Starting /dee2/code/volunteer_pipeline.sh SRR26075336 current disk space = 3050685464576 free memory = 1486390344 SRR26075336 SRAfilesize 0c8a7c2d089c4701deef206a366f8bdb SRR26075336.sra SRR26075336.sra file validated SRR26075336 is paired end SRR26075336 is conventional basespace SRR26075336 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075336_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5535 37.0 37.0 37.0 37.0 37.0 2 36.6675 37.0 37.0 37.0 37.0 37.0 3 36.6395 37.0 37.0 37.0 37.0 37.0 4 36.6205 37.0 37.0 37.0 37.0 37.0 5 36.746 37.0 37.0 37.0 37.0 37.0 6 36.7285 37.0 37.0 37.0 37.0 37.0 7 36.7105 37.0 37.0 37.0 37.0 37.0 8 36.671 37.0 37.0 37.0 37.0 37.0 9 36.729 37.0 37.0 37.0 37.0 37.0 10-14 36.68465 37.0 37.0 37.0 37.0 37.0 15-19 36.6858 37.0 37.0 37.0 37.0 37.0 20-24 36.5951 37.0 37.0 37.0 37.0 37.0 25-29 36.5193 37.0 37.0 37.0 37.0 37.0 30-34 36.4785 37.0 37.0 37.0 37.0 37.0 35-39 36.423 37.0 37.0 37.0 37.0 37.0 40-44 36.3061 37.0 37.0 37.0 37.0 37.0 45-49 35.979400000000005 37.0 37.0 37.0 37.0 37.0 50-54 36.079699999999995 37.0 37.0 37.0 37.0 37.0 55-59 35.7273 37.0 37.0 37.0 37.0 37.0 60-64 35.7574 37.0 37.0 37.0 37.0 37.0 65-69 35.632799999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.8925 37.0 37.0 37.0 37.0 37.0 75-79 36.1362 37.0 37.0 37.0 37.0 37.0 80-84 36.0701 37.0 37.0 37.0 37.0 37.0 85-89 35.9879 37.0 37.0 37.0 37.0 37.0 90-94 35.9483 37.0 37.0 37.0 37.0 37.0 95-99 35.9833 37.0 37.0 37.0 37.0 37.0 100-104 35.919599999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.7846 37.0 37.0 37.0 37.0 37.0 110-114 35.6283 37.0 37.0 37.0 37.0 37.0 115-119 35.5483 37.0 37.0 37.0 37.0 37.0 120-124 35.5943 37.0 37.0 37.0 37.0 37.0 125-129 35.470800000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.4893 37.0 37.0 37.0 34.6 37.0 135-139 35.3074 37.0 37.0 37.0 32.2 37.0 140-144 35.243399999999994 37.0 37.0 37.0 34.6 37.0 145-149 35.1819 37.0 37.0 37.0 29.8 37.0 150-151 35.15325 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 3.0 23 3.0 24 7.0 25 7.0 26 6.0 27 9.0 28 18.0 29 25.0 30 27.0 31 43.0 32 93.0 33 151.0 34 149.0 35 400.0 36 2850.0 37 207.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.380331159056695 13.045659809332664 7.200200702458605 35.373808329152034 2 19.6 16.400000000000002 32.925 31.075000000000003 3 16.675 17.724999999999998 30.525000000000002 35.075 4 20.5 23.375 24.725 31.4 5 24.15 30.65 25.124999999999996 20.075000000000003 6 24.2 33.675 23.0 19.125 7 16.2 31.25 36.75 15.8 8 16.75 30.049999999999997 29.95 23.25 9 19.675 23.7 33.0 23.625 10-14 18.88594429721486 29.881494074703735 27.906395319765988 23.326166308315415 15-19 19.755 28.055000000000003 27.26 24.93 20-24 20.265 29.57 27.855 22.31 25-29 19.985 28.24 28.060000000000002 23.715 30-34 18.845 28.07 28.194999999999997 24.89 35-39 20.645 26.91 28.53 23.915 40-44 20.195 27.800000000000004 28.52 23.485 45-49 20.65 27.500000000000004 28.26 23.59 50-54 20.315 27.115000000000002 27.939999999999998 24.63 55-59 20.285 26.36 28.794999999999998 24.560000000000002 60-64 20.71 26.275 28.58 24.435000000000002 65-69 20.565 27.334999999999997 28.78 23.32 70-74 21.845 27.425 27.42 23.31 75-79 22.985 26.13 26.445 24.44 80-84 22.325 26.965 27.57 23.14 85-89 22.325 28.134999999999998 26.5 23.04 90-94 22.85 27.48 26.575 23.095 95-99 23.205000000000002 26.345000000000002 27.24 23.21 100-104 22.994999999999997 27.145000000000003 26.479999999999997 23.380000000000003 105-109 23.535 27.025 26.82 22.62 110-114 23.115 26.424999999999997 27.134999999999998 23.325000000000003 115-119 23.235 26.71 26.865 23.189999999999998 120-124 22.49 26.275 27.36 23.875 125-129 23.380000000000003 26.740000000000002 27.084999999999997 22.795 130-134 23.005 27.05 26.52 23.425 135-139 23.145 26.979999999999997 27.165 22.71 140-144 23.39 26.355 26.290000000000003 23.965 145-149 23.735 27.07 26.135 23.06 150-151 23.400000000000002 25.45 27.237499999999997 23.9125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.5 22 1.5 23 0.0 24 0.5 25 2.5 26 4.0 27 9.0 28 14.0 29 11.5 30 20.0 31 30.5 32 31.5 33 33.5 34 40.5 35 56.5 36 74.5 37 100.5 38 130.0 39 143.0 40 174.5 41 214.5 42 205.0 43 217.0 44 266.0 45 260.0 46 260.0 47 275.5 48 248.0 49 225.0 50 191.0 51 137.0 52 112.0 53 91.5 54 65.0 55 55.0 56 53.0 57 38.0 58 22.5 59 23.0 60 15.0 61 10.5 62 11.0 63 8.0 64 6.5 65 4.5 66 9.5 67 20.5 68 25.0 69 20.0 70 15.0 71 8.0 72 2.0 73 1.0 74 0.0 75 0.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.375 #Duplication Level Percentage of deduplicated Percentage of total 1 63.643892339544514 38.425 2 21.325051759834366 25.75 3 8.612836438923395 15.6 4 3.395445134575569 8.200000000000001 5 1.6149068322981366 4.875 6 0.6211180124223602 2.25 7 0.2898550724637681 1.225 8 0.2898550724637681 1.4000000000000001 9 0.041407867494824016 0.22499999999999998 >10 0.16563146997929606 2.0500000000000003 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTTATCTCGTAT 36 0.8999999999999999 TruSeq Adapter, Index 2 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTTATCGCGTAT 24 0.6 TruSeq Adapter, Index 2 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTTATCTCGTTT 12 0.3 TruSeq Adapter, Index 2 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTTATCGCGTTT 10 0.25 TruSeq Adapter, Index 2 (97% over 37bp) CTCATACTGTACAGTCCCCAACAAGTTCTCTCCATTCTCTGGATAATTGA 9 0.22499999999999998 No Hit CTGGGATGTAGGTGGCTGACTATAGTAATCATAACCCTGATTTTGCTGAT 8 0.2 No Hit GGTGCCACTCCGCAACACGTGCAGAACTGTGATTCGTGTGCCACCTGGGG 8 0.2 No Hit GGCATTGGCCAAAGTAGCGGCTTCAATTATTTCAGCCTCTGTTGCTGACT 8 0.2 No Hit GTCACGGTACAGATCTAGTTAGTCATAACCCATATCAAACACAACAAAAA 8 0.2 No Hit GTCGAATCCTAGCCCTGTAGTCAGTCTTCCCCTCTCTTCTTCTCTTAAAC 8 0.2 No Hit CTTTTTATTTACGCACATGGATTTGAGGGCTTTGCATACACCGTTCGATT 8 0.2 No Hit GGTTCGTCCATCCTCAAGCTGTTTGCCAGCAAAGATCAACCTCTGCTGAT 8 0.2 No Hit CCCAAACTCAACAGTACGTGAAAACTCAGGAGGCACTGGCTCAGTTGAAA 7 0.17500000000000002 No Hit CGTTGATTTAGGTCTGGCATCAATGATCATATCTTGTGCTTTTGTGATAG 7 0.17500000000000002 No Hit GTGAGCCTGAGAGGGGTTTGCAGGTATGCAGTGATGACATATTGGCTGGT 7 0.17500000000000002 No Hit CCAGCTTCTGCTTTAATTCCTTTTCCTTCTCATCAATTGCATCATATATC 7 0.17500000000000002 No Hit CCCCAAATAAGTATAAACCGGATGATAAGTTTCTGATGCTGGCAAAGGAC 7 0.17500000000000002 No Hit CCTAGGTTGTCCAGGCTGCCCTGGCTTTGGCATGTCATCCCTGCGTCTAA 7 0.17500000000000002 No Hit GCTTGGTACAGGCGGTCTCAAGGTGTTATTAACTATGCCTCCTATGATTG 7 0.17500000000000002 No Hit GCCATTTTCTTCACGTAAGGCTTAGTTCTCTTGTCAGTGGCTAGTTGGTG 6 0.15 No Hit CCCTGTATTTCTACCTCATTTCTTACAAATCTATTACAATCAGTACCGGA 6 0.15 No Hit ATCGAGGAAGAGTTGATAGAGGTCCCAAATGCCACGCTTATGCAAGCCTT 6 0.15 No Hit GTATTCACCAAAATGCGAATCTAATTGGAAACCATATAGCTTCTAATGAG 6 0.15 No Hit CTGGGAGGATGTCACATGGATTGGCGTTGAGCACATGCCTGATCTACATG 6 0.15 No Hit GGCTCATTCAATTCCAAAAATGGGAGTCTGATACACTGAGAGTTTGGGCT 6 0.15 No Hit CTTCAGGTTTCACAGCATCCTCATCTACAATTTCAAGAGGTGCATCCTCG 6 0.15 No Hit ATCGCGATCACTTGACACAAACACAATTTCAAAGTCGTCATCATTTCCTT 6 0.15 No Hit GAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATCC 6 0.15 No Hit CCTCCTAGCAAATTTCCTCTCCCTGCTAGCCTTGTTCTTTGCCCGCTTAG 6 0.15 No Hit GTGCAGAGGACAATGTCTTTAGGTGATGAGTTCGGCTGTTCGACCTGTTC 6 0.15 No Hit CATCCAGTCAAAACACAAACAATAGTTATAAAAAACAGCCACCTCAAGGT 6 0.15 No Hit GATTATCCCACAAACACTGCAATATTGCTGGTTACAAATACTAGAATCCC 6 0.15 No Hit CAGCATTTTCTCCAATCCTCACTCTAACAACAATCTTCTCAACCATCTTC 6 0.15 No Hit CCAGTTGAAGATTTTGTTCTCATGTCAAAACATTCGACAGCACCATCATC 6 0.15 No Hit ATCGTCAAAGTATCTAAAGTTCCTGAGGCCAAAGCTCCTGTCTGTCTCCT 5 0.125 No Hit CATCACTTCTAGTTATCGACAACTTCAATGAGGGTGGGCTCATAGTCGTT 5 0.125 No Hit CAGTGAAACTCTAATTATCGAACTCCTGGTATGACCTGAGAAGCTCAGTG 5 0.125 No Hit GGTTGATACATATGCAACTGCATTTGTTAACAAACTATGGTGGAGTGAGG 5 0.125 No Hit TACTGTCTAAATTCAACTTCTCCGAGCACCCCACATCAACACTGTAATTT 5 0.125 No Hit CTCCAACCCCGCTCCAAACCCACCATGAGGAGGGGCACCATATCTGAAAG 5 0.125 No Hit GGTCGAGATCGATGACCCCTTCTATTACTCCGTGCTAAGGGCTTCGTCGA 5 0.125 No Hit TATGTCTTCAAGTAAAACATCAGTCATGTTGTGTTCAGCGCAAGCTTGCT 5 0.125 No Hit CCTTGGGCGCTTCACTTGGAGAGAACATGTTAATTTTCTCATACTGTACA 5 0.125 No Hit CTTTCTTTGCCTTGCTCGCTAGCTTTGTTTTTCTTCTTGTGTTTTATATC 5 0.125 No Hit ACGGGCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGAC 5 0.125 No Hit GTATCATCCAAAACAAACCATGTAAATACATATTTACACTGAATGTATCT 5 0.125 No Hit GTGGAAGAGGAGCGAACCATTGAATAACTTCTTGATACCATTGAATAACT 5 0.125 No Hit CAATATTGCGATGAAGAGAAAACTGGACTCCCTTGGATTCACTCCCAAGC 5 0.125 No Hit CCCAGATCCTAGACCACTAAAACACACCCAAATACTAGATAAGATCCAAC 5 0.125 No Hit CCCCGCTGGAAACCTTGAAGTCGGATGGAGCTTCCTCTACATTTAGAACC 5 0.125 No Hit CCACAATACAAAGCTGCGAGATGGCGACGGTTTGTTGCGCCAACATCTTT 5 0.125 No Hit TTCTGGAATCTTGACCACATCATTTTGAGGAGTCCCCATCCAGTCACCAG 5 0.125 No Hit TGCGGAAATCCAGGGGAGACTAGGAGTAGTGAGGGATCCAGCATGACAAA 5 0.125 No Hit CTTTCAGTTGATGAACGTGAGAATCCACCTGTTTGAAGTCCACAACCTGC 5 0.125 No Hit GAGATGTTTATGTATGAAAGAGGAATTGAGGACGAGGAAGAAAAAACAAG 5 0.125 No Hit CTCGGGAGATGCCTCTCGCGCCATCTGTTGCTGCACCAAGTCATCTTCAT 5 0.125 No Hit CTCGCAGAGTTACATGACATGATGGCCGCAGCGGCGGCGGCGGCAACAGC 5 0.125 No Hit CCAGCAAGCAATGACATAAAAACAGTCCCGGGGATCATAAAGGTCTGCAT 5 0.125 No Hit CTCAAGGGGTTTTCGATTATTTGAGTAACTGCTCTCCTGAGAGGCCGGGC 5 0.125 No Hit CGGGATTTGTTTCCTTGATGGCAAGGCTATATAATTTCTCGAGAATGAAG 5 0.125 No Hit GCCGGATCATGAATATGAGGACATGGAACCTGTTTCAAGGAATTCGGATC 5 0.125 No Hit GGCTGAGTGGTTGATTGACAGAACAATATTAATCGGAATCGGAGACTCTT 5 0.125 No Hit CTCAAGCTGCTTGCCGGCAAATATCAGCCTCTGCTGGTCCGGGGGAATGC 5 0.125 No Hit GCCCATTTACAGACCAAGAAACAAACTAGCCACAACCCTTTTGTTCTTTC 5 0.125 No Hit CTCCATTCTCTGGATAATTGAAATCTCCAAATACCAGTTTACCTTTCTCC 5 0.125 No Hit CTTGTCAAGTACAACAGCAGTAGCAGTAGCACACCGTAGACAAACCTTGT 5 0.125 No Hit GTCCGAGAAGGTTGTGATTGTGATGAAGGTAGCGAGAGTTATTACCTGTA 5 0.125 No Hit CTGCTATGATCCTTGAATATTTTTATCAATTTAAGCCCTTGCTCTAACTT 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGTTGTTATCTCGGAT 5 0.125 TruSeq Adapter, Index 2 (97% over 37bp) AGGGCATCCTTTATTATCATAGCACTCAAAAGACTCACGATCGAGGAGAT 5 0.125 No Hit CAGGATTTTGTTCCTTGACAAAGCAATATCCCCAAGCGTATGCACCATCA 5 0.125 No Hit GCTCAATCTCTCTACATAATCTTTGATGTGAGTTTTTGTTTGGTTACACT 5 0.125 No Hit GCTACACACTGTACCCGTTCTCTATGAGAAGTTCGAGGACAAAATCGACC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.05 0.0 0.0 0.0 0.0 84-85 0.05 0.0 0.0 0.0 0.0 86-87 0.05 0.0 0.0 0.0 0.0 88-89 0.1 0.0 0.0 0.0 0.0 90-91 0.1 0.0 0.0 0.0 0.0 92-93 0.1 0.0 0.0 0.0 0.0 94-95 0.1125 0.0 0.0 0.0 0.0 96-97 0.175 0.0 0.0 0.0 0.0 98-99 0.2625 0.0 0.0 0.0 0.0 100-101 0.35 0.0 0.0 0.0 0.0 102-103 0.55 0.0 0.0 0.0 0.0 104-105 0.6125 0.0 0.0 0.0 0.0 106-107 0.625 0.0 0.0 0.0 0.0 108-109 0.8999999999999999 0.0 0.0 0.0 0.0 110-111 1.0 0.0 0.0 0.0 0.0 112-113 1.1 0.0 0.0 0.0 0.0 114-115 1.1749999999999998 0.0 0.0 0.0 0.0 116-117 1.4249999999999998 0.0 0.0 0.0 0.0 118-119 1.625 0.0 0.0 0.0 0.0 120-121 1.725 0.0 0.0 0.0 0.0 122-123 1.8375 0.0 0.0 0.0 0.0 124-125 2.1375 0.0 0.0 0.0 0.0 126-127 2.6 0.0125 0.0 0.0 0.0 128-129 2.925 0.025 0.0 0.0 0.0 130-131 3.6125 0.025 0.0 0.0 0.0 132-133 4.45 0.025 0.0 0.0 0.0 134-135 5.0875 0.025 0.0 0.0 0.0 136-137 5.7875 0.025 0.0 0.0 0.0 138-139 6.25 0.025 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCTGC 10 0.006830828 145.0 9 GCGGAAA 10 0.006830828 145.0 2 AGAGCTC 10 0.006830828 145.0 145 TGCGGAA 10 0.006830828 145.0 1 GAGCACA 30 0.0014437955 24.166668 140-144 AGAGCAC 35 0.0035366106 20.714287 140-144 TCGGAAG 60 0.004491891 14.500001 140-144 >>END_MODULE SRR26075336 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075336_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.9495 37.0 37.0 37.0 37.0 37.0 2 36.219 37.0 37.0 37.0 37.0 37.0 3 36.063 37.0 37.0 37.0 37.0 37.0 4 36.214 37.0 37.0 37.0 37.0 37.0 5 36.253 37.0 37.0 37.0 37.0 37.0 6 36.229 37.0 37.0 37.0 37.0 37.0 7 36.0545 37.0 37.0 37.0 37.0 37.0 8 36.199 37.0 37.0 37.0 37.0 37.0 9 36.1315 37.0 37.0 37.0 37.0 37.0 10-14 36.0588 37.0 37.0 37.0 37.0 37.0 15-19 35.9165 37.0 37.0 37.0 37.0 37.0 20-24 35.8332 37.0 37.0 37.0 37.0 37.0 25-29 35.6117 37.0 37.0 37.0 37.0 37.0 30-34 35.429100000000005 37.0 37.0 37.0 37.0 37.0 35-39 35.358 37.0 37.0 37.0 37.0 37.0 40-44 35.3066 37.0 37.0 37.0 37.0 37.0 45-49 35.09649999999999 37.0 37.0 37.0 37.0 37.0 50-54 34.9973 37.0 37.0 37.0 34.6 37.0 55-59 35.0229 37.0 37.0 37.0 32.2 37.0 60-64 35.18829999999999 37.0 37.0 37.0 37.0 37.0 65-69 35.0199 37.0 37.0 37.0 29.8 37.0 70-74 34.8311 37.0 37.0 37.0 25.0 37.0 75-79 34.800200000000004 37.0 37.0 37.0 25.0 37.0 80-84 34.8173 37.0 37.0 37.0 25.0 37.0 85-89 34.884100000000004 37.0 37.0 37.0 25.0 37.0 90-94 34.9353 37.0 37.0 37.0 27.4 37.0 95-99 35.041 37.0 37.0 37.0 27.4 37.0 100-104 35.0777 37.0 37.0 37.0 29.8 37.0 105-109 35.004599999999996 37.0 37.0 37.0 27.4 37.0 110-114 35.1134 37.0 37.0 37.0 29.8 37.0 115-119 34.9966 37.0 37.0 37.0 25.0 37.0 120-124 34.92229999999999 37.0 37.0 37.0 25.0 37.0 125-129 34.97260000000001 37.0 37.0 37.0 25.0 37.0 130-134 34.9701 37.0 37.0 37.0 25.0 37.0 135-139 34.8292 37.0 37.0 37.0 25.0 37.0 140-144 34.831050000000005 37.0 37.0 37.0 25.0 37.0 145-149 34.68044999999999 37.0 37.0 37.0 25.0 37.0 150-151 34.547375 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 4.0 14 8.0 15 18.0 16 21.0 17 14.0 18 7.0 19 7.0 20 7.0 21 12.0 22 9.0 23 19.0 24 26.0 25 34.0 26 24.0 27 32.0 28 27.0 29 15.0 30 25.0 31 35.0 32 46.0 33 71.0 34 189.0 35 647.0 36 2496.0 37 205.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.275 23.075000000000003 10.424999999999999 20.225 2 31.95 25.674999999999997 25.75 16.625 3 25.825 29.549999999999997 27.775 16.85 4 28.375 31.474999999999998 20.925 19.225 5 29.675 34.575 20.25 15.5 6 27.800000000000004 36.1 21.55 14.549999999999999 7 25.7 21.675 34.525 18.099999999999998 8 26.25 24.2 25.124999999999996 24.425 9 25.4 25.95 27.3 21.349999999999998 10-14 27.534999999999997 28.765 23.54 20.16 15-19 28.560000000000002 27.245 24.945 19.25 20-24 27.445000000000004 27.43 25.230000000000004 19.895 25-29 27.74 27.779999999999998 24.62 19.86 30-34 26.724999999999998 28.544999999999998 24.755 19.975 35-39 27.975 27.67 25.185000000000002 19.17 40-44 26.655 26.99 26.384999999999998 19.97 45-49 26.955000000000002 27.575 25.240000000000002 20.23 50-54 24.605 27.04 28.110000000000003 20.244999999999997 55-59 25.785000000000004 28.02 26.52 19.675 60-64 27.084999999999997 27.015 25.715 20.185 65-69 26.71 28.555000000000003 25.669999999999998 19.064999999999998 70-74 25.2 28.854999999999997 26.165 19.78 75-79 24.79 29.134999999999998 26.305 19.77 80-84 25.679999999999996 28.799999999999997 25.779999999999998 19.74 85-89 27.025 27.045 26.755000000000003 19.175 90-94 26.095000000000002 27.88 26.090000000000003 19.935 95-99 25.974999999999998 28.599999999999998 25.835 19.59 100-104 25.840000000000003 27.700000000000003 25.85 20.61 105-109 28.09 26.884999999999998 25.045 19.98 110-114 26.740000000000002 27.42 25.814999999999998 20.025000000000002 115-119 26.07 27.905 26.224999999999998 19.8 120-124 26.55 28.084999999999997 25.82 19.545 125-129 26.935 28.09 26.009999999999998 18.965 130-134 26.729999999999997 28.64 25.324999999999996 19.305 135-139 27.095000000000002 28.215 26.314999999999998 18.375 140-144 27.646382319115958 27.996399819990998 25.38126906345317 18.975948797439873 145-149 27.44911736760514 28.05420813121968 25.25378806821023 19.242886432964944 150-151 29.778722340292536 28.366045755719465 23.615451931491435 18.23977997249656 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 1.0 7 0.0 8 1.0 9 2.0 10 2.0 11 1.0 12 0.5 13 1.0 14 1.5 15 2.0 16 1.5 17 0.5 18 0.0 19 0.0 20 0.0 21 0.0 22 1.0 23 4.5 24 5.5 25 3.5 26 4.5 27 4.0 28 6.0 29 6.0 30 3.5 31 10.5 32 13.5 33 11.5 34 31.5 35 54.5 36 56.0 37 72.5 38 95.0 39 135.0 40 194.0 41 219.5 42 236.0 43 243.5 44 244.5 45 261.0 46 288.5 47 307.5 48 252.0 49 198.5 50 176.5 51 136.5 52 118.0 53 108.0 54 84.5 55 53.5 56 38.5 57 34.0 58 22.5 59 16.0 60 14.5 61 14.5 62 13.0 63 6.5 64 4.5 65 5.0 66 4.5 67 4.0 68 3.5 69 2.0 70 2.5 71 3.5 72 4.0 73 3.0 74 1.5 75 2.0 76 3.0 77 4.0 78 5.5 79 5.0 80 4.0 81 4.0 82 6.5 83 10.5 84 9.0 85 7.5 86 7.5 87 10.0 88 8.5 89 6.5 90 8.0 91 5.0 92 4.5 93 3.5 94 1.0 95 2.0 96 2.5 97 1.5 98 1.5 99 2.0 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.015 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.425 #Duplication Level Percentage of deduplicated Percentage of total 1 66.15939126952343 41.3 2 20.104124949939926 25.1 3 7.889467360833 14.774999999999999 4 3.364036844213056 8.4 5 1.0812975570684822 3.375 6 0.6407689227072487 2.4 7 0.32038446135362436 1.4000000000000001 8 0.2803364036844213 1.4000000000000001 9 0.040048057669203045 0.22499999999999998 >10 0.12014417300760913 1.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 41 1.0250000000000001 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 13 0.325 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGT 11 0.27499999999999997 No Hit ATTTTGGGAATGCTGGAAGCTTGGATAAGAAAACAATGGTTCCAGGTGAT 9 0.22499999999999998 No Hit GAGCCATCATCTGGGCGGGTGATGATTGATGGGAAGGACATTAGGAAATA 8 0.2 No Hit TATTAGCCGTCTCCCCCTCCTTCCCCTCGTCCCCCTCTAACAGATCAAGC 8 0.2 No Hit AATCTCTCAAGATGCAAATTTTTGTGAAAACTCTCACTGGCAAGACCATC 8 0.2 No Hit GGATGAGGTGCATCTGTCTCTGTCTTTGCTTTCAATTCGGTATTATGAGA 8 0.2 No Hit GCTACATGCAGGCTGGAGCATACCCTGGTGCATCAGCACAGTATAATACG 8 0.2 No Hit CAAAAACCCAGATGAGCAAGTTAGAAAACACAGAAAATCAGTGCACCTGG 8 0.2 No Hit ATGAAGGTTCATCTCCCACCCAAGAGCAGGAAGCTGTTTCAAAGAATGAG 8 0.2 No Hit GCATGTGATTTTGCAACTCATGTTTTCTGCCACCCATTAGCCCTTTGCCA 7 0.17500000000000002 No Hit ACTGGGGCCAATGGGGTGCCTGTATCGGAATCGCAGCACTATCTTGGCCA 7 0.17500000000000002 No Hit GTCAGCTCGAGAGATTTCACAGTGCAACCTCACTCACATTTGCCATCCAA 7 0.17500000000000002 No Hit TATTATTCAATGGTGGTTATACACAGCAGCAGATAAGTGGTATTGGTACA 7 0.17500000000000002 No Hit CGTCATACAAAGGAAATGTTACCGACAAAGCTAGCCGTCCCTCTTCTAAT 7 0.17500000000000002 No Hit AAACTGTACGTCAAGATGCAGTACTGTGTCTCTTGTGCTATTCACTCCCG 7 0.17500000000000002 No Hit AGGGGTTGTATGCTTGGGTTATTGCGAATTATGCGTTGGGTACTCTTGGT 7 0.17500000000000002 No Hit CCCTGAACTCCCCACTTACCATGAAGCTTGTCCCCAAGCCAACTGTGGTG 7 0.17500000000000002 No Hit GGCAATTTGTGGTCTCCTCCCTAAGCTAGTGTACCTGAACAAGCAACCCA 6 0.15 No Hit CTTAATCAATTTGATACGAGTATGATCCCAATCCTCTTCTTTCCTCTTTC 6 0.15 No Hit GCTGTCCAATATGTGAGGAATGACAGAGGCACACCCATGGTTCTAGACAA 6 0.15 No Hit CGATGAGAGCTCAAGCTTTCATATGCAGTAAACTTGATTTACTGTGGTTT 6 0.15 No Hit CTCACCTTGCAACACACAGTCTCTCACTAATGGCAACATCAACTGCAGCG 6 0.15 No Hit AATGAGTTGCAAATTCTGGTTGATGGAGAGGAGAAGAAGAAGGCGAACTT 6 0.15 No Hit GTTGGTCTTGTGACTTGCTCTGTGCTGCCTCATCTTCAGATTTATATCGG 6 0.15 No Hit GGAGAAGCATGAGAGGCAGACCCTAACCAATTTGCTGATTTGCCATAATA 6 0.15 No Hit AGATGATTCTGTTTGATGCAGTGTTCATCTTCGAGCTCTTCTTGAAGAAT 6 0.15 No Hit GATTAGGTAAGGATTTCCCCGTGAACACCAACTCTGGGATACACAGATGT 6 0.15 No Hit GGATGGTTTCATTATCAGAAAGCCAACTAAGATTCACTCTCGGTCCCGCG 6 0.15 No Hit GTATCCACCCGGAAGAGGAAGGAGATTGTCGAGCGAGCAGCGCAGTTGGA 6 0.15 No Hit GGTCTTGTAAGGAGAAGAGCCCAGAAATCCGTAGGATTTTGAAGATCCAT 6 0.15 No Hit GCTCAAAGCAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATA 6 0.15 No Hit AAAAGGAATACCTTGGTTACTGGTGGTGTAAGGGGAACTCTTCCATGGAT 6 0.15 No Hit AGGGGATCCCACCAGACCAGCAGAGGCTTATTTTTGCTGGCAAGCAGCTT 6 0.15 No Hit TGCTAACACTCGTAAAAGGGAGATCGCTGCTTTCTTGGGCCAAACCTCTC 5 0.125 No Hit TTTCAAATGTAGCTGGTAAAACAAGTTCAGACTCAGGAGTTAGCTACTTA 5 0.125 No Hit AGCGAAGACAGCGATGACGACGATGACGATGCGGCCGACGATGAAGACGC 5 0.125 No Hit AGAGCGAGCCGCTAGCTAGCTGCTCTGCAGGACGCTGTTCTTCTTGAGAA 5 0.125 No Hit ATTGGCTCTGTTGTTTGGTTCTTTGGGTTTTAAGTGATTTAGCCTTCGCA 5 0.125 No Hit CAGGATAGACGAAGTTGTGGTATTCCATCCCCTTGAGAAGGCTCAGACGC 5 0.125 No Hit GGGATTGATTCTGTCATTTGTGTGGCTGTTAACGATCCATATACTATGAA 5 0.125 No Hit GGACCAATGGCACCAGCAGATAGCAAGACCTGCTCGTCAATTCATGGATA 5 0.125 No Hit GTGAAGGCTATCGAGAAGGTTGGATCTGGAAGCGGCAAGACTTCCAAGCC 5 0.125 No Hit CACCATCTGCAGGCCGTGACCGTGCCCGTGTTATCTTGACCAGTTACAAT 5 0.125 No Hit CAACTCAATTCATGGATGTTGTGAGTCAGTTGCAGAGACAGCTTGCCGAT 5 0.125 No Hit AGCGTGTCCATGTACCAGAATTCTTGGTAGAACGTGCACATGCATGTGGG 5 0.125 No Hit AGTTTATTGTGGAATTTGACAAAGAAATATTTAACAGTTGGGGTCATAGG 5 0.125 No Hit GGCCTTCCAGGCCAAGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCT 5 0.125 No Hit AGAGAATTGATGATCTTGTTCCAGAATGAAGCTATAGAGAAAATTAGCAG 5 0.125 No Hit AGATGATCAATCAAGTGCAGTGATTATTGGAAATCCCCGTTCCTCGGATT 5 0.125 No Hit GATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGT 5 0.125 No Hit CTGAGGTCAACCCTAATTTAGGGTTTCAAGTAAAATCTGATTCTATTTCT 5 0.125 No Hit CAGACTTTCTATAATTTCTTTTCATTTCCATCTTCATCTTCAAGAAATAT 5 0.125 No Hit CCTGGATTGAAAGCTGGTGTCAGGTTCCGCAATGATGTTCCCACTGGTGG 5 0.125 No Hit AAGAAATGTTCAAGGCTATGGATACTGATAACAGTGGTGCAATCACATTT 5 0.125 No Hit CTCAATCCTAAGAGTTCAAATAAGCGAGCTCAGCAATAGATTGGAGTCTT 5 0.125 No Hit CTTGAAGAAAAGCCAACTCACGCGAAGAAGAGGAGAGAAAGCAAAGGCAA 5 0.125 No Hit ATGGGATCTTGCTCAGAACCTTAGCATCGAAGACCGCATATTGCCTTTTG 5 0.125 No Hit GGCACATTGTGAAGGATGTCCCCAACAGTTTCTTGAAGCATATTACCCTC 5 0.125 No Hit ACGGGTTAGAGTTGCTCGTGCCGGGTCGAGTATTGCTAGGTGTCAAGTAC 5 0.125 No Hit GGAGGACATGAACTTAGCAAAACCACTGGTAATGCTGGCGGCAGAGTAGC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.025 0.0 0.0 0.0 0.0 88-89 0.075 0.0 0.0 0.0 0.0 90-91 0.075 0.0 0.0 0.0 0.0 92-93 0.075 0.0 0.0 0.0 0.0 94-95 0.0875 0.0 0.0 0.0 0.0 96-97 0.15 0.0 0.0 0.0 0.0 98-99 0.2375 0.0 0.0 0.0 0.0 100-101 0.325 0.0 0.0 0.0 0.0 102-103 0.55 0.0 0.0 0.0 0.0 104-105 0.6125 0.0 0.0 0.0 0.0 106-107 0.625 0.0 0.0 0.0 0.0 108-109 0.925 0.0 0.0 0.0 0.0 110-111 1.0499999999999998 0.0 0.0 0.0 0.0 112-113 1.1375 0.0 0.0 0.0 0.0 114-115 1.2000000000000002 0.0 0.0 0.0 0.0 116-117 1.4249999999999998 0.0 0.0 0.0 0.0 118-119 1.625 0.0 0.0 0.0 0.0 120-121 1.725 0.0 0.0 0.0 0.0 122-123 1.8375 0.0 0.0 0.0 0.0 124-125 2.1375 0.0 0.0 0.0 0.0 126-127 2.65 0.0 0.0 0.0 0.0 128-129 2.9749999999999996 0.0 0.0 0.0 0.0 130-131 3.6625 0.0 0.0 0.0 0.0 132-133 4.525 0.0 0.0 0.0 0.0 134-135 5.1625 0.0 0.0 0.0 0.0 136-137 5.887499999999999 0.0 0.0 0.0 0.0 138-139 6.3375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCACAC 10 0.006830828 145.0 145 TCGGAAG 55 0.0025160722 15.818182 140-144 >>END_MODULE Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422762 spots for SRR26075336.sra Written 3422762 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra Read 3422759 spots for SRR26075336.sra Written 3422759 spots for SRR26075336.sra SRR ids: ['SRR26075336.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_3joho43h SRR26075336.sra spots: 68455183 blocks: [[1, 3422759], [3422760, 6845518], [6845519, 10268277], [10268278, 13691036], [13691037, 17113795], [17113796, 20536554], [20536555, 23959313], [23959314, 27382072], [27382073, 30804831], [30804832, 34227590], [34227591, 37650349], [37650350, 41073108], [41073109, 44495867], [44495868, 47918626], [47918627, 51341385], [51341386, 54764144], [54764145, 58186903], [58186904, 61609662], [61609663, 65032421], [65032422, 68455183]] SRR26075336 file size 25289854 SRR26075336 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075336 SRR26075336_1.fastq SRR26075336_2.fastq Input file: SRR26075336_1.fastq Paired file: SRR26075336_2.fastq trimmed: SRR26075336-trimmed-pair1.fastq, SRR26075336-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:18:59 2025 >> started Wed Feb 12 02:20:17 2025 >> done (77.868s) 68455183 read pairs processed; of these: 599 ( 0.00%) short read pairs filtered out after trimming by size control 1506620 ( 2.20%) empty read pairs filtered out after trimming by size control 66947964 (97.80%) read pairs available; of these: 7312859 (10.92%) trimmed read pairs available after processing 59635105 (89.08%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 41 0.00% 19 56 0.00% 20 62 0.00% 21 93 0.00% 22 105 0.00% 23 121 0.00% 24 163 0.00% 25 145 0.00% 26 161 0.00% 27 193 0.00% 28 187 0.00% 29 165 0.00% 30 218 0.00% 31 172 0.00% 32 205 0.00% 33 210 0.00% 34 225 0.00% 35 215 0.00% 36 213 0.00% 37 213 0.00% 38 328 0.00% 39 279 0.00% 40 320 0.00% 41 292 0.00% 42 237 0.00% 43 279 0.00% 44 293 0.00% 45 332 0.00% 46 296 0.00% 47 350 0.00% 48 388 0.00% 49 400 0.00% 50 469 0.00% 51 464 0.00% 52 549 0.00% 53 581 0.00% 54 606 0.00% 55 845 0.00% 56 718 0.00% 57 729 0.00% 58 1272 0.00% 59 912 0.00% 60 1234 0.00% 61 995 0.00% 62 1081 0.00% 63 1369 0.00% 64 1186 0.00% 65 1188 0.00% 66 1142 0.00% 67 1230 0.00% 68 1494 0.00% 69 1529 0.00% 70 1744 0.00% 71 1902 0.00% 72 1991 0.00% 73 2216 0.00% 74 2280 0.00% 75 2514 0.00% 76 2762 0.00% 77 2864 0.00% 78 2956 0.00% 79 3172 0.00% 80 3707 0.01% 81 3644 0.01% 82 4522 0.01% 83 4659 0.01% 84 5380 0.01% 85 5633 0.01% 86 6071 0.01% 87 6737 0.01% 88 7193 0.01% 89 7804 0.01% 90 8448 0.01% 91 9180 0.01% 92 10407 0.02% 93 11267 0.02% 94 12732 0.02% 95 13871 0.02% 96 14412 0.02% 97 15642 0.02% 98 17447 0.03% 99 18870 0.03% 100 19768 0.03% 101 21434 0.03% 102 24593 0.04% 103 26363 0.04% 104 29201 0.04% 105 31026 0.05% 106 34409 0.05% 107 36239 0.05% 108 39727 0.06% 109 43162 0.06% 110 44406 0.07% 111 49169 0.07% 112 52472 0.08% 113 56443 0.08% 114 61118 0.09% 115 67423 0.10% 116 71370 0.11% 117 76731 0.11% 118 81520 0.12% 119 84726 0.13% 120 90495 0.14% 121 96608 0.14% 122 102401 0.15% 123 108932 0.16% 124 118278 0.18% 125 124095 0.19% 126 132050 0.20% 127 140174 0.21% 128 146351 0.22% 129 153053 0.23% 130 158147 0.24% 131 163872 0.24% 132 173420 0.26% 133 180858 0.27% 134 188012 0.28% 135 199876 0.30% 136 206406 0.31% 137 214547 0.32% 138 222214 0.33% 139 226515 0.34% 140 234356 0.35% 141 243986 0.36% 142 246388 0.37% 143 252726 0.38% 144 266756 0.40% 145 274416 0.41% 146 281082 0.42% 147 287281 0.43% 148 290148 0.43% 149 295819 0.44% 150 303720 0.45% 151 59635105 89.08% 66947964 reads passed initial QC criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=3.84 fanout-score-rank=18 prefix-density=0.34 prefix-fanout=3.1 sequence=TGGTGATGGGAAGCCAGAAAACTTCCTTGGGCGCTTCACTTGGAGAGAACATGTTAATTTTCTCATACTGTACAGTCCCCA criterion=fanout-score sequence-density=0.04 sequence-density-rank=38 fanout-score=140.03 fanout-score-rank=1 prefix-density=0.37 prefix-fanout=14.8 sequence=CTCCTTCAAAGAAGGGGCAAAGTACAAGAATGTTGGATCGGTAGCACCTTCTTTGTAATAAGCAAAGACCAAACAACCATCATCATGCATGCTCTCCCCCACAAAGAATTGCAAGTCCTTGATTTTTGAAAGCAAGAACTTGGTTGCTCCCTCAATGTTCTTTCTAAAATGTTCCTTCTGGTCCTCATCAAGTTTCTCCGACAGATTCTTGATAAATTTCTTAATCTGTGTAAGAAACTGCTTCTTGTCAAATGGAGGTTGCTCCTGGAGCCTAAATGTGTCAACGATGTCAACAACCTTGGCAGCTTGGTCATCAACACCCTCATCCTCATCACCTCCTTCAGCTGAAGGATTTGCACCAATGTCTACATCAACGGCTCCTTGAACAACCCACTTTCCTTCAACTTCCCACAGTATCCCATTCTCAATCTCCTTGTATGGGAACGAATCCGAGAGAAGCTCATCACCAGAGAGAAGATCTTGATA criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=2.51 fanout-score-rank=20 prefix-density=0.32 prefix-fanout=2.5 sequence=ATGTACCCTGACTTAGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=28 fanout-score=252.27 fanout-score-rank=1 prefix-density=0.16 prefix-fanout=12.2 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGG SRR26075336 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:20:58 Started mapping on | Feb 12 02:20:59 Finished on | Feb 12 02:32:17 Mapping speed, Million of reads per hour | 355.48 Number of input reads | 66947964 Average input read length | 298 UNIQUE READS: Uniquely mapped reads number | 58640651 Uniquely mapped reads % | 87.59% Average mapped length | 296.33 Number of splices: Total | 54392715 Number of splices: Annotated (sjdb) | 53030889 Number of splices: GT/AG | 53396154 Number of splices: GC/AG | 750831 Number of splices: AT/AC | 52879 Number of splices: Non-canonical | 192851 Mismatch rate per base, % | 0.42% Deletion rate per base | 0.03% Deletion average length | 3.07 Insertion rate per base | 0.02% Insertion average length | 2.56 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1893936 % of reads mapped to multiple loci | 2.83% Number of reads mapped to too many loci | 258187 % of reads mapped to too many loci | 0.39% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.46% % of reads unmapped: other | 0.73% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6413377 6413377 6413377 N_multimapping 1893936 1893936 1893936 N_noFeature 1459918 57955166 1794464 N_ambiguous 718800 4392 365213 UnstrandedReadsAssigned:56461933 PositiveStrandReadsAssigned:681093 NegativeStrandReadsAssigned:56480974 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075336 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075336-trimmed-pair1.fastq SRR26075336-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 66,947,964 reads, 57,893,417 reads pseudoaligned [quant] estimated average fragment length: 218.818 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,275 rounds 52401 SRR26075336.ke.tsv 34699 SRR26075336.se.tsv 87100 total ==> SRR26075336.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1800.18 7541 55.6489 Potri.005G024800.1.v4.1 1035 817.182 3908 63.5301 Potri.004G059700.1.v4.1 961 743.182 294 5.25528 Potri.007G009000.2.v4.1 1416 1198.18 0 0 Potri.003G141000.2.v4.1 2943 2725.18 2336 11.3873 Potri.016G087400.1.v4.1 270 82.194 8711.02 1407.9 Potri.015G069301.1.v4.1 564 347.434 0 0 Potri.010G195200.1.v4.1 1773 1555.18 554 4.7323 Potri.012G127500.1.v4.1 977 759.182 25820 451.808 ==> SRR26075336.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 2026 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 1206 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 31 Potri.001G416900.v4.1 1 Potri.001G452600.v4.1 1251 SRR26075336 completed mapping pipeline successfully