Starting /dee2/code/volunteer_pipeline.sh SRR26075333 current disk space = 3053338804224 free memory = 1426668248 SRR26075333 SRAfilesize 93880c1e90062c29ae74ff7683c97866 SRR26075333.sra SRR26075333.sra file validated SRR26075333 is paired end SRR26075333 is conventional basespace SRR26075333 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075333_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.54675 37.0 37.0 37.0 37.0 37.0 2 36.6145 37.0 37.0 37.0 37.0 37.0 3 36.644 37.0 37.0 37.0 37.0 37.0 4 36.665 37.0 37.0 37.0 37.0 37.0 5 36.711 37.0 37.0 37.0 37.0 37.0 6 36.681 37.0 37.0 37.0 37.0 37.0 7 36.598 37.0 37.0 37.0 37.0 37.0 8 36.6815 37.0 37.0 37.0 37.0 37.0 9 36.7065 37.0 37.0 37.0 37.0 37.0 10-14 36.6522 37.0 37.0 37.0 37.0 37.0 15-19 36.6016 37.0 37.0 37.0 37.0 37.0 20-24 36.564 37.0 37.0 37.0 37.0 37.0 25-29 36.481100000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.4403 37.0 37.0 37.0 37.0 37.0 35-39 36.3794 37.0 37.0 37.0 37.0 37.0 40-44 36.36449999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.1937 37.0 37.0 37.0 37.0 37.0 50-54 36.2039 37.0 37.0 37.0 37.0 37.0 55-59 36.1179 37.0 37.0 37.0 37.0 37.0 60-64 36.0775 37.0 37.0 37.0 37.0 37.0 65-69 36.0127 37.0 37.0 37.0 37.0 37.0 70-74 36.0177 37.0 37.0 37.0 37.0 37.0 75-79 36.0758 37.0 37.0 37.0 37.0 37.0 80-84 36.04970000000001 37.0 37.0 37.0 37.0 37.0 85-89 35.9373 37.0 37.0 37.0 37.0 37.0 90-94 35.97259999999999 37.0 37.0 37.0 37.0 37.0 95-99 35.882600000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.9114 37.0 37.0 37.0 37.0 37.0 105-109 35.8111 37.0 37.0 37.0 37.0 37.0 110-114 35.5728 37.0 37.0 37.0 37.0 37.0 115-119 35.5795 37.0 37.0 37.0 37.0 37.0 120-124 35.6203 37.0 37.0 37.0 37.0 37.0 125-129 35.452600000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.4357 37.0 37.0 37.0 34.6 37.0 135-139 35.315 37.0 37.0 37.0 32.2 37.0 140-144 35.284499999999994 37.0 37.0 37.0 29.8 37.0 145-149 35.2734 37.0 37.0 37.0 34.6 37.0 150-151 35.2195 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 1.0 21 2.0 22 2.0 23 5.0 24 5.0 25 6.0 26 13.0 27 15.0 28 20.0 29 24.0 30 33.0 31 40.0 32 39.0 33 110.0 34 155.0 35 413.0 36 2901.0 37 215.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 46.897764380808844 14.594323034413465 6.7319768902285855 31.775935694549105 2 20.150000000000002 14.875 32.324999999999996 32.65 3 17.65 20.05 30.075000000000003 32.225 4 21.975 24.85 24.875 28.299999999999997 5 23.599999999999998 31.3 24.75 20.349999999999998 6 23.125 32.675 23.875 20.325 7 14.299999999999999 27.775 40.575 17.349999999999998 8 16.875 27.175 30.625000000000004 25.324999999999996 9 19.55 24.325 32.800000000000004 23.325000000000003 10-14 20.135 29.725 26.96 23.18 15-19 19.869999999999997 27.845 28.765 23.52 20-24 19.650000000000002 29.235 27.48 23.635 25-29 20.064999999999998 28.485 27.625 23.825 30-34 19.7 27.435 28.515 24.349999999999998 35-39 19.615 28.13 28.43 23.825 40-44 20.005 28.99 28.310000000000002 22.695 45-49 20.745 28.095 27.875 23.285 50-54 20.06 28.985 26.915 24.04 55-59 20.535 28.37 27.255000000000003 23.84 60-64 20.369999999999997 27.935 27.66 24.035 65-69 20.5 28.035 27.245 24.22 70-74 20.845 28.585 26.21 24.36 75-79 20.535 28.000000000000004 27.58 23.885 80-84 20.845 27.92 26.705000000000002 24.529999999999998 85-89 21.05 27.825 26.765 24.36 90-94 21.634999999999998 27.794999999999998 26.43 24.14 95-99 21.385 27.165 28.1 23.35 100-104 21.15 27.83 27.655 23.365 105-109 21.66 27.155 26.96 24.224999999999998 110-114 21.349999999999998 27.189999999999998 28.235 23.225 115-119 21.18 26.965 28.34 23.515 120-124 21.455 26.895000000000003 27.034999999999997 24.615000000000002 125-129 21.145 28.425 27.105 23.325000000000003 130-134 22.625 27.310000000000002 27.165 22.900000000000002 135-139 22.155 27.584999999999997 26.740000000000002 23.52 140-144 21.91 27.839999999999996 27.13 23.119999999999997 145-149 21.695 27.42 26.755000000000003 24.13 150-151 21.975 27.712500000000002 25.974999999999998 24.337500000000002 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.5 5 1.0 6 1.0 7 0.5 8 0.0 9 1.0 10 1.0 11 1.0 12 1.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.5 18 1.0 19 0.5 20 0.5 21 0.5 22 1.0 23 1.0 24 3.0 25 7.5 26 6.5 27 9.5 28 16.5 29 22.0 30 24.5 31 35.5 32 34.0 33 28.5 34 40.0 35 55.0 36 78.0 37 92.5 38 126.5 39 145.5 40 166.0 41 196.0 42 219.5 43 247.0 44 273.0 45 274.0 46 248.5 47 237.0 48 232.0 49 198.0 50 166.5 51 168.0 52 137.0 53 102.0 54 89.0 55 74.5 56 52.0 57 34.5 58 26.5 59 24.0 60 17.5 61 12.5 62 10.0 63 5.0 64 4.0 65 5.0 66 9.5 67 11.0 68 8.0 69 5.0 70 3.0 71 1.5 72 1.0 73 0.5 74 0.5 75 0.5 76 0.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.475 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.675000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 63.74124433456942 38.675 2 20.601565718994642 25.0 3 8.817470127729706 16.05 4 3.419859909353111 8.3 5 2.142562834775443 6.5 6 0.6592501030078286 2.4 7 0.2472187886279357 1.05 8 0.288421920065925 1.4000000000000001 9 0.04120313143798929 0.22499999999999998 >10 0.04120313143798929 0.4 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGCTGTTATCTCGTAT 16 0.4 TruSeq Adapter, Index 10 (97% over 38bp) CTCCCACTGACCTATGCCCTTTGAGCTGAATCATCTTCTCCTTAGCAGCT 9 0.22499999999999998 No Hit GGCAGAATAATACCTGGACGACATCAATACCAGCAGCTAGGTAAACCTCA 8 0.2 No Hit CCTGGAAGAAAGAGGTCTGGGATGGGTCAAGCCCTGTGTTGCCAGGAGGG 8 0.2 No Hit CAGCTACTGGCTTTTCTGCCCCAGGTCCCATGGCTAATCGTTCTTCCCTC 8 0.2 No Hit CGGGAGTTTTTTGCAGCACCTTTTGGGGATTCAATGTTCTCCAGCGTTGC 8 0.2 No Hit AGAAAAAGGACTCCAATGGCCAGTAGCCGCAGCAGCTACTTAATTAGTAC 8 0.2 No Hit CAGACCCTCCACTACTCTTCCAAACACCACGTGTTTTCCATCGAGCCATT 8 0.2 No Hit TCATTCAGAGCATAAAAGTCACAAGCAAAACTCCATATCGTTAAGTTTCT 8 0.2 No Hit GCCCAGTAGCACGATATTTCGCTGCCATCTCCTCTGCTTTTAAGAATTCT 7 0.17500000000000002 No Hit CCTGGATTTGGTCTCCACAGGAATAGGATCTTGCTGGCAGGTTTGGCCAT 7 0.17500000000000002 No Hit GTACTTTCTTATTCATCGTGTGATTCTTTTGCAGTAGTTTCCTACCAAAA 7 0.17500000000000002 No Hit GTGCAAGCGGTATCACTGCAACGCTAGCCGATATAATAATCACCGCTGGA 7 0.17500000000000002 No Hit TGGAGATTGGAATATTTTGATGAGAAATCTGGCAAAAATCCACTCATCTT 7 0.17500000000000002 No Hit GGCCTGATGTTGGGATTGCAAACCAAAAAGTGTGTTATTTGAAAGCACAT 7 0.17500000000000002 No Hit CCCTTCTACATGTATCCCTGTCATATGCATCCTTGCCCACATTCCAAATC 6 0.15 No Hit GTGGTGATGGGAAGCCAGAAAACTTCCTTGGGTGCTTCACTTGGAGAGAA 6 0.15 No Hit GGGCCCCACTTGTTGACACCATTAGGCCTGGCATTGAGAAGGATCTGATT 6 0.15 No Hit ATTTCTTCTTCTTCTTTTGCTCAGCTGGACCCAGCAACCAGCTCTGCTGA 6 0.15 No Hit GCCTCATGAGTTGTGAAAGCGAATTGACCATGAGTCACATTCTCCTTATG 6 0.15 No Hit GGAGGATAGCCTTGAGGGGGATATGCTCCAGGCTGTGGAGGATATCCAGG 6 0.15 No Hit TGATATTTTTGAATGGAGGGTAGATAAGTCCCTTATCAAAGTTCTCCTGG 6 0.15 No Hit AGGAAGTCTCCGTATGCTTTATTTCGAACGTAAAAATCAGAAGTAGAACT 6 0.15 No Hit GTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTA 6 0.15 No Hit CTCACGTAAATAGTTCCCATATCAAAATAACATTTTACATATTGATAGAG 6 0.15 No Hit GCAGCGGAGTGTAATTTATGGTCCAATAAAGCCCCATTTATTCGTGAAGC 6 0.15 No Hit GAGCTCCTGACTCGAGTTCTAGCAACCTCTCCTCGTCTACGTTTAGCAGA 6 0.15 No Hit CTTCAGTGATGGCTCGATTTCACTTACATCCTTGATCTTGAAGAGATCGG 6 0.15 No Hit CCTCCTCGAAGAAGATTAAGCAGTTGCAATTGGACCCAGATTGACAGTGG 6 0.15 No Hit GAAGGCTTGCATGACATTAGGCCAGCAACAGGCATTTTGCATATGGTACT 6 0.15 No Hit CCCTTGAGCTCCTCTTCAAGTGCTTCTTCAAGCCAAAATCTTGATTCTTG 6 0.15 No Hit CCTGAAAGAAGTTCTGCCAACACAAGAAATTAAGACGGGCATTTTATTTA 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGCTGTTATCGCGTAT 5 0.125 TruSeq Adapter, Index 10 (97% over 38bp) CAGCATTTATCTCATCAAGGGCACGCCATTGGATCTTGGGAAATCCAGGG 5 0.125 No Hit GTCCGGCCGAAGAGGACCAGAGGCTGATCGGAAGCGCGGAAGAGATACAA 5 0.125 No Hit TTTTTACCCCCATTCATTTGCATGTTGTTACCATTACCACCATTGTTCTT 5 0.125 No Hit ACCGTATCCAAAATACTGGTGCGATTCCATACCTACACGTTTAGCATCAG 5 0.125 No Hit GTCGGTCTTGCAATAGCCAGGGGAGACACAATTGGCATGGAAATTCGGGT 5 0.125 No Hit CTCCGTGAATGCTGAATCTAACTAGAAGGATTTGCAAAGTTGTTGCCATA 5 0.125 No Hit AGTGACTCCGGTGATTTTGCTTTGCCTGCCGCTGACACGTGTCAACGATC 5 0.125 No Hit GCTTATAACATAGAAGCATGCGCATGGATGGGGTTTAGATAAATGATGGA 5 0.125 No Hit CAAGAAAGTGATCAGAATTGAAAATTCAAGAAAGGGACAACAAAATAAAC 5 0.125 No Hit GACATGGGCAGGTAGAGTGACATCCTTGTGGTCAGTTTTCAACTTCAGAT 5 0.125 No Hit CATTGCAGGATAAAGTCCCGCGCGTCATTTGAGAGAGAATCAGGAACAAG 5 0.125 No Hit ACGGACTTGTGCCAGAGGTGATATGCGAGGACTAATGCCTCCTTCACGCT 5 0.125 No Hit GTCATCAATAACACCGTTGCCATTGGCGTCTGCTGCCTTTACCCCCCTCT 5 0.125 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.125 No Hit GGGGGGGTAGAACCACGGCGACGGATGGCCGGTAGATATAGGGCCATGTC 5 0.125 No Hit GAGATTTTACCATAAAGAAGAGCCCCATCGTGGTATCGCAAGAGTTGAGG 5 0.125 No Hit GTTGATGATGAGTTTTCTGATCTCTATGTATTCAAATGGACATCTCTTAT 5 0.125 No Hit GCCGGTTGTAGGGAAATTAGTTTTGGCTTTGGCTCCGCGGAATTCACGAG 5 0.125 No Hit CTTGTCTGTGGGTCTCTTGGGCTCACTGATCTGGTCAAGAGCATCAAGGA 5 0.125 No Hit CAGCAGATCCGGATCCGAGGATGAGGACACCTTCCTCTCTGAGAGGGGCC 5 0.125 No Hit TAAGTATCTCCTGTTCCTTTAGTCGATGCTGGAACCTAGCAAGGCGTGCT 5 0.125 No Hit GTGACAAGCTCGGTTAAGCAGCATACAGGCAGGTCCAAGGATGTCATCAA 5 0.125 No Hit AACACTTCCACTCTGATCACAATCAAACTTGTCAAAGATTGAGTCATACA 5 0.125 No Hit GCCGAAGTCAACCTCTCTTTTCTTTTCTCCAAAAACCTCTGCAGTGATTT 5 0.125 No Hit TTCTGGCGGAGGTCGGTGGAGAGAGGAGCGCTCATTAGGATCCTGCGCAA 5 0.125 No Hit GGCATTGCTTGTACCAGGATCAACACCACTGCCAGCAGGTACACCTGATG 5 0.125 No Hit GGGGACTTTAACAAGAAACAAACAGAAAAATCTCCGAATCTAAGAAACAG 5 0.125 No Hit CCCCACTTGCTTTCTTGAAACAGCTACTGTTGCTGGCATTAAGCTTCTTG 5 0.125 No Hit GACAGAATTCGATAGCCACCAGTGAGTTCTGTTGAAGGCATGTAAGTACC 5 0.125 No Hit GTAAAGATTGACACGAGTCGGGGACGCTGATCGTTGATGAGAATTCTGAA 5 0.125 No Hit GGAACATTCAAAAAAAAGAATAGCATCAATGTACCACTCGAGGTCTCTTT 5 0.125 No Hit GCACCGCTCACCGTTGAAAGCAATGACACTAATCCAATAGCCACATGATA 5 0.125 No Hit CCAAGACTGCAACATTATCATCGAAAGAAGCTTTTGAAAACTCTTCAAAA 5 0.125 No Hit ATTTTCTCTTCACTTCTCTAAAACTTCTTTTTTTCCTTGAGGAGGGAGGG 5 0.125 No Hit TGTTGGTTAAGTCTTTGGAGCTCCTCTTCTGCTTGCTTTAACTCCTTCTC 5 0.125 No Hit ATGGGCTCTTCCTCAACTGCAGTCCAAGCGGACTAGGCTCATCTAACGGA 5 0.125 No Hit AGACGAACTAGGTTTACATGCCAACATGGTTATAACATCACAAGCCAACT 5 0.125 No Hit GCTACATCTGTCTATGTTTGATCAAGAAGATCATTATTTGGGTAATTACA 5 0.125 No Hit GCCACATTCATTGCAATGGTACTGCTGCTTTGAAATATCGTCATCGAAGA 5 0.125 No Hit AGTGCATATCCAGTTACACAGTAACTAATTCCTAAATTAAAAGAAAAAAA 5 0.125 No Hit CTTTACCTCATTGGTTGCTTGATCAGCGGAATAAAGTTGATTATTTACAA 5 0.125 No Hit GAGTTGGGCCCTCTCTTCTTGCCAAATCCCACAACTGCTGTGACGAAACG 5 0.125 No Hit CTTCTTCTTATTTTTCTCTGATTTCTCCTTGTCCTTCTCTTTATGCTGCT 5 0.125 No Hit GTGAGTTTATGACATGGTTAGTCTTCTCCATGAGGGTGGCATCAAAGGAT 5 0.125 No Hit GGCCCGTTCAAACGAATCTTCAAGTCATCACACTCAACCTTGGGCTTGAA 5 0.125 No Hit GCCCACTCCGCAGCAACAGGCTTCAATTCATCCACGTCCACCTTCAAGAA 5 0.125 No Hit CGTTTTTAACTTTGCAAGGGAAAGTTGCTTGGATCCGATGCTTTCGCTTC 5 0.125 No Hit AATGGATCGAGGCTCGGCCAAGGTGGACCTGAACAACAAACTAACAGTAG 5 0.125 No Hit GCAACCTTGTTTTTCATTTTCTCTGCGTATTCTGCCTTTTGTTTCTCCAA 5 0.125 No Hit GGTAGTGCTAAAGGAACATTAAAATAAAAATCTGGATATCACATATATTT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.1 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.16249999999999998 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.175 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.175 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.175 0.0 0.0 0.0 0.0 88-89 0.175 0.0 0.0 0.0 0.0 90-91 0.275 0.0 0.0 0.0 0.0 92-93 0.3 0.0 0.0 0.0 0.0 94-95 0.3 0.0 0.0 0.0 0.0 96-97 0.4 0.0 0.0 0.0 0.0 98-99 0.45 0.0 0.0 0.0 0.0 100-101 0.45 0.0 0.0 0.0 0.0 102-103 0.45 0.0 0.0 0.0 0.0 104-105 0.5249999999999999 0.0 0.0 0.0 0.0 106-107 0.6375 0.0 0.0 0.0 0.0 108-109 0.7625 0.0 0.0 0.0 0.0 110-111 0.8625 0.0 0.0 0.0 0.0 112-113 0.9624999999999999 0.0 0.0 0.0 0.0 114-115 1.1124999999999998 0.0 0.0 0.0 0.0 116-117 1.225 0.0 0.0 0.0 0.0 118-119 1.5 0.0 0.0 0.0 0.0 120-121 1.7 0.0 0.0 0.0 0.0 122-123 2.0 0.0 0.0 0.0 0.0 124-125 2.3 0.0 0.0 0.0 0.0 126-127 2.75 0.0 0.0 0.0 0.0 128-129 3.25 0.0 0.0 0.0 0.0 130-131 3.9 0.0 0.0 0.0 0.0 132-133 4.4625 0.0 0.0 0.0 0.0 134-135 4.825 0.0 0.0 0.0 0.0 136-137 5.8625 0.0 0.0 0.0 0.0 138-139 6.3625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGGATC 10 0.006830828 145.0 9 TCAATGG 10 0.006830828 145.0 145 ACATACA 10 0.006830828 145.0 4 AGATCCG 10 0.006830828 145.0 5 CATACAA 10 0.006830828 145.0 5 ACAACTT 10 0.006830828 145.0 8 AACATAC 10 0.006830828 145.0 3 >>END_MODULE SRR26075333 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075333_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.6485 37.0 37.0 37.0 37.0 37.0 2 36.108 37.0 37.0 37.0 37.0 37.0 3 36.04 37.0 37.0 37.0 37.0 37.0 4 36.1555 37.0 37.0 37.0 37.0 37.0 5 36.12 37.0 37.0 37.0 37.0 37.0 6 36.05 37.0 37.0 37.0 37.0 37.0 7 36.1085 37.0 37.0 37.0 37.0 37.0 8 36.054 37.0 37.0 37.0 37.0 37.0 9 36.11 37.0 37.0 37.0 37.0 37.0 10-14 36.03340000000001 37.0 37.0 37.0 37.0 37.0 15-19 35.912400000000005 37.0 37.0 37.0 37.0 37.0 20-24 35.8658 37.0 37.0 37.0 37.0 37.0 25-29 35.6656 37.0 37.0 37.0 37.0 37.0 30-34 35.506800000000005 37.0 37.0 37.0 37.0 37.0 35-39 35.4902 37.0 37.0 37.0 37.0 37.0 40-44 35.418699999999994 37.0 37.0 37.0 37.0 37.0 45-49 35.402 37.0 37.0 37.0 37.0 37.0 50-54 35.131499999999996 37.0 37.0 37.0 37.0 37.0 55-59 35.2236 37.0 37.0 37.0 37.0 37.0 60-64 35.310900000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.19539999999999 37.0 37.0 37.0 37.0 37.0 70-74 35.0823 37.0 37.0 37.0 32.2 37.0 75-79 35.00339999999999 37.0 37.0 37.0 27.4 37.0 80-84 35.0616 37.0 37.0 37.0 29.8 37.0 85-89 34.9779 37.0 37.0 37.0 27.4 37.0 90-94 34.9533 37.0 37.0 37.0 25.0 37.0 95-99 35.005900000000004 37.0 37.0 37.0 29.8 37.0 100-104 34.9015 37.0 37.0 37.0 25.0 37.0 105-109 34.9311 37.0 37.0 37.0 25.0 37.0 110-114 34.842200000000005 37.0 37.0 37.0 25.0 37.0 115-119 34.8788 37.0 37.0 37.0 25.0 37.0 120-124 34.724399999999996 37.0 37.0 37.0 25.0 37.0 125-129 34.8283 37.0 37.0 37.0 25.0 37.0 130-134 34.7665 37.0 37.0 37.0 25.0 37.0 135-139 34.5346 37.0 37.0 37.0 25.0 37.0 140-144 34.5983 37.0 37.0 37.0 25.0 37.0 145-149 34.5427 37.0 37.0 37.0 25.0 37.0 150-151 34.3565 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 4.0 14 13.0 15 16.0 16 17.0 17 18.0 18 6.0 19 13.0 20 2.0 21 14.0 22 14.0 23 14.0 24 15.0 25 14.0 26 25.0 27 16.0 28 12.0 29 25.0 30 25.0 31 30.0 32 62.0 33 103.0 34 255.0 35 780.0 36 2352.0 37 155.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.0 23.674999999999997 7.675 18.65 2 29.825000000000003 25.674999999999997 25.874999999999996 18.625 3 23.200000000000003 30.0 30.275000000000002 16.525000000000002 4 26.125 33.650000000000006 23.674999999999997 16.55 5 26.474999999999998 36.75 18.85 17.925 6 26.275 35.3 20.525 17.9 7 22.525000000000002 24.0 35.775 17.7 8 24.224999999999998 25.174999999999997 26.724999999999998 23.875 9 25.474999999999998 23.775 27.950000000000003 22.8 10-14 25.955000000000002 28.95 24.93 20.165 15-19 26.540000000000003 28.78 25.0 19.68 20-24 25.645 28.775000000000002 25.895000000000003 19.685 25-29 25.319999999999997 29.020000000000003 25.545 20.115 30-34 25.240000000000002 27.860000000000003 26.46 20.44 35-39 25.169999999999998 28.544999999999998 25.885 20.4 40-44 25.745 28.749999999999996 25.85 19.655 45-49 25.34 28.415000000000003 26.540000000000003 19.705000000000002 50-54 24.310000000000002 28.625 26.8 20.265 55-59 24.73 28.994999999999997 26.424999999999997 19.85 60-64 25.31 28.494999999999997 25.85 20.345 65-69 25.365 28.345 26.345000000000002 19.945 70-74 24.685000000000002 29.575000000000003 26.255 19.485 75-79 24.58 29.475 26.640000000000004 19.305 80-84 25.495 28.050000000000004 26.205000000000002 20.25 85-89 25.785000000000004 28.294999999999998 27.3 18.62 90-94 25.419999999999998 28.134999999999998 26.615 19.830000000000002 95-99 24.5 28.985 26.115 20.4 100-104 25.445 29.304999999999996 26.07 19.18 105-109 25.545 27.589999999999996 27.33 19.535 110-114 25.555 28.000000000000004 26.88 19.564999999999998 115-119 25.235000000000003 28.299999999999997 27.11 19.355 120-124 24.785 28.410000000000004 27.32 19.485 125-129 25.355 27.68 27.075 19.89 130-134 26.005 28.265 26.085 19.645000000000003 135-139 24.745 28.54 26.740000000000002 19.975 140-144 25.64 28.105000000000004 26.19 20.064999999999998 145-149 26.529999999999998 28.275 25.865 19.33 150-151 26.825 27.3125 27.6375 18.224999999999998 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 1.5 6 1.5 7 1.0 8 1.5 9 0.5 10 2.5 11 3.0 12 1.0 13 1.5 14 1.5 15 2.0 16 1.5 17 0.0 18 1.0 19 1.0 20 0.0 21 1.0 22 2.0 23 2.5 24 2.0 25 2.5 26 4.5 27 10.5 28 9.5 29 7.0 30 10.0 31 11.0 32 11.0 33 19.5 34 32.0 35 43.0 36 64.0 37 98.5 38 118.0 39 152.5 40 195.0 41 222.0 42 258.5 43 277.0 44 290.5 45 286.5 46 251.0 47 231.5 48 225.5 49 207.0 50 191.0 51 160.5 52 120.0 53 94.5 54 73.0 55 51.5 56 35.0 57 24.5 58 16.0 59 11.0 60 11.0 61 9.5 62 9.5 63 11.5 64 7.5 65 4.0 66 4.0 67 2.5 68 2.0 69 1.5 70 1.5 71 3.0 72 2.5 73 2.0 74 1.5 75 0.0 76 2.0 77 3.0 78 2.5 79 3.5 80 4.0 81 4.5 82 5.0 83 3.0 84 2.5 85 2.5 86 2.5 87 3.0 88 1.5 89 2.0 90 3.5 91 3.5 92 3.0 93 3.0 94 2.5 95 2.0 96 2.0 97 2.5 98 2.0 99 1.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.5 #Duplication Level Percentage of deduplicated Percentage of total 1 65.85365853658537 40.5 2 19.0650406504065 23.45 3 8.86178861788618 16.35 4 3.048780487804878 7.5 5 1.8292682926829267 5.625 6 0.6097560975609756 2.25 7 0.3252032520325203 1.4000000000000001 8 0.24390243902439024 1.2 9 0.08130081300813008 0.44999999999999996 >10 0.08130081300813008 1.275 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 33 0.8250000000000001 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 18 0.44999999999999996 No Hit GAAAGCATCCACAAATCAAAGGCAGAGAAGGCTAGGGAGAAGACTTTGTC 9 0.22499999999999998 No Hit GCAAGGGGTTCTATAGGTGTCCTGTTGACAAATCTGTGAGGTCATTGATG 9 0.22499999999999998 No Hit GCCACTAAAGCGCTCAAGGATATGAAGTTAAGAAAATGCTATTCAGATAA 8 0.2 No Hit GTTAGGATCCATTCCGAAAAAACTGGAAACAAAGCTTTCCTCAACCTTAT 8 0.2 No Hit GATACTGAGGCCAGAAGGAAATCTGATCGTGCGTGACAATGTTGAAATCA 8 0.2 No Hit GCAGAAAGGGTGGCAGAAGGGGAAGAAGGCTGTAAGCTGTCTCTCATCAG 8 0.2 No Hit AAGGAAAAGGCCGAAGCGGCAAGCCTTTACACTACAAAGGCTCGACTTTC 8 0.2 No Hit ATATCTTGGTGACAAGAGGAAATTATTCCGAATTATGGACACCAAGTTGG 8 0.2 No Hit GTTCTCTGCCTTTTTGTTTTTGTTTTTGTTTTCGTTTTCTTGAATTGAGG 7 0.17500000000000002 No Hit CTGGATATCTCGAACACTGGAATTTCAGATACAATCCCTGATTGGTTTTG 7 0.17500000000000002 No Hit GCCACAAGGCCAGTGCTGTCATGTCATCATTGATGAGCATAGCTCCACAG 7 0.17500000000000002 No Hit GAAGAAATTCGGATCGGAGAAAGGTAAACTGGTATTTGGAGATTTCAATT 7 0.17500000000000002 No Hit GTTCAGACCTGGATTAGTGCTGGATTGACTGATGAAAACACTTGTGTTGA 7 0.17500000000000002 No Hit GATTGCATTGTTGTGGAGTGACTTGTATTTCTGTTAGATATTTGCATGAT 7 0.17500000000000002 No Hit GCTGCAAAGCCCAGGAAGTTTTGGTTTCTCAGGATTTGGAAACCTCATGG 7 0.17500000000000002 No Hit GGCATGGGAAGACAGCGAGAAAACTAAAGCGGAAAACAAGTCTCAGAAAA 7 0.17500000000000002 No Hit GCAGCTTTCTTGGGTTTGCAAGATTTGAGTGAGGATGAGCTTGTTAGTAT 6 0.15 No Hit GTATTCCAAGAAATGGAGAAAGTCCAAAAGCTCTTTTGTGTGGCTCTATT 6 0.15 No Hit TGATCTGTAGCTGCAATTTTTTCATGGTTAGCGAAGCCGTATGGTTGAAT 6 0.15 No Hit CATCATGCTATTATGAATTTCACATGTCTTGAGATGAGGGACTCAGAACA 6 0.15 No Hit GGGTGACAATGTAGGAGACATTGCAGGGATGGGGTCTGACCTGTTTGGAT 6 0.15 No Hit AACGGATGGCACTAGGATGTCAGTAGCTTCTTTCTATAACCCTGGAAGTG 6 0.15 No Hit AGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCC 6 0.15 No Hit GTAAGGATGCTGAAGCTGCTCAACAAGATTCAAATCAGAATATCGAAACA 6 0.15 No Hit CAGAAATGAGGGTTCTTCGCCAATCTACTGGTGATGACCACTTGGTATTG 6 0.15 No Hit GGAGAGCTATACAACTATCTTTATCAATATGTAAAATGATCAGAACTATT 6 0.15 No Hit AAGTGATTGAAGCCATGGCATCCTTCAATGAGAAACCTATAATTCTCTCT 6 0.15 No Hit ACTATGTTCCTGCAACAGAAGTTATCGACAGCAACGGGGCAGCTCAACGC 6 0.15 No Hit CCTAACCAACAAACAAACCCCCCGAAAAATAAAAATAGGAAACCCCCAAC 6 0.15 No Hit ATTATAGCAGCTCAGAAACCGAGGAAGAAGGTCCAGCTCACTCTGATGGA 6 0.15 No Hit GTGTGCTAGAGCTTGCCAAGAGTTTCCTTCAAACATGAGTCAGAATCAGG 6 0.15 No Hit GGCTTGGTCTCAATCTCATCAAGATGCTTCATCAGTTCTGCAGTGCAAAA 5 0.125 No Hit GTTTGGTTATAGTTCCTTGACTCCGTTCTATCAAGGACACAAGAACACTA 5 0.125 No Hit GGGGATTTTACCTTGATCGGGCTTGAGATTCTTAGAGATGTAAGAGAAGC 5 0.125 No Hit GAGAAATTTATGCAAAGAAGCTCACTAACTTTGTCGAGAAACGACTGAAA 5 0.125 No Hit AAATCTTGCAAGGGGACTGCATTCTGGATGGCTCCTGAGGTTGTCAATAA 5 0.125 No Hit AACAACGAAAGGGCTCTGCAAGACTCTGGACTCTATAGCCCTGACAGTGA 5 0.125 No Hit ATCCAAAGTTCTGAAATACGGATCAACGGTGTTTAGAGCCAACATGGGAC 5 0.125 No Hit CACCATCGATGCTTAACCCTAACCTTCACTATATACTTAATTTCTCGAGA 5 0.125 No Hit GGAATGGTGGATTACAGGGAGTTTAAGAAGATGATGAAGGGTGGTGGTTT 5 0.125 No Hit AGAAAGGTAAACTGGTATTTGGAGATTTCAATTATCCAGAGAATGGAGAG 5 0.125 No Hit CGATTGCAGGCATTGTCATAATGAATCAAAGAATTCTATGGAAGTTAATC 5 0.125 No Hit CTTGGATGTATTAGTTGACCGTCTCGCAGGTCGGCAACAACTTGGTGAAG 5 0.125 No Hit GGTGTTTTTTCTGCATATGGCACAAGCAGCGTTTTGTTTCAATGGTTTCG 5 0.125 No Hit TCCATCTGTTTCTTTCTAAAAGCAAGATTCGTCTGTATCAGTTGAAGAAG 5 0.125 No Hit CAATATTTCTGTTTTCAGATGTTAAAGTTTTACTTTAAAGCTTGTTGCTT 5 0.125 No Hit CTTTGGAATGTATGATTCTCTTAAACCTGTGGTTTTGGTTGGTAATTTGC 5 0.125 No Hit CGACGAATTCAGACTGAAAGTGACGAAGAATTCAGACTGAAAGTCCAGTG 5 0.125 No Hit CTCTTCCCTGCAGCGCCCGCCTCCCAAATACCCTTAACCCACATAGCCAG 5 0.125 No Hit GGGAAAGATGTGCTTGGTATTTGTGTGTGATGAGGATGAGAAGGTGGTAG 5 0.125 No Hit CATGTTGATGAAGATAAGAAACGAGGGCTCGACCACGGTGCTTGGGTTCC 5 0.125 No Hit ATGGAGAAAGCAAAGTTCTCAGACAATGAAGTAACCATGATAAGGGGTTT 5 0.125 No Hit GTGAACTTCATTTAATCAAATTTCAGGTCCGCCCTAGAGAGAGAAACAAG 5 0.125 No Hit CTATCAAGGAATCCTTAGAGTCTGCGCATGCTGCTCATATGGAAGCAGAG 5 0.125 No Hit TGGTGCTGTTGGGGATTTGTACATGCACATTTGTGAAGATGCACTTTCCA 5 0.125 No Hit GAACTGCAGGCTAGAGCAATCCTAATGCTTGCAAGTCGAGAAATGGAGGA 5 0.125 No Hit GTACTACCCACCCACCTCTTCCTCCCTGTTTCAAAACTCAAACACATATC 5 0.125 No Hit GTAAAAATGGAAATGGAGGGAAAAAAGGTGGTGGTGCTGGTGGGGGAGGA 5 0.125 No Hit CATTTCTTGCGTTACTGTTATTTATTAGGTTTCCATGTAGCTTAGATAAC 5 0.125 No Hit GAAATAGAGGATACAATGGAGATTGTTCGTGAAGAAATGAAACTGTTGGC 5 0.125 No Hit CACGAGTATATCTTAGCTCACTCTTCTAATCCTTATCTAGTAGTCTCCTC 5 0.125 No Hit ACCAAACCTGAAAACCCATCAAAGAAAACTACAAAGAAAACAAAGTCAAA 5 0.125 No Hit GAGAGGCCAAACTCCCAAAGTGGCAAAGCAGGACAAGAAAAAGAAGCCCC 5 0.125 No Hit GTTCAAAGCAGCACTCATTCCCAATTCGCGGAAGATTTTAGCTCCTACGA 5 0.125 No Hit GATGCAAAAAGGATTGGAGGATATGGAATAAGCTGGTTTCTGAAACCGGT 5 0.125 No Hit GGGTGTCTTTCCACGTCTTCCCATAAGCCCTTTTGCAAAGGGGTAGAGAT 5 0.125 No Hit GCATTTTACTATTTACCATGTTCCCTGTGTTTATATTGCTTGCAGCTTGC 5 0.125 No Hit AAAACAGCTTGCAGAAAAGGAGATCAAGCTGGGTGCCAAAATCTGGTTGC 5 0.125 No Hit GTGAAGGCTAAAATCCAAGATAAGGAGGGCATCCCTCCAGACCAACAGAG 5 0.125 No Hit GCAAGAGCAGTACTAGTGTGAGCTTCCAGATTAATGGGAAGGAGTTTGAG 5 0.125 No Hit AACTAATCGACCGATCCGCTTTCTCCAAACCCGAATCCATATCCGAAGCC 5 0.125 No Hit GCTGTTGTTGAAAAGACAGAACCTGCTCCGAAAAAGATTTCAGGTGGATC 5 0.125 No Hit AATCAGTAGCCGATCCAAGAACAGATCACCAACAAAAGCTTCTTGAGAGT 5 0.125 No Hit GAACGACGTCGAGTTCGTCGTCTTCGCTGGATAAAAGGGGAGCAGTGAAG 5 0.125 No Hit GCTAGTTAGTACACCCATCATATTTCCTGATTGATATACTCCCTTTTGTC 5 0.125 No Hit GCAAGCTTTTATTCTTCAACAATGGCGGCCCCTTCTTCACAGTCGAGAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.075 0.0 0.0 0.0 0.0 64-65 0.0875 0.0 0.0 0.0 0.0 66-67 0.1 0.0 0.0 0.0 0.0 68-69 0.1125 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.125 0.0 0.0 0.0 0.0 84-85 0.125 0.0 0.0 0.0 0.0 86-87 0.125 0.0 0.0 0.0 0.0 88-89 0.125 0.0 0.0 0.0 0.0 90-91 0.225 0.0 0.0 0.0 0.0 92-93 0.25 0.0 0.0 0.0 0.0 94-95 0.25 0.0 0.0 0.0 0.0 96-97 0.35 0.0 0.0 0.0 0.0 98-99 0.4 0.0 0.0 0.0 0.0 100-101 0.4 0.0 0.0 0.0 0.0 102-103 0.4 0.0 0.0 0.0 0.0 104-105 0.475 0.0 0.0 0.0 0.0 106-107 0.5874999999999999 0.0 0.0 0.0 0.0 108-109 0.7124999999999999 0.0 0.0 0.0 0.0 110-111 0.8375 0.0 0.0 0.0 0.0 112-113 0.9375 0.0 0.0 0.0 0.0 114-115 1.0875 0.0 0.0 0.0 0.0 116-117 1.2000000000000002 0.0 0.0 0.0 0.0 118-119 1.475 0.0 0.0 0.0 0.0 120-121 1.675 0.0 0.0 0.0 0.0 122-123 1.9749999999999999 0.0 0.0 0.0 0.0 124-125 2.3125 0.0 0.0 0.0 0.0 126-127 2.775 0.0 0.0 0.0 0.0 128-129 3.2750000000000004 0.0 0.0 0.0 0.0 130-131 3.875 0.0 0.0 0.0 0.0 132-133 4.4375 0.0 0.0 0.0 0.0 134-135 4.8 0.0 0.0 0.0 0.0 136-137 5.824999999999999 0.0 0.0 0.0 0.0 138-139 6.3125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTTGA 10 0.006830828 145.0 7 GGTTGAT 10 0.006830828 145.0 8 GAAATAA 10 0.006830828 145.0 5 CTGGTTG 10 0.006830828 145.0 6 CTAGCTG 10 0.006830828 145.0 2 CCTAGCT 10 0.006830828 145.0 1 ATAAAAA 45 0.008957279 48.333332 8 >>END_MODULE Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345826 spots for SRR26075333.sra Written 3345826 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra Read 3345822 spots for SRR26075333.sra Written 3345822 spots for SRR26075333.sra SRR ids: ['SRR26075333.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_gvzjz11d SRR26075333.sra spots: 66916444 blocks: [[1, 3345822], [3345823, 6691644], [6691645, 10037466], [10037467, 13383288], [13383289, 16729110], [16729111, 20074932], [20074933, 23420754], [23420755, 26766576], [26766577, 30112398], [30112399, 33458220], [33458221, 36804042], [36804043, 40149864], [40149865, 43495686], [43495687, 46841508], [46841509, 50187330], [50187331, 53533152], [53533153, 56878974], [56878975, 60224796], [60224797, 63570618], [63570619, 66916444]] SRR26075333 file size 24721143 SRR26075333 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075333 SRR26075333_1.fastq SRR26075333_2.fastq Input file: SRR26075333_1.fastq Paired file: SRR26075333_2.fastq trimmed: SRR26075333-trimmed-pair1.fastq, SRR26075333-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 20:10:13 2025 >> started Tue Feb 11 20:11:32 2025 >> done (78.833s) 66916444 read pairs processed; of these: 401 ( 0.00%) short read pairs filtered out after trimming by size control 504220 ( 0.75%) empty read pairs filtered out after trimming by size control 66411823 (99.25%) read pairs available; of these: 6694627 (10.08%) trimmed read pairs available after processing 59717196 (89.92%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 43 0.00% 19 48 0.00% 20 56 0.00% 21 80 0.00% 22 73 0.00% 23 135 0.00% 24 119 0.00% 25 108 0.00% 26 121 0.00% 27 135 0.00% 28 173 0.00% 29 130 0.00% 30 174 0.00% 31 131 0.00% 32 151 0.00% 33 176 0.00% 34 174 0.00% 35 186 0.00% 36 172 0.00% 37 202 0.00% 38 228 0.00% 39 244 0.00% 40 218 0.00% 41 273 0.00% 42 278 0.00% 43 287 0.00% 44 273 0.00% 45 321 0.00% 46 310 0.00% 47 316 0.00% 48 332 0.00% 49 399 0.00% 50 400 0.00% 51 385 0.00% 52 487 0.00% 53 436 0.00% 54 425 0.00% 55 532 0.00% 56 513 0.00% 57 568 0.00% 58 664 0.00% 59 625 0.00% 60 643 0.00% 61 784 0.00% 62 800 0.00% 63 793 0.00% 64 1031 0.00% 65 745 0.00% 66 917 0.00% 67 967 0.00% 68 953 0.00% 69 1195 0.00% 70 1185 0.00% 71 1301 0.00% 72 1381 0.00% 73 1563 0.00% 74 1738 0.00% 75 1835 0.00% 76 1872 0.00% 77 2014 0.00% 78 2183 0.00% 79 2375 0.00% 80 2688 0.00% 81 3040 0.00% 82 3328 0.01% 83 3657 0.01% 84 4081 0.01% 85 4441 0.01% 86 4705 0.01% 87 5118 0.01% 88 5472 0.01% 89 6276 0.01% 90 6947 0.01% 91 7599 0.01% 92 8121 0.01% 93 9134 0.01% 94 10528 0.02% 95 11143 0.02% 96 12393 0.02% 97 13611 0.02% 98 14573 0.02% 99 15981 0.02% 100 17629 0.03% 101 18849 0.03% 102 21628 0.03% 103 23229 0.03% 104 25833 0.04% 105 29213 0.04% 106 31194 0.05% 107 34122 0.05% 108 36329 0.05% 109 38238 0.06% 110 40955 0.06% 111 44405 0.07% 112 48621 0.07% 113 52091 0.08% 114 58044 0.09% 115 62586 0.09% 116 66682 0.10% 117 72098 0.11% 118 75106 0.11% 119 79830 0.12% 120 83988 0.13% 121 89509 0.13% 122 96706 0.15% 123 103113 0.16% 124 110144 0.17% 125 117168 0.18% 126 122439 0.18% 127 130901 0.20% 128 136645 0.21% 129 140679 0.21% 130 147076 0.22% 131 152179 0.23% 132 159663 0.24% 133 169247 0.25% 134 171479 0.26% 135 185745 0.28% 136 190053 0.29% 137 196797 0.30% 138 202136 0.30% 139 208382 0.31% 140 215008 0.32% 141 221790 0.33% 142 226712 0.34% 143 232334 0.35% 144 245663 0.37% 145 248761 0.37% 146 255037 0.38% 147 261705 0.39% 148 262906 0.40% 149 269906 0.41% 150 273862 0.41% 151 59717196 89.92% 66411823 reads passed initial QC criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=3.32 fanout-score-rank=24 prefix-density=0.48 prefix-fanout=2.8 sequence=TGGTGATGGGAAGCCAGAAAACTTCCTTGGG criterion=fanout-score sequence-density=0.06 sequence-density-rank=35 fanout-score=69.43 fanout-score-rank=1 prefix-density=0.32 prefix-fanout=13.1 sequence=CAACAGCAACACAAGGCAGCACAACATCCCTTCCAGAAGCCATCACCCCTGGATTTAGTCTTCACAGGAATAGGATTTTGCTGGTCGGATTGACCATCCATGGTTGGATAGCCAGCTGGTGGTGGGCCTGAATATGCACTAGGATAAGTTGATGTTGGTGGGGGAGGATATGCCACTGTAGCTTGATTCTGACTCATGGTTGAAGAAGCGAGTTGAGAAGCACGAACAAATGAGAGCTATGGAAGAATGGAGATAAGGACGAGAGAAGTTCGGTTGTGGT criterion=sequence-density sequence-density=0.44 sequence-density-rank=1 fanout-score=4.11 fanout-score-rank=20 prefix-density=0.62 prefix-fanout=2.9 sequence=AGTATGAGAAAATTAACATGTTCTCTCCAAGTGAAGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=207.96 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=12.3 sequence=AGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAAAGATCAGCACCAGACCACTTAACATCAAAGATATTGACGTCGGAGAGGGGAGCAGTCCTGTTAATAAGTTTCAGAGGTCCATGACTATGCCAGGAACTCCAGGGACACCGACGACACCAGTGACCCCTACAACCCCAGTGTCGGCGCGTAGCAATGTTTGGAGGAGCGTGTTCCACCCTGGTAGCAACCTTGCTACTAAGAATATTGGTGCTCATGTTTTTGACAAGCCACAGCCTAACACACCCACTGTCTATGACTGGATGTACAGTGGAGAGACGAAGAGCGAGCATCGTTGATGAGGTTGCCTTCAACCAAGGTTGCCCATGTAAATACGTACTGTGTTTTGTTTTTCAGTACTCATCTGCAATATGTCTCTTGTTGTTATGGTTCTACGGTTCTACCGTGCCTGGAACA SRR26075333 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 20:12:11 Started mapping on | Feb 11 20:12:11 Finished on | Feb 11 20:20:48 Mapping speed, Million of reads per hour | 462.44 Number of input reads | 66411823 Average input read length | 298 UNIQUE READS: Uniquely mapped reads number | 59913290 Uniquely mapped reads % | 90.21% Average mapped length | 296.62 Number of splices: Total | 49336803 Number of splices: Annotated (sjdb) | 48043142 Number of splices: GT/AG | 48485417 Number of splices: GC/AG | 621988 Number of splices: AT/AC | 51803 Number of splices: Non-canonical | 177595 Mismatch rate per base, % | 0.43% Deletion rate per base | 0.03% Deletion average length | 2.88 Insertion rate per base | 0.02% Insertion average length | 2.62 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1841727 % of reads mapped to multiple loci | 2.77% Number of reads mapped to too many loci | 144425 % of reads mapped to too many loci | 0.22% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.24% % of reads unmapped: other | 0.55% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 4656806 4656806 4656806 N_multimapping 1841727 1841727 1841727 N_noFeature 1589873 59166214 2046217 N_ambiguous 714593 4563 421448 UnstrandedReadsAssigned:57608824 PositiveStrandReadsAssigned:742513 NegativeStrandReadsAssigned:57445625 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075333 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075333-trimmed-pair1.fastq SRR26075333-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 66,411,823 reads, 58,584,133 reads pseudoaligned [quant] estimated average fragment length: 222.98 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,280 rounds 52401 SRR26075333.ke.tsv 34699 SRR26075333.se.tsv 87100 total ==> SRR26075333.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1796.02 10872 76.5123 Potri.005G024800.1.v4.1 1035 813.02 23522 365.684 Potri.004G059700.1.v4.1 961 739.02 56 0.957778 Potri.007G009000.2.v4.1 1416 1194.02 0 0 Potri.003G141000.2.v4.1 2943 2721.02 1819.54 8.45207 Potri.016G087400.1.v4.1 270 81.469 6338 983.315 Potri.015G069301.1.v4.1 564 343.617 0 0 Potri.010G195200.1.v4.1 1773 1551.02 209 1.70318 Potri.012G127500.1.v4.1 977 755.02 41940 702.106 ==> SRR26075333.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 392 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 762 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 7 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1345 SRR26075333 completed mapping pipeline successfully