Starting /dee2/code/volunteer_pipeline.sh SRR26075341 current disk space = 3052588306432 free memory = 1573513896 SRR26075341 SRAfilesize 6e24e74904288aee242202a2a21acd4c SRR26075341.sra SRR26075341.sra file validated SRR26075341 is paired end SRR26075341 is conventional basespace SRR26075341 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075341_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.426 37.0 37.0 37.0 37.0 37.0 2 36.6115 37.0 37.0 37.0 37.0 37.0 3 36.5995 37.0 37.0 37.0 37.0 37.0 4 36.6525 37.0 37.0 37.0 37.0 37.0 5 36.7555 37.0 37.0 37.0 37.0 37.0 6 36.6875 37.0 37.0 37.0 37.0 37.0 7 36.708 37.0 37.0 37.0 37.0 37.0 8 36.7705 37.0 37.0 37.0 37.0 37.0 9 36.6635 37.0 37.0 37.0 37.0 37.0 10-14 36.6709 37.0 37.0 37.0 37.0 37.0 15-19 36.597500000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.6134 37.0 37.0 37.0 37.0 37.0 25-29 36.5577 37.0 37.0 37.0 37.0 37.0 30-34 36.4413 37.0 37.0 37.0 37.0 37.0 35-39 36.427800000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.4225 37.0 37.0 37.0 37.0 37.0 45-49 36.3032 37.0 37.0 37.0 37.0 37.0 50-54 36.3328 37.0 37.0 37.0 37.0 37.0 55-59 36.2325 37.0 37.0 37.0 37.0 37.0 60-64 36.155 37.0 37.0 37.0 37.0 37.0 65-69 36.0512 37.0 37.0 37.0 37.0 37.0 70-74 36.1406 37.0 37.0 37.0 37.0 37.0 75-79 36.1376 37.0 37.0 37.0 37.0 37.0 80-84 36.0734 37.0 37.0 37.0 37.0 37.0 85-89 36.0287 37.0 37.0 37.0 37.0 37.0 90-94 35.899899999999995 37.0 37.0 37.0 37.0 37.0 95-99 35.9282 37.0 37.0 37.0 37.0 37.0 100-104 35.9217 37.0 37.0 37.0 37.0 37.0 105-109 35.814299999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.680699999999995 37.0 37.0 37.0 37.0 37.0 115-119 35.6323 37.0 37.0 37.0 37.0 37.0 120-124 35.6171 37.0 37.0 37.0 37.0 37.0 125-129 35.3955 37.0 37.0 37.0 34.6 37.0 130-134 35.344 37.0 37.0 37.0 34.6 37.0 135-139 35.0866 37.0 37.0 37.0 29.8 37.0 140-144 34.9365 37.0 37.0 37.0 25.0 37.0 145-149 35.0097 37.0 37.0 37.0 27.4 37.0 150-151 34.710750000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 0.0 22 2.0 23 3.0 24 6.0 25 4.0 26 14.0 27 7.0 28 20.0 29 20.0 30 31.0 31 37.0 32 63.0 33 104.0 34 172.0 35 469.0 36 2853.0 37 194.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.07329317269076 14.457831325301203 8.258032128514056 40.21084337349398 2 18.675 14.174999999999999 36.3 30.85 3 17.7 17.5 27.775 37.025000000000006 4 21.975 21.75 25.074999999999996 31.2 5 23.75 27.55 27.075 21.625 6 24.625 29.45 23.799999999999997 22.125 7 16.725 28.299999999999997 39.475 15.5 8 18.55 26.174999999999997 30.575000000000003 24.7 9 20.325 24.625 31.900000000000002 23.150000000000002 10-14 20.13201320132013 28.942894289428946 28.147814781478147 22.777277727772777 15-19 20.78 27.779999999999998 27.505000000000003 23.935000000000002 20-24 20.755000000000003 27.975 27.505000000000003 23.765 25-29 19.869999999999997 28.410000000000004 27.665 24.055 30-34 20.18 27.74 27.46 24.62 35-39 20.62 26.240000000000002 28.425 24.715 40-44 21.085 27.22 27.465 24.23 45-49 20.48 26.75 28.105000000000004 24.665 50-54 20.380000000000003 27.675 27.515 24.43 55-59 20.29 27.48 27.605 24.625 60-64 20.674999999999997 27.065 28.165000000000003 24.095 65-69 20.735 27.195000000000004 27.810000000000002 24.26 70-74 21.935 26.91 27.389999999999997 23.765 75-79 20.315 26.775 27.975 24.935 80-84 21.6 27.87 26.125 24.404999999999998 85-89 21.535 27.96 26.185000000000002 24.32 90-94 20.974999999999998 27.325 26.82 24.88 95-99 20.74 26.919999999999998 28.249999999999996 24.09 100-104 21.285 27.565 27.11 24.04 105-109 21.595 27.67 26.63 24.104999999999997 110-114 22.475 28.194999999999997 26.38 22.95 115-119 21.325 27.139999999999997 27.095000000000002 24.44 120-124 21.955 27.655 26.865 23.525 125-129 21.805 27.72 26.085 24.39 130-134 22.065 26.815 26.619999999999997 24.5 135-139 22.915 27.04 26.08 23.965 140-144 23.06 27.52 25.979999999999997 23.44 145-149 22.18 27.315 26.465 24.04 150-151 22.5 27.750000000000004 26.4125 23.3375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 1.0 14 0.5 15 0.0 16 0.5 17 0.5 18 0.0 19 0.5 20 1.0 21 1.0 22 1.5 23 1.0 24 0.0 25 2.5 26 5.5 27 5.5 28 7.5 29 13.5 30 18.0 31 24.5 32 28.0 33 25.0 34 33.5 35 42.5 36 54.5 37 86.0 38 115.5 39 134.0 40 153.0 41 178.0 42 218.5 43 228.0 44 254.0 45 300.5 46 290.0 47 266.5 48 247.0 49 210.0 50 183.5 51 161.5 52 134.0 53 108.0 54 86.0 55 76.0 56 67.0 57 55.5 58 31.0 59 29.0 60 27.0 61 14.5 62 14.5 63 11.0 64 6.0 65 5.0 66 6.5 67 7.5 68 8.5 69 8.0 70 3.5 71 2.5 72 3.0 73 1.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.01 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.800000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 66.57523510971787 42.475 2 19.74921630094044 25.2 3 8.22884012539185 15.75 4 3.2523510971786838 8.3 5 0.9796238244514107 3.125 6 0.7053291536050157 2.7 7 0.35266457680250785 1.575 8 0.03918495297805642 0.2 9 0.11755485893416928 0.675 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACCCGTATGCTTTGTCACAATCAACAGCTGGAAGCGCTAACAAATCAGTG 9 0.22499999999999998 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTTGAGTATCTCGTAT 9 0.22499999999999998 TruSeq Adapter, Index 2 (97% over 38bp) CTCTGGAATCCACTTCTTAGAAACATTTTCGTAACTGGCCTTGCTAATGA 9 0.22499999999999998 No Hit GGTCCATCCTCTAGCTTCGGGTTCTCTGGAACTAACCGGTGGTTTCTTCA 8 0.2 No Hit CCTCTTAATTCGTGAAAGGTGGATCGCGCCAAAGCACATGGGGCAGGGCT 7 0.17500000000000002 No Hit CATAGTTATTACGTACGATACGGAATGCCCCGCAGATCGTGCAGATGCTG 7 0.17500000000000002 No Hit TGGTAATGGTCCGGCCACTTGGGCCAATCGCTACAGCTGCAGGAATGCCT 7 0.17500000000000002 No Hit GGCTTGCTGGGTAGAGGTTTTAGCATGATTTGGCAGCGATTTAGGGGCTG 7 0.17500000000000002 No Hit GGCCTATTCATTCCTGCTAACAGTATGACCCAATCACGCTCCACAGCAAC 7 0.17500000000000002 No Hit GCGCGAGGATCCCTCCAGCTTAGTAGCGCTTGAACCCGCTCTCCTTGGGT 7 0.17500000000000002 No Hit AGCAAGGAAGGCGTTGTAGGAGGCTAAGTAGCTAACTCCTGAGTCTGAAC 7 0.17500000000000002 No Hit GTAGAGGCAGAGGAATGAAAGAGTACAAAAGGGTCATTTTTGGCTTGTCC 7 0.17500000000000002 No Hit GCATCATGCCGGCACTTGTATGCACATGGAAGACTGCGCACCTCATCCCT 7 0.17500000000000002 No Hit GGCAGCTAACGATGCTCCACTTCCCGAACCGTGTCTAGCGAGAATAACCA 6 0.15 No Hit CTGGTCTTGTCAGGAACAATGTGGATGAAAAGCTCAGGCTGAGCATCGAG 6 0.15 No Hit GATGAGGGCTTCTCAGGGGAATTTTTGAAGACCAATTCAGTGCAACGCTT 6 0.15 No Hit CAGTGGGCAATCTCTACAGTAAAAAAGAAATTGTCTTTGAGGATTCCGAG 6 0.15 No Hit CTCTTACAGACCCTATTAATCATATCACTCGAGCCTAAACCCCATTGCTC 6 0.15 No Hit CCCGCAACGACATCGGAATCCGGTCAACCCTACCCTTCGTCGGCACCCTG 6 0.15 No Hit GATGAGGATGGTGGTGGGGATGGTGGCGCAGGAGATAATGGGGTTATTTT 6 0.15 No Hit GAGCTCCGGCGGTATCATTTTGGCCGTCGCCTGGATACCAGAAAGAGTGA 6 0.15 No Hit CCGCAGACCCGAGCGAAAGCGGCGGTCCTTACAAGTCCGCTCCTCGGGGA 6 0.15 No Hit TGCCACACATATGAAAAACAAGTAAGAGACTGGCCATGACATGGTTGCTG 6 0.15 No Hit CTTGAATGTTTCAGTCCTTGGGTGGGACGAACTTTGCATATTGTTCAGCC 6 0.15 No Hit ATTTGATTGAAAATGGCAGGCATCTAGATAGATATGTATGACTTAATTGT 6 0.15 No Hit AGCGGGTGTATTTAGGACGCACGCAGCCCTATTACGAGGGTTGTAGTCGT 6 0.15 No Hit CTTACTGTTGTTCGAAAGAAAAACTTTACTCATGTGCTAGCCTTCTTGCC 6 0.15 No Hit GTGCATCACGCCTGCGCTTGAAAGAACAGCTATCAGTGGGAGCACTGCTA 6 0.15 No Hit GCAAATGTTATGATCCAACAAACTAGCAAACTAGCTAGCACATCGCACTA 6 0.15 No Hit GTGCTTGGATCCTGGCAGTGTTGATGAGGATGTTGGCATCAGACATAATT 6 0.15 No Hit CCGTGCTCAAAAGGGACACGAACATGGAGTCGCCCATGCAAGCTAACAAC 6 0.15 No Hit GTTCACACTGCTTCCCGGATACCCAGCATTCACATCCTTGTCCTCAGCAA 5 0.125 No Hit CTCAGAGAAATTCAAGAGAGAGATAAAGACTCACCCTTTCCCTTTCTCTC 5 0.125 No Hit GCAGGGGGGTAATCATTTGCAAGAATTTTATTCTTTTCTGAAGGGAGATA 5 0.125 No Hit CTAACTCTTTATTTCAAACCAGAACTTTCTTTTCCAATACCAAATTTGCC 5 0.125 No Hit TGGCAGGTCTTTTACGTGGATTGAACATTCCCCTGAGAGTGGGTATGGGA 5 0.125 No Hit GTCACTGCTGCTATGGCCATCATCATTCTTCTTCTTGTCCTCCTTCTATT 5 0.125 No Hit ATACGCAATCTTCTTATAAATCTCCCATGGCATATTTGAATCAACCAAAG 5 0.125 No Hit ACCGGATTGGTTCTAGAAGAGGATTGTAGGGTAAAACTCAAGCAAACAAA 5 0.125 No Hit GACCATAAGAACAGCCTATAAAACTGCATCAATTCCCAACTGTAACAACC 5 0.125 No Hit GTTTTGTTTTCAAGGGAGGGAGCGACGGCTTGAGCAGATCAGAGCTGAAA 5 0.125 No Hit GCTCTGCATACAATGGCCAAGCCACCATTGGCACACCAGCACACACTGCC 5 0.125 No Hit ATGAATTCCTCCAGGATGAAGTGAATTAGCAGTTATGTTTACCCCCTCTT 5 0.125 No Hit CCAAAGTCATAGTTTTTGTACTTCTCCAACATGGCAGACATCCCCTTGCT 5 0.125 No Hit GCTTAGCCTTTTTCTCCTGGATCATCTTCTCCCGATCCTCATAAAACACA 5 0.125 No Hit GGTTGCAAGAGTGCCTGGAGTGCCAAGAATTTCTCCATCACTAAAGAATG 5 0.125 No Hit TTTCACAATAGTAGTGGTATCTGGTGGTTGTGTGTGGTAAATTGCAGTGG 5 0.125 No Hit GCCAAGAAGGACAACTTTGAAAGCTTAAAAATAACAAAGCCAAAACCAAA 5 0.125 No Hit GCCATATCTTTTGGGGATGCAGCAGTTTCTTTGATCGGAACTGCAGAGGA 5 0.125 No Hit CATCATCTAAAGCCTTGTACTCGTAAACCACAAAATCGAAAAAAAAGCGC 5 0.125 No Hit CGCTTGGTATCTGGGCTCCTCTCGATTAAGTGGAGAGCGAGATCTGGAGA 5 0.125 No Hit GTAGAAATAGAGATAACATATCTCAAGTGAACATGATCCAGTTACCTGAA 5 0.125 No Hit GAGCTATCATATGTGTTAAAAGCAAAAGTTCTAGATGTAAATTGTAATTC 5 0.125 No Hit ATGGGGGACATATGTTGCGTGCTTAACTTGGACTCCAACATAGTCAGCAA 5 0.125 No Hit GGCTTAGCGAACCAGGTCATAGACTTAAAAGTAGCAAGCCTCCTCATAAG 5 0.125 No Hit TCTCGCACCTTCTAGGATCAGCAACAAGCAAAGTCCTATCATACCTCACC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.05 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.275 0.0 0.0 0.0 0.0 82-83 0.35 0.0 0.0 0.0 0.0 84-85 0.4625 0.0 0.0 0.0 0.0 86-87 0.65 0.0 0.0 0.0 0.0 88-89 0.9750000000000001 0.0 0.0 0.0 0.0 90-91 1.0875 0.0 0.0 0.0 0.0 92-93 1.2375 0.0 0.0 0.0 0.0 94-95 1.4375 0.0 0.0 0.0 0.0 96-97 1.6375 0.0 0.0 0.0 0.0 98-99 1.9500000000000002 0.0 0.0 0.0 0.0 100-101 2.2 0.0 0.0 0.0 0.0 102-103 2.575 0.0 0.0 0.0 0.0 104-105 2.8875 0.0 0.0 0.0 0.0 106-107 3.5625 0.0 0.0 0.0 0.0 108-109 4.0875 0.0 0.0 0.0 0.0 110-111 4.3875 0.0 0.0 0.0 0.0 112-113 4.8375 0.0 0.0 0.0 0.0 114-115 5.6125 0.0 0.0 0.0 0.0 116-117 6.275 0.0 0.0 0.0 0.0 118-119 6.7125 0.0 0.0 0.0 0.0 120-121 7.2125 0.0 0.0 0.0 0.0 122-123 7.9375 0.0 0.0 0.0 0.0 124-125 8.725000000000001 0.0 0.0 0.0 0.0 126-127 9.45 0.0 0.0 0.0 0.0 128-129 10.0 0.0 0.0 0.0 0.0 130-131 10.6125 0.0 0.0 0.0 0.0 132-133 11.2375 0.0 0.0 0.0 0.0 134-135 11.95 0.0 0.0 0.0 0.0 136-137 12.6625 0.0 0.0 0.0 0.0 138-139 13.3875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAATGT 10 0.006830828 145.0 8 >>END_MODULE SRR26075341 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075341_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.977 37.0 37.0 37.0 37.0 37.0 2 36.1175 37.0 37.0 37.0 37.0 37.0 3 36.134 37.0 37.0 37.0 37.0 37.0 4 36.19 37.0 37.0 37.0 37.0 37.0 5 36.225 37.0 37.0 37.0 37.0 37.0 6 36.112 37.0 37.0 37.0 37.0 37.0 7 36.0965 37.0 37.0 37.0 37.0 37.0 8 36.115 37.0 37.0 37.0 37.0 37.0 9 36.1995 37.0 37.0 37.0 37.0 37.0 10-14 36.124 37.0 37.0 37.0 37.0 37.0 15-19 36.1463 37.0 37.0 37.0 37.0 37.0 20-24 36.126 37.0 37.0 37.0 37.0 37.0 25-29 35.982 37.0 37.0 37.0 37.0 37.0 30-34 35.8516 37.0 37.0 37.0 37.0 37.0 35-39 35.801300000000005 37.0 37.0 37.0 37.0 37.0 40-44 35.8874 37.0 37.0 37.0 37.0 37.0 45-49 35.7642 37.0 37.0 37.0 37.0 37.0 50-54 35.6009 37.0 37.0 37.0 37.0 37.0 55-59 35.664 37.0 37.0 37.0 37.0 37.0 60-64 35.7313 37.0 37.0 37.0 37.0 37.0 65-69 35.6438 37.0 37.0 37.0 37.0 37.0 70-74 35.55030000000001 37.0 37.0 37.0 37.0 37.0 75-79 35.507799999999996 37.0 37.0 37.0 37.0 37.0 80-84 35.5311 37.0 37.0 37.0 37.0 37.0 85-89 35.4118 37.0 37.0 37.0 37.0 37.0 90-94 35.442800000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.4903 37.0 37.0 37.0 37.0 37.0 100-104 35.4459 37.0 37.0 37.0 34.6 37.0 105-109 35.356899999999996 37.0 37.0 37.0 32.2 37.0 110-114 35.2137 37.0 37.0 37.0 29.8 37.0 115-119 35.2661 37.0 37.0 37.0 34.6 37.0 120-124 35.15849999999999 37.0 37.0 37.0 29.8 37.0 125-129 35.0848 37.0 37.0 37.0 27.4 37.0 130-134 35.0625 37.0 37.0 37.0 25.0 37.0 135-139 34.88615 37.0 37.0 37.0 25.0 37.0 140-144 34.761399999999995 37.0 37.0 37.0 25.0 37.0 145-149 34.75245 37.0 37.0 37.0 25.0 37.0 150-151 34.270624999999995 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 3.0 15 4.0 16 1.0 17 4.0 18 7.0 19 2.0 20 7.0 21 5.0 22 9.0 23 15.0 24 16.0 25 10.0 26 17.0 27 20.0 28 17.0 29 14.0 30 28.0 31 46.0 32 52.0 33 120.0 34 271.0 35 775.0 36 2405.0 37 151.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.15 21.05 12.325 28.475 2 26.950000000000003 24.975 29.45 18.625 3 21.099999999999998 27.625 29.75 21.525 4 26.900000000000002 32.574999999999996 23.05 17.474999999999998 5 26.775 34.9 21.925 16.400000000000002 6 21.425 38.15 21.675 18.75 7 21.825 22.95 37.85 17.375 8 22.85 26.25 27.450000000000003 23.45 9 23.474999999999998 23.875 30.3 22.35 10-14 24.560000000000002 29.794999999999998 24.995 20.65 15-19 25.245 27.33 26.265 21.16 20-24 24.845 28.02 26.169999999999998 20.965 25-29 24.83 27.85 26.715 20.605 30-34 24.215 28.765 26.31 20.71 35-39 24.82 28.255000000000003 26.435 20.49 40-44 25.080000000000002 27.865000000000002 26.340000000000003 20.715 45-49 24.905 27.584999999999997 26.474999999999998 21.035 50-54 23.025000000000002 28.515 27.595 20.865000000000002 55-59 24.58 26.85 27.565 21.005 60-64 23.990000000000002 28.165000000000003 26.834999999999997 21.01 65-69 24.89 27.97 26.43 20.71 70-74 25.395 28.845 25.205 20.555 75-79 24.285 27.99 26.5 21.224999999999998 80-84 24.335 28.134999999999998 26.32 21.21 85-89 24.735 28.325 25.72 21.22 90-94 23.76 28.175 27.779999999999998 20.285 95-99 24.9 27.939999999999998 25.86 21.3 100-104 25.1 27.139999999999997 27.04 20.72 105-109 25.224999999999998 28.68 25.835 20.26 110-114 25.36 29.265 25.005 20.369999999999997 115-119 26.314999999999998 28.060000000000002 25.535000000000004 20.09 120-124 25.765 28.375 25.915 19.945 125-129 26.83 27.37 25.669999999999998 20.13 130-134 26.36 28.025 26.064999999999998 19.55 135-139 27.1963598179909 27.971398569928496 25.586279313965697 19.245962298114904 140-144 27.532753275327533 27.277727772777276 26.347634763476346 18.84188418841884 145-149 27.786946736684172 27.321830457614404 26.116529132283073 18.774693673418355 150-151 27.603450431303912 27.203400425053132 25.9407425928241 19.25240655081885 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 1.0 11 1.0 12 0.5 13 0.0 14 0.5 15 1.5 16 1.5 17 1.5 18 2.5 19 3.0 20 2.5 21 2.5 22 2.0 23 1.0 24 2.0 25 2.0 26 1.0 27 2.5 28 5.0 29 4.0 30 5.0 31 7.5 32 13.5 33 22.5 34 28.5 35 51.0 36 73.5 37 84.5 38 107.0 39 143.0 40 181.0 41 202.5 42 232.0 43 264.5 44 274.5 45 312.5 46 302.0 47 254.0 48 230.0 49 186.5 50 159.5 51 141.0 52 131.0 53 99.5 54 69.5 55 67.0 56 57.5 57 43.5 58 33.0 59 40.0 60 33.5 61 18.0 62 10.5 63 9.5 64 8.5 65 2.5 66 1.5 67 4.5 68 8.5 69 5.5 70 0.5 71 1.0 72 3.0 73 3.0 74 1.0 75 0.5 76 0.5 77 0.0 78 0.5 79 1.0 80 0.5 81 3.0 82 4.5 83 2.0 84 1.5 85 1.5 86 0.5 87 1.5 88 2.0 89 0.5 90 0.0 91 1.0 92 1.5 93 0.5 94 0.0 95 0.5 96 1.0 97 1.0 98 1.0 99 1.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.005 140-144 0.01 145-149 0.025 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.3 #Duplication Level Percentage of deduplicated Percentage of total 1 68.64471669218989 44.824999999999996 2 18.83614088820827 24.6 3 7.503828483920367 14.7 4 2.833078101071975 7.3999999999999995 5 0.9954058192955589 3.25 6 0.7274119448698315 2.85 7 0.3062787136294028 1.4000000000000001 8 0.0 0.0 9 0.11485451761102604 0.675 >10 0.03828483920367535 0.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 12 0.3 No Hit CGAGGGTTTGAGCCTTGAGCTCTGTGCAAAGGATAGAGTAGGCTTGCTTT 9 0.22499999999999998 No Hit TGAGACACGAGTTGGCTAGAGATCTTACAAGAGAAACTGCTTGGGAAGCT 9 0.22499999999999998 No Hit GTTTTTGACAATTTCAGTGCAAATGTGGTCGTCAATGGTGCTACTGTTAA 9 0.22499999999999998 No Hit CTCCTCTTCTCTCTTCCTCTTCATTTCCCAATACCTCTGCATCTTCCCTT 7 0.17500000000000002 No Hit GTGTTTCTTCAGTAAGCTTGCCATAATTGTTGGCGGCCCTTTGGTTGGCA 7 0.17500000000000002 No Hit GGAGCAGAAGAACAACAGGCGTGCCCTGTGGATGTCGATGCTCACTCTGC 7 0.17500000000000002 No Hit GCGATCAAACCACCTTTGACGAGTTCTATTCAGAAATGCCTTGGTTAGCC 7 0.17500000000000002 No Hit CTTCTGCAGTGCATGTCTCCTTATATGGATGAAGACATGCTAAATGACCT 7 0.17500000000000002 No Hit GCCACAGGCGTCATACTTCCCAAGAAGCGGCCATAGCCCAGATGCGAGGT 7 0.17500000000000002 No Hit AGCTTATAAAGGGGTTGACAGTGGAGATGGAGGTCCTTTCGGTGCTGTTG 7 0.17500000000000002 No Hit AGCAAGTGCTTCTGTAGCTTTGACATCTTTGTCAAATGAAAAGCTCTTCG 7 0.17500000000000002 No Hit ACCATGTGTAAGAGGCCACAACTGTGCTAGTGTGTGCAAGACTGAAGGTT 6 0.15 No Hit CAACGACTGTTACTCCTCCAAATAGGTCACGCATTCCCCAAAACCCAAAC 6 0.15 No Hit CCTTGATGGCCCAATTCCTGTCCACAAATGTGACTGCAAGTGTCAGAATG 6 0.15 No Hit CGAAGGATCATCAAAGGCAACCAAAAACTGCGTCGGATAAAGAAAGATCT 6 0.15 No Hit TCAGAATTCACCAAATCAGCTGCATCAGTTACTGGGACAGCAGCTGTTTC 6 0.15 No Hit GGCAACTGTTACGTGGAGAGAGAGAAGAGATTTATGTTCAGATTGAGCAA 6 0.15 No Hit GATTTACATGGGGTGAATATATCACTTTTGAAACAGTAGCAGCCAGACTT 6 0.15 No Hit GATGAGTTTAGGTTCCTTAATTACCATTCTGACATTACCAAGGGAGAAGA 6 0.15 No Hit GGGTTCCTCAGCATTGCTGAATTCTTGGGTTGCGGGCTTGATGAATGGCC 6 0.15 No Hit CCGCCCCCCTTCCCACGGCTTGAAATTATTTTTCTCCCATAGCCCCTGCC 6 0.15 No Hit GTCGAAACCAAATATGAAACCCAGTGTCAAGATCATTCATGCCAGGTTGC 6 0.15 No Hit ACTTTCTGTAGGAAAATGGTTTGGTGAAGTTTGCATGTGGAAGAATCCCA 6 0.15 No Hit CGCAATTATCCCCGTCCTGATTTACTGGACTCGCAACGTGGGTCCATCAG 6 0.15 No Hit CACTCTTTCAGACAACAGTCACAAAACCAGTGAAGAAAAAAAAATGTCGT 6 0.15 No Hit GCCCCGCGCGTACTAGGCAGATAAGAACCCTCCAGCTCGGGGCCTCAAAC 6 0.15 No Hit GTGAGGTATTTTTTCAATGTTAGATCATCTATGTAGTGGATCTGAGAGAT 6 0.15 No Hit GCCATTGGAAATGCTGGTTCTGCAATAGGAATATTATCCAAAGATGGGGT 6 0.15 No Hit GAAAGATTGTGCATACAGCCCAGTTGACTAGCAATGCCTTGGCTTTGATC 6 0.15 No Hit GAAAAGCCATTCTGGATCATCAAGAACTCCTGGGGAGAAAAGTGGGGAGA 6 0.15 No Hit GAAGTTCAGATCAAGAAATAAAAAGGTTTGTTTTGGGCCAATACAACCGC 5 0.125 No Hit GAGATACTTGCTGCTGTTGCTTGCGACAACAGTATGATTAATGATGCTGC 5 0.125 No Hit CCGAGAGCTCAGTGCCGCTTGCCTCTCTATCTCTTCGATTTCCAGGTAAC 5 0.125 No Hit CTTGGAACGCACCAAAGAGAGGGGACTAGTGGTGAAGTTATGGGCACCTC 5 0.125 No Hit GCTAGACATAATTCTTACTAAGGGAGGTTATGGTGGGGACAAACCCGGTT 5 0.125 No Hit CCGAGGGAGAAAATTTAGCCCTCTCTCTCCTCTTTGTTTTGCCTCCCATC 5 0.125 No Hit ACGATCGCGATCAAAGGAGCGGAAGAATGAGATTAATCTTAGTGCTTGCT 5 0.125 No Hit TCTTGCAATGAGTTTCTACTTCAATTCTAGCTCGGCTGCTAGAGTCACCC 5 0.125 No Hit TTCAGCTCAAAGGGCATAGGTCAGTGGGAGGAATGAGAGCTAGCATTTAT 5 0.125 No Hit TATTGGAGGTATTGGTATTGGTGGAGGTATTCATAATATTGGAGATATGG 5 0.125 No Hit AGGCTATTTCCCTGTGAAGTGGCACATTGTGAAGGATGTCCCCAACAGTT 5 0.125 No Hit CGAGATCAACCAGCTCTTGAGCTTGATCATCAATACTTTCTACAGCAACA 5 0.125 No Hit CAGATATTAAGCATGAGTAGACTGGGCGGAGCTGGTGCTGTTGCGCCACT 5 0.125 No Hit CTCGAGAATCATCAGATGTAAGAAGGCTTGCCAATTTCTACAAAGACATA 5 0.125 No Hit CAATCAACAAGATCACAGGGGCGGCGCGCAAGGTGTGCGCAAGGAAGGGT 5 0.125 No Hit GAAAAAATGAGGAGAGCCCATTGCACTTTTCTGCTTTGCTTTGCCTTCGT 5 0.125 No Hit AAAGAATCCGGTATTGCAGGAAGTGCAACCAGCTGAAACCACCTCGTTGC 5 0.125 No Hit GTCAAGACAGGGAGTCATTCAGTAAATATGGCAACAACTTTGCAAATCCT 5 0.125 No Hit GACTAAAGAGCAGAGGAGTCGCAATAGCCGATAGAGAGGGAGAGCGAGCT 5 0.125 No Hit GACAACGTTTTGCTGGAGTGGACATGAGGATCCGTGTCAAGGGTGGAGGA 5 0.125 No Hit GGCTGATGATCTCACAAGTTCTCAGCCTTCTTTTTTGTCTGAAACAAGCG 5 0.125 No Hit GGCATGAAAAAGCTAGCACCATGGGTTTCGTGATAATTCTCTCCATAGAG 5 0.125 No Hit GGATTGTTAACCTATCTTCATCAGCTCATCGTCATCCATTTCCTGGAGGA 5 0.125 No Hit GGTCAAGATCATGCTTGACTGGGATCCTAAGGGCAAGGTGGGGCCCATGA 5 0.125 No Hit ACTGAAGATGCTGAGAAGAACTTTGCAGAGCTACTGAAAGAGGAATTGCA 5 0.125 No Hit CCATCCTAATCAGTTTGGCTTTCCAACACCACCACTTTTTCCACCAAACC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.05 0.0 0.0 0.0 0.0 32-33 0.05 0.0 0.0 0.0 0.0 34-35 0.05 0.0 0.0 0.0 0.0 36-37 0.05 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.2 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.30000000000000004 0.0 0.0 0.0 0.0 82-83 0.375 0.0 0.0 0.0 0.0 84-85 0.4875 0.0 0.0 0.0 0.0 86-87 0.6625000000000001 0.0 0.0 0.0 0.0 88-89 0.9750000000000001 0.0 0.0 0.0 0.0 90-91 1.0875 0.0 0.0 0.0 0.0 92-93 1.2375 0.0 0.0 0.0 0.0 94-95 1.4375 0.0 0.0 0.0 0.0 96-97 1.6375 0.0 0.0 0.0 0.0 98-99 1.9625 0.0 0.0 0.0 0.0 100-101 2.2249999999999996 0.0 0.0 0.0 0.0 102-103 2.5875 0.0 0.0 0.0 0.0 104-105 2.9125 0.0 0.0 0.0 0.0 106-107 3.6125 0.0 0.0 0.0 0.0 108-109 4.125 0.0 0.0 0.0 0.0 110-111 4.45 0.0 0.0 0.0 0.0 112-113 4.925 0.0 0.0 0.0 0.0 114-115 5.7375 0.0 0.0 0.0 0.0 116-117 6.4 0.0 0.0 0.0 0.0 118-119 6.862500000000001 0.0 0.0 0.0 0.0 120-121 7.362500000000001 0.0 0.0 0.0 0.0 122-123 8.075 0.0 0.0 0.0 0.0 124-125 8.8375 0.0 0.0 0.0 0.0 126-127 9.524999999999999 0.0 0.0 0.0 0.0 128-129 10.2 0.0 0.0 0.0 0.0 130-131 10.837499999999999 0.0 0.0 0.0 0.0 132-133 11.45 0.0 0.0 0.0 0.0 134-135 12.2 0.0 0.0 0.0 0.0 136-137 12.9125 0.0 0.0 0.0 0.0 138-139 13.6375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCCTTG 10 0.006830828 145.0 5 >>END_MODULE Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra Read 2793833 spots for SRR26075341.sra Written 2793833 spots for SRR26075341.sra Read 2793829 spots for SRR26075341.sra Written 2793829 spots for SRR26075341.sra SRR ids: ['SRR26075341.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_1m5yofec SRR26075341.sra spots: 55876584 blocks: [[1, 2793829], [2793830, 5587658], [5587659, 8381487], [8381488, 11175316], [11175317, 13969145], [13969146, 16762974], [16762975, 19556803], [19556804, 22350632], [22350633, 25144461], [25144462, 27938290], [27938291, 30732119], [30732120, 33525948], [33525949, 36319777], [36319778, 39113606], [39113607, 41907435], [41907436, 44701264], [44701265, 47495093], [47495094, 50288922], [50288923, 53082751], [53082752, 55876584]] SRR26075341 file size 20640856 SRR26075341 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075341 SRR26075341_1.fastq SRR26075341_2.fastq Input file: SRR26075341_1.fastq Paired file: SRR26075341_2.fastq trimmed: SRR26075341-trimmed-pair1.fastq, SRR26075341-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 21:45:42 2025 >> started Tue Feb 11 21:46:47 2025 >> done (65.017s) 55876584 read pairs processed; of these: 411 ( 0.00%) short read pairs filtered out after trimming by size control 297088 ( 0.53%) empty read pairs filtered out after trimming by size control 55579085 (99.47%) read pairs available; of these: 10205746 (18.36%) trimmed read pairs available after processing 45373339 (81.64%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 29 0.00% 19 27 0.00% 20 38 0.00% 21 40 0.00% 22 46 0.00% 23 45 0.00% 24 50 0.00% 25 54 0.00% 26 68 0.00% 27 77 0.00% 28 108 0.00% 29 84 0.00% 30 78 0.00% 31 79 0.00% 32 106 0.00% 33 106 0.00% 34 124 0.00% 35 104 0.00% 36 141 0.00% 37 125 0.00% 38 134 0.00% 39 143 0.00% 40 157 0.00% 41 166 0.00% 42 204 0.00% 43 242 0.00% 44 251 0.00% 45 253 0.00% 46 280 0.00% 47 340 0.00% 48 325 0.00% 49 387 0.00% 50 403 0.00% 51 487 0.00% 52 594 0.00% 53 708 0.00% 54 681 0.00% 55 858 0.00% 56 894 0.00% 57 1025 0.00% 58 1192 0.00% 59 1431 0.00% 60 1643 0.00% 61 1968 0.00% 62 2285 0.00% 63 2573 0.00% 64 3057 0.01% 65 3283 0.01% 66 3531 0.01% 67 4335 0.01% 68 4665 0.01% 69 5493 0.01% 70 6244 0.01% 71 7203 0.01% 72 8288 0.01% 73 9506 0.02% 74 11003 0.02% 75 12052 0.02% 76 13888 0.02% 77 15476 0.03% 78 16810 0.03% 79 18611 0.03% 80 20740 0.04% 81 22751 0.04% 82 25466 0.05% 83 29024 0.05% 84 32038 0.06% 85 35344 0.06% 86 37453 0.07% 87 40056 0.07% 88 43171 0.08% 89 45485 0.08% 90 47990 0.09% 91 51796 0.09% 92 56246 0.10% 93 60876 0.11% 94 65845 0.12% 95 69840 0.13% 96 73396 0.13% 97 77136 0.14% 98 79088 0.14% 99 83953 0.15% 100 85472 0.15% 101 88826 0.16% 102 93139 0.17% 103 98432 0.18% 104 103212 0.19% 105 106852 0.19% 106 112079 0.20% 107 116396 0.21% 108 118843 0.21% 109 122733 0.22% 110 123710 0.22% 111 128384 0.23% 112 130503 0.23% 113 134152 0.24% 114 140337 0.25% 115 146310 0.26% 116 149808 0.27% 117 152464 0.27% 118 159251 0.29% 119 159738 0.29% 120 160904 0.29% 121 163489 0.29% 122 167738 0.30% 123 171157 0.31% 124 176367 0.32% 125 181297 0.33% 126 185171 0.33% 127 188673 0.34% 128 193979 0.35% 129 195627 0.35% 130 198627 0.36% 131 202033 0.36% 132 202109 0.36% 133 207304 0.37% 134 208558 0.38% 135 212678 0.38% 136 216459 0.39% 137 219956 0.40% 138 223479 0.40% 139 228214 0.41% 140 231776 0.42% 141 231610 0.42% 142 236751 0.43% 143 236138 0.42% 144 239395 0.43% 145 243727 0.44% 146 244223 0.44% 147 247290 0.44% 148 251445 0.45% 149 251819 0.45% 150 254820 0.46% 151 45373339 81.64% 55579085 reads passed initial QC criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=2.31 fanout-score-rank=34 prefix-density=0.35 prefix-fanout=2.3 sequence=GTCAGGGTACAT criterion=fanout-score sequence-density=0.15 sequence-density-rank=23 fanout-score=23.89 fanout-score-rank=1 prefix-density=0.42 prefix-fanout=8.4 sequence=TCTTCTCATCAA criterion=sequence-density sequence-density=0.58 sequence-density-rank=1 fanout-score=2.27 fanout-score-rank=29 prefix-density=0.60 prefix-fanout=2.2 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=187.52 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=15.0 sequence=AGAGAAAAGATAAGCTAGGCAAGATGGTTTTACTA SRR26075341 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 21:47:29 Started mapping on | Feb 11 21:47:29 Finished on | Feb 11 21:59:45 Mapping speed, Million of reads per hour | 271.85 Number of input reads | 55579085 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 47621429 Uniquely mapped reads % | 85.68% Average mapped length | 291.00 Number of splices: Total | 46260661 Number of splices: Annotated (sjdb) | 45117265 Number of splices: GT/AG | 45383861 Number of splices: GC/AG | 666450 Number of splices: AT/AC | 52792 Number of splices: Non-canonical | 157558 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 3.13 Insertion rate per base | 0.02% Insertion average length | 2.56 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1268836 % of reads mapped to multiple loci | 2.28% Number of reads mapped to too many loci | 989273 % of reads mapped to too many loci | 1.78% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 9.75% % of reads unmapped: other | 0.50% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6688820 6688820 6688820 N_multimapping 1268836 1268836 1268836 N_noFeature 1269275 47189118 1516412 N_ambiguous 427446 2850 240562 UnstrandedReadsAssigned:45924708 PositiveStrandReadsAssigned:429461 NegativeStrandReadsAssigned:45864455 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075341 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075341-trimmed-pair1.fastq SRR26075341-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 55,579,085 reads, 46,880,535 reads pseudoaligned [quant] estimated average fragment length: 209.321 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,259 rounds 52401 SRR26075341.ke.tsv 34699 SRR26075341.se.tsv 87100 total ==> SRR26075341.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1809.68 5107 55.0923 Potri.005G024800.1.v4.1 1035 826.679 6290 148.539 Potri.004G059700.1.v4.1 961 752.679 0 0 Potri.007G009000.2.v4.1 1416 1207.68 0 0 Potri.003G141000.2.v4.1 2943 2734.68 2389.28 17.0564 Potri.016G087400.1.v4.1 270 94.7123 3933.53 810.779 Potri.015G069301.1.v4.1 564 358.007 0 0 Potri.010G195200.1.v4.1 1773 1564.68 3475 43.3567 Potri.012G127500.1.v4.1 977 768.679 26745 679.242 ==> SRR26075341.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 50 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 677 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 3253 SRR26075341 completed mapping pipeline successfully