Starting /dee2/code/volunteer_pipeline.sh SRR26075331 current disk space = 3053285933056 free memory = 1433112888 SRR26075331 SRAfilesize 0d4a6a954d7a206ecbc431dc4259f4f4 SRR26075331.sra SRR26075331.sra file validated SRR26075331 is paired end SRR26075331 is conventional basespace SRR26075331 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075331_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.52225 37.0 37.0 37.0 37.0 37.0 2 36.501 37.0 37.0 37.0 37.0 37.0 3 36.596 37.0 37.0 37.0 37.0 37.0 4 36.648 37.0 37.0 37.0 37.0 37.0 5 36.7455 37.0 37.0 37.0 37.0 37.0 6 36.614 37.0 37.0 37.0 37.0 37.0 7 36.6465 37.0 37.0 37.0 37.0 37.0 8 36.6215 37.0 37.0 37.0 37.0 37.0 9 36.6175 37.0 37.0 37.0 37.0 37.0 10-14 36.655449999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.606 37.0 37.0 37.0 37.0 37.0 20-24 36.578 37.0 37.0 37.0 37.0 37.0 25-29 36.5148 37.0 37.0 37.0 37.0 37.0 30-34 36.5121 37.0 37.0 37.0 37.0 37.0 35-39 36.4385 37.0 37.0 37.0 37.0 37.0 40-44 36.3963 37.0 37.0 37.0 37.0 37.0 45-49 36.2795 37.0 37.0 37.0 37.0 37.0 50-54 36.2217 37.0 37.0 37.0 37.0 37.0 55-59 36.137699999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.125299999999996 37.0 37.0 37.0 37.0 37.0 65-69 35.993100000000005 37.0 37.0 37.0 37.0 37.0 70-74 36.0687 37.0 37.0 37.0 37.0 37.0 75-79 36.0474 37.0 37.0 37.0 37.0 37.0 80-84 36.1144 37.0 37.0 37.0 37.0 37.0 85-89 35.9473 37.0 37.0 37.0 37.0 37.0 90-94 35.8799 37.0 37.0 37.0 37.0 37.0 95-99 35.91180000000001 37.0 37.0 37.0 37.0 37.0 100-104 35.9011 37.0 37.0 37.0 37.0 37.0 105-109 35.7224 37.0 37.0 37.0 37.0 37.0 110-114 35.597 37.0 37.0 37.0 37.0 37.0 115-119 35.5356 37.0 37.0 37.0 34.6 37.0 120-124 35.651 37.0 37.0 37.0 37.0 37.0 125-129 35.4039 37.0 37.0 37.0 37.0 37.0 130-134 35.3014 37.0 37.0 37.0 32.2 37.0 135-139 35.22430000000001 37.0 37.0 37.0 29.8 37.0 140-144 35.1207 37.0 37.0 37.0 25.0 37.0 145-149 35.1922 37.0 37.0 37.0 27.4 37.0 150-151 35.010000000000005 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 5.0 21 1.0 22 6.0 23 0.0 24 8.0 25 9.0 26 5.0 27 14.0 28 10.0 29 21.0 30 24.0 31 36.0 32 57.0 33 117.0 34 152.0 35 470.0 36 2887.0 37 178.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.846925972396484 15.006273525721456 6.323713927227102 32.82308657465496 2 21.224999999999998 11.774999999999999 33.1 33.900000000000006 3 16.2 17.625 31.175000000000004 35.0 4 20.45 27.05 23.674999999999997 28.825 5 22.875 30.4 25.275 21.45 6 25.0 32.95 22.025 20.025000000000002 7 16.650000000000002 28.7 38.95 15.7 8 18.9 27.3 31.374999999999996 22.425 9 18.325 23.925 32.75 25.0 10-14 19.85099254962748 30.07150357517876 27.27636381819091 22.80114005700285 15-19 20.24 27.125 28.465 24.169999999999998 20-24 19.794999999999998 27.785 28.46 23.96 25-29 19.375 28.205000000000002 28.155 24.265 30-34 20.8 28.03 26.590000000000003 24.58 35-39 20.51 27.689999999999998 27.73 24.07 40-44 18.84 28.075 28.065 25.019999999999996 45-49 19.74 27.839999999999996 27.855 24.565 50-54 19.8 26.75 28.549999999999997 24.9 55-59 19.415 28.16 28.51 23.915 60-64 20.275000000000002 27.060000000000002 27.900000000000002 24.765 65-69 19.755 28.050000000000004 27.185 25.009999999999998 70-74 20.835 28.4 27.205000000000002 23.56 75-79 20.145 27.615000000000002 27.839999999999996 24.4 80-84 20.775 26.935 28.144999999999996 24.145 85-89 20.82 27.775 26.650000000000002 24.755 90-94 20.61 27.295 27.665 24.43 95-99 20.630000000000003 26.875 28.49 24.005000000000003 100-104 20.57 27.189999999999998 27.92 24.32 105-109 21.215 27.485 27.425 23.875 110-114 21.055 27.665 27.089999999999996 24.19 115-119 22.34 26.889999999999997 27.589999999999996 23.18 120-124 21.54 27.22 26.735 24.505 125-129 21.775 27.439999999999998 26.575 24.21 130-134 21.455 27.215 27.58 23.75 135-139 21.615000000000002 27.694999999999997 26.71 23.98 140-144 21.735 27.655 27.169999999999998 23.44 145-149 21.21 27.279999999999998 27.46 24.05 150-151 20.75 27.8125 27.487499999999997 23.95 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 1.0 2 1.0 3 0.0 4 0.5 5 1.0 6 2.0 7 2.0 8 1.0 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 3.0 19 4.0 20 1.5 21 0.5 22 1.0 23 1.5 24 1.5 25 5.5 26 7.0 27 5.5 28 7.5 29 12.0 30 25.5 31 25.5 32 18.5 33 39.0 34 47.5 35 42.0 36 57.5 37 94.0 38 108.0 39 116.0 40 167.5 41 224.0 42 245.0 43 264.0 44 281.5 45 268.0 46 250.5 47 229.5 48 242.0 49 226.5 50 175.5 51 147.0 52 121.5 53 113.5 54 92.0 55 69.5 56 60.5 57 41.0 58 22.5 59 18.0 60 18.0 61 13.5 62 9.0 63 7.0 64 10.5 65 9.5 66 6.5 67 9.0 68 9.0 69 6.0 70 3.5 71 2.5 72 1.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.875 #Duplication Level Percentage of deduplicated Percentage of total 1 63.13152400835072 37.8 2 21.336116910229645 25.55 3 8.01670146137787 14.399999999999999 4 3.8413361169102296 9.2 5 1.9206680584551148 5.75 6 0.8768267223382045 3.15 7 0.5427974947807933 2.275 8 0.12526096033402923 0.6 9 0.08350730688935282 0.44999999999999996 >10 0.12526096033402923 0.8250000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGACAAAGGTAAGCTCGTTCCATCTCTTCCGGGATGCAGTACTATGCCTT 12 0.3 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTGTTATCTCGTAT 11 0.27499999999999997 TruSeq Adapter, Index 4 (97% over 37bp) GACGCGACCAAACCCATAGCCACCACCATCTAGTAACAGAACCATATCCT 10 0.25 No Hit GGCAATTTTTGCATCAAAATTATCGTTAAGGAAGTCTCCGTATGCTTTAT 9 0.22499999999999998 No Hit ATGTGAAAAGAAAATTCAAATAACAAAACTCTAGGCAGCCCCAGCTCCCT 9 0.22499999999999998 No Hit ACTTGAGGTATGTCCTGTCAGCACTTAAAGCTTTATCATATTCATACACT 8 0.2 No Hit CCTGTGATAATCACCCTTCATCTTGAGGTAAAATACCTTGGACTCGGCAG 8 0.2 No Hit GGCTGATTCAAAAGCTGAATATCAACAAAGAGATTGGAGATCACAATTCC 8 0.2 No Hit CTTCAGTCTTGCACACACTAGCACAGTTGTGGCCTCTTACACATGGTCCT 7 0.17500000000000002 No Hit ATAACTATTTGAGCTATTAGACCTAGAAAGAGGCTGGATTGAAATAGGTG 7 0.17500000000000002 No Hit GGCTGTTTAGGTTTAGCAGCTGCTGATTTCTTGGCCTTCTTAGCCCCAGC 7 0.17500000000000002 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.17500000000000002 No Hit GATGAATTTTGCCTGAAAAATCAATATAGCATGAAATATGCGCGACTCTT 7 0.17500000000000002 No Hit ACCTACACCACCTTTGCCTCCAACAAAAACCCACTTGAGAGTGTCTTGGT 7 0.17500000000000002 No Hit GTCCTTGTCCATTAGAGTGCCTGTTCTTTGCTTGTGCAGTACGCCCAACT 7 0.17500000000000002 No Hit GCCTCGAGTTCATCGTCTAAATCGCGTTCGGCGGGTGCTTCAGCAGCGGC 7 0.17500000000000002 No Hit GCTGAGGGCGCTGCCCGTTTAAAACATAGTGGTACTGAAGTCCAAACATA 7 0.17500000000000002 No Hit GCCCAGTCTTTGACCCATCTTTTTTAATCAAAAAGCAGGCTACAAAACCT 7 0.17500000000000002 No Hit CCACGATCTTGATTGAATAGTTGAAACAACAAAGATAACACCTATATATT 7 0.17500000000000002 No Hit AGAATATTTACAGCAGTACGTCCTACAGTTGATCCAAGATAGAACCCATT 7 0.17500000000000002 No Hit ACTGGTTGGAATCTGCCCGCCTTGTATAAATCAACTCCATAATCTTACCC 7 0.17500000000000002 No Hit CTTCATCTGAGATGTCAACCTTTGTCCATTCATACAGCTCCATGTCATAG 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTGTTATCGCGTAT 6 0.15 TruSeq Adapter, Index 4 (97% over 37bp) GGATAGCTAATAAACTCAGAATGTTTCTTAATCAGATCCTTGAGACGGCG 6 0.15 No Hit GCCAGATTCTAACACCAATGCAAACACATCTACAGTTGTCTTGGGAAAAG 6 0.15 No Hit GTCTAATACAAGTTGCCCATCCGGACTTGAAATAATGACCCTTATTGCTG 6 0.15 No Hit GTAGATCTAAATTCCGGGAGTATTTTCCATATTCAATCACCACTGTACAA 6 0.15 No Hit CGGTAATTAATCTTCCTTGTAATACTCCAGGAAGGAGCAGCAAACTCACA 6 0.15 No Hit GTCAGGGATATAAACTGAATACTTCTTGGTTAAGGCACCAACTTGGAATT 6 0.15 No Hit GTCCATGCAGGCACTGTGTTTTCTTTCACAGGATATTTCAGGTCTGTCAT 6 0.15 No Hit GTCTCTTTTAGCTTTTGGTTTTCTCCGATAGTCGAGAGTCCAGGCAGCAG 6 0.15 No Hit GCTAATTATACAAGCAAAATGGAAAACAAACTTCTTCAATTAGGGATGAC 6 0.15 No Hit ATCGGCAACCACATTGTGGAGGAGAAGGAATACACCACACAGAACAAATG 6 0.15 No Hit CAGAGAGGTCTTGACTAACAATGCATCCACGAACAGACTTTCTCCTGCGT 6 0.15 No Hit GGCTTCTTCATAGCTAACCCTCTTAGGTGAATCATCTCCACTACTTGGCT 6 0.15 No Hit CCTCACCGCCCACAAACTCACTACCACCAGCGACAGCATTTCGTGACATA 6 0.15 No Hit CCTAAATTAACTTAGATACATGGTGTTGTTCTAATAATTTTGTTAGTGTG 6 0.15 No Hit ATTGCACTGTTTGCTTAGTTTGCGATGATCATTGACTGGCACCAGCAGAC 6 0.15 No Hit GCAGATCAAGAGGCAAAAACTTCTTGTTCTTGTAAGCCTCCCTCAATACA 6 0.15 No Hit CTAATATCCACGACGGCGATGAAAACAAACACCTAAAATAGCACCACCGC 6 0.15 No Hit CTCTGGGTCTGAAACTCCACTCCTATCGTGGCCTTTGAATGTAAATTGAA 6 0.15 No Hit GCACAATTGAAGGATGTTGCCGGTATTCCCAGCACCTCACCCTCTGCAGA 6 0.15 No Hit CCTAGAATTGTCTTTAAAAACAAAAATTGTTCAAAGTTGAGTACAAAATT 5 0.125 No Hit GCCATCAATCCAAAGCACACAAACAAATCAAGCACGTCAAAGAAAGATAT 5 0.125 No Hit GACCGCATGTGCAGGTAGTGCAAGTGCAGTTAGCGCCGCACTTGCACTTG 5 0.125 No Hit CCTGACCTGAACTGTCCACTCTTGGCACTGGCTTCAACTCATTCATGGGG 5 0.125 No Hit GGTAAAAACAAAACAAGGACATAACTAGCTAACTACTCTACTTGCGACCT 5 0.125 No Hit CTCCCTCAATGTTCTTTCTAAAATGTTCCTTCTGGTCCTCATCAAGTTTC 5 0.125 No Hit CCTTTTAGCCTTGCGCAGTTTCTTGATAAGTGCCATTGGCTTCCTCTTCA 5 0.125 No Hit CTCGCATGCCACCTTGCTGTCAGGGTCTTGCGCCAAGCAAGCATCGAGTA 5 0.125 No Hit GTTAAACCAACTCTTTTCTTGCAAGAGGTGCACCGGCTTGGCCCCTCCTT 5 0.125 No Hit ACCAGATTCTGATAGCTTATAATTTGGAACGAATTCATGCATTGACCTTT 5 0.125 No Hit AATCGGTGTGAGCAGCAAGTGTCCTTCTCATCAAGCACTCTTCCTCACCA 5 0.125 No Hit CCTTCACAAAGACACAGTGAAAAAGTGCTAATTCCACACTGTCTGGAAAT 5 0.125 No Hit CAAGACGTTCAAACTAGCAAAAAACTTGATGGCAACCAAAAATTAAAATC 5 0.125 No Hit CGGCAACCAAACACCAATAGCTTAATTAAAGCATTAAACATCAAGCACCA 5 0.125 No Hit CCATGCTGCCGAAGCATAGATACACTACTGAGGACAGGGGCTGATCATCA 5 0.125 No Hit CTCAGCAACAACATCAGGATGGCTGAAAATTTTTTCTGCATTGTATCTCT 5 0.125 No Hit GCCAAAAACAGCCCAAAGGTCACAGCTGGGTTGATGTGTCCACCTGAGAT 5 0.125 No Hit CTCCGAGATCCCACAACTTTATTGTGACATTGCCTTTTGTAACCTTCCTC 5 0.125 No Hit GTTCTATCAGTGGTTTTCTGTTGGGTGTTTGGTGAGTTGTTGGTAAGAGA 5 0.125 No Hit GTCAGCATTATTTAGGCTTGCTGGCCAAACAGTGAATGGGCAGTTGTTCT 5 0.125 No Hit TCTTCTTTGACCTCCTTATTTTCTCCAATTCCGTTACTCTTCTCTTCAGA 5 0.125 No Hit CCCCCATCATGCTGCAACTTTAGGAAGAAACAGATGCAACGTAGCAAATC 5 0.125 No Hit GGCCTGAAAACAGAAATTTATTTCTCCTAATTCTATCCCAAAAGAACATT 5 0.125 No Hit AAAACCTCAGGATACAGATTACTCCTATGCTCCCCAACTAAGTGATGAGT 5 0.125 No Hit CTTGTTGCAGAAATACCGACTTCATACACGGAGAGTGCCACCAGCTACAG 5 0.125 No Hit CCCAACAACAGAACAATTTAACATGCAGCATCTGGAAGCTTTGAAAAATA 5 0.125 No Hit CCCTCATTAATTGTAGTTGCTACATGTCTTGAAGTCAGCCTGCATCACTC 5 0.125 No Hit CCTATGAGCCCACAAGTAAGGCCATGCCTCACGAAACCACTTGACGAATT 5 0.125 No Hit GTGATGGTGATGGTAGTAGCAGTCCTTTCTGCAATTGGTGGCTCCTAGAA 5 0.125 No Hit GCCTGAGATAGAGGGTATGTGTGATTCACACGAACCCGCAACACGCCAGA 5 0.125 No Hit GCTCCAATAAACAATCACCACAACCACCCAATTCTTGTGGAGGGCAAGAA 5 0.125 No Hit GATGGCTCCAACTTGTCCTTGTACTTTGTGTACAAAAGATCTTCACTCAT 5 0.125 No Hit CCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACT 5 0.125 No Hit ACCATGCTCTTAGGAACTTCAAATGAAGCCAGCACACCTTGGTCACTGGA 5 0.125 No Hit CTCCATATACCTTATGTTCAGGTTGTGAAGGTAGGGGTAATATGGATTCC 5 0.125 No Hit CTCCAGCCAACTGTGCCTGACCAACACCTTCATCTGCTAATTGGTATTCT 5 0.125 No Hit CCATAGAACATGCAAGATGCTCTCTCGTGCAGCATACCCGTGGCTCTCAA 5 0.125 No Hit GCTAAATCTGCCCGGAGAGGCAAGAATTGCACTCTACACAGTCAGGAAAT 5 0.125 No Hit GGTGGTTTCTGTGGAGTTGGCGTCTACGGTTACGAGTTCTGGTTTTATTG 5 0.125 No Hit CTCATCTCCATCCACTCGAGGACTCCCTTTGTGCCTTCGATACCTAGTCT 5 0.125 No Hit GCATCATTCAGTAGTGTCAAACAAACCACTTGATCCACGTCATAATGAAT 5 0.125 No Hit TGTACGTATCAGCTCATGCTCAGTATGAAGTTCGTGTTTCACTTTCTCTG 5 0.125 No Hit TTGGAGGATTTGTTGATTAGCGACTTGTGGATATGAGGGATCACACCACC 5 0.125 No Hit CCTCAAATGGGACACGGACGTACAATTGTCCATGCAAGGTGACCACAGCT 5 0.125 No Hit CATGTGTTTGTGTTTGCAATGTTCTTTCAGATGCCTCTCTAAATGATAAC 5 0.125 No Hit GGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0125 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.037500000000000006 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.075 0.0 0.0 0.0 0.0 100-101 0.1375 0.0 0.0 0.0 0.0 102-103 0.15 0.0 0.0 0.0 0.0 104-105 0.21250000000000002 0.0 0.0 0.0 0.0 106-107 0.32499999999999996 0.0 0.0 0.0 0.0 108-109 0.3875 0.0 0.0 0.0 0.0 110-111 0.625 0.0 0.0 0.0 0.0 112-113 1.025 0.0 0.0 0.0 0.0 114-115 1.15 0.0 0.0 0.0 0.0 116-117 1.4375 0.0 0.0 0.0 0.0 118-119 1.825 0.0 0.0 0.0 0.0 120-121 2.225 0.0 0.0 0.0 0.0 122-123 2.5625 0.0 0.0 0.0 0.0 124-125 2.8875 0.0 0.0 0.0 0.0 126-127 3.2875 0.0 0.0 0.0 0.0 128-129 3.625 0.0 0.0 0.0 0.0 130-131 3.9375 0.0 0.0 0.0 0.0 132-133 4.325 0.0 0.0 0.0 0.0 134-135 4.675 0.0 0.0 0.0 0.0 136-137 5.2 0.0 0.0 0.0 0.0 138-139 5.6875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCACGGT 10 0.006830828 145.0 2 >>END_MODULE SRR26075331 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075331_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.8845 37.0 37.0 37.0 37.0 37.0 2 35.9115 37.0 37.0 37.0 37.0 37.0 3 36.0945 37.0 37.0 37.0 37.0 37.0 4 36.144 37.0 37.0 37.0 37.0 37.0 5 36.0705 37.0 37.0 37.0 37.0 37.0 6 36.074 37.0 37.0 37.0 37.0 37.0 7 36.055 37.0 37.0 37.0 37.0 37.0 8 36.1425 37.0 37.0 37.0 37.0 37.0 9 36.111 37.0 37.0 37.0 37.0 37.0 10-14 35.993399999999994 37.0 37.0 37.0 37.0 37.0 15-19 35.9095 37.0 37.0 37.0 37.0 37.0 20-24 35.799099999999996 37.0 37.0 37.0 37.0 37.0 25-29 35.6465 37.0 37.0 37.0 37.0 37.0 30-34 35.4992 37.0 37.0 37.0 37.0 37.0 35-39 35.5191 37.0 37.0 37.0 37.0 37.0 40-44 35.4568 37.0 37.0 37.0 37.0 37.0 45-49 35.37779999999999 37.0 37.0 37.0 37.0 37.0 50-54 35.174 37.0 37.0 37.0 37.0 37.0 55-59 35.2498 37.0 37.0 37.0 37.0 37.0 60-64 35.3783 37.0 37.0 37.0 37.0 37.0 65-69 35.3374 37.0 37.0 37.0 37.0 37.0 70-74 35.1419 37.0 37.0 37.0 34.6 37.0 75-79 35.0267 37.0 37.0 37.0 27.4 37.0 80-84 35.113600000000005 37.0 37.0 37.0 34.6 37.0 85-89 35.043899999999994 37.0 37.0 37.0 29.8 37.0 90-94 34.9938 37.0 37.0 37.0 25.0 37.0 95-99 35.0427 37.0 37.0 37.0 29.8 37.0 100-104 34.952099999999994 37.0 37.0 37.0 27.4 37.0 105-109 34.942899999999995 37.0 37.0 37.0 27.4 37.0 110-114 34.8351 37.0 37.0 37.0 25.0 37.0 115-119 34.83069999999999 37.0 37.0 37.0 25.0 37.0 120-124 34.7171 37.0 37.0 37.0 25.0 37.0 125-129 34.6682 37.0 37.0 37.0 25.0 37.0 130-134 34.61900000000001 37.0 37.0 37.0 25.0 37.0 135-139 34.5226 37.0 37.0 37.0 25.0 37.0 140-144 34.56535 37.0 37.0 37.0 25.0 37.0 145-149 34.58815 37.0 37.0 37.0 25.0 37.0 150-151 34.214625 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 3.0 13 9.0 14 16.0 15 15.0 16 10.0 17 8.0 18 9.0 19 7.0 20 8.0 21 14.0 22 31.0 23 17.0 24 13.0 25 10.0 26 19.0 27 16.0 28 22.0 29 17.0 30 29.0 31 30.0 32 58.0 33 119.0 34 204.0 35 722.0 36 2425.0 37 169.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.075 21.625 9.15 21.15 2 28.9 25.124999999999996 26.625 19.35 3 24.975 25.85 30.099999999999998 19.075 4 25.8 34.1 21.15 18.95 5 28.15 35.975 18.475 17.4 6 25.25 36.85 21.425 16.475 7 25.025 23.674999999999997 32.574999999999996 18.725 8 24.125 26.924999999999997 25.575 23.375 9 23.75 25.6 27.125 23.525 10-14 26.93 28.76 24.02 20.29 15-19 25.955000000000002 28.244999999999997 25.705 20.095 20-24 26.384999999999998 26.889999999999997 26.46 20.265 25-29 25.380000000000003 28.939999999999998 25.740000000000002 19.939999999999998 30-34 26.045 29.205 24.735 20.015 35-39 25.895000000000003 28.050000000000004 25.25 20.805 40-44 25.814999999999998 28.595 25.345000000000002 20.244999999999997 45-49 25.15 28.645 25.369999999999997 20.835 50-54 25.21 28.884999999999998 26.995 18.91 55-59 25.47 27.165 26.740000000000002 20.625 60-64 25.869999999999997 28.235 25.825 20.07 65-69 25.69 28.205000000000002 26.41 19.695 70-74 25.52 29.21 25.790000000000003 19.48 75-79 24.765 29.459999999999997 26.155 19.62 80-84 26.46 28.71 25.619999999999997 19.21 85-89 25.215 28.03 25.845000000000002 20.91 90-94 25.86 28.335 26.200000000000003 19.605 95-99 24.75 27.655 27.415 20.18 100-104 25.945 28.17 26.355 19.53 105-109 25.119999999999997 27.985 26.61 20.285 110-114 25.540000000000003 28.084999999999997 26.979999999999997 19.395 115-119 25.064999999999998 28.78 26.419999999999998 19.735 120-124 25.345000000000002 28.775000000000002 25.869999999999997 20.01 125-129 25.2 29.065 26.174999999999997 19.56 130-134 26.340000000000003 27.58 26.735 19.345000000000002 135-139 25.955000000000002 28.634999999999998 26.275 19.134999999999998 140-144 26.276313815690784 29.10145507275364 25.871293564678233 18.750937546877346 145-149 26.77901685252788 28.7993198979847 25.10876631494724 19.31289693454018 150-151 26.22827853481685 27.815976997124643 27.15339417427178 18.802350293786724 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 1.0 10 0.5 11 1.5 12 2.0 13 2.0 14 1.5 15 1.5 16 2.0 17 3.0 18 3.0 19 2.0 20 2.5 21 2.0 22 2.5 23 3.0 24 2.0 25 2.5 26 4.5 27 6.0 28 5.5 29 5.0 30 7.5 31 11.0 32 13.0 33 25.5 34 39.0 35 45.5 36 58.0 37 84.5 38 112.0 39 135.5 40 155.5 41 189.0 42 224.0 43 269.5 44 284.5 45 275.5 46 289.0 47 276.0 48 250.0 49 203.5 50 176.5 51 156.5 52 114.5 53 100.5 54 89.5 55 63.0 56 41.5 57 39.5 58 31.5 59 14.5 60 9.0 61 6.5 62 7.0 63 9.0 64 9.0 65 6.0 66 5.5 67 2.5 68 2.5 69 4.5 70 3.0 71 2.5 72 2.5 73 4.0 74 3.0 75 1.0 76 2.5 77 3.0 78 4.0 79 3.5 80 3.0 81 3.5 82 3.0 83 2.5 84 2.5 85 2.0 86 1.5 87 2.0 88 2.0 89 2.0 90 1.5 91 2.0 92 4.0 93 3.0 94 1.0 95 1.5 96 2.0 97 2.0 98 2.5 99 4.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.015 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.8 #Duplication Level Percentage of deduplicated Percentage of total 1 66.46440129449837 41.075 2 19.57928802588997 24.2 3 7.079288025889968 13.125 4 3.762135922330097 9.3 5 1.820388349514563 5.625 6 0.6067961165048543 2.25 7 0.3236245954692557 1.4000000000000001 8 0.16181229773462785 0.8 9 0.12135922330097086 0.675 >10 0.08090614886731393 1.55 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 49 1.225 No Hit GCTGATTTTGGAACTGATTTGGCTATTAGTACTAGAATGGAGCTTGATCC 13 0.325 No Hit CTTAGCTTGGGCTGAAAACACACAGTCATACCCCTCCACCACTCTTTCCC 9 0.22499999999999998 No Hit GTAGAGTATGAGAAAATTAACATGTTCTCTCCAAGTGAAGCACCCAAGGA 9 0.22499999999999998 No Hit GTCCTGGTCAGTGTGGGCGAGCTGATGGATACATCCTGGAGGGCAAAGAA 9 0.22499999999999998 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 8 0.2 No Hit GTTCTTCTGTTTGTGCTGAATGAAAAAAAAAATACAGCCTTCTGGATGTC 8 0.2 No Hit CAAGGACAGGAGTAGACATGGAAACGCCTGTGGTTCCTTGCTTTTATATG 8 0.2 No Hit GAGGGCTTCATGGAGGATCATCTCTTCCATTGAGCAGAAGGAAGAGAGTA 8 0.2 No Hit AAAAGTATTTCACTGGAAGCTTACAATTGGATCCTCCAAAGTGAACCGCA 7 0.17500000000000002 No Hit CCTGTTTATGGAGAAATTTCAGGAACTGCCGTACCCAGCATTTGCCAAAC 7 0.17500000000000002 No Hit CAAGATCAGGGCATCTTACAAGCTTCCAGAGGCGAAAAAGACTAAAGAGG 7 0.17500000000000002 No Hit GAAGCAGGAGTTAAGAGTTGCAGCCTAGGCGACTTGGCTTTGCTTGGAAA 7 0.17500000000000002 No Hit GTTTAGAGAAAAGAAAAGAAAACCCAAAGCTGAAACAAGAACAAAACAAA 7 0.17500000000000002 No Hit CAATCCGCTGTGTCCAGCACGGCGCGACAAGGAGAGGTAGACCCTCACAC 7 0.17500000000000002 No Hit GGAGAGTGGAAAGGAATATATATTGTGTGAGTACAACAGAGACGCTGACT 7 0.17500000000000002 No Hit CCAGCTTCGACAAGGCAGAAGGTGCAGCCACCATGGTGAAGTACTCTAGA 7 0.17500000000000002 No Hit GTTTTGGATACCATAAGATTGATCCTGATTATCCTTGCTGCTGTAATGCT 6 0.15 No Hit CAAGCAGACTGCCGACAAACCTGAAGGTGAAAAACAGGCTAATAAACAAC 6 0.15 No Hit CTTATGTGCCAATATTACTAATGGCAAGCAATGCTGAGCTGAGGCATGGA 6 0.15 No Hit GAGACCGTCAAGAAACCTCAAAAGGGTGAAAAGAAGAACGACACCCCAAC 6 0.15 No Hit GAGACAGAAGCGAAGCAATGGCAAGAATCAAGGTTCATGAGTTGAGACAG 6 0.15 No Hit TCTAAAGCCCGGCAAACTATCTCAAATCTGCAGTGAAGCCTCCTCTAAGC 6 0.15 No Hit GTGCTTACTCTATGGTTCTATGTTGGATGATATTGGCATTGATTTGCCAA 6 0.15 No Hit GCTGAAGAGAAGAGGTTGCTTCAACAGATCGACTTTGCAAGTGAAGAAGT 6 0.15 No Hit GGGAAAAACCCTTTGAACTAAACAAGCTTAGGGATCCACTTATTTTCAAG 6 0.15 No Hit CAATGATGATGAGCAGTATGTGTGGGAATCTCAGGCTGGTGGTTCATTTA 6 0.15 No Hit GCCGGAGGTGGTGACATGGGTGGTGCAATGGATGATGATACTCCCCCAGC 6 0.15 No Hit ACCTCCAGTTCCAGGCTGACAGCTTCCAGAGGCAAGGAAGGCAATTGCGA 6 0.15 No Hit GGCAGGAAGCAGAGTTACGAGGAAGAGGTTGAAGGGTATGGAGGAAGGAG 6 0.15 No Hit GCGCATCAATGGCGTGGAGTAATATGTTCTCATATTTTTCTTTCCCGAAT 6 0.15 No Hit ATGTCGACGAGCTTGAAGAACGCGATTTCGAGGGTGACGAACTCGAGACC 6 0.15 No Hit ATGGGATTATGGGAAGCTTTTCTCAACTGGATACGAAGTCTCTTTTTCAA 5 0.125 No Hit TCAGCTGAAGGAGGTGATGAGGATGAGGGTGTTGATGACCAAGCTGCCAA 5 0.125 No Hit TGAGGTGGTGCAGGTATCATCACCAGAATTGTCAGCACCACCACCAACAA 5 0.125 No Hit CTTCAGCCTGAGTTTCCAGCAATGGCCAATCAAATTCCCTTGGCACTTAC 5 0.125 No Hit TGCATGCAGAGATATCATCCAGGTTCCTCAAAATTTTTGCCGATATCCGC 5 0.125 No Hit TTGCAAAGCATTGGAAAACGAACGAGGTAGAAGCTGTTGAGACAGCTAAG 5 0.125 No Hit CTGAAGAGAGTTCCTTTAAGAGGTTTCCAACATGAGTAAGCTTTCAGGGG 5 0.125 No Hit CAGTGGTGAACCTGAGCATGAGGATAATCCTTGGGGTGTCGCACCATTCA 5 0.125 No Hit ATCAACATCTTATATCCATTCCCGCATAATAATTTTCTTTTAAACACAGG 5 0.125 No Hit CATTTGTAACCCGTGTGAAGACTACAGCTCAGAAAACTGAAGATGATGAA 5 0.125 No Hit ACTCTGCATGGGCTCCTGATCCAGTTACTGGGTATTACAGGCCTGCTAAT 5 0.125 No Hit CACGTTTGGAAACTTCCAGTACTCTGATCTTGGAACTCTTGCCAAAATCA 5 0.125 No Hit GCAACTCTACTCTTTCTCCCTAATCTCTCAAGCTTGAAAGAAATGGCAGA 5 0.125 No Hit GTTAGAGAATACTATTTTTTTGTCTTAAACATTGTTAGTGCAAGAGGAGT 5 0.125 No Hit CTTCTCGCCATCTTATGTGATTCCTCACGGAAAAAAGCAATCTCTTATCT 5 0.125 No Hit TTTATATGACAGCACTTCAAAAGCCACATTTGTCGCTGTTGATGCTGATG 5 0.125 No Hit TGTTAGCCTTTGAGGAGTCACCATACTGATCTTGGGCCATCCACAAACCC 5 0.125 No Hit GTTGAGACCGATGTCGCTGCAGCAGGACAGCCAAAGAAGAGAACGTTCAA 5 0.125 No Hit CATTTATGGAAAGTCCTGGCCGAGCAGTTCGGGATAGAAAAGTACGGGTT 5 0.125 No Hit GTTTGAGAAGGGCCAAAGTAAGCGTATATGGGGTGAACTATACAAAGTTA 5 0.125 No Hit GGGAGACAGAGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAG 5 0.125 No Hit GTTTTGTTTTTACTAGTATTTTTTTTTTTTGAGGAGTTTTATGAAAGGTA 5 0.125 No Hit CTGGACTATCCCTGACAAGTTGACACATGTCCTCAACTACATAAAGTTGC 5 0.125 No Hit AAAACCAACACTTACAATTGTTATCTACGAAGCTTCTCCATGGCTTCGAT 5 0.125 No Hit GGGTGTTACTAAGCCTGGTACCAGCAAATGTTCCACTGTTGGTATTCAAG 5 0.125 No Hit TGCAAGATCCTTCTCTCTCTCTCCCTCTCTCCCTCTCTCCGATTCAATTG 5 0.125 No Hit GGGAGTTCTGACTCCTGGTCGTGTTCGTTTGTTGCTTCACAGAGGCACCC 5 0.125 No Hit CCTCACACCAAGGGGTCGGTACAGTGTTGAACTTCATTTGTCTTTCCTGC 5 0.125 No Hit GCTGGAAGATTTGCTCCTCATCTGGAGAAGTGCATGGGAAAGGGTAGGAA 5 0.125 No Hit AGCACCTGCGACAACTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAA 5 0.125 No Hit GTGGATGATCCAAGAGCCTTGAACAAAATCCTCTACATTAGACCCCCAGC 5 0.125 No Hit CATTGGCATGCTTAAAGACTCGTGGCTACATGGTTTGTTTTGGACAGTCA 5 0.125 No Hit GTTACTGGGTATTACAGGCCTGCTAATTATGTAGCAGAGATCGATCCTGC 5 0.125 No Hit GACAGAACTCTTTGGGAGGCCACGGGTACCTATGTTGAAATGAGTCCTTT 5 0.125 No Hit GGGGAGTGTGGAGGAGAAGAGAGAGACATTCTGGCTATGGAAAAGAAATT 5 0.125 No Hit GTATGCTCGCCCTCATCCTTCCATTAACTACATGCAGCCTCCGATACCAC 5 0.125 No Hit AGAGCCTCCGTCTTCCTCCGACGCAAAATGTCGCAACCGCAACCCTGGGC 5 0.125 No Hit GGGAAATGTATGATCCTGAGGGTTACTCACTGTGGTTCTGCAACTATAAG 5 0.125 No Hit ATAATAAACACATTTGAAGAGCTGGAATCTCATGCGATCAACTCCTTTTC 5 0.125 No Hit GTTTTGTGAGATTGCTAGGTTTGAAAGGCTTGCTTATGCTGACCTATCTG 5 0.125 No Hit TATCAAGGAAGAGTCTGAGGGAAAGATGAAGGGAATCTTAGGCTACACCG 5 0.125 No Hit TTGAGACAATCCATGTCCAAGATGCCACTGGCCATGAGTTTGCCACCCGC 5 0.125 No Hit AATCTGTGTTTTTCTCCCATAAACCCCATACGAAACAAATCAATGGCAGA 5 0.125 No Hit GGCAACGGTTCAGCAACAGTTGAACCTCCTGGACCTCATGATCCATTCTT 5 0.125 No Hit AGATACTAGTAGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGTTCTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0125 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.025 0.0 0.0 0.0 0.0 90-91 0.025 0.0 0.0 0.0 0.0 92-93 0.025 0.0 0.0 0.0 0.0 94-95 0.037500000000000006 0.0 0.0 0.0 0.0 96-97 0.05 0.0 0.0 0.0 0.0 98-99 0.075 0.0 0.0 0.0 0.0 100-101 0.1375 0.0 0.0 0.0 0.0 102-103 0.15 0.0 0.0 0.0 0.0 104-105 0.21250000000000002 0.0 0.0 0.0 0.0 106-107 0.32499999999999996 0.0 0.0 0.0 0.0 108-109 0.3875 0.0 0.0 0.0 0.0 110-111 0.625 0.0 0.0 0.0 0.0 112-113 1.025 0.0 0.0 0.0 0.0 114-115 1.15 0.0 0.0 0.0 0.0 116-117 1.4625 0.0 0.0 0.0 0.0 118-119 1.85 0.0 0.0 0.0 0.0 120-121 2.25 0.0 0.0 0.0 0.0 122-123 2.6375 0.0 0.0 0.0 0.0 124-125 2.9625 0.0 0.0 0.0 0.0 126-127 3.3875 0.0 0.0 0.0 0.0 128-129 3.7249999999999996 0.0 0.0 0.0 0.0 130-131 4.05 0.0 0.0 0.0 0.0 132-133 4.4625 0.0 0.0 0.0 0.0 134-135 4.825 0.0 0.0 0.0 0.0 136-137 5.35 0.0 0.0 0.0 0.0 138-139 5.8375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTAGTG 10 0.006830828 145.0 9 GAGCTAG 10 0.006830828 145.0 7 AGAAAAG 10 0.006830828 145.0 7 >>END_MODULE Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375825 spots for SRR26075331.sra Written 3375825 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra Read 3375820 spots for SRR26075331.sra Written 3375820 spots for SRR26075331.sra SRR ids: ['SRR26075331.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_lqykq51k SRR26075331.sra spots: 67516405 blocks: [[1, 3375820], [3375821, 6751640], [6751641, 10127460], [10127461, 13503280], [13503281, 16879100], [16879101, 20254920], [20254921, 23630740], [23630741, 27006560], [27006561, 30382380], [30382381, 33758200], [33758201, 37134020], [37134021, 40509840], [40509841, 43885660], [43885661, 47261480], [47261481, 50637300], [50637301, 54013120], [54013121, 57388940], [57388941, 60764760], [60764761, 64140580], [64140581, 67516405]] SRR26075331 file size 24942896 SRR26075331 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075331 SRR26075331_1.fastq SRR26075331_2.fastq Input file: SRR26075331_1.fastq Paired file: SRR26075331_2.fastq trimmed: SRR26075331-trimmed-pair1.fastq, SRR26075331-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 19:51:06 2025 >> started Tue Feb 11 19:52:25 2025 >> done (78.550s) 67516405 read pairs processed; of these: 357 ( 0.00%) short read pairs filtered out after trimming by size control 533576 ( 0.79%) empty read pairs filtered out after trimming by size control 66982472 (99.21%) read pairs available; of these: 6390580 ( 9.54%) trimmed read pairs available after processing 60591892 (90.46%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 17 0.00% 19 41 0.00% 20 31 0.00% 21 55 0.00% 22 52 0.00% 23 61 0.00% 24 81 0.00% 25 59 0.00% 26 77 0.00% 27 95 0.00% 28 79 0.00% 29 106 0.00% 30 96 0.00% 31 93 0.00% 32 91 0.00% 33 117 0.00% 34 87 0.00% 35 105 0.00% 36 98 0.00% 37 116 0.00% 38 127 0.00% 39 153 0.00% 40 181 0.00% 41 140 0.00% 42 134 0.00% 43 191 0.00% 44 167 0.00% 45 159 0.00% 46 196 0.00% 47 204 0.00% 48 196 0.00% 49 210 0.00% 50 234 0.00% 51 219 0.00% 52 268 0.00% 53 311 0.00% 54 300 0.00% 55 316 0.00% 56 315 0.00% 57 334 0.00% 58 410 0.00% 59 453 0.00% 60 468 0.00% 61 494 0.00% 62 582 0.00% 63 494 0.00% 64 590 0.00% 65 643 0.00% 66 601 0.00% 67 722 0.00% 68 706 0.00% 69 749 0.00% 70 836 0.00% 71 1013 0.00% 72 1178 0.00% 73 1227 0.00% 74 1260 0.00% 75 1406 0.00% 76 1489 0.00% 77 1576 0.00% 78 1664 0.00% 79 1956 0.00% 80 2119 0.00% 81 2471 0.00% 82 2676 0.00% 83 3049 0.00% 84 3624 0.01% 85 3875 0.01% 86 4128 0.01% 87 4359 0.01% 88 4855 0.01% 89 5266 0.01% 90 5820 0.01% 91 6417 0.01% 92 7219 0.01% 93 8279 0.01% 94 9354 0.01% 95 10357 0.02% 96 10935 0.02% 97 11844 0.02% 98 13522 0.02% 99 14522 0.02% 100 16050 0.02% 101 17537 0.03% 102 19322 0.03% 103 21733 0.03% 104 23958 0.04% 105 26215 0.04% 106 28676 0.04% 107 30382 0.05% 108 33195 0.05% 109 35814 0.05% 110 38307 0.06% 111 41795 0.06% 112 45619 0.07% 113 48732 0.07% 114 53839 0.08% 115 58442 0.09% 116 63854 0.10% 117 69438 0.10% 118 71339 0.11% 119 75193 0.11% 120 80211 0.12% 121 85130 0.13% 122 89881 0.13% 123 97198 0.15% 124 105425 0.16% 125 112065 0.17% 126 118426 0.18% 127 124363 0.19% 128 130140 0.19% 129 134327 0.20% 130 139788 0.21% 131 145487 0.22% 132 152687 0.23% 133 160472 0.24% 134 168041 0.25% 135 176952 0.26% 136 182908 0.27% 137 188126 0.28% 138 197138 0.29% 139 203828 0.30% 140 206906 0.31% 141 213426 0.32% 142 218362 0.33% 143 223719 0.33% 144 234178 0.35% 145 241843 0.36% 146 245394 0.37% 147 251992 0.38% 148 254530 0.38% 149 259342 0.39% 150 267735 0.40% 151 60591892 90.46% 66982472 reads passed initial QC criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=3.10 fanout-score-rank=28 prefix-density=0.30 prefix-fanout=2.4 sequence=CATCTCAGACCTCTCATAGAACATCTTAACTGGTGCAACACCTGCAATGATTGTCTCAGTTGTGGTGTTCTCTGAGAAACCTAAGTCAGGGTACATGCCACATTTGCAGCCACTGCCACACTTGCA criterion=fanout-score sequence-density=0.06 sequence-density-rank=32 fanout-score=222.59 fanout-score-rank=1 prefix-density=0.56 prefix-fanout=24.4 sequence=CCTTCTTCTCAACACTCTTAATGACACCAACCGCCACGGTCTGACGCATGTCCCTCACTGCAAAACGACCAAGAGGAGGATAGGCAGAAAAGGTCTCAACAACCATAGGCTTGGTGGGAATCATCTTCACAAACCCAGCATCACCATTCTTCAAGAACTTGGGCTCCTTCTCGAGCTCTTTGCCAGATCGCCTGTCAATCTTGGTCAAAATCTCAGCAAACTTGACAGCAATGTGGCAGGTGTGACAGTCAAGGACAGGGGCATATCCATTCCCAATTTGACCAGGGTGGTTCATGATGATGACCTGAGAGGTGAAGTTGGCAGCCTCCTTGGCAGGATCATCCTTAGAGTTGGAAGCAACAAAACCACGTTTGAGATCCTTGACAGCAACATTCTTAACATTGAAACCAACATTGTCACCTGGAAGGGCCTCCTGAAGAGCTTCATGGTGCATCTCAACAGATTTAACTTCAGTGGTCAGTCCAGTGGGGCCGAAGGTCA criterion=sequence-density sequence-density=0.23 sequence-density-rank=1 fanout-score=5.11 fanout-score-rank=26 prefix-density=0.58 prefix-fanout=2.1 sequence=CTGCAAGTGCGGCAGTGGCTG criterion=fanout-score sequence-density=0.10 sequence-density-rank=19 fanout-score=390.03 fanout-score-rank=1 prefix-density=1.13 prefix-fanout=33.0 sequence=AAGAAGAAGAAA SRR26075331 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 19:53:07 Started mapping on | Feb 11 19:53:08 Finished on | Feb 11 20:01:49 Mapping speed, Million of reads per hour | 462.83 Number of input reads | 66982472 Average input read length | 298 UNIQUE READS: Uniquely mapped reads number | 59938921 Uniquely mapped reads % | 89.48% Average mapped length | 297.03 Number of splices: Total | 59599201 Number of splices: Annotated (sjdb) | 58135830 Number of splices: GT/AG | 58526544 Number of splices: GC/AG | 832581 Number of splices: AT/AC | 60697 Number of splices: Non-canonical | 179379 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 3.38 Insertion rate per base | 0.02% Insertion average length | 2.62 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1597056 % of reads mapped to multiple loci | 2.38% Number of reads mapped to too many loci | 657703 % of reads mapped to too many loci | 0.98% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.42% % of reads unmapped: other | 0.73% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 5446495 5446495 5446495 N_multimapping 1597056 1597056 1597056 N_noFeature 1580069 59296044 1922122 N_ambiguous 632959 3954 329691 UnstrandedReadsAssigned:57725893 PositiveStrandReadsAssigned:638923 NegativeStrandReadsAssigned:57687108 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075331 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075331-trimmed-pair1.fastq SRR26075331-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 66,982,472 reads, 59,145,609 reads pseudoaligned [quant] estimated average fragment length: 224.556 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,147 rounds 52401 SRR26075331.ke.tsv 34699 SRR26075331.se.tsv 87100 total ==> SRR26075331.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1794.44 5775 42.9905 Potri.005G024800.1.v4.1 1035 811.444 5104 84.0238 Potri.004G059700.1.v4.1 961 737.467 2 0.0362275 Potri.007G009000.2.v4.1 1416 1192.44 0 0 Potri.003G141000.2.v4.1 2943 2719.44 2979.42 14.6353 Potri.016G087400.1.v4.1 270 80.0419 5254 876.846 Potri.015G069301.1.v4.1 564 342.382 0 0 Potri.010G195200.1.v4.1 1773 1549.44 1732.78 14.9389 Potri.012G127500.1.v4.1 977 753.444 86136 1527.16 ==> SRR26075331.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 208 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 582 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 6 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 538 SRR26075331 completed mapping pipeline successfully