Starting /dee2/code/volunteer_pipeline.sh SRR26075329 current disk space = 3052976627712 free memory = 1578814224 SRR26075329 SRAfilesize 93fb3f58d08833b64f930936586a2a05 SRR26075329.sra SRR26075329.sra file validated SRR26075329 is paired end SRR26075329 is conventional basespace SRR26075329 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075329_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.48375 37.0 37.0 37.0 37.0 37.0 2 36.5185 37.0 37.0 37.0 37.0 37.0 3 36.579 37.0 37.0 37.0 37.0 37.0 4 36.637 37.0 37.0 37.0 37.0 37.0 5 36.6655 37.0 37.0 37.0 37.0 37.0 6 36.7005 37.0 37.0 37.0 37.0 37.0 7 36.5615 37.0 37.0 37.0 37.0 37.0 8 36.662 37.0 37.0 37.0 37.0 37.0 9 36.6765 37.0 37.0 37.0 37.0 37.0 10-14 36.60119999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.6238 37.0 37.0 37.0 37.0 37.0 20-24 36.592499999999994 37.0 37.0 37.0 37.0 37.0 25-29 36.459500000000006 37.0 37.0 37.0 37.0 37.0 30-34 36.367599999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.3408 37.0 37.0 37.0 37.0 37.0 40-44 36.3033 37.0 37.0 37.0 37.0 37.0 45-49 35.946200000000005 37.0 37.0 37.0 37.0 37.0 50-54 36.00789999999999 37.0 37.0 37.0 37.0 37.0 55-59 35.7183 37.0 37.0 37.0 37.0 37.0 60-64 35.6879 37.0 37.0 37.0 37.0 37.0 65-69 35.598 37.0 37.0 37.0 37.0 37.0 70-74 35.7348 37.0 37.0 37.0 37.0 37.0 75-79 35.9405 37.0 37.0 37.0 37.0 37.0 80-84 35.9268 37.0 37.0 37.0 37.0 37.0 85-89 35.8084 37.0 37.0 37.0 37.0 37.0 90-94 35.7604 37.0 37.0 37.0 37.0 37.0 95-99 35.7759 37.0 37.0 37.0 37.0 37.0 100-104 35.803999999999995 37.0 37.0 37.0 37.0 37.0 105-109 35.6243 37.0 37.0 37.0 37.0 37.0 110-114 35.5865 37.0 37.0 37.0 37.0 37.0 115-119 35.4564 37.0 37.0 37.0 37.0 37.0 120-124 35.518499999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.418099999999995 37.0 37.0 37.0 37.0 37.0 130-134 35.35080000000001 37.0 37.0 37.0 37.0 37.0 135-139 35.208600000000004 37.0 37.0 37.0 29.8 37.0 140-144 35.1086 37.0 37.0 37.0 25.0 37.0 145-149 35.0641 37.0 37.0 37.0 27.4 37.0 150-151 35.00125 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 3.0 22 8.0 23 2.0 24 7.0 25 6.0 26 20.0 27 18.0 28 9.0 29 23.0 30 37.0 31 64.0 32 62.0 33 146.0 34 172.0 35 442.0 36 2778.0 37 201.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.62089701829115 13.38010523678276 8.218491606113757 35.78050613881233 2 17.8 15.775 34.9 31.525 3 16.575 18.825 33.6 31.0 4 19.825 26.8 24.975 28.4 5 22.975 31.724999999999998 24.75 20.549999999999997 6 22.225 34.475 24.0 19.3 7 13.5 29.125 40.625 16.75 8 18.35 28.075 31.275 22.3 9 19.2 23.400000000000002 34.475 22.925 10-14 19.45889177835567 30.80616123224645 25.91518303660732 23.819763952790556 15-19 19.765 27.889999999999997 27.805000000000003 24.54 20-24 20.119999999999997 28.335 27.485 24.060000000000002 25-29 19.400000000000002 27.87 27.985 24.745 30-34 19.63 27.625 28.155 24.59 35-39 19.3 28.810000000000002 26.905 24.985 40-44 19.705000000000002 27.860000000000003 27.685 24.75 45-49 19.935 27.46 28.33 24.275 50-54 20.43 27.465 27.825 24.279999999999998 55-59 21.135 27.32 27.245 24.3 60-64 20.73 26.784999999999997 28.43 24.055 65-69 20.34 28.29 26.840000000000003 24.529999999999998 70-74 22.264999999999997 27.150000000000002 27.185 23.400000000000002 75-79 22.06 27.435 26.775 23.73 80-84 21.83 26.945000000000004 27.445000000000004 23.78 85-89 22.645 26.474999999999998 26.96 23.919999999999998 90-94 22.345000000000002 26.31 27.96 23.385 95-99 21.095 26.6 27.939999999999998 24.365000000000002 100-104 22.439999999999998 26.229999999999997 27.91 23.419999999999998 105-109 21.98 26.205000000000002 27.075 24.740000000000002 110-114 23.369999999999997 26.31 27.235 23.085 115-119 21.884999999999998 26.825 26.945000000000004 24.345 120-124 22.56 27.36 26.19 23.89 125-129 22.35 27.445000000000004 26.025 24.18 130-134 22.939999999999998 27.224999999999998 25.929999999999996 23.905 135-139 22.54 27.325 26.284999999999997 23.849999999999998 140-144 22.31 27.375 25.990000000000002 24.325 145-149 22.32 27.465 25.91 24.305 150-151 23.1375 28.0625 25.275 23.525 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.5 9 0.5 10 0.0 11 0.0 12 0.5 13 1.0 14 0.5 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 1.5 22 3.0 23 2.0 24 1.5 25 6.5 26 11.0 27 9.5 28 11.0 29 8.0 30 15.0 31 30.0 32 32.5 33 37.5 34 47.5 35 59.5 36 81.5 37 112.5 38 133.0 39 137.5 40 154.5 41 159.0 42 181.0 43 236.5 44 241.0 45 249.5 46 284.5 47 274.0 48 240.5 49 215.5 50 185.0 51 154.5 52 135.5 53 117.5 54 86.5 55 62.5 56 50.0 57 37.0 58 31.5 59 23.0 60 12.5 61 5.5 62 6.5 63 8.0 64 4.5 65 10.5 66 19.0 67 23.0 68 22.5 69 13.0 70 4.5 71 3.5 72 2.0 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.02 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.35 #Duplication Level Percentage of deduplicated Percentage of total 1 62.763268744734624 37.25 2 21.48272957034541 25.5 3 8.593091828138164 15.299999999999999 4 3.6225779275484413 8.6 5 2.148272957034541 6.375 6 0.6739679865206403 2.4 7 0.33698399326032014 1.4000000000000001 8 0.16849199663016007 0.8 9 0.12636899747262004 0.675 >10 0.04212299915754002 0.27499999999999997 >50 0.04212299915754002 1.425 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAACATCTCGTAT 57 1.425 TruSeq Adapter, Index 5 (97% over 37bp) CGGAAGATCTGCCTCGAATACATGGGCTTCTGGGGTCTCTTTCCAATCCA 11 0.27499999999999997 No Hit ACTGAACTTCATCACTGCAGCCCCTCGCCGGATTCACGTTTTGATGCTTC 9 0.22499999999999998 No Hit GCTTGCTTTCTTGATGATAGTGATGTCCAAACTGATCAGCTGTTGGTGCA 9 0.22499999999999998 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAACATCGCGTAT 9 0.22499999999999998 TruSeq Adapter, Index 5 (97% over 37bp) TGCATTTAAAGCGTGTCCTGCTCATTCAGATTACAAGCTGTTCGTCGTTG 8 0.2 No Hit GTATCAACATTATTAGGCGCCCTATGTCTCCTCATGTGACCTCCAAGTGC 8 0.2 No Hit CCTAGATTTATTTAAAATACCTATTGACAACGTAAAGGAGGATTAAGATT 8 0.2 No Hit GTAGCTTTCCACCACGTCGCAACAGAGGGTTGGGCTGTTGTGGGTCTTCT 8 0.2 No Hit CAATAAGCAATAGATAAAAAACAATACAAGGAAAAGAAGCGAGTGTCTTT 7 0.17500000000000002 No Hit GAATCTTTGAACTCCTGTAGAAGAGCTTCCTCTAACAAAAACTGAGACTC 7 0.17500000000000002 No Hit TTTCAATCCCAAGTACTGTTTAGCACGACACTCAAGGCTATCAAATTCAT 7 0.17500000000000002 No Hit CTTCTTAAGAAAAAAACACAGAAATCAAAAGGGGATCCACACTGAACTAA 7 0.17500000000000002 No Hit CTGCCTATGAGTATGAACATAAGACATGGTAAGGGTAAAAAAAATAGTAC 7 0.17500000000000002 No Hit GTAGAAGGTAAGGTTTTCGCCCCAAGAGCGGCGTTGGTGGAGGGATTCCT 7 0.17500000000000002 No Hit CCTAAAATCCATCACACAATTCAAATGCAATGATTACGGTTTAAGCTAGC 7 0.17500000000000002 No Hit TGTTGATAGACTCATGAGTCTTCTTAGTTTTCTTGGCGGCCACCATGGTT 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGCAACATCTCGTTT 6 0.15 TruSeq Adapter, Index 5 (97% over 37bp) GTGAAATCAAGAATTATGAAGACAAAAGGTCGATGTATTCAATGACAATG 6 0.15 No Hit CAGAGAAGACATCAACTGCTGGAAGGATGCCCGTCAAAGACTTCCCATGG 6 0.15 No Hit AGTTGGTGAGAGTCCCATAGTGGGAAGGCAGCTAATTAACAACGCAAACC 6 0.15 No Hit AAGGAAGAGAAGCAACGCTAAAAAGAACAAAACCACCTCCATGAAAGTAA 6 0.15 No Hit CAGGGTTAAGTGTGCCATTGATCTTCCAGTTCTGTGGAGGTGAGATCTGA 6 0.15 No Hit CCCGTTTATCATTTCTCTCCGTTCTTTTTCAACTACAACATCAACATTAA 6 0.15 No Hit ATCGATGTTTGTTCCTCCAAAACTTGCAGCCTCAAAAACTCCATGGAGTT 6 0.15 No Hit CTCCTCTTTAAAGACCCCGGCTTTCCCTCCGATTGTGTACTGCCAAATCC 6 0.15 No Hit CAGTGTTGTGCCAGAGTCAATTATCGTTCCAGCGGTCCTAAAAACTGATG 6 0.15 No Hit GCTTTGAGCTGCTCCCAACCATTCTGTGGAAATCGGCTGGGAAAATGCAA 6 0.15 No Hit GCTAATTTTTCAATACCAAGTTCTTATTTTGACACACAGTCCATTATTTT 6 0.15 No Hit CCTCACTAGAGATCTGCCCTAGCCTTCCTCTTTCCTGCACCAAGCACTAT 6 0.15 No Hit GTCCATAGATATGAAAAAGAGTTGCTCACCAGGGGCTGTAAATGGACGGG 6 0.15 No Hit CTTGGATCGGAAGCCAGAACCAGAATCCTTGTCAAGAGATAGTGTGAGAA 6 0.15 No Hit CTACATATCCCCTTGTCCCTCTAATTCCGGTGAAGGTTTTAGTTTGGTCT 6 0.15 No Hit GGGAAAATAAGTTGTTCATCGCAGAAGATCCACAAAATCCACCCAAATCA 5 0.125 No Hit CCCAACCGCAGTCATTCTTCTGTCCATATAACACAATATTTGTACCTCAT 5 0.125 No Hit CAAGTTTTCTCATTTGCAATTGCATGGATCGCAGGTGCAGTTTGATCCGC 5 0.125 No Hit GTCACCATTAACGAGGGCTGAGTGGTTGATTGACAGAACAATATTAATCG 5 0.125 No Hit CGAGCTTCTGCCCAAGTGATTCTGCGCACAGGGCATCAAATGAACTGAAC 5 0.125 No Hit GGGGAGAGTGTGCCAGTGGGCCACTATCTGGAGTCTGTGTAGAAGCATGA 5 0.125 No Hit GTTAGCCCAAAATCAACAAAAGAAAGGTAGTTCTCGTCCGAAGATGGTGA 5 0.125 No Hit CCTTTTTGCCCTCTCACAGGCAGTTCTCAACCTCCTAAGAGCTCTGGGAC 5 0.125 No Hit GTGCAGAAAGCTTTCAGCTGTGGTAGGTGGAATATTTCATGCATATGATG 5 0.125 No Hit ACTTCACAGCTTCTCCTGAAGTCATCATCATCCTTCTCCGGACTGTATCT 5 0.125 No Hit GCTGAACAACTGTGGAAAGAGAGAGATGTGAGGGATTAGGCAGCTGGTGT 5 0.125 No Hit GTGCTGTGGCAGAGTGAAGCAGTTCAACCTTGCAGACCAATAATACCGCA 5 0.125 No Hit GCCAAATCCTGATAGAGCCCGCAGTCTCCCAGTCACCTGCATGTATATCA 5 0.125 No Hit GGGGATGGTAACGAGGTTAGATTGTTATGAGAAGCCCTGGATCTTGGGTG 5 0.125 No Hit GCTCATCATCATTGTGTTTGGTGGTGACAACCACCTTATCAGCAACCAAG 5 0.125 No Hit CCCAAATCAAAAATCAACACATTCTTCTCACCAACACTGGTCGCCTTCTT 5 0.125 No Hit TGGCTCACCAACATGTGAAGCTGTCACGTTAGCAGCTGTTGTCGCAGCAG 5 0.125 No Hit GTCCATATAACACAATATTTTCTCATGTTGAGTGGGGAATGTATTGGGGT 5 0.125 No Hit ACAAAAATGAAAACTAGACTGGATTTGAAGGTGTTTCAAATACATGATCT 5 0.125 No Hit CTCAACAGTTCCAACAGCAGATCTCAAAGGCCCAGAGTTGCGTTTCGCAT 5 0.125 No Hit CAATGCGCTATCCGTCCTCATTGCTCGATAGAGAGGTCTGTACCACAAGA 5 0.125 No Hit GCAGTAGCTTAGAAACCATGAATTTTAATGCTTTCCTTCTTCACAATGCC 5 0.125 No Hit CTTTAGTATCTTGTTATAGCTGAATTCTTTTTTCAAACCTTGCACAGTTG 5 0.125 No Hit TTTGGTAAGAAGTAGGTATGGAAGTACGTAGAGTTATTTGTTGGTTACGG 5 0.125 No Hit GCACATTGTCATCCTCCACTTGAACCTTGATGTCCCCTGATTTCAGTCCC 5 0.125 No Hit GTTTCCTTATCCTCCGAGTCACCTACGGAGTCCACCATTTGAGAAAGACA 5 0.125 No Hit ATTCCTTGGACAAAAAGTATATTTCAAGCCCTGTCCATACTTTGTACTGA 5 0.125 No Hit CGAACTGGTTTATTACCTCCATCACCTTGAGCATCAAGATGCTCATTGTA 5 0.125 No Hit CTTCACGAACTCTGCGGGGGATAGAAAGCTGCCATGGCAGACGCACATGA 5 0.125 No Hit CATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGT 5 0.125 No Hit AGTAAATTATTGTCCTTTGTTCTTGCTCTCTCACCCTCAAAAACTTGGAT 5 0.125 No Hit CTCAAATTTGCCAAGCAAGTTGTTGTCCCTCGTTCTTGTCCTCTCACCCT 5 0.125 No Hit GCATCATCAGGACTTCAAGCTCCCCCACGTTGTGCACAACAAACTTTTTA 5 0.125 No Hit GTTGTTTACGCTGTCAGCAAAAGAATTGAATACCAAACTGGGGGCAGACA 5 0.125 No Hit GCAGCATAGTAGAGAACCAAACTGTAGTTTGGTTTTGATGGAATCTGGAG 5 0.125 No Hit CTAAGCAATACTATCTGCATCCATGCTAGCTGCAAGAATTCTCCAAGTTA 5 0.125 No Hit CACGCAGCGCTAACCACAACAAGCACAAGATTTAAGCCTTGCAGCAAACA 5 0.125 No Hit ACCAGCTGCATAATGGAGTGCCGTGTTCTTGTTCTTATCCAGTGCATCAA 5 0.125 No Hit GGTTGCACGACGTGGCTTTATAGCTAGTTTATGCATAATAGTTAAGGCTG 5 0.125 No Hit GCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATG 5 0.125 No Hit GTTCTGTATCTTCTTTATTGTCTCCACCACATCGAACTTCATTCTCCTGA 5 0.125 No Hit TCCTTCCTCGCCAGCATCTGCTTTTCTCAGAAGCTCCTTCAACTCATCCT 5 0.125 No Hit TGCCAATCCAAAAACTTCATCTCGTTTCACATCTTCTGGTTTTATTCCAG 5 0.125 No Hit CTCCATTATTGAAACCAACATAGTTTCTCTTTGTTGCCAAGTATAATACT 5 0.125 No Hit CTCTTGTCAATTTCCTCCTTTCAAATTCAAACTCAAGTTTTGAAATGGGT 5 0.125 No Hit GTATAAACCGAGTGCTTCTCAACCAATGCTGGGAGGCCATCTTTATCAGC 5 0.125 No Hit CACTAGAACAGATTGGTCATCGATACTCCTTTGAACCTTCTTTTTGACAG 5 0.125 No Hit GGGATGGGAGGCATGTAATCAGATTTGGCACCGAGAACACGAGCCTTTGT 5 0.125 No Hit GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA 5 0.125 No Hit CCTGCATGTATATCAACTCCTTGGATATGCTTGGAAGCATGTTTGGGAAC 5 0.125 No Hit CACACACCAGCATATGAGCAGAAAAAGAAAACAAAAGAGAAGGGAAAAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.07500000000000001 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.1375 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.2625 0.0 0.0 0.0 0.0 84-85 0.275 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.30000000000000004 0.0 0.0 0.0 0.0 90-91 0.4 0.0 0.0 0.0 0.0 92-93 0.525 0.0 0.0 0.0 0.0 94-95 0.6375 0.0 0.0 0.0 0.0 96-97 0.7 0.0 0.0 0.0 0.0 98-99 0.9375 0.0 0.0 0.0 0.0 100-101 1.2374999999999998 0.0 0.0 0.0 0.0 102-103 1.5499999999999998 0.0 0.0 0.0 0.0 104-105 1.7875 0.0 0.0 0.0 0.0 106-107 2.05 0.0 0.0 0.0 0.0 108-109 2.3625 0.0 0.0 0.0 0.0 110-111 2.675 0.0 0.0 0.0 0.0 112-113 3.2125000000000004 0.0 0.0 0.0 0.0 114-115 3.7625 0.0 0.0 0.0 0.0 116-117 4.175 0.0 0.0 0.0 0.0 118-119 4.5875 0.0 0.0 0.0 0.0 120-121 5.025 0.0 0.0 0.0 0.0 122-123 5.4625 0.0 0.0 0.0 0.0 124-125 6.2625 0.0 0.0 0.0 0.0 126-127 6.775 0.0 0.0 0.0 0.0 128-129 7.35 0.0 0.0 0.0 0.0 130-131 8.15 0.0 0.0 0.0 0.0 132-133 8.925 0.0 0.0 0.0 0.0 134-135 9.4875 0.0 0.0 0.0 0.0 136-137 10.0875 0.0 0.0 0.0 0.0 138-139 10.65 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCCAT 10 0.006830828 145.0 6 AAAAAAA 35 0.0035366106 20.714287 140-144 >>END_MODULE SRR26075329 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075329_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.10125 37.0 37.0 37.0 37.0 37.0 2 36.017 37.0 37.0 37.0 37.0 37.0 3 36.04 37.0 37.0 37.0 37.0 37.0 4 36.164 37.0 37.0 37.0 37.0 37.0 5 36.039 37.0 37.0 37.0 37.0 37.0 6 35.9435 37.0 37.0 37.0 37.0 37.0 7 35.8925 37.0 37.0 37.0 37.0 37.0 8 35.9505 37.0 37.0 37.0 37.0 37.0 9 35.9725 37.0 37.0 37.0 37.0 37.0 10-14 35.919500000000006 37.0 37.0 37.0 37.0 37.0 15-19 35.7462 37.0 37.0 37.0 37.0 37.0 20-24 35.679 37.0 37.0 37.0 37.0 37.0 25-29 35.4583 37.0 37.0 37.0 37.0 37.0 30-34 35.1912 37.0 37.0 37.0 34.6 37.0 35-39 35.011 37.0 37.0 37.0 29.8 37.0 40-44 34.988499999999995 37.0 37.0 37.0 29.8 37.0 45-49 34.8644 37.0 37.0 37.0 25.0 37.0 50-54 34.7648 37.0 37.0 37.0 25.0 37.0 55-59 34.834500000000006 37.0 37.0 37.0 25.0 37.0 60-64 34.867 37.0 37.0 37.0 25.0 37.0 65-69 34.8231 37.0 37.0 37.0 25.0 37.0 70-74 34.70739999999999 37.0 37.0 37.0 25.0 37.0 75-79 34.6982 37.0 37.0 37.0 25.0 37.0 80-84 34.6879 37.0 37.0 37.0 25.0 37.0 85-89 34.7452 37.0 37.0 37.0 25.0 37.0 90-94 34.7231 37.0 37.0 37.0 25.0 37.0 95-99 34.8226 37.0 37.0 37.0 25.0 37.0 100-104 34.7363 37.0 37.0 37.0 25.0 37.0 105-109 34.8311 37.0 37.0 37.0 25.0 37.0 110-114 34.7489 37.0 37.0 37.0 25.0 37.0 115-119 34.765299999999996 37.0 37.0 37.0 25.0 37.0 120-124 34.6282 37.0 37.0 37.0 25.0 37.0 125-129 34.6048 37.0 37.0 37.0 25.0 37.0 130-134 34.6775 37.0 37.0 37.0 25.0 37.0 135-139 34.43035 37.0 37.0 37.0 25.0 37.0 140-144 34.3707 37.0 37.0 37.0 25.0 37.0 145-149 34.3155 37.0 37.0 37.0 25.0 37.0 150-151 34.00975 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 3.0 13 14.0 14 21.0 15 18.0 16 19.0 17 9.0 18 11.0 19 10.0 20 11.0 21 13.0 22 19.0 23 25.0 24 33.0 25 28.0 26 28.0 27 27.0 28 21.0 29 20.0 30 29.0 31 34.0 32 46.0 33 86.0 34 190.0 35 696.0 36 2399.0 37 190.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.93698424606151 22.80570142535634 9.427356839209802 19.829957489372344 2 32.7 25.874999999999996 24.175 17.25 3 26.525 29.925 26.55 17.0 4 30.349999999999998 32.925 20.974999999999998 15.75 5 29.15 34.25 20.150000000000002 16.45 6 25.85 36.625 21.7 15.825 7 27.150000000000002 22.525000000000002 31.6 18.725 8 26.650000000000002 24.9 24.625 23.825 9 26.200000000000003 24.05 28.525 21.224999999999998 10-14 28.255000000000003 28.23 23.200000000000003 20.315 15-19 28.26 27.845 24.240000000000002 19.655 20-24 28.285 28.88 23.485 19.35 25-29 27.250000000000004 28.610000000000003 24.895 19.245 30-34 26.995 27.735 24.775 20.495 35-39 27.450000000000003 28.08 24.795 19.675 40-44 28.804999999999996 26.735 25.264999999999997 19.195 45-49 27.560000000000002 28.634999999999998 24.404999999999998 19.400000000000002 50-54 24.185000000000002 28.575 27.43 19.81 55-59 26.445 27.589999999999996 26.87 19.095000000000002 60-64 28.225 28.105000000000004 25.35 18.32 65-69 26.765 28.970000000000002 25.085 19.18 70-74 25.71 28.749999999999996 25.865 19.675 75-79 24.865000000000002 29.73 25.82 19.585 80-84 26.715 28.395 25.259999999999998 19.63 85-89 26.565 28.205000000000002 25.405 19.825 90-94 25.979999999999997 27.345000000000002 26.855 19.82 95-99 27.16 28.52 25.259999999999998 19.06 100-104 27.05 27.250000000000004 26.33 19.37 105-109 26.805 28.215 25.295 19.685 110-114 26.674999999999997 27.82 25.405 20.1 115-119 27.505000000000003 28.54 24.8 19.155 120-124 27.744999999999997 28.63 25.369999999999997 18.255 125-129 27.474999999999998 28.585 24.805 19.134999999999998 130-134 28.199999999999996 28.765 24.610000000000003 18.425 135-139 27.44137206860343 28.421421071053555 25.086254312715635 19.050952547627382 140-144 27.960592118423683 27.595519103820763 25.33006601320264 19.11382276455291 145-149 28.551420568227293 27.521008403361346 25.03001200480192 18.897559023609446 150-151 27.94448612153038 28.40710177544386 25.23130782695674 18.41710427606902 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 1.5 7 1.5 8 1.0 9 2.0 10 3.0 11 2.5 12 1.5 13 2.0 14 3.5 15 2.5 16 3.0 17 3.0 18 2.5 19 5.0 20 4.0 21 2.0 22 3.5 23 3.5 24 3.5 25 4.0 26 4.5 27 4.5 28 2.5 29 2.5 30 6.0 31 7.5 32 22.0 33 34.0 34 33.0 35 41.5 36 57.5 37 70.5 38 95.5 39 142.0 40 166.5 41 201.0 42 212.5 43 221.0 44 267.5 45 269.0 46 271.0 47 274.5 48 248.5 49 206.5 50 178.5 51 145.0 52 101.5 53 104.0 54 94.5 55 63.5 56 46.5 57 36.5 58 30.0 59 24.0 60 21.5 61 15.0 62 10.0 63 12.0 64 8.5 65 2.0 66 1.5 67 3.0 68 4.0 69 4.0 70 4.5 71 5.0 72 3.0 73 2.0 74 2.5 75 4.0 76 3.5 77 3.0 78 3.0 79 3.0 80 4.5 81 6.5 82 7.0 83 6.5 84 6.5 85 6.0 86 8.0 87 8.0 88 8.0 89 9.0 90 7.0 91 7.0 92 6.0 93 3.5 94 2.0 95 2.0 96 4.0 97 2.5 98 0.5 99 1.5 100 19.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.005 140-144 0.02 145-149 0.04 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.475 #Duplication Level Percentage of deduplicated Percentage of total 1 66.81577877185848 41.075 2 18.747458316388776 23.05 3 8.011386742578285 14.774999999999999 4 3.538023586823912 8.7 5 1.5860105734038226 4.875 6 0.5693371289141927 2.1 7 0.32533550223668156 1.4000000000000001 8 0.16266775111834078 0.8 9 0.12200081333875558 0.675 >10 0.08133387555917039 1.15 >50 0.040666937779585195 1.4000000000000001 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 56 1.4000000000000001 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 35 0.8750000000000001 No Hit AACATTAGCAGCAAGTAAACGAAGATTAATTCTCTGAAGAAAGAAGTAAA 11 0.27499999999999997 No Hit GCTTGCAATCTTGCCAAGCAGGCTTTTGACGAGGCTATTTCTGAGCTGGA 9 0.22499999999999998 No Hit AGCAAAGCCAAGAATCTTGAAATATTTACCTAATCTTCGTGTTTCCCTCT 9 0.22499999999999998 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 9 0.22499999999999998 No Hit GGTGTTTCTGACTGGGACCCCATTCTCGAGGAGTTGCAAGAGATGGGTTT 8 0.2 No Hit TTCCTGGGGTCGCACTCCAGCAAACAAGCAGTGGAGAGGGGATCATGGTA 8 0.2 No Hit CAATCATGGCCTCCTTTCTTTCTTCACACTCTCTTCTTCAACTTGTTCTG 8 0.2 No Hit AAACCAAAACTCAAGGAATCAACTGGGAACTTGTTGAAGCTGCCTAATTC 8 0.2 No Hit CTGTAATGTCCTTTAAGAATACTAAATTTGATGAAAACGAAGATGATCAC 7 0.17500000000000002 No Hit CGATGGCCTGCCAAGTCTGCTAAGTTCATCTTGGATTTACTCAAGAATGC 7 0.17500000000000002 No Hit ATCACTTTTGATAGTCTTAAAAAGAACTCAGCTTTATTGGGTTTGCAAGA 7 0.17500000000000002 No Hit CTCGGACCCGAATCAACCCAAACCGGGTACCCGTCTCTACAACCCCTATC 7 0.17500000000000002 No Hit ATGAGATCAAGGACGCATCAAAACGTGAATCAGGCGATGGGCCGCAGTGA 7 0.17500000000000002 No Hit GCAAGATCAAGTTCTGATGATGATCATGGTTCCGGGACTTCATGTTCATG 7 0.17500000000000002 No Hit ATCCAACCAGATCCATTCCACAAAAGCCCTAGCCTCCTCTTTATATAAGT 7 0.17500000000000002 No Hit GGAACAACTCTTTGGCTTGGCTGTCAATTTGTTTGCTATATGTTCTTGCA 7 0.17500000000000002 No Hit GCTTTTTGGCTTGCCTCCCCGATACCGCGATTCCGTGAGGCAGATCACTC 6 0.15 No Hit GAACAGTGCCGAGATTATTGCCCGGTTGAAGCGAGATTTATTGCCTACAA 6 0.15 No Hit AATGGTCTTCCTGCAGAAAGTCTAGTCTGATTTCCTGGCACTTATATTAG 6 0.15 No Hit TGCTAAGCTGATCTCTGAGGCAACAACAAAGGCTGGTATGGGGTTGATTG 6 0.15 No Hit AATCTATGCGCTGATGGGATGTCCACTTTCATATGTCTTGTGGTACAGAC 6 0.15 No Hit GTTTATCTACATGAAGAGTGCGAGACTCAGATCATCCACTGTGATATAAA 6 0.15 No Hit GGACAGGAACCTTTTGGCCGAATGGAGTATCTGTTAACATCTAGAAGTGA 6 0.15 No Hit CTCGCTCTTTCCAAAGACATCCCTCTTAACCCTACCAAGAATACCTTTAT 6 0.15 No Hit GGGTACTATTGGAAGCAGCAGTAGTGCTGGCTCCAAAACTGGCTATGACT 6 0.15 No Hit CAAATTGGGTTCAAAGCATTCAGAAGGAAACAAAACTTGGCGAGGCTGTG 6 0.15 No Hit CTAGCTTTCTCCTTCTCCTCAAGATATCTCCATCTCCCTCTTTTAGAAAA 6 0.15 No Hit GAGGGAAGTGCCAAGTAGCCACAATGGCCAAGTTTGCTCTGGCTAACCTC 6 0.15 No Hit GTAGCCTTACCATCACAAACTTCCTCAACATACAAAAACCTCTTCTCTTA 6 0.15 No Hit GTTAGAGGTTACTTCTAGATCTGCAGAGGCTGAACTTGGTGTAGATTTTG 6 0.15 No Hit TGGTACAGTGGTGCAGGACCCTGAATTAGGCCAGGTTATTCAACTTCAAG 5 0.125 No Hit GCTGGAGGTGTCATGACTGTCTTGATCCCAAGGAACACCACCATTCCCAC 5 0.125 No Hit ATAGCAAAAGCACATTGGCTTTAGCAGTTGCCATGAGCAGTTCTGAAAGC 5 0.125 No Hit GGCATTTGATCCTAAAGACCGTCCATCTGCTGAAGAGGCTTTAGCTGATC 5 0.125 No Hit GAATCGTCATGCTGGTGATCTGGGAAATGTCACTGTTGGTGATGATGGCA 5 0.125 No Hit GATTTGCTACTTTTGGATGTCACTCCTCTTTCTCTGGGATTGGAAACAGC 5 0.125 No Hit CTACTTCTTGCTATTTCAAGGGATATCATTCTTGCTTGTCGGTCTAGATT 5 0.125 No Hit TGAGACAACATTAGAACTCAAGTCCTCCGCAGAGAAGTTCTACAAAGTGT 5 0.125 No Hit GTAGTAGCCTAGATGGTAGCATCATTGGTCAGGAAACTTCCTCAAGACGA 5 0.125 No Hit CACAACGTTACAAGGTGGATATCAGGGTTGCACCAGGAACTCATGCAACT 5 0.125 No Hit CTCTCTCTGTGAAACGAAGCAGAAGAAAGAAATAAAAGAGGAGGAAATAA 5 0.125 No Hit GAAGATTGCATTAGGAAGGTGAACCAGATTGCTGACAAATACTGGGACTT 5 0.125 No Hit AGGAAATTGATTTCTTTGATTTGCCCAGTCCAACACCAAAGCAGCACCTC 5 0.125 No Hit AGAAAAGGAAATCATCGTACGACAACAATTCCATAAGAGAATTAGAAGTC 5 0.125 No Hit CAAGATTTCTGTCAAGACAAACTGGAGGAGGCCCAAGGGTATTGATTCTA 5 0.125 No Hit GAAGAAGATTCAGAGGGAAGAACTGCATTGCATTTTTCCTGCGGATATGG 5 0.125 No Hit CACTAGACTTCCACCACCACCGATCTCCTCCACAACTCCTCCTCCACTCC 5 0.125 No Hit GATCAGTTCAGTACTCTGGGGCGTATATTGTACAAGGCACCTTCTGATGG 5 0.125 No Hit AGAGAAGTGGCACTGCAAAGAGAGGTCACGTGGTGGGTTTTCAAGGAGGT 5 0.125 No Hit TTCTTCAATGGTTCTCATTAAGATGCGTGAGATCGCTGAAGCTTACCTTG 5 0.125 No Hit CGATGCCCAACCCGAGCTTTTCATTCACATTATTCCTGACAAGGCCAGCA 5 0.125 No Hit TGAAACTGACCAGTGTAGAAAATAGTCCTGAAGTGAAAGGAACTGAAGCA 5 0.125 No Hit GGGGGAGGCTGCTCCAGTTGCCAAAGAAGCCACTGCAAATGGTTCCTCCA 5 0.125 No Hit GGGTCTTGACGCGCCACTCTTCAACACCCTCCAGCACATGATGGATGCAA 5 0.125 No Hit GGGGGGGTCCAGGCTCCAATGATGCCACCCATAGGCGACAAGCTGCAAAG 5 0.125 No Hit GCTGTGGCTCTGACTGCAAGTGCGGCAGTGACTGCAAATGTGGTATGTAC 5 0.125 No Hit ATTGATCCAAGCCAATTTCATGGTTCTCTCAACAAAGGAAAGGATGAAAA 5 0.125 No Hit GGAAGAAGTCATTAAAGATAAGATTTCGAAGGCTTATGTCAGGACTGAAG 5 0.125 No Hit GAAGGACAAGTGATTGCCTGCCACACCGTTGATGTCTGGAAAGAGCAATT 5 0.125 No Hit CCTAATCACCGGATAAGGTCTGAGCTTTCAGGTTGTTGGACTTCCTTGAA 5 0.125 No Hit GGTTGGTTGATGTTTATAAGAAAACCCTCCAGTCTGATGGCATTGCTGGA 5 0.125 No Hit CAAGAAGATTTAGATTCTTCTGGGACAAAGAAAAATGAAAATGCGTGCAG 5 0.125 No Hit CAGGCTTTACCAGAGTTTCAAACCAAGAGATCAGAGGCAAATAATGGGGT 5 0.125 No Hit AACAACAACAGAGAGCTGTCGGAAGAAAGAAATTAGAAGATGCACAATCC 5 0.125 No Hit TTCAATGGGGCACAGCTAAAGGCAGTTTGTGTTGAAGCTGGGATGCTAGC 5 0.125 No Hit AAATGGTTCATTCTGCAGAAGGCAACCCATTCTGGGAAACAAAAGATGCT 5 0.125 No Hit GCTAACACTATATCCATGATGCCCTCCAACCTTCCTGACTGGAGCTCCCC 5 0.125 No Hit GTTCTGGGAGAAGAATGTGCCCTGGCTATCCCCTTGGGACAAAGATGATT 5 0.125 No Hit GAAAAAGATTGGCAAAACCCTCGAAAAAACCTTTGTTCCTGAAGAGAAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.037500000000000006 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.0625 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.1875 0.0 0.0 0.0 0.0 84-85 0.2 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.225 0.0 0.0 0.0 0.0 90-91 0.325 0.0 0.0 0.0 0.0 92-93 0.44999999999999996 0.0 0.0 0.0 0.0 94-95 0.5625 0.0 0.0 0.0 0.0 96-97 0.625 0.0 0.0 0.0 0.0 98-99 0.8625 0.0 0.0 0.0 0.0 100-101 1.1625 0.0 0.0 0.0 0.0 102-103 1.4375 0.0 0.0 0.0 0.0 104-105 1.6375 0.0 0.0 0.0 0.0 106-107 1.875 0.0 0.0 0.0 0.0 108-109 2.1875 0.0 0.0 0.0 0.0 110-111 2.525 0.0 0.0 0.0 0.0 112-113 3.075 0.0 0.0 0.0 0.0 114-115 3.5999999999999996 0.0 0.0 0.0 0.0 116-117 4.025 0.0 0.0 0.0 0.0 118-119 4.425 0.0 0.0 0.0 0.0 120-121 4.862500000000001 0.0 0.0 0.0 0.0 122-123 5.3125 0.0 0.0 0.0 0.0 124-125 6.1125 0.0 0.0 0.0 0.0 126-127 6.6 0.0 0.0 0.0 0.0 128-129 7.175 0.0 0.0 0.0 0.0 130-131 8.0 0.0 0.0 0.0 0.0 132-133 8.8 0.0 0.0 0.0 0.0 134-135 9.4 0.0 0.0 0.0 0.0 136-137 10.0125 0.0 0.0 0.0 0.0 138-139 10.575 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAACATG 10 0.006830828 145.0 5 CAAGAAA 30 0.0017973486 72.5 8 >>END_MODULE Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828155 spots for SRR26075329.sra Written 1828155 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra Read 1828149 spots for SRR26075329.sra Written 1828149 spots for SRR26075329.sra SRR ids: ['SRR26075329.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd__8fld78o SRR26075329.sra spots: 36562986 blocks: [[1, 1828149], [1828150, 3656298], [3656299, 5484447], [5484448, 7312596], [7312597, 9140745], [9140746, 10968894], [10968895, 12797043], [12797044, 14625192], [14625193, 16453341], [16453342, 18281490], [18281491, 20109639], [20109640, 21937788], [21937789, 23765937], [23765938, 25594086], [25594087, 27422235], [27422236, 29250384], [29250385, 31078533], [31078534, 32906682], [32906683, 34734831], [34734832, 36562986]] SRR26075329 file size 13502646 SRR26075329 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075329 SRR26075329_1.fastq SRR26075329_2.fastq Input file: SRR26075329_1.fastq Paired file: SRR26075329_2.fastq trimmed: SRR26075329-trimmed-pair1.fastq, SRR26075329-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 20:41:48 2025 >> started Tue Feb 11 20:42:48 2025 >> done (60.033s) 36562986 read pairs processed; of these: 274 ( 0.00%) short read pairs filtered out after trimming by size control 697079 ( 1.91%) empty read pairs filtered out after trimming by size control 35865633 (98.09%) read pairs available; of these: 5763484 (16.07%) trimmed read pairs available after processing 30102149 (83.93%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 20 0.00% 19 31 0.00% 20 33 0.00% 21 48 0.00% 22 57 0.00% 23 69 0.00% 24 78 0.00% 25 79 0.00% 26 103 0.00% 27 87 0.00% 28 114 0.00% 29 114 0.00% 30 147 0.00% 31 131 0.00% 32 122 0.00% 33 124 0.00% 34 123 0.00% 35 147 0.00% 36 162 0.00% 37 171 0.00% 38 163 0.00% 39 196 0.00% 40 252 0.00% 41 178 0.00% 42 235 0.00% 43 223 0.00% 44 231 0.00% 45 269 0.00% 46 235 0.00% 47 289 0.00% 48 357 0.00% 49 359 0.00% 50 342 0.00% 51 398 0.00% 52 403 0.00% 53 459 0.00% 54 448 0.00% 55 556 0.00% 56 517 0.00% 57 530 0.00% 58 724 0.00% 59 760 0.00% 60 775 0.00% 61 945 0.00% 62 934 0.00% 63 1077 0.00% 64 1100 0.00% 65 1123 0.00% 66 1417 0.00% 67 1421 0.00% 68 1474 0.00% 69 1712 0.00% 70 1900 0.01% 71 2081 0.01% 72 2399 0.01% 73 2649 0.01% 74 3009 0.01% 75 3317 0.01% 76 3482 0.01% 77 3893 0.01% 78 4268 0.01% 79 4835 0.01% 80 5128 0.01% 81 6011 0.02% 82 6899 0.02% 83 7450 0.02% 84 8710 0.02% 85 9320 0.03% 86 9554 0.03% 87 10698 0.03% 88 11371 0.03% 89 12638 0.04% 90 13949 0.04% 91 15693 0.04% 92 17800 0.05% 93 19344 0.05% 94 21613 0.06% 95 23280 0.06% 96 24924 0.07% 97 27221 0.08% 98 28149 0.08% 99 30885 0.09% 100 32844 0.09% 101 35013 0.10% 102 38793 0.11% 103 42212 0.12% 104 45210 0.13% 105 48517 0.14% 106 51397 0.14% 107 52948 0.15% 108 55868 0.16% 109 58484 0.16% 110 59839 0.17% 111 63913 0.18% 112 68686 0.19% 113 72763 0.20% 114 76984 0.21% 115 80021 0.22% 116 82703 0.23% 117 86709 0.24% 118 89014 0.25% 119 89994 0.25% 120 91248 0.25% 121 94955 0.26% 122 99309 0.28% 123 104059 0.29% 124 107909 0.30% 125 110880 0.31% 126 113128 0.32% 127 117385 0.33% 128 116749 0.33% 129 118591 0.33% 130 122715 0.34% 131 122572 0.34% 132 126632 0.35% 133 132412 0.37% 134 134825 0.38% 135 139082 0.39% 136 140917 0.39% 137 140747 0.39% 138 144191 0.40% 139 146107 0.41% 140 145887 0.41% 141 147112 0.41% 142 150661 0.42% 143 154581 0.43% 144 157782 0.44% 145 159066 0.44% 146 162710 0.45% 147 164633 0.46% 148 166288 0.46% 149 165978 0.46% 150 167929 0.47% 151 30102149 83.93% 35865633 reads passed initial QC criterion=sequence-density sequence-density=0.73 sequence-density-rank=1 fanout-score=3.50 fanout-score-rank=25 prefix-density=0.78 prefix-fanout=3.3 sequence=GCATTCTCAGGCAGCCTAAACCTCCTCAAGAACTTGCCACTGCTACG criterion=fanout-score sequence-density=0.05 sequence-density-rank=34 fanout-score=34.31 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=5.7 sequence=AAACTTCCTCAGACGCTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGTCCTCAGCGTACGGCGGGACCTCTGAGAATTGGGATACAGGACCCAAAAGGCTGAAAGGGGGCAGTGAAGTCGGACGGACTCAACGCCCCTCTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACGAGGCAGTTTCCAGAAACGTGTATCACATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCATTCGGACCTACCGTAAGCCTATATTTCGTTTTTCTGAGACCTATCCGAGTTCAGTGCGACCGTACAGCTCTGGAACCCAAAGGTTCGTTTTTTTCTTGGTACCTATTCCTCCAGGAATTACTGACCATAGTGCTCGTACGCTAGTCTAGCCTAGTAAAACCACGATCAGCCGACGGTCTGGATGCCGAC criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=3.85 fanout-score-rank=28 prefix-density=0.40 prefix-fanout=3.3 sequence=TGATTCCAAGCTTCTTT criterion=fanout-score sequence-density=0.02 sequence-density-rank=37 fanout-score=271.93 fanout-score-rank=1 prefix-density=0.45 prefix-fanout=11.7 sequence=GAGAAGAAACACTAAAAAAATCACACAAGCGAAATCTCCAGCGAGAAGAGAAAGATGGATTTCAGAATCATGGGTCTTGACGCGCCACTCTTCAACACCCTCCAGCACATGATGGATGCAAGTGATCATGAGGCAGACAAGTCCTTCAATGCGCCAACACGCACTTACGTACGTGATGCCAAGGCAATGGCATCAACACCAGCTGATGTGAAAGAGTATCCAA SRR26075329 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 20:43:31 Started mapping on | Feb 11 20:43:31 Finished on | Feb 11 20:50:46 Mapping speed, Million of reads per hour | 296.82 Number of input reads | 35865633 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 30536032 Uniquely mapped reads % | 85.14% Average mapped length | 292.63 Number of splices: Total | 22792796 Number of splices: Annotated (sjdb) | 22117873 Number of splices: GT/AG | 22384393 Number of splices: GC/AG | 289151 Number of splices: AT/AC | 31349 Number of splices: Non-canonical | 87903 Mismatch rate per base, % | 0.43% Deletion rate per base | 0.03% Deletion average length | 2.79 Insertion rate per base | 0.03% Insertion average length | 2.81 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 940061 % of reads mapped to multiple loci | 2.62% Number of reads mapped to too many loci | 156694 % of reads mapped to too many loci | 0.44% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 10.93% % of reads unmapped: other | 0.87% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4389540 4389540 4389540 N_multimapping 940061 940061 940061 N_noFeature 804097 30081088 1108392 N_ambiguous 345601 2235 193586 UnstrandedReadsAssigned:29386334 PositiveStrandReadsAssigned:452709 NegativeStrandReadsAssigned:29234054 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075329 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075329-trimmed-pair1.fastq SRR26075329-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 35,865,633 reads, 30,167,305 reads pseudoaligned [quant] estimated average fragment length: 208.381 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,215 rounds 52401 SRR26075329.ke.tsv 34699 SRR26075329.se.tsv 87100 total ==> SRR26075329.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1810.62 5095 67.329 Potri.005G024800.1.v4.1 1035 827.619 3140 90.7788 Potri.004G059700.1.v4.1 961 753.619 11 0.349242 Potri.007G009000.2.v4.1 1416 1208.62 0 0 Potri.003G141000.2.v4.1 2943 2735.62 955 8.35281 Potri.016G087400.1.v4.1 270 89.8354 3131 833.912 Potri.015G069301.1.v4.1 564 357.711 0 0 Potri.010G195200.1.v4.1 1773 1565.62 55 0.840546 Potri.012G127500.1.v4.1 977 769.619 9507 295.565 ==> SRR26075329.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 156 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 551 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 747 Potri.001G452600.v4.1 1285 SRR26075329 completed mapping pipeline successfully