Starting /dee2/code/volunteer_pipeline.sh SRR26075338
    current disk space = 3052953075712
    free memory = 1414575548 
SRR26075338 SRAfilesize
1971ccaf17fce7144954fa51503fa311  SRR26075338.sra
SRR26075338.sra file validated
SRR26075338 is paired end
SRR26075338 is conventional basespace
SRR26075338 read1 length is 151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26075338_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	151
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	36.48	37.0	37.0	37.0	37.0	37.0
2	36.5075	37.0	37.0	37.0	37.0	37.0
3	36.5795	37.0	37.0	37.0	37.0	37.0
4	36.6385	37.0	37.0	37.0	37.0	37.0
5	36.6275	37.0	37.0	37.0	37.0	37.0
6	36.652	37.0	37.0	37.0	37.0	37.0
7	36.6035	37.0	37.0	37.0	37.0	37.0
8	36.634	37.0	37.0	37.0	37.0	37.0
9	36.645	37.0	37.0	37.0	37.0	37.0
10-14	36.613	37.0	37.0	37.0	37.0	37.0
15-19	36.565200000000004	37.0	37.0	37.0	37.0	37.0
20-24	36.523900000000005	37.0	37.0	37.0	37.0	37.0
25-29	36.5519	37.0	37.0	37.0	37.0	37.0
30-34	36.4234	37.0	37.0	37.0	37.0	37.0
35-39	36.411899999999996	37.0	37.0	37.0	37.0	37.0
40-44	36.3224	37.0	37.0	37.0	37.0	37.0
45-49	36.31400000000001	37.0	37.0	37.0	37.0	37.0
50-54	36.26460000000001	37.0	37.0	37.0	37.0	37.0
55-59	36.2197	37.0	37.0	37.0	37.0	37.0
60-64	36.1914	37.0	37.0	37.0	37.0	37.0
65-69	36.0868	37.0	37.0	37.0	37.0	37.0
70-74	36.1207	37.0	37.0	37.0	37.0	37.0
75-79	36.0343	37.0	37.0	37.0	37.0	37.0
80-84	36.083600000000004	37.0	37.0	37.0	37.0	37.0
85-89	35.9686	37.0	37.0	37.0	37.0	37.0
90-94	35.9353	37.0	37.0	37.0	37.0	37.0
95-99	35.907	37.0	37.0	37.0	37.0	37.0
100-104	35.8917	37.0	37.0	37.0	37.0	37.0
105-109	35.8022	37.0	37.0	37.0	37.0	37.0
110-114	35.6648	37.0	37.0	37.0	37.0	37.0
115-119	35.5655	37.0	37.0	37.0	37.0	37.0
120-124	35.6164	37.0	37.0	37.0	37.0	37.0
125-129	35.407	37.0	37.0	37.0	37.0	37.0
130-134	35.3798	37.0	37.0	37.0	34.6	37.0
135-139	35.241	37.0	37.0	37.0	29.8	37.0
140-144	35.229499999999994	37.0	37.0	37.0	29.8	37.0
145-149	35.1105	37.0	37.0	37.0	25.0	37.0
150-151	35.0355	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150-151	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	3.0
22	3.0
23	2.0
24	4.0
25	5.0
26	6.0
27	16.0
28	14.0
29	18.0
30	48.0
31	40.0
32	55.0
33	94.0
34	147.0
35	475.0
36	2891.0
37	177.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	47.33668341708543	12.964824120603016	6.130653266331659	33.5678391959799
2	19.2	13.8	32.7	34.300000000000004
3	15.325	19.325	32.35	33.0
4	23.1	26.875	24.25	25.775
5	24.075	33.050000000000004	23.275000000000002	19.6
6	22.5	33.45	23.974999999999998	20.075000000000003
7	16.625	25.724999999999998	42.05	15.6
8	17.299999999999997	26.6	30.075000000000003	26.025
9	17.575	23.1	33.575	25.75
10-14	20.18	28.115000000000002	27.900000000000002	23.805
15-19	21.029999999999998	26.435	28.244999999999997	24.29
20-24	19.925	27.26	28.525	24.29
25-29	20.65	28.215	27.694999999999997	23.44
30-34	20.135	28.625	27.66	23.580000000000002
35-39	19.68	27.605	27.865000000000002	24.85
40-44	20.59	27.48	27.150000000000002	24.779999999999998
45-49	19.895	26.955000000000002	28.98	24.169999999999998
50-54	20.200000000000003	28.035	27.915	23.849999999999998
55-59	21.245	27.26	27.794999999999998	23.7
60-64	20.72	26.924999999999997	28.71	23.645
65-69	20.585	28.395	27.279999999999998	23.74
70-74	20.68	28.27	27.82	23.23
75-79	21.36	27.505000000000003	27.445000000000004	23.69
80-84	21.495	27.68	27.515	23.31
85-89	21.725	27.725	27.92	22.63
90-94	21.2	28.325	27.18	23.294999999999998
95-99	20.995	27.93	27.18	23.895
100-104	20.74	27.955000000000002	27.229999999999997	24.075
105-109	20.585	27.04	27.815	24.560000000000002
110-114	21.68	27.950000000000003	27.065	23.305
115-119	22.245	27.54	27.13	23.085
120-124	21.51	27.72	26.83	23.94
125-129	22.189999999999998	27.46	26.584999999999997	23.765
130-134	21.029999999999998	27.41	27.265	24.295
135-139	21.195	27.779999999999998	26.97	24.055
140-144	21.099999999999998	27.46	27.655	23.785
145-149	22.285	27.99	25.869999999999997	23.855
150-151	20.849999999999998	27.5875	27.737499999999997	23.825
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.5
18	0.5
19	0.5
20	1.5
21	1.0
22	0.0
23	1.5
24	2.0
25	2.0
26	3.5
27	2.0
28	2.5
29	8.0
30	11.5
31	13.0
32	19.0
33	25.0
34	30.5
35	55.0
36	82.5
37	87.5
38	115.5
39	166.0
40	187.0
41	199.5
42	241.0
43	272.0
44	277.0
45	272.0
46	270.0
47	262.5
48	249.0
49	229.0
50	190.0
51	166.0
52	136.5
53	97.0
54	74.0
55	52.0
56	44.5
57	36.0
58	19.0
59	16.0
60	16.5
61	15.5
62	13.0
63	7.5
64	3.0
65	4.0
66	3.0
67	4.0
68	5.0
69	3.5
70	2.0
71	1.5
72	0.5
73	0.0
74	0.0
75	0.0
76	0.0
77	0.0
78	0.0
79	0.0
80	0.0
81	0.0
82	0.0
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.5
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.9
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.37802907915994	39.85
2	21.365105008077546	26.450000000000003
3	7.996768982229402	14.85
4	3.5541195476575123	8.799999999999999
5	1.2924071082390953	4.0
6	0.6865912762520193	2.55
7	0.32310177705977383	1.4000000000000001
8	0.32310177705977383	1.6
9	0.04038772213247173	0.22499999999999998
>10	0.04038772213247173	0.27499999999999997
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGCATCCTGGTTACCATCACCATATCTAGAGCTCCAACTGCTTTTCTTGT	11	0.27499999999999997	No Hit
ACCTGCTGCATCTCCTCTCATTGAACATGATGAACACCATATCACTTGCT	9	0.22499999999999998	No Hit
GCATATTATGTCCCCATCTTCGATCTGACTTATTCGAAATGAGGCCCTAT	8	0.2	No Hit
CCACAATCTTCCCAACCAAATTACTGACCTTAACCTGGTCACCATTGTTG	8	0.2	No Hit
CCTGCCACCACTTCTGTTGCTGGTTTCATTGATGGAGAGTTCGCGATTAA	8	0.2	No Hit
GTCGCATGGGAGGAGACATGTGGTGAGGAGTAGACGGAGACCTTTGATAC	8	0.2	No Hit
TCAGGTCTGAAGGAGTACCTCATCTTCCGGACAATACAATCCCAAACAGA	8	0.2	No Hit
GAATACGAACAACATGTGGCCACTTGACAAATCTAGTCAAATCCTTCTTT	8	0.2	No Hit
ATCATGGCCATTTCCATTAGTTTCCTCATTAAGCATATGATCTCCTTTCA	8	0.2	No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCTTGTTATCTCGTAT	8	0.2	TruSeq Adapter, Index 11 (97% over 37bp)
GGGAAGAAACGTTTGTAGTAGAGAGTGTTGGAGAGCAGTCGAGCCATGGC	7	0.17500000000000002	No Hit
ACATGTTCTTCCCCATCTCCATCTTAAAGTAACCGTTGTCGCCCCAGTCT	7	0.17500000000000002	No Hit
GTACTGGCCTCCCCCATGGCCACAGCATGCTTTGGTACCATATTTGAGCC	7	0.17500000000000002	No Hit
GGCAAGGAATAGTGATGGGGCCTTCTTGATCAAATCCAAACTCTTCCTCA	7	0.17500000000000002	No Hit
GTCAGGATATCTGCAGCCCACCATACAAAGTTTTCCCGTTTCAGCATCAT	7	0.17500000000000002	No Hit
GTTCTTGTATGATGACTGATTAATGAAATCGTGTGTGCTTGGTTTTTGTA	7	0.17500000000000002	No Hit
GACAAATCCAGTGTATCCTGCCATTACACCGTGAATTGCCGAGTATGCTA	7	0.17500000000000002	No Hit
CCGCAATATGTCAATCTTCTCCTCCTCACCTACTTCATGCAGCACAGAAC	7	0.17500000000000002	No Hit
CTTTATGAGATTGCTTAATCTTTTACATTTAGCATCCATTTGGGGTTTTT	6	0.15	No Hit
CCTCCATTGTGGTTCTTCATCCTCCAAGTCAATGGTGTAGGCCTCGCCTG	6	0.15	No Hit
CAACTAACTGATTTCCCATCAACTGAAAAAGTTACCACCCTCTTCTTCTT	6	0.15	No Hit
CCTGTAGGATCGGATTGGTGGCCAGCCCACTATTTGTACCTTCGGAGCAG	6	0.15	No Hit
GTGTAAATGGGTTTGCCAGATTCAAGAAGTAAAGTGACATGGTTGTCCAT	6	0.15	No Hit
GGGGTTTCCATCAACAAGTGAATTCTCATATATCTCTTTGCACTCTTGCA	6	0.15	No Hit
CGTACTTCCTCTTGCTTCTTCCCACCAGATTTGAGATACCATCCCAGAGC	6	0.15	No Hit
CTTAGTTCCATCAGGCCTGATCAAAGTATTCACTTTCTTGGTCTGAATGT	6	0.15	No Hit
GGGGAGTCTTGGTCGGGCAAAGGGAGAAGGAAGCTGGGTTTCTCTCAGGT	6	0.15	No Hit
GCGTAAGTATCATTGTTGATGCTTGGAAATTCAGTTTTCCCATTTGATAT	6	0.15	No Hit
GGGCAATGATACCAGCAGCAGCGACAAGAGAATCAGTGAGAGTTGATTTT	6	0.15	No Hit
CTTGTTGAATATCTGCCCTCTCTCCATGCTTTTAGGTCCCGCAACCTGAG	6	0.15	No Hit
GTTTGCAATGTTGTTTGAGAGCCAGTCCAATCCCTCATACAGCCCCTCAC	6	0.15	No Hit
GATCGCTGTCACTTGAGATGAAGATCACCTCAAATGCATTGTCTTTTGCT	6	0.15	No Hit
GTTAATTTCCTTCTTGATGGCCTCAACATAATCTTCCTCTGAGACCTTCT	6	0.15	No Hit
CTCCCTTTCATGGCTCTTGCCATTGCTGTACTCACTCTTATATGAACCTT	6	0.15	No Hit
CCAGATGACTGCTACCAGCTTTGAGATAGTGTGGTGACCTCTCAGGTCCT	6	0.15	No Hit
ATCTCCTCTTCCTCCTTTTTCTTGTGCTCTTCCTCTACTTTAGTCTCGGG	5	0.125	No Hit
GTTGGAGCGGGAGGTGGAGTTGTAGCAGGGACTGATGGAGCGGGAGTTGG	5	0.125	No Hit
GTTTGGTTCAGTCCATGGTAAAAGAGAGCCATGTTTCCGTAACTGTGGGC	5	0.125	No Hit
GTTGGAAAGAGAACGTATTGAGCAAATTGAACAGCTTGCTCCCTGAATTC	5	0.125	No Hit
ACTCCTTTACACTGGGTGCGGTTAGAAACTCAAGTTCCTCATACTGCTGC	5	0.125	No Hit
AGGCAGTCTTGTTCTCCAAGAAATTATCCCAAGCCTTCCCGCTCAAGATA	5	0.125	No Hit
GTCAGGGTACATGCCACATTTGCAGCCACTGCCACACTTGCAGTCAGAGC	5	0.125	No Hit
GCCCAATATATCAACACCAGAGAGCAGTTGAAGAGGTTGGCGAAGTAGAT	5	0.125	No Hit
CCACTTTGTTCGGCTTCCATGGGAGTAGTTCTGGCTTTCACTTGGCCTGG	5	0.125	No Hit
CTTATCTTCCTCCTCAGCGACCACATCCTCCTCCTCCTGCTCTTTTGCAC	5	0.125	No Hit
CAACAACAAATTTTCTGGCTTAAGGTCTCTGTGAAAAACACCCCTGCTGT	5	0.125	No Hit
GTTCAGGTTGCTGAGAATTAGACTTTCCAGCTTCAAGAGGGAAATTCAAG	5	0.125	No Hit
GCCAATCTCCCAACAGCCAGAACCAAAACAGAAGCTATACCACTATTAGC	5	0.125	No Hit
GCAGGAAATGAGGGGTGTTGAGCCAAGGCAAGACCAAGGTAGGCCGATCG	5	0.125	No Hit
GCGGAATACCCGTAACCTGGGTCTTGTTGTCAGTCCCTGTGGAGAAAACC	5	0.125	No Hit
AATCGCTCAGGAGGAACATCAATTTCACCGTATGACTTCAACAAGTCATG	5	0.125	No Hit
CCCAAGCATTCTGACAGACCATTCTCCAGAGATCCTCATTCTTTGTCAGC	5	0.125	No Hit
GCTCCGAGATTGGAATTGAGACGGAGTTCCGAATCTCTTCCCGGGTTGAG	5	0.125	No Hit
GTACCTTCAGGGAGCTCGGGCTGGTCAAATGGGGAACAAAGACTCTTGGC	5	0.125	No Hit
GGGAAATCAAGAGTCTGATCTGGTAGCTGTGAATGATGAAAGAAGACCAG	5	0.125	No Hit
GCCTGAGAGGCTGATCTGACATGGTTCTTGGCATGTTGGTTCAGGGATCT	5	0.125	No Hit
AGACAGGACAAGAATATCAGCACAGGAAACGACTCCAGGACACTCTCTTT	5	0.125	No Hit
CTGAGATGTTCTTGAGAAGCACAACTATGAATTCTGAAGTTAAGTACAAT	5	0.125	No Hit
GGTGACGTGGGTTCTGTAGAGCCTCAACTAAGAAAGGATCCATCATTGAC	5	0.125	No Hit
AACTTGTCTTCTATAGCATCTGCTCCTCATGATACAGATACAATCCAAGC	5	0.125	No Hit
GTCGAAGAGGTCCAGCTGTAGGCCGGGGAAAGATTTTGACAGCCTTCTTC	5	0.125	No Hit
GGCAGCAAATCCACCAGCAATGTCAGTCACTGCAGTAAAACCAGCAGCTA	5	0.125	No Hit
GCGGAGGAGGAGGAGGAGGCGGTACCTGAGCCAAACGATGATGGATCTGA	5	0.125	No Hit
GGGTGATATCGATCACTTGGTAATGATATAGACGGCATAAATGATTCCAG	5	0.125	No Hit
GGGGACATATCCAGAGAGTCTCCAGTAGATAATTTCCCAGCCTTTAGGTT	5	0.125	No Hit
GCTGACTCCATTATCGCAGGAAACCTTACCATTAGTATCAAAAATGACAA	5	0.125	No Hit
CTCCGCCAAGCAATCCCACCAAAATGTATATCCATCGCTTGCGTTTCTTT	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.0	0.0	0.0	0.0	0.0
54-55	0.0	0.0	0.0	0.0	0.0
56-57	0.0	0.0	0.0	0.0	0.0
58-59	0.0	0.0	0.0	0.0	0.0
60-61	0.0	0.0	0.0	0.0	0.0
62-63	0.0	0.0	0.0	0.0	0.0
64-65	0.0	0.0	0.0	0.0	0.0
66-67	0.0	0.0	0.0	0.0	0.0
68-69	0.0	0.0	0.0	0.0	0.0
70-71	0.0	0.0	0.0	0.0	0.0
72-73	0.0	0.0	0.0	0.0	0.0
74-75	0.0	0.0	0.0	0.0	0.0
76-77	0.025	0.0	0.0	0.0	0.0
78-79	0.025	0.0	0.0	0.0	0.0
80-81	0.025	0.0	0.0	0.0	0.0
82-83	0.037500000000000006	0.0	0.0	0.0	0.0
84-85	0.05	0.0	0.0	0.0	0.0
86-87	0.05	0.0	0.0	0.0	0.0
88-89	0.1	0.0	0.0	0.0	0.0
90-91	0.125	0.0	0.0	0.0	0.0
92-93	0.125	0.0	0.0	0.0	0.0
94-95	0.175	0.0	0.0	0.0	0.0
96-97	0.3	0.0	0.0	0.0	0.0
98-99	0.375	0.0	0.0	0.0	0.0
100-101	0.425	0.0	0.0	0.0	0.0
102-103	0.44999999999999996	0.0	0.0	0.0	0.0
104-105	0.475	0.0	0.0	0.0	0.0
106-107	0.5375	0.0	0.0	0.0	0.0
108-109	0.65	0.0	0.0	0.0	0.0
110-111	0.8500000000000001	0.0	0.0	0.0	0.0
112-113	0.9875	0.0	0.0	0.0	0.0
114-115	1.1	0.0	0.0	0.0	0.0
116-117	1.275	0.0	0.0	0.0	0.0
118-119	1.5875	0.0	0.0	0.0	0.0
120-121	2.0625	0.0	0.0	0.0	0.0
122-123	2.3625	0.0	0.0	0.0	0.0
124-125	2.6375	0.0	0.0	0.0	0.0
126-127	3.0875	0.0	0.0	0.0	0.0
128-129	3.7125000000000004	0.0	0.0	0.0	0.0
130-131	4.0875	0.0	0.0	0.0	0.0
132-133	4.525	0.0	0.0	0.0	0.0
134-135	4.925	0.0	0.0	0.0	0.0
136-137	5.5875	0.0	0.0	0.0	0.0
138-139	6.137499999999999	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATGTT	10	0.006830828	145.0	6
TCAGGTC	10	0.006830828	145.0	1
CCCATTC	10	0.006830828	145.0	145
CTGAAGG	10	0.006830828	145.0	7
TCTGAAG	10	0.006830828	145.0	6
CAGGTCT	10	0.006830828	145.0	2
>>END_MODULE
SRR26075338 read2 length is 151 nt
##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR26075338_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4000
Sequences flagged as poor quality	0
Sequence length	151
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	35.532	37.0	37.0	37.0	37.0	37.0
2	36.1355	37.0	37.0	37.0	37.0	37.0
3	36.141	37.0	37.0	37.0	37.0	37.0
4	36.067	37.0	37.0	37.0	37.0	37.0
5	36.117	37.0	37.0	37.0	37.0	37.0
6	36.08	37.0	37.0	37.0	37.0	37.0
7	36.1855	37.0	37.0	37.0	37.0	37.0
8	36.11	37.0	37.0	37.0	37.0	37.0
9	36.2	37.0	37.0	37.0	37.0	37.0
10-14	36.1062	37.0	37.0	37.0	37.0	37.0
15-19	35.99980000000001	37.0	37.0	37.0	37.0	37.0
20-24	35.9769	37.0	37.0	37.0	37.0	37.0
25-29	35.8239	37.0	37.0	37.0	37.0	37.0
30-34	35.7542	37.0	37.0	37.0	37.0	37.0
35-39	35.70920000000001	37.0	37.0	37.0	37.0	37.0
40-44	35.665	37.0	37.0	37.0	37.0	37.0
45-49	35.712599999999995	37.0	37.0	37.0	37.0	37.0
50-54	35.4945	37.0	37.0	37.0	37.0	37.0
55-59	35.5257	37.0	37.0	37.0	37.0	37.0
60-64	35.5637	37.0	37.0	37.0	37.0	37.0
65-69	35.504200000000004	37.0	37.0	37.0	37.0	37.0
70-74	35.477900000000005	37.0	37.0	37.0	37.0	37.0
75-79	35.395500000000006	37.0	37.0	37.0	37.0	37.0
80-84	35.425	37.0	37.0	37.0	37.0	37.0
85-89	35.3876	37.0	37.0	37.0	37.0	37.0
90-94	35.29090000000001	37.0	37.0	37.0	37.0	37.0
95-99	35.291	37.0	37.0	37.0	34.6	37.0
100-104	35.2879	37.0	37.0	37.0	34.6	37.0
105-109	35.2064	37.0	37.0	37.0	32.2	37.0
110-114	35.1399	37.0	37.0	37.0	29.8	37.0
115-119	35.24209999999999	37.0	37.0	37.0	32.2	37.0
120-124	35.0552	37.0	37.0	37.0	27.4	37.0
125-129	35.0429	37.0	37.0	37.0	25.0	37.0
130-134	34.9748	37.0	37.0	37.0	25.0	37.0
135-139	34.7962	37.0	37.0	37.0	25.0	37.0
140-144	34.8055	37.0	37.0	37.0	25.0	37.0
145-149	34.773	37.0	37.0	37.0	25.0	37.0
150-151	34.36125	37.0	37.0	37.0	25.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.0
1101	2	0.0
1101	3	0.0
1101	4	0.0
1101	5	0.0
1101	6	0.0
1101	7	0.0
1101	8	0.0
1101	9	0.0
1101	10-14	0.0
1101	15-19	0.0
1101	20-24	0.0
1101	25-29	0.0
1101	30-34	0.0
1101	35-39	0.0
1101	40-44	0.0
1101	45-49	0.0
1101	50-54	0.0
1101	55-59	0.0
1101	60-64	0.0
1101	65-69	0.0
1101	70-74	0.0
1101	75-79	0.0
1101	80-84	0.0
1101	85-89	0.0
1101	90-94	0.0
1101	95-99	0.0
1101	100-104	0.0
1101	105-109	0.0
1101	110-114	0.0
1101	115-119	0.0
1101	120-124	0.0
1101	125-129	0.0
1101	130-134	0.0
1101	135-139	0.0
1101	140-144	0.0
1101	145-149	0.0
1101	150-151	0.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	4.0
14	5.0
15	10.0
16	10.0
17	7.0
18	2.0
19	7.0
20	3.0
21	6.0
22	11.0
23	15.0
24	17.0
25	18.0
26	17.0
27	15.0
28	15.0
29	22.0
30	35.0
31	44.0
32	59.0
33	113.0
34	220.0
35	733.0
36	2433.0
37	179.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.425000000000004	20.95	10.05	19.575
2	29.875	24.25	27.075	18.8
3	22.650000000000002	27.325	31.424999999999997	18.6
4	22.2	33.125	24.05	20.625
5	24.85	37.6	19.5	18.05
6	23.974999999999998	37.225	20.075000000000003	18.725
7	21.875	23.05	36.199999999999996	18.875
8	23.400000000000002	26.3	26.55	23.75
9	23.925	28.050000000000004	24.925	23.1
10-14	24.47	29.485	25.435000000000002	20.61
15-19	24.25	28.185	26.834999999999997	20.73
20-24	24.224999999999998	28.57	27.22	19.985
25-29	24.445	28.375	26.915	20.265
30-34	24.834999999999997	28.660000000000004	26.72	19.785
35-39	24.095	29.465000000000003	25.915	20.525
40-44	25.005	28.494999999999997	26.38	20.119999999999997
45-49	24.415	26.919999999999998	26.939999999999998	21.725
50-54	23.465	28.575	27.54	20.419999999999998
55-59	23.525	28.15	26.955000000000002	21.37
60-64	24.36	28.83	26.484999999999996	20.325
65-69	24.59	28.71	27.11	19.59
70-74	24.45	29.17	25.895000000000003	20.485
75-79	24.2	28.125	26.93	20.745
80-84	24.18	28.005000000000003	27.49	20.325
85-89	24.375	27.474999999999998	27.905	20.244999999999997
90-94	24.605	28.09	26.36	20.945
95-99	24.275	28.860000000000003	26.07	20.794999999999998
100-104	24.779999999999998	27.744999999999997	26.57	20.905
105-109	25.415	27.060000000000002	27.395000000000003	20.13
110-114	24.349999999999998	27.435	27.650000000000002	20.565
115-119	23.505000000000003	29.099999999999998	26.979999999999997	20.415
120-124	24.88	28.335	26.229999999999997	20.555
125-129	25.019999999999996	28.199999999999996	26.235000000000003	20.544999999999998
130-134	24.785	28.535	26.255	20.424999999999997
135-139	25.369999999999997	28.249999999999996	25.955000000000002	20.424999999999997
140-144	25.005	28.465	26.650000000000002	19.88
145-149	26.595000000000002	28.134999999999998	25.895000000000003	19.375
150-151	26.325	28.0875	26.6625	18.925
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	0.5
7	1.0
8	2.0
9	2.0
10	1.0
11	1.5
12	1.5
13	0.5
14	1.5
15	1.0
16	1.5
17	1.5
18	0.5
19	0.5
20	1.0
21	1.0
22	0.5
23	1.5
24	1.5
25	2.0
26	2.0
27	3.5
28	7.5
29	8.0
30	6.5
31	8.0
32	13.0
33	26.0
34	40.0
35	48.5
36	60.5
37	83.5
38	120.0
39	160.5
40	182.0
41	222.5
42	252.0
43	251.0
44	276.5
45	298.0
46	286.0
47	269.5
48	237.0
49	186.5
50	187.0
51	175.0
52	128.0
53	88.5
54	61.0
55	50.0
56	50.5
57	55.5
58	32.0
59	10.0
60	11.5
61	10.0
62	4.0
63	4.5
64	5.5
65	4.5
66	3.5
67	3.0
68	3.0
69	2.0
70	3.0
71	2.0
72	1.0
73	1.0
74	1.0
75	2.5
76	3.0
77	2.0
78	0.5
79	0.5
80	1.0
81	1.0
82	1.5
83	1.0
84	0.0
85	0.0
86	0.5
87	1.5
88	1.5
89	0.5
90	0.5
91	0.5
92	0.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.5
99	0.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-151	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
151	4000.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.73267326732673	42.125
2	19.92079207920792	25.15
3	7.247524752475247	13.725000000000001
4	3.2871287128712874	8.3
5	1.3465346534653466	4.25
6	0.7524752475247525	2.85
7	0.31683168316831684	1.4000000000000001
8	0.31683168316831684	1.6
9	0.0	0.0
>10	0.07920792079207921	0.6
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	13	0.325	No Hit
GGACAGTGAGATAGATGGAAACCGGAATTCTAGCTGGGGTAAGAAAAGCA	11	0.27499999999999997	No Hit
GTTCTGGCAAGCCAATAGAAATTCTAGCAAAGCTCAATCAAATGGCTGGC	8	0.2	No Hit
GTGCAAGTAACTTAGAAGGAACCATCCCAGTTTGTCCCAATAAATAGCGT	8	0.2	No Hit
CACAAGACAACCGCAAAGACAGAAAAAACAAAGAGCCAACTTTCAAACAA	8	0.2	No Hit
CTTCACCCTCACATGATAGGTCACCATCACCTGTTCGACGTCGTAGGTCA	8	0.2	No Hit
CATAGAAGGTTCTAAGCTGGTTGTCTCAGGCGGATGTACAGATGCCGGGG	8	0.2	No Hit
CTGGAGTGAGATTGTTGCTTTTGCAGTTTTGATTAGCTTTGTGGTTTTAT	8	0.2	No Hit
TATTTCCAACTTGCTGCTGAAGACCATGAGTGGTGGTGGAGGTCTTTCCT	8	0.2	No Hit
ATCAATTCCAGCATGGGCAGTAGGGCCACCATTGATCTTGGTGGGAGTTT	8	0.2	No Hit
CTTTAATCCTCTACAAGCCCTAATACCTCCCTCTCTCTCTGTCACACCAT	7	0.17500000000000002	No Hit
GGAGATAAAAATCATTTGAGTTTCCATATTCACTTGCCAAATCACCATCA	7	0.17500000000000002	No Hit
GACTATAATAACATGTTGAAAAAGGCTCTAAGCCAAACTAGAATGGAACT	7	0.17500000000000002	No Hit
GGGCTCCTTACTCTCCGGTTAAAGGAAAAACCAACCACAGCATCCCACTG	7	0.17500000000000002	No Hit
GGTCAACCCATCTGACCCGTGACCCGATCACTTGACCGGGTCGATGACCG	7	0.17500000000000002	No Hit
CAAACACGTTCAGGTTTTCGATCTGCAAGTCTCCTCTTCTAGATGGCTCC	7	0.17500000000000002	No Hit
GCGTGGTTAAAGTCAGAGCTTAAGAACTGGTGGACAAGAGACCATGCAAA	7	0.17500000000000002	No Hit
GTCAACATTACACTGGGTGCTGAAGACGAATTGAAGCATGCAGTTGCATT	7	0.17500000000000002	No Hit
CCACAGGAGCCTCGATCCAGACCTTGTTAAATGAGCAGCGCGTTTGGATG	6	0.15	No Hit
GCTAAGCACACAAATTAAGGCTTAAATATATAGAGAGAAAGAAACAACAT	6	0.15	No Hit
GAGCAAGAAAAACTGGAGAAACTTGCACGCAAGGGTCCTAAACCCGAGGA	6	0.15	No Hit
GCTTGAGCAAATTCAGTTTCTAAGCAAAAGCTTTCCAGGCCCCTTTATCC	6	0.15	No Hit
GGACATTGAAGAAGGAGAGGAATCCCAAGTATCCACGCATCAGTGCAACA	6	0.15	No Hit
GTGCATACAACATTTTCCTCCTTGTATATATTCCTTTGTGACTTCTTTTC	6	0.15	No Hit
GACAAGAAAAAAAAGAAAACCCTAAACCTCTACTTTTCACAAGGGAGCCG	6	0.15	No Hit
GCAGAAACAATAACTCAGGTTTCTGACAGTGTAATTCCAGCATTCAAATC	6	0.15	No Hit
GGCCAGGAAAGGAGTACGAGGATAAGCTCAAAGATTGTTGACGAAGAAGG	6	0.15	No Hit
AGATTGTTGGAAAACATGGAAGGTGGTGTTGCATATGAGAATGATCTAAA	6	0.15	No Hit
GGCTAGATGAAGAGGTTATTATGGACTTCTTCAGGGAGTATATCACAGTG	6	0.15	No Hit
CATTACTTCCAGAACACACAAGGACTTATCTTTGTGGTTGATAGCAATGA	6	0.15	No Hit
GCTCTTAATGCTGAGCTTGAAAAAGACGTGGAGCGTGTCAGACAAAGGGA	6	0.15	No Hit
AATGGGGAAAATGATTTTGTGATTGGCAAAAGTGGCTCCAAGGTCCCAGT	6	0.15	No Hit
ATGGTGATCAGGTCAAGGTCAGCGATTTGGTTGGGAAGATTGTGGGATTC	6	0.15	No Hit
CAAATGCAGCAGCTCAGGCTTCTAGAAAATCCTCACCACGGGTCACCAAC	6	0.15	No Hit
TCACTTGGAGGTAAATAAAGGCTCACGCAACAAGAAGTTCGTTGATAGAA	6	0.15	No Hit
GAGTGAGTGACCCTAAAAAACTACTCTCCTATTCACTCTTTCGCCGCCTC	6	0.15	No Hit
GCTGCATCGGATGTTGAATGAGGATGAATTGAGGGATGCTGTGCTACTTG	6	0.15	No Hit
CTCTAAATTTCGCTATGAAATTCATCAAAAAACTCAGCCAGAAAATCTTG	5	0.125	No Hit
CTCTTCCTTAAACGGGCCTTTGGCGGTTCTCTACAAATTTTTGGATTACT	5	0.125	No Hit
TGACTGTGCTGTTCAGTCATGTGATGCTTCACTGCTGCTGGACTCAACAA	5	0.125	No Hit
GTTTCAATGGCCATGTCACCTAGTCAAAGGCCAAGAGGAGTACTCGGGTT	5	0.125	No Hit
GGAAGTGTGACTGTGGTATACCGTGTCACTATACGAGGATCTGATGGAGA	5	0.125	No Hit
ACCAGCTTGAGCAAATTCAGTTTCTAAGCAAAAGCTTTCCAGGCCCCTTT	5	0.125	No Hit
GTAAGCAGCTGTCATTATTTAATTAGCCGTCTGTGAAGAGGTCAAGTTTC	5	0.125	No Hit
GCCCAGGAAGTGGGGTGGTATTAGGTCTTTCCATTTGAAGTTGCTTGATA	5	0.125	No Hit
TTCATATTGTGATTTTGATCAGTATGGCTGAGGAAAACAAGAGCCATGAG	5	0.125	No Hit
GGGTTGGCTTATCTCTCTCTCTAAAATCTATCAATGAAACCCTTTGTTTT	5	0.125	No Hit
AAATCTTTAGAGGGTCTCAAAAACCTATCTTTCTTCGAATCAAGAATGAG	5	0.125	No Hit
ATTAATGGATGCTGCCTTTGTCATTATGTTATTGCTGAAGTGGACTTACA	5	0.125	No Hit
CTTGACTAATGATGTGAGTCCAAAGTGGGCTCCTTCTAATTTCAGCCAAA	5	0.125	No Hit
AGACAGTCTTTCCTTCCTTAAGCTGGAATTGTACAGTTAAGCAGCCAAAG	5	0.125	No Hit
GAGCAATTAACATTCCTTACATGTACAGAGTTGGATCAGGAATGACCAAG	5	0.125	No Hit
GACAATTTGGCGGAGAAAGTAAAGGATATGCTGGATGATATTCAAAAGAG	5	0.125	No Hit
GTGTTACTTTCTAACTTGTATGCTGGATTAGGGAAATGGGATGGTGTGCG	5	0.125	No Hit
CCGCTATCCAAGCATTACAGCCAGACTTAATTATTAATGCAGGCACTGCT	5	0.125	No Hit
TTCCTCTTCTATTTGCCTTCACTGAGTTTAGGCCTCTTGATCGCCACATT	5	0.125	No Hit
GCTTGATGCTTTTGGCTCTCTTAGCTGGTTCAGCCTTGGCTCAGGCACCA	5	0.125	No Hit
GACTGGTCCAATGCATCGTGAGGTTGCTAACTGTTGTCGGTACCTGGCCA	5	0.125	No Hit
GTTGAAAATGGCTGAAGAATCAAAACCGGGGTTGAGGAAGCCTGTATTCA	5	0.125	No Hit
GATCAAGGCAGATGTTTCAAGTGAACAAGAAAGGCATTACAGGGGCGTTA	5	0.125	No Hit
GAGCAATTCGCTAGCCTGTTAAAGTTAATTAATTAGCAGTTAGTTAGTTA	5	0.125	No Hit
ATCCCAATGAGATGAATAAGAAAACCACTTCCTACAAATTTAATGTTCTT	5	0.125	No Hit
GATTGAGACAATGAAGCTTATCAAGCATCCAAATGTTGTTCGATTATATG	5	0.125	No Hit
ACTGTACTTCCATTGATTTTTCGGGTAATTATTTCCAAGGAAAGGTTACT	5	0.125	No Hit
TGTTGGTTCTATATTACAGTAACATCGAAAATGAGTTCAGCAAGCAAAGC	5	0.125	No Hit
CAGGTTTGGAGAGAGCACTTTAAAATTGGGATCTATGTTAACGGATGGGC	5	0.125	No Hit
GGTGATCGTAATGTTAGCCGTGGCATCTGTGGGATACTGCAATTGAGTGA	5	0.125	No Hit
GGATGGGCTGGAGTAATCTGGCTATTTAGCTTGGTGACATATCTGCCGCT	5	0.125	No Hit
GGAACTCAACAAAACTACCTCTCGAACCAGAACATTAATCAGCTCGGTGG	5	0.125	No Hit
AATTGATAGCCGCCCTTCTCTCGCTAACAGGTAAGACATGGTTAAGCATA	5	0.125	No Hit
TTCAGGGTGTTCTTCTCTTGAAAACGATGTGCTGAGATTGTCTTGTTTCG	5	0.125	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	0.0	0.0
22-23	0.0	0.0	0.0	0.0	0.0
24-25	0.0	0.0	0.0	0.0	0.0
26-27	0.0	0.0	0.0	0.0	0.0
28-29	0.0	0.0	0.0	0.0	0.0
30-31	0.0	0.0	0.0	0.0	0.0
32-33	0.0	0.0	0.0	0.0	0.0
34-35	0.0	0.0	0.0	0.0	0.0
36-37	0.0	0.0	0.0	0.0	0.0
38-39	0.0	0.0	0.0	0.0	0.0
40-41	0.0	0.0	0.0	0.0	0.0
42-43	0.0	0.0	0.0	0.0	0.0
44-45	0.0	0.0	0.0	0.0	0.0
46-47	0.0	0.0	0.0	0.0	0.0
48-49	0.0	0.0	0.0	0.0	0.0
50-51	0.0	0.0	0.0	0.0	0.0
52-53	0.025	0.0	0.0	0.0	0.0
54-55	0.025	0.0	0.0	0.0	0.0
56-57	0.025	0.0	0.0	0.0	0.0
58-59	0.025	0.0	0.0	0.0	0.0
60-61	0.025	0.0	0.0	0.0	0.0
62-63	0.025	0.0	0.0	0.0	0.0
64-65	0.025	0.0	0.0	0.0	0.0
66-67	0.025	0.0	0.0	0.0	0.0
68-69	0.025	0.0	0.0	0.0	0.0
70-71	0.025	0.0	0.0	0.0	0.0
72-73	0.025	0.0	0.0	0.0	0.0
74-75	0.025	0.0	0.0	0.0	0.0
76-77	0.05	0.0	0.0	0.0	0.0
78-79	0.05	0.0	0.0	0.0	0.0
80-81	0.05	0.0	0.0	0.0	0.0
82-83	0.0625	0.0	0.0	0.0	0.0
84-85	0.075	0.0	0.0	0.0	0.0
86-87	0.075	0.0	0.0	0.0	0.0
88-89	0.125	0.0	0.0	0.0	0.0
90-91	0.15	0.0	0.0	0.0	0.0
92-93	0.15	0.0	0.0	0.0	0.0
94-95	0.225	0.0	0.0	0.0	0.0
96-97	0.35	0.0	0.0	0.0	0.0
98-99	0.425	0.0	0.0	0.0	0.0
100-101	0.475	0.0	0.0	0.0	0.0
102-103	0.5	0.0	0.0	0.0	0.0
104-105	0.525	0.0	0.0	0.0	0.0
106-107	0.5875	0.0	0.0	0.0	0.0
108-109	0.75	0.0	0.0	0.0	0.0
110-111	1.0	0.0	0.0	0.0	0.0
112-113	1.1375	0.0	0.0	0.0	0.0
114-115	1.25	0.0	0.0	0.0	0.0
116-117	1.425	0.0	0.0	0.0	0.0
118-119	1.7375	0.0	0.0	0.0	0.0
120-121	2.2125000000000004	0.0	0.0	0.0	0.0
122-123	2.5375	0.0	0.0	0.0	0.0
124-125	2.8125	0.0	0.0	0.0	0.0
126-127	3.2625	0.0	0.0	0.0	0.0
128-129	3.875	0.0	0.0	0.0	0.0
130-131	4.237500000000001	0.0	0.0	0.0	0.0
132-133	4.675000000000001	0.0	0.0	0.0	0.0
134-135	5.05	0.0	0.0	0.0	0.0
136-137	5.7125	0.0	0.0	0.0	0.0
138-139	6.275	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCAGAG	10	0.006830828	145.0	1
CAGGATC	10	0.006830828	145.0	145
TACTTCC	10	0.006830828	145.0	4
AGATTGT	10	0.006830828	145.0	9
TTAGATT	10	0.006830828	145.0	2
TTTTTTT	40	0.0076550315	18.125	5
>>END_MODULE
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106806 spots for SRR26075338.sra
Written 3106806 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
Read 3106793 spots for SRR26075338.sra
Written 3106793 spots for SRR26075338.sra
SRR ids: ['SRR26075338.sra']
extra args: ['--split-files', '--defline-qual', '+']
tempdir: /tmp/pfd_bmub9gg1
SRR26075338.sra spots: 62135873
blocks: [[1, 3106793], [3106794, 6213586], [6213587, 9320379], [9320380, 12427172], [12427173, 15533965], [15533966, 18640758], [18640759, 21747551], [21747552, 24854344], [24854345, 27961137], [27961138, 31067930], [31067931, 34174723], [34174724, 37281516], [37281517, 40388309], [40388310, 43495102], [43495103, 46601895], [46601896, 49708688], [49708689, 52815481], [52815482, 55922274], [55922275, 59029067], [59029068, 62135873]]
SRR26075338 file size 22954252
SRR26075338 completed basic pipeline successfully
skewer v0.2.2 [April 4, 2016]
COMMAND LINE:	skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075338 SRR26075338_1.fastq SRR26075338_2.fastq
Input file:	SRR26075338_1.fastq
Paired file:	SRR26075338_2.fastq
trimmed:	SRR26075338-trimmed-pair1.fastq, SRR26075338-trimmed-pair2.fastq

Parameters used:
-- 3' end adapter sequence (-x):	AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC
-- paired 3' end adapter sequence (-y):	AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA
-- maximum error ratio allowed (-r):	0.100
-- maximum indel error ratio allowed (-d):	0.030
-- end quality threshold (-q):		10
-- minimum read length allowed after trimming (-l):	18
-- file format (-f):		Sanger/Illumina 1.8+ FASTQ 
-- number of concurrent threads (-t):	20
Tue Feb 11 20:47:43 2025 >> started

Tue Feb 11 20:49:00 2025 >> done (76.828s)
62135873 read pairs processed; of these:
     344 ( 0.00%) short read pairs filtered out after trimming by size control
  147690 ( 0.24%) empty read pairs filtered out after trimming by size control
61987839 (99.76%) read pairs available; of these:
 6564942 (10.59%) trimmed read pairs available after processing
55422897 (89.41%) untrimmed read pairs available after processing

Length distribution of reads after trimming:
length	count	percentage
 18	      26	  0.00%
 19	      20	  0.00%
 20	      46	  0.00%
 21	      58	  0.00%
 22	      44	  0.00%
 23	      58	  0.00%
 24	      57	  0.00%
 25	      66	  0.00%
 26	      78	  0.00%
 27	      56	  0.00%
 28	      76	  0.00%
 29	     102	  0.00%
 30	      88	  0.00%
 31	      89	  0.00%
 32	      94	  0.00%
 33	     100	  0.00%
 34	     107	  0.00%
 35	     136	  0.00%
 36	     115	  0.00%
 37	     136	  0.00%
 38	     172	  0.00%
 39	     168	  0.00%
 40	     144	  0.00%
 41	     149	  0.00%
 42	     182	  0.00%
 43	     152	  0.00%
 44	     233	  0.00%
 45	     191	  0.00%
 46	     246	  0.00%
 47	     257	  0.00%
 48	     298	  0.00%
 49	     325	  0.00%
 50	     338	  0.00%
 51	     350	  0.00%
 52	     348	  0.00%
 53	     356	  0.00%
 54	     424	  0.00%
 55	     513	  0.00%
 56	     517	  0.00%
 57	     487	  0.00%
 58	     540	  0.00%
 59	     587	  0.00%
 60	     700	  0.00%
 61	     854	  0.00%
 62	     866	  0.00%
 63	     905	  0.00%
 64	     900	  0.00%
 65	     956	  0.00%
 66	    1106	  0.00%
 67	    1131	  0.00%
 68	    1200	  0.00%
 69	    1287	  0.00%
 70	    1432	  0.00%
 71	    1687	  0.00%
 72	    1787	  0.00%
 73	    1947	  0.00%
 74	    2000	  0.00%
 75	    2287	  0.00%
 76	    2270	  0.00%
 77	    2527	  0.00%
 78	    2765	  0.00%
 79	    2904	  0.00%
 80	    3373	  0.01%
 81	    3829	  0.01%
 82	    4275	  0.01%
 83	    4468	  0.01%
 84	    4967	  0.01%
 85	    5191	  0.01%
 86	    5464	  0.01%
 87	    5830	  0.01%
 88	    6454	  0.01%
 89	    6757	  0.01%
 90	    7783	  0.01%
 91	    8445	  0.01%
 92	    9419	  0.02%
 93	   10769	  0.02%
 94	   11679	  0.02%
 95	   12489	  0.02%
 96	   13167	  0.02%
 97	   14837	  0.02%
 98	   15319	  0.02%
 99	   16501	  0.03%
100	   18045	  0.03%
101	   19704	  0.03%
102	   22181	  0.04%
103	   24138	  0.04%
104	   26739	  0.04%
105	   28866	  0.05%
106	   31457	  0.05%
107	   32563	  0.05%
108	   34819	  0.06%
109	   37066	  0.06%
110	   39231	  0.06%
111	   43339	  0.07%
112	   46373	  0.07%
113	   50944	  0.08%
114	   55959	  0.09%
115	   60824	  0.10%
116	   64303	  0.10%
117	   67600	  0.11%
118	   72353	  0.12%
119	   75821	  0.12%
120	   79567	  0.13%
121	   85199	  0.14%
122	   91075	  0.15%
123	   99531	  0.16%
124	  105576	  0.17%
125	  112389	  0.18%
126	  119463	  0.19%
127	  124578	  0.20%
128	  128840	  0.21%
129	  134744	  0.22%
130	  138901	  0.22%
131	  144402	  0.23%
132	  152885	  0.25%
133	  161487	  0.26%
134	  170204	  0.27%
135	  180356	  0.29%
136	  186181	  0.30%
137	  192373	  0.31%
138	  199584	  0.32%
139	  202492	  0.33%
140	  210697	  0.34%
141	  215288	  0.35%
142	  222408	  0.36%
143	  229063	  0.37%
144	  241100	  0.39%
145	  250385	  0.40%
146	  256458	  0.41%
147	  261302	  0.42%
148	  264408	  0.43%
149	  267375	  0.43%
150	  273250	  0.44%
151	55422897	 89.41%
61987839 reads passed initial QC


criterion=sequence-density
sequence-density=0.25
sequence-density-rank=1
fanout-score=3.42
fanout-score-rank=25
prefix-density=0.39
prefix-fanout=2.2
sequence=CACTTGCAGCCATTCTCAGCACC


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=34
fanout-score=168.17
fanout-score-rank=1
prefix-density=0.16
prefix-fanout=13.5
sequence=ATTTCATCAAAAAAGGAACGTACATGTGGATGATATACACCCCAGTTTATTTAAATTAGGAGGCCATTTATAACATATAATTTATTCTAGTACAGTAGGGCATCCTTTTTTATCATACAACTCAAAAGACTCACAAGACTCACGATCGAGGACATTCATCATCTCATCACTCACAAGCAAGTCGTGGCGTAGGAATTGAATTAATGAACTTGGTGGCAGCAAGGAAGGCGTTGTAGGAGGCTAAATAGCTAACTCCTTTGTTTGAACTTGTTTCACCAGCTACATTTGAAACACCTTGGAACACCACGAAGAGCTTTTCATTTGACAAAGATGTCAAAGCTACAGAAGCACTTGCTGTATCGGCAGTTTTTGCATCAAAATTATCGTTAAGGAAGTCTCCGTA


criterion=sequence-density
sequence-density=0.33
sequence-density-rank=1
fanout-score=2.99
fanout-score-rank=23
prefix-density=0.34
prefix-fanout=3.0
sequence=ATGTACCCTGACTT


criterion=fanout-score
sequence-density=0.01
sequence-density-rank=31
fanout-score=363.07
fanout-score-rank=1
prefix-density=0.18
prefix-fanout=13.4
sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGGCAAGCTTAGAAAGATCAGCACCAGACCACTTAACATCAAAGATATTGACGTCGGAGAGGGGAGCAGTCCTGTTAATAAGTTTCAGAGGTCCATGACTATGCCAGGAACTCCAGGGACACCGACGACACCAGTGACCCCTACAACCCCAGTGTCGGCGCGTAGCAATGTTTGGAGGAGCGTGTTCCACCCTGGTAGCAACCTTGCTACTAAGAATATTGGTGCTCATGTTTTTGACAAGCCACAGCCTAACACACCCACTGTCTATGACTGGATGTACAGTGGAGAGACGAAGAGCGAGCATCGTTGATGAGGTTGCCTTCAACCAAGGTTGCCCATGTAAATACGTACTGTGTTTTGTTTTTCAGTACTCATCTGCAATATGTCTCTTGTTGTTATGGTTCTACGGTTCTACCGTGCCTGGAA
SRR26075338 testing PE reads STAR mapping to Ensembl genome
                                 Started job on |	Feb 11 20:49:40
                             Started mapping on |	Feb 11 20:49:40
                                    Finished on |	Feb 11 20:57:50
       Mapping speed, Million of reads per hour |	455.42

                          Number of input reads |	61987839
                      Average input read length |	298
                                    UNIQUE READS:
                   Uniquely mapped reads number |	56198961
                        Uniquely mapped reads % |	90.66%
                          Average mapped length |	296.56
                       Number of splices: Total |	53344787
            Number of splices: Annotated (sjdb) |	52096981
                       Number of splices: GT/AG |	52374316
                       Number of splices: GC/AG |	730082
                       Number of splices: AT/AC |	61137
               Number of splices: Non-canonical |	179252
                      Mismatch rate per base, % |	0.42%
                         Deletion rate per base |	0.03%
                        Deletion average length |	3.00
                        Insertion rate per base |	0.02%
                       Insertion average length |	2.62
                             MULTI-MAPPING READS:
        Number of reads mapped to multiple loci |	1586966
             % of reads mapped to multiple loci |	2.56%
        Number of reads mapped to too many loci |	198342
             % of reads mapped to too many loci |	0.32%
                                  UNMAPPED READS:
       % of reads unmapped: too many mismatches |	0.00%
                 % of reads unmapped: too short |	6.13%
                     % of reads unmapped: other |	0.33%
                                  CHIMERIC READS:
                       Number of chimeric reads |	0
                            % of chimeric reads |	0.00%
N_unmapped	4201912	4201912	4201912
N_multimapping	1586966	1586966	1586966
N_noFeature	1407014	55600826	1744642
N_ambiguous	627351	3255	364818
UnstrandedReadsAssigned:54164596 PositiveStrandReadsAssigned:594880 NegativeStrandReadsAssigned:54089501
Dataset is classified negative stranded
MeadianReadLen=151 20thPercentileLength=151 echo kmer=147
SRR26075338 Starting Kallisto paired end mapping to ensembl reference transcriptome

[quant] fragment length distribution will be estimated from the data
[index] k-mer length: 31
[index] number of targets: 52,400
[index] number of k-mers: 62,057,036
[index] number of equivalence classes: 130,681
[quant] running in paired-end mode
[quant] will process pair 1: SRR26075338-trimmed-pair1.fastq
                             SRR26075338-trimmed-pair2.fastq
[quant] finding pseudoalignments for the reads ... done
[quant] processed 61,987,839 reads, 54,747,767 reads pseudoaligned
[quant] estimated average fragment length: 221.264
[   em] quantifying the abundances ... done
[   em] the Expectation-Maximization algorithm ran for 1,226 rounds

  52401 SRR26075338.ke.tsv
  34699 SRR26075338.se.tsv
  87100 total
==> SRR26075338.ke.tsv <==
target_id	length	eff_length	est_counts	tpm
Potri.005G200100.1.v4.1	2018	1797.74	7225	63.4277
Potri.005G024800.1.v4.1	1035	814.736	4007	77.6193
Potri.004G059700.1.v4.1	961	740.741	20	0.426119
Potri.007G009000.2.v4.1	1416	1195.74	0	0
Potri.003G141000.2.v4.1	2943	2722.74	2434.08	14.109
Potri.016G087400.1.v4.1	270	83.5984	5773	1089.86
Potri.015G069301.1.v4.1	564	346.419	0	0
Potri.010G195200.1.v4.1	1773	1552.74	1182	12.014
Potri.012G127500.1.v4.1	977	756.736	40927	853.558

==> SRR26075338.se.tsv <==
Potri.001G166300.v4.1	0
Potri.001G448400.v4.1	668
Potri.001G233950.v4.1	0
Potri.001G122700.v4.1	942
Potri.001G212900.v4.1	0
Potri.001G182400.v4.1	0
Potri.001G256600.v4.1	0
Potri.001G040500.v4.1	0
Potri.001G416900.v4.1	0
Potri.001G452600.v4.1	2052
SRR26075338 completed mapping pipeline successfully