Starting /dee2/code/volunteer_pipeline.sh SRR26075339 current disk space = 3052602703872 free memory = 1576422716 SRR26075339 SRAfilesize 072aff0c6e8f42e7b02a062ad9058da8 SRR26075339.sra SRR26075339.sra file validated SRR26075339 is paired end SRR26075339 is conventional basespace SRR26075339 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075339_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.52775 37.0 37.0 37.0 37.0 37.0 2 36.5325 37.0 37.0 37.0 37.0 37.0 3 36.524 37.0 37.0 37.0 37.0 37.0 4 36.631 37.0 37.0 37.0 37.0 37.0 5 36.6905 37.0 37.0 37.0 37.0 37.0 6 36.6075 37.0 37.0 37.0 37.0 37.0 7 36.5465 37.0 37.0 37.0 37.0 37.0 8 36.65 37.0 37.0 37.0 37.0 37.0 9 36.6045 37.0 37.0 37.0 37.0 37.0 10-14 36.6155 37.0 37.0 37.0 37.0 37.0 15-19 36.5659 37.0 37.0 37.0 37.0 37.0 20-24 36.5068 37.0 37.0 37.0 37.0 37.0 25-29 36.4388 37.0 37.0 37.0 37.0 37.0 30-34 36.37820000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.3673 37.0 37.0 37.0 37.0 37.0 40-44 36.2596 37.0 37.0 37.0 37.0 37.0 45-49 36.2105 37.0 37.0 37.0 37.0 37.0 50-54 36.1463 37.0 37.0 37.0 37.0 37.0 55-59 36.088699999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.06250000000001 37.0 37.0 37.0 37.0 37.0 65-69 35.9584 37.0 37.0 37.0 37.0 37.0 70-74 36.0026 37.0 37.0 37.0 37.0 37.0 75-79 35.9747 37.0 37.0 37.0 37.0 37.0 80-84 36.007999999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.8866 37.0 37.0 37.0 37.0 37.0 90-94 35.8125 37.0 37.0 37.0 37.0 37.0 95-99 35.8467 37.0 37.0 37.0 37.0 37.0 100-104 35.809000000000005 37.0 37.0 37.0 37.0 37.0 105-109 35.6971 37.0 37.0 37.0 37.0 37.0 110-114 35.4938 37.0 37.0 37.0 37.0 37.0 115-119 35.4703 37.0 37.0 37.0 37.0 37.0 120-124 35.4596 37.0 37.0 37.0 34.6 37.0 125-129 35.3818 37.0 37.0 37.0 34.6 37.0 130-134 35.2806 37.0 37.0 37.0 32.2 37.0 135-139 35.2928 37.0 37.0 37.0 32.2 37.0 140-144 35.1462 37.0 37.0 37.0 27.4 37.0 145-149 35.17040000000001 37.0 37.0 37.0 27.4 37.0 150-151 35.10825 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 2.0 21 1.0 22 2.0 23 3.0 24 5.0 25 5.0 26 12.0 27 17.0 28 27.0 29 22.0 30 30.0 31 45.0 32 79.0 33 83.0 34 179.0 35 452.0 36 2855.0 37 180.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.89917231000752 11.98896413343366 5.894156007022825 36.217707549535994 2 17.525 13.225000000000001 35.449999999999996 33.800000000000004 3 17.299999999999997 17.175 31.924999999999997 33.6 4 21.925 22.425 25.724999999999998 29.925 5 25.324999999999996 30.175 23.674999999999997 20.825 6 22.400000000000002 33.75 23.875 19.975 7 16.150000000000002 26.325 40.0 17.525 8 18.475 27.575 30.425 23.525 9 18.475 23.45 35.325 22.75 10-14 19.901990199019902 29.392939293929395 27.632763276327633 23.072307230723073 15-19 20.645 27.455000000000002 26.834999999999997 25.064999999999998 20-24 20.22 27.58 28.065 24.135 25-29 19.96 28.155 27.950000000000003 23.935000000000002 30-34 21.12 27.6 27.405 23.875 35-39 20.810000000000002 27.450000000000003 27.855 23.885 40-44 21.325 27.72 27.26 23.695 45-49 20.585 28.415000000000003 26.645000000000003 24.355 50-54 20.285 27.405 27.839999999999996 24.47 55-59 21.73 27.639999999999997 26.889999999999997 23.74 60-64 19.99 26.955000000000002 28.565 24.490000000000002 65-69 20.849999999999998 27.18 27.334999999999997 24.635 70-74 21.305 26.905 27.88 23.91 75-79 20.955 27.439999999999998 27.54 24.065 80-84 21.19 27.515 26.8 24.495 85-89 20.880000000000003 28.08 27.155 23.885 90-94 21.105 28.455000000000002 27.04 23.400000000000002 95-99 21.005 27.18 27.305 24.51 100-104 20.424999999999997 27.779999999999998 27.38 24.415 105-109 21.325 27.139999999999997 27.465 24.07 110-114 20.615 28.060000000000002 27.105 24.22 115-119 21.265 27.889999999999997 27.68 23.165 120-124 21.04 27.775 27.150000000000002 24.035 125-129 21.305 27.41 27.229999999999997 24.055 130-134 22.255 27.089999999999996 26.974999999999998 23.68 135-139 22.465 27.715 26.13 23.69 140-144 21.475 27.6 26.415 24.51 145-149 22.035 27.965 25.395 24.605 150-151 21.6125 29.049999999999997 26.125 23.2125 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.5 21 1.0 22 1.0 23 1.5 24 1.0 25 0.0 26 2.0 27 6.0 28 11.0 29 13.0 30 15.5 31 21.5 32 27.5 33 36.0 34 42.5 35 46.5 36 63.5 37 77.5 38 105.0 39 144.5 40 168.0 41 210.0 42 229.5 43 224.5 44 236.5 45 276.5 46 287.0 47 265.5 48 245.5 49 212.5 50 171.0 51 140.0 52 137.0 53 113.0 54 102.5 55 96.5 56 67.5 57 46.5 58 30.0 59 21.5 60 16.0 61 10.5 62 10.0 63 11.5 64 13.0 65 9.0 66 5.0 67 4.5 68 2.5 69 1.0 70 1.5 71 3.0 72 4.5 73 3.5 74 1.0 75 1.5 76 1.5 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.325 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.925 #Duplication Level Percentage of deduplicated Percentage of total 1 68.0400462071621 44.175 2 19.29149018097805 25.05 3 7.354639969195226 14.325 4 3.118983442433577 8.1 5 1.1166730843280708 3.6249999999999996 6 0.6160954948016942 2.4 7 0.2695417789757413 1.225 8 0.07701193685021178 0.4 9 0.07701193685021178 0.44999999999999996 >10 0.03850596842510589 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCAACACGTTGAGAGTAACGGCCTTTCTTCAAGTATCGACCGATCCGACC 10 0.25 No Hit GGGACTTGTTGATGAAGGTTGAGGCATTAGACCACAAGAACAGTAACGCA 9 0.22499999999999998 No Hit ACTCCATATAATTTGCTAAATACAGACTAATTAATTAATTAATACTCCAC 9 0.22499999999999998 No Hit CACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCT 8 0.2 No Hit CTTGTCTGTCAGCCAGTCAACCTTGCGGCGGGTGTTAACAAAAATAACAC 8 0.2 No Hit GTTGCACGAAAGCGCTTAGATATATATTATACAAGTACTAGCATGATCAC 7 0.17500000000000002 No Hit CCCCACATATATATAAATTTAAAAAAGATCTTAGCAAAATGGGGGATTGA 7 0.17500000000000002 No Hit CCGTGGCAAAAATGCGGCCGTCAGGATGCCAAGCAGATGCAAATGAGAAA 7 0.17500000000000002 No Hit GCTTCCATCTCTTCCTTCTACACAAACTACAACAGCCTCTGTCTCACCCG 7 0.17500000000000002 No Hit CTCCATTCTCTGGATAATTGAAATCTCCAAATACCAGTTTACCTTTCTCC 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGCTCAAATCTCGGTT 7 0.17500000000000002 TruSeq Adapter, Index 10 (97% over 38bp) GTAAAGTATGATGGAAAGCTTCTTTGTAGGGGATGATAGTGATATTGAAG 7 0.17500000000000002 No Hit GTGCGATTTTGCAGTTGCTTCTCTCGATTTCAGCAATGGGGTCTATCAAA 6 0.15 No Hit GTGACAACAAGGGTGCTGAATATAGTGAAAATTAAATAGGTAAATGAACC 6 0.15 No Hit AGTCAAGAAAGAACACCTAGAATACATACCTCCTACAAAAAAAGAAAAAA 6 0.15 No Hit CCTTGAGCGCTTTAGTGGCAGCATTATACCAGGATGTGGTGATGGGAAGC 6 0.15 No Hit GTTGATAACTTGCTCAGTGCCAAATGCTCTCATTGGGCTTCTAGGACTCG 6 0.15 No Hit GTCATAATCAGTAGGTAGATCAGTAGTAGGAAGGATCGGCGCCTTGTTAG 6 0.15 No Hit GGTTTCCACGTATCAAGTGGACATTCTCAGGATACTCAATCTTTAGAGCA 6 0.15 No Hit CTGCAGAAAAAATCTCAAATCATCTTCTGCTTTCCGCCAACAACCAAACT 6 0.15 No Hit AAGCGAAGGAATGCAATTTTGCAATTGCTTGTCTCAATCTCAGCTGTAGG 6 0.15 No Hit CTTTCCACTGCCTTCTGAGCTTCATCAGCGTATTTGTAGCGCACAAATGC 6 0.15 No Hit AGCTAGCATGACAAGACAGAGAGCGAGATATTCTTCTTCAGTAGGCTGGT 6 0.15 No Hit GTTCCGCTATATCGATTCTTACAGTCTTCCCAGCAACGATTTTGACCTTT 6 0.15 No Hit CCCAGAATACAAGTATTATTTACAGTCAATGTGCTCACAGGTTCACAATG 6 0.15 No Hit CCCATGAGCAATCAACGACAGCCAATCCTTTCCTTTTAATTAAGTTATAA 6 0.15 No Hit GCTATGTTCCTTGAATATCTTTATCATTTCAAGCCCTTGCTCTAACTTGA 6 0.15 No Hit GTCTGTTTCAGCTAAAGCGTCCTTTCCAACAAGTTGGACAATTTCATTCA 6 0.15 No Hit CTTGTGTCTCCCCCTTGTGCACGCCACCGCCTCCCCCCGGGATCTTGTCC 5 0.125 No Hit GCAATGGCTGGATCAATGTACCCTGGAATTTCAAGATGTCGATTTTGGAA 5 0.125 No Hit CACTTTTCCTTCAGCTTGAAAAGCCTGGGCAGAAGCCTTCAAGTCCCTTT 5 0.125 No Hit CCTCTCTCTTTGTATCCAAAACACAAACAGATGCCTTCTTTTCTTCAGAT 5 0.125 No Hit TACCAGTTTACCTTTCTCCGATCCGAATTTCTTCCAGTTCCAAACTCCTG 5 0.125 No Hit GGCGAAGAACACTCATGCGTGAGCGATGGACTATCATGAGCATAAGTAAA 5 0.125 No Hit GTCAGAGACAAGAAAGGTGGAGCCAGCGAGCTGAAGCTGAGCGGAGACGA 5 0.125 No Hit GTTTCAGGTTCTTAAAACCAGTGTATGCCTCAAGCATATTTTCTAGGACT 5 0.125 No Hit TGTCTCCAAATATGGCCACACATCAAACCTTGAGATCCATTTACGGAGAA 5 0.125 No Hit GGCTGCATTTTGTTTTGGTTACAGTAATGGATTGCATATTGTTGGGACAC 5 0.125 No Hit GTCTCTTCAGCTCTTTGGCCGCCATCAATCCTTCTTTTCCCATCTCCTTC 5 0.125 No Hit GCCCACTTGAAGAGTTTCCAGTAAATTTTCAGTCATTTCCTCTTACCTCA 5 0.125 No Hit CGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTT 5 0.125 No Hit GGGCAAATCTTTGCAAAGCCGTAGGTAATGAAATTTCCTCACTGATTTTC 5 0.125 No Hit ACTTGATAATGTCAATGAACTCCGGCTTTAGGGAAGCACCACCAACCAAA 5 0.125 No Hit CGCACAAGCCATTGCCATACACACAAAGTAGCAATTCCTTAAAAATAGAC 5 0.125 No Hit GCCCCAACTGCCATCGTCAAAGTATTGTACTGGCAAACTCTTTTGAAGAG 5 0.125 No Hit GAACAGTAAGTAATTCATCAAAGGCTATGCCGCGCCTTTCTGTCTCACCT 5 0.125 No Hit ACCTGTTGCTGAACCAAGCTGTGCTAACTGTCTCCTCCTATCCATTTGTT 5 0.125 No Hit GTGATACTTCTTTATTACTGCTGAAGCTACTTAGGTTAGAATCTGAAGAT 5 0.125 No Hit CTCCAACCTGAGTTCTTAGACTCACCAACAGTGGAAAATCCACCGGATGC 5 0.125 No Hit GAAGAAGATTACAGACCTGCTCTAAGATCTTAATGAAAACTTGTTAGCCA 5 0.125 No Hit CTTTCTTCTGCTTTGGAAAAAGATAATAAAACCAACAATCAGGCCCAACA 5 0.125 No Hit GGCAAGTCCAATGGCCTCAGACCCTTTCATGATCCTCAAGCGCTTGCATG 5 0.125 No Hit ATTAGAACCCTCACCGTCATTGACCTGGATTCTGGTGAGCTTGGGAGCCT 5 0.125 No Hit AATGACCATGACCGGTCCTGCTGCAACCAGTGATCCAACCACACTTCCTC 5 0.125 No Hit GCTAGGATATGGAGGAAGCAAGAGATCATTGTTATGCTCCACAGTTTCCC 5 0.125 No Hit CACTCGTCGTGTCCGGCGTAGTGAAGAAGGCTTGACACACATACTCAGTC 5 0.125 No Hit GTTCTTGTAAGCCTCCCTCAATACAGCCTTCTGCTTCTGCGAAATCACAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.05 0.0 0.0 0.0 0.0 86-87 0.05 0.0 0.0 0.0 0.0 88-89 0.05 0.0 0.0 0.0 0.0 90-91 0.0625 0.0 0.0 0.0 0.0 92-93 0.075 0.0 0.0 0.0 0.0 94-95 0.125 0.0 0.0 0.0 0.0 96-97 0.125 0.0 0.0 0.0 0.0 98-99 0.16249999999999998 0.0 0.0 0.0 0.0 100-101 0.175 0.0 0.0 0.0 0.0 102-103 0.375 0.0 0.0 0.0 0.0 104-105 0.375 0.0 0.0 0.0 0.0 106-107 0.4375 0.0 0.0 0.0 0.0 108-109 0.6625000000000001 0.0 0.0 0.0 0.0 110-111 0.8375 0.0 0.0 0.0 0.0 112-113 1.0375 0.0 0.0 0.0 0.0 114-115 1.3125 0.0 0.0 0.0 0.0 116-117 1.675 0.0 0.0 0.0 0.0 118-119 1.9625 0.0 0.0 0.0 0.0 120-121 2.3875 0.0 0.0 0.0 0.0 122-123 2.6125 0.0 0.0 0.0 0.0 124-125 2.8375 0.0 0.0 0.0 0.0 126-127 3.2125000000000004 0.0 0.0 0.0 0.0 128-129 3.95 0.0 0.0 0.0 0.0 130-131 4.375 0.0 0.0 0.0 0.0 132-133 4.85 0.0 0.0 0.0 0.0 134-135 5.362500000000001 0.0 0.0 0.0 0.0 136-137 6.225 0.0 0.0 0.0 0.0 138-139 6.8 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACATTT 10 0.006830828 145.0 4 GCCCACA 10 0.006830828 145.0 1 TTCAAGG 10 0.006830828 145.0 8 CAGTATT 10 0.006830828 145.0 2 ACATTTG 10 0.006830828 145.0 5 AGTATTT 10 0.006830828 145.0 3 GCAGTAT 10 0.006830828 145.0 1 TCCACTT 10 0.006830828 145.0 2 ATTTGGC 10 0.006830828 145.0 145 >>END_MODULE SRR26075339 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075339_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2665 37.0 37.0 37.0 37.0 37.0 2 36.1945 37.0 37.0 37.0 37.0 37.0 3 36.253 37.0 37.0 37.0 37.0 37.0 4 36.303 37.0 37.0 37.0 37.0 37.0 5 36.1965 37.0 37.0 37.0 37.0 37.0 6 36.1445 37.0 37.0 37.0 37.0 37.0 7 36.033 37.0 37.0 37.0 37.0 37.0 8 36.176 37.0 37.0 37.0 37.0 37.0 9 36.163 37.0 37.0 37.0 37.0 37.0 10-14 36.073299999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.0603 37.0 37.0 37.0 37.0 37.0 20-24 35.9028 37.0 37.0 37.0 37.0 37.0 25-29 35.7722 37.0 37.0 37.0 37.0 37.0 30-34 35.664699999999996 37.0 37.0 37.0 37.0 37.0 35-39 35.6188 37.0 37.0 37.0 37.0 37.0 40-44 35.6411 37.0 37.0 37.0 37.0 37.0 45-49 35.4907 37.0 37.0 37.0 37.0 37.0 50-54 35.342 37.0 37.0 37.0 37.0 37.0 55-59 35.3892 37.0 37.0 37.0 37.0 37.0 60-64 35.4572 37.0 37.0 37.0 37.0 37.0 65-69 35.393 37.0 37.0 37.0 37.0 37.0 70-74 35.284800000000004 37.0 37.0 37.0 37.0 37.0 75-79 35.209199999999996 37.0 37.0 37.0 37.0 37.0 80-84 35.2238 37.0 37.0 37.0 37.0 37.0 85-89 35.2641 37.0 37.0 37.0 37.0 37.0 90-94 35.103300000000004 37.0 37.0 37.0 32.2 37.0 95-99 35.1169 37.0 37.0 37.0 34.6 37.0 100-104 35.044900000000005 37.0 37.0 37.0 27.4 37.0 105-109 35.0253 37.0 37.0 37.0 32.2 37.0 110-114 34.987899999999996 37.0 37.0 37.0 25.0 37.0 115-119 34.9163 37.0 37.0 37.0 25.0 37.0 120-124 34.808800000000005 37.0 37.0 37.0 25.0 37.0 125-129 34.8692 37.0 37.0 37.0 25.0 37.0 130-134 34.8451 37.0 37.0 37.0 25.0 37.0 135-139 34.5991 37.0 37.0 37.0 25.0 37.0 140-144 34.7294 37.0 37.0 37.0 25.0 37.0 145-149 34.6595 37.0 37.0 37.0 25.0 37.0 150-151 34.434 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 10.0 14 13.0 15 14.0 16 10.0 17 9.0 18 11.0 19 12.0 20 12.0 21 13.0 22 11.0 23 16.0 24 17.0 25 16.0 26 18.0 27 18.0 28 12.0 29 17.0 30 29.0 31 39.0 32 43.0 33 89.0 34 170.0 35 639.0 36 2564.0 37 197.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.525 26.450000000000003 8.3 19.725 2 28.325 27.1 26.950000000000003 17.625 3 25.4 29.299999999999997 26.674999999999997 18.625 4 26.05 34.375 20.974999999999998 18.6 5 24.7 37.25 20.275000000000002 17.775 6 23.775 36.775000000000006 21.725 17.724999999999998 7 23.974999999999998 22.275 34.150000000000006 19.6 8 23.925 25.55 25.5 25.025 9 23.35 25.275 29.475 21.9 10-14 26.584999999999997 28.444999999999997 24.425 20.544999999999998 15-19 25.635 27.534999999999997 26.155 20.674999999999997 20-24 24.905 28.265 26.52 20.31 25-29 25.674999999999997 27.595 25.935000000000002 20.794999999999998 30-34 25.485000000000003 28.384999999999998 25.629999999999995 20.5 35-39 25.555 27.495000000000005 26.58 20.369999999999997 40-44 25.419999999999998 28.425 25.990000000000002 20.165 45-49 25.595000000000002 26.915 26.735 20.755000000000003 50-54 24.779999999999998 27.49 27.055 20.674999999999997 55-59 25.525 27.515 26.31 20.65 60-64 25.590000000000003 27.655 26.529999999999998 20.225 65-69 25.115 27.565 26.165 21.154999999999998 70-74 25.240000000000002 28.035 26.61 20.115 75-79 24.05 28.825 25.88 21.245 80-84 25.115 28.249999999999996 26.185000000000002 20.45 85-89 26.265 27.905 25.31 20.52 90-94 24.5 28.625 25.81 21.065 95-99 25.130000000000003 28.22 26.400000000000002 20.25 100-104 25.19 28.605000000000004 25.945 20.26 105-109 25.779999999999998 27.800000000000004 26.41 20.01 110-114 25.165 28.87 26.125 19.84 115-119 25.14 28.415000000000003 26.745 19.7 120-124 25.974999999999998 27.92 25.81 20.294999999999998 125-129 25.415 28.915000000000003 25.759999999999998 19.91 130-134 26.590000000000003 28.060000000000002 26.305 19.045 135-139 26.305 29.07 25.080000000000002 19.545 140-144 26.91 28.33 25.374999999999996 19.384999999999998 145-149 27.525 28.49 24.82 19.165 150-151 27.2625 27.712500000000002 25.624999999999996 19.400000000000002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.0 7 1.5 8 1.5 9 1.0 10 0.5 11 0.5 12 1.0 13 0.5 14 0.5 15 2.0 16 1.5 17 0.5 18 2.5 19 2.0 20 1.0 21 2.0 22 3.0 23 2.5 24 2.0 25 2.0 26 2.0 27 2.0 28 2.5 29 8.0 30 9.5 31 11.0 32 10.5 33 16.5 34 24.0 35 26.0 36 51.0 37 83.5 38 103.0 39 126.5 40 168.0 41 220.5 42 239.5 43 248.5 44 288.5 45 315.0 46 306.0 47 290.5 48 250.0 49 198.5 50 173.0 51 149.0 52 123.0 53 95.0 54 67.5 55 56.0 56 45.5 57 32.0 58 28.0 59 18.0 60 12.5 61 20.0 62 16.5 63 7.0 64 9.0 65 10.0 66 5.5 67 4.0 68 5.5 69 4.0 70 3.0 71 3.5 72 3.0 73 1.5 74 1.5 75 1.0 76 1.5 77 2.5 78 2.0 79 1.5 80 3.0 81 2.5 82 3.0 83 4.5 84 2.0 85 1.0 86 2.0 87 2.5 88 1.0 89 0.5 90 1.5 91 1.5 92 2.0 93 2.5 94 1.0 95 3.0 96 3.5 97 1.0 98 1.5 99 2.0 100 13.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.075 #Duplication Level Percentage of deduplicated Percentage of total 1 69.22781406069919 45.050000000000004 2 19.016519400691507 24.75 3 6.607760276603919 12.9 4 2.996542451018056 7.8 5 0.9988474836726854 3.25 6 0.5378409527468306 2.1 7 0.3073376872839032 1.4000000000000001 8 0.11525163273146369 0.6 9 0.0768344218209758 0.44999999999999996 >10 0.11525163273146369 1.7000000000000002 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 43 1.075 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 15 0.375 No Hit GCTAGGTTTCTAATTGTCTGTGTTTACTGTGCCTGTGAGAGAGAGACTTC 10 0.25 No Hit GTGTATTGTAAATTGACACTACCCAAACACGACGTGGCATGTTACGATTC 9 0.22499999999999998 No Hit CATTTTCCTATGGAGGAACAAGAAGATATCAGCAGGAGTGCTTGGTGGTG 9 0.22499999999999998 No Hit ATTTTCCAGTTGCTGCCATCTAAAGTTCAAGTTGGTGTGTTCTCCGCTAC 8 0.2 No Hit ACGGAGTAATAGAAGGGGTCATCGATCTCGACCAATAAGCATCACATGTT 8 0.2 No Hit AGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCC 8 0.2 No Hit GTTAGGCTTGAGCAGATTCATTCGCCAACTAACCCTTTAATTTATCCTAT 7 0.17500000000000002 No Hit TGGTACTGTTCTGTTGATCTATGTTAGTTTGAGTAAAGAGTTGACATTTT 7 0.17500000000000002 No Hit TGACAGTGGAGTGAGAAACTTTGACATGGAGAGATTTCAGCAAACTAAGC 7 0.17500000000000002 No Hit AAGGAAACCAAATACTTGTAGCAAAAATGGGTTCTCTCCCTCATGTAGTA 7 0.17500000000000002 No Hit AGTTCAGTTGCAGAAAGCAGAAGCCAACAACAATTTCTCTGCGATGGCCA 7 0.17500000000000002 No Hit AACCAGTGGGCATTTGAATGAAGTGCGTCTGATGGTTTCATGGAAGGAAT 7 0.17500000000000002 No Hit CTTGAAGATGGTGAAACTGTCTACTTTCTGTTTCCTGTTTGTCCTCTTCC 7 0.17500000000000002 No Hit TTGGATAAGAAAACAATGGTTCCAGGTGATGTTTCTGTGCCGCAGGCTGT 7 0.17500000000000002 No Hit GTGACTTAATGCGTCTATTTGATGAATATGGATTTCCATCCACAGCAGGA 6 0.15 No Hit TCAATTGGCACCCAAGAAAGTCACTCCTCGAGTTACAGGAGGTTTTGGGT 6 0.15 No Hit AGAAAACTTAGTTCAGGTTCAGAAGCTCAATGGCACAAATTGTTTTGTGA 6 0.15 No Hit GGAATATTGGCAACAAGACAAGTGGACAGGCTATTTCCCTGTGAAGTGGC 6 0.15 No Hit AAAGTACTATCCGTCTGCTCAATCCACTTCACACAATGTCGAGTATCAAT 6 0.15 No Hit GCCATTCTTTCCTTGAAGTAATGGCTAATTGGATATAGTATACACAGGGC 6 0.15 No Hit CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAA 6 0.15 No Hit GGCTATTGGAACAGGAAAGGTTGCAAGTCCTGCTCAGGCACAGGAGGTGC 6 0.15 No Hit CCATTTGAAGCAATTCGCAACAACACCAACCTGTGTGGCAATGCTACTGG 6 0.15 No Hit CCCACTCACATCTGACTATGAAAATGGGAACAGAACTGCTGTGAACAGCC 6 0.15 No Hit CATAGATCTACTCTTCATGGATCATCCTTCGATCTTTTCAATTGCAGCTT 6 0.15 No Hit CTTTCTGAACCGCAAACCCTAACTCTCTCTGCAAAAGATGTCTCACTTCG 6 0.15 No Hit GTATCTTCCAACGACCTCGACGATCCTTTAATCATACAAGTCGTATCAGA 6 0.15 No Hit GTGGAACCTAGGCGCGTGGTTCTGTATTTATCTTATCATCCTTGTTTATC 6 0.15 No Hit GGAAAGAGTCAGTGGAACGGAACACACTCACATTCTGCGAGTACCATTTA 5 0.125 No Hit CTAGATTATGATGAATATAAGATGAAGTTAAAGCTTCAGGAACTAGAGAG 5 0.125 No Hit GGCAACACTAGCTGATTCTTTCCTTGCTGACCTTGATGAGTTATCCGACA 5 0.125 No Hit GTATGTGCCAACTTATGAAGACAAGGATGGAGATTGGATGCTTGTAGGAG 5 0.125 No Hit AATTTTTTTATTCTCAACAAAATATAGTAGTTTTCTTTTTTGGGTTTTCA 5 0.125 No Hit GGACAGTTCAAGCGAAGAATAGGATTCTATTAGTGGTTCAGATCGAGAAG 5 0.125 No Hit GAGCATACTTCACTTGCTCTCAGTGTTTCTTCAATGATGATGATTCAGTT 5 0.125 No Hit GAGGGTGAGAGCCCCGTCGTGGCTGGACCCTGCCGCACAACGAGGCGCTG 5 0.125 No Hit GAACGTGCTGGTAAAGTAAAATGCCTTGGTTCGCCAGAGCGTACGGGTAG 5 0.125 No Hit AATCCTTCTAGTTAGATTCAGCATTCACGGAGTCATCTATTTTGGGAATG 5 0.125 No Hit GCTGGAACGCCTGAATGGATGGCACCTGAAGTCCTGAGAAATGAACCAGC 5 0.125 No Hit ATTGCACTTCCGTCACCTGAGGAGATGATGGAAGATGTTAAAGCCTTCTA 5 0.125 No Hit CTTTGAACGTATTGCAAGATTTCCAAGCTGTGAATTCTGATCATGTGTTC 5 0.125 No Hit AGGAAATCCACTCTCTTCTTGACAAGCTCCCACCCGAGACCATCATGCTG 5 0.125 No Hit ATTGGCCAAGATCATCAGCTTATGTGCAACCACTACACTTTGAGACAAGA 5 0.125 No Hit CTTGAAGGAGGAGTTGCATTTGACAGGGTCCATGGAGCTCACGCCTTTGA 5 0.125 No Hit GGAAAAGCCTCCCTGTCTAGAAAACCCCATTCTCTCACTCAAAAACATCA 5 0.125 No Hit AGAACCCTTTTCCTTCTCTCTCTACTTGTTTCTTCCTCTCTCCACAGCTA 5 0.125 No Hit GGTAATTATCTGGCCATTGCAACCACGTTCAGTGCGGCAGGAAGTGTCGC 5 0.125 No Hit AATCAAGGTTCATGAGTTGAGACAGAAATCAAAGACAGATCTTTTGTCTC 5 0.125 No Hit ACAAAAACAAGCTCTCTCTCTCTTCTCTCTCTTCTCTCTCTCTAGAGTAA 5 0.125 No Hit GTAATATTGACGTTTTCATGGGTGAAAAGAGACCATCGCTTCCTCACGAG 5 0.125 No Hit GGCAAAGGGGTGTGCCCTGTGGTAGGTTGCAGGGCCAATCTGCTGTCCAC 5 0.125 No Hit GGAAGGAGATGATGATGAGACTGAGAGGCTTTTGCGTATCCGTGATGCTC 5 0.125 No Hit ACTCAGTAAATGTGGCTACAGCTGTGCAAATTCTCTTGCTTAGATTTCGT 5 0.125 No Hit AGAAGAAAGAAATACACAATGGCAGGAATCATGCACAAGATTGAGGAGAC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.05 0.0 0.0 0.0 0.0 86-87 0.05 0.0 0.0 0.0 0.0 88-89 0.05 0.0 0.0 0.0 0.0 90-91 0.0625 0.0 0.0 0.0 0.0 92-93 0.075 0.0 0.0 0.0 0.0 94-95 0.125 0.0 0.0 0.0 0.0 96-97 0.125 0.0 0.0 0.0 0.0 98-99 0.16249999999999998 0.0 0.0 0.0 0.0 100-101 0.175 0.0 0.0 0.0 0.0 102-103 0.375 0.0 0.0 0.0 0.0 104-105 0.375 0.0 0.0 0.0 0.0 106-107 0.4375 0.0 0.0 0.0 0.0 108-109 0.6625000000000001 0.0 0.0 0.0 0.0 110-111 0.8500000000000001 0.0 0.0 0.0 0.0 112-113 1.0625 0.0 0.0 0.0 0.0 114-115 1.3375 0.0 0.0 0.0 0.0 116-117 1.7 0.0 0.0 0.0 0.0 118-119 1.975 0.0 0.0 0.0 0.0 120-121 2.3875 0.0 0.0 0.0 0.0 122-123 2.6125 0.0 0.0 0.0 0.0 124-125 2.8375 0.0 0.0 0.0 0.0 126-127 3.2125000000000004 0.0 0.0 0.0 0.0 128-129 3.9625000000000004 0.0 0.0 0.0 0.0 130-131 4.4 0.0 0.0 0.0 0.0 132-133 4.875 0.0 0.0 0.0 0.0 134-135 5.3375 0.0 0.0 0.0 0.0 136-137 6.175000000000001 0.0 0.0 0.0 0.0 138-139 6.75 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAAAAG 10 0.006830828 145.0 8 TTTTCCA 10 0.006830828 145.0 4 TTCCAAA 10 0.006830828 145.0 6 ATGATGA 30 4.189703E-5 29.000002 75-79 >>END_MODULE Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058988 spots for SRR26075339.sra Written 3058988 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra Read 3058984 spots for SRR26075339.sra Written 3058984 spots for SRR26075339.sra SRR ids: ['SRR26075339.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_zbu3tovu SRR26075339.sra spots: 61179684 blocks: [[1, 3058984], [3058985, 6117968], [6117969, 9176952], [9176953, 12235936], [12235937, 15294920], [15294921, 18353904], [18353905, 21412888], [21412889, 24471872], [24471873, 27530856], [27530857, 30589840], [30589841, 33648824], [33648825, 36707808], [36707809, 39766792], [39766793, 42825776], [42825777, 45884760], [45884761, 48943744], [48943745, 52002728], [52002729, 55061712], [55061713, 58120696], [58120697, 61179684]] SRR26075339 file size 22600871 SRR26075339 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075339 SRR26075339_1.fastq SRR26075339_2.fastq Input file: SRR26075339_1.fastq Paired file: SRR26075339_2.fastq trimmed: SRR26075339-trimmed-pair1.fastq, SRR26075339-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 21:43:31 2025 >> started Tue Feb 11 21:45:06 2025 >> done (95.248s) 61179684 read pairs processed; of these: 441 ( 0.00%) short read pairs filtered out after trimming by size control 221507 ( 0.36%) empty read pairs filtered out after trimming by size control 60957736 (99.64%) read pairs available; of these: 6749981 (11.07%) trimmed read pairs available after processing 54207755 (88.93%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 34 0.00% 19 42 0.00% 20 38 0.00% 21 58 0.00% 22 58 0.00% 23 88 0.00% 24 93 0.00% 25 94 0.00% 26 83 0.00% 27 102 0.00% 28 89 0.00% 29 85 0.00% 30 88 0.00% 31 119 0.00% 32 109 0.00% 33 85 0.00% 34 134 0.00% 35 108 0.00% 36 121 0.00% 37 106 0.00% 38 120 0.00% 39 174 0.00% 40 158 0.00% 41 188 0.00% 42 134 0.00% 43 173 0.00% 44 198 0.00% 45 189 0.00% 46 204 0.00% 47 211 0.00% 48 218 0.00% 49 221 0.00% 50 253 0.00% 51 300 0.00% 52 291 0.00% 53 277 0.00% 54 347 0.00% 55 389 0.00% 56 392 0.00% 57 476 0.00% 58 609 0.00% 59 494 0.00% 60 559 0.00% 61 539 0.00% 62 660 0.00% 63 728 0.00% 64 758 0.00% 65 858 0.00% 66 911 0.00% 67 835 0.00% 68 1018 0.00% 69 1060 0.00% 70 1172 0.00% 71 1260 0.00% 72 1452 0.00% 73 1499 0.00% 74 1770 0.00% 75 1981 0.00% 76 2080 0.00% 77 2251 0.00% 78 2500 0.00% 79 2843 0.00% 80 3082 0.01% 81 3370 0.01% 82 3682 0.01% 83 4222 0.01% 84 4632 0.01% 85 5005 0.01% 86 5422 0.01% 87 5987 0.01% 88 6470 0.01% 89 7007 0.01% 90 7561 0.01% 91 8881 0.01% 92 9874 0.02% 93 10813 0.02% 94 11700 0.02% 95 13226 0.02% 96 14154 0.02% 97 15781 0.03% 98 17269 0.03% 99 18751 0.03% 100 20120 0.03% 101 21717 0.04% 102 24602 0.04% 103 27154 0.04% 104 29845 0.05% 105 31966 0.05% 106 34989 0.06% 107 36970 0.06% 108 40379 0.07% 109 43844 0.07% 110 45149 0.07% 111 50020 0.08% 112 53693 0.09% 113 57481 0.09% 114 62112 0.10% 115 67834 0.11% 116 72468 0.12% 117 76840 0.13% 118 82344 0.14% 119 85470 0.14% 120 90246 0.15% 121 96141 0.16% 122 101685 0.17% 123 109080 0.18% 124 115077 0.19% 125 120501 0.20% 126 128359 0.21% 127 133585 0.22% 128 140110 0.23% 129 147229 0.24% 130 150943 0.25% 131 154775 0.25% 132 163202 0.27% 133 168956 0.28% 134 174434 0.29% 135 182956 0.30% 136 188382 0.31% 137 195294 0.32% 138 201363 0.33% 139 205685 0.34% 140 208779 0.34% 141 217080 0.36% 142 221336 0.36% 143 226317 0.37% 144 234556 0.38% 145 240738 0.39% 146 243401 0.40% 147 249539 0.41% 148 251582 0.41% 149 254808 0.42% 150 257472 0.42% 151 54207755 88.93% 60957736 reads passed initial QC criterion=sequence-density sequence-density=0.42 sequence-density-rank=1 fanout-score=3.64 fanout-score-rank=20 prefix-density=0.44 prefix-fanout=3.4 sequence=CCACATTTGCAGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=35 fanout-score=19.90 fanout-score-rank=1 prefix-density=0.13 prefix-fanout=2.4 sequence=CGCATCGCCGGCCCCCATCCACTTCCCTCCCGACAATTTCAAGCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTCCCTCGCGGTACTTGTTTGCTATCGGTCTCTCGCCCGTATTTAGCCTTGGACGGAATTTACCGCCCGATTGGGGCTGCATTCCCAAACAACCCGACTCGCAGACAGCGCCTCGTGGTGCGGCAGGGTCCAGCCACGACGGGGCTCTCACCCTCTCCGGCGCCCCTTTCCAGGGGACTTGGGCCTGGTCCGCCGCTGAGGACGCTTCTCCAGACTACAATTCGGACGCCGCAGGCGCCAGATTCTCAAGCTGGGCATTTCCCGGTTCGCTCGCCGTTACTAGGGGAATCCTTGTAAGTTTCTTTTCCTCCGCTTATTGATATGCTTAAACTCAGCGGGTAGTCCCGCCTGAC criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=3.71 fanout-score-rank=21 prefix-density=0.39 prefix-fanout=3.3 sequence=GCTGCAAATGTGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=260.90 fanout-score-rank=1 prefix-density=0.15 prefix-fanout=13.2 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAGAACGTGGCCTTGG SRR26075339 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 21:46:04 Started mapping on | Feb 11 21:46:05 Finished on | Feb 11 21:57:12 Mapping speed, Million of reads per hour | 329.01 Number of input reads | 60957736 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 52307662 Uniquely mapped reads % | 85.81% Average mapped length | 296.33 Number of splices: Total | 49415639 Number of splices: Annotated (sjdb) | 48207310 Number of splices: GT/AG | 48490285 Number of splices: GC/AG | 686464 Number of splices: AT/AC | 45930 Number of splices: Non-canonical | 192960 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 2.96 Insertion rate per base | 0.02% Insertion average length | 2.61 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1641663 % of reads mapped to multiple loci | 2.69% Number of reads mapped to too many loci | 1236728 % of reads mapped to too many loci | 2.03% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.63% % of reads unmapped: other | 0.84% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 7008411 7008411 7008411 N_multimapping 1641663 1641663 1641663 N_noFeature 1429282 51748225 1715438 N_ambiguous 619432 5347 342224 UnstrandedReadsAssigned:50258948 PositiveStrandReadsAssigned:554090 NegativeStrandReadsAssigned:50250000 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075339 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075339-trimmed-pair1.fastq SRR26075339-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 60,957,736 reads, 51,922,273 reads pseudoaligned [quant] estimated average fragment length: 224.564 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,209 rounds 52401 SRR26075339.ke.tsv 34699 SRR26075339.se.tsv 87100 total ==> SRR26075339.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1794.44 5512 48.9474 Potri.005G024800.1.v4.1 1035 811.436 2808 55.1431 Potri.004G059700.1.v4.1 961 737.441 40 0.864332 Potri.007G009000.2.v4.1 1416 1192.44 0 0 Potri.003G141000.2.v4.1 2943 2719.44 1325 7.76399 Potri.016G087400.1.v4.1 270 82.6069 5590.29 1078.37 Potri.015G069301.1.v4.1 564 342.779 0 0 Potri.010G195200.1.v4.1 1773 1549.44 1194.3 12.2825 Potri.012G127500.1.v4.1 977 753.441 12840 271.559 ==> SRR26075339.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 367 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 1475 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 66 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 2457 SRR26075339 completed mapping pipeline successfully