTCTTCATGACCTTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.21250000000000002 0.0 0.0 0.0 0.0 88-89 0.3 0.0 0.0 0.0 0.0 90-91 0.35 0.0 0.0 0.0 0.0 92-93 0.4875 0.0 0.0 0.0 0.0 94-95 0.5375000000000001 0.0 0.0 0.0 0.0 96-97 0.575 0.0 0.0 0.0 0.0 98-99 0.6125 0.0 0.0 0.0 0.0 100-101 0.7 0.0 0.0 0.0 0.0 102-103 0.8 0.0 0.0 0.0 0.0 104-105 0.8875 0.0 0.0 0.0 0.0 106-107 0.9874999999999999 0.0 0.0 0.0 0.0 108-109 1.2000000000000002 0.0 0.0 0.0 0.0 110-111 1.7875 0.0 0.0 0.0 0.0 112-113 2.3 0.0 0.0 0.0 0.0 114-115 2.825 0.0 0.0 0.0 0.0 116-117 3.325 0.0 0.0 0.0 0.0 118-119 3.8875 0.0 0.0 0.0 0.0 120-121 4.3375 0.0 0.0 0.0 0.0 122-123 5.1 0.0 0.0 0.0 0.0 124-125 5.8625 0.0 0.0 0.0 0.0 126-127 6.4 0.0 0.0 0.0 0.0 128-129 7.775 0.0 0.0 0.0 0.0 130-131 8.4625 0.0 0.0 0.0 0.0 132-133 9.7 0.0 0.0 0.0 0.0 134-135 10.95 0.0 0.0 0.0 0.0 136-137 11.7375 0.0 0.0 0.0 0.0 138-139 12.712499999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCAAC 10 0.006830828 145.0 1 >>END_MODULE SRR26075328 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075328_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.867 37.0 37.0 37.0 37.0 37.0 2 36.16 37.0 37.0 37.0 37.0 37.0 3 36.169 37.0 37.0 37.0 37.0 37.0 4 36.044 37.0 37.0 37.0 37.0 37.0 5 36.083 37.0 37.0 37.0 37.0 37.0 6 36.0395 37.0 37.0 37.0 37.0 37.0 7 36.083 37.0 37.0 37.0 37.0 37.0 8 36.024 37.0 37.0 37.0 37.0 37.0 9 36.0715 37.0 37.0 37.0 37.0 37.0 10-14 36.0034 37.0 37.0 37.0 37.0 37.0 15-19 35.8745 37.0 37.0 37.0 37.0 37.0 20-24 35.7808 37.0 37.0 37.0 37.0 37.0 25-29 35.70870000000001 37.0 37.0 37.0 37.0 37.0 30-34 35.63 37.0 37.0 37.0 37.0 37.0 35-39 35.5824 37.0 37.0 37.0 37.0 37.0 40-44 35.534099999999995 37.0 37.0 37.0 37.0 37.0 45-49 35.483700000000006 37.0 37.0 37.0 37.0 37.0 50-54 35.3046 37.0 37.0 37.0 37.0 37.0 55-59 35.3446 37.0 37.0 37.0 37.0 37.0 60-64 35.3557 37.0 37.0 37.0 37.0 37.0 65-69 35.280699999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.183 37.0 37.0 37.0 34.6 37.0 75-79 35.1885 37.0 37.0 37.0 37.0 37.0 80-84 35.1896 37.0 37.0 37.0 37.0 37.0 85-89 35.170899999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.1148 37.0 37.0 37.0 34.6 37.0 95-99 35.1398 37.0 37.0 37.0 34.6 37.0 100-104 35.0999 37.0 37.0 37.0 29.8 37.0 105-109 34.9489 37.0 37.0 37.0 29.8 37.0 110-114 34.9095 37.0 37.0 37.0 25.0 37.0 115-119 34.8858 37.0 37.0 37.0 25.0 37.0 120-124 34.8275 37.0 37.0 37.0 25.0 37.0 125-129 34.759100000000004 37.0 37.0 37.0 25.0 37.0 130-134 34.6557 37.0 37.0 37.0 25.0 37.0 135-139 34.4995 37.0 37.0 37.0 25.0 37.0 140-144 34.4979 37.0 37.0 37.0 25.0 37.0 145-149 34.3851 37.0 37.0 37.0 25.0 37.0 150-151 34.148250000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 14.0 14 17.0 15 17.0 16 14.0 17 12.0 18 10.0 19 11.0 20 6.0 21 14.0 22 9.0 23 13.0 24 14.0 25 14.0 26 11.0 27 20.0 28 14.0 29 27.0 30 23.0 31 30.0 32 50.0 33 93.0 34 206.0 35 714.0 36 2448.0 37 198.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.175000000000004 19.625 8.025 22.175 2 30.775000000000002 19.7 26.35 23.175 3 25.6 24.025 27.35 23.025000000000002 4 28.65 29.65 21.099999999999998 20.599999999999998 5 28.275 34.050000000000004 18.8 18.875 6 24.8 34.9 19.0 21.3 7 25.1 20.825 31.025000000000002 23.05 8 23.849999999999998 23.200000000000003 22.95 30.0 9 23.75 23.125 25.15 27.975 10-14 26.69 26.035000000000004 22.525000000000002 24.75 15-19 26.529999999999998 25.55 23.72 24.2 20-24 25.590000000000003 25.6 23.845 24.965 25-29 26.135 27.165 22.8 23.9 30-34 25.319999999999997 25.805 23.825 25.05 35-39 25.47 26.265 23.905 24.36 40-44 25.624999999999996 26.0 24.16 24.215 45-49 25.645 25.735000000000003 23.97 24.65 50-54 24.605 25.905 24.535 24.955 55-59 25.365 26.97 23.955000000000002 23.71 60-64 25.95 25.669999999999998 23.474999999999998 24.905 65-69 25.72 25.61 24.255 24.415 70-74 24.87 26.090000000000003 24.595 24.445 75-79 25.069999999999997 26.640000000000004 23.66 24.63 80-84 24.755 26.96 24.635 23.65 85-89 24.8 26.229999999999997 24.445 24.525 90-94 24.740000000000002 27.075 24.665 23.52 95-99 25.735000000000003 26.479999999999997 23.775 24.01 100-104 25.205 26.605 24.415 23.775 105-109 25.06 27.345000000000002 23.830000000000002 23.765 110-114 24.815 26.875 24.07 24.240000000000002 115-119 25.095 27.275 24.635 22.994999999999997 120-124 25.44 26.87 23.555 24.135 125-129 26.095000000000002 26.845000000000002 24.055 23.005 130-134 26.490000000000002 26.115 24.075 23.32 135-139 27.6 25.895000000000003 24.295 22.21 140-144 27.6 26.76 23.205000000000002 22.435 145-149 27.605 26.19 24.67 21.535 150-151 28.775000000000002 27.287499999999998 22.8625 21.075 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 1.0 10 1.0 11 1.0 12 0.5 13 1.5 14 1.0 15 1.0 16 2.0 17 3.0 18 2.5 19 1.5 20 2.0 21 1.0 22 0.5 23 0.5 24 0.5 25 1.0 26 0.5 27 0.0 28 2.5 29 3.5 30 3.5 31 10.5 32 14.5 33 15.5 34 25.0 35 33.0 36 38.5 37 55.0 38 66.0 39 71.5 40 90.0 41 128.5 42 154.0 43 158.0 44 156.0 45 175.0 46 192.0 47 179.5 48 188.5 49 198.0 50 169.0 51 161.5 52 160.5 53 133.0 54 150.5 55 161.0 56 144.0 57 124.0 58 111.5 59 127.0 60 111.5 61 77.5 62 66.0 63 57.0 64 45.0 65 38.5 66 31.5 67 23.0 68 15.5 69 9.0 70 6.5 71 8.5 72 7.0 73 1.0 74 1.0 75 2.5 76 3.0 77 3.5 78 2.5 79 2.0 80 3.0 81 4.0 82 3.0 83 1.0 84 1.0 85 1.5 86 2.0 87 1.5 88 2.0 89 1.5 90 0.0 91 1.0 92 1.5 93 1.0 94 0.5 95 2.5 96 3.0 97 0.5 98 1.0 99 3.5 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.324999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 67.50888274772996 42.75 2 19.30517173312278 24.45 3 7.461508093170154 14.174999999999999 4 3.158310303987367 8.0 5 1.342281879194631 4.25 6 0.5921831819976312 2.25 7 0.23687327279905251 1.05 8 0.19739439399921044 1.0 9 0.07895775759968417 0.44999999999999996 >10 0.11843663639952626 1.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 43 1.075 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 11 0.27499999999999997 No Hit TGGATTGGTGTGGTCATATGTTTAGTCATTGCATGTTATTTGCTCCAAGA 11 0.27499999999999997 No Hit TGAATTGAAGCATATTATCAGAGAAGAGGATGGTGAAAGGGAAATAATTC 9 0.22499999999999998 No Hit ATCGGAGAAAGGTAAACTGGTATTTGGAGATTTCAATTATCCAGAGAATG 9 0.22499999999999998 No Hit GTTTCTTTTCCACGACAAATAACAATGAACTCTTTCTTAACTTGGTCATG 8 0.2 No Hit CAATTACCAACTCCAGCAGCTCCGATCCAACACCCTCAGAACCAATATCT 8 0.2 No Hit GAGAAAGATATGCAATATATACAAAGAGAAGCTTGAGAGATTTTACCTTC 8 0.2 No Hit GTCTTCCCCATCGTCGGCGGTCGCAAGCCCGAACAGCTCCTCGCGAACGT 8 0.2 No Hit GCCAGCCTTCGCTTCCATGGTCATCGTTGCCACCCCTACGCGACGAATCT 8 0.2 No Hit TGTTAGTCGAGGTCCATATCTTGCAGACAAGATTTATCGTGAAGCACAAC 7 0.17500000000000002 No Hit GGAGCAGCCCCTGGCAGGGGCGTTGTACCACCTGTCAGGAGGTAATCGGC 7 0.17500000000000002 No Hit CTCTACCTGTTCAAGGCTGTATTCTAGTCATTCCTTCTCTATGTCCCTGT 7 0.17500000000000002 No Hit GTCAGTCGATTCATGTCAAATGCATTCGAGTATGGTGATGCGCTTCTCCC 7 0.17500000000000002 No Hit ATCGAGCGCTACCCCAGAAAAGTGACGCGCCGTATGGGCCAGAAGAAGGT 7 0.17500000000000002 No Hit CTTGGCTATGGACATCAACAGAGAAAACTATGAACTGGGTCTCCCGGTGA 7 0.17500000000000002 No Hit ATGTTGCCGAGGTGACAAAGGCAGATTACGATGCCTGCACCACAACTAGT 6 0.15 No Hit GCTGATAATACAAAAATTTTCTTCGGGGACAAGGTGCCGAACATGGTTCT 6 0.15 No Hit GTCGCCGATGTAATCGAACCCGTGAAGGGATTTGGCTGGGGTAAAAACTA 6 0.15 No Hit GCCTACTCGATTGCCCTCTATCTTCACCGATTCTTGGAGAACTCCCTCGC 6 0.15 No Hit GCAGACAACTTGCGACCCGCATCAATGATTTCTTCCTTTCCTTATTTTCT 6 0.15 No Hit GCAATACCCCCTCATATTCTTATTATTCGATTCATCCATTCGTTCGTCCA 6 0.15 No Hit GCCAATCTCCCGATATATAGAGTATTAAAGTTACTCGACGGTCTTTCCGT 6 0.15 No Hit GAAAAGAAGAAGCGGAACAAACGGGCCTGAAAATGGGCTTTGAAATCGGT 6 0.15 No Hit ATGGGTTTATGACCAAGTTAATGAAGGAAAGGACATAGAAGTACAAGAAG 6 0.15 No Hit CTTTCACATCGTACCAAGCTCACAATGAAGCCTTCTACCCTCCTACCAAT 6 0.15 No Hit GTGAAGTTCTAGCCCACCTCTTCAGCATATGTAACTACGAGAAGGACAAC 6 0.15 No Hit CAGTACTTGGGTCCGGCTATCGAATCATTCCTAGGCGACTTAAAGCCAGT 6 0.15 No Hit GGAGAAGAAATGCTATGGTCTTTTCTTGTTGCTGCTCATTGCCCTGGCTT 6 0.15 No Hit ACCGTGTTGCACGAGCTCACTCACGCCATCTCTGGAACTGAGGATGTCGG 6 0.15 No Hit GTGGGATATGGCGGAGACCGCGAAGGGCGGACCTTTCTCTGAGATTCTAG 6 0.15 No Hit CCATCCCTTCCACCATCCGCATGTACACCTCCCTCCTCACGTCCCGATCC 5 0.125 No Hit GGGAGATGAGATGTTGAAGGACATCTTTCGGGACATCAAGCAGAAATTTG 5 0.125 No Hit GGAAAATTGTGAAGGATGGTCTTGCTTTGCCATTGTTGATAGATATTTGT 5 0.125 No Hit GTGCTAAATCGCCGAGATTAAGAGGAGATAAGAGAGATAGAAAGGAGAAG 5 0.125 No Hit CTCACCCACTCGCATCCGCTGGACGGAAACCAACCATCAAAGAATACTAT 5 0.125 No Hit GCCATCTTCTGCGATAACTATACTTTACTACGTCGAAGCAGGAGAGCGAT 5 0.125 No Hit GGAGCCAGCTCAGGAACCAGAAGTCGTCCACCACCACCTCTGCTTCCTCC 5 0.125 No Hit GGGGAGGCGCTTGTGAGGCAGTTTTTGTATGCGCAGAGGTTCTTTGAGAG 5 0.125 No Hit CTTGTACATCCGCAAAGGCCAGTCAGTATTCCAATTATGCAGCCAAATTA 5 0.125 No Hit CTTCGGAGCGAGGCTCGTGGGGCGTTGGAAGAGCGGTGCACCTCTCGACT 5 0.125 No Hit GCGTGTTCTTGGACATCTTCGCCGCAAACCACAAGAGCGGCGTCACTGCC 5 0.125 No Hit GCAAGACCACCTCTACCGACCACCACTCGGACGGTAAGAAGCGCAAGACT 5 0.125 No Hit AGCCAGACCTCGTCGTGGACCGTCACCCATTCATCCACCTTGACCGCCGG 5 0.125 No Hit GTGAGTTCGAGGCTGGTATCTCCAAGGATGGCCAGACCCGTGAACACGCC 5 0.125 No Hit TTTTTAATCAGCTAAGGACAAAAGAGCAGCTTGGCTATGTTGTTGAATGT 5 0.125 No Hit AGGCTTCAGTGCGTCGGCGGCCTTTGCACTTGGCTCGATTGCGTGCGCGT 5 0.125 No Hit GTCATCTGCCCCGCTCCCACAAGTTCAGGCTCCACCTCCGAAGGAGGAAA 5 0.125 No Hit GCACCGGCCCGTCCGCCATTGCGGTGGTCGAGCCACGGATCCGTAGGAGA 5 0.125 No Hit GCTTATGATCCTTTTGCTGATGCAAATGCCGAGGACTCTGGTGCTGGGAC 5 0.125 No Hit GTTTTCCTGGTAGCTCTCCACCACATCCAATGGAAATTGCCTCCCACAAC 5 0.125 No Hit GTGATTGGCTTGATAAGAAGAATACTATTTCCGGAAAGATTACTGGGGAT 5 0.125 No Hit CTGGTGTCGCCATCGTGAACGCACAACCCATTGGCTTGGAATCCGGCTCG 5 0.125 No Hit GTCCAACTCCCTCGATCTGCTCAAGGCCACTGGCACCGTTGTCGTCTCTG 5 0.125 No Hit AGATAAGCTTGATAGATGGGCAGAATCAACAGTTGAAATCTCCCTCCGAC 5 0.125 No Hit GTATCCTCACCCTCAAGTACCCCATCGAGCACGGCATCGTCACCAACTGG 5 0.125 No Hit ACACCAACAGCATCTGCCTCTGATGATACAGCAGTGGCAGGCGTTGTGGG 5 0.125 No Hit CCCATCGACCCTTTCGTCGAAGACTTCATACCACAACCAACACCCCAACC 5 0.125 No Hit GTGAAAGAGAATACAGACTTTGCAGAATTTGCAGCCAGTATTAATGAAGA 5 0.125 No Hit AAAAGATCATGACAAGCCTGTTCAACAGGTGATTATCGAGATGACTGATG 5 0.125 No Hit AATTGTTCCTATTCGATTGCTCAGTACGATTCTCCATTCCAGTTTATCCA 5 0.125 No Hit GGGGAGGAAGGTCGACTTCACGGTGCACAGGCTCGCTCCGTCCTTCATGG 5 0.125 No Hit CACAAGCAGAAATTGATGCAGTTGCTTGGTCTAAGTAGATCAGCAGCATT 5 0.125 No Hit CAAGAATGAAAATAGCTGCTGGGGCAGCGAAAGGATTGGAGCACTTGCAT 5 0.125 No Hit GGAAACAATCTCGACTGCTACAACCAAAAGTCATTCGCAAATGGACTCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.21250000000000002 0.0 0.0 0.0 0.0 88-89 0.3 0.0 0.0 0.0 0.0 90-91 0.35 0.0 0.0 0.0 0.0 92-93 0.4875 0.0 0.0 0.0 0.0 94-95 0.5375000000000001 0.0 0.0 0.0 0.0 96-97 0.575 0.0 0.0 0.0 0.0 98-99 0.6125 0.0 0.0 0.0 0.0 100-101 0.7250000000000001 0.0 0.0 0.0 0.0 102-103 0.825 0.0 0.0 0.0 0.0 104-105 0.9125000000000001 0.0 0.0 0.0 0.0 106-107 1.0125 0.0 0.0 0.0 0.0 108-109 1.225 0.0 0.0 0.0 0.0 110-111 1.8125 0.0 0.0 0.0 0.0 112-113 2.325 0.0 0.0 0.0 0.0 114-115 2.825 0.0 0.0 0.0 0.0 116-117 3.35 0.0 0.0 0.0 0.0 118-119 3.9375 0.0 0.0 0.0 0.0 120-121 4.3875 0.0 0.0 0.0 0.0 122-123 5.175 0.0 0.0 0.0 0.0 124-125 5.925 0.0 0.0 0.0 0.0 126-127 6.4625 0.0 0.0 0.0 0.0 128-129 7.8 0.0 0.0 0.0 0.0 130-131 8.4875 0.0 0.0 0.0 0.0 132-133 9.75 0.0 0.0 0.0 0.0 134-135 11.0625 0.0 0.0 0.0 0.0 136-137 11.8875 0.0 0.0 0.0 0.0 138-139 12.825 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCAGACA 10 0.006830828 145.0 1 CCCTCCC 10 0.006830828 145.0 7 >>END_MODULE Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699697 spots for SRR26075328.sra Written 2699697 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra Read 2699683 spots for SRR26075328.sra Written 2699683 spots for SRR26075328.sra SRR ids: ['SRR26075328.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_3sp1tlhz SRR26075328.sra spots: 53993674 blocks: [[1, 2699683], [2699684, 5399366], [5399367, 8099049], [8099050, 10798732], [10798733, 13498415], [13498416, 16198098], [16198099, 18897781], [18897782, 21597464], [21597465, 24297147], [24297148, 26996830], [26996831, 29696513], [29696514, 32396196], [32396197, 35095879], [35095880, 37795562], [37795563, 40495245], [40495246, 43194928], [43194929, 45894611], [45894612, 48594294], [48594295, 51293977], [51293978, 53993674]] SRR26075328 file size 19944938 SRR26075328 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075328 SRR26075328_1.fastq SRR26075328_2.fastq Input file: SRR26075328_1.fastq Paired file: SRR26075328_2.fastq trimmed: SRR26075328-trimmed-pair1.fastq, SRR26075328-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 20:12:24 2025 >> started Tue Feb 11 20:13:33 2025 >> done (69.256s) 53993674 read pairs processed; of these: 380 ( 0.00%) short read pairs filtered out after trimming by size control 109354 ( 0.20%) empty read pairs filtered out after trimming by size control 53883940 (99.80%) read pairs available; of these: 9136857 (16.96%) trimmed read pairs available after processing 44747083 (83.04%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 29 0.00% 19 34 0.00% 20 31 0.00% 21 64 0.00% 22 48 0.00% 23 76 0.00% 24 80 0.00% 25 85 0.00% 26 80 0.00% 27 108 0.00% 28 104 0.00% 29 112 0.00% 30 91 0.00% 31 89 0.00% 32 125 0.00% 33 100 0.00% 34 116 0.00% 35 106 0.00% 36 137 0.00% 37 135 0.00% 38 161 0.00% 39 149 0.00% 40 126 0.00% 41 207 0.00% 42 172 0.00% 43 245 0.00% 44 225 0.00% 45 229 0.00% 46 251 0.00% 47 252 0.00% 48 286 0.00% 49 326 0.00% 50 406 0.00% 51 353 0.00% 52 441 0.00% 53 397 0.00% 54 450 0.00% 55 507 0.00% 56 594 0.00% 57 630 0.00% 58 701 0.00% 59 778 0.00% 60 933 0.00% 61 1005 0.00% 62 1085 0.00% 63 1189 0.00% 64 1168 0.00% 65 1357 0.00% 66 1408 0.00% 67 1630 0.00% 68 1746 0.00% 69 1958 0.00% 70 2161 0.00% 71 2632 0.00% 72 3116 0.01% 73 3474 0.01% 74 3683 0.01% 75 3967 0.01% 76 4393 0.01% 77 4844 0.01% 78 5183 0.01% 79 6023 0.01% 80 6830 0.01% 81 7856 0.01% 82 8969 0.02% 83 10216 0.02% 84 11382 0.02% 85 12396 0.02% 86 13235 0.02% 87 14349 0.03% 88 15970 0.03% 89 16983 0.03% 90 19653 0.04% 91 21834 0.04% 92 24704 0.05% 93 27628 0.05% 94 30077 0.06% 95 32880 0.06% 96 35327 0.07% 97 37536 0.07% 98 39009 0.07% 99 43910 0.08% 100 46788 0.09% 101 51496 0.10% 102 57564 0.11% 103 62561 0.12% 104 67380 0.13% 105 72600 0.13% 106 75500 0.14% 107 79642 0.15% 108 83522 0.16% 109 86885 0.16% 110 91085 0.17% 111 99125 0.18% 112 104880 0.19% 113 112593 0.21% 114 119522 0.22% 115 126864 0.24% 116 130642 0.24% 117 135215 0.25% 118 137098 0.25% 119 140703 0.26% 120 145198 0.27% 121 149378 0.28% 122 157807 0.29% 123 166512 0.31% 124 175328 0.33% 125 181603 0.34% 126 186495 0.35% 127 189347 0.35% 128 190307 0.35% 129 190627 0.35% 130 196767 0.37% 131 199438 0.37% 132 206228 0.38% 133 214575 0.40% 134 221587 0.41% 135 227539 0.42% 136 230649 0.43% 137 234581 0.44% 138 236270 0.44% 139 235880 0.44% 140 236215 0.44% 141 239742 0.44% 142 242715 0.45% 143 247727 0.46% 144 258338 0.48% 145 262533 0.49% 146 264657 0.49% 147 269537 0.50% 148 267758 0.50% 149 266509 0.49% 150 270010 0.50% 151 44747083 83.04% 53883940 reads passed initial QC criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=26.30 fanout-score-rank=3 prefix-density=0.30 prefix-fanout=26.3 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCGGTTATCTCGTATGCCGTCTTCTGCTTG criterion=fanout-score sequence-density=0.08 sequence-density-rank=21 fanout-score=48.07 fanout-score-rank=1 prefix-density=0.23 prefix-fanout=17.3 sequence=ATCCTTCTTCTC criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=2.84 fanout-score-rank=27 prefix-density=0.22 prefix-fanout=2.8 sequence=ATGTACCCTGACTTAGGTTTCTCAGA criterion=fanout-score sequence-density=0.03 sequence-density-rank=32 fanout-score=20.79 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=5.7 sequence=CTCTCCTCTTTTCTCAGCCACTTATACACCTCTCAGACCACGCTCCACAACTTTCAGTCATCATGATCCAAACCGGCCACTACCAGATCCATTCCAAGGCGACCAACACCCCAGTGGGGCGCCGCTGGGCTGAAGACAAGTCCTTGAACCCAAAGAGGATTCTCGTCCTCCCTAAAGATAACGCAGACGGCCCCCAGCCCTGGATTATCATCAAGGAGGATGGCGATACCTACACTCTCCGTGCAGGAGGTGCTCCCGTTGTCAACATCGACGGGCAGCTGTTCGCTGAGCTCTTGGACCACGCCATTGGCCAGAGGGCGTGGAAGATTGAGGCTCAGCCCCAGCACGGCAAGAACACCTTCACAATCGTGTCCGCCCATGAACAGAACCTTGGGTGGGTCGTTCCTAACGAGGAGCCGTATACGCAGCTAGATGTGAAGCCCCTGATCTCGACAAAGAGCCTTCCCCCTCAGTTCCCCCCCAACGAGCTCTTCGACATTGTCCGTCTTCT SRR26075328 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 20:15:08 Started mapping on | Feb 11 20:15:09 Finished on | Feb 11 21:08:40 Mapping speed, Million of reads per hour | 60.41 Number of input reads | 53883940 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 26723724 Uniquely mapped reads % | 49.59% Average mapped length | 292.92 Number of splices: Total | 25327652 Number of splices: Annotated (sjdb) | 24651304 Number of splices: GT/AG | 24857627 Number of splices: GC/AG | 353432 Number of splices: AT/AC | 23503 Number of splices: Non-canonical | 93090 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 2.89 Insertion rate per base | 0.02% Insertion average length | 2.53 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 916772 % of reads mapped to multiple loci | 1.70% Number of reads mapped to too many loci | 89864 % of reads mapped to too many loci | 0.17% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 47.83% % of reads unmapped: other | 0.70% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 26243444 26243444 26243444 N_multimapping 916772 916772 916772 N_noFeature 831819 26413681 981774 N_ambiguous 316000 1782 154798 UnstrandedReadsAssigned:25575905 PositiveStrandReadsAssigned:308261 NegativeStrandReadsAssigned:25587152 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075328 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075328-trimmed-pair1.fastq SRR26075328-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 53,883,940 reads, 26,831,447 reads pseudoaligned [quant] estimated average fragment length: 208.78 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,166 rounds 52401 SRR26075328.ke.tsv 34699 SRR26075328.se.tsv 87100 total ==> SRR26075328.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1810.22 3003 53.923 Potri.005G024800.1.v4.1 1035 827.22 2287 89.8659 Potri.004G059700.1.v4.1 961 753.229 3 0.129463 Potri.007G009000.2.v4.1 1416 1208.22 0 0 Potri.003G141000.2.v4.1 2943 2735.22 1195 14.2012 Potri.016G087400.1.v4.1 270 90.6446 2604 933.789 Potri.015G069301.1.v4.1 564 357.732 0 0 Potri.010G195200.1.v4.1 1773 1565.22 669 13.8931 Potri.012G127500.1.v4.1 977 769.229 11685 493.768 ==> SRR26075328.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 165 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 440 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 25 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1331 SRR26075328 completed mapping pipeline successfully