Starting /dee2/code/volunteer_pipeline.sh SRR26075337 current disk space = 3052943015936 free memory = 1573380152 SRR26075337 SRAfilesize 81521bcf147a253faec5f2f7e103630d SRR26075337.sra SRR26075337.sra file validated SRR26075337 is paired end SRR26075337 is conventional basespace SRR26075337 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075337_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.61125 37.0 37.0 37.0 37.0 37.0 2 36.5275 37.0 37.0 37.0 37.0 37.0 3 36.584 37.0 37.0 37.0 37.0 37.0 4 36.603 37.0 37.0 37.0 37.0 37.0 5 36.6845 37.0 37.0 37.0 37.0 37.0 6 36.6545 37.0 37.0 37.0 37.0 37.0 7 36.5785 37.0 37.0 37.0 37.0 37.0 8 36.657 37.0 37.0 37.0 37.0 37.0 9 36.688 37.0 37.0 37.0 37.0 37.0 10-14 36.5721 37.0 37.0 37.0 37.0 37.0 15-19 36.5591 37.0 37.0 37.0 37.0 37.0 20-24 36.5003 37.0 37.0 37.0 37.0 37.0 25-29 36.378699999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.3264 37.0 37.0 37.0 37.0 37.0 35-39 36.2807 37.0 37.0 37.0 37.0 37.0 40-44 36.220000000000006 37.0 37.0 37.0 37.0 37.0 45-49 36.1885 37.0 37.0 37.0 37.0 37.0 50-54 36.0537 37.0 37.0 37.0 37.0 37.0 55-59 36.0884 37.0 37.0 37.0 37.0 37.0 60-64 35.998000000000005 37.0 37.0 37.0 37.0 37.0 65-69 36.0027 37.0 37.0 37.0 37.0 37.0 70-74 35.868500000000004 37.0 37.0 37.0 37.0 37.0 75-79 35.8985 37.0 37.0 37.0 37.0 37.0 80-84 35.8606 37.0 37.0 37.0 37.0 37.0 85-89 35.7291 37.0 37.0 37.0 37.0 37.0 90-94 35.7535 37.0 37.0 37.0 37.0 37.0 95-99 35.7661 37.0 37.0 37.0 37.0 37.0 100-104 35.6301 37.0 37.0 37.0 37.0 37.0 105-109 35.5801 37.0 37.0 37.0 37.0 37.0 110-114 35.4663 37.0 37.0 37.0 37.0 37.0 115-119 35.376599999999996 37.0 37.0 37.0 34.6 37.0 120-124 35.4862 37.0 37.0 37.0 37.0 37.0 125-129 35.32790000000001 37.0 37.0 37.0 37.0 37.0 130-134 35.245 37.0 37.0 37.0 32.2 37.0 135-139 35.0918 37.0 37.0 37.0 29.8 37.0 140-144 34.8945 37.0 37.0 37.0 25.0 37.0 145-149 34.976 37.0 37.0 37.0 25.0 37.0 150-151 34.658249999999995 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 5.0 23 5.0 24 9.0 25 10.0 26 20.0 27 16.0 28 26.0 29 33.0 30 39.0 31 54.0 32 72.0 33 96.0 34 158.0 35 429.0 36 2841.0 37 185.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.354297168629415 12.728639438737158 6.514657980456026 34.4024054121774 2 20.9 13.625000000000002 33.900000000000006 31.574999999999996 3 18.925 21.5 26.674999999999997 32.9 4 23.925 27.3 25.174999999999997 23.599999999999998 5 25.724999999999998 29.75 25.074999999999996 19.45 6 21.425 32.1 27.35 19.125 7 16.45 24.925 40.675 17.95 8 19.925 26.375 29.65 24.05 9 21.099999999999998 22.35 33.074999999999996 23.474999999999998 10-14 21.117111711171116 28.77287728772877 27.06770677067707 23.042304230423042 15-19 21.13 26.334999999999997 28.415000000000003 24.12 20-24 21.46 26.974999999999998 26.939999999999998 24.625 25-29 22.42 27.125 26.619999999999997 23.835 30-34 21.475 26.16 27.439999999999998 24.925 35-39 21.165 27.38 26.39 25.064999999999998 40-44 22.29 26.924999999999997 26.765 24.02 45-49 20.76 24.740000000000002 28.395 26.105 50-54 21.3 28.04 25.91 24.75 55-59 21.185000000000002 26.265 27.794999999999998 24.755 60-64 20.4 27.16 27.16 25.28 65-69 21.105 26.705000000000002 27.71 24.48 70-74 21.584999999999997 26.135 27.375 24.905 75-79 21.87 26.375 27.325 24.43 80-84 21.125 27.46 26.185000000000002 25.230000000000004 85-89 21.015 26.640000000000004 26.51 25.835 90-94 21.325 26.979999999999997 26.845000000000002 24.85 95-99 21.395 26.669999999999998 27.35 24.585 100-104 21.69 26.08 27.13 25.1 105-109 21.675 26.77 27.51 24.044999999999998 110-114 21.12 27.744999999999997 27.215 23.919999999999998 115-119 22.384999999999998 26.705000000000002 26.93 23.98 120-124 21.775 27.79 25.865 24.57 125-129 21.565 27.08 26.419999999999998 24.935 130-134 21.52 26.705000000000002 26.915 24.86 135-139 22.095000000000002 26.395000000000003 27.26 24.25 140-144 22.415 26.935 26.19 24.46 145-149 21.88 28.895 25.1 24.125 150-151 21.6125 28.0625 25.025 25.3 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 1.0 2 0.5 3 1.0 4 1.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 2.5 13 2.5 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 1.5 21 2.5 22 2.0 23 2.5 24 4.5 25 4.0 26 4.0 27 5.0 28 9.0 29 20.5 30 24.0 31 19.5 32 20.0 33 22.0 34 28.0 35 42.0 36 59.5 37 77.5 38 102.0 39 128.5 40 158.0 41 176.5 42 180.5 43 191.5 44 202.0 45 239.5 46 244.5 47 222.5 48 237.0 49 226.0 50 191.0 51 160.5 52 134.5 53 114.0 54 112.5 55 104.5 56 79.5 57 69.0 58 68.0 59 67.0 60 58.5 61 37.0 62 19.5 63 18.0 64 16.5 65 17.5 66 16.5 67 13.0 68 9.0 69 5.5 70 11.0 71 7.0 72 0.0 73 1.5 74 1.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.675000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 63.12319736299958 38.3 2 21.796456530696336 26.450000000000003 3 8.0758137618459 14.7 4 3.996703749484961 9.700000000000001 5 1.4009064688916357 4.25 6 0.9476720230737536 3.45 7 0.3296250515039143 1.4000000000000001 8 0.16481252575195715 0.8 9 0.08240626287597858 0.44999999999999996 >10 0.08240626287597858 0.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGACTTCTCGCG 10 0.25 No Hit CTAATTTCTTCAAAGTAACAGCACCGGAGGCACGACCCGGCCAGTTAAGG 10 0.25 No Hit CAATGCAATTTTTAGTCTCGTAGGCCAATCCATGGTGGGTCGCCCCTTCC 9 0.22499999999999998 No Hit GCCCCTATACCCAAGTCAGACGAACGATTTGCACGTCAGTATCGCTGCGG 9 0.22499999999999998 No Hit AGCACATGCAACATCTTGTGTCTCCCCTAGTTTTTGACTTAACCTCCATG 8 0.2 No Hit CTTGTTACGACTTCTCCTTCCTCTAAATGATAAGGTTCAGTGGACTTCTC 8 0.2 No Hit CTCTTGAGGTACTCACTAAAGTGAGACTGGTATTTCTCAGGTTCATCCTC 8 0.2 No Hit CCGACTTTGCCAAAGTAACCAGGATGGTACTTGTCGAATAGAATCCGGTG 8 0.2 No Hit GCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGA 7 0.17500000000000002 No Hit GCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGT 7 0.17500000000000002 No Hit GCCCTTTGTCCATCAAAGGCAAGTAAATGTAGGATTCAGTGAACATGAAT 7 0.17500000000000002 No Hit GTCAGTACCATATATTCCATTGTTTATATCTGGCAGTGTCACATCAAGAG 7 0.17500000000000002 No Hit ATGCAGATAATCTCCTTATTGTTTCTTCCAAATTTATTAATTTGCGTCTA 7 0.17500000000000002 No Hit CCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCA 7 0.17500000000000002 No Hit CGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCA 7 0.17500000000000002 No Hit TCTAAAGCTTTTGTTGAGTTTGCCATATCTTGATAGAACTGGGTTAGATG 7 0.17500000000000002 No Hit CCATGCTAGTAGTCATCGCCCCTCGATATGACCTCTTTGCAGGTTGGCCG 6 0.15 No Hit GGAGGTGGTGCTGGCATTCCAGGTCTTGGTGGAGCAGGTGGTCCTCTCAT 6 0.15 No Hit GTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACACCTGCG 6 0.15 No Hit CAGTTCCTTGTTATTTTCGGGCAGATGTTGGTAATAAGCTCCAGCAGCAA 6 0.15 No Hit GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT 6 0.15 No Hit CCAAGATCTCTCATCAGAATAATTTCTCTCTCCCCAACCGTCGAGATCAC 6 0.15 No Hit CCCAAACAACCCGACTCGCAGACAGCGCCTCGTGGTGCGGCAGGGTCCAG 6 0.15 No Hit GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT 6 0.15 No Hit GCAGGGATATTATTTTATTGCATTGTATTTCATCTTACCCAACCCCTTAT 6 0.15 No Hit CCTGGATTGTCTGCATGAAAACGAATTGCCACCCAGCCACCAGAAGGCAC 6 0.15 No Hit CGTGACTTAAAAACCCACTAGGAGAAATGAATCCACCAGAAAGGATTTGA 6 0.15 No Hit CTCTTCTTCCAGACGTGAAAAGTCAATTGCCCGTTGACCTGTAACAAGCT 6 0.15 No Hit GACCTTGTTGCCCATGGCATCGTAAGTATCCTCCTCCTCACCATCCTTCT 6 0.15 No Hit GGTCTTTAAAACGGAACTGGTTTACCCTTAGGGTAATCGACATAAACCCT 6 0.15 No Hit ATGCCGTTTGCTTTCAGGCCATCAGGAACTTCGAAGCCATGAAATGCCCA 6 0.15 No Hit GGGCATCAAGTGCTTGAACTTGCCAGGCATTGTCTTGCACAGAATTGGGA 6 0.15 No Hit GTAGCAGAGAAGACAGTGTAGACAAGAACAAAGGTACCGACTATCTCCGC 6 0.15 No Hit CCTTGGCTGTGGTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGT 6 0.15 No Hit CTGTATAATAATCTAGAGTCTCAATGAACCTTGGAAAGAATGGTTTGGTG 6 0.15 No Hit CCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGCTA 6 0.15 No Hit CTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGA 6 0.15 No Hit AATTGAAGCTAGAGAAGCAACCTGCAGAACGACCTCTAGTGCATTGAAAC 6 0.15 No Hit CCTGTTATTGCCTCAAACTTCCTTGGCCTGGAAGGCCATAGTCCCTCTAA 6 0.15 No Hit GTATTAAGCCTTGACCGTATCACCGACCGAATCACCGTACACCTTCTCGC 5 0.125 No Hit GGCATTTTCACCTGTTGTTATTTTTGTGCAGAATGCAACTCCAGGTTGAT 5 0.125 No Hit GGCATCTCAACACTCCTGACTGCCTTCTCCAACTCCACCATGTCTGTCTC 5 0.125 No Hit ACATGCTGCATTCCGGAGGTAATGCAAGGACGTCTTCTTTCTAGGACATG 5 0.125 No Hit GCAATAAATATCAATCTGAAGTAAGGAGGAAAGGCTAAGATAAGCTTTGA 5 0.125 No Hit GATAAATACTTGGGATTTTGCATAATAAAGAAAAACAAACTTGTTAGATT 5 0.125 No Hit CTCAGTGATCCTCTTCTCATATGGTGCAAAGCCAGCAACTTCCCTGATCA 5 0.125 No Hit CAACAACTTAAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGC 5 0.125 No Hit AAGAAATATCTGTTGTTGGTGATATAGGAAAGGTACTTATGCCTTCTTGT 5 0.125 No Hit CCCGATAGAACTCGCACCGAGCTCCAGCTATCCTGAGGGAAACTTCGGAG 5 0.125 No Hit TGGAGATGGTGGACTCCTGCTGCCATAAACAGCCGGTGGAGAAGGATATG 5 0.125 No Hit GGGCCATTGTGTCTCTATGTCATCATCTACATTAACCTTCCTCCCCAAAC 5 0.125 No Hit GCGCCCGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC 5 0.125 No Hit AGTGAACTTTGCAACATCTTCCTCATCAATGGCAGCAGCAATATCCGCTA 5 0.125 No Hit GGCATGCAATAGATTGATATCCAACCACGAGCATGCATCTTGAACCCGGT 5 0.125 No Hit CTTCTTCCTAAAAGAGCACCCCTCTGTAAGCTTGGCACGACCACCAGTTG 5 0.125 No Hit GCTTTACTTCCACGTAGCCTGAAAGCAGCACTGTCATAAGCTTTTGCAGC 5 0.125 No Hit GTATCTTGCTGGGTATGTGTGTCCCAGTGCTCCCCACAGCTGCTCTTCCC 5 0.125 No Hit ACCAGTTGGACCCAAGAATAGGAATGAGCCAGTCGGCTGTTGAGGCCTGC 5 0.125 No Hit TCTGGGTCCAGCTTCTTGCAGTTGTCTCATACTTGTTCCTGTCGGTCTTG 5 0.125 No Hit GCTCAATGGCTGTGATGCTGTGCCTGCGAGATTGTGTGGATGAGGCTGGG 5 0.125 No Hit TTTTTTTTTTGGATTTTTTCCGCTTTGATATTCTCTGCATCCTATTTAGG 5 0.125 No Hit CCCAGTTGATGAATCCACCGTCGCCGTCGAGGGTGAATACTCCTGATTTA 5 0.125 No Hit CAGAGACTTATCAACAACTCCCGATCCATGGAACTTGACTGTTCCTTTTC 5 0.125 No Hit CCCCCACAAAGAATTGCAAGTCCTTGATTTTTGAAAGCAAGAACTTGGTT 5 0.125 No Hit GCCTCTCGTTCCCATGCTGGTGCACAGTGTTGGAAAACATCTTTCACCCA 5 0.125 No Hit GCACGGTGGGACCTTTAGCACTCTCCTTTGCGGTTCCCATCTGACCATCT 5 0.125 No Hit CTGCCTATGGAGAATTGATTCAAGATTTGCTTCAGTGGTTTACAGCCTCA 5 0.125 No Hit CTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTA 5 0.125 No Hit CCGACCTCCTTGCAAAGCCACAATCTTGTCAGTTGGTACAGTATCTTGCA 5 0.125 No Hit GGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCTTTG 5 0.125 No Hit CCTATTGCCACATAAACTGGCACAAAGTCACCTCTCACCTTCTTGCCATG 5 0.125 No Hit AATGAAAACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAA 5 0.125 No Hit GACATATAATTTATTCTAGTACAGTAGGGCATCCTTTTTTATCATACAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.0625 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.125 0.0 0.0 0.0 0.0 84-85 0.1375 0.0 0.0 0.0 0.0 86-87 0.15 0.0 0.0 0.0 0.0 88-89 0.21250000000000002 0.0 0.0 0.0 0.0 90-91 0.2625 0.0 0.0 0.0 0.0 92-93 0.275 0.0 0.0 0.0 0.0 94-95 0.3 0.0 0.0 0.0 0.0 96-97 0.3875 0.0 0.0 0.0 0.0 98-99 0.4875 0.0 0.0 0.0 0.0 100-101 0.55 0.0 0.0 0.0 0.0 102-103 0.5625 0.0 0.0 0.0 0.0 104-105 0.6 0.0 0.0 0.0 0.0 106-107 0.6499999999999999 0.0 0.0 0.0 0.0 108-109 0.725 0.0 0.0 0.0 0.0 110-111 0.8625 0.0 0.0 0.0 0.0 112-113 0.9625 0.0 0.0 0.0 0.0 114-115 1.125 0.0 0.0 0.0 0.0 116-117 1.3375 0.0 0.0 0.0 0.0 118-119 1.5625 0.0 0.0 0.0 0.0 120-121 1.8125 0.0 0.0 0.0 0.0 122-123 2.05 0.0 0.0 0.0 0.0 124-125 2.325 0.0 0.0 0.0 0.0 126-127 2.7249999999999996 0.0 0.0 0.0 0.0 128-129 3.1125 0.0 0.0 0.0 0.0 130-131 3.4625 0.0 0.0 0.0 0.0 132-133 3.9875 0.0 0.0 0.0 0.0 134-135 4.5625 0.0 0.0 0.0 0.0 136-137 5.175000000000001 0.0 0.0 0.0 0.0 138-139 6.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR26075337 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075337_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.26275 37.0 37.0 37.0 37.0 37.0 2 36.1455 37.0 37.0 37.0 37.0 37.0 3 36.162 37.0 37.0 37.0 37.0 37.0 4 36.33 37.0 37.0 37.0 37.0 37.0 5 36.233 37.0 37.0 37.0 37.0 37.0 6 36.1625 37.0 37.0 37.0 37.0 37.0 7 36.218 37.0 37.0 37.0 37.0 37.0 8 36.278 37.0 37.0 37.0 37.0 37.0 9 36.3835 37.0 37.0 37.0 37.0 37.0 10-14 36.2178 37.0 37.0 37.0 37.0 37.0 15-19 36.1878 37.0 37.0 37.0 37.0 37.0 20-24 36.1116 37.0 37.0 37.0 37.0 37.0 25-29 36.073299999999996 37.0 37.0 37.0 37.0 37.0 30-34 35.932100000000005 37.0 37.0 37.0 37.0 37.0 35-39 35.9112 37.0 37.0 37.0 37.0 37.0 40-44 35.9087 37.0 37.0 37.0 37.0 37.0 45-49 35.8264 37.0 37.0 37.0 37.0 37.0 50-54 35.6931 37.0 37.0 37.0 37.0 37.0 55-59 35.7367 37.0 37.0 37.0 37.0 37.0 60-64 35.765699999999995 37.0 37.0 37.0 37.0 37.0 65-69 35.7019 37.0 37.0 37.0 37.0 37.0 70-74 35.6271 37.0 37.0 37.0 37.0 37.0 75-79 35.585300000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.604699999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.6114 37.0 37.0 37.0 37.0 37.0 90-94 35.4841 37.0 37.0 37.0 37.0 37.0 95-99 35.565000000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.413799999999995 37.0 37.0 37.0 37.0 37.0 105-109 35.35 37.0 37.0 37.0 34.6 37.0 110-114 35.3098 37.0 37.0 37.0 34.6 37.0 115-119 35.332100000000004 37.0 37.0 37.0 32.2 37.0 120-124 35.21445 37.0 37.0 37.0 29.8 37.0 125-129 35.2623 37.0 37.0 37.0 34.6 37.0 130-134 35.1663 37.0 37.0 37.0 29.8 37.0 135-139 34.9803 37.0 37.0 37.0 25.0 37.0 140-144 35.13895 37.0 37.0 37.0 27.4 37.0 145-149 34.97664999999999 37.0 37.0 37.0 25.0 37.0 150-151 34.744875 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 5.0 14 6.0 15 5.0 16 4.0 17 4.0 18 2.0 19 4.0 20 6.0 21 5.0 22 3.0 23 9.0 24 10.0 25 11.0 26 20.0 27 18.0 28 17.0 29 17.0 30 37.0 31 46.0 32 60.0 33 83.0 34 189.0 35 639.0 36 2597.0 37 201.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.73743435858965 21.080270067516878 9.227306826706677 19.954988747186796 2 29.5 23.275000000000002 26.5 20.724999999999998 3 22.075 27.55 31.025000000000002 19.35 4 27.125 33.125 20.275000000000002 19.475 5 27.450000000000003 35.625 19.400000000000002 17.525 6 23.425 35.55 22.525000000000002 18.5 7 23.549999999999997 20.025000000000002 35.4 21.025 8 22.925 25.124999999999996 24.675 27.275 9 26.450000000000003 24.349999999999998 24.7 24.5 10-14 24.95 28.235 24.98 21.834999999999997 15-19 24.945 27.884999999999998 25.575 21.595 20-24 24.955 26.93 26.705000000000002 21.41 25-29 26.125 26.150000000000002 26.08 21.645 30-34 24.895 28.12 25.595000000000002 21.39 35-39 24.635 27.689999999999998 26.009999999999998 21.665 40-44 25.665 27.355 25.275 21.705 45-49 24.895 26.924999999999997 26.57 21.61 50-54 24.779999999999998 27.169999999999998 26.44 21.61 55-59 24.365000000000002 27.46 26.08 22.095000000000002 60-64 25.885 27.07 26.05 20.995 65-69 25.355 27.72 25.995 20.93 70-74 25.0 27.060000000000002 27.295 20.645 75-79 25.224999999999998 27.529999999999998 26.22 21.025 80-84 25.28 27.744999999999997 25.81 21.165 85-89 26.185000000000002 27.065 25.180000000000003 21.57 90-94 25.055 26.369999999999997 26.565 22.009999999999998 95-99 25.06 27.994999999999997 26.855 20.09 100-104 25.415 27.54 26.484999999999996 20.560000000000002 105-109 25.8 28.549999999999997 25.724999999999998 19.925 110-114 25.555 27.77 25.64 21.035 115-119 25.61 27.005000000000003 26.845000000000002 20.54 120-124 25.65128256412821 27.496374818740936 25.896294814740738 20.95604780239012 125-129 25.490000000000002 27.6 25.745 21.165 130-134 25.314999999999998 27.825 25.7 21.16 135-139 25.982598259825984 28.467846784678468 24.497449744974496 21.052105210521052 140-144 26.513977096564485 27.734160124018604 25.298794819222888 20.45306796019403 145-149 26.451612903225808 27.326831707926978 25.456364091022753 20.765191297824455 150-151 27.65345668208526 27.57844730591324 25.14064258032254 19.62745343167896 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.5 11 1.0 12 0.0 13 0.5 14 1.0 15 0.5 16 0.0 17 0.0 18 1.0 19 1.5 20 0.5 21 1.5 22 2.5 23 1.5 24 3.0 25 4.0 26 3.0 27 3.0 28 5.0 29 8.5 30 9.5 31 13.5 32 21.5 33 21.0 34 24.5 35 50.0 36 68.5 37 89.5 38 119.0 39 143.0 40 163.0 41 177.0 42 191.5 43 197.0 44 223.0 45 252.5 46 243.0 47 224.5 48 237.5 49 213.5 50 169.5 51 151.0 52 116.5 53 104.0 54 101.0 55 90.0 56 76.0 57 64.0 58 58.0 59 60.5 60 59.0 61 48.0 62 32.5 63 22.5 64 24.0 65 12.5 66 9.0 67 10.5 68 5.5 69 5.0 70 3.0 71 3.5 72 3.5 73 0.5 74 0.0 75 0.0 76 0.5 77 0.5 78 0.0 79 0.5 80 1.0 81 0.5 82 2.5 83 3.0 84 2.0 85 1.5 86 0.5 87 1.5 88 1.5 89 1.0 90 0.5 91 1.0 92 1.5 93 1.0 94 1.0 95 1.5 96 1.0 97 0.0 98 0.5 99 1.0 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.005 125-129 0.0 130-134 0.0 135-139 0.01 140-144 0.015 145-149 0.025 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.199999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 64.17483660130719 39.275 2 21.241830065359476 26.0 3 8.006535947712418 14.7 4 3.553921568627451 8.7 5 1.6339869281045754 5.0 6 0.7352941176470588 2.7 7 0.28594771241830064 1.225 8 0.24509803921568626 1.2 9 0.04084967320261438 0.22499999999999998 >10 0.08169934640522876 0.975 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 28 0.7000000000000001 No Hit GTCGGGGGCATTCGTATTTCATAGTCAGAGGTGAAATTCTTGGATTTATG 11 0.27499999999999997 No Hit GTGAATTTCAGGCAGAAGTTGAGATCATTAGCCGTGTGCATCACAAACAT 9 0.22499999999999998 No Hit GCCATTTCGTGCCCTACTTGATGTTGGGTTAGTAAGAACTACAACTGGAA 8 0.2 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 8 0.2 No Hit TCTTGATCAAATATTACAAAGAAGCAAGACGGGCTCTTTGCTTAAGCAAT 8 0.2 No Hit AAGAGAGGGCACGTCAGTGCTGGCCATGGACGTATCGGCAAGCACAGGAA 8 0.2 No Hit CATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCGCCCGTCG 8 0.2 No Hit GTTGGGGGCTCGAAGACGATCAGATACCGTCCTAGTCTCAACCATAAACG 8 0.2 No Hit GAATATCCCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATTG 7 0.17500000000000002 No Hit GTTAGTTACAAGTTTAAGGATTTTGATGCTGCTGTTGAAGACCTTTCTGC 7 0.17500000000000002 No Hit GTGCAATTCAAGGAAGAAAATGATTGAGTTGTTGCTAAAAGGCGAAAGTT 7 0.17500000000000002 No Hit GTTGTTGGTTTATGCTCATCCCTACTCATAACCCCAGCACTTAGATATTT 7 0.17500000000000002 No Hit GACACGCTGAGCGGAACGCTTTCGTCCGTGGATGTTGCCACGAAAGAGAA 7 0.17500000000000002 No Hit GTGTCAAGCAAGGGGAGGCATGGTGGGAGGGAAAACATGGAGAGCGGTGG 7 0.17500000000000002 No Hit GCGGTACAGGCCATACTAGGGAACATGTATTCTGTTAGTAGTGGCCAAGT 7 0.17500000000000002 No Hit GCCAAAAGCTTCCTGTCATCATGCATAAGACTCAAAAGCTTGTGGTCCAC 6 0.15 No Hit GGTGTTACTAAGCCTGGTACCAGCAAATGTTCCACTGTTGGTATTCAAGG 6 0.15 No Hit ATTACACGAAAGATTTTGATGTTGGCCATATCCCATTGAGGTTGCCAAGA 6 0.15 No Hit GTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGGAAGCTGACTGG 6 0.15 No Hit CTCCATCTCCATGGCTTCAATTTCTATGTTGTTGGAGAAGGGTTTGGGAA 6 0.15 No Hit TGCATCAAGTGCTCCACCACCTGGTGGTGCACCTGTGGTCCGACCACCTG 6 0.15 No Hit GGCCGCTTCGGCGGCCGATCCGGGCGGAAGACATTGTCAGGTGGGGAGTT 6 0.15 No Hit ACCAACGTGACAACTCAAGTTCGCGGGACAATGGGCCACATAGCACCAGA 6 0.15 No Hit GATGTTGCCATGGATGGTTGTGAGGTTCAACTGGAACATCTCAGGGTCCA 6 0.15 No Hit AGCGAACCGGGAAATGCCCAGCTTGAGAATCTGGCGCCTGCGGCGTCCGA 6 0.15 No Hit GCAAGCCATGAGGATACATTCAAGTCTCTGGAATGCTGATGATTGGGCAA 6 0.15 No Hit CCTCATGTCATCTGTCTGGATGAGCCTACTAACTACCTCGATCGTGAATC 6 0.15 No Hit GCTGAGGACGATGACGATGCGGAAAATGATGGGTCTGCGGAGGACACCAT 6 0.15 No Hit GACAAAGCATGATCAGGACCTTCCATATTTTTATTTAAGGTAAAGTGCTT 6 0.15 No Hit GCTGAGGAATCTGCTCTGGGCAACATCGAAACATGATGTGTATCTTATGC 6 0.15 No Hit GGAGCGAAAGATAATGGATATTGGCCTTGCAAACTGGCAAAAGGCATGCT 6 0.15 No Hit CCTTTGGAGTGTTAAAATTGTTGATCTAAGCTACCTCTTCCGTTATCCTC 6 0.15 No Hit TCTCACTCGTCTTCTCAACAGTCAGAAATCTTGAGCACAAAACCCTAATC 6 0.15 No Hit CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 5 0.125 No Hit GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTCTATAGCC 5 0.125 No Hit CAGCTCCAATAGCGTATATTTAAGTTGTTGCAGTTAAAAAGCTCGTAGTT 5 0.125 No Hit CTTGCATAGAAACTTGATTCTCACTCAAAGCACTGGACTGCTCATGTCTC 5 0.125 No Hit CAAGCCTACGCTCTGGATACATTAGCATGGGATAACATCATAGGATTTCG 5 0.125 No Hit GTTCGAGTGAGAGCATGCCTGTCGGGACCCGAAAGATGGTGAACTATGCC 5 0.125 No Hit GCTGATACGTACTAAACGCAAAGTATATATTTGCAATGGCTGTCGTGGAA 5 0.125 No Hit GGGGAATCCGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCCTGCGG 5 0.125 No Hit GCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCC 5 0.125 No Hit CAGAAATATCTGGAGTGTACTTTTTTGGTGGAAAGGGCAGGACTCTGAAC 5 0.125 No Hit GGATCCAATTTCTCACATTTTCCTTGCCGCATTTATCACTTGAACACCGT 5 0.125 No Hit GATTTAGCCAGAGTTGCTGATTTGCGCTATGGGGCGCTGGAGGAAGTGGA 5 0.125 No Hit GGCGATTCTTTTCTTCATAGTATGGGGATTTATTGACGGACTATTCTGGG 5 0.125 No Hit GGCAGGAGAGGTTGAAATGGGTCCGGGAGAATTACATGGTATACAATTAC 5 0.125 No Hit ACAAGAGCCATGAGTATGAGACCAAAGTTGGTGAAGAGAGTGGTGCTGTT 5 0.125 No Hit GGAGAAGGCCAAGACAGAGAAGAAAAGGCCAGCTGCGGTTCCAGCCAACA 5 0.125 No Hit GCAAAGCCAAGAATCTTGAAATATTTACCAAATCTTTGTGTTTCCCTCTT 5 0.125 No Hit AAGGCAGATGTTTCAAGTGAACAAGAAAGGCATTACAGGGGCGTTAGGCG 5 0.125 No Hit GCAAAGCCCAAAGCTGAACCTGCTGTGGAAGAGGAAGAGGCACCAAAGCC 5 0.125 No Hit AAAACAGCCAAATACCGGTCTCTTTGTGGGTTTGAACAAGGGGCACGTAG 5 0.125 No Hit GCCATCCAAAGGACCTATGTGGAGAGGAGTCATAGTTGCCTATATAGTGG 5 0.125 No Hit GAAACGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCAAT 5 0.125 No Hit GGTCACTGCTTATGTCGCAGAAGAGTTTAGAAAAGCGTTTTGGTCAGTCA 5 0.125 No Hit GCCAGAGGGAATACCAGAGAGTGCATCAGTTCAGGCAATATTAGACTGGC 5 0.125 No Hit GTCGAGTTAAATGACAATCTTGGCTTAGCCATAATGTTCATCCATGACTT 5 0.125 No Hit GTTCAATCCTTTGAACAATATACCTAAAGACAGTGAATTGTATGTGGCAA 5 0.125 No Hit GTTGTTGCAGTTAAAAAGCTCGTAGTTGGACTTTGGGTTGGGTCGGCCGG 5 0.125 No Hit GAAATATAGCATGCCAAAACCAGCTTCCAGCTGATGACTGTACCTGCGAA 5 0.125 No Hit GCAAAACAGCTGCTGCTGCTCCCGTTGAAGCTGCCCCAGCCAAGGAGGCT 5 0.125 No Hit CGCGTGCCGGCCGGGGGACGGGCTGGGAACGGCCCCTTCGGGGGCCTTCC 5 0.125 No Hit TAGATAAAAGGTCGACGCGGGCTCTGCCCGTTGCTCTGATGATTCATGAT 5 0.125 No Hit TGATGACCAAGCTGCCAAGGTTGTTGACATCGTTGACACATTTAGGCTCC 5 0.125 No Hit GATAAAACGGTTCTTGCTCATGAAGATCATTGTGTTTGTGTTTTGTGTGG 5 0.125 No Hit AAAAGAACAGCCGAACCACCGTGATCGGCCGTCCTGGTGAGCCAATTTGG 5 0.125 No Hit GGATGGGCGCCCGCGGCGTTGACTGGGAGCACTGGCACTACGAGCTCGAT 5 0.125 No Hit GGAAGAGACTCGCAAGAAGGTTTGGCTTGTAGACTCAAAGGTAGCAGTGC 5 0.125 No Hit CGCGAGTCATCAGCTCGCGTTGACTACGTCCCTGCCCTTTGTACACACCG 5 0.125 No Hit GCTCTTCGTGGTGTTCCAAGGTGTTTCAAATGTAGCTGGTGAAACAAGTT 5 0.125 No Hit TGTCTTTTCTGCTTATTGTTATAGTAACAAAATTCATCGATGCTGATAAC 5 0.125 No Hit GGCAGATCGTCTTGTTAAGATTGGGCAGGAGGGTTTTGCAGCCATTGATG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.0625 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.125 0.0 0.0 0.0 0.0 84-85 0.1375 0.0 0.0 0.0 0.0 86-87 0.15 0.0 0.0 0.0 0.0 88-89 0.21250000000000002 0.0 0.0 0.0 0.0 90-91 0.2625 0.0 0.0 0.0 0.0 92-93 0.275 0.0 0.0 0.0 0.0 94-95 0.3 0.0 0.0 0.0 0.0 96-97 0.3875 0.0 0.0 0.0 0.0 98-99 0.5 0.0 0.0 0.0 0.0 100-101 0.575 0.0 0.0 0.0 0.0 102-103 0.5874999999999999 0.0 0.0 0.0 0.0 104-105 0.625 0.0 0.0 0.0 0.0 106-107 0.675 0.0 0.0 0.0 0.0 108-109 0.75 0.0 0.0 0.0 0.0 110-111 0.9125 0.0 0.0 0.0 0.0 112-113 1.0125 0.0 0.0 0.0 0.0 114-115 1.225 0.0 0.0 0.0 0.0 116-117 1.4375 0.0 0.0 0.0 0.0 118-119 1.6625 0.0 0.0 0.0 0.0 120-121 1.9125 0.0 0.0 0.0 0.0 122-123 2.1500000000000004 0.0 0.0 0.0 0.0 124-125 2.425 0.0 0.0 0.0 0.0 126-127 2.8499999999999996 0.0 0.0 0.0 0.0 128-129 3.25 0.0 0.0 0.0 0.0 130-131 3.6125 0.0 0.0 0.0 0.0 132-133 4.1375 0.0 0.0 0.0 0.0 134-135 4.7375 0.0 0.0 0.0 0.0 136-137 5.3375 0.0 0.0 0.0 0.0 138-139 6.125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTGGTC 10 0.006830828 145.0 145 >>END_MODULE Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132928 spots for SRR26075337.sra Written 2132928 spots for SRR26075337.sra Read 2132931 spots for SRR26075337.sra Written 2132931 spots for SRR26075337.sra SRR ids: ['SRR26075337.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_a09l8qeq SRR26075337.sra spots: 42658563 blocks: [[1, 2132928], [2132929, 4265856], [4265857, 6398784], [6398785, 8531712], [8531713, 10664640], [10664641, 12797568], [12797569, 14930496], [14930497, 17063424], [17063425, 19196352], [19196353, 21329280], [21329281, 23462208], [23462209, 25595136], [25595137, 27728064], [27728065, 29860992], [29860993, 31993920], [31993921, 34126848], [34126849, 36259776], [36259777, 38392704], [38392705, 40525632], [40525633, 42658563]] SRR26075337 file size 15755543 SRR26075337 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075337 SRR26075337_1.fastq SRR26075337_2.fastq Input file: SRR26075337_1.fastq Paired file: SRR26075337_2.fastq trimmed: SRR26075337-trimmed-pair1.fastq, SRR26075337-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 21:21:29 2025 >> started Tue Feb 11 21:22:20 2025 >> done (51.068s) 42658563 read pairs processed; of these: 197 ( 0.00%) short read pairs filtered out after trimming by size control 56563 ( 0.13%) empty read pairs filtered out after trimming by size control 42601803 (99.87%) read pairs available; of these: 5136590 (12.06%) trimmed read pairs available after processing 37465213 (87.94%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 14 0.00% 19 13 0.00% 20 21 0.00% 21 27 0.00% 22 29 0.00% 23 35 0.00% 24 56 0.00% 25 48 0.00% 26 69 0.00% 27 65 0.00% 28 97 0.00% 29 84 0.00% 30 118 0.00% 31 102 0.00% 32 115 0.00% 33 82 0.00% 34 132 0.00% 35 156 0.00% 36 159 0.00% 37 164 0.00% 38 188 0.00% 39 202 0.00% 40 239 0.00% 41 201 0.00% 42 236 0.00% 43 247 0.00% 44 243 0.00% 45 294 0.00% 46 298 0.00% 47 276 0.00% 48 359 0.00% 49 361 0.00% 50 425 0.00% 51 451 0.00% 52 412 0.00% 53 479 0.00% 54 467 0.00% 55 574 0.00% 56 566 0.00% 57 651 0.00% 58 723 0.00% 59 736 0.00% 60 751 0.00% 61 796 0.00% 62 886 0.00% 63 964 0.00% 64 934 0.00% 65 967 0.00% 66 1129 0.00% 67 1143 0.00% 68 1336 0.00% 69 1347 0.00% 70 1407 0.00% 71 1593 0.00% 72 1846 0.00% 73 1934 0.00% 74 2047 0.00% 75 2129 0.00% 76 2242 0.01% 77 2320 0.01% 78 2589 0.01% 79 2943 0.01% 80 3167 0.01% 81 3327 0.01% 82 3668 0.01% 83 4008 0.01% 84 4435 0.01% 85 4711 0.01% 86 5212 0.01% 87 5359 0.01% 88 5557 0.01% 89 5932 0.01% 90 6445 0.02% 91 7188 0.02% 92 8838 0.02% 93 9447 0.02% 94 9920 0.02% 95 11238 0.03% 96 11512 0.03% 97 11864 0.03% 98 13161 0.03% 99 13772 0.03% 100 15016 0.04% 101 16480 0.04% 102 17636 0.04% 103 19491 0.05% 104 21134 0.05% 105 22518 0.05% 106 23688 0.06% 107 25385 0.06% 108 27177 0.06% 109 29009 0.07% 110 30666 0.07% 111 34056 0.08% 112 37164 0.09% 113 39799 0.09% 114 43588 0.10% 115 46345 0.11% 116 50185 0.12% 117 52199 0.12% 118 54082 0.13% 119 57044 0.13% 120 59598 0.14% 121 63161 0.15% 122 68342 0.16% 123 73790 0.17% 124 80646 0.19% 125 86710 0.20% 126 90618 0.21% 127 93409 0.22% 128 97752 0.23% 129 103599 0.24% 130 106876 0.25% 131 109065 0.26% 132 118419 0.28% 133 124121 0.29% 134 133624 0.31% 135 139067 0.33% 136 145502 0.34% 137 151143 0.35% 138 154475 0.36% 139 159138 0.37% 140 160554 0.38% 141 167407 0.39% 142 174296 0.41% 143 183420 0.43% 144 190888 0.45% 145 196959 0.46% 146 199852 0.47% 147 210221 0.49% 148 210014 0.49% 149 216758 0.51% 150 214226 0.50% 151 37465213 87.94% 42601803 reads passed initial QC criterion=sequence-density sequence-density=0.16 sequence-density-rank=1 fanout-score=3.22 fanout-score-rank=25 prefix-density=0.22 prefix-fanout=2.3 sequence=CACTTGCAGCCATTCTCAGCACCA criterion=fanout-score sequence-density=0.03 sequence-density-rank=36 fanout-score=61.73 fanout-score-rank=1 prefix-density=0.99 prefix-fanout=2.1 sequence=CGCATCGCCGGCCCCCATCCACTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCAGACAGCGCCTCGTGGTGCGGCAGGGTCCAGCCACGACGGGGCTCTCACCCTCTCCGGCGCCCCTTTCCAGGGGACTTGGGCCTGGTCCGCCGCTGAGGACGCTTCTCCAGACTACAATTCGGACGCCGCAGGCGCCAGATTCTCAAGCTGGGCATTTCCCGGTTCGCTCGCCGTTACTAGGGGAATCCTTGTAAGTTTCTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTAGTCCCGCCTGAC criterion=sequence-density sequence-density=0.19 sequence-density-rank=1 fanout-score=2.93 fanout-score-rank=34 prefix-density=0.19 prefix-fanout=2.9 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.06 sequence-density-rank=33 fanout-score=16.64 fanout-score-rank=1 prefix-density=0.15 prefix-fanout=6.3 sequence=GAAAACAATGGCACTACATGGAAAGATTGAGACAACATTAGAACTCAAGTCCTCCGCAGAGAAGTTCTACAAAGTGTGGAGGAGCCAGTCCTTCCATGTTCCCAAACATGCTTCCAAGCATATCCAAGGAGTTGATATACATGCAGGTGACTGGGAGACTGCGGGCTCTATCAGGATTTGGCAGTACACAATCGGAGGGAAAGCCGGGGTCTTTAAAGAGGAGGTTTCC SRR26075337 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 21:23:14 Started mapping on | Feb 11 21:23:14 Finished on | Feb 11 21:38:15 Mapping speed, Million of reads per hour | 170.22 Number of input reads | 42601803 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 28972278 Uniquely mapped reads % | 68.01% Average mapped length | 296.92 Number of splices: Total | 24219712 Number of splices: Annotated (sjdb) | 23598086 Number of splices: GT/AG | 23789403 Number of splices: GC/AG | 316282 Number of splices: AT/AC | 26602 Number of splices: Non-canonical | 87425 Mismatch rate per base, % | 0.44% Deletion rate per base | 0.03% Deletion average length | 2.98 Insertion rate per base | 0.02% Insertion average length | 2.63 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1120051 % of reads mapped to multiple loci | 2.63% Number of reads mapped to too many loci | 4763656 % of reads mapped to too many loci | 11.18% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 15.41% % of reads unmapped: other | 2.77% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 12509474 12509474 12509474 N_multimapping 1120051 1120051 1120051 N_noFeature 975677 28598144 1178661 N_ambiguous 380730 7017 203985 UnstrandedReadsAssigned:27615871 PositiveStrandReadsAssigned:367117 NegativeStrandReadsAssigned:27589632 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075337 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075337-trimmed-pair1.fastq SRR26075337-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 42,601,803 reads, 31,495,249 reads pseudoaligned [quant] estimated average fragment length: 214.522 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,260 rounds 52401 SRR26075337.ke.tsv 34699 SRR26075337.se.tsv 87100 total ==> SRR26075337.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1804.48 6257 90.8529 Potri.005G024800.1.v4.1 1035 821.478 4755 151.663 Potri.004G059700.1.v4.1 961 747.49 38 1.332 Potri.007G009000.2.v4.1 1416 1202.48 0 0 Potri.003G141000.2.v4.1 2943 2729.48 1276.85 12.257 Potri.016G087400.1.v4.1 270 83.6788 2636.75 825.614 Potri.015G069301.1.v4.1 564 351.981 0 0 Potri.010G195200.1.v4.1 1773 1559.48 122 2.04977 Potri.012G127500.1.v4.1 977 763.49 4448 152.646 ==> SRR26075337.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 282 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 377 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 662 SRR26075337 completed mapping pipeline successfully