Starting /dee2/code/volunteer_pipeline.sh SRR26075332 current disk space = 3053158322176 free memory = 1029437408 SRR26075332 SRAfilesize bba3b259990b6588b341367754b72d34 SRR26075332.sra SRR26075332.sra file validated SRR26075332 is paired end SRR26075332 is conventional basespace SRR26075332 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075332_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.47825 37.0 37.0 37.0 37.0 37.0 2 36.5635 37.0 37.0 37.0 37.0 37.0 3 36.608 37.0 37.0 37.0 37.0 37.0 4 36.627 37.0 37.0 37.0 37.0 37.0 5 36.715 37.0 37.0 37.0 37.0 37.0 6 36.642 37.0 37.0 37.0 37.0 37.0 7 36.5965 37.0 37.0 37.0 37.0 37.0 8 36.613 37.0 37.0 37.0 37.0 37.0 9 36.6185 37.0 37.0 37.0 37.0 37.0 10-14 36.62985 37.0 37.0 37.0 37.0 37.0 15-19 36.5723 37.0 37.0 37.0 37.0 37.0 20-24 36.484 37.0 37.0 37.0 37.0 37.0 25-29 36.3664 37.0 37.0 37.0 37.0 37.0 30-34 36.3614 37.0 37.0 37.0 37.0 37.0 35-39 36.285900000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.1608 37.0 37.0 37.0 37.0 37.0 45-49 35.21759999999999 37.0 37.0 37.0 29.8 37.0 50-54 34.865100000000005 37.0 37.0 37.0 27.4 37.0 55-59 34.4937 37.0 37.0 37.0 25.0 37.0 60-64 34.6209 37.0 37.0 37.0 25.0 37.0 65-69 34.4321 37.0 37.0 37.0 27.4 37.0 70-74 35.07790000000001 37.0 37.0 37.0 29.8 37.0 75-79 35.8378 37.0 37.0 37.0 37.0 37.0 80-84 35.837399999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.7525 37.0 37.0 37.0 37.0 37.0 90-94 35.740899999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.7507 37.0 37.0 37.0 37.0 37.0 100-104 35.7251 37.0 37.0 37.0 37.0 37.0 105-109 35.6476 37.0 37.0 37.0 37.0 37.0 110-114 35.542100000000005 37.0 37.0 37.0 37.0 37.0 115-119 35.4141 37.0 37.0 37.0 34.6 37.0 120-124 35.5025 37.0 37.0 37.0 37.0 37.0 125-129 35.3164 37.0 37.0 37.0 34.6 37.0 130-134 35.2675 37.0 37.0 37.0 32.2 37.0 135-139 35.19690000000001 37.0 37.0 37.0 32.2 37.0 140-144 35.105399999999996 37.0 37.0 37.0 29.8 37.0 145-149 35.1374 37.0 37.0 37.0 29.8 37.0 150-151 34.989999999999995 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 8.0 22 5.0 23 7.0 24 7.0 25 16.0 26 11.0 27 17.0 28 19.0 29 23.0 30 41.0 31 62.0 32 217.0 33 199.0 34 145.0 35 411.0 36 2648.0 37 162.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 45.82183186951067 12.923462986198246 8.105395232120452 33.14930991217064 2 16.825000000000003 20.474999999999998 32.625 30.075000000000003 3 16.525000000000002 17.525 35.175 30.775000000000002 4 20.625 22.5 24.55 32.324999999999996 5 29.65 27.275 24.525 18.55 6 28.125 31.624999999999996 22.3 17.95 7 16.075 31.15 37.425000000000004 15.35 8 16.7 30.599999999999998 30.125 22.575 9 23.525 23.400000000000002 32.45 20.625 10-14 19.875993799689983 30.65153257662883 26.02630131506575 23.44617230861543 15-19 20.565 27.04 27.560000000000002 24.834999999999997 20-24 19.955000000000002 28.53 28.285 23.23 25-29 19.785 28.215 26.889999999999997 25.11 30-34 19.2 28.38 27.965 24.455 35-39 19.75 28.78 27.639999999999997 23.830000000000002 40-44 18.87 27.36 27.32 26.450000000000003 45-49 21.375 26.33 28.355000000000004 23.94 50-54 22.755 25.95 27.18 24.115000000000002 55-59 23.615 26.305 26.915 23.165 60-64 24.565 25.06 27.83 22.545 65-69 22.98 27.155 27.894999999999996 21.97 70-74 26.200000000000003 25.374999999999996 25.145 23.28 75-79 26.064999999999998 26.1 25.28 22.555 80-84 25.759999999999998 26.035000000000004 26.150000000000002 22.055 85-89 25.840000000000003 25.83 25.330000000000002 23.0 90-94 26.205000000000002 26.105 25.290000000000003 22.400000000000002 95-99 26.895000000000003 24.87 25.46 22.775000000000002 100-104 26.375 24.955 25.96 22.71 105-109 26.484999999999996 25.505 25.705 22.305 110-114 26.005 25.275 25.230000000000004 23.49 115-119 26.76 25.235000000000003 25.180000000000003 22.825 120-124 26.779999999999998 24.335 25.95 22.935 125-129 27.075 26.155 24.240000000000002 22.53 130-134 28.235 25.900000000000002 23.945 21.92 135-139 27.439999999999998 25.385 24.635 22.54 140-144 27.560000000000002 25.455 25.5 21.485000000000003 145-149 27.150000000000002 25.88 24.3 22.67 150-151 28.6375 26.3 22.825 22.237499999999997 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.0 4 1.0 5 0.5 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 2.0 16 3.0 17 1.0 18 1.0 19 1.0 20 0.0 21 0.5 22 0.5 23 2.0 24 6.0 25 4.5 26 3.0 27 5.5 28 8.0 29 11.0 30 18.5 31 24.5 32 28.5 33 48.5 34 62.0 35 72.0 36 95.0 37 107.0 38 125.5 39 132.0 40 141.5 41 167.0 42 192.0 43 212.5 44 213.0 45 225.0 46 227.5 47 212.5 48 201.5 49 183.0 50 157.5 51 129.5 52 130.0 53 124.0 54 97.5 55 72.0 56 52.0 57 53.0 58 43.0 59 31.5 60 23.5 61 11.0 62 10.0 63 15.5 64 13.0 65 7.0 66 9.5 67 15.5 68 19.0 69 25.0 70 35.0 71 39.0 72 39.5 73 40.5 74 29.0 75 17.5 76 12.0 77 5.0 78 0.5 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.8 #Duplication Level Percentage of deduplicated Percentage of total 1 67.35197368421053 40.949999999999996 2 19.407894736842106 23.599999999999998 3 7.8125 14.249999999999998 4 2.5904605263157894 6.3 5 1.3157894736842104 4.0 6 0.8223684210526315 3.0 7 0.20559210526315788 0.8750000000000001 8 0.12335526315789473 0.6 9 0.08223684210526315 0.44999999999999996 >10 0.24671052631578946 4.075 >50 0.041118421052631575 1.9 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTACACCATCTCGGTT 76 1.9 TruSeq Adapter, Index 22 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTACACCATCTCGTTT 44 1.0999999999999999 TruSeq Adapter, Index 22 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTACACCATCTCGGGT 39 0.975 TruSeq Adapter, Index 22 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTACACCATCGCGGTT 31 0.775 TruSeq Adapter, Index 22 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTACACCATCGCGGGT 23 0.575 TruSeq Adapter, Index 22 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTACACCATCTCGTGT 16 0.4 TruSeq Adapter, Index 22 (97% over 38bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTACACCATCTCGTAT 10 0.25 TruSeq Adapter, Index 22 (97% over 38bp) GTTGAGGAGAAGGGCTCGATGGAAGATGCATTAGCGACTTCAGCTGGATC 9 0.22499999999999998 No Hit GGCAGGAAAAGTGACATCAAAATTGATGCGTAGGCTTTGCCCTCTTGAAG 9 0.22499999999999998 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTACACCATCGCGTTT 8 0.2 TruSeq Adapter, Index 22 (97% over 38bp) GCCCTAGTTTGGTACGTCGCCAGTGCCTGCGCTTCGCATTGTACCTGATG 8 0.2 No Hit GTTTTGTCTTCAAAGATGCCTGGTGTTTAGTAAGACGTCTGCGAATGGCA 8 0.2 No Hit AGGGAATGTTTGATTATTACCCAATCAGACTAGAGACTAAGAATTTGAGG 7 0.17500000000000002 No Hit CGTGACGCGACCAAACCCATAGCCACCACCATCTAGTAACAGAACCATAT 7 0.17500000000000002 No Hit GTCCACGGTATCATCAATCTCTTCAAAGCAATCCTTGATCGCTGCCTTTT 7 0.17500000000000002 No Hit GCTCTTCGTAGGTGCTCCTTCTTAACACGTTTCAGAACCTCTCCTATGTG 7 0.17500000000000002 No Hit CTCCCAACTGATTCATCAGCAACAACGAGAAAAACAAATCCATTATCAAT 7 0.17500000000000002 No Hit CTTACAAGCATGCCTCAAAGGTGGCGGTGCCATCCTCTTCTTTATCAAGC 6 0.15 No Hit CCTGTACTTCTTTCCAGCAGATGTTAGACCACGTAGCTCTCTGTGTTTAT 6 0.15 No Hit CTTTCTTCGTGTATGTTTCCTCGTTGAAATTGCTTGTGAAGAACCTATCT 6 0.15 No Hit GCTAACAATCAACTCCACATTTGACTGTCATTAATCATAACAAAATTTTG 6 0.15 No Hit CAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACA 6 0.15 No Hit CTGGCGGTCACATCTCACAGTGATACAATTGCCTACCCACTCAAAATGTC 6 0.15 No Hit GTGAGAGCCAGGGGGTTGAAATTGGATGTGCGGGCAGTGCCTTTCAGGTT 6 0.15 No Hit GTACTGCCAAATCCTGATAGAGCCCGCAGTCTCCCAGTCACCTGCATGTA 6 0.15 No Hit AGCTAACATCCTGGGTATCAACCTTAACATCCCACTCTCTCTAAGCTTGC 6 0.15 No Hit CACACAATGAAGAAATATTAAAGAATTGAAGTGAATTTGGGTGTGGATTA 6 0.15 No Hit GTCTGGTTAACTTAACATCTTCCAAGGCATGTTCTGCTTGCGTCTTCTGG 6 0.15 No Hit GCAAGTGTACATTCACCGTTGTTTGTGAATGAAGGTATTAGGGATTCAGC 6 0.15 No Hit ACCTTCCGAAATATTATCTTCAAAAAGCAGATTCAGTGGCCTAACCCCTT 6 0.15 No Hit CTCTGCCTGTACTGCTTTATCCTCTTCACATTCTGTTCATTCCCCTTGGC 6 0.15 No Hit GCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAA 6 0.15 No Hit ATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCACTTCCAAGCAT 6 0.15 No Hit GATAGAGCTCGCAACGGCAGAATTTCCAATATTTGCATTAACTTTAACCA 6 0.15 No Hit CAACCCTACTTATCAACCCTGTCTTGATCACACAACTTCAGAATTTGGTG 6 0.15 No Hit GTCAAATGTAAGGTTCATCGGAAAACAACCACGGAATTGAATTCAAACTT 6 0.15 No Hit CCTCTACCACGACCAGCATAGCCATAACCTCTTCCATAATAACCACCATG 6 0.15 No Hit CTGGGATAGATGCCGGTCGGGAAATCTCCAATGACACCTGTAGGTCGACG 5 0.125 No Hit GGCTCGAGATGATTCTGTGTTAGACATGTAGTTGGGGTAACCCGGGTATC 5 0.125 No Hit CATTTTCACAGCCTGTGAAGCCACAGCAGAAAGCAACATCTTCCCTAGTC 5 0.125 No Hit GATTGATGAAGGACCAGGAGAACTGGAATCGGCTTTAGCATAATCTGATG 5 0.125 No Hit CCAACTTCCCACCCTCATACTCTGATTCTGGTATTCTAGCATTCACCCCA 5 0.125 No Hit CATTAATCTATCTCTGTCATCCTGGATAAAATTTCACAATTACACTCCAA 5 0.125 No Hit GTTAGTTACTGATCAGTAGAATTTGTTAAAGCCATTCCGATTGCAGTCCT 5 0.125 No Hit GGCAGAAACTGATACATTCCAGTCCCTGGGGTATCAAACTATGCAAAATG 5 0.125 No Hit ATGGCTGATTATATTTAGCTCCTAAAGGAGAAACCATGTCCTCTGGGCTT 5 0.125 No Hit CCTGCAACCTCTACTGTGGCCTAACCCTATGGCTCAACACCATTTCCCTA 5 0.125 No Hit GTATCATTGTAGCTATGCATTATCGCTGCCCAACGATCATTTAGAAGCTG 5 0.125 No Hit GCGGTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAA 5 0.125 No Hit GTACAGTTCATAGTCCATCACAAATTGCTGCGACATGGTTGGCAAGAATC 5 0.125 No Hit GCCTTCTCTGCCTTTGATTTGTGGATGCTTTCCATTAGAACTCTCTTGTT 5 0.125 No Hit CAGCAACGCCACACATATGAAAAACAAGTAAGAGACTGGCCATGACATGG 5 0.125 No Hit GTGTAAGCTGCCAGACGGGCCTATAGACCTTGTAAATTTTCATGACATCT 5 0.125 No Hit ATGCATGAGAATACAATTATTGCCACAAACAAGATACAGCAATATAAAGT 5 0.125 No Hit GCCGAATGATACACTAGCATTTTTCTCTTCTTGGCCTCTTATGAGCTTGT 5 0.125 No Hit CGCCGATCAAGAACCCAATTCTGCGAACAGCCTCTTGATGAGAGTAGTGA 5 0.125 No Hit TCACATGAGCAGCTCGGAATGATTCCTTTGTTCTTCCATAGTAGATTGGC 5 0.125 No Hit TTCCTCAGCCTTCTGTTTCTCCTTCTCAGCATCAAACTTTGGCTTCCATG 5 0.125 No Hit ATATACCAAGTGCAACTTAGTATGACCAGCACCAAAAGGCATGATCTTCA 5 0.125 No Hit CTCTTCTTCAAGTGACGGCCCTCATTCAGCTGAGCAATAAAACCATTGCG 5 0.125 No Hit GGGGTCTCGCCTTCGGATCATGGATATACGGTGTTGATAGAGTACATAAC 5 0.125 No Hit ACTTGTCTCCACCTGCTTTACCAACAGCAGCAACAGATTTATTGTTTGGA 5 0.125 No Hit ATTTGATTTTATACAAATGCTCAATGAAAAACTGATATCAGAAGAAGAAG 5 0.125 No Hit AGGAAGAAATATGACAAGGAGTAGTAGTGTGGATGTTGTTGTTAGACACT 5 0.125 No Hit TCTTGATTGTGCTGCTCGGCACTTTGCACATGGGTGGTGACTGGATTGGC 5 0.125 No Hit CAGCAGCATTCATAGCATTTGGAAGATCTTGCTTGTTTGCAAACACAAGT 5 0.125 No Hit TATCTCTACATTTTCAAACTATACGTTCGAGTTGCTACAAGTATCAACAT 5 0.125 No Hit GGCAGATGGCATGGGTTCTTGCTCCATGTCAGCGTAATTAATCTTAGGAT 5 0.125 No Hit GTTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAACTAACACCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.037500000000000006 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.1375 0.0 0.0 0.0 0.0 64-65 0.15 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.15 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.21250000000000002 0.0 0.0 0.0 0.0 90-91 0.25 0.0 0.0 0.0 0.0 92-93 0.3 0.0 0.0 0.0 0.0 94-95 0.4375 0.0 0.0 0.0 0.0 96-97 0.5125 0.0 0.0 0.0 0.0 98-99 0.6 0.0 0.0 0.0 0.0 100-101 0.65 0.0 0.0 0.0 0.0 102-103 0.7375 0.0 0.0 0.0 0.0 104-105 0.7625 0.0 0.0 0.0 0.0 106-107 0.85 0.0 0.0 0.0 0.0 108-109 0.9624999999999999 0.0 0.0 0.0 0.0 110-111 1.0750000000000002 0.0 0.0 0.0 0.0 112-113 1.1375 0.0 0.0 0.0 0.0 114-115 1.2374999999999998 0.0 0.0 0.0 0.0 116-117 1.5499999999999998 0.0 0.0 0.0 0.0 118-119 1.95 0.0 0.0 0.0 0.0 120-121 2.2750000000000004 0.0 0.0 0.0 0.0 122-123 2.5625 0.0 0.0 0.0 0.0 124-125 2.9875 0.0 0.0 0.0 0.0 126-127 3.2750000000000004 0.0 0.0 0.0 0.0 128-129 3.4875 0.0 0.0 0.0 0.0 130-131 3.825 0.0 0.0 0.0 0.0 132-133 4.4125 0.0 0.0 0.0 0.0 134-135 5.1 0.0 0.0 0.0 0.0 136-137 5.4125 0.0 0.0 0.0 0.0 138-139 6.15 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCATTC 10 0.006830828 145.0 2 GAGCACA 50 1.364242E-10 101.5 9 AGAGCAC 55 2.928573E-10 92.272736 8 GAAGAGC 60 5.838956E-10 84.583336 6 GGAAGAG 60 5.838956E-10 84.583336 5 CGGAAGA 55 3.3778633E-8 79.09091 4 AAGAGCA 70 1.9790605E-9 72.50001 7 TCGGAAG 60 6.176924E-8 72.5 3 GATCGGA 65 1.0757503E-7 66.92307 1 ATCGGAA 65 1.0757503E-7 66.92307 2 TTGGCGG 20 0.00593511 29.0 45-49 TGGCGGG 25 4.977651E-4 29.0 50-54 CAGTCAC 40 9.990927E-6 25.375 25-29 CCAGTCA 40 9.990927E-6 25.375 25-29 CACTGTA 40 9.990927E-6 25.375 30-34 TACACCA 40 9.990927E-6 25.375 35-39 GAACTCC 40 9.990927E-6 25.375 20-24 TCACTGT 40 9.990927E-6 25.375 30-34 CTGTACA 40 9.990927E-6 25.375 30-34 AGGGGGG 30 0.0014437955 24.166668 65-69 >>END_MODULE SRR26075332 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075332_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1415 37.0 37.0 37.0 37.0 37.0 2 36.136 37.0 37.0 37.0 37.0 37.0 3 36.1155 37.0 37.0 37.0 37.0 37.0 4 36.035 37.0 37.0 37.0 37.0 37.0 5 36.183 37.0 37.0 37.0 37.0 37.0 6 36.0085 37.0 37.0 37.0 37.0 37.0 7 35.9665 37.0 37.0 37.0 37.0 37.0 8 35.8675 37.0 37.0 37.0 37.0 37.0 9 35.9375 37.0 37.0 37.0 37.0 37.0 10-14 35.7269 37.0 37.0 37.0 37.0 37.0 15-19 35.5892 37.0 37.0 37.0 37.0 37.0 20-24 35.3053 37.0 37.0 37.0 34.6 37.0 25-29 34.6779 37.0 37.0 37.0 25.0 37.0 30-34 34.3709 37.0 37.0 37.0 25.0 37.0 35-39 34.0456 37.0 37.0 37.0 25.0 37.0 40-44 33.8559 37.0 37.0 37.0 19.4 37.0 45-49 33.7036 37.0 37.0 37.0 13.8 37.0 50-54 33.399800000000006 37.0 37.0 37.0 11.0 37.0 55-59 33.5592 37.0 37.0 37.0 13.8 37.0 60-64 33.904 37.0 37.0 37.0 25.0 37.0 65-69 33.6284 37.0 37.0 37.0 13.8 37.0 70-74 33.420100000000005 37.0 37.0 37.0 11.0 37.0 75-79 33.2744 37.0 37.0 37.0 11.0 37.0 80-84 33.43589999999999 37.0 37.0 37.0 11.0 37.0 85-89 33.7548 37.0 37.0 37.0 19.4 37.0 90-94 33.9739 37.0 37.0 37.0 25.0 37.0 95-99 34.3801 37.0 37.0 37.0 25.0 37.0 100-104 34.44449999999999 37.0 37.0 37.0 25.0 37.0 105-109 34.551 37.0 37.0 37.0 25.0 37.0 110-114 34.5132 37.0 37.0 37.0 25.0 37.0 115-119 34.5383 37.0 37.0 37.0 25.0 37.0 120-124 34.5129 37.0 37.0 37.0 25.0 37.0 125-129 34.5135 37.0 37.0 37.0 25.0 37.0 130-134 34.5031 37.0 37.0 37.0 25.0 37.0 135-139 34.422399999999996 37.0 37.0 37.0 25.0 37.0 140-144 34.4715 37.0 37.0 37.0 25.0 37.0 145-149 34.4288 37.0 37.0 37.0 25.0 37.0 150-151 34.146 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 7.0 14 20.0 15 28.0 16 28.0 17 16.0 18 30.0 19 16.0 20 17.0 21 29.0 22 28.0 23 35.0 24 50.0 25 52.0 26 60.0 27 59.0 28 47.0 29 28.0 30 18.0 31 31.0 32 39.0 33 80.0 34 160.0 35 561.0 36 2374.0 37 186.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.175000000000004 19.25 10.549999999999999 18.025 2 37.325 22.825 21.875 17.974999999999998 3 31.900000000000002 24.224999999999998 26.400000000000002 17.474999999999998 4 33.575 27.3 20.95 18.175 5 34.625 30.8 18.275 16.3 6 30.8 32.175 19.275000000000002 17.75 7 32.225 19.075 31.1 17.599999999999998 8 30.95 23.65 23.225 22.175 9 29.4 23.925 25.45 21.224999999999998 10-14 32.395 25.305 22.720000000000002 19.580000000000002 15-19 32.655 25.740000000000002 22.49 19.115 20-24 31.830000000000002 26.21 22.82 19.139999999999997 25-29 32.48 26.19 23.044999999999998 18.285 30-34 31.75 25.46 24.279999999999998 18.509999999999998 35-39 31.724999999999998 25.22 23.74 19.314999999999998 40-44 30.990000000000002 26.375 23.685000000000002 18.95 45-49 30.845 25.564999999999998 24.34 19.25 50-54 23.25 26.365 30.44 19.945 55-59 30.020000000000003 26.185000000000002 24.865000000000002 18.93 60-64 31.35 25.790000000000003 24.2 18.66 65-69 31.885 25.53 23.76 18.825 70-74 26.779999999999998 30.014999999999997 24.675 18.529999999999998 75-79 25.009999999999998 31.674999999999997 24.490000000000002 18.825 80-84 29.565 26.35 24.955 19.13 85-89 30.675 26.424999999999997 24.135 18.765 90-94 30.03 26.765 24.325 18.88 95-99 30.56 26.669999999999998 23.89 18.88 100-104 30.475 26.445 24.445 18.634999999999998 105-109 30.255 26.665 24.285 18.795 110-114 30.240000000000002 26.915 23.990000000000002 18.855 115-119 30.29 26.655 24.42 18.634999999999998 120-124 30.605 27.685 23.544999999999998 18.165 125-129 31.1 27.474999999999998 24.04 17.385 130-134 30.755 27.389999999999997 24.195 17.66 135-139 31.275 26.32 24.6 17.805 140-144 31.115 27.765 23.724999999999998 17.395 145-149 31.225 27.060000000000002 24.14 17.575 150-151 32.087500000000006 26.2125 24.55 17.150000000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 1.0 7 1.5 8 0.5 9 2.5 10 4.0 11 2.0 12 2.0 13 1.5 14 1.0 15 1.5 16 2.0 17 3.0 18 3.5 19 3.5 20 3.0 21 2.0 22 0.5 23 1.0 24 3.5 25 3.0 26 3.0 27 4.5 28 4.5 29 5.0 30 6.0 31 11.0 32 11.5 33 14.0 34 20.0 35 29.0 36 48.5 37 64.5 38 87.0 39 118.5 40 164.5 41 191.5 42 193.5 43 197.0 44 215.0 45 266.5 46 267.5 47 244.5 48 235.0 49 186.0 50 165.0 51 153.0 52 121.5 53 105.0 54 95.0 55 76.5 56 53.5 57 41.0 58 34.0 59 29.0 60 21.5 61 18.5 62 18.0 63 14.5 64 10.0 65 7.5 66 4.0 67 3.0 68 5.5 69 5.0 70 3.0 71 3.0 72 4.5 73 5.0 74 4.0 75 3.0 76 4.5 77 6.0 78 7.0 79 6.5 80 6.5 81 12.5 82 16.5 83 16.0 84 16.0 85 20.0 86 22.5 87 21.0 88 25.0 89 23.5 90 18.0 91 20.5 92 19.0 93 16.0 94 14.0 95 10.0 96 10.5 97 10.5 98 5.5 99 3.5 100 18.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.125 #Duplication Level Percentage of deduplicated Percentage of total 1 69.61770623742454 43.25 2 18.390342052313883 22.85 3 6.881287726358148 12.825000000000001 4 2.414486921529175 6.0 5 1.2877263581488934 4.0 6 0.7243460764587526 2.7 7 0.2012072434607646 0.8750000000000001 8 0.2012072434607646 1.0 9 0.16096579476861167 0.8999999999999999 >10 0.04024144869215292 0.3 >50 0.04024144869215292 2.3 >100 0.04024144869215292 3.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 120 3.0 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 92 2.3 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT 12 0.3 No Hit GTGCCCTCCACCGACGTTGCTGCCACTTCCACCATCCCACCACTCACCAA 9 0.22499999999999998 No Hit GGCTGCTTCAGTCTCTTGCCTCCAAGCCCCCAACTCGGGAAAATGCCGTC 9 0.22499999999999998 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGT 9 0.22499999999999998 No Hit CTCTCCGGAGGCCTTATCACTCTCATCTCTTCCGTTCTCATTCTTTTCCT 9 0.22499999999999998 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGT 8 0.2 No Hit CAGAAAGCACCACAACTGAGACAATCATTGCAGGTTTAGCACCAGTTCAC 8 0.2 No Hit GCTCGCTCTCCTCCGTGTTGCCAAGGTTACTGGCGGTGCCCCTAACAAGC 8 0.2 No Hit GCTAGATACTAGTAGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGTTC 8 0.2 No Hit ATCTCCTCAAACAAACCCTAGAAACTCTCTCTCCAAGAAATCATCATGAA 8 0.2 No Hit GCCAGCTAATTCAGACGGCCTTGGCTGTGAGCCTAGACAAAGCTCAATCC 7 0.17500000000000002 No Hit AAGGATTGCTTACTGGAGGAACCACATTGTTGTAACAATACTTTCTGCTT 7 0.17500000000000002 No Hit TGGTGCTAGCAGAGCACACATCGTATTCGGGGAACTTTAGTACCATTGCT 7 0.17500000000000002 No Hit CAGTTACTGGGTATTACAGGCCTGCTAATTATGTAGCAGAGATTGATCCA 7 0.17500000000000002 No Hit GGTTGCTCCTATCCCAGGGGAAACTAAGGTCTGGCAGTATATAACACTCA 7 0.17500000000000002 No Hit GGTGGAGTCTTATCTGGAAGTGCTGTTAGGGAGAGAATTATCCGGGCTTT 6 0.15 No Hit GGCAGAGGTTTTCGGAGAAAATTATTGTGCTGCTTCTTACTGTTGATGCC 6 0.15 No Hit AGCTAGCTGGGTTTGCATGTACTCGGATACGGGAGTCGTGTTTCATGTGT 6 0.15 No Hit GATGTAACGGATGATCAGGAGGTGATAGAAGCTCTTTATGAAGTCTATGG 6 0.15 No Hit GGATTCCACGTACAAATATTTTGAGGTTATCCTGGTCGATGCTGCTCACA 6 0.15 No Hit GGCTATAGCATAATTGGGTTTGGTGACATCATCTTACCAGGATTGCTTGT 6 0.15 No Hit TGATGAAGCAGTTTCAAGGACAGCTGAGAATAGCCCTCGTTTGTTTTCTG 6 0.15 No Hit GTGAAAAGCTTGACCCGGAGTTTGTGAGGTCAGAGGTTGCTCGTGGGAGG 6 0.15 No Hit TGCTACTAAGAATATTGGTGCTCATGTTTTTGACAAGCCACAGCCTAACA 6 0.15 No Hit CACACAATTCTTAATAGAAAACATACTTCAAAGCATGCAAAGGCGCCGTA 6 0.15 No Hit TGCATCAAACTGTCCCCTACTCTCAGGCCTAGCATGTCCGACGTTGTTAC 6 0.15 No Hit GTTTACTCGGCGAGACTTGCAGAGCAAGCTGAGAGATACGATGAGATGGT 6 0.15 No Hit GTTCTGTTGCATGATGAAACTCACTTTTATGAATTGTAGTAACAACATTT 6 0.15 No Hit ATTTCATCTTACCCAACCCCTTATGAAGATGTCAATTTAAACGCTATTAA 6 0.15 No Hit GGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTC 6 0.15 No Hit GGGTCAAGGACCAGTGCTATGACAGGGAAAATGGAAGATGGGGCGGTGGC 6 0.15 No Hit CAGCACTAACCAGCAGAAACCAGCAAACAATTCTAAAGCTGTTTTGATTT 6 0.15 No Hit ACTACAGCTAATTTCGCCTCCTTCTAAATCAATACCTGTCGCCTTTCAAT 6 0.15 No Hit GAAGAGAAATCCAGCATACAAGGGAAAATGGCATGCTCCACTTATTGACA 5 0.125 No Hit AACGAACGAACATGGGTCTCATGGCTAAACATCGCGGTTCTTCTGGCAAC 5 0.125 No Hit CAAATCTGCTGCCAAGAAGGGTGGCAAGTGAACGGAGCAGGTTGATGCTA 5 0.125 No Hit GTTTCAAGCATAAAGTCTGATGGAAGTAGCAAAGGCAGACCTCCCCGGAA 5 0.125 No Hit CAGGATTTGGCAGTACACAATCGGAGGGAAAGCCGGGGTCTTTAAAGAGG 5 0.125 No Hit CCAGAAGCTGAAACATTCAGAAATGGGATCAATGGAGATGCTGTGGTTGC 5 0.125 No Hit CAGAGAGCTAGTGTTAGACAGGTTCTTGTTAGACAGAGAGATGGAGAAGA 5 0.125 No Hit CAACAACATTACACACTTTAAGGAGTTTACAAGTCTCGAAGAGAACCAAC 5 0.125 No Hit AATAATATCCCGGATCATCATGGTGATGCAGGGAGCAGCAGTCTCCTCAA 5 0.125 No Hit GTGAGGAGATAGCAAGGAGACTCATGTTTAAGAGAGAGGATGATTGACTC 5 0.125 No Hit ATGTGTTTGAACATGACAAGAGTGAGCCTCCAGTTGCCTTCCTCAATTCG 5 0.125 No Hit AGAAAGTGCAATAGAGACCCTTCAAACTATTCCTGACATAGATGATGAGC 5 0.125 No Hit GATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCAT 5 0.125 No Hit AGTTGCCTTGCCTCACATTGAGGAAGATGTTCAACAAGAGACATCTGGTG 5 0.125 No Hit TATAGAGATGAAAGCGGGGAGATAATCTGTGAAGGATTCGATGAAGGCCC 5 0.125 No Hit AGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGG 5 0.125 No Hit CTTCGAGCCCGACCAATCGGATTTATCACAAGATGAAGAAGATAACTTGA 5 0.125 No Hit AATCAAGGTGCAAATCACTGGGGTGAGGGTCCATCATCAAATTTTCATGC 5 0.125 No Hit GTCCTCAAACCATTAAACAGTGATGATAGTGAAGTTAGTGGGTCAGATGA 5 0.125 No Hit GCGTCATGATGTCCTAGACAGACTCCCTTTTAATATTGGAATCTCGTTGT 5 0.125 No Hit GCTCTTGATGGTGGATCACGCAATGGGAGGGCTAGGTTTTCCCAGCAGTC 5 0.125 No Hit GGTAAGCGCAAGGGCACCAGGGAAGCTAGGCTGCCAACAAAGGTCCTCTG 5 0.125 No Hit GGAGCAGGAAAGGTAACCTAGAGGAGGAGTTGTAATATGTATAGTTAAAA 5 0.125 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 5 0.125 No Hit GGACAAGATCCGACCATTGTGGAGGCATTATTTCCAAAACACTCAAGGGC 5 0.125 No Hit ACTGGCGATGCGGGATGAACCGAACGCGAGGTTAAGGTGCCGGAATTCAC 5 0.125 No Hit AAAGCCCCAGGAGCAGCTTCATGACACAGCAGATCAGACCCGACCAGGCC 5 0.125 No Hit TAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGG 5 0.125 No Hit CACGACCTCAATTTCGGATATACTCATGCTTTTGAAACTACCTTTGATGA 5 0.125 No Hit GTAGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGTTCTTGTTAGACAG 5 0.125 No Hit TACTGATATTGGTGATGGTATTGGTGATGGTGAAGGTTCATCAAAATCTA 5 0.125 No Hit GAGGAGGTTATCGACAAGGTAGTGAAAGAGGCAATCAACAAGCCATGGCT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.037500000000000006 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.0625 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.1 0.0 0.0 0.0 0.0 84-85 0.125 0.0 0.0 0.0 0.0 86-87 0.15 0.0 0.0 0.0 0.0 88-89 0.16249999999999998 0.0 0.0 0.0 0.0 90-91 0.2 0.0 0.0 0.0 0.0 92-93 0.25 0.0 0.0 0.0 0.0 94-95 0.3875 0.0 0.0 0.0 0.0 96-97 0.4625 0.0 0.0 0.0 0.0 98-99 0.55 0.0 0.0 0.0 0.0 100-101 0.6 0.0 0.0 0.0 0.0 102-103 0.6875 0.0 0.0 0.0 0.0 104-105 0.7124999999999999 0.0 0.0 0.0 0.0 106-107 0.8 0.0 0.0 0.0 0.0 108-109 0.9125000000000001 0.0 0.0 0.0 0.0 110-111 1.0375 0.0 0.0 0.0 0.0 112-113 1.1125 0.0 0.0 0.0 0.0 114-115 1.2125 0.0 0.0 0.0 0.0 116-117 1.5375 0.0 0.0 0.0 0.0 118-119 1.95 0.0 0.0 0.0 0.0 120-121 2.2750000000000004 0.0 0.0 0.0 0.0 122-123 2.5625 0.0 0.0 0.0 0.0 124-125 3.0125 0.0 0.0 0.0 0.0 126-127 3.3 0.0 0.0 0.0 0.0 128-129 3.5625 0.0 0.0 0.0 0.0 130-131 3.9 0.0 0.0 0.0 0.0 132-133 4.4875 0.0 0.0 0.0 0.0 134-135 5.175000000000001 0.0 0.0 0.0 0.0 136-137 5.4875 0.0 0.0 0.0 0.0 138-139 6.2625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCAAAA 10 0.006830828 145.0 1 AAGTCCT 10 0.006830828 145.0 6 >>END_MODULE Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825488 spots for SRR26075332.sra Written 2825488 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra Read 2825473 spots for SRR26075332.sra Written 2825473 spots for SRR26075332.sra SRR ids: ['SRR26075332.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_o33l5uu0 SRR26075332.sra spots: 56509475 blocks: [[1, 2825473], [2825474, 5650946], [5650947, 8476419], [8476420, 11301892], [11301893, 14127365], [14127366, 16952838], [16952839, 19778311], [19778312, 22603784], [22603785, 25429257], [25429258, 28254730], [28254731, 31080203], [31080204, 33905676], [33905677, 36731149], [36731150, 39556622], [39556623, 42382095], [42382096, 45207568], [45207569, 48033041], [48033042, 50858514], [50858515, 53683987], [53683988, 56509475]] SRR26075332 file size 20874764 SRR26075332 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075332 SRR26075332_1.fastq SRR26075332_2.fastq Input file: SRR26075332_1.fastq Paired file: SRR26075332_2.fastq trimmed: SRR26075332-trimmed-pair1.fastq, SRR26075332-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 20:23:51 2025 >> started Tue Feb 11 20:25:00 2025 >> done (68.267s) 56509475 read pairs processed; of these: 2859 ( 0.01%) short read pairs filtered out after trimming by size control 3140359 ( 5.56%) empty read pairs filtered out after trimming by size control 53366257 (94.44%) read pairs available; of these: 6703335 (12.56%) trimmed read pairs available after processing 46662922 (87.44%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 45 0.00% 19 49 0.00% 20 30 0.00% 21 59 0.00% 22 61 0.00% 23 83 0.00% 24 76 0.00% 25 81 0.00% 26 108 0.00% 27 98 0.00% 28 137 0.00% 29 137 0.00% 30 149 0.00% 31 167 0.00% 32 134 0.00% 33 129 0.00% 34 204 0.00% 35 204 0.00% 36 213 0.00% 37 250 0.00% 38 333 0.00% 39 273 0.00% 40 439 0.00% 41 356 0.00% 42 338 0.00% 43 374 0.00% 44 392 0.00% 45 466 0.00% 46 488 0.00% 47 466 0.00% 48 594 0.00% 49 599 0.00% 50 684 0.00% 51 750 0.00% 52 1092 0.00% 53 817 0.00% 54 1042 0.00% 55 1680 0.00% 56 939 0.00% 57 1450 0.00% 58 2397 0.00% 59 1323 0.00% 60 2181 0.00% 61 1429 0.00% 62 1585 0.00% 63 2703 0.01% 64 1811 0.00% 65 1942 0.00% 66 1847 0.00% 67 1817 0.00% 68 1896 0.00% 69 2103 0.00% 70 2220 0.00% 71 2405 0.00% 72 2453 0.00% 73 2831 0.01% 74 3116 0.01% 75 3159 0.01% 76 3369 0.01% 77 3668 0.01% 78 3803 0.01% 79 4023 0.01% 80 4280 0.01% 81 4961 0.01% 82 5543 0.01% 83 5917 0.01% 84 6367 0.01% 85 6697 0.01% 86 7034 0.01% 87 7547 0.01% 88 7890 0.01% 89 9062 0.02% 90 9476 0.02% 91 10329 0.02% 92 11788 0.02% 93 12316 0.02% 94 14014 0.03% 95 14925 0.03% 96 15945 0.03% 97 17155 0.03% 98 18305 0.03% 99 18932 0.04% 100 20367 0.04% 101 22185 0.04% 102 24300 0.05% 103 27470 0.05% 104 29865 0.06% 105 31694 0.06% 106 33994 0.06% 107 36083 0.07% 108 38263 0.07% 109 40878 0.08% 110 43864 0.08% 111 45812 0.09% 112 51555 0.10% 113 55359 0.10% 114 60020 0.11% 115 64419 0.12% 116 68056 0.13% 117 73015 0.14% 118 76076 0.14% 119 80637 0.15% 120 83630 0.16% 121 88531 0.17% 122 94665 0.18% 123 101390 0.19% 124 109973 0.21% 125 114208 0.21% 126 121944 0.23% 127 128718 0.24% 128 134120 0.25% 129 138775 0.26% 130 142943 0.27% 131 147748 0.28% 132 157000 0.29% 133 164726 0.31% 134 172507 0.32% 135 179382 0.34% 136 187204 0.35% 137 192434 0.36% 138 198967 0.37% 139 203424 0.38% 140 208412 0.39% 141 213203 0.40% 142 220724 0.41% 143 225844 0.42% 144 233336 0.44% 145 243216 0.46% 146 248591 0.47% 147 254964 0.48% 148 260107 0.49% 149 262296 0.49% 150 267891 0.50% 151 46662922 87.44% 53366257 reads passed initial QC criterion=sequence-density sequence-density=0.60 sequence-density-rank=1 fanout-score=25.88 fanout-score-rank=4 prefix-density=0.55 prefix-fanout=25.9 sequence=GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTACACCATCTCGT criterion=fanout-score sequence-density=0.03 sequence-density-rank=34 fanout-score=90.39 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=10.0 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.39 sequence-density-rank=1 fanout-score=2.53 fanout-score-rank=29 prefix-density=0.39 prefix-fanout=2.5 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=90.46 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=4.7 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTA SRR26075332 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 20:26:50 Started mapping on | Feb 11 20:26:50 Finished on | Feb 11 20:43:05 Mapping speed, Million of reads per hour | 197.04 Number of input reads | 53366257 Average input read length | 289 UNIQUE READS: Uniquely mapped reads number | 36586038 Uniquely mapped reads % | 68.56% Average mapped length | 287.94 Number of splices: Total | 33585319 Number of splices: Annotated (sjdb) | 32730853 Number of splices: GT/AG | 32942193 Number of splices: GC/AG | 494180 Number of splices: AT/AC | 33120 Number of splices: Non-canonical | 115826 Mismatch rate per base, % | 0.44% Deletion rate per base | 0.04% Deletion average length | 3.17 Insertion rate per base | 0.02% Insertion average length | 2.53 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1242064 % of reads mapped to multiple loci | 2.33% Number of reads mapped to too many loci | 280117 % of reads mapped to too many loci | 0.52% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 27.16% % of reads unmapped: other | 1.43% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 15538156 15538156 15538156 N_multimapping 1242064 1242064 1242064 N_noFeature 936543 36023908 1251306 N_ambiguous 725888 10511 470051 UnstrandedReadsAssigned:34923607 PositiveStrandReadsAssigned:551619 NegativeStrandReadsAssigned:34864681 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075332 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075332-trimmed-pair1.fastq SRR26075332-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 53,366,257 reads, 40,968,387 reads pseudoaligned [quant] estimated average fragment length: 200.488 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,278 rounds 52401 SRR26075332.ke.tsv 34699 SRR26075332.se.tsv 87100 total ==> SRR26075332.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1818.51 3165 31.4747 Potri.005G024800.1.v4.1 1035 835.512 2420 52.3802 Potri.004G059700.1.v4.1 961 761.512 19 0.451213 Potri.007G009000.2.v4.1 1416 1216.51 0 0 Potri.003G141000.2.v4.1 2943 2743.51 1586.17 10.4556 Potri.016G087400.1.v4.1 270 90.8427 6105.08 1215.36 Potri.015G069301.1.v4.1 564 364.926 0 0 Potri.010G195200.1.v4.1 1773 1573.51 464 5.33277 Potri.012G127500.1.v4.1 977 777.512 24482 569.435 ==> SRR26075332.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 656 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 777 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 2 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 27 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 995 SRR26075332 completed mapping pipeline successfully