Starting /dee2/code/volunteer_pipeline.sh SRR26075335 current disk space = 3052898291712 free memory = 1474541968 SRR26075335 SRAfilesize 4acf3a500b8f6441e59f8dc3ea2a9824 SRR26075335.sra SRR26075335.sra file validated SRR26075335 is paired end SRR26075335 is conventional basespace SRR26075335 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075335_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.512 37.0 37.0 37.0 37.0 37.0 2 36.558 37.0 37.0 37.0 37.0 37.0 3 36.5985 37.0 37.0 37.0 37.0 37.0 4 36.636 37.0 37.0 37.0 37.0 37.0 5 36.6865 37.0 37.0 37.0 37.0 37.0 6 36.621 37.0 37.0 37.0 37.0 37.0 7 36.741 37.0 37.0 37.0 37.0 37.0 8 36.6795 37.0 37.0 37.0 37.0 37.0 9 36.653 37.0 37.0 37.0 37.0 37.0 10-14 36.637 37.0 37.0 37.0 37.0 37.0 15-19 36.6207 37.0 37.0 37.0 37.0 37.0 20-24 36.5338 37.0 37.0 37.0 37.0 37.0 25-29 36.4489 37.0 37.0 37.0 37.0 37.0 30-34 36.40840000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.3334 37.0 37.0 37.0 37.0 37.0 40-44 36.2765 37.0 37.0 37.0 37.0 37.0 45-49 36.205299999999994 37.0 37.0 37.0 37.0 37.0 50-54 36.1417 37.0 37.0 37.0 37.0 37.0 55-59 36.123599999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.062599999999996 37.0 37.0 37.0 37.0 37.0 65-69 35.985499999999995 37.0 37.0 37.0 37.0 37.0 70-74 35.9715 37.0 37.0 37.0 37.0 37.0 75-79 36.0163 37.0 37.0 37.0 37.0 37.0 80-84 36.022499999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.9228 37.0 37.0 37.0 37.0 37.0 90-94 35.889700000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.873000000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.8115 37.0 37.0 37.0 37.0 37.0 105-109 35.8544 37.0 37.0 37.0 37.0 37.0 110-114 35.6581 37.0 37.0 37.0 37.0 37.0 115-119 35.6097 37.0 37.0 37.0 37.0 37.0 120-124 35.652 37.0 37.0 37.0 37.0 37.0 125-129 35.4947 37.0 37.0 37.0 37.0 37.0 130-134 35.3845 37.0 37.0 37.0 34.6 37.0 135-139 35.3258 37.0 37.0 37.0 34.6 37.0 140-144 35.2305 37.0 37.0 37.0 29.8 37.0 145-149 35.2697 37.0 37.0 37.0 32.2 37.0 150-151 35.244 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 3.0 20 1.0 21 0.0 22 2.0 23 6.0 24 15.0 25 9.0 26 6.0 27 15.0 28 10.0 29 19.0 30 28.0 31 49.0 32 68.0 33 86.0 34 139.0 35 434.0 36 2946.0 37 164.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.02558956347215 13.49724034119418 8.078273958855995 35.39889613647767 2 19.950000000000003 13.025 35.875 31.15 3 18.099999999999998 17.625 31.0 33.275 4 21.075 25.674999999999997 26.55 26.700000000000003 5 22.400000000000002 30.225 25.85 21.525 6 22.1 35.025 21.875 21.0 7 14.75 27.250000000000004 39.525 18.475 8 16.950000000000003 26.924999999999997 32.35 23.775 9 19.075 22.3 35.725 22.900000000000002 10-14 19.906990699069908 28.852885288528853 28.28282828282828 22.95729572957296 15-19 20.16 27.500000000000004 27.855 24.485 20-24 20.145 27.52 28.365000000000002 23.97 25-29 19.805 28.1 27.785 24.310000000000002 30-34 19.8 28.32 28.215 23.665 35-39 20.36 28.485 27.229999999999997 23.925 40-44 20.325 28.725 26.779999999999998 24.169999999999998 45-49 20.455000000000002 27.875 28.16 23.51 50-54 21.029999999999998 28.025 27.750000000000004 23.195 55-59 20.665 28.48 26.97 23.885 60-64 21.01 28.65 27.339999999999996 23.0 65-69 20.84 27.155 27.865000000000002 24.14 70-74 21.029999999999998 28.405 27.005000000000003 23.56 75-79 21.63 28.1 27.310000000000002 22.96 80-84 20.89 27.02 27.455000000000002 24.635 85-89 21.325 27.96 26.784999999999997 23.93 90-94 20.73 27.42 27.894999999999996 23.955000000000002 95-99 21.240000000000002 27.560000000000002 27.779999999999998 23.419999999999998 100-104 21.435000000000002 28.49 27.145000000000003 22.93 105-109 21.6 27.834999999999997 27.775 22.79 110-114 21.465 27.224999999999998 27.615000000000002 23.695 115-119 21.845 27.49 27.21 23.455000000000002 120-124 21.279999999999998 28.175 27.47 23.075000000000003 125-129 20.990000000000002 27.49 27.889999999999997 23.630000000000003 130-134 20.995 27.455000000000002 28.065 23.485 135-139 20.945 28.32 27.18 23.555 140-144 21.475 27.450000000000003 26.655 24.42 145-149 22.465 28.405 26.314999999999998 22.814999999999998 150-151 21.4 27.037499999999998 26.8 24.762500000000003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 1.0 11 1.5 12 0.5 13 0.0 14 0.0 15 0.0 16 1.0 17 1.0 18 0.5 19 1.0 20 2.0 21 2.0 22 3.0 23 3.5 24 1.5 25 3.5 26 4.0 27 5.0 28 10.5 29 10.5 30 12.5 31 20.0 32 18.0 33 27.0 34 39.0 35 51.5 36 73.5 37 84.0 38 116.5 39 177.0 40 194.0 41 203.5 42 248.5 43 268.0 44 281.0 45 298.0 46 273.5 47 237.0 48 229.5 49 208.0 50 162.0 51 128.0 52 106.0 53 97.0 54 82.0 55 61.0 56 51.5 57 43.0 58 33.0 59 25.0 60 17.0 61 10.0 62 13.5 63 13.5 64 9.0 65 6.5 66 3.0 67 1.5 68 3.0 69 2.5 70 1.0 71 3.0 72 3.0 73 2.5 74 2.5 75 2.0 76 2.0 77 1.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.6 #Duplication Level Percentage of deduplicated Percentage of total 1 65.41533546325878 40.949999999999996 2 20.64696485623003 25.85 3 7.867412140575079 14.774999999999999 4 3.2747603833865817 8.200000000000001 5 1.5575079872204471 4.875 6 0.7188498402555911 2.7 7 0.1996805111821086 0.8750000000000001 8 0.1597444089456869 0.8 9 0.07987220447284345 0.44999999999999996 >10 0.07987220447284345 0.525 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTGGGTTCATCATCCACTCCTTCAACATCATAATCACTGGAATCGCTGA 11 0.27499999999999997 No Hit GTATCTTCTTTATGAGAGTGGAATTTCTGATCAGCCCATCAAAGACCAAG 10 0.25 No Hit ACCAGCAGCACTGCGCGAAAAGATTCCCATTATATTTACAATTTCTGGAC 9 0.22499999999999998 No Hit CAGAAATTTGAATGATGCGTCGCCAGCACGAAGGCCGTGCGATCCGTCGA 9 0.22499999999999998 No Hit CCTCGCTGTAAGATTTGATGATGAAAAATTTAGCATTATCATATTCCTCA 8 0.2 No Hit GGACATTTGTTGTTTCTTCCTCTTCTGGAAGTTCTTTGAAGTACCAACAA 8 0.2 No Hit TGCAAATCTTCTTTCCTTGTCATAAAATCACCAACTGTATATACCCCACT 8 0.2 No Hit CTTGTCAAGAGCATGGTCTTCGGAGTAACTTTCATCAGAATGTGGTTGAG 8 0.2 No Hit AATAAATAAGACAAGGCGGAATTGTTTATACGTCCCATCTCGAATACTTC 7 0.17500000000000002 No Hit AGCTTGCTCTTCTTCGGCACGAAGGTCACGGTAACACTTGGAGCAAAGGT 7 0.17500000000000002 No Hit GCCACAACAATATTCTGAAACAGCATGCTAGTTCAAAATGCAAATTAAAT 7 0.17500000000000002 No Hit GCACTGTTAGATATTGAAGGCCATGGGTCACTTTCAAAGTCAATGTCTCC 7 0.17500000000000002 No Hit AGCTAATGCTGCAGCTCGATCCCATTCTAGTACTGCACCAAATATAGATC 7 0.17500000000000002 No Hit CCTGAATTTTGCTCCTGCATATACTGCTGCAGATCCCAGCTCTTCCTCTA 6 0.15 No Hit CTCTCCAATTGCATATCAACTCCATGCAGTTCATTGCTTAAATCCTGAGC 6 0.15 No Hit GCCATGCCACGGGACTTATATCCCTTAGCTGAATCCAGGTTAATCTCAAG 6 0.15 No Hit CCCCATTTCCCAGATCCAAATGTTTCAATGCCCAATTAATTAAAATTTCA 6 0.15 No Hit CTGAGAAAGGAGTCGAGCTCTACGCAACATTCAACACATCCGGCAAGGCG 6 0.15 No Hit CTCATGTGCAAAAACAAAATGTCACCCCTCTTGAAGCCAGGTTCCATACT 6 0.15 No Hit GTCGCAAAAAACCAGCAAAAATTCTTAACATTATTTTTAGAAATCCCATA 6 0.15 No Hit GTATCAGAGAAGGGAATAGCAAGCCAAGGCATTTCGGAGAAGTATGTGTT 6 0.15 No Hit CCGGCAGCAAGAGAGTGTACAGACACGTCCTGGAGCTGATCTTGTTCGTC 6 0.15 No Hit CCAGCTAATAGATCTATCACAGGCCCTGTACCAAGGGCAGGGCAATTATT 6 0.15 No Hit CTTGGGTGCGCTTTCGCCTTTACCGTGCCTTTAAACGGTGCACCTGTACC 6 0.15 No Hit TCCTGGATGGTTCCATTTGTAGCTAAAGCGGTGGGCATTGCTGGAATTTC 6 0.15 No Hit GTTGAACTACTAGTTCCCCTTCTATGGCTACATGTTTCTCAGCGACCTCG 6 0.15 No Hit GGCCATCACAGTTGTAAGTAAACTTGTTGTTAGTAGCAGGAAGTTTCTGG 6 0.15 No Hit CTCTCGGCAATCATAAGATATGGAGTTTTCATACTGCAGAGATGTCCTAA 6 0.15 No Hit AGCTCTTCCTCTACTTTAGTCTCGGGTTCAGAAACCTGAAGTTTCTCTTC 6 0.15 No Hit AAAGGATCATTAACAGCAACAAGTTCAACATCATCTCTCTGTAGAGCTAC 6 0.15 No Hit GTGGCAACTCCGGCAAATTATAAAGCAACTGCACCCCTTTCCTCACTTGA 6 0.15 No Hit CTTGTTTGTTTCTGAACTGGGGTGATTTTCATCACCTCATCTTTCAAGAT 5 0.125 No Hit GTGCTGAGATAAAGTGGCCACTTCTTTTGGTAAGCAGTGTTCATAGAAGC 5 0.125 No Hit GCGCTTGCTCTGAATCCTCAAAATGGCTAGAAGAACTCAGAAGCTCATTC 5 0.125 No Hit GGGTTTTCATCATTGCAGGCTTCTAGTAAGAAAGGGACATAAGTACCATA 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGACACAATCTCGGTT 5 0.125 TruSeq Adapter, Index 7 (97% over 37bp) CATCATTCACAAGCTATTTTGGTGTCAAAGCTACTGAGGCACAGTGCAAA 5 0.125 No Hit GTTTTAAGCTCATTGATATACGATATTGCATCGCCAAGAAGGGAAGCTTT 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGACACAATCTCGGGT 5 0.125 TruSeq Adapter, Index 7 (97% over 37bp) GTCATGTAAAAATTTAGAGTCTCGAGAACTGTATTGTTCGTAGCAAGCTC 5 0.125 No Hit CATTTATTTTTATTTTTATTTTATTGCAAGTTCAATTACTCGATTAGTGG 5 0.125 No Hit CTCAGTCAACCTTGAAATCAGAGACCACGTCTCAAAAGATTTCTCTTTAG 5 0.125 No Hit CAGTCCTCAATATGCCTCGGCAAACCCAAGCTCTGAGAGCTTCATATGAG 5 0.125 No Hit CGAGCAATGGATGTCTTACCAGTTCCAGGTGGGCCCCAGAGGATGATAGA 5 0.125 No Hit GGGCATTCAAGCAGTTCAAACAGAACCCATATAGCAGTAGCACCACCAAG 5 0.125 No Hit CCTCCACATTTGAATTTAGTCACCTGAGCCGTTACAGGAGGCATCTGAAG 5 0.125 No Hit CCTCAAATGTCAACACAGCATTGGGCATGCAGCTGTGGTTGATAATGGAA 5 0.125 No Hit CACCAAAACAACCGAAGAAAGAAAAACCTTAACAGAGAAAGAGAGAAAGT 5 0.125 No Hit CACGTCCTGAAGCGGGAGACGGAGGGGCTTGTCTGAGGGCCTCTTGGGCT 5 0.125 No Hit CATCACTGTTTTGCTCTTCATCTTGTACCAAATCTAGGCCTTGCAGTTTG 5 0.125 No Hit CACTTGGACATCTTCTCCAAGGATTGTCATGTTCTCTACACGGGCCCATC 5 0.125 No Hit CTCGTGTCATTCACAGTTTTGTTGCTATAAATTTGCCCCACTACAACACT 5 0.125 No Hit ATCCCATTGTTGCAAACCCAAACCTTGAAGTCCCCACCATAAGAAGAACT 5 0.125 No Hit ACCAGTTCCCTGAATTGCAGGATGCATATGGCTATGAAAACCATTAGAAA 5 0.125 No Hit CTCTGCCACAGTGTAAATCCCCTTATCATTTGTGAAGGCCTGGTAGTTTA 5 0.125 No Hit GGGGGATACGAGAAACGGCACGACCAGTACCCCATGATTCGGCTGAGGTC 5 0.125 No Hit GTTGGCGACCTGCTGGGAGCGAAGAAGAAGACGATCCTTGTAGTCGGCCC 5 0.125 No Hit TCTTCTTCTTCTTGTCTCCAGCTACTGCTGCTCCGCCTTCTTTTGGGAGC 5 0.125 No Hit TTCCCAACTCCTTGAAGAGCACCTATGGGCTGCCAAAGAGCAGAAAAAGT 5 0.125 No Hit GTGCGTTTCTGGCCTTCAAGAAACACTTCTCTCTTGATGATAAACCCTTC 5 0.125 No Hit GCATCGTCTGAATTTGCTGGTGCTCCAACTTAACTGGACCCATATTTCTC 5 0.125 No Hit ATCCAGATCAAAGTTGTTTCATCATTAGAAAGTCTAAACGGACAAAACTT 5 0.125 No Hit CAGTGAGTCATAAACTCATTTTTAATCTTCCGCATGGCCTCATGCTCCCC 5 0.125 No Hit CGGTGATTGACTTTCTCCCAAGCATAACCATAGGCGTAACAAACTGCAAT 5 0.125 No Hit TTTTTTTTTTTCAAGCACGCAGGCAAAAAAGAAGGTTACTTCACATCTTA 5 0.125 No Hit GTGCATCTGAACCCTTTGGACCTAACGATTTCGCATGCTCTGATACACCA 5 0.125 No Hit AGCAGCTCATCTTCTGGTACATAAATCTTCTCAAGCTTCTTCCCAATCTT 5 0.125 No Hit ATTTTGGCTGGTCCACCTTGTAGTCTATAGAATCATTCGTAGCTAGAATT 5 0.125 No Hit GCGGACTTTTATGTTCTCCCCAAGAGCAGCAACAGTCTGTTTCACCAAAT 5 0.125 No Hit CTCGAACTGGCACATTCTCAAGTATTCTTCGAAGCTTCACCTCTAGGTCC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.0625 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.125 0.0 0.0 0.0 0.0 90-91 0.1375 0.0 0.0 0.0 0.0 92-93 0.15 0.0 0.0 0.0 0.0 94-95 0.16249999999999998 0.0 0.0 0.0 0.0 96-97 0.21250000000000002 0.0 0.0 0.0 0.0 98-99 0.2625 0.0 0.0 0.0 0.0 100-101 0.3375 0.0 0.0 0.0 0.0 102-103 0.3875 0.0 0.0 0.0 0.0 104-105 0.4375 0.0 0.0 0.0 0.0 106-107 0.4875 0.0 0.0 0.0 0.0 108-109 0.575 0.0 0.0 0.0 0.0 110-111 0.7875000000000001 0.0 0.0 0.0 0.0 112-113 1.0625 0.0 0.0 0.0 0.0 114-115 1.2625000000000002 0.0 0.0 0.0 0.0 116-117 1.4 0.0 0.0 0.0 0.0 118-119 1.5750000000000002 0.0 0.0 0.0 0.0 120-121 1.65 0.0 0.0 0.0 0.0 122-123 1.925 0.0 0.0 0.0 0.0 124-125 2.325 0.0 0.0 0.0 0.0 126-127 2.875 0.0 0.0 0.0 0.0 128-129 3.2625 0.0 0.0 0.0 0.0 130-131 3.9125 0.0 0.0 0.0 0.0 132-133 4.375 0.0 0.0 0.0 0.0 134-135 4.800000000000001 0.0 0.0 0.0 0.0 136-137 5.262499999999999 0.0 0.0 0.0 0.0 138-139 6.0875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGATT 10 0.006832588 144.9875 9 TCGCTGT 10 0.006832588 144.9875 3 GTAAGAT 10 0.006832588 144.9875 8 CTGTAAG 15 1.14152615E-4 144.9875 6 CGCTGTA 10 0.006832588 144.9875 4 >>END_MODULE SRR26075335 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075335_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0435 37.0 37.0 37.0 37.0 37.0 2 36.0195 37.0 37.0 37.0 37.0 37.0 3 36.0895 37.0 37.0 37.0 37.0 37.0 4 36.044 37.0 37.0 37.0 37.0 37.0 5 36.104 37.0 37.0 37.0 37.0 37.0 6 35.9895 37.0 37.0 37.0 37.0 37.0 7 36.0605 37.0 37.0 37.0 37.0 37.0 8 36.08 37.0 37.0 37.0 37.0 37.0 9 36.252 37.0 37.0 37.0 37.0 37.0 10-14 35.984300000000005 37.0 37.0 37.0 37.0 37.0 15-19 35.897299999999994 37.0 37.0 37.0 37.0 37.0 20-24 35.8915 37.0 37.0 37.0 37.0 37.0 25-29 35.7687 37.0 37.0 37.0 37.0 37.0 30-34 35.6214 37.0 37.0 37.0 37.0 37.0 35-39 35.6185 37.0 37.0 37.0 37.0 37.0 40-44 35.5619 37.0 37.0 37.0 37.0 37.0 45-49 35.464 37.0 37.0 37.0 37.0 37.0 50-54 35.3224 37.0 37.0 37.0 37.0 37.0 55-59 35.350300000000004 37.0 37.0 37.0 37.0 37.0 60-64 35.384100000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.3002 37.0 37.0 37.0 34.6 37.0 70-74 35.2457 37.0 37.0 37.0 34.6 37.0 75-79 35.1691 37.0 37.0 37.0 34.6 37.0 80-84 35.2638 37.0 37.0 37.0 34.6 37.0 85-89 35.2139 37.0 37.0 37.0 37.0 37.0 90-94 35.1007 37.0 37.0 37.0 32.2 37.0 95-99 35.1495 37.0 37.0 37.0 29.8 37.0 100-104 35.006600000000006 37.0 37.0 37.0 27.4 37.0 105-109 34.981700000000004 37.0 37.0 37.0 27.4 37.0 110-114 34.8817 37.0 37.0 37.0 27.4 37.0 115-119 34.9209 37.0 37.0 37.0 25.0 37.0 120-124 34.8603 37.0 37.0 37.0 25.0 37.0 125-129 34.8174 37.0 37.0 37.0 25.0 37.0 130-134 34.826299999999996 37.0 37.0 37.0 25.0 37.0 135-139 34.6715 37.0 37.0 37.0 25.0 37.0 140-144 34.6822 37.0 37.0 37.0 25.0 37.0 145-149 34.6087 37.0 37.0 37.0 25.0 37.0 150-151 34.251 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 2.0 13 5.0 14 9.0 15 12.0 16 12.0 17 10.0 18 9.0 19 8.0 20 3.0 21 9.0 22 11.0 23 11.0 24 18.0 25 19.0 26 22.0 27 18.0 28 16.0 29 21.0 30 34.0 31 39.0 32 68.0 33 103.0 34 255.0 35 740.0 36 2393.0 37 152.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.95 23.175 10.125 21.75 2 28.375 24.55 27.35 19.725 3 23.95 28.549999999999997 28.15 19.35 4 27.675 32.800000000000004 21.9 17.625 5 26.275 35.099999999999994 21.55 17.075000000000003 6 24.175 38.775 20.674999999999997 16.375 7 22.925 23.549999999999997 35.35 18.175 8 25.174999999999997 24.55 25.724999999999998 24.55 9 23.849999999999998 25.474999999999998 26.900000000000002 23.775 10-14 25.480000000000004 28.470000000000002 24.98 21.07 15-19 25.185000000000002 27.58 27.229999999999997 20.005 20-24 25.424999999999997 28.494999999999997 26.185000000000002 19.895 25-29 25.374999999999996 28.084999999999997 25.979999999999997 20.560000000000002 30-34 24.83 28.17 26.905 20.095 35-39 24.87 27.595 26.939999999999998 20.595 40-44 25.055 28.405 26.515 20.025000000000002 45-49 25.19 28.425 26.740000000000002 19.645000000000003 50-54 23.49 28.595 27.095000000000002 20.82 55-59 25.005 28.335 26.25 20.41 60-64 24.485 28.244999999999997 26.705000000000002 20.565 65-69 25.155 28.21 26.51 20.125 70-74 23.96 29.67 26.165 20.205000000000002 75-79 23.82 29.604999999999997 26.095000000000002 20.48 80-84 24.565 28.49 26.25 20.695 85-89 25.775 28.634999999999998 25.729999999999997 19.86 90-94 24.725 29.085 26.445 19.744999999999997 95-99 24.875 28.449999999999996 26.085 20.59 100-104 25.835 27.845 26.325 19.994999999999997 105-109 25.264999999999997 27.884999999999998 26.87 19.98 110-114 24.485 28.21 26.615 20.69 115-119 24.975 28.7 27.025 19.3 120-124 24.97 27.650000000000002 27.33 20.05 125-129 24.16 28.249999999999996 27.54 20.05 130-134 24.965 28.22 27.045 19.77 135-139 25.580000000000002 28.87 25.75 19.8 140-144 25.35 27.529999999999998 27.075 20.044999999999998 145-149 26.19 29.189999999999998 25.365 19.255 150-151 26.974999999999998 28.6875 24.825 19.5125 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 3.0 9 3.5 10 2.5 11 1.5 12 3.5 13 4.0 14 3.0 15 3.0 16 1.5 17 1.0 18 0.5 19 4.0 20 5.0 21 1.0 22 0.5 23 3.0 24 2.5 25 1.0 26 5.0 27 8.5 28 6.0 29 5.5 30 8.5 31 8.5 32 20.5 33 33.0 34 46.0 35 49.5 36 61.5 37 85.0 38 121.0 39 163.0 40 194.5 41 242.5 42 257.0 43 266.0 44 258.5 45 245.5 46 248.0 47 242.0 48 241.5 49 218.5 50 170.5 51 131.5 52 104.0 53 86.0 54 72.5 55 51.5 56 43.5 57 37.5 58 26.5 59 16.5 60 14.0 61 16.5 62 13.5 63 8.0 64 7.0 65 11.0 66 9.0 67 2.5 68 4.0 69 5.0 70 3.5 71 2.0 72 2.0 73 2.5 74 2.0 75 2.0 76 1.5 77 1.5 78 2.0 79 1.5 80 1.0 81 2.5 82 3.5 83 1.5 84 0.5 85 4.0 86 5.5 87 2.5 88 1.0 89 2.5 90 2.5 91 0.5 92 1.5 93 3.0 94 3.0 95 1.5 96 1.0 97 1.5 98 0.5 99 1.0 100 14.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.1 #Duplication Level Percentage of deduplicated Percentage of total 1 67.0364500792393 42.3 2 19.69096671949287 24.85 3 7.6069730586370845 14.399999999999999 4 2.8922345483359746 7.3 5 1.5055467511885896 4.75 6 0.7923930269413629 3.0 7 0.19809825673534073 0.8750000000000001 8 0.07923930269413629 0.4 9 0.039619651347068144 0.22499999999999998 >10 0.15847860538827258 1.9 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 42 1.05 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 14 0.35000000000000003 No Hit GCAAGCTTGTTCGCCAGTCTAAATGTTTGCAGGATAAGATGACTGCCAAG 10 0.25 No Hit GCAGAGATTTGAGTCTGACAGAGGTCTTTCCTCTTAAGTAGTGAAGAGAC 10 0.25 No Hit CTGGAAGGGACGCATTTATTAGATAAAAGGTCGACGCGGGCTCTGCCCGT 9 0.22499999999999998 No Hit CTACACTCCGACTCTCTCTCCCGCACACGCCTGTGCGTCACCACCACCAC 8 0.2 No Hit TTCTTCATTTCAATTCTCAGAGGAACTCGGGATTTCTTCTACAATTTGCA 8 0.2 No Hit GAAGATGTGAGGCCTCTAATTGCAAAGAAATACAATCTTCGGCAGAAAAT 7 0.17500000000000002 No Hit GTCGTTTCCAGACAACACAGGAGAAACAGAAGTTCTATGGACGCCTGAAG 7 0.17500000000000002 No Hit GCGAACAGTATTCTCCTGCTTCATTCAAGCAGTTTACTTGCTTGAAACTG 7 0.17500000000000002 No Hit GTGTAACCAACACCTGCGAGGTCTGCGAGCGCAGCCTCCTCGATTCCTTC 7 0.17500000000000002 No Hit TATGTTGCTCCTGAAGTATTGCGCCGTAGTTATGGAAAGGAAATAGACAT 7 0.17500000000000002 No Hit CCGTACTGCTGCCCGTGAAGCAATAGCAAAAGCCAATCCTGTTGTCAAGG 6 0.15 No Hit TATTCGGAGTCGAAACTCTTGCTGGTGTTCTTGCTGGTTCACTTGTTTCT 6 0.15 No Hit CAATTCTCTCCTCCTCTTTCATAGCCTCACTCTTTCAACTTCCAGAAAGA 6 0.15 No Hit CTTGAAAGAACTAAAATACTCTTACAGACAAGAACTGAAGGGTTCCAATC 6 0.15 No Hit ATCTGGAGGATCAGCTGTTAGGGTTTGCTCTGGTTCCGTTGTCTGAAGTT 6 0.15 No Hit GTGATATCCCTGATCCTCCTGGCTTCTATCGCACCTCTCAAGAGCAGGAT 6 0.15 No Hit CCAGTAATGGACGAGATCCGGGAAAATGGGGACAGGGTCAACATTTGGCA 6 0.15 No Hit TGCATGGGCACATTGATCAAAACCTTCAAGGCCTCTTTCCTGCCTTTCTT 6 0.15 No Hit CTTTTACTCTCTCTCTCTCTCTCTCCTTCCCAAAAGCAACAAGGAAACAA 6 0.15 No Hit GACCAAGTCAGATTACATAACTTTACGGCTAGGATTTATCATGACACATA 6 0.15 No Hit AGAGAAGAAAAGGAAAATGGGTTGGATCGGAGACACCGTAGACTCCGTTA 6 0.15 No Hit AGACTTGTTTTTAAGAATGCAATGAAATATAATGATGAAAGAGATGATGT 6 0.15 No Hit GCTTTTACTCCTACTGTTTATCTCTGTGAATCATGGGAAAGATCAAGATT 6 0.15 No Hit ATTTCAAAAACCAAAAGGGTTTTGTTCCTGCCACAGTTGCAGTCAAGGGG 6 0.15 No Hit TCTAGCTAGAAAGTATCAAAATGGTATTTTATCGGATTCTGCGATTGTAA 6 0.15 No Hit GTTACAAGCGCAAATCACTCGAAGCAGAAGCTTACTCATTTTAATTACTC 6 0.15 No Hit GTGCGGCCCATGCCGTAATTTCACTCCATTGTTGGTAGAAGTCTATGAAC 6 0.15 No Hit GCAATAATGTATGCAACAAGAGCTACTGTTTACATCAAAATGAAGAGGCC 6 0.15 No Hit AGCAACAAAATCAACACCACCGTCGATTCGATATGGCTCCGATTGCTGTC 6 0.15 No Hit CGCATTTCAGGCTCTCAAGTGTACTGTACAATCTCTCGCTCTGTCTCTGA 6 0.15 No Hit AGCAAAGGCAATGGGACACCATGTAACTGTGATTAGTTCTTCTGACAAGA 5 0.125 No Hit GATGAGAAGAGGAATAGACTATTTGTGGTTGCATGTCGTGTCTTGAATTT 5 0.125 No Hit GAATGAAAATAATGTGCTTGAATTGCTAAAATTTCTGCAACTGTGAGGTC 5 0.125 No Hit GGAGCTTTGGTGCTGAAAAACAAGGCTCCAAGGTGGCATGAACAGCTCCA 5 0.125 No Hit CCAAGCTGGAGGTCAAGGATATTGCTGATATAATGTTGAAGGAGGTCTTT 5 0.125 No Hit CGCACATCGGTCGCTCTTGCAGGCAATTGAGAACATTATTCTTGGAAGAG 5 0.125 No Hit GGTATGATGAAATTGTCAAGGAAGTGTCCTCCTACTTGAAGAAGGTTGGT 5 0.125 No Hit TTAAACGCGTTTCACATAATATCTTTATCCCAGGGATTTGATTTGTCACC 5 0.125 No Hit TAACGAACTTGATGGTTGAAGTGTTGGAAACGGTAGACCAACTTGCTTCC 5 0.125 No Hit AAGGAACAGGATGTTGCTGCCTTCACAATATGTTCAATGGATGACCCACG 5 0.125 No Hit CTGAGAACATTCGGAACAAAGAAAAGGAGCTTGAAATGCAGAGAGATGAT 5 0.125 No Hit CCGTAACTCCATCTACGTTACCTTCGTCATCGCCGGCGCTTTTGCCGGTG 5 0.125 No Hit GGGTAGAAAAGTGTATTCGAAATAAGTTTCCAAGCGGGAAGACATGCAAT 5 0.125 No Hit GAAGGGCCTGTACTTTCTCTCAAACCTTGATCAAAATATCGCAGTCATAG 5 0.125 No Hit GCCATGGATTCACAACTGAGAAAGCTGGTAGCGGGTCTTTTTCTACTGTC 5 0.125 No Hit TATGAAGATGGCGAGATGCAGCTCTGGGAAGTCACCGGATCACCTATGAT 5 0.125 No Hit AAACCATCAACCAGGCGATGAAGTCACGGTAGTAGTGACATTAGCTGTTC 5 0.125 No Hit GGAATTTCCGAGGGCTCATCATGTCTAGTGAGAGAAAATAAGGAAACATC 5 0.125 No Hit GGACTGGCAGCGGGGCCTTTCGATAAAGTTATGGTGTGGACCGAGGTCTT 5 0.125 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 5 0.125 No Hit AGCAACTGGTGCTATATGCACAGATGGCCAACCTTGTCCACTTCATTCTT 5 0.125 No Hit GTTTCACTCTTTTCTGTTCTAGGAAAGACAAGTTTCTATCTTTTAAGTCA 5 0.125 No Hit TGAGAATCACCATGTCAGGAGTCCCGTTGTGGTGAAGAAGATGGCAGTAG 5 0.125 No Hit AGATGGCAGAGCATGATAAGGAGGATTCAGTGATCGAGTCTGTGATGGAG 5 0.125 No Hit GCCAGAAGGACAGGATGAGAAGACAGAGTTGGAGGTGTACAACTTTACAG 5 0.125 No Hit CGAGGGGTATTGATGTGAAGAGGAAGTAGATGGTATATGATGAACGGAGC 5 0.125 No Hit GAGGAGTTCAAATTGAGGGTTGTATATATCCCTGCAAATCCTCCCTCTCC 5 0.125 No Hit GAAACCCTTCTGGAGGATCCAGTCTTCCGCCCCCTTGTTGAAAAATATGC 5 0.125 No Hit GGGGCGAGTATTCCCAATGTATGGTCAGAATATCCCACAGGGATATTGAA 5 0.125 No Hit GCCGCACGCCCCCTGGTCTCCGTACAAACCCTCCCCTGCCTCAACGACAT 5 0.125 No Hit CCAGGGTTCTGTTTCTTTCATCATTCTCTTTCGTTTTTTTCTTTTAATTC 5 0.125 No Hit CGGGGAGGCCGAGGTGGACGTGGAGATCGCGGTGGACGAGGCCGACGCCG 5 0.125 No Hit CAAAAAACTCTCTTAGCCTCTCCTCCTCTGGTTTCGTCGCCTTCGAGAAA 5 0.125 No Hit GTTGAAGCAGAGAGGCAAAGAAGAGAGAAACTTAATCAGAGGTTTTATGC 5 0.125 No Hit ATTGGAGAGGGTTGTTTGATTGGACCTGATGTTGCAATAGGACCAGGATG 5 0.125 No Hit GCCAGAGCCCCGTCACCAGCCCGGCCACCCACCGCCAGAGCCCCATCCAC 5 0.125 No Hit AGCAGTGAATTAGGGTTTTGGATCAGAATGTCGACGGGCGGGGCCAAGGA 5 0.125 No Hit GCACCAGTTTCAATCAATACGAGGAGGGTTGGCTGGTGTTGGACCTGTTA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.0625 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.125 0.0 0.0 0.0 0.0 90-91 0.1375 0.0 0.0 0.0 0.0 92-93 0.15 0.0 0.0 0.0 0.0 94-95 0.16249999999999998 0.0 0.0 0.0 0.0 96-97 0.21250000000000002 0.0 0.0 0.0 0.0 98-99 0.275 0.0 0.0 0.0 0.0 100-101 0.4 0.0 0.0 0.0 0.0 102-103 0.4625 0.0 0.0 0.0 0.0 104-105 0.5 0.0 0.0 0.0 0.0 106-107 0.5375000000000001 0.0 0.0 0.0 0.0 108-109 0.725 0.0 0.0 0.0 0.0 110-111 0.9375 0.0 0.0 0.0 0.0 112-113 1.2125 0.0 0.0 0.0 0.0 114-115 1.4375 0.0 0.0 0.0 0.0 116-117 1.575 0.0 0.0 0.0 0.0 118-119 1.75 0.0 0.0 0.0 0.0 120-121 1.8625 0.0 0.0 0.0 0.0 122-123 2.15 0.0 0.0 0.0 0.0 124-125 2.525 0.0 0.0 0.0 0.0 126-127 3.0999999999999996 0.0 0.0 0.0 0.0 128-129 3.4875 0.0 0.0 0.0 0.0 130-131 4.125 0.0 0.0 0.0 0.0 132-133 4.675000000000001 0.0 0.0 0.0 0.0 134-135 5.0625 0.0 0.0 0.0 0.0 136-137 5.55 0.0 0.0 0.0 0.0 138-139 6.525 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAATTT 10 0.006830828 145.0 1 >>END_MODULE Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074259 spots for SRR26075335.sra Written 3074259 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra Read 3074249 spots for SRR26075335.sra Written 3074249 spots for SRR26075335.sra SRR ids: ['SRR26075335.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_rusxshlr SRR26075335.sra spots: 61484990 blocks: [[1, 3074249], [3074250, 6148498], [6148499, 9222747], [9222748, 12296996], [12296997, 15371245], [15371246, 18445494], [18445495, 21519743], [21519744, 24593992], [24593993, 27668241], [27668242, 30742490], [30742491, 33816739], [33816740, 36890988], [36890989, 39965237], [39965238, 43039486], [43039487, 46113735], [46113736, 49187984], [49187985, 52262233], [52262234, 55336482], [55336483, 58410731], [58410732, 61484990]] SRR26075335 file size 22713707 SRR26075335 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075335 SRR26075335_1.fastq SRR26075335_2.fastq Input file: SRR26075335_1.fastq Paired file: SRR26075335_2.fastq trimmed: SRR26075335-trimmed-pair1.fastq, SRR26075335-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 20:50:56 2025 >> started Tue Feb 11 20:52:22 2025 >> done (85.993s) 61484990 read pairs processed; of these: 278 ( 0.00%) short read pairs filtered out after trimming by size control 328603 ( 0.53%) empty read pairs filtered out after trimming by size control 61156109 (99.47%) read pairs available; of these: 6520891 (10.66%) trimmed read pairs available after processing 54635218 (89.34%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 19 0.00% 19 28 0.00% 20 29 0.00% 21 32 0.00% 22 43 0.00% 23 47 0.00% 24 45 0.00% 25 58 0.00% 26 55 0.00% 27 76 0.00% 28 80 0.00% 29 82 0.00% 30 71 0.00% 31 94 0.00% 32 102 0.00% 33 104 0.00% 34 99 0.00% 35 85 0.00% 36 88 0.00% 37 88 0.00% 38 103 0.00% 39 102 0.00% 40 133 0.00% 41 134 0.00% 42 122 0.00% 43 131 0.00% 44 144 0.00% 45 147 0.00% 46 174 0.00% 47 171 0.00% 48 185 0.00% 49 213 0.00% 50 224 0.00% 51 288 0.00% 52 256 0.00% 53 247 0.00% 54 277 0.00% 55 297 0.00% 56 312 0.00% 57 333 0.00% 58 374 0.00% 59 439 0.00% 60 445 0.00% 61 499 0.00% 62 528 0.00% 63 575 0.00% 64 662 0.00% 65 669 0.00% 66 784 0.00% 67 762 0.00% 68 884 0.00% 69 910 0.00% 70 1082 0.00% 71 1246 0.00% 72 1308 0.00% 73 1607 0.00% 74 1557 0.00% 75 1696 0.00% 76 1918 0.00% 77 2060 0.00% 78 2146 0.00% 79 2425 0.00% 80 2560 0.00% 81 2987 0.00% 82 3531 0.01% 83 3841 0.01% 84 4107 0.01% 85 4578 0.01% 86 4738 0.01% 87 4949 0.01% 88 5635 0.01% 89 5735 0.01% 90 6782 0.01% 91 7461 0.01% 92 8340 0.01% 93 9598 0.02% 94 10619 0.02% 95 11585 0.02% 96 12975 0.02% 97 13968 0.02% 98 14710 0.02% 99 16097 0.03% 100 17350 0.03% 101 18640 0.03% 102 21583 0.04% 103 23716 0.04% 104 26080 0.04% 105 28392 0.05% 106 31325 0.05% 107 32991 0.05% 108 35620 0.06% 109 37788 0.06% 110 40594 0.07% 111 43840 0.07% 112 48309 0.08% 113 52430 0.09% 114 56697 0.09% 115 62856 0.10% 116 66213 0.11% 117 70998 0.12% 118 75752 0.12% 119 77789 0.13% 120 82799 0.14% 121 86986 0.14% 122 93633 0.15% 123 100377 0.16% 124 108472 0.18% 125 114287 0.19% 126 122906 0.20% 127 127530 0.21% 128 133106 0.22% 129 138420 0.23% 130 143925 0.24% 131 147688 0.24% 132 154792 0.25% 133 163606 0.27% 134 170538 0.28% 135 178051 0.29% 136 184951 0.30% 137 189790 0.31% 138 198309 0.32% 139 203994 0.33% 140 210012 0.34% 141 212346 0.35% 142 218931 0.36% 143 226122 0.37% 144 233799 0.38% 145 241147 0.39% 146 246836 0.40% 147 252582 0.41% 148 257854 0.42% 149 259241 0.42% 150 264208 0.43% 151 54635218 89.34% 61156109 reads passed initial QC criterion=sequence-density sequence-density=0.26 sequence-density-rank=1 fanout-score=3.16 fanout-score-rank=30 prefix-density=0.40 prefix-fanout=2.0 sequence=CACTTGCAGCCATTCTCAGCACC criterion=fanout-score sequence-density=0.03 sequence-density-rank=32 fanout-score=73.54 fanout-score-rank=1 prefix-density=0.24 prefix-fanout=10.3 sequence=CATCCTCATCACCTCCTTCAGCTGAAGGATTTGCACCAATGTCTACATCAACGGCTCCTTGAACAACCCACTTTCCTTCAACTTCCCACAGTATCCCATTCTCAATCTCCTTGTATGGGAACGAATCCGAGAGAAGCTCATCACCAGAGAGAAGATCTTGATAGACCAACATGATTGCGCAGAAGAGCTCCTCTCTTTCGGATCGACCCAAAGACAGACAAA criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=2.95 fanout-score-rank=33 prefix-density=0.35 prefix-fanout=2.9 sequence=ATGTACCCTGACTT criterion=fanout-score sequence-density=0.09 sequence-density-rank=15 fanout-score=345.21 fanout-score-rank=1 prefix-density=0.96 prefix-fanout=32.9 sequence=AAGAAGAAGAAA SRR26075335 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 20:53:07 Started mapping on | Feb 11 20:53:08 Finished on | Feb 11 21:06:45 Mapping speed, Million of reads per hour | 269.48 Number of input reads | 61156109 Average input read length | 298 UNIQUE READS: Uniquely mapped reads number | 52081412 Uniquely mapped reads % | 85.16% Average mapped length | 296.54 Number of splices: Total | 51313282 Number of splices: Annotated (sjdb) | 50038526 Number of splices: GT/AG | 50331291 Number of splices: GC/AG | 760005 Number of splices: AT/AC | 47513 Number of splices: Non-canonical | 174473 Mismatch rate per base, % | 0.42% Deletion rate per base | 0.03% Deletion average length | 3.00 Insertion rate per base | 0.02% Insertion average length | 2.52 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1391064 % of reads mapped to multiple loci | 2.27% Number of reads mapped to too many loci | 148070 % of reads mapped to too many loci | 0.24% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 11.71% % of reads unmapped: other | 0.61% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 7683633 7683633 7683633 N_multimapping 1391064 1391064 1391064 N_noFeature 1590462 51464787 1975206 N_ambiguous 520350 3309 286317 UnstrandedReadsAssigned:49970600 PositiveStrandReadsAssigned:613316 NegativeStrandReadsAssigned:49819889 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075335 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075335-trimmed-pair1.fastq SRR26075335-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 61,156,109 reads, 50,866,188 reads pseudoaligned [quant] estimated average fragment length: 222.602 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,169 rounds 52401 SRR26075335.ke.tsv 34699 SRR26075335.se.tsv 87100 total ==> SRR26075335.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1796.4 6178 66.7243 Potri.005G024800.1.v4.1 1035 813.398 3402 81.1465 Potri.004G059700.1.v4.1 961 739.415 0 0 Potri.007G009000.2.v4.1 1416 1194.4 0 0 Potri.003G141000.2.v4.1 2943 2721.4 2988.06 21.3028 Potri.016G087400.1.v4.1 270 82.924 3642.2 852.161 Potri.015G069301.1.v4.1 564 345.069 0 0 Potri.010G195200.1.v4.1 1773 1551.4 2518.88 31.5009 Potri.012G127500.1.v4.1 977 755.415 32317 830.011 ==> SRR26075335.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 308 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 926 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 2441 SRR26075335 completed mapping pipeline successfully