Starting /dee2/code/volunteer_pipeline.sh SRR26075330 current disk space = 3049024614400 free memory = 1582317960 SRR26075330 SRAfilesize bb42311e2b082c1267faaf350145b502 SRR26075330.sra SRR26075330.sra file validated SRR26075330 is paired end SRR26075330 is conventional basespace SRR26075330 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075330_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.59975 37.0 37.0 37.0 37.0 37.0 2 36.5205 37.0 37.0 37.0 37.0 37.0 3 36.699 37.0 37.0 37.0 37.0 37.0 4 36.686 37.0 37.0 37.0 37.0 37.0 5 36.638 37.0 37.0 37.0 37.0 37.0 6 36.638 37.0 37.0 37.0 37.0 37.0 7 36.6265 37.0 37.0 37.0 37.0 37.0 8 36.6015 37.0 37.0 37.0 37.0 37.0 9 36.66 37.0 37.0 37.0 37.0 37.0 10-14 36.629 37.0 37.0 37.0 37.0 37.0 15-19 36.56570000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.6168 37.0 37.0 37.0 37.0 37.0 25-29 36.4825 37.0 37.0 37.0 37.0 37.0 30-34 36.3968 37.0 37.0 37.0 37.0 37.0 35-39 36.347899999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.3436 37.0 37.0 37.0 37.0 37.0 45-49 36.24829999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.1828 37.0 37.0 37.0 37.0 37.0 55-59 36.1158 37.0 37.0 37.0 37.0 37.0 60-64 36.1005 37.0 37.0 37.0 37.0 37.0 65-69 36.044599999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.998000000000005 37.0 37.0 37.0 37.0 37.0 75-79 36.0606 37.0 37.0 37.0 37.0 37.0 80-84 36.0279 37.0 37.0 37.0 37.0 37.0 85-89 35.896 37.0 37.0 37.0 37.0 37.0 90-94 35.8584 37.0 37.0 37.0 37.0 37.0 95-99 35.849000000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.8197 37.0 37.0 37.0 37.0 37.0 105-109 35.787600000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.675200000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.539100000000005 37.0 37.0 37.0 37.0 37.0 120-124 35.5803 37.0 37.0 37.0 37.0 37.0 125-129 35.44160000000001 37.0 37.0 37.0 37.0 37.0 130-134 35.3365 37.0 37.0 37.0 32.2 37.0 135-139 35.245400000000004 37.0 37.0 37.0 32.2 37.0 140-144 35.1815 37.0 37.0 37.0 29.8 37.0 145-149 35.153600000000004 37.0 37.0 37.0 27.4 37.0 150-151 35.00175 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 2.0 19 2.0 20 2.0 21 1.0 22 2.0 23 2.0 24 4.0 25 9.0 26 7.0 27 15.0 28 17.0 29 28.0 30 27.0 31 42.0 32 67.0 33 104.0 34 152.0 35 419.0 36 2908.0 37 189.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 46.85542470558757 15.985968428965172 6.138812327737409 31.01979453770985 2 19.85 14.549999999999999 33.35 32.25 3 17.474999999999998 17.925 29.225 35.375 4 20.8 24.0 27.075 28.125 5 24.224999999999998 29.099999999999998 24.875 21.8 6 23.225 34.050000000000004 22.7 20.025000000000002 7 15.875 28.275 38.9 16.950000000000003 8 18.55 28.000000000000004 30.025000000000002 23.425 9 19.7 25.6 32.9 21.8 10-14 21.19211921192119 28.97789778977898 26.37763776377638 23.452345234523452 15-19 20.5 27.805000000000003 27.584999999999997 24.11 20-24 20.925 28.305000000000003 26.790000000000003 23.98 25-29 21.125 27.565 27.450000000000003 23.86 30-34 20.064999999999998 28.78 27.205000000000002 23.95 35-39 20.265 27.92 27.544999999999998 24.27 40-44 20.5 28.615000000000002 26.83 24.055 45-49 20.915 27.634999999999998 27.295 24.154999999999998 50-54 21.64 28.79 26.625 22.945 55-59 20.74 28.33 27.700000000000003 23.23 60-64 20.73 27.334999999999997 27.93 24.005000000000003 65-69 20.65 28.015 27.975 23.36 70-74 21.605 26.75 27.029999999999998 24.615000000000002 75-79 21.165 27.35 27.12 24.365000000000002 80-84 21.955 27.224999999999998 27.495000000000005 23.325000000000003 85-89 21.740000000000002 27.694999999999997 26.445 24.12 90-94 21.305 27.334999999999997 27.625 23.735 95-99 21.099999999999998 27.834999999999997 27.49 23.575 100-104 21.29 27.29 27.229999999999997 24.19 105-109 21.605 27.375 27.084999999999997 23.935000000000002 110-114 21.545 27.650000000000002 26.445 24.36 115-119 21.86 27.029999999999998 27.1 24.01 120-124 21.495 27.16 27.505000000000003 23.84 125-129 21.905 27.13 27.169999999999998 23.794999999999998 130-134 21.815 27.025 27.125 24.035 135-139 22.384999999999998 27.02 26.640000000000004 23.955000000000002 140-144 22.33 27.18 25.855 24.635 145-149 22.0 27.37 25.990000000000002 24.64 150-151 22.725 27.400000000000002 25.837500000000002 24.0375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 1.0 21 0.0 22 0.0 23 1.0 24 2.5 25 2.5 26 2.5 27 3.0 28 5.5 29 5.5 30 10.5 31 24.0 32 27.5 33 27.0 34 33.0 35 64.5 36 80.5 37 90.0 38 112.5 39 139.0 40 176.5 41 192.5 42 213.5 43 254.5 44 274.5 45 277.5 46 256.5 47 243.5 48 246.5 49 209.0 50 172.0 51 151.5 52 127.0 53 115.0 54 105.5 55 81.0 56 55.0 57 43.5 58 39.5 59 26.5 60 14.5 61 9.0 62 11.0 63 17.5 64 13.5 65 7.0 66 7.5 67 8.0 68 5.0 69 1.5 70 2.5 71 2.0 72 2.5 73 1.5 74 0.0 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.875 #Duplication Level Percentage of deduplicated Percentage of total 1 64.7713717693837 40.725 2 21.431411530815108 26.950000000000003 3 7.8727634194831015 14.85 4 3.4990059642147116 8.799999999999999 5 1.3916500994035785 4.375 6 0.5566600397614314 2.1 7 0.3976143141153081 1.7500000000000002 8 0.039761431411530816 0.2 9 0.0 0.0 >10 0.039761431411530816 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCCTGTGGGGCGGCCCCACCACCCTCTGCAGCCCGACGAAGGAGTTCTC 10 0.25 No Hit CCATGATCATACGGCTTGCACAGGCAGGCAAAAACTTGGCATGAAATTCA 8 0.2 No Hit GGCTGCAATGGGAAGAATGGACATGACCCTGAGGGATTTTATTTTCTGAA 7 0.17500000000000002 No Hit AAGGAAATGTTGGCACCCACGAGCTTGATGAGCCCGGGCTTCTTCTCCTT 7 0.17500000000000002 No Hit TATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGG 7 0.17500000000000002 No Hit GTCCAGACAGCAATATACGCCAACCAAAAGCTGCACATGTCAAATTTCAT 7 0.17500000000000002 No Hit GCCCATCAAGGTCTTGGCCGTTCATTCCATCAATCGCATCTCTCATTGCC 7 0.17500000000000002 No Hit CTACTGTAAAAATAAGTTCCAGGGTACAGCAAAAACCTTCCCATTATAAG 7 0.17500000000000002 No Hit GTGTAACCTGCACCGGAAAGAACCAGCATGGGAGGTTGGAGAGCATAGGC 7 0.17500000000000002 No Hit GGCAGTTTTTGCATCAAAATTATCGTTAAGGAAGTCTCCGTAAGCTTTAT 7 0.17500000000000002 No Hit GAGGTTTTGGAGAAATGTAGAGCAGTGGTCTTGGAGAGTGGTAAAGGCAT 7 0.17500000000000002 No Hit GCTTATTGGTCGAGATCGATGACCCCTTCTATTACTCCGTGCTAAGGGCT 7 0.17500000000000002 No Hit GTTCATTGTTATTTGTCGTGGAAAAGAAACAGTGCTTCAGGGAAATAACA 6 0.15 No Hit CCGCAAACTGGAGAACAGGACGGGGAAAAAAGCACCATCAAAGATTCAAG 6 0.15 No Hit CTGACGTCAAAACCACTAACAAGCTAAGAGCAAGTGAGAAGAACATGTCA 6 0.15 No Hit ATGGCATGGCAGCATGCCTTCTATACAGCTTTCCATGATGTAAGAGGTGG 6 0.15 No Hit GCTATCATCATTTCTTCGTGCCGTACATGGAGATTGGAGCCTTTTTTCTT 6 0.15 No Hit CCCAGAGCAATTTCCCTAGCCAGAATTGATCCTTAGAGAGCATAACTTTT 6 0.15 No Hit CCCAGGAGATCCCGAGGCACAAACAAGACACAGAGGCGAATGAGCAGGAG 6 0.15 No Hit AGCTAGGTAAAATATAATGATCTCAAAGCTAAACTTTTACTTTGTGCAAG 6 0.15 No Hit CTCGCAAACAGAGATGAAGACCTGGTAGGCAGCTAAGGTGTTCACAATCA 6 0.15 No Hit GGCCATATTCGTTCTCCGTTTTGATGAACCCTGTTCCTGTCGTGTGGTGT 6 0.15 No Hit GTAGACGAACCATTAGTGTACCCCGGTGGTCTTACTATCATGATACTCCG 6 0.15 No Hit GGGGAGTTGACTTCAGATTGGACCGGAGACTTCTCCACTACAACAGCCTC 6 0.15 No Hit GGTCTTTATCTCTAGCCAGTGCCAGGATTCTTCCAGGAGTTCCAACAACA 6 0.15 No Hit GTCGTACACCGATGTGTGAGCACCCCTGAGAGACGGAGTATTGGAAACAG 6 0.15 No Hit GCTTGTGTTCCCCAGCTCCCAAATTGACATTAACAAGCTCAAATTCTACC 5 0.125 No Hit GCCACATAAAAGAGAGTAGACATTGAATTTGTTACATAAAATCACTTCGC 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAACCATCTCGTAT 5 0.125 TruSeq Adapter, Index 11 (97% over 38bp) CCCGAGGTTAAGAAGTCCAGCAACTGTGCTGATGCTGATATCAAAATCCA 5 0.125 No Hit GGAGACTGGCCTTCCCATACTTGACAGGCAGATACCACCTGGAAGGCACA 5 0.125 No Hit GGCCATTCACGGCAGAGACAGTGTTGCCCCTCAGGGATGCCCCTATAAAC 5 0.125 No Hit CCCTGTCAGCTTTAACCTTCATTCCACCAGTAATGCGAACTAGGGTTTCC 5 0.125 No Hit GAGCAAGGCACCACCCAGGTGAATTTGGTGGCAGAGGTCCATAGTCAGGA 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCTAACCATCTCGTTT 5 0.125 TruSeq Adapter, Index 11 (97% over 38bp) GGCACGCCATGCTTCAGCAACAAGCAACATGGTTGATTTCTCAGGCTGAA 5 0.125 No Hit CATAAACTGTAAAGATAAATTGTAAACTGTTCTCAAAACTTGAAGAGAAG 5 0.125 No Hit GTATGGAGCAGCATGTTGTGCGCTGCCGTATGGAGCAGTAGGTTGTGGTG 5 0.125 No Hit GGACTAGATTTGTTGAGTAGTTAAGGGATTGATTGCCTTGTCCAAGTGAC 5 0.125 No Hit AGAGGACGACTTCAAAGTCATGCTTCAATGCCTCCAAGCTTCTATAGTAG 5 0.125 No Hit CGAGTACCACGACCGCGCTTGGTGCAGTAAGCCACACCTCGCTGCTCGCT 5 0.125 No Hit CCTCAAATCCCGCCACGAAACTAAGCGATTGAACTCTTGCCTGGTTACTG 5 0.125 No Hit GTATCAAGCTAGGGTATAAAAGCTTCAACAATTTTTTTACAGGTAACGCC 5 0.125 No Hit GTACTGAACTCGTTGCACGAAAGCGCTTAGATATATATTATACAAGTACT 5 0.125 No Hit AACCTGTGTGATCTTCGACTTCGGAATCTGGACCGCATCTTATCCCACAT 5 0.125 No Hit GCCGATTAAGCCTGTCGCTGCTATGTTTATAGCTAAGCAAATAAACAGCT 5 0.125 No Hit ACCACTTGGAACGGCAGCTCTAGCATAGCTTCCATCAGAGAGCAAAATAT 5 0.125 No Hit GGCTCTCTCGGTCCACTCTCAATGATGAAACATGTGGTTCAACCTTTACC 5 0.125 No Hit GCCTTTCGGGTCTTGGTAAAATTAGTTTTGACAATTTGGAAGCTATAAGA 5 0.125 No Hit GACGTTCATTCCAGCAAGTATGACTGGGTGATTGATCTTGCAGAGTTCAG 5 0.125 No Hit CATTGATCCACTGTGCATTTTCTGATGGCCCCTTAGCAATCTTCTCTTGC 5 0.125 No Hit CGCTCAAGCGCCCCTCACCTCCATGCTTGTTGACAAGATCAACCGCTAAT 5 0.125 No Hit ACACTGAACAACAATTTAATTGAGTGCTTTATTTTTCTTTCAGGTGGAAT 5 0.125 No Hit CAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGC 5 0.125 No Hit ACCACCTCAAGAACTTTCTGTGCAACAGATCCTCCAGCAAGAATAAATGC 5 0.125 No Hit GAAAAGAATATCCAACAAGAACTCGTCGGAGTGTAGCATGGCCCTCTTGA 5 0.125 No Hit CAGCACGTCCCATGGGGGTCCGATAAGCAAGCTCCTTCACAACACTTTCA 5 0.125 No Hit CATCCTTGTACAAACCAGTATGAAAGCACAACGTACTTCACACTTGACAA 5 0.125 No Hit CACGTGTTCCACACCAGGACAGGATGGAGCATGCTCTATAACATGTGGCA 5 0.125 No Hit ATCCTCATGCTCAGCAAACACAAATCCATTCCCTCTACTCCTTGCCCTTC 5 0.125 No Hit GCATAAGTGCCAGTAATGGTGCTGCTTTGGCCAGCTGCTAATAATCCAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0125 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.05 0.0 0.0 0.0 0.0 86-87 0.05 0.0 0.0 0.0 0.0 88-89 0.075 0.0 0.0 0.0 0.0 90-91 0.0875 0.0 0.0 0.0 0.0 92-93 0.1 0.0 0.0 0.0 0.0 94-95 0.1375 0.0 0.0 0.0 0.0 96-97 0.2875 0.0 0.0 0.0 0.0 98-99 0.42500000000000004 0.0 0.0 0.0 0.0 100-101 0.55 0.0 0.0 0.0 0.0 102-103 0.725 0.0 0.0 0.0 0.0 104-105 0.825 0.0 0.0 0.0 0.0 106-107 1.025 0.0 0.0 0.0 0.0 108-109 1.1125 0.0 0.0 0.0 0.0 110-111 1.25 0.0 0.0 0.0 0.0 112-113 1.4125 0.0 0.0 0.0 0.0 114-115 1.575 0.0 0.0 0.0 0.0 116-117 1.8875 0.0 0.0 0.0 0.0 118-119 2.3875 0.0 0.0 0.0 0.0 120-121 3.3499999999999996 0.0 0.0 0.0 0.0 122-123 3.55 0.0 0.0 0.0 0.0 124-125 3.8375 0.0 0.0 0.0 0.0 126-127 4.4125 0.0 0.0 0.0 0.0 128-129 5.1625 0.0 0.0 0.0 0.0 130-131 6.012499999999999 0.0 0.0 0.0 0.0 132-133 6.975 0.0 0.0 0.0 0.0 134-135 7.5375 0.0 0.0 0.0 0.0 136-137 8.125 0.0 0.0 0.0 0.0 138-139 8.725000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTTATT 10 0.006830828 145.0 145 >>END_MODULE SRR26075330 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075330_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2715 37.0 37.0 37.0 37.0 37.0 2 36.3075 37.0 37.0 37.0 37.0 37.0 3 36.2175 37.0 37.0 37.0 37.0 37.0 4 36.3435 37.0 37.0 37.0 37.0 37.0 5 36.3415 37.0 37.0 37.0 37.0 37.0 6 36.178 37.0 37.0 37.0 37.0 37.0 7 36.3365 37.0 37.0 37.0 37.0 37.0 8 36.1875 37.0 37.0 37.0 37.0 37.0 9 36.315 37.0 37.0 37.0 37.0 37.0 10-14 36.20399999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.21229999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.125 37.0 37.0 37.0 37.0 37.0 25-29 35.9809 37.0 37.0 37.0 37.0 37.0 30-34 35.8825 37.0 37.0 37.0 37.0 37.0 35-39 35.7947 37.0 37.0 37.0 37.0 37.0 40-44 35.8069 37.0 37.0 37.0 37.0 37.0 45-49 35.6726 37.0 37.0 37.0 37.0 37.0 50-54 35.5735 37.0 37.0 37.0 37.0 37.0 55-59 35.5911 37.0 37.0 37.0 37.0 37.0 60-64 35.6824 37.0 37.0 37.0 37.0 37.0 65-69 35.5728 37.0 37.0 37.0 37.0 37.0 70-74 35.4486 37.0 37.0 37.0 37.0 37.0 75-79 35.4217 37.0 37.0 37.0 37.0 37.0 80-84 35.4538 37.0 37.0 37.0 37.0 37.0 85-89 35.4341 37.0 37.0 37.0 37.0 37.0 90-94 35.2916 37.0 37.0 37.0 37.0 37.0 95-99 35.312400000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.2449 37.0 37.0 37.0 34.6 37.0 105-109 35.2906 37.0 37.0 37.0 37.0 37.0 110-114 35.1417 37.0 37.0 37.0 32.2 37.0 115-119 35.2097 37.0 37.0 37.0 32.2 37.0 120-124 35.129 37.0 37.0 37.0 32.2 37.0 125-129 35.0565 37.0 37.0 37.0 25.0 37.0 130-134 35.032000000000004 37.0 37.0 37.0 25.0 37.0 135-139 34.8808 37.0 37.0 37.0 27.4 37.0 140-144 34.9196 37.0 37.0 37.0 25.0 37.0 145-149 34.8786 37.0 37.0 37.0 25.0 37.0 150-151 34.63975 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 3.0 13 9.0 14 10.0 15 10.0 16 8.0 17 3.0 18 3.0 19 7.0 20 3.0 21 5.0 22 12.0 23 11.0 24 9.0 25 13.0 26 16.0 27 21.0 28 10.0 29 20.0 30 19.0 31 40.0 32 51.0 33 98.0 34 194.0 35 718.0 36 2544.0 37 163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.975 22.925 8.9 20.200000000000003 2 29.025000000000002 23.925 28.9 18.15 3 23.474999999999998 25.900000000000002 32.0 18.625 4 25.55 33.35 23.025000000000002 18.075 5 27.3 36.4 19.1 17.2 6 24.325 36.4 21.125 18.15 7 24.0 24.2 34.225 17.575 8 23.425 24.85 26.674999999999997 25.05 9 24.85 25.074999999999996 26.174999999999997 23.9 10-14 26.240000000000002 28.615000000000002 24.735 20.41 15-19 25.35 28.34 25.650000000000002 20.66 20-24 24.834999999999997 28.384999999999998 26.05 20.73 25-29 26.08 26.875 26.605 20.44 30-34 25.66 27.66 26.284999999999997 20.395 35-39 25.25 28.38 25.605 20.765 40-44 25.47 27.860000000000003 25.94 20.73 45-49 25.259999999999998 28.645 26.450000000000003 19.645000000000003 50-54 24.46 27.555000000000003 26.645000000000003 21.34 55-59 24.73 27.779999999999998 26.71 20.78 60-64 25.374999999999996 27.38 26.640000000000004 20.605 65-69 25.424999999999997 27.134999999999998 26.56 20.880000000000003 70-74 24.68 29.425 25.545 20.349999999999998 75-79 24.715 27.375 26.68 21.23 80-84 24.625 26.91 27.16 21.305 85-89 25.305 27.465 26.575 20.655 90-94 24.79 28.405 25.89 20.915 95-99 26.150000000000002 27.985 26.025 19.84 100-104 25.035 27.48 26.669999999999998 20.815 105-109 25.230000000000004 27.3 26.790000000000003 20.68 110-114 25.52 27.125 27.02 20.335 115-119 25.305 27.97 26.715 20.01 120-124 25.75 26.97 26.740000000000002 20.54 125-129 25.990000000000002 27.779999999999998 26.119999999999997 20.11 130-134 25.540000000000003 27.365000000000002 26.534999999999997 20.560000000000002 135-139 26.174999999999997 27.195000000000004 26.82 19.81 140-144 26.775 28.18 25.569999999999997 19.475 145-149 26.525 27.05 26.490000000000002 19.935 150-151 27.487499999999997 28.3625 25.937500000000004 18.212500000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.5 9 2.0 10 1.5 11 0.5 12 0.0 13 0.0 14 0.5 15 1.0 16 0.5 17 0.0 18 1.5 19 1.5 20 0.0 21 0.0 22 0.0 23 0.5 24 2.0 25 2.5 26 2.0 27 3.5 28 7.0 29 8.5 30 7.5 31 13.5 32 19.0 33 29.5 34 37.0 35 36.5 36 51.0 37 69.5 38 84.0 39 111.5 40 159.5 41 216.5 42 238.0 43 253.5 44 312.0 45 312.0 46 274.5 47 264.5 48 245.0 49 208.0 50 158.5 51 147.5 52 133.5 53 90.0 54 77.5 55 65.5 56 54.0 57 54.0 58 40.5 59 30.5 60 25.0 61 14.5 62 11.5 63 12.5 64 9.0 65 6.0 66 5.0 67 4.0 68 3.0 69 3.5 70 3.5 71 2.0 72 1.5 73 2.5 74 2.5 75 1.5 76 2.0 77 2.5 78 2.0 79 1.5 80 0.5 81 0.0 82 1.0 83 2.5 84 2.0 85 1.5 86 1.0 87 1.5 88 2.0 89 2.0 90 1.5 91 0.5 92 2.0 93 1.5 94 0.0 95 1.5 96 1.5 97 1.0 98 1.5 99 2.0 100 13.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.6 #Duplication Level Percentage of deduplicated Percentage of total 1 66.23427672955975 42.125 2 20.95125786163522 26.650000000000002 3 7.272012578616352 13.875000000000002 4 3.30188679245283 8.4 5 1.2971698113207548 4.125 6 0.511006289308176 1.95 7 0.2751572327044025 1.225 8 0.039308176100628936 0.2 9 0.0 0.0 >10 0.1179245283018868 1.4500000000000002 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 34 0.8500000000000001 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 14 0.35000000000000003 No Hit CTTACCATCTATTTGAACGACCCACTATGCCACCAAGATTCGGTAGAATT 10 0.25 No Hit ATGCAATCGAACCCGTGAAGGGATTTGGCTGGGGTAAAAACTATCCTGTT 8 0.2 No Hit AGATTCTCCACATTCTGAGGGGATTGGAATGATGCACATGTCGAGGAATT 7 0.17500000000000002 No Hit AGTACAGGTGCTTTGTTGGTGGCCTCGCATGGGCCACTACTGACCAATCC 7 0.17500000000000002 No Hit ATGGACCATGAGGAGGCCAGAAGAAAGAATGTTGGTGGCAAAGTGCTTGG 7 0.17500000000000002 No Hit GTAAATTCCCTCGCCAGATCATTAGCTGCTGAAAACAATATGTTAAAATG 7 0.17500000000000002 No Hit CCTCCCTGTTTTCCCCTCTCCTCTCTCAAATTCTCCAGAGAAAATCATGA 7 0.17500000000000002 No Hit TTCAATCTTTCACTTGGAGGTTGCGGGACCTAAAAGCATGGAGAGAGGGC 7 0.17500000000000002 No Hit TAGAACTCAAGTCCTCCGCAGAGAAGTTCTACAAAGTGTGGAGGAGCCAG 7 0.17500000000000002 No Hit CTTGAAAACTCACCTAATAAACTTTTAAGTAACGAGTTTTTGACAATATA 6 0.15 No Hit TGTATGTCCTATTCCTGGAACCACCAAGATCGAAAACTTCAACCAGAATG 6 0.15 No Hit TGGGAAGCAAGTTTTTCTTGCACGAAAGATAATACAGAGAAATGAAAAAG 6 0.15 No Hit GTACCTCTCTCAATCTCTCTCAGATCTTCTGCTCCTGCTGCAATTTGTAT 6 0.15 No Hit GAGGAAACAAGAGGATATTTCGAGGGAATAGCTCCTAAGCGTCACTCTAA 6 0.15 No Hit AAATACTGTGGTTTTGGGAACTACTTTATGCGCTTTATCCTTTGTCTTCT 6 0.15 No Hit AAGATGCCGAGCTTGGGCCTCCGGCCAAACCCCGGCAAGGAGTCAGGCGG 6 0.15 No Hit CGAGTTGTGGGAACAGGGTCATTTGGTGTAGTCTTCCAGGCAAAATGTTT 6 0.15 No Hit GGAGACATAACGATAGCGACTCTGTCTCAGGGGATCTGCATATGTTTGCA 6 0.15 No Hit GGTTATATACAAAATAGAAAAGGTGGGGCCAGGGGTTAGAGATCAGTACT 6 0.15 No Hit GTTAAGACATCACAGTGATGATTGTGACACTCCTTCTAGGCCTGTTAATG 6 0.15 No Hit GATAAAACCTCTGCTGTTGCCAAGCTTCAGTCTCTTATTTCTCTTACTTC 6 0.15 No Hit GTCACACTAGAGAGTTGGCATACCAGATCTGTCACGAGTTTGAGAGGTTC 6 0.15 No Hit GTCTCAGGAAAAAGAACAGAAAAGAGAAACCTCAGGATCATCGAAGTGTT 5 0.125 No Hit GTAGCGTTGAAGATGGATTTGTAGTCCTCAGTAGGCGATCCACGTTTGTA 5 0.125 No Hit GCAAGCTCACTGACGGAACCAGGCCCCAAATCTGGGACTGCTCGAACAAA 5 0.125 No Hit TGGAGATTTCAATTATCCAGAGAATGGAGAGAACTTGTTGGGGACTGTAC 5 0.125 No Hit GGCTGACTGTGCTGTCCTAATTATTGATTCCACCACTGGTGGTTTTGAAG 5 0.125 No Hit GACAGAGAGCTAGTGTTAGACAGGTTCTTGTTAGACAGAGAGATGGAGAA 5 0.125 No Hit TTCTCCCTCTACGCTTTTGAATACCTGTTATCTTCTTCCATTGAAATCCT 5 0.125 No Hit ACAAGCCCAAGTTTGAGATTGTGAGACCTGAGGAGGCTCCTCGAGTGGTG 5 0.125 No Hit GGGAAGGTCACAGCAGCTACAAAGCAGATTGATCATGACCTGCCACGGAC 5 0.125 No Hit CAGGATCACTATGGGGTTGAAAAGCTAAGGCAAGTTTCACAGAAGTCAAA 5 0.125 No Hit CTAGTGTGCTGCTCTTTGAAGACTCTGCCGGTTTTCACATTTTCAAATCA 5 0.125 No Hit CAAAACTGACCATTGAATACGAAAAACTCCATCCTGAAGTCCCGGTTCCA 5 0.125 No Hit GCGAAGTTTGGAGGAGAGATTTTATGCCTGCCAGATGCATTCCTTTGCTC 5 0.125 No Hit GTTATCAGGGCATGAGTGGGGAGGGGTTTAGGAATGTCGCAGTGAATAAT 5 0.125 No Hit GCTAGATACTAGTAGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGTTC 5 0.125 No Hit GCACAAATCTGTTTGAATATGAATTCGGTACTAAAATTGTGAATTGTGGA 5 0.125 No Hit GTGACGGTAGATTTGCATTGTTGATACGGGAGCCTTTAAGAGAAGGGAAT 5 0.125 No Hit GCTACAGGAAAAGATGAATCTCAGCCAAAAGGAAGTTAAACGATTTAATC 5 0.125 No Hit GGCGAAGAGAGAAGAAAACATAAAAAGAAACAATCAACAGTACTCTCAGA 5 0.125 No Hit AGGGAGATCGACCACAATAAGAAAGACCGGGTTCAAGAAGCTCTCAGATA 5 0.125 No Hit GTTTCAGAGGTCCATGACTATGCCAGGAACTCCAGGGACACCGACGACAC 5 0.125 No Hit GCCTCCGCCCGTCTCTGGGACTATGGACGGGCACGCTCATATCAGGCTAT 5 0.125 No Hit ATTTGGGCGACAACAGTGGGGGGTGCTTAGGACAAAGCTTGGTACATGGA 5 0.125 No Hit AGCTGATAAAGGAGGTTTCCTCCCTGTTTGGTGGGAAACTCAACATTCTT 5 0.125 No Hit GTCAGTGAAGGGGATGCTATAAGTGTATCCTTCACCATGGATCGTTCAAA 5 0.125 No Hit TCACAATAACCCTTTCCATAAAGGAACTGAATACATCTTCTGTTGGGTAT 5 0.125 No Hit TATAAGAAGGAGAACTGTCAATCCTGCCGTGTCGCCTCTCCAAATTGAGA 5 0.125 No Hit AAAGCATTGCTCCTTTCTCTCTCTCCTAACTCCAAAATCGAAATGTCAGG 5 0.125 No Hit CGCTCCGAAGAAGTCGTATGCCTCCGTTGTGAAAGTTCAGAAGGAGTACA 5 0.125 No Hit GCTCGAGAAGGAGCCCAAGTTCTTGAAGAATGGTGATGCTGGGTTTGTGA 5 0.125 No Hit GATGGCCTGGCCAATTAGTCTCAACATCATTATTGGGAATGGTATAGTGC 5 0.125 No Hit GTTCAACCAGGTTTTCAACACAGCAATGTACAACCAAACTACTGTAGTCC 5 0.125 No Hit GTCATGTCAAAGAGAAGGACAAGAGAGCCAAAGGAAGAAAATGTCACTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0125 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.05 0.0 0.0 0.0 0.0 86-87 0.05 0.0 0.0 0.0 0.0 88-89 0.075 0.0 0.0 0.0 0.0 90-91 0.0875 0.0 0.0 0.0 0.0 92-93 0.1 0.0 0.0 0.0 0.0 94-95 0.1375 0.0 0.0 0.0 0.0 96-97 0.2875 0.0 0.0 0.0 0.0 98-99 0.42500000000000004 0.0 0.0 0.0 0.0 100-101 0.55 0.0 0.0 0.0 0.0 102-103 0.7375 0.0 0.0 0.0 0.0 104-105 0.8500000000000001 0.0 0.0 0.0 0.0 106-107 1.0375 0.0 0.0 0.0 0.0 108-109 1.1125 0.0 0.0 0.0 0.0 110-111 1.25 0.0 0.0 0.0 0.0 112-113 1.4375 0.0 0.0 0.0 0.0 114-115 1.6 0.0 0.0 0.0 0.0 116-117 1.9124999999999999 0.0 0.0 0.0 0.0 118-119 2.3875 0.0 0.0 0.0 0.0 120-121 3.2750000000000004 0.0 0.0 0.0 0.0 122-123 3.4749999999999996 0.0 0.0 0.0 0.0 124-125 3.775 0.0 0.0 0.0 0.0 126-127 4.362500000000001 0.0 0.0 0.0 0.0 128-129 5.0625 0.0 0.0 0.0 0.0 130-131 5.887499999999999 0.0 0.0 0.0 0.0 132-133 6.875 0.0 0.0 0.0 0.0 134-135 7.425 0.0 0.0 0.0 0.0 136-137 8.05 0.0 0.0 0.0 0.0 138-139 8.6625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277985 spots for SRR26075330.sra Written 2277985 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra Read 2277973 spots for SRR26075330.sra Written 2277973 spots for SRR26075330.sra SRR ids: ['SRR26075330.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_7ap2ol9a SRR26075330.sra spots: 45559472 blocks: [[1, 2277973], [2277974, 4555946], [4555947, 6833919], [6833920, 9111892], [9111893, 11389865], [11389866, 13667838], [13667839, 15945811], [15945812, 18223784], [18223785, 20501757], [20501758, 22779730], [22779731, 25057703], [25057704, 27335676], [27335677, 29613649], [29613650, 31891622], [31891623, 34169595], [34169596, 36447568], [36447569, 38725541], [38725542, 41003514], [41003515, 43281487], [43281488, 45559472]] SRR26075330 file size 16827711 SRR26075330 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075330 SRR26075330_1.fastq SRR26075330_2.fastq Input file: SRR26075330_1.fastq Paired file: SRR26075330_2.fastq trimmed: SRR26075330-trimmed-pair1.fastq, SRR26075330-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:26:17 2025 >> started Wed Feb 12 02:27:10 2025 >> done (52.187s) 45559472 read pairs processed; of these: 281 ( 0.00%) short read pairs filtered out after trimming by size control 176241 ( 0.39%) empty read pairs filtered out after trimming by size control 45382950 (99.61%) read pairs available; of these: 5899657 (13.00%) trimmed read pairs available after processing 39483293 (87.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 28 0.00% 19 26 0.00% 20 32 0.00% 21 40 0.00% 22 62 0.00% 23 73 0.00% 24 70 0.00% 25 59 0.00% 26 104 0.00% 27 98 0.00% 28 98 0.00% 29 80 0.00% 30 103 0.00% 31 91 0.00% 32 103 0.00% 33 97 0.00% 34 107 0.00% 35 122 0.00% 36 133 0.00% 37 156 0.00% 38 141 0.00% 39 143 0.00% 40 147 0.00% 41 174 0.00% 42 195 0.00% 43 184 0.00% 44 197 0.00% 45 170 0.00% 46 203 0.00% 47 219 0.00% 48 277 0.00% 49 283 0.00% 50 289 0.00% 51 304 0.00% 52 343 0.00% 53 323 0.00% 54 344 0.00% 55 378 0.00% 56 424 0.00% 57 447 0.00% 58 549 0.00% 59 514 0.00% 60 628 0.00% 61 607 0.00% 62 643 0.00% 63 674 0.00% 64 788 0.00% 65 765 0.00% 66 821 0.00% 67 951 0.00% 68 968 0.00% 69 1033 0.00% 70 1060 0.00% 71 1192 0.00% 72 1373 0.00% 73 1554 0.00% 74 1718 0.00% 75 1802 0.00% 76 1993 0.00% 77 2145 0.00% 78 2206 0.00% 79 2477 0.01% 80 2842 0.01% 81 3092 0.01% 82 3531 0.01% 83 3932 0.01% 84 4492 0.01% 85 4787 0.01% 86 5065 0.01% 87 5619 0.01% 88 6169 0.01% 89 6544 0.01% 90 7608 0.02% 91 8289 0.02% 92 9256 0.02% 93 10052 0.02% 94 11449 0.03% 95 12910 0.03% 96 13863 0.03% 97 15203 0.03% 98 16253 0.04% 99 17984 0.04% 100 19206 0.04% 101 21140 0.05% 102 23296 0.05% 103 25870 0.06% 104 28405 0.06% 105 31022 0.07% 106 34061 0.08% 107 36533 0.08% 108 39102 0.09% 109 41771 0.09% 110 43803 0.10% 111 47427 0.10% 112 51277 0.11% 113 54660 0.12% 114 59828 0.13% 115 64848 0.14% 116 67602 0.15% 117 74214 0.16% 118 76894 0.17% 119 78671 0.17% 120 83430 0.18% 121 88154 0.19% 122 93130 0.21% 123 98667 0.22% 124 104377 0.23% 125 109468 0.24% 126 116379 0.26% 127 120299 0.27% 128 124835 0.28% 129 127846 0.28% 130 133570 0.29% 131 136470 0.30% 132 140534 0.31% 133 146072 0.32% 134 152028 0.33% 135 158019 0.35% 136 160941 0.35% 137 166580 0.37% 138 170826 0.38% 139 174332 0.38% 140 177568 0.39% 141 181350 0.40% 142 184898 0.41% 143 188127 0.41% 144 196680 0.43% 145 201690 0.44% 146 204415 0.45% 147 204952 0.45% 148 207762 0.46% 149 209860 0.46% 150 214500 0.47% 151 39483293 87.00% 45382950 reads passed initial QC criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=2.52 fanout-score-rank=32 prefix-density=0.32 prefix-fanout=2.4 sequence=AGGAAACCTCCT criterion=fanout-score sequence-density=0.03 sequence-density-rank=38 fanout-score=38.06 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=9.0 sequence=TCAACAACTTCCCTATCTTTAATCCTCTCACTCCACAAATTCATAAGCTTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAATATGACAAGGAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATGGAGCTATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATTATTTTTAGAAATCCCATACTGCCACCGAATATTCAGTCCTTTAAGAAATCGAACAGCATACCCAACATAGTAAAAACCATCAATAATGCAAATACCGTTACCACAAGTGCAAATACTCCCATTCCTACCT criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=2.33 fanout-score-rank=26 prefix-density=0.37 prefix-fanout=2.3 sequence=TGGTTTTACTAG criterion=fanout-score sequence-density=0.05 sequence-density-rank=28 fanout-score=128.39 fanout-score-rank=1 prefix-density=0.45 prefix-fanout=14.5 sequence=AGAAGAAAAAGGACAAGAAGAAGAATGAAGATGGCCATAGCAGCAGCAGTGACAGCGACTAAAAATCTTGCACTGCTTCCATGCATTAGGTGTGGAGGAGGTCGAGGTCCTGTCTACCAGTGTTGCTGATTATCACTAGAAAAAAGAAAAGAAAA SRR26075330 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:28:00 Started mapping on | Feb 12 02:28:00 Finished on | Feb 12 02:41:52 Mapping speed, Million of reads per hour | 196.37 Number of input reads | 45382950 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 37558671 Uniquely mapped reads % | 82.76% Average mapped length | 295.50 Number of splices: Total | 35783802 Number of splices: Annotated (sjdb) | 34799229 Number of splices: GT/AG | 35093973 Number of splices: GC/AG | 518592 Number of splices: AT/AC | 36640 Number of splices: Non-canonical | 134597 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 3.03 Insertion rate per base | 0.02% Insertion average length | 2.56 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1049124 % of reads mapped to multiple loci | 2.31% Number of reads mapped to too many loci | 117974 % of reads mapped to too many loci | 0.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 14.28% % of reads unmapped: other | 0.39% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6775155 6775155 6775155 N_multimapping 1049124 1049124 1049124 N_noFeature 1118875 37174480 1311779 N_ambiguous 428419 2123 235895 UnstrandedReadsAssigned:36011377 PositiveStrandReadsAssigned:382068 NegativeStrandReadsAssigned:36010997 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075330 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075330-trimmed-pair1.fastq SRR26075330-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 45,382,950 reads, 36,414,570 reads pseudoaligned [quant] estimated average fragment length: 215.149 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,231 rounds 52401 SRR26075330.ke.tsv 34699 SRR26075330.se.tsv 87100 total ==> SRR26075330.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1803.85 3680 45.5585 Potri.005G024800.1.v4.1 1035 820.851 3269 88.935 Potri.004G059700.1.v4.1 961 746.851 0 0 Potri.007G009000.2.v4.1 1416 1201.85 0 0 Potri.003G141000.2.v4.1 2943 2728.85 1616.86 13.2317 Potri.016G087400.1.v4.1 270 84.7296 2660.55 701.226 Potri.015G069301.1.v4.1 564 351.021 0 0 Potri.010G195200.1.v4.1 1773 1558.85 977 13.9963 Potri.012G127500.1.v4.1 977 762.851 50360 1474.24 ==> SRR26075330.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 45 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 432 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 8 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 687 SRR26075330 completed mapping pipeline successfully