Starting /dee2/code/volunteer_pipeline.sh SRR26075334 current disk space = 3053070299136 free memory = 1432147852 SRR26075334 SRAfilesize a6899c260792fffb8f78de808f6de7c1 SRR26075334.sra SRR26075334.sra file validated SRR26075334 is paired end SRR26075334 is conventional basespace SRR26075334 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075334_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.525 37.0 37.0 37.0 37.0 37.0 2 36.591 37.0 37.0 37.0 37.0 37.0 3 36.647 37.0 37.0 37.0 37.0 37.0 4 36.7065 37.0 37.0 37.0 37.0 37.0 5 36.697 37.0 37.0 37.0 37.0 37.0 6 36.748 37.0 37.0 37.0 37.0 37.0 7 36.6515 37.0 37.0 37.0 37.0 37.0 8 36.672 37.0 37.0 37.0 37.0 37.0 9 36.6745 37.0 37.0 37.0 37.0 37.0 10-14 36.63855 37.0 37.0 37.0 37.0 37.0 15-19 36.63629999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.568799999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.4931 37.0 37.0 37.0 37.0 37.0 30-34 36.49830000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.411 37.0 37.0 37.0 37.0 37.0 40-44 36.301100000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.0704 37.0 37.0 37.0 37.0 37.0 50-54 36.13440000000001 37.0 37.0 37.0 37.0 37.0 55-59 35.8117 37.0 37.0 37.0 37.0 37.0 60-64 35.7554 37.0 37.0 37.0 37.0 37.0 65-69 35.700100000000006 37.0 37.0 37.0 37.0 37.0 70-74 35.845800000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.0369 37.0 37.0 37.0 37.0 37.0 80-84 36.0826 37.0 37.0 37.0 37.0 37.0 85-89 35.8687 37.0 37.0 37.0 37.0 37.0 90-94 35.9096 37.0 37.0 37.0 37.0 37.0 95-99 35.8318 37.0 37.0 37.0 37.0 37.0 100-104 35.8386 37.0 37.0 37.0 37.0 37.0 105-109 35.6837 37.0 37.0 37.0 37.0 37.0 110-114 35.6322 37.0 37.0 37.0 37.0 37.0 115-119 35.546099999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.5385 37.0 37.0 37.0 37.0 37.0 125-129 35.4662 37.0 37.0 37.0 37.0 37.0 130-134 35.3829 37.0 37.0 37.0 34.6 37.0 135-139 35.229699999999994 37.0 37.0 37.0 29.8 37.0 140-144 35.1298 37.0 37.0 37.0 27.4 37.0 145-149 35.22280000000001 37.0 37.0 37.0 29.8 37.0 150-151 34.99125 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 0.0 20 1.0 21 4.0 22 0.0 23 3.0 24 2.0 25 8.0 26 8.0 27 11.0 28 16.0 29 21.0 30 39.0 31 49.0 32 71.0 33 168.0 34 157.0 35 434.0 36 2821.0 37 185.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.7844611528822 12.93233082706767 7.894736842105263 35.388471177944865 2 17.575 16.1 33.0 33.324999999999996 3 17.549999999999997 18.175 30.8 33.475 4 18.0 27.224999999999998 26.3 28.475 5 24.625 29.15 23.775 22.45 6 24.65 33.15 22.3 19.900000000000002 7 14.799999999999999 30.425 38.574999999999996 16.2 8 17.2 29.775000000000002 30.025000000000002 23.0 9 20.625 24.349999999999998 32.05 22.975 10-14 19.74098704935247 29.53647682384119 26.48132406620331 24.24121206060303 15-19 19.355 27.93 27.689999999999998 25.025 20-24 19.595000000000002 28.955 27.61 23.84 25-29 21.355 28.07 26.565 24.01 30-34 19.220000000000002 28.24 27.85 24.69 35-39 20.86 28.475 26.075 24.59 40-44 19.085 28.58 27.91 24.425 45-49 20.79 27.54 27.66 24.01 50-54 20.75 27.02 27.169999999999998 25.06 55-59 20.855 27.865000000000002 27.21 24.07 60-64 21.349999999999998 27.284999999999997 27.115000000000002 24.25 65-69 20.755000000000003 28.28 26.915 24.05 70-74 22.405 27.465 26.695 23.435 75-79 23.215 26.625 27.11 23.05 80-84 23.185 26.06 27.405 23.35 85-89 22.56 27.534999999999997 26.590000000000003 23.315 90-94 21.88 28.285 26.525 23.31 95-99 22.81 26.825 26.83 23.535 100-104 23.02 26.895000000000003 26.66 23.425 105-109 22.955000000000002 25.995 27.779999999999998 23.27 110-114 22.735 27.435 27.195000000000004 22.634999999999998 115-119 23.205000000000002 26.93 26.205000000000002 23.66 120-124 23.285 25.805 27.395000000000003 23.515 125-129 23.165 26.484999999999996 26.255 24.095 130-134 23.135 27.41 26.105 23.35 135-139 23.835 26.229999999999997 26.19 23.745 140-144 24.04 26.505000000000003 25.645 23.810000000000002 145-149 23.455000000000002 27.245 25.715 23.585 150-151 25.2875 26.85 25.324999999999996 22.537499999999998 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2.0 1 1.5 2 0.5 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 2.5 12 2.5 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 0.5 19 0.0 20 0.5 21 0.5 22 0.0 23 1.0 24 2.5 25 5.0 26 6.0 27 8.0 28 6.5 29 5.5 30 13.0 31 15.0 32 22.0 33 32.0 34 40.0 35 58.0 36 66.5 37 76.5 38 109.5 39 141.5 40 183.5 41 209.0 42 229.5 43 260.0 44 247.5 45 258.5 46 267.5 47 251.5 48 244.5 49 203.0 50 177.5 51 158.0 52 111.5 53 84.5 54 64.5 55 61.0 56 66.5 57 56.5 58 41.0 59 25.0 60 17.0 61 13.0 62 8.0 63 9.5 64 14.5 65 13.0 66 16.0 67 25.5 68 24.5 69 17.5 70 11.5 71 5.0 72 3.0 73 1.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.25 #Duplication Level Percentage of deduplicated Percentage of total 1 64.43983402489627 38.824999999999996 2 20.456431535269708 24.65 3 7.800829875518672 14.099999999999998 4 3.6929460580912865 8.9 5 2.323651452282158 7.000000000000001 6 0.5394190871369294 1.95 7 0.4564315352697096 1.925 8 0.16597510373443983 0.8 9 0.04149377593360996 0.22499999999999998 >10 0.04149377593360996 0.25 >50 0.04149377593360996 1.375 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTGTTATCTCGTAT 55 1.375 TruSeq Adapter, Index 5 (97% over 39bp) CGCGACAACAACCTTGCTCTTGGGTGTGCCATTTTGCCTGCCTTCAGCTT 10 0.25 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTGTTATCTCGTTT 9 0.22499999999999998 TruSeq Adapter, Index 5 (97% over 39bp) CCCAATTTCAGCATTCTGTGGATGCGACTGCCAAAGGTGTTGGGATCATC 8 0.2 No Hit TTTTTTTTAAGATCAACACAGATGATATAATCATCAATCATTGGACATAG 8 0.2 No Hit GCTCATAAATCATTGAATTCGCTTTCATATATGATTATTTGGCTAATGGA 8 0.2 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTGTTATCGCGTAT 8 0.2 TruSeq Adapter, Index 5 (97% over 39bp) GTCAACTTCTCCCTATCTTGAAAAAAAAAATACTGATACATACACAAACA 7 0.17500000000000002 No Hit CCCCAATGAAGCCCCAAGCATTTCTTCCCCAAATCGAAGATCAAATGCTG 7 0.17500000000000002 No Hit GCTAGTATTATCTGACCTTGATCTCGGCATTGGATAACCTCAAGCAATGT 7 0.17500000000000002 No Hit CAGTGTCGTAGTTCCTCCATCAACACGAGGACTGCAAGTACTCGGTGAAA 7 0.17500000000000002 No Hit GCCAATGTCCCACTGACCCATCATTTAGGGCAGTATCCCCAGCCCTATGC 7 0.17500000000000002 No Hit CCTTGTTCTTGTTGGTTCTTAAGGCGGGAACTCTTCATTCCATATGGATA 7 0.17500000000000002 No Hit GATCGATGGAGTTTCTCAAAGAGAGTAGGTTTCTTCTCCTCTTCCTCCTT 7 0.17500000000000002 No Hit GGGTTATCCAAAGGTCTCGGAGAGGGTCATAAAGCTGCCACAAATTCTCA 7 0.17500000000000002 No Hit CTCAGCGAGTCCTCACTTTGCTGATGCCAAGAATATTTACAGCAGTTCCT 7 0.17500000000000002 No Hit CCTAGCTCTCGAATTCCTCTTCAACACATACTCCTCGTCATAAGATCTCG 7 0.17500000000000002 No Hit CAGCAATCAAAACCTCATCATTTTCCTCAATGTAGTTTAAGCAACCATCA 7 0.17500000000000002 No Hit GAAGCCTTGTATGTTGTCTTGAGCTTCCTGGATGGGGGTCTAACCTTCTT 6 0.15 No Hit GCTTCAGCTGCTGCAACTCCTGGCTACTTTCTTCAAACTCTTTTTGACGC 6 0.15 No Hit GCACCCTGGCCAGGGCCGGTACCATCAAGATCCTTTTCAGTGGGACGATA 6 0.15 No Hit CAAAGCATCAGTGAGATCACGACCAGCAAGATCCAAACGAAGGATGGCAT 6 0.15 No Hit TGGCAAGCTTCTCCACCAGATGGTAATCCTCAAGGAGGATTGGACCTCTA 6 0.15 No Hit GGCGAGCATGTGGGCGCAGGCACTGAGCGCCGAGCGCCGGTAGCGAGTAT 6 0.15 No Hit CCTCATCTCTAGCTGTTAAAACCACCAAAACCCCTTTAGATGCCAACTGC 6 0.15 No Hit ATTCAACCATTCCTGTAAGATTCATGTCAATCATCATCAGAGCCAAGGAC 6 0.15 No Hit GTCCTTGGATCATGGTAAGGTGCAGTTGGGTTCATCAGGAGGGCAATTCT 6 0.15 No Hit ATTGTAATATGAATAAGCAGCTTTCAATGTCGAGTGGTTCTGGTTGCCAA 6 0.15 No Hit GCCATTATTATTACTCCTCCTATGCACCCTGAAGATGAAAAGATGGGGTT 6 0.15 No Hit CTCTCTCCTCATCCCAAATTTCCATGCTTGCCAGGAACAACCACCATCGG 6 0.15 No Hit CCATTGATGAGAGACGAATGGGCAAATGGGCAAACATGTCAAGTCTCCGA 6 0.15 No Hit AGTCTACAAGGTACTAATGGTTTTGAACTGAGCGTCCACATGCATCCACC 5 0.125 No Hit ACCTCTTTAGTCTTTTTCGCCTCTGGAAGCTTGTAAGATGCCCTGATCTT 5 0.125 No Hit CTCCGGTAAGATGCCTCTTAACGAATGTGGTCTTTCCTGTACCACCATCA 5 0.125 No Hit CATCAAAAACCAAACAATTTAGGTTGGGAAGTAGAGCAGAACAGACTGTT 5 0.125 No Hit CCTTGCTCTTGGCCTGGGATTTGGAACCCATGATTCCACCACCCCACTTC 5 0.125 No Hit TTTTGGAGGACACAACCATTGCAAAAACTCCAGAACATCCATTCTCCAGG 5 0.125 No Hit GCCACTGCATATTCGCCCCATGTATGGCATTGGCCACCTCATCAAACACC 5 0.125 No Hit CTCTGCTGCCTGAGTTTGTAGCTGGGAACTCAGGTCTTCGATCTTAGCCT 5 0.125 No Hit ACGGAAGGTGGTTGTTTCCTCTGAGTGTACTTTGGAGGAGGATAATCGGG 5 0.125 No Hit GTCATCACTGTTTTCTCCACTTCCATAAGAAACTGGAATCCTCACTGCAT 5 0.125 No Hit TTACAGGAGATACATCAGGTGTCAGCAAGCAAAACCGGAGACGATAGATG 5 0.125 No Hit GGGGTGTGAAGGGGTTTTAAAGGAGTAGAACTGTGCATGAATCAGAAAGT 5 0.125 No Hit CAATCCGTGAACGCCCTCCAATATTACAGTCGCAGGAAAAGCATTAGAAT 5 0.125 No Hit TATCTTTTGATGGATTTGCTCCCCCCTTCAACAACCACCACACAAATTCC 5 0.125 No Hit ATACATCTCGACAGATTTCTCAGCAAAGTTGTTCAACTGGCCAAGAAATC 5 0.125 No Hit GCTGAATGCGAACGGGTCCCACGTGCCGAACCCCATATCCAGCGTGACGT 5 0.125 No Hit GCAGATATCTGTACAACCCTTCGGCTGCCAACTCGTGTTAAGGCCAAGAG 5 0.125 No Hit ATCACAAACTCCCTACTTCTTCTTTGCAGCGTCTCCAGCCTTTGTCTTCT 5 0.125 No Hit CAAGACGAGCTGCTACTACATTGACAGCCTGATGACGTAGCATGTCCTGC 5 0.125 No Hit GGTTCAAAGGCTGTATATTTGTACTCTTCAGAGGCATCTCATGGTTATGT 5 0.125 No Hit ACCATTCATCAATCCCAACACCACTCCACCTCATCTCCAAAATACCGGTT 5 0.125 No Hit TTCATCACATACTTCCCCAAGCCCTCATCATTACCAAAGCTCAAGCCACA 5 0.125 No Hit AGGATTTGTTTCCTTCCATCACCAAATGGAAGGGCTAACCAAGGCATTTC 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTGTTATCTCGGAT 5 0.125 TruSeq Adapter, Index 5 (97% over 39bp) GCTTCATTCACGGTGATGTTACGCCCATCAAGGTCTTGGCCGTTCATTCC 5 0.125 No Hit CTCTTTTATCAGCCACACGCTGCCCAACAATCTCACCCACATACTCCACC 5 0.125 No Hit TTCACAATCAAGCTGCAACGACAGGATATGATGCACAGGTTGCAATACCA 5 0.125 No Hit GTCTTCAGCAAGCAGTCAAATGTGGCCTTAACAAAGTTTCCAAGAGTCTT 5 0.125 No Hit CGTTGTTAAACAGCTAAAACACACACTAGGATACAGTACTGCTTCGTCGC 5 0.125 No Hit CTCTTGTAGTGCAGTTGCTGCACACGGTTCGGTACACGAGTACGCCACAG 5 0.125 No Hit CCAACACCTTCTCAGACGCCACCTCGGAGAACACCTTCTCACAATTCGAA 5 0.125 No Hit CCCACAACATGAAGAATAGCAACAAAAGCAATGAAACCAATGCTCATAAC 5 0.125 No Hit GCTCCATCGAGTGTCCTGGCTTTGCTTCTAAGGTCCATACCAGAACCACC 5 0.125 No Hit GCCTTAAAGAGTTTGAGATTCTCCGTTGCCTGGTCAATTTTCATCAAGTG 5 0.125 No Hit ATTACGAATAACCTTAGCTTGAACAATCTCTCCAGTTGCAGAGAAGATGC 5 0.125 No Hit TCAATGAAGGGCAAGAGTCTCTGGGTTCCTGGAGGAAGAGGGGATGAAGA 5 0.125 No Hit GTGTGAAAGGCGGGTATATGCCGTAGCGTGTTGGTACATGTATACGAAGT 5 0.125 No Hit CATGGTTGTCCATGGAAGTTAGCAGTGACCTACTCTTGATCTCAGTATAA 5 0.125 No Hit CTCTCATTTACAAGTGCAAGGATCGCAGGTACAGTTGGCTCCACACTTGC 5 0.125 No Hit CAAACATAAAAATTCTAACAAGATCATACTTGTTCCAGACCCATCTGCTT 5 0.125 No Hit CTCCAGAAAAAAGCACTAGAACAACTGGATTTTTGGCTGCTTTTGCAACA 5 0.125 No Hit GTCACCACTTTGTTTTGCCTTCTCAACACTCTTCTGCAGGGTTTCTGTAA 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTGTTATCGCGTTT 5 0.125 TruSeq Adapter, Index 5 (97% over 39bp) GTCATGAGATCCCGATGCAATGACATTTCCAGCTGGATTGAACTTCATAG 5 0.125 No Hit CTCTGCTGCAGCACGACTTACAAACTCAATAAAACCATAACCCTCTGGAT 5 0.125 No Hit AGGGCTTAAGCACGTTCACCACGGATACGCCTAGCAAGCTGGATGTCCTT 5 0.125 No Hit GTCCAATTAACTGATTTTGCGACATGTTAGTGGTATTTGATTGTTGCCCC 5 0.125 No Hit CTGCTCTTCTACAAGCTCCTTGATGCTGTCAAGTTCTCCATGTTCATGAT 5 0.125 No Hit GCTTTTCTAAGGAAGGAGTTATACTCCTTTGAGTAAGGAACACTATATAG 5 0.125 No Hit AGCCCTTATGTTGTACAACTCCTCAAGAGGCTTCTCGATGTGCATCGCCT 5 0.125 No Hit GGCAACATAAGACCATTGTTATGAGCATATATAATACCTTCTCGTCTGTT 5 0.125 No Hit CTTCAACCTTCTCCTCTTTCTTTGCCTCGGCAGCAGGGGCAGCACCACCG 5 0.125 No Hit CGCAAAAAGGATTCGAAGCTAAGCACCCAAAAGAAACAGTTTTGTCAACG 5 0.125 No Hit CACATTCTTTCTGGTTCTCTGAACCTTCTTTTTACCGGCTTCTTGCTCTG 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCAGTGTTATCGCGGAT 5 0.125 TruSeq Adapter, Index 5 (97% over 39bp) GCCTGCACAATCAAAAGTGACATCCACTCCAGTCCCCATAGCTTGATGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.05 0.0 0.0 0.0 0.0 2 0.05 0.0 0.0 0.0 0.0 3 0.05 0.0 0.0 0.0 0.0 4 0.05 0.0 0.0 0.0 0.0 5 0.05 0.0 0.0 0.0 0.0 6 0.05 0.0 0.0 0.0 0.0 7 0.05 0.0 0.0 0.0 0.0 8 0.05 0.0 0.0 0.0 0.0 9 0.05 0.0 0.0 0.0 0.0 10-11 0.05 0.0 0.0 0.0 0.0 12-13 0.05 0.0 0.0 0.0 0.0 14-15 0.05 0.0 0.0 0.0 0.0 16-17 0.05 0.0 0.0 0.0 0.0 18-19 0.05 0.0 0.0 0.0 0.0 20-21 0.05 0.0 0.0 0.0 0.0 22-23 0.05 0.0 0.0 0.0 0.0 24-25 0.05 0.0 0.0 0.0 0.0 26-27 0.05 0.0 0.0 0.0 0.0 28-29 0.05 0.0 0.0 0.0 0.0 30-31 0.05 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.125 0.0 0.0 0.0 0.0 68-69 0.125 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.15 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.1875 0.0 0.0 0.0 0.0 82-83 0.21250000000000002 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.2875 0.0 0.0 0.0 0.0 88-89 0.44999999999999996 0.0 0.0 0.0 0.0 90-91 0.475 0.0 0.0 0.0 0.0 92-93 0.5625 0.0 0.0 0.0 0.0 94-95 0.575 0.0 0.0 0.0 0.0 96-97 0.625 0.0 0.0 0.0 0.0 98-99 0.75 0.0 0.0 0.0 0.0 100-101 0.8 0.0 0.0 0.0 0.0 102-103 0.85 0.0 0.0 0.0 0.0 104-105 1.1124999999999998 0.0 0.0 0.0 0.0 106-107 1.2875 0.0 0.0 0.0 0.0 108-109 1.5499999999999998 0.0 0.0 0.0 0.0 110-111 1.7625000000000002 0.0 0.0 0.0 0.0 112-113 1.875 0.0 0.0 0.0 0.0 114-115 2.075 0.0 0.0 0.0 0.0 116-117 2.3375 0.0 0.0 0.0 0.0 118-119 2.575 0.0 0.0 0.0 0.0 120-121 3.0375 0.0 0.0 0.0 0.0 122-123 3.5 0.0 0.0 0.0 0.0 124-125 3.7750000000000004 0.0 0.0 0.0 0.0 126-127 4.1125 0.0 0.0 0.0 0.0 128-129 4.7 0.0 0.0 0.0 0.0 130-131 5.2 0.0 0.0 0.0 0.0 132-133 5.9125 0.0 0.0 0.0 0.0 134-135 6.7125 0.0 0.0 0.0 0.0 136-137 7.35 0.0 0.0 0.0 0.0 138-139 8.1875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTGAAG 10 0.006830828 145.0 5 TTTTGCC 10 0.006830828 145.0 7 TCCTTAG 10 0.006830828 145.0 4 GCTAGTA 10 0.006830828 145.0 1 GATATAA 10 0.006830828 145.0 145 GTATTAT 10 0.006830828 145.0 5 AGTATTA 10 0.006830828 145.0 4 ATTATCT 10 0.006830828 145.0 7 TGAAGGG 10 0.006830828 145.0 7 CTAGTAT 10 0.006830828 145.0 2 TATTATC 10 0.006830828 145.0 6 GGGGTGT 10 0.006830828 145.0 1 GCCAAAG 10 0.006830828 145.0 2 GGGTGTG 10 0.006830828 145.0 2 GTGTGAA 10 0.006830828 145.0 4 GGTGTGA 10 0.006830828 145.0 3 GAGCACA 30 0.0017973486 72.5 9 AGAGCAC 30 0.0017973486 72.5 8 GATCGGA 35 0.0033124194 62.14286 1 TCGGAAG 35 0.0033124194 62.14286 3 >>END_MODULE SRR26075334 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075334_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.9345 37.0 37.0 37.0 37.0 37.0 2 36.084 37.0 37.0 37.0 37.0 37.0 3 36.2885 37.0 37.0 37.0 37.0 37.0 4 36.1635 37.0 37.0 37.0 37.0 37.0 5 36.1555 37.0 37.0 37.0 37.0 37.0 6 35.986 37.0 37.0 37.0 37.0 37.0 7 36.0945 37.0 37.0 37.0 37.0 37.0 8 36.0385 37.0 37.0 37.0 37.0 37.0 9 36.2105 37.0 37.0 37.0 37.0 37.0 10-14 35.909 37.0 37.0 37.0 37.0 37.0 15-19 35.8451 37.0 37.0 37.0 37.0 37.0 20-24 35.71 37.0 37.0 37.0 37.0 37.0 25-29 35.471399999999996 37.0 37.0 37.0 37.0 37.0 30-34 35.27289999999999 37.0 37.0 37.0 37.0 37.0 35-39 35.221199999999996 37.0 37.0 37.0 37.0 37.0 40-44 35.1265 37.0 37.0 37.0 37.0 37.0 45-49 35.084700000000005 37.0 37.0 37.0 37.0 37.0 50-54 34.83630000000001 37.0 37.0 37.0 27.4 37.0 55-59 34.9178 37.0 37.0 37.0 25.0 37.0 60-64 35.0231 37.0 37.0 37.0 27.4 37.0 65-69 34.8985 37.0 37.0 37.0 27.4 37.0 70-74 34.772000000000006 37.0 37.0 37.0 25.0 37.0 75-79 34.679899999999996 37.0 37.0 37.0 25.0 37.0 80-84 34.7904 37.0 37.0 37.0 25.0 37.0 85-89 34.823299999999996 37.0 37.0 37.0 25.0 37.0 90-94 34.7928 37.0 37.0 37.0 25.0 37.0 95-99 34.9125 37.0 37.0 37.0 25.0 37.0 100-104 34.9446 37.0 37.0 37.0 27.4 37.0 105-109 34.8788 37.0 37.0 37.0 27.4 37.0 110-114 34.9144 37.0 37.0 37.0 25.0 37.0 115-119 34.8888 37.0 37.0 37.0 25.0 37.0 120-124 34.8153 37.0 37.0 37.0 25.0 37.0 125-129 34.793400000000005 37.0 37.0 37.0 25.0 37.0 130-134 34.751400000000004 37.0 37.0 37.0 25.0 37.0 135-139 34.5731 37.0 37.0 37.0 25.0 37.0 140-144 34.5965 37.0 37.0 37.0 25.0 37.0 145-149 34.5594 37.0 37.0 37.0 25.0 37.0 150-151 34.28175 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 11.0 14 12.0 15 13.0 16 11.0 17 12.0 18 8.0 19 11.0 20 10.0 21 22.0 22 16.0 23 13.0 24 24.0 25 31.0 26 41.0 27 23.0 28 22.0 29 28.0 30 26.0 31 38.0 32 60.0 33 103.0 34 194.0 35 669.0 36 2434.0 37 167.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.400000000000006 21.475 10.299999999999999 21.825 2 29.975 24.675 25.924999999999997 19.425 3 25.2 28.825 28.025 17.95 4 27.425 30.875000000000004 22.650000000000002 19.05 5 30.425 32.975 19.3 17.299999999999997 6 26.75 35.85 20.05 17.349999999999998 7 26.200000000000003 23.1 31.45 19.25 8 25.575 26.474999999999998 24.15 23.799999999999997 9 24.8 25.8 26.1 23.3 10-14 27.325 28.1 24.055 20.52 15-19 27.27 27.265 24.965 20.5 20-24 26.334999999999997 28.155 25.135 20.375 25-29 26.86 27.005000000000003 25.230000000000004 20.905 30-34 27.200000000000003 27.16 25.374999999999996 20.265 35-39 26.46 27.67 24.88 20.990000000000002 40-44 25.785000000000004 27.765 26.105 20.345 45-49 26.8 26.805 25.5 20.895 50-54 23.785 27.589999999999996 28.46 20.165 55-59 25.679999999999996 27.595 26.605 20.119999999999997 60-64 26.52 28.205000000000002 25.924999999999997 19.35 65-69 26.125 27.800000000000004 25.39 20.685000000000002 70-74 24.645 28.744999999999997 26.284999999999997 20.325 75-79 24.945 28.99 25.535000000000004 20.53 80-84 26.255 28.515 25.590000000000003 19.64 85-89 26.365 28.275 25.495 19.865 90-94 26.685 27.845 25.66 19.81 95-99 25.865 28.01 25.69 20.435 100-104 26.215 27.955000000000002 25.205 20.625 105-109 26.224999999999998 28.599999999999998 25.705 19.470000000000002 110-114 27.015 27.634999999999998 25.31 20.04 115-119 25.865 28.115000000000002 26.045 19.975 120-124 26.805 27.72 26.105 19.37 125-129 26.150000000000002 27.985 26.169999999999998 19.695 130-134 26.87 26.590000000000003 26.3 20.24 135-139 27.965 26.85 26.0 19.185 140-144 28.470000000000002 27.105 25.665 18.759999999999998 145-149 27.595 28.444999999999997 24.709999999999997 19.25 150-151 30.625000000000004 26.687499999999996 24.775 17.9125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 2.0 8 2.0 9 0.5 10 1.0 11 2.0 12 2.0 13 2.5 14 2.0 15 1.5 16 1.5 17 1.0 18 1.5 19 2.0 20 2.5 21 2.5 22 2.5 23 1.5 24 1.0 25 2.5 26 3.5 27 4.0 28 4.0 29 7.0 30 13.5 31 12.5 32 15.0 33 26.5 34 28.0 35 32.0 36 36.5 37 49.5 38 87.0 39 136.0 40 176.5 41 205.5 42 232.5 43 250.5 44 262.5 45 264.5 46 262.5 47 258.0 48 250.5 49 236.0 50 210.0 51 154.0 52 108.0 53 96.5 54 82.5 55 65.0 56 49.5 57 42.5 58 32.5 59 26.5 60 24.5 61 19.5 62 15.5 63 11.0 64 5.5 65 4.0 66 4.0 67 1.5 68 0.5 69 3.5 70 3.5 71 2.0 72 2.5 73 2.0 74 3.5 75 3.0 76 1.5 77 3.0 78 6.0 79 6.0 80 5.0 81 6.5 82 6.5 83 7.5 84 11.0 85 10.0 86 6.0 87 5.5 88 9.0 89 11.0 90 5.0 91 0.5 92 1.0 93 2.5 94 4.0 95 3.0 96 3.0 97 3.5 98 2.5 99 1.0 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.175000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 68.18361693707953 43.075 2 18.20340324495449 23.0 3 7.439651760981401 14.099999999999998 4 3.126236644242184 7.9 5 1.9786307874950535 6.25 6 0.4352987732489117 1.6500000000000001 7 0.4352987732489117 1.925 8 0.07914523149980214 0.4 9 0.0 0.0 >10 0.1187178472497032 1.7000000000000002 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 34 0.8500000000000001 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 23 0.575 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT 11 0.27499999999999997 No Hit CAATGGAGATAAATCCAGAGAACCCAATCATGGATGAGCTGAGGAAGAGG 8 0.2 No Hit GCACGTTTCAGGTGTTGCATGACTCAACCCATGACAGTTGACTGATCCAG 8 0.2 No Hit AGAGGGGCTTATGAGGTTCTTCGAGATGGGGTGGGCGTAGATAAGTTCTG 7 0.17500000000000002 No Hit CGAATGTCCATTTTGTGGTGAATTGATGATCCGTCAGATCTCTTTGCCTT 7 0.17500000000000002 No Hit CTTCTCAAAGCCCTAGCAAACAACGGCCTAATCGACCCAGCTTCGCACCG 7 0.17500000000000002 No Hit ACTTCGGCCGGGCCAAGAGGCGGCCACCAGTTATGAAGGTTCCCTATGCT 7 0.17500000000000002 No Hit GTAGTTGGAACATTTGGTAGAGCAATGGTAGAGATGATGATGATGATGAT 7 0.17500000000000002 No Hit AGAAACTATATGAAACTGATGCAACATTGGTTAGTATGTCTGAACTGATT 7 0.17500000000000002 No Hit GCAAATCACTCGAAGCAGAAGCTTACTCATTTTAATTACTCTGTTGTGAT 7 0.17500000000000002 No Hit TGGACAGAGCTCATATAACAGTGTGATGCGACTGAATGGTGGATTCCCTG 7 0.17500000000000002 No Hit AACAAGACTTTGAGACGGACATCGAAGGTAACGAGGTGTCTGAACTTGGG 7 0.17500000000000002 No Hit GGCATGGAGGCCGCTGCTGGCCGATGCGACCCCCTTCCATCTGCATCCTC 7 0.17500000000000002 No Hit GAATAAGACTTTATAGGTTTGTGGGAGTGAAGGCGGTTTTTAGTGTAGGT 7 0.17500000000000002 No Hit CCGCCTCTGCGCTATCTCTGGGTGTAAGAAGAAAAAGAAATAAAAAGAAG 6 0.15 No Hit GTTCGAATAGAGCAACGGAAGCTGGTTATTGGAAGGCTACAGGGAAGGAT 6 0.15 No Hit TGCAATGTATGTTGCTATCCAGGCTGTCCTTTCCCTGTATGCCAGTGGTC 6 0.15 No Hit GCAACAGGGGATTCTGAACTGAGAGATATAGAAGAGGAAGAAGATGGAAG 6 0.15 No Hit GCCTGCTGTCACGAGGTTGCTAAGAAATTACTTGAGCCAGTTTGTTGTTG 6 0.15 No Hit AGATCGCTTGTGGACATTGTCCCTCCTCTTCGTTGTGTACACCCCTGTAC 6 0.15 No Hit CGCTGAGTTCTTATTCTAGCTAGTGGTTTAATAAGGATGTTATGTTAATG 6 0.15 No Hit TCTAGGTTCTGTTGAAATTCTTCTCAGTCGGCATTGTCCAATTTGGTATG 6 0.15 No Hit GCCAAAGCTTTCACCAGGAAAACATGATGTTGAAGGAACAAATGCAAGCA 6 0.15 No Hit GAGCCGAACTGGCTATCACAACATGTACAAGGAATACCGCGACACAACTC 6 0.15 No Hit TATAAGTTGTCCCAACAATTATCTCGGTAGCATCACACAATCTGAAGCTC 6 0.15 No Hit GTCCTTCACCCTTCTGGTGTCAAGAAGATGGGTGAGTTACATCTAGCCAT 5 0.125 No Hit CAGTCTCTAGCTATTTGCTCTCAGCCCCTCATCCTAAAAAAACAATGAAG 5 0.125 No Hit GGTACTGGGGGAACAAGATCGGAAAGCCACATACTGTGCCTTGTAAGGTT 5 0.125 No Hit GACGAGAAAGGCTAGAAACTGTATGTAGCTATGGCAAGCATGATCATATC 5 0.125 No Hit ATGGAAGACAGAGAAGCTGGATTACAAGACAGATTGAGCGATCTACCCGA 5 0.125 No Hit GCAAACCCTTACTTCTCAAGCTCTTGGCGGCGGAATTACACGGGGGGTTT 5 0.125 No Hit CAAAAGTGGCTCCAAGGTCCCAGTGTCCGATCTAGTTGGAAAGAACATTC 5 0.125 No Hit GTTTGCGTGACAGGTGCTGGAGGGTACCTGGCTTCTTGGGTCGTCAAGCT 5 0.125 No Hit GTTTTTGGCAGACTGATTTGAGGTTCCAGAGCCATGCTGTCCTTGCACTC 5 0.125 No Hit GAGTGAAGCTGACATGGTATTTTTACATTTGAAATCATTGCAGACAGACA 5 0.125 No Hit CATGCACTGGGCTTTCTAAGTTGCCAATGTCAGATGTGGAGCATGATTGT 5 0.125 No Hit GGTGGGCTGACAAGTCATACAAGAAATCCAACCTTGGAAATGAGTGGAAG 5 0.125 No Hit CCATAGCAAAGTACATGGAAGAGAAGCACAAAGCAGTACTCCCAGCAAAT 5 0.125 No Hit AGGAGATGGAGACGAGATTAAGTCGTTGTGGATCGGGGATTTGCAGCAGT 5 0.125 No Hit GAGAAATGCAAACCTGGTCGCATCAGGGGTGGCCAGAAACATGCGAAGAG 5 0.125 No Hit GAGTCAGAAAGTAAAAGGCTTTCAACGTTGAGCTCTCTGCTTTTCCTTTT 5 0.125 No Hit GTTCATACCAAAAAAATTCTAATGGGAAACGGTTTTGGTAAACTGACGGC 5 0.125 No Hit AAGTGCTTTGTCTGTCTCCGGTCCAAGGCCAATGGAGTGGTCTACTGTTC 5 0.125 No Hit GTCTGAACTTGACTCCTTATCTGATTTTCTTGGACTTGATCATCGTTCTA 5 0.125 No Hit TCACTTTTTGCCACGCCAAGAGAAGAACCAAGGGAAGAAATCTAAATTCT 5 0.125 No Hit GTTAGCATACCTGCTACCAAGCATGCTAGTTCTCCCCTGTTGTCCAACAA 5 0.125 No Hit GAATGTTCGAGCTGATGTTCCAAATGGGGTCTACCATCCTTCAATGAAAA 5 0.125 No Hit AACACACCGTATCCCTCACAATTTCTCTCTCTTTCTGAAATCCAAAATGG 5 0.125 No Hit GAAGAGGATGATGATGAGGATGATGAAGACGACGAAGATGACAAGGATGA 5 0.125 No Hit AATTAACCCTTCGTCTTTATCAAAAATATCGTAGGGTTTCTCAATGGCTG 5 0.125 No Hit GCCTGACTCACCAGCAAAGGATAGTTCCACATACAAGCCTGGTGCTACTA 5 0.125 No Hit CTGCAAATTCTGTGCTTCGCTTTCTGTTTTTCAATAATAATAATAATTAG 5 0.125 No Hit GTGGGTGTGGCTCTGGCTGCAAGTGCGGCAGCGGCTGTGGAGGATGCAAG 5 0.125 No Hit AAATGGCTGTAGGCGGAACTGCTCCCCCAAGAGGAAGTGCAGCAGCAGCT 5 0.125 No Hit CCTATTGCTCCTGTAAGCTCTCTTAGCTGATGCCTTTTCCTGCGACTGTA 5 0.125 No Hit ACACAATCCTCCCTTGCTTAAAACGACATTGGCCACAGCTCTCCTCGTCC 5 0.125 No Hit AGGATGAGATCAGGTGGTTGAAGAATTGTAAGCACATTTTTCACAGGGCT 5 0.125 No Hit TGATGAAGTGAGCAATGCCAAGAATCTAAAAAATGAGCTCCATAACACCC 5 0.125 No Hit GTCTGGACCAAACTCAGGAGAGGGAAGAGCTTGATCGCACTGATTTGTTG 5 0.125 No Hit CAACCAGATCAACAACAACAATACCAACAACAACAGCAGCAATGGATGAT 5 0.125 No Hit ACACAACCAATGCTCCAGCAACATCCACAATCAGTCCTCAGGCAGCAGCA 5 0.125 No Hit CTATTTTGAAAAATTAACCCAAAATGGCTTTGCCGAATCAGCAAACTGTT 5 0.125 No Hit AAGAATTCCGTATGCTCGAGCTGTCAGTGCTCGAGATCAACTGATCGTTG 5 0.125 No Hit GCTCAGTGCATGGATTCCAACCATGTACATGGCATCCCCGTCAGCTTCGT 5 0.125 No Hit GCAAGAAATCCCCCAATTTGTCATTGACGAGTGCTATGACATGGAGCTGT 5 0.125 No Hit GTTTCTAAGGCAGAATTGAAGGAGAAGCTGGCAAGTTTGTATGAGGTGAA 5 0.125 No Hit AATCAATATGTTAAAGAAGACCGAAATGGTGCAGTGGCGGTGGAAGAAAG 5 0.125 No Hit GGTATGCAAGTGCGACTCATATGCCACCTTCCGTTCTTAGCCGTGGTGTT 5 0.125 No Hit AAAGGATGGGTCACTCTAATGTCTGGAACTCTCACCCCAAGAACTACGGC 5 0.125 No Hit AGAGAAAGCTCTTTGATGACCCAAATACTGTGGAAACTATTGTTTCTATT 5 0.125 No Hit AGTTTCCGTCTGTACAAGGAAGGAGTTTACACCACCAGCACTTGTGGCAG 5 0.125 No Hit TGAACGTGGAGCACAATGGGGAACAGGAGGAGCAAGCTACCATGTGGAAC 5 0.125 No Hit CTTTTCCTGCAGAGAGTCCTTTCGGTTTCGAAGGAGGTCTACATCGGATG 5 0.125 No Hit GTTCGATGATTAATAGCCAAGAGTTTAGTGGTACGGAGATTGTTAAGGCT 5 0.125 No Hit GAAAGAACAAAATGATGCAATGGGCTTTTTCACCGTCATCAAAAGACACA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.025 0.0 0.0 0.0 0.0 44-45 0.025 0.0 0.0 0.0 0.0 46-47 0.025 0.0 0.0 0.0 0.0 48-49 0.025 0.0 0.0 0.0 0.0 50-51 0.025 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.1125 0.0 0.0 0.0 0.0 82-83 0.1375 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.2125 0.0 0.0 0.0 0.0 88-89 0.30000000000000004 0.0 0.0 0.0 0.0 90-91 0.325 0.0 0.0 0.0 0.0 92-93 0.4125 0.0 0.0 0.0 0.0 94-95 0.425 0.0 0.0 0.0 0.0 96-97 0.475 0.0 0.0 0.0 0.0 98-99 0.6 0.0 0.0 0.0 0.0 100-101 0.6625 0.0 0.0 0.0 0.0 102-103 0.725 0.0 0.0 0.0 0.0 104-105 0.9750000000000001 0.0 0.0 0.0 0.0 106-107 1.1375000000000002 0.0 0.0 0.0 0.0 108-109 1.4125 0.0 0.0 0.0 0.0 110-111 1.6375000000000002 0.0 0.0 0.0 0.0 112-113 1.7625000000000002 0.0 0.0 0.0 0.0 114-115 1.975 0.0 0.0 0.0 0.0 116-117 2.25 0.0 0.0 0.0 0.0 118-119 2.5 0.0 0.0 0.0 0.0 120-121 2.925 0.0 0.0 0.0 0.0 122-123 3.4000000000000004 0.0 0.0 0.0 0.0 124-125 3.7249999999999996 0.0 0.0 0.0 0.0 126-127 4.0625 0.0 0.0 0.0 0.0 128-129 4.6625 0.0 0.0 0.0 0.0 130-131 5.2875 0.0 0.0 0.0 0.0 132-133 6.025 0.0 0.0 0.0 0.0 134-135 6.8125 0.0 0.0 0.0 0.0 136-137 7.4625 0.0 0.0 0.0 0.0 138-139 8.3 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCAGA 10 0.006830828 145.0 5 GGCCGGG 10 0.006830828 145.0 6 TCGGCCG 10 0.006830828 145.0 4 TAGCTGA 10 0.006830828 145.0 6 ACTTCGG 10 0.006830828 145.0 1 GTTTAGC 10 0.006830828 145.0 6 GCAGTAG 10 0.006830828 145.0 2 GCCGGGC 10 0.006830828 145.0 7 ATGCAAA 10 0.006830828 145.0 6 CGGCCGG 10 0.006830828 145.0 5 CCGGGCC 10 0.006830828 145.0 8 AATGCAA 10 0.006830828 145.0 5 TTTATAT 10 0.006830828 145.0 145 CAAACCT 10 0.006830828 145.0 9 TTCGGCC 10 0.006830828 145.0 3 >>END_MODULE Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995640 spots for SRR26075334.sra Written 3995640 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra Read 3995626 spots for SRR26075334.sra Written 3995626 spots for SRR26075334.sra SRR ids: ['SRR26075334.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_zfeg91ur SRR26075334.sra spots: 79912534 blocks: [[1, 3995626], [3995627, 7991252], [7991253, 11986878], [11986879, 15982504], [15982505, 19978130], [19978131, 23973756], [23973757, 27969382], [27969383, 31965008], [31965009, 35960634], [35960635, 39956260], [39956261, 43951886], [43951887, 47947512], [47947513, 51943138], [51943139, 55938764], [55938765, 59934390], [59934391, 63930016], [63930017, 67925642], [67925643, 71921268], [71921269, 75916894], [75916895, 79912534]] SRR26075334 file size 29524416 SRR26075334 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075334 SRR26075334_1.fastq SRR26075334_2.fastq Input file: SRR26075334_1.fastq Paired file: SRR26075334_2.fastq trimmed: SRR26075334-trimmed-pair1.fastq, SRR26075334-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 20:40:41 2025 >> started Tue Feb 11 20:42:42 2025 >> done (121.170s) 79912534 read pairs processed; of these: 1516 ( 0.00%) short read pairs filtered out after trimming by size control 1741449 ( 2.18%) empty read pairs filtered out after trimming by size control 78169569 (97.82%) read pairs available; of these: 10122532 (12.95%) trimmed read pairs available after processing 68047037 (87.05%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 51 0.00% 19 86 0.00% 20 95 0.00% 21 118 0.00% 22 161 0.00% 23 182 0.00% 24 189 0.00% 25 242 0.00% 26 228 0.00% 27 218 0.00% 28 302 0.00% 29 291 0.00% 30 316 0.00% 31 333 0.00% 32 250 0.00% 33 336 0.00% 34 404 0.00% 35 378 0.00% 36 369 0.00% 37 486 0.00% 38 1056 0.00% 39 690 0.00% 40 1152 0.00% 41 644 0.00% 42 703 0.00% 43 633 0.00% 44 669 0.00% 45 699 0.00% 46 862 0.00% 47 913 0.00% 48 922 0.00% 49 965 0.00% 50 1192 0.00% 51 1244 0.00% 52 1730 0.00% 53 1392 0.00% 54 1894 0.00% 55 3109 0.00% 56 1731 0.00% 57 2320 0.00% 58 4639 0.01% 59 2318 0.00% 60 4585 0.01% 61 2396 0.00% 62 2519 0.00% 63 4226 0.01% 64 2719 0.00% 65 2960 0.00% 66 2666 0.00% 67 2809 0.00% 68 3160 0.00% 69 3186 0.00% 70 3570 0.00% 71 3978 0.01% 72 4527 0.01% 73 4721 0.01% 74 4939 0.01% 75 5163 0.01% 76 5636 0.01% 77 6110 0.01% 78 6313 0.01% 79 6607 0.01% 80 7615 0.01% 81 8719 0.01% 82 9481 0.01% 83 10385 0.01% 84 11511 0.01% 85 12546 0.02% 86 13292 0.02% 87 14058 0.02% 88 14931 0.02% 89 16494 0.02% 90 17515 0.02% 91 19416 0.02% 92 21993 0.03% 93 24339 0.03% 94 26712 0.03% 95 28694 0.04% 96 30614 0.04% 97 33096 0.04% 98 35469 0.05% 99 37421 0.05% 100 40114 0.05% 101 44806 0.06% 102 48997 0.06% 103 53979 0.07% 104 58688 0.08% 105 63232 0.08% 106 67655 0.09% 107 72132 0.09% 108 75391 0.10% 109 79498 0.10% 110 84002 0.11% 111 90489 0.12% 112 96589 0.12% 113 103862 0.13% 114 111925 0.14% 115 119469 0.15% 116 125992 0.16% 117 132771 0.17% 118 137094 0.18% 119 140839 0.18% 120 146928 0.19% 121 154481 0.20% 122 162331 0.21% 123 171911 0.22% 124 183240 0.23% 125 190117 0.24% 126 198825 0.25% 127 204206 0.26% 128 209173 0.27% 129 215067 0.28% 130 220190 0.28% 131 225535 0.29% 132 233049 0.30% 133 242676 0.31% 134 252213 0.32% 135 263952 0.34% 136 269852 0.35% 137 272912 0.35% 138 277494 0.35% 139 282441 0.36% 140 285649 0.37% 141 288960 0.37% 142 295519 0.38% 143 301630 0.39% 144 316222 0.40% 145 322405 0.41% 146 328534 0.42% 147 332975 0.43% 148 334437 0.43% 149 336993 0.43% 150 341488 0.44% 151 68047037 87.05% 78169569 reads passed initial QC criterion=sequence-density sequence-density=0.30 sequence-density-rank=1 fanout-score=3.56 fanout-score-rank=27 prefix-density=0.46 prefix-fanout=2.3 sequence=CACTTGCAGCCATTCTCAGCACCAGAG criterion=fanout-score sequence-density=0.05 sequence-density-rank=29 fanout-score=361.85 fanout-score-rank=1 prefix-density=0.83 prefix-fanout=22.0 sequence=TCATCATCACCACCATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTC criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=3.02 fanout-score-rank=27 prefix-density=0.37 prefix-fanout=3.0 sequence=ATGTACCCTGACTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=232.11 fanout-score-rank=1 prefix-density=0.18 prefix-fanout=11.4 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAAAGATCAGCACCAGACCACTTAACATCAAAGATATTGACGTCGGAGAGGGGAGCAGTCCTGTTAATAAGTTTCAGAGGTCCATGACTATGCCAGGAACTCCAGGGACACCGACGACACCAGTGACCCCTACAACCCCAGTGTCGGCGCGTAGCAATGTTTGGAGGAGCGTGTTCCACCCTGGTAGCAACCTTGCTACTAAGAATATTGGTGCTCATGTTTTTGACAAGCCACAGCCTAACACACCCACTGTCTATGACTGGATGTACAGTGGAGAGACGAAGAGCGAGCATCGTTGATGAGGTTGCCTTCAACCAAGGTTGCCCATGTAAATACGTACTGTGTTTTGTTTTTCAGTACTCATCTGCAATATGTCTCTTGTTGTTATGGTTCTACGGTTCTACCGTGCCTGGAA SRR26075334 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 20:43:32 Started mapping on | Feb 11 20:43:32 Finished on | Feb 11 20:59:44 Mapping speed, Million of reads per hour | 289.52 Number of input reads | 78169569 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 67060094 Uniquely mapped reads % | 85.79% Average mapped length | 294.91 Number of splices: Total | 64957409 Number of splices: Annotated (sjdb) | 63366964 Number of splices: GT/AG | 63711534 Number of splices: GC/AG | 962770 Number of splices: AT/AC | 77806 Number of splices: Non-canonical | 205299 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 3.05 Insertion rate per base | 0.02% Insertion average length | 2.50 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1670322 % of reads mapped to multiple loci | 2.14% Number of reads mapped to too many loci | 148224 % of reads mapped to too many loci | 0.19% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 11.07% % of reads unmapped: other | 0.81% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 9439153 9439153 9439153 N_multimapping 1670322 1670322 1670322 N_noFeature 1693713 66310438 2101765 N_ambiguous 714927 3963 371232 UnstrandedReadsAssigned:64651454 PositiveStrandReadsAssigned:745693 NegativeStrandReadsAssigned:64587097 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075334 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075334-trimmed-pair1.fastq SRR26075334-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 78,169,569 reads, 65,906,126 reads pseudoaligned [quant] estimated average fragment length: 217.871 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,280 rounds 52401 SRR26075334.ke.tsv 34699 SRR26075334.se.tsv 87100 total ==> SRR26075334.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1801.13 7506 53.3027 Potri.005G024800.1.v4.1 1035 818.129 4113 64.3017 Potri.004G059700.1.v4.1 961 744.134 8 0.137507 Potri.007G009000.2.v4.1 1416 1199.13 0 0 Potri.003G141000.2.v4.1 2943 2726.13 4298.97 20.1699 Potri.016G087400.1.v4.1 270 86.1952 8183 1214.27 Potri.015G069301.1.v4.1 564 349.35 0 0 Potri.010G195200.1.v4.1 1773 1556.13 2147.81 17.6537 Potri.012G127500.1.v4.1 977 760.134 18478 310.921 ==> SRR26075334.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 486 Potri.001G233950.v4.1 1 Potri.001G122700.v4.1 2007 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1884 SRR26075334 completed mapping pipeline successfully