Starting /dee2/code/volunteer_pipeline.sh SRR26075340 current disk space = 3052907683840 free memory = 1425558868 SRR26075340 SRAfilesize b65be8aa830f28195e78170da5e9d7a1 SRR26075340.sra SRR26075340.sra file validated SRR26075340 is paired end SRR26075340 is conventional basespace SRR26075340 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075340_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.56825 37.0 37.0 37.0 37.0 37.0 2 36.622 37.0 37.0 37.0 37.0 37.0 3 36.647 37.0 37.0 37.0 37.0 37.0 4 36.62 37.0 37.0 37.0 37.0 37.0 5 36.6555 37.0 37.0 37.0 37.0 37.0 6 36.563 37.0 37.0 37.0 37.0 37.0 7 36.6105 37.0 37.0 37.0 37.0 37.0 8 36.691 37.0 37.0 37.0 37.0 37.0 9 36.6885 37.0 37.0 37.0 37.0 37.0 10-14 36.661500000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.55929999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.527 37.0 37.0 37.0 37.0 37.0 25-29 36.4423 37.0 37.0 37.0 37.0 37.0 30-34 36.3706 37.0 37.0 37.0 37.0 37.0 35-39 36.3043 37.0 37.0 37.0 37.0 37.0 40-44 36.243399999999994 37.0 37.0 37.0 37.0 37.0 45-49 36.1647 37.0 37.0 37.0 37.0 37.0 50-54 36.1657 37.0 37.0 37.0 37.0 37.0 55-59 36.0976 37.0 37.0 37.0 37.0 37.0 60-64 35.995400000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.924099999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.9622 37.0 37.0 37.0 37.0 37.0 75-79 35.919799999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.9057 37.0 37.0 37.0 37.0 37.0 85-89 35.812 37.0 37.0 37.0 37.0 37.0 90-94 35.7663 37.0 37.0 37.0 37.0 37.0 95-99 35.791000000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.73780000000001 37.0 37.0 37.0 37.0 37.0 105-109 35.7024 37.0 37.0 37.0 37.0 37.0 110-114 35.581 37.0 37.0 37.0 37.0 37.0 115-119 35.48989999999999 37.0 37.0 37.0 34.6 37.0 120-124 35.50320000000001 37.0 37.0 37.0 37.0 37.0 125-129 35.275099999999995 37.0 37.0 37.0 34.6 37.0 130-134 35.313100000000006 37.0 37.0 37.0 32.2 37.0 135-139 35.121 37.0 37.0 37.0 27.4 37.0 140-144 35.0051 37.0 37.0 37.0 25.0 37.0 145-149 35.0294 37.0 37.0 37.0 25.0 37.0 150-151 34.90925 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 7.0 22 7.0 23 3.0 24 12.0 25 9.0 26 7.0 27 12.0 28 21.0 29 35.0 30 33.0 31 50.0 32 74.0 33 97.0 34 165.0 35 412.0 36 2857.0 37 199.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.77694046721929 13.28811856317508 6.2547098718914835 32.68023109771414 2 20.4 13.275 33.95 32.375 3 17.9 18.224999999999998 31.825 32.05 4 22.225 25.575 25.575 26.625 5 24.6 30.45 24.8 20.150000000000002 6 23.425 33.275 24.75 18.55 7 14.95 25.8 41.075 18.175 8 19.7 25.95 32.324999999999996 22.025 9 18.675 23.05 35.3 22.975 10-14 20.625 28.444999999999997 28.08 22.85 15-19 20.19 27.255000000000003 28.345 24.21 20-24 20.635 27.41 28.525 23.43 25-29 20.95 27.73 27.229999999999997 24.09 30-34 21.3 28.18 27.089999999999996 23.43 35-39 21.26 27.045 28.185 23.51 40-44 20.474999999999998 27.779999999999998 27.3 24.445 45-49 20.66 28.299999999999997 27.325 23.715 50-54 21.165 27.16 27.48 24.195 55-59 20.895 26.900000000000002 28.249999999999996 23.955000000000002 60-64 20.89 27.405 27.55 24.154999999999998 65-69 20.9 26.900000000000002 28.58 23.62 70-74 22.025 26.834999999999997 28.15 22.99 75-79 21.015 27.165 27.395000000000003 24.425 80-84 21.865000000000002 27.43 27.565 23.14 85-89 21.16 27.450000000000003 27.395000000000003 23.995 90-94 22.14 27.05 26.85 23.96 95-99 21.52 26.61 28.555000000000003 23.315 100-104 21.875 27.21 27.455000000000002 23.46 105-109 21.099999999999998 27.744999999999997 27.295 23.86 110-114 21.695 27.169999999999998 26.715 24.42 115-119 21.775 27.034999999999997 27.284999999999997 23.905 120-124 21.709999999999997 26.46 27.26 24.57 125-129 22.46 26.88 26.745 23.915 130-134 21.505 27.689999999999998 27.26 23.544999999999998 135-139 22.509999999999998 26.384999999999998 27.560000000000002 23.544999999999998 140-144 22.465 27.195000000000004 26.790000000000003 23.549999999999997 145-149 22.720000000000002 27.32 26.040000000000003 23.919999999999998 150-151 22.825 26.424999999999997 25.775 24.975 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 1.0 13 1.5 14 0.5 15 0.0 16 0.5 17 0.5 18 0.5 19 1.5 20 1.0 21 0.0 22 0.5 23 1.0 24 2.5 25 3.5 26 2.0 27 4.5 28 8.0 29 12.0 30 16.0 31 15.5 32 20.0 33 27.0 34 37.5 35 54.5 36 72.5 37 96.0 38 110.5 39 121.0 40 161.0 41 179.0 42 193.5 43 255.0 44 298.5 45 302.0 46 296.0 47 279.0 48 244.0 49 209.0 50 187.0 51 159.5 52 125.0 53 98.0 54 74.0 55 59.5 56 42.5 57 36.5 58 43.5 59 34.5 60 20.0 61 14.0 62 12.0 63 9.0 64 8.0 65 6.5 66 4.0 67 9.0 68 9.5 69 5.5 70 5.0 71 3.0 72 3.0 73 2.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.475 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.775000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 66.2485299882399 42.25 2 20.031360250882006 25.55 3 8.232065856526852 15.75 4 3.2928263426107405 8.4 5 1.058408467267738 3.375 6 0.8232065856526851 3.15 7 0.1960015680125441 0.8750000000000001 8 0.03920031360250882 0.2 9 0.07840062720501764 0.44999999999999996 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTTGTTTGAAGCAGCAACATCGAAGTTGTGGGGGTGGGGGGGATCGCAG 9 0.22499999999999998 No Hit GGAGGATGTGATGTACTTTTTGCAGGATCAGCAAGCTTCTGCTGCCCATC 9 0.22499999999999998 No Hit CTCTCTTGAACTTAAAAACCTCCTTATTTTCTCTCTTTCCTCTACCTGAC 8 0.2 No Hit CATACTTCTGTGTCCAGCTGCGAGCAGTGGCTTCATATTTGGATCGGTCA 7 0.17500000000000002 No Hit GGTGGGTTTATGATTTGAGCAAGGATGAGTGGACTGAGTTAGCTCAAATG 7 0.17500000000000002 No Hit GGGTCTTTCATCTGCAACTCTTTTAGAGAAGTCAGTTAAAGGGAAACTAC 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGGTATCTCGTAT 7 0.17500000000000002 TruSeq Adapter, Index 3 (97% over 37bp) CCATCTCATATTTATATGTTGGTGCAAAATTCAATGTACCCTCCGACCAT 7 0.17500000000000002 No Hit TGTAAAGGTGGTTGTTTCAGTTTCCGTGATGTATGGGAAGTAATCTCTAA 6 0.15 No Hit CCCTTGCTGGCCATGTAGGTGTTGACTAATTGCATTTGCACATCCTGAGA 6 0.15 No Hit GCACAAATTCCAGCATTCAGCATCTAACGCCAGCACACACCCTCCAAAAT 6 0.15 No Hit CCCTACTTCAATATCTATTATCTGTCCAGCTTCAAGAAAATCCGCCATGT 6 0.15 No Hit GTCCTTGAGCGCTTCAGTGGCAGCATTATACCAGGATTTGGTGATGGGAA 6 0.15 No Hit GCCTCTTACACATGGTCCTTTAAAACTATGGCTCTGTGACAAACAAACCC 6 0.15 No Hit AGCTTGACCTTCTTCTTCTTGTGCTTGATCTTCTTGGGCTTAGTGTAGGT 6 0.15 No Hit ATTTCAGACACCAGCTTAGTATCAACCCCATGATCAACAATTTGAAAAAC 6 0.15 No Hit CTCCTAAACAGCATAGATGACATAACCCAACGTTTAAACCGACTTTTATT 6 0.15 No Hit GCTGATTTGACAAGGGCTAGTGACCTTTCATTTGATTTTTTAAGTTTAGC 6 0.15 No Hit GGGCTGCGACAATCGGCCGTATGCGGCGGTTGTTCTGTGATCTACTAGCG 6 0.15 No Hit GCTCTCCACTGATAGACACCACACTTCTGGCCTTCTCAATCAACTTATCG 6 0.15 No Hit AGCTGATAGTACGTAAAGACAAACGATGACCTTGAAGAAAAGGCGCTTGT 6 0.15 No Hit CTTTGCTCTTTAGGGCAGCTGCAAATGACCCTAGGTTAGCCTTCATGTCC 6 0.15 No Hit CTTTTCTTTAATTGTTGCTGTTAGTAAGTGTATCTCTTGCTCCAATTTTT 6 0.15 No Hit GCCATATTCTGATGGTTCCATCTTCGGATCCTGATGCATAAGATTCTCCT 6 0.15 No Hit GCTGAAAAGAAGAAGGAAATGGGGGCGCCGGCTGGAATGGGAAGAAGAAG 6 0.15 No Hit GTGCTGGCTTCCCTTCCTCGTCCAAAACTCTAATTGGGCCACTGAGATAG 6 0.15 No Hit CCAGGGTGGTTCATGATGATGACCTGAGAGGTGAAGTTGGCAGCCTCCTT 6 0.15 No Hit CCTTGATAGCGGCTTTAATTTCATCATATTTAGCTGACTTCTCAAGTCTT 6 0.15 No Hit GGAGCTTTTTGGCGACAAAAAGGGCGTCGGAGGCTGCTTGCTTTCCTTTA 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACATAGCGGTATCTCGTTT 5 0.125 TruSeq Adapter, Index 3 (97% over 37bp) AATCATCTTCACTTCAATTGCTGTTGCCAATGACTTCAGCTGACTTGGCG 5 0.125 No Hit GCTCAGTTTAACACGTGCCTAGTCAACACCCCAGTTGACGGTACTTGGTG 5 0.125 No Hit TACCATTATTAATATTGTTCATAGAACGATGATCAAGTCCAAGAAAATCA 5 0.125 No Hit GGCAAGTCACAGTACCTCTTGTAGAACCCAATACGATCCTCCACCTGTTG 5 0.125 No Hit CTCAAACATACAAAACTTAGCCAACCTAAATTACGAAATAAAGTCAGCAA 5 0.125 No Hit CCCCAAGCTGGCCTTCCAGTGATACTGGCAACCCATGCGCTAACATGGGG 5 0.125 No Hit GCTCTGTTCTGGTTGCAGAGGGATGCCAAACGGCCCTGCAGAGGTGAGTG 5 0.125 No Hit GTCACAACATATATATTTTGATCTAAACCCACTGTGCGCGCGCGCGCGAG 5 0.125 No Hit GGTTGGTGTGGTGCAAGGGACAGGTTCTCAGCGGACTGCTTGCATGACAA 5 0.125 No Hit CCAGGAAGAATATGATTTTATTAGGGGGTTTTATGTACAACCAGATGCGT 5 0.125 No Hit GCCATATACATATCGGCTATAGAGCAGCAACAAGCATTTCGATTCAGCAC 5 0.125 No Hit GTACCATTTCCAGCTCTGAAGCTGCCACCTTCTCTCATTACAACAAAGCC 5 0.125 No Hit GCTGCGTTGGCCATGATTTTCCTTCTTATAGGGATCGGAGGCAAAAATAA 5 0.125 No Hit GTGGCGAGGAGGCTGGTGCCCTTCTGGCAATGGCACTTGAAGTCGGTGAG 5 0.125 No Hit CTGGCATTGGGGTCAAAGACACTTCCAATGAAGCTGTACACTTGAGCAAA 5 0.125 No Hit GTAGCGATCATCTCTATGTAGTGCCAATGCATGACCGATGAAGTCTATGG 5 0.125 No Hit TGGCAAAAAGATCAGCTGTAAACTGGCATGAAAAGTGGTACTTTTCATCA 5 0.125 No Hit CGTCTGGATCGGGGATCGACCCCATGGTGGTGGCTGTTTCGATCAAGTTT 5 0.125 No Hit ACCGCTTGAAGTGATTCTCCTCCCACCCTTGGGATCAACATCGATGATGT 5 0.125 No Hit CAGAATTCCTGCTCCTCAGACAGCCACATCACAACAGTCTCAGTTAGCCT 5 0.125 No Hit GTCTAAGAAGAACATGCCTAACAGCTGCATCAATATATTCTTTAACAGGT 5 0.125 No Hit ATCTGCGATAGGCTAGTTCATAAACGAGGGGCGATGCCCGGGCTCAGAAT 5 0.125 No Hit GCTCAGGTTACATGATCAAGCAACGGAGATTCTGTAACTGCTTATCGCTC 5 0.125 No Hit CTTGAGCGCTTTAGTGGCAGCATTATACCAGGATGTGGTGATGGGAAGCC 5 0.125 No Hit GTCCACAGCTTCCTTCTCGAACCCAATCTCAGCATTAGAGTAGCCATCAC 5 0.125 No Hit GTGAGTGTTCTCCGCTTCACTGAGCTTAGCTAGTCCAAAGTCTGATATCT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.037500000000000006 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.175 0.0 0.0 0.0 0.0 88-89 0.1875 0.0 0.0 0.0 0.0 90-91 0.3 0.0 0.0 0.0 0.0 92-93 0.4 0.0 0.0 0.0 0.0 94-95 0.475 0.0 0.0 0.0 0.0 96-97 0.7124999999999999 0.0 0.0 0.0 0.0 98-99 0.8125 0.0 0.0 0.0 0.0 100-101 1.0875 0.0 0.0 0.0 0.0 102-103 1.2375 0.0 0.0 0.0 0.0 104-105 1.35 0.0 0.0 0.0 0.0 106-107 1.6 0.0 0.0 0.0 0.0 108-109 1.7875 0.0 0.0 0.0 0.0 110-111 2.125 0.0 0.0 0.0 0.0 112-113 2.2875 0.0 0.0 0.0 0.0 114-115 2.4 0.0 0.0 0.0 0.0 116-117 2.575 0.0 0.0 0.0 0.0 118-119 2.9625000000000004 0.0 0.0 0.0 0.0 120-121 3.575 0.0 0.0 0.0 0.0 122-123 3.9375 0.0 0.0 0.0 0.0 124-125 4.3125 0.0 0.0 0.0 0.0 126-127 4.8 0.0 0.0 0.0 0.0 128-129 5.1875 0.0 0.0 0.0 0.0 130-131 6.025 0.0 0.0 0.0 0.0 132-133 6.625 0.0 0.0 0.0 0.0 134-135 7.4625 0.0 0.0 0.0 0.0 136-137 8.2375 0.0 0.0 0.0 0.0 138-139 8.675 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAATTT 15 1.1411342E-4 145.0 145 CAGTGAA 10 0.006830828 145.0 145 CATACAA 15 1.1411342E-4 145.0 7 ATACAAA 15 1.1411342E-4 145.0 8 AACATAC 15 1.1411342E-4 145.0 5 TACAAAA 20 3.5877043E-4 108.75 9 AAACATA 25 8.7132835E-4 87.0 4 TCAAACA 25 8.7132835E-4 87.0 2 ACATACA 25 8.7132835E-4 87.0 6 CAAACAT 25 8.7132835E-4 87.0 3 CTCAAAC 25 8.7132835E-4 87.0 1 >>END_MODULE SRR26075340 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075340_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.94875 37.0 37.0 37.0 37.0 37.0 2 36.1045 37.0 37.0 37.0 37.0 37.0 3 36.193 37.0 37.0 37.0 37.0 37.0 4 36.1875 37.0 37.0 37.0 37.0 37.0 5 36.1235 37.0 37.0 37.0 37.0 37.0 6 36.0655 37.0 37.0 37.0 37.0 37.0 7 36.0605 37.0 37.0 37.0 37.0 37.0 8 36.1025 37.0 37.0 37.0 37.0 37.0 9 36.2185 37.0 37.0 37.0 37.0 37.0 10-14 36.1134 37.0 37.0 37.0 37.0 37.0 15-19 36.044200000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.0278 37.0 37.0 37.0 37.0 37.0 25-29 35.863800000000005 37.0 37.0 37.0 37.0 37.0 30-34 35.748599999999996 37.0 37.0 37.0 37.0 37.0 35-39 35.655 37.0 37.0 37.0 37.0 37.0 40-44 35.5889 37.0 37.0 37.0 37.0 37.0 45-49 35.5619 37.0 37.0 37.0 37.0 37.0 50-54 35.43300000000001 37.0 37.0 37.0 37.0 37.0 55-59 35.410199999999996 37.0 37.0 37.0 37.0 37.0 60-64 35.5042 37.0 37.0 37.0 37.0 37.0 65-69 35.4329 37.0 37.0 37.0 37.0 37.0 70-74 35.294 37.0 37.0 37.0 34.6 37.0 75-79 35.24249999999999 37.0 37.0 37.0 37.0 37.0 80-84 35.255700000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.2666 37.0 37.0 37.0 37.0 37.0 90-94 35.1699 37.0 37.0 37.0 29.8 37.0 95-99 35.2673 37.0 37.0 37.0 37.0 37.0 100-104 35.119 37.0 37.0 37.0 29.8 37.0 105-109 35.090500000000006 37.0 37.0 37.0 29.8 37.0 110-114 35.0973 37.0 37.0 37.0 29.8 37.0 115-119 35.0652 37.0 37.0 37.0 25.0 37.0 120-124 34.794900000000005 37.0 37.0 37.0 25.0 37.0 125-129 34.9355 37.0 37.0 37.0 25.0 37.0 130-134 34.8207 37.0 37.0 37.0 25.0 37.0 135-139 34.625600000000006 37.0 37.0 37.0 25.0 37.0 140-144 34.70005 37.0 37.0 37.0 25.0 37.0 145-149 34.67955 37.0 37.0 37.0 25.0 37.0 150-151 34.241125 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 3.0 13 5.0 14 3.0 15 11.0 16 10.0 17 8.0 18 4.0 19 11.0 20 8.0 21 10.0 22 9.0 23 7.0 24 14.0 25 14.0 26 18.0 27 14.0 28 16.0 29 23.0 30 30.0 31 49.0 32 67.0 33 91.0 34 242.0 35 833.0 36 2361.0 37 139.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.36209052263066 22.455613903475868 9.32733183295824 19.854963740935233 2 29.275000000000002 25.3 26.35 19.075 3 22.6 28.025 31.275 18.099999999999998 4 25.474999999999998 34.699999999999996 21.975 17.849999999999998 5 25.174999999999997 37.55 20.0 17.275 6 24.15 36.675000000000004 20.474999999999998 18.7 7 22.15 23.225 35.5 19.125 8 22.025 26.85 27.55 23.575 9 25.15 25.124999999999996 26.75 22.975 10-14 25.585 29.160000000000004 25.014999999999997 20.24 15-19 24.635 27.985 26.484999999999996 20.895 20-24 25.515 27.61 26.040000000000003 20.835 25-29 25.005 28.875 24.75 21.37 30-34 25.040000000000003 28.105000000000004 26.135 20.72 35-39 25.355 28.405 25.490000000000002 20.75 40-44 25.385 28.599999999999998 25.61 20.405 45-49 24.55 27.715 26.619999999999997 21.115000000000002 50-54 23.985 28.355000000000004 26.39 21.27 55-59 24.45 28.505000000000003 26.179999999999996 20.865000000000002 60-64 24.135 27.965 26.979999999999997 20.919999999999998 65-69 24.95 28.139999999999997 25.619999999999997 21.29 70-74 24.745 28.499999999999996 26.61 20.145 75-79 24.490000000000002 28.76 25.955000000000002 20.794999999999998 80-84 25.314999999999998 27.905 25.96 20.82 85-89 25.174999999999997 28.37 25.605 20.849999999999998 90-94 25.119999999999997 28.74 26.005 20.135 95-99 25.66 28.785 25.645 19.91 100-104 25.130000000000003 28.360000000000003 25.955000000000002 20.555 105-109 25.185000000000002 27.99 25.81 21.015 110-114 26.32 27.779999999999998 25.22 20.68 115-119 26.090000000000003 28.175 25.729999999999997 20.005 120-124 25.445 28.225 26.009999999999998 20.32 125-129 25.8 28.13 25.865 20.205000000000002 130-134 25.8 28.29 25.72 20.19 135-139 26.992699269926995 27.587758775877585 25.79257925792579 19.626962696269626 140-144 26.62932026209173 27.419596858900615 26.06912419346771 19.88195868553994 145-149 26.63731425426527 27.763045979886925 25.526592284985238 20.07304748086256 150-151 26.92259597349006 27.76041015380768 25.159434788045516 20.157559084656747 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.5 8 1.5 9 1.5 10 0.5 11 0.5 12 0.5 13 0.5 14 0.5 15 0.0 16 0.0 17 1.0 18 1.0 19 1.0 20 1.5 21 1.0 22 0.5 23 1.0 24 2.5 25 3.0 26 3.0 27 2.0 28 3.5 29 5.0 30 3.5 31 10.0 32 15.0 33 18.0 34 31.5 35 44.5 36 67.0 37 97.0 38 117.5 39 150.5 40 175.5 41 196.0 42 249.0 43 262.0 44 266.5 45 274.5 46 267.5 47 260.0 48 220.5 49 204.5 50 186.5 51 150.0 52 117.0 53 105.5 54 91.0 55 68.5 56 62.5 57 42.5 58 32.5 59 31.0 60 24.5 61 15.0 62 10.5 63 11.0 64 7.5 65 4.0 66 1.0 67 1.0 68 1.5 69 2.5 70 2.5 71 0.5 72 1.5 73 3.0 74 3.5 75 3.0 76 2.5 77 1.5 78 0.0 79 0.5 80 1.5 81 1.5 82 2.0 83 2.0 84 2.0 85 4.0 86 2.5 87 2.5 88 3.5 89 3.0 90 5.0 91 3.5 92 1.5 93 1.0 94 0.0 95 0.5 96 0.5 97 1.0 98 1.5 99 1.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.01 140-144 0.034999999999999996 145-149 0.065 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.525 #Duplication Level Percentage of deduplicated Percentage of total 1 67.49321968229368 43.55 2 19.759783029833397 25.5 3 7.593955831073227 14.7 4 2.905850445563735 7.5 5 1.162340178225494 3.75 6 0.7361487795428129 2.85 7 0.19372336303758234 0.8750000000000001 8 0.03874467260751647 0.2 9 0.03874467260751647 0.22499999999999998 >10 0.07748934521503294 0.8500000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 23 0.575 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 11 0.27499999999999997 No Hit ATTTATTGAAGGACATCACTGTGAATCCGACAATGTTGATAAATATACTT 9 0.22499999999999998 No Hit TGGTAAAATAACAATAATAATGCCAATTAAAGCCGAAGGCACAGGCAGCA 8 0.2 No Hit AAAACTAAAAGATAGAGGGAGGGAGAGAGAAAATAAGGAGGTTTTTAAGT 7 0.17500000000000002 No Hit ACAACAACCACCTTTTGTCAACAGCCTCTCACCTTTGGGTTTTGGTTACA 7 0.17500000000000002 No Hit GTCCTGCTCTTACCATCTCTAAGGTACTGCTCTCCATTTGCTCATTGCTG 7 0.17500000000000002 No Hit CTCGTCCCCGATCACCACTCCCTCACACTCATCTCGCTCTTGACTCATTT 7 0.17500000000000002 No Hit AGGACGGGGATGAACGCAAAAGAAATGCTGATGTTCATGAAATACATAGA 7 0.17500000000000002 No Hit GGAGCTGCAAAGGCTGTTGGGAAGGTGCTGCCTGCTCTGAATGGCAAGCT 6 0.15 No Hit CTTCAACCCCAAGACTAGTAGTGCCAATAGACTTGAAGAAGAAGCCTTGG 6 0.15 No Hit GCTATCTTGATGCATAATAAGGTTCTATCTGTTGTTGGGCTTCTTCAAGA 6 0.15 No Hit GTCATCGTCACCTTCCAAACCCAACAACAGGTCTTTTTGAGGGACTGAGT 6 0.15 No Hit GACCACTGAAGTTAAATCTGTTGAGATGCACCATGAAGCTCTTCAGGAGG 6 0.15 No Hit GTACTACTGGTGCTGCTTACCTGTCATCGACCATGGCTTCGCAGTCACGA 6 0.15 No Hit GTTAAATTTTGGGATGCAAATCACTTTGGATTGGTGAAAAGCTATGACAT 6 0.15 No Hit AAACAGTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCT 6 0.15 No Hit GGCAAGACAATAACCCTCGAGGTAGAATCCTCAGATACAATCGACAATGT 6 0.15 No Hit GTCTTTTGTTTCAAGGCAGAGGCAGTTCGGCGAAGGTGCTCATGTTGAGA 6 0.15 No Hit GTTTGAAAAGGACACGGAAGTTTGGCAGCACAGGGTTGAGAATTATTGGA 6 0.15 No Hit GTGATGTTTCTGTGCCGCAGGCTGTTGCCTTCACAGGAGTTTGGAACTGG 6 0.15 No Hit AACAAAAAAAAGAAGAAAAAAACCTAACCCTACAAAACTTAATCTTCTTC 6 0.15 No Hit CTTGGTTTACTACCACTGTGCCTATCGGGATTCTAGCCTCTTCTCTTTGA 6 0.15 No Hit GTAGCGGGTGACATTAACAAGAGGAAAGGAGTGATTGTTGGAAATGACCA 6 0.15 No Hit ATCAAACTGGAATGGAATAAACGCAAGACTGTGAACTATTGAGGCTCATA 6 0.15 No Hit GAAGAGTAATGCTGTTGAAATCAGAGAATTGATTATCAGATGTGTCTCAC 6 0.15 No Hit AGAGCCACAAAGTCTATCACCTCTAACATCCACCGTCTCATCTCCAAAGG 6 0.15 No Hit GCTCAAAATGTGAAACCTGCTAGCAAGTTGGAAATTGAAGATTCAGATAT 6 0.15 No Hit TTACAATTTACTTCACTTAAAGAGGAGTGGGAAATCATTTCAGAAGAACA 5 0.125 No Hit ATTTCTTTTCCTCAAGTTCTTAAAGCTCACTCTTTTCTGTCCGTCCGATC 5 0.125 No Hit CTTCACAGGAGTTTGGGAATGGAAGAAATTCCGATCGGAGGAAGGTAAAC 5 0.125 No Hit GCTTCTTCTTCCTCTTCTTCTGCTATGGACACAACGACAATTACCACCAC 5 0.125 No Hit GGTCCGGAAATGGATCTCTCGCTTTGAAGTTTGGCCATACCTAGAAACTT 5 0.125 No Hit CTGTGCCATTGCTCAGGCTGAGTCTCTTCGTTACAAGCTTCTCGGAGGCC 5 0.125 No Hit GGATCACCAGCCTCTCTTTGGTGGGCGGACCGCCATTGAGATGTATAGTG 5 0.125 No Hit CCAAAATCAAGCCGAAAACGATGGCCCCTTCTACCCTCACAGCTCTGGCA 5 0.125 No Hit GGAACATTGAATTTCTTGTTTAAATTTGGCGAGAAATTCAATGTGTCTGC 5 0.125 No Hit GCTTTCCGTAGGATTTATGGTGGGAGCCAAAGGAATGGAAGTCGTCCACC 5 0.125 No Hit TGATACCAGATTTGATATTCGAAGCTCATCCGATGCTTCAACTCTATGAT 5 0.125 No Hit GATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGATTGTCG 5 0.125 No Hit TCTAGCTAACTTTATCCCCCCCTCTCTCTCTTCCCTTGATCTTTCTCTCT 5 0.125 No Hit TGATGCCAAAAATACCGCTCGTCTGCTTGCTCTACTTATGCGCCGGGGCA 5 0.125 No Hit GCTTTGTCTCTTGGATTTTGGCCTGACAATTCAGGGCCACAAGCCAGAAG 5 0.125 No Hit GATATCTCTATCGGCCAGATACAACATCAGATCTTGGAAATCCAATTACC 5 0.125 No Hit TCTAAATATCCGGAACTCAAAAGACCAGAACTTTCGCTACGTCGATCTAA 5 0.125 No Hit AAGAGGGAGAGAGACATAGGGGAGGACCTCCATTATCTCTCAAAAAGTAA 5 0.125 No Hit GTTGGACAGCAAGGTATCACAAGTACACGCTTTACGAGCACAGACAGGTC 5 0.125 No Hit CAATAATCCAAAGTCTGATGCGAGGAAAGACAGGAAATCAGGGACTGGAA 5 0.125 No Hit TCGCATTTTGAAAATTCTATGGAAGAGCTAGCATCTCTGACGAAAACAGC 5 0.125 No Hit CTCCCACTATTTTCACTGCTAATCATCATCACAACAAAGATACAGAGAAA 5 0.125 No Hit TTCTCTTTCTCTGAAGAACTGGACAGACTAAAACATGTGTGGAGGTGCCA 5 0.125 No Hit ACCAACAGATGCAGCTCTAGTTGCCGAGACGGAGGTGACTCTCGGTAAGG 5 0.125 No Hit GCTTCGGCAGACAGCCGCCTTGAAGTTGATGTTGGATGGGAGGAAATGGA 5 0.125 No Hit GAGAGAGGTTAATAAGGCCTAGCCTAAAGGTTCTTGCAGAGCAACATCAT 5 0.125 No Hit AGTATCTGTACTTCAAAGCAGTGGATGGCGGCTACGTCTTCAACAAGGGA 5 0.125 No Hit ATTTGGTTTTGGATGACAGCACAGTCTCCAAAGCCCTCATGCCACAGTGT 5 0.125 No Hit CTGGTGGTGAGTTGTCTCCAGCTTCTTCAAAGAGCACGCAAGTGCAATCC 5 0.125 No Hit CCAAGTGGAAGGAGAAGAGAGGGAAAAAGAAGAGAAGCATGAGCATGAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.0625 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.0875 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.175 0.0 0.0 0.0 0.0 84-85 0.2 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.21250000000000002 0.0 0.0 0.0 0.0 90-91 0.325 0.0 0.0 0.0 0.0 92-93 0.42500000000000004 0.0 0.0 0.0 0.0 94-95 0.5 0.0 0.0 0.0 0.0 96-97 0.7875000000000001 0.0 0.0 0.0 0.0 98-99 0.8875 0.0 0.0 0.0 0.0 100-101 1.1375 0.0 0.0 0.0 0.0 102-103 1.275 0.0 0.0 0.0 0.0 104-105 1.375 0.0 0.0 0.0 0.0 106-107 1.625 0.0 0.0 0.0 0.0 108-109 1.8125 0.0 0.0 0.0 0.0 110-111 2.1500000000000004 0.0 0.0 0.0 0.0 112-113 2.3125 0.0 0.0 0.0 0.0 114-115 2.425 0.0 0.0 0.0 0.0 116-117 2.625 0.0 0.0 0.0 0.0 118-119 3.0374999999999996 0.0 0.0 0.0 0.0 120-121 3.6375 0.0 0.0 0.0 0.0 122-123 3.9875 0.0 0.0 0.0 0.0 124-125 4.3375 0.0 0.0 0.0 0.0 126-127 4.825 0.0 0.0 0.0 0.0 128-129 5.2 0.0 0.0 0.0 0.0 130-131 6.0 0.0 0.0 0.0 0.0 132-133 6.625 0.0 0.0 0.0 0.0 134-135 7.525 0.0 0.0 0.0 0.0 136-137 8.3 0.0 0.0 0.0 0.0 138-139 8.774999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTACCA 10 0.006830828 145.0 9 CTAAAAA 20 3.5877043E-4 108.75 145 TTCCTCT 25 8.7132835E-4 87.0 9 GCTTCTT 25 8.7132835E-4 87.0 1 CTTCCTC 25 8.7132835E-4 87.0 8 TTCTTCC 35 0.0033124194 62.14286 6 TCTTCCT 35 0.0033124194 62.14286 7 >>END_MODULE Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110999 spots for SRR26075340.sra Written 3110999 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra Read 3110980 spots for SRR26075340.sra Written 3110980 spots for SRR26075340.sra SRR ids: ['SRR26075340.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_y02jpaan SRR26075340.sra spots: 62219619 blocks: [[1, 3110980], [3110981, 6221960], [6221961, 9332940], [9332941, 12443920], [12443921, 15554900], [15554901, 18665880], [18665881, 21776860], [21776861, 24887840], [24887841, 27998820], [27998821, 31109800], [31109801, 34220780], [34220781, 37331760], [37331761, 40442740], [40442741, 43553720], [43553721, 46664700], [46664701, 49775680], [49775681, 52886660], [52886661, 55997640], [55997641, 59108620], [59108621, 62219619]] SRR26075340 file size 22985210 SRR26075340 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075340 SRR26075340_1.fastq SRR26075340_2.fastq Input file: SRR26075340_1.fastq Paired file: SRR26075340_2.fastq trimmed: SRR26075340-trimmed-pair1.fastq, SRR26075340-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 20:48:42 2025 >> started Tue Feb 11 20:49:56 2025 >> done (73.345s) 62219619 read pairs processed; of these: 395 ( 0.00%) short read pairs filtered out after trimming by size control 265602 ( 0.43%) empty read pairs filtered out after trimming by size control 61953622 (99.57%) read pairs available; of these: 9058581 (14.62%) trimmed read pairs available after processing 52895041 (85.38%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 32 0.00% 19 55 0.00% 20 54 0.00% 21 57 0.00% 22 72 0.00% 23 101 0.00% 24 125 0.00% 25 118 0.00% 26 131 0.00% 27 100 0.00% 28 122 0.00% 29 146 0.00% 30 136 0.00% 31 115 0.00% 32 163 0.00% 33 129 0.00% 34 169 0.00% 35 131 0.00% 36 203 0.00% 37 170 0.00% 38 218 0.00% 39 167 0.00% 40 233 0.00% 41 208 0.00% 42 233 0.00% 43 210 0.00% 44 238 0.00% 45 265 0.00% 46 284 0.00% 47 308 0.00% 48 310 0.00% 49 344 0.00% 50 420 0.00% 51 393 0.00% 52 517 0.00% 53 518 0.00% 54 494 0.00% 55 623 0.00% 56 617 0.00% 57 658 0.00% 58 767 0.00% 59 794 0.00% 60 937 0.00% 61 892 0.00% 62 1024 0.00% 63 1201 0.00% 64 1179 0.00% 65 1211 0.00% 66 1338 0.00% 67 1459 0.00% 68 1630 0.00% 69 1761 0.00% 70 2112 0.00% 71 2240 0.00% 72 2538 0.00% 73 2708 0.00% 74 3008 0.00% 75 3341 0.01% 76 3616 0.01% 77 3634 0.01% 78 4074 0.01% 79 4456 0.01% 80 4913 0.01% 81 5652 0.01% 82 6300 0.01% 83 7083 0.01% 84 7714 0.01% 85 8771 0.01% 86 9123 0.01% 87 10065 0.02% 88 10624 0.02% 89 12152 0.02% 90 12831 0.02% 91 14108 0.02% 92 15873 0.03% 93 18191 0.03% 94 20152 0.03% 95 21576 0.03% 96 23823 0.04% 97 25390 0.04% 98 27387 0.04% 99 30269 0.05% 100 32247 0.05% 101 35744 0.06% 102 39531 0.06% 103 42932 0.07% 104 47549 0.08% 105 52293 0.08% 106 57129 0.09% 107 59399 0.10% 108 63825 0.10% 109 66897 0.11% 110 70054 0.11% 111 77264 0.12% 112 82575 0.13% 113 90114 0.15% 114 97454 0.16% 115 104462 0.17% 116 110074 0.18% 117 115968 0.19% 118 119488 0.19% 119 124597 0.20% 120 130186 0.21% 121 137546 0.22% 122 144651 0.23% 123 154360 0.25% 124 165962 0.27% 125 173495 0.28% 126 181920 0.29% 127 186928 0.30% 128 191587 0.31% 129 196533 0.32% 130 201491 0.33% 131 206659 0.33% 132 214749 0.35% 133 225201 0.36% 134 230400 0.37% 135 241450 0.39% 136 244983 0.40% 137 253394 0.41% 138 258960 0.42% 139 260315 0.42% 140 263558 0.43% 141 269209 0.43% 142 274997 0.44% 143 283623 0.46% 144 292881 0.47% 145 298010 0.48% 146 301721 0.49% 147 306869 0.50% 148 309149 0.50% 149 307527 0.50% 150 313167 0.51% 151 52895041 85.38% 61953622 reads passed initial QC criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=3.51 fanout-score-rank=20 prefix-density=0.39 prefix-fanout=2.9 sequence=TGGTGATGGGAAGCCAGAAAACTTCCTTGGGCGCTTCACTTGGAGAGAACATGTTAATTTTCTCATACTGTACAGTCCCCA criterion=fanout-score sequence-density=0.03 sequence-density-rank=33 fanout-score=49.62 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=9.1 sequence=TTCACCATTTTACTTCACCAATTCCTTAGAGATGTAATAGCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAGGAAGAAATATGACAAGGAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATGGAGCTATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATTATTTTTAGAAATCCCATACTGCCACCGAATATTCAGTCCTTTAAGAAATCGAACAGCATACCCAACATAGTAAAAACCATCAATAATGCAAATACCGTTACCACAAGTGCAAATACTCCCATTCCTACCTCTCC criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=2.94 fanout-score-rank=21 prefix-density=0.33 prefix-fanout=2.9 sequence=ATGTACCCTGACTTAGGTTTCTCAGA criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=225.98 fanout-score-rank=1 prefix-density=0.15 prefix-fanout=11.1 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTA SRR26075340 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 20:52:22 Started mapping on | Feb 11 20:52:22 Finished on | Feb 11 21:04:45 Mapping speed, Million of reads per hour | 300.18 Number of input reads | 61953622 Average input read length | 288 UNIQUE READS: Uniquely mapped reads number | 46731766 Uniquely mapped reads % | 75.43% Average mapped length | 287.26 Number of splices: Total | 45123229 Number of splices: Annotated (sjdb) | 44026557 Number of splices: GT/AG | 44249472 Number of splices: GC/AG | 640567 Number of splices: AT/AC | 44846 Number of splices: Non-canonical | 188344 Mismatch rate per base, % | 0.47% Deletion rate per base | 0.04% Deletion average length | 3.32 Insertion rate per base | 0.02% Insertion average length | 2.47 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1491338 % of reads mapped to multiple loci | 2.41% Number of reads mapped to too many loci | 288282 % of reads mapped to too many loci | 0.47% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 21.26% % of reads unmapped: other | 0.44% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 13730528 13730528 13730528 N_multimapping 1491338 1491338 1491338 N_noFeature 1186547 46269313 1433140 N_ambiguous 1005423 10151 781606 UnstrandedReadsAssigned:44539796 PositiveStrandReadsAssigned:452302 NegativeStrandReadsAssigned:44517020 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075340 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075340-trimmed-pair1.fastq SRR26075340-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 61,953,622 reads, 51,897,698 reads pseudoaligned [quant] estimated average fragment length: 203.476 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,189 rounds 52401 SRR26075340.ke.tsv 34699 SRR26075340.se.tsv 87100 total ==> SRR26075340.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1815.52 6182 59.9343 Potri.005G024800.1.v4.1 1035 832.524 3260 68.9238 Potri.004G059700.1.v4.1 961 758.536 4 0.092818 Potri.007G009000.2.v4.1 1416 1213.52 0 0 Potri.003G141000.2.v4.1 2943 2740.52 1770.34 11.3703 Potri.016G087400.1.v4.1 270 94.2279 5013.99 936.596 Potri.015G069301.1.v4.1 564 362.893 0 0 Potri.010G195200.1.v4.1 1773 1570.52 1415 15.8584 Potri.012G127500.1.v4.1 977 774.536 31329 711.956 ==> SRR26075340.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 28 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 917 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 15 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1770 SRR26075340 completed mapping pipeline successfully