Starting /dee2/code/volunteer_pipeline.sh SRR26075342 current disk space = 3053024415744 free memory = 1429418008 SRR26075342 SRAfilesize 949d25c515caf0e500b554f906045fa0 SRR26075342.sra SRR26075342.sra file validated SRR26075342 is paired end SRR26075342 is conventional basespace SRR26075342 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075342_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.47475 37.0 37.0 37.0 37.0 37.0 2 36.567 37.0 37.0 37.0 37.0 37.0 3 36.6675 37.0 37.0 37.0 37.0 37.0 4 36.701 37.0 37.0 37.0 37.0 37.0 5 36.7175 37.0 37.0 37.0 37.0 37.0 6 36.7045 37.0 37.0 37.0 37.0 37.0 7 36.6205 37.0 37.0 37.0 37.0 37.0 8 36.672 37.0 37.0 37.0 37.0 37.0 9 36.677 37.0 37.0 37.0 37.0 37.0 10-14 36.6513 37.0 37.0 37.0 37.0 37.0 15-19 36.60979999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.55749999999999 37.0 37.0 37.0 37.0 37.0 25-29 36.4902 37.0 37.0 37.0 37.0 37.0 30-34 36.4445 37.0 37.0 37.0 37.0 37.0 35-39 36.3928 37.0 37.0 37.0 37.0 37.0 40-44 36.3423 37.0 37.0 37.0 37.0 37.0 45-49 36.265299999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.287699999999994 37.0 37.0 37.0 37.0 37.0 55-59 36.2195 37.0 37.0 37.0 37.0 37.0 60-64 36.180800000000005 37.0 37.0 37.0 37.0 37.0 65-69 36.12220000000001 37.0 37.0 37.0 37.0 37.0 70-74 36.1202 37.0 37.0 37.0 37.0 37.0 75-79 36.0164 37.0 37.0 37.0 37.0 37.0 80-84 35.9927 37.0 37.0 37.0 37.0 37.0 85-89 35.961099999999995 37.0 37.0 37.0 37.0 37.0 90-94 35.920899999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.946999999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.9231 37.0 37.0 37.0 37.0 37.0 105-109 35.795500000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.6424 37.0 37.0 37.0 37.0 37.0 115-119 35.5775 37.0 37.0 37.0 37.0 37.0 120-124 35.5644 37.0 37.0 37.0 37.0 37.0 125-129 35.380900000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.3082 37.0 37.0 37.0 34.6 37.0 135-139 35.155199999999994 37.0 37.0 37.0 27.4 37.0 140-144 34.9877 37.0 37.0 37.0 25.0 37.0 145-149 34.872699999999995 37.0 37.0 37.0 25.0 37.0 150-151 34.781 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 1.0 20 2.0 21 2.0 22 3.0 23 3.0 24 4.0 25 3.0 26 4.0 27 12.0 28 16.0 29 20.0 30 28.0 31 48.0 32 87.0 33 78.0 34 180.0 35 494.0 36 2832.0 37 182.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.57619884509164 13.93422043685664 8.787346221441124 41.70223449661059 2 19.475 14.224999999999998 36.625 29.675 3 17.175 17.424999999999997 28.999999999999996 36.4 4 21.8 23.375 24.675 30.15 5 22.6 30.0 26.150000000000002 21.25 6 24.425 31.225 22.0 22.35 7 14.524999999999999 28.675 40.725 16.075 8 18.2 25.874999999999996 32.074999999999996 23.849999999999998 9 19.55 23.25 34.175 23.025000000000002 10-14 19.275000000000002 29.01 28.125 23.59 15-19 20.200000000000003 27.685 27.975 24.14 20-24 19.975 28.005000000000003 27.435 24.585 25-29 19.175 27.785 27.82 25.22 30-34 19.68 26.945000000000004 28.49 24.884999999999998 35-39 19.814999999999998 27.905 26.985 25.295 40-44 19.744999999999997 27.875 28.68 23.7 45-49 21.085 26.68 27.79 24.445 50-54 19.605 27.36 28.375 24.66 55-59 19.46 27.279999999999998 28.050000000000004 25.21 60-64 21.505 26.889999999999997 27.615000000000002 23.990000000000002 65-69 20.380000000000003 27.555000000000003 27.37 24.695 70-74 21.195 27.560000000000002 28.215 23.03 75-79 20.57 27.275 27.515 24.64 80-84 21.005 26.840000000000003 27.91 24.245 85-89 20.73 27.105 26.875 25.290000000000003 90-94 20.845 26.345000000000002 28.68 24.13 95-99 21.555 26.63 27.944999999999997 23.87 100-104 20.71 27.505000000000003 27.339999999999996 24.445 105-109 21.005 27.500000000000004 27.625 23.87 110-114 21.0 27.825 26.900000000000002 24.275 115-119 20.625 27.935 26.61 24.83 120-124 21.740000000000002 27.92 26.035000000000004 24.305 125-129 21.985 27.1 26.51 24.404999999999998 130-134 20.995 26.87 27.584999999999997 24.55 135-139 21.705 27.105 25.990000000000002 25.2 140-144 21.665 27.435 26.314999999999998 24.585 145-149 22.79 26.340000000000003 26.645000000000003 24.224999999999998 150-151 22.9375 27.725 26.5375 22.8 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 2.0 24 1.5 25 2.5 26 10.5 27 12.0 28 9.5 29 12.5 30 12.5 31 12.0 32 21.0 33 22.0 34 31.0 35 52.0 36 70.0 37 89.5 38 113.0 39 149.5 40 168.0 41 183.5 42 228.0 43 276.5 44 281.5 45 272.5 46 276.5 47 273.0 48 262.5 49 225.5 50 163.0 51 131.0 52 117.5 53 101.5 54 81.5 55 61.0 56 52.5 57 44.5 58 34.0 59 29.5 60 24.5 61 14.5 62 14.5 63 14.5 64 10.5 65 5.0 66 3.0 67 1.5 68 3.5 69 4.5 70 4.0 71 3.5 72 2.5 73 2.0 74 1.5 75 1.0 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42500000000000004 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.125 #Duplication Level Percentage of deduplicated Percentage of total 1 65.07042253521126 40.425 2 19.839034205231385 24.65 3 9.456740442655935 17.625 4 3.0181086519114686 7.5 5 1.488933601609658 4.625 6 0.5633802816901409 2.1 7 0.12072434607645875 0.525 8 0.16096579476861167 0.8 9 0.16096579476861167 0.8999999999999999 >10 0.12072434607645875 0.8500000000000001 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTCACGCCTTCATTGTCCAATAATCCCGGTCTTCCACCTTTTTTTTTAT 13 0.325 No Hit GAGAGAACATGTTAATTTTCTCATACTCTACAGTCCCCAACAAGTTCTCT 11 0.27499999999999997 No Hit TGTAAACACTCTGTGGAATCGGGCTCAGGACATAGAGAGTTGTTCCGCTG 10 0.25 No Hit CCTTATTGGAGCTGAAGTGGTCACAGCATCTCTCAGATCTCTCCCAGCTT 9 0.22499999999999998 No Hit CTGATTTGGTGGCATAGCATATCCATATCCAGCATATCCTTGCCCTCCAA 9 0.22499999999999998 No Hit GAGTTGATTGTTAACACTGCACAGTACATTCCTCCATAATCTTGGCCCTT 9 0.22499999999999998 No Hit CTCTAATATCATCAGTTACTGTCCCAACAAGCACAGCAATCTTGTCCTCC 9 0.22499999999999998 No Hit GCAGGGAGGTGGAGAAGGGAATGGCGGCCAGGTGCTGGGGAAAAGAAAAA 8 0.2 No Hit CTTGCATTTCCATATCTTCTTCCTCTTTTAATGTCTTCTTCTCTGTTAAC 8 0.2 No Hit TCTGGCTCGGGAGGTGGAGGAGTGTAATTCTCCGGTGGAGGCAGGGCCTT 8 0.2 No Hit CCACCCTGATCTCTAAACATGCGCATTGCCACTTCAGGTGGGGCAGGAAC 8 0.2 No Hit GTAGCATGAACAAGGACCCAAAACCCAGAAAGAAAGAGGCCAATGCTCCT 7 0.17500000000000002 No Hit GCCTGAAGAAGGGTGGAGAGCTCATCCAAGTCTTCAATGGATGTATGAGG 7 0.17500000000000002 No Hit GTCCTTGCTAGAGCTAAACAATTTTGGCTTTTTGGCAGGAGATTCTGGTT 7 0.17500000000000002 No Hit CTTCTCTGCAGTCATGTTGGCATAACTTTGATTACGATCAAGATGAAAAG 6 0.15 No Hit CCTATGTGAAACAAACTCAAATGTAATCTTCAAATTTCAACCTGTTTTAG 6 0.15 No Hit TCTCTATTGCCAAAACCGCTGCTCATTCCATCTCTATTGCCAAAACCTCT 6 0.15 No Hit CTTCATTCTTCTTCTTGTCCTTTTTCTTCTTTTTCTCTCCTCCTTCACCA 6 0.15 No Hit CCTATGATGTGCATTATGTGGAAAATCCTCCTGCACATTAGTTTCCCAAA 6 0.15 No Hit GCATCCTTGTCAGCACCAGAGGCTAGAGCATTTTTCAAACCCTCAACATC 6 0.15 No Hit AGCCAACGTCGGTCAAGGGGGTGAGTCCGTTTCCAAAAAGTAATGAGGCC 6 0.15 No Hit GACGGTTCTGGAAACCAACTGCACCCACAACTTGCATAGGGTTCATCCCA 6 0.15 No Hit AGGGCATGGTGTTTGTTGGAGGAGAGGCAGATTGGGGCACGAGGTGATTT 6 0.15 No Hit GGGTAATATTCACTGGGATGAAACATACAGCATTCACCATCCTCAGTCAT 6 0.15 No Hit CAGATCTTGGTCTTGAAGTTGTTTGATCCACCAGAAGAGTGCATTGCTTG 6 0.15 No Hit TTCCCATGAAAAATCAGCTGACTTGATTAAAACAGAATGGTTCACACTCC 6 0.15 No Hit GGCAGACACATCCCACAAACATATTCTATTATCATGTGAGCCACTAACAA 6 0.15 No Hit GCTGGGTGGACTGGTCGACCCCGACGCCGTACGAACGGAGGCCACGGAAG 6 0.15 No Hit GGCTGAACCTGAAATTTCAGGCGGAGCAGCAACAGCCTTGTCAGAGCTTT 5 0.125 No Hit GGCTTATATCGCATCTCATCTGTAATAGGAGGAATAAGGGATTCTGGAAC 5 0.125 No Hit GCTGGATGGACAGGCTCTTCTATCTTTACAAACTGGAACAACTGGCAGAG 5 0.125 No Hit CCTCACGGTAGTTCCCTGAAGACAGGGAGGGAGTCCTGGCTGGTAAAGAG 5 0.125 No Hit CCACATACAGCCTCACCAATCAAGTTCTTCATATCAATGCGACATTCGAC 5 0.125 No Hit GGAACGGTCTCATCATCAGCATCATCATCATCTTGCACCATGGGCGTATG 5 0.125 No Hit CACTTATCATCAACAGGCCGACTTTGCTCTCTTTCAGCAGCAAACTCATT 5 0.125 No Hit CACCGACTGCTTCATATTGTTTCCACGCTTGATAATAACCACCAATGTCC 5 0.125 No Hit GGATCTTGATGCTGCAGGTCCAAGTTTATCAGTTCCTGCAGCTTTCTTTT 5 0.125 No Hit GGTCTCAACAACCATAGGCTTGGTGGGAATCATCTTCACAAACCCAGCAT 5 0.125 No Hit CCAGTGCATTGACCCTACGATTTGTGGTCTTGATAGCCGTATCAAGCGTC 5 0.125 No Hit ATCTTTATTATCTTCCCCGATCTCCACACTTGGCATGGCCTCTATGGATG 5 0.125 No Hit GTCCTGACTAAAAGTCATATCCCTGCGCTCAAACATTGCCGGAAGTGGTC 5 0.125 No Hit GGGGGTAGCGAGCTTGAGAGTGCGGAAGCAGATGTCATACAAAGCTTCAT 5 0.125 No Hit CTTAACTTTGGGAGTTTGAATTCGGTGTTGTTGCTGCTGCTTTCTTCATC 5 0.125 No Hit GACAATGACTAAGCACAAGCAAACTGTTAAATTCTGGAGGAGGGTGTGAG 5 0.125 No Hit AGTGTCTGTCTGCCCACCACATCCGGAGGGAGGACCATTACAGTTTAAGC 5 0.125 No Hit CCATCTACATCACACGTCATGGAAGCATCTTCATATACGCCTCTTCTCAC 5 0.125 No Hit GGTAAGGTGTGTTTCTACTGCTATGAGCAAGGTAAAGGTAAAGCAATCAT 5 0.125 No Hit CCTCAATATTTCAATCTGAGCCATCATTAGTGCCACATAGACACCAGCAG 5 0.125 No Hit CTGTAGTATCCAACACTTGATCATAGCATGAAAAGGTGTTGCTAGGAATA 5 0.125 No Hit ACCGCCTGGAGGCATTAGATTTCCTGACAGTCACATGTAACTTGCCATCA 5 0.125 No Hit CTAGCAAGTCTTGAGGATTCCTGAGCAAGCTTCTCGAAAATATCATTAAT 5 0.125 No Hit CATCAAGCATGGCAGTGCCAATCTTCGCTGGAGATTCAACCACTATCACC 5 0.125 No Hit GTTTCGCTGGATAGTAGACAGGGACAGTGGGAATCTCGTTAATCCATTCA 5 0.125 No Hit CCCTAAGTCGAGTTTTAAAACTACGATAATAATCATTTTTATCCTTACAT 5 0.125 No Hit CTCGCAGCTCTTGTCCTTAAAAGGCAATGCCAACCAAGGCATTGTCTCAA 5 0.125 No Hit CTCAGAAATAGCCTCGTCAAAAGCCTGCTTGGCAAGATTGCAAGCACGAT 5 0.125 No Hit GTTATCATAAAGGGGAGCATCTGACCCCTTTAACTTCTTGCGAGGTGGGC 5 0.125 No Hit CTCTAACAAAACACGCACTGAATCACGGCATTTCATGAACAGGTATTTTC 5 0.125 No Hit CCGGAAGCAACTTTAGAACTTTTAGTGCTGCCACGCTAATGTTCGCATTG 5 0.125 No Hit CTCATGGTCCATAATGCCCGCAGAAGTTGTCAAGACAATATATCCAAACT 5 0.125 No Hit CGAGAAACGGCACGACCAGTACCCCATGATTCGGCTGAGGTCTGGTGACC 5 0.125 No Hit ATTATTATCGTCTATTCGTGTACAAAAAATTTACTTCAAAAACAGGAGAA 5 0.125 No Hit CACCTTCTGAGCAATTGTGCCACCATTAACCTGGATCTCCATCAAGTGAG 5 0.125 No Hit GGGTATTGATAGCAAAAAGGTCAGGAAGTTGAGTACCCCTATCAAGTGAT 5 0.125 No Hit CCTGCAAGAACATCTGGAATGAGCCCACCAAATGGACTAACCAGTCTGTA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0125 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.037500000000000006 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.15 0.0 0.0 0.0 0.0 70-71 0.1875 0.0 0.0 0.0 0.0 72-73 0.225 0.0 0.0 0.0 0.0 74-75 0.275 0.0 0.0 0.0 0.0 76-77 0.2875 0.0 0.0 0.0 0.0 78-79 0.3 0.0 0.0 0.0 0.0 80-81 0.3 0.0 0.0 0.0 0.0 82-83 0.3 0.0 0.0 0.0 0.0 84-85 0.4375 0.0 0.0 0.0 0.0 86-87 0.6375 0.0 0.0 0.0 0.0 88-89 0.8999999999999999 0.0 0.0 0.0 0.0 90-91 1.0625 0.0 0.0 0.0 0.0 92-93 1.1875 0.0 0.0 0.0 0.0 94-95 1.4875 0.0 0.0 0.0 0.0 96-97 1.6749999999999998 0.0 0.0 0.0 0.0 98-99 1.875 0.0 0.0 0.0 0.0 100-101 2.2375 0.0 0.0 0.0 0.0 102-103 2.5 0.0 0.0 0.0 0.0 104-105 2.8375 0.0 0.0 0.0 0.0 106-107 3.4625000000000004 0.0 0.0 0.0 0.0 108-109 3.825 0.0 0.0 0.0 0.0 110-111 4.125 0.0 0.0 0.0 0.0 112-113 4.7 0.0 0.0 0.0 0.0 114-115 5.0875 0.0 0.0 0.0 0.0 116-117 5.5 0.0 0.0 0.0 0.0 118-119 6.0625 0.0 0.0 0.0 0.0 120-121 6.625 0.0 0.0 0.0 0.0 122-123 7.0625 0.0 0.0 0.0 0.0 124-125 7.475 0.0 0.0 0.0 0.0 126-127 8.05 0.0 0.0 0.0 0.0 128-129 8.8 0.0 0.0 0.0 0.0 130-131 9.7 0.0 0.0 0.0 0.0 132-133 10.149999999999999 0.0 0.0 0.0 0.0 134-135 10.8375 0.0 0.0 0.0 0.0 136-137 11.537500000000001 0.0 0.0 0.0 0.0 138-139 12.125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCAAA 10 0.006577216 146.82278 1 TTTTAGA 10 0.006832588 144.9875 5 CAATAAT 10 0.006832588 144.9875 145 AGCAAAT 10 0.006832588 144.9875 2 AGATTGT 10 0.006832588 144.9875 9 >>END_MODULE SRR26075342 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075342_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.20975 37.0 37.0 37.0 37.0 37.0 2 36.156 37.0 37.0 37.0 37.0 37.0 3 36.1325 37.0 37.0 37.0 37.0 37.0 4 36.193 37.0 37.0 37.0 37.0 37.0 5 36.33 37.0 37.0 37.0 37.0 37.0 6 36.0665 37.0 37.0 37.0 37.0 37.0 7 36.0865 37.0 37.0 37.0 37.0 37.0 8 36.191 37.0 37.0 37.0 37.0 37.0 9 36.2105 37.0 37.0 37.0 37.0 37.0 10-14 36.1723 37.0 37.0 37.0 37.0 37.0 15-19 36.0986 37.0 37.0 37.0 37.0 37.0 20-24 36.0931 37.0 37.0 37.0 37.0 37.0 25-29 35.9465 37.0 37.0 37.0 37.0 37.0 30-34 35.8856 37.0 37.0 37.0 37.0 37.0 35-39 35.8343 37.0 37.0 37.0 37.0 37.0 40-44 35.8085 37.0 37.0 37.0 37.0 37.0 45-49 35.6911 37.0 37.0 37.0 37.0 37.0 50-54 35.6027 37.0 37.0 37.0 37.0 37.0 55-59 35.640499999999996 37.0 37.0 37.0 37.0 37.0 60-64 35.7491 37.0 37.0 37.0 37.0 37.0 65-69 35.594 37.0 37.0 37.0 37.0 37.0 70-74 35.55 37.0 37.0 37.0 37.0 37.0 75-79 35.4657 37.0 37.0 37.0 37.0 37.0 80-84 35.5047 37.0 37.0 37.0 37.0 37.0 85-89 35.5279 37.0 37.0 37.0 37.0 37.0 90-94 35.438300000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.3809 37.0 37.0 37.0 37.0 37.0 100-104 35.3384 37.0 37.0 37.0 37.0 37.0 105-109 35.2522 37.0 37.0 37.0 34.6 37.0 110-114 35.234899999999996 37.0 37.0 37.0 32.2 37.0 115-119 35.1714 37.0 37.0 37.0 32.2 37.0 120-124 35.085 37.0 37.0 37.0 27.4 37.0 125-129 35.0102 37.0 37.0 37.0 25.0 37.0 130-134 35.037600000000005 37.0 37.0 37.0 27.4 37.0 135-139 34.85275 37.0 37.0 37.0 25.0 37.0 140-144 34.90155 37.0 37.0 37.0 25.0 37.0 145-149 34.72745 37.0 37.0 37.0 25.0 37.0 150-151 34.511375 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 4.0 14 8.0 15 11.0 16 9.0 17 5.0 18 4.0 19 3.0 20 4.0 21 6.0 22 13.0 23 8.0 24 10.0 25 12.0 26 14.0 27 9.0 28 18.0 29 23.0 30 27.0 31 35.0 32 54.0 33 104.0 34 228.0 35 755.0 36 2460.0 37 176.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.735183795948984 20.7551887971993 11.77794448612153 26.731682920730183 2 28.325 24.9 28.375 18.4 3 20.424999999999997 29.5 30.75 19.325 4 23.0 34.725 23.825 18.45 5 26.025 35.475 22.15 16.35 6 22.575 35.625 22.925 18.875 7 20.25 20.625 39.074999999999996 20.05 8 19.925 25.5 27.224999999999998 27.35 9 23.0 25.95 27.875 23.175 10-14 24.23 29.505 24.645 21.62 15-19 24.22 28.625 26.995 20.16 20-24 24.610000000000003 28.57 26.35 20.47 25-29 23.985 28.549999999999997 26.8 20.665 30-34 24.705 28.904999999999998 25.72 20.669999999999998 35-39 24.855 28.035 26.029999999999998 21.08 40-44 24.585 28.89 25.09 21.435000000000002 45-49 24.665 28.03 26.08 21.224999999999998 50-54 24.01 27.744999999999997 27.32 20.925 55-59 24.39 29.054999999999996 26.700000000000003 19.855 60-64 25.19 28.16 26.314999999999998 20.335 65-69 25.255 28.549999999999997 25.455 20.74 70-74 25.045 29.330000000000002 25.624999999999996 20.0 75-79 24.79 28.265 26.11 20.835 80-84 24.85 28.735 25.674999999999997 20.74 85-89 24.87 28.660000000000004 26.200000000000003 20.27 90-94 25.319999999999997 28.360000000000003 25.845000000000002 20.474999999999998 95-99 24.575 28.810000000000002 25.635 20.979999999999997 100-104 25.290000000000003 29.154999999999998 25.605 19.950000000000003 105-109 25.445 28.73 26.08 19.744999999999997 110-114 25.825 28.02 26.064999999999998 20.09 115-119 25.380000000000003 28.999999999999996 25.900000000000002 19.72 120-124 25.919999999999998 28.51 26.145000000000003 19.425 125-129 26.584999999999997 28.785 25.650000000000002 18.98 130-134 26.525 28.470000000000002 25.825 19.18 135-139 25.871293564678233 27.796389819490976 26.521326066303313 19.810990549527478 140-144 27.24908736310447 28.4742711406711 25.403810571585737 18.872830924638695 145-149 26.676669167291823 28.592148037009252 25.82145536384096 18.909727431857963 150-151 26.003250406300786 28.641080135016878 26.52831603950494 18.8273534191774 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.5 5 0.5 6 0.5 7 1.5 8 1.0 9 0.0 10 1.0 11 1.5 12 1.0 13 1.0 14 0.5 15 0.5 16 1.0 17 0.5 18 0.5 19 1.5 20 2.5 21 2.0 22 1.5 23 2.5 24 4.0 25 3.0 26 0.5 27 2.0 28 5.5 29 8.0 30 9.5 31 8.5 32 10.5 33 15.0 34 19.0 35 52.5 36 68.0 37 71.0 38 96.5 39 147.0 40 215.5 41 234.5 42 242.0 43 259.0 44 280.5 45 296.5 46 271.0 47 261.0 48 241.5 49 211.0 50 189.0 51 146.5 52 106.0 53 94.0 54 79.5 55 56.0 56 51.0 57 36.5 58 31.0 59 21.5 60 11.0 61 14.5 62 14.5 63 11.5 64 8.0 65 5.5 66 6.0 67 4.0 68 2.0 69 3.5 70 3.0 71 2.0 72 2.0 73 2.0 74 2.5 75 1.5 76 1.0 77 1.0 78 0.5 79 1.5 80 1.5 81 2.5 82 3.5 83 2.0 84 2.0 85 2.0 86 2.0 87 1.5 88 0.5 89 1.0 90 0.5 91 1.5 92 2.0 93 1.0 94 1.0 95 0.5 96 0.5 97 0.5 98 0.5 99 1.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.005 140-144 0.015 145-149 0.025 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.324999999999996 #Duplication Level Percentage of deduplicated Percentage of total 1 66.79826292933281 42.3 2 19.463087248322147 24.65 3 8.251085669166995 15.675 4 2.9609159099881563 7.5 5 1.421239636794315 4.5 6 0.5132254243979472 1.95 7 0.2763521515988946 1.225 8 0.03947887879984208 0.2 9 0.07895775759968417 0.44999999999999996 >10 0.19739439399921044 1.55 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 15 0.375 No Hit GCCACGTTGATGAAAGGTCCCATAGCTTTGGCAGTTTGACCAAAAAAAAA 14 0.35000000000000003 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 12 0.3 No Hit AATGGTTCCAGGTGATGTTTCTGTGCCGCAGGCTGTTGCCTTCACAGGAG 11 0.27499999999999997 No Hit GTTTAGGCCTCTGAAATTTTTTATTTAGTTGAATACAGAAAATACGATAT 10 0.25 No Hit AGGAAATTCTAGCTGATGAGGTACTGCGAGACTTTTTTTCAGAGGAAACT 9 0.22499999999999998 No Hit GAAACTCCCAGACTCTCTTTTGAATGATATAAAGAAGAAGTATGAGGAAA 9 0.22499999999999998 No Hit GGTAAGTCCAAGAAGACCAAACGTACAGCTCCGAAGTCGGATGATATCTA 8 0.2 No Hit ATTCGCTAAAAATCTAAACCTCCTCTCCCTCTCTCCAAATCTAAAATACC 7 0.17500000000000002 No Hit GCTATTTCATCTTCCTTCTTCAGACCCGACCACAGCGCCACCCTCACTCT 7 0.17500000000000002 No Hit GGACACCAGAGGATATCGAAATGACTCTTCTGGAAGGGATCGCCTTAGAG 7 0.17500000000000002 No Hit CAAATCCCATGAGCATACTGAAACTTGCAAGCTTTTTCCCACCTCAACAG 7 0.17500000000000002 No Hit AGGTCTAAGAGACCTAAAAGTCCGGAGAGGAGAGAGGAGGCACCTCCTGT 7 0.17500000000000002 No Hit CGTCAAGTCAGCAACGAGATTAAAAATATCTCACAAAACAAACGTTAGCT 7 0.17500000000000002 No Hit GATATGCCCCTGTCCTTGACTGTCACACCTGCCACATTGCTGTCAAGTTT 7 0.17500000000000002 No Hit ATGAACTGCTTGCATGCCAGTACACCAACAATAGTTCATCGGGATTTGAA 6 0.15 No Hit AGAAATCAGTAGAATTAATGGTAGAGCGTATAGTTCCATATTCTCCTGCC 6 0.15 No Hit CTCTATAATTTTTTTATCAATCTTACAGCACAAGCCCTCCCTCCGTTCCC 6 0.15 No Hit ATGCTATTTCATGAAAATTCCTCTGCATGCTAATAATGTTTTCACCTTTG 6 0.15 No Hit CAGTGAGAGAGAAAGAGGGACTAAAATGGGTTGCTTTTCTTGTGCTGGAA 6 0.15 No Hit GCAATGGGTCTTGCAGTTTCCGAAGAAGCAGGCACTTCTGTGGAAACTTC 6 0.15 No Hit GCAACAAGAGCTTGTCAAAATCCATATCCTGTTATTGATGGAAGGAGAGC 6 0.15 No Hit CCCGCGAACATCGTCAAGGTTTCTGGCTCGCGCTCTGGTTGCGGCAAGGC 6 0.15 No Hit CTGTGATCCCGATTTAAGGTTAACTGGTCATGATAAAGAAGGGTATGGAT 6 0.15 No Hit GGCGAGTAGATGAGGGGTTTAGTGAAACGAGATTGTTGACGGAACTATCA 6 0.15 No Hit CTCAACTTCTCAAATCCTCATATCTCAAAGAATTTTGATCCCCGTGCTTG 6 0.15 No Hit GAAAGATGAGAAAGCTAAGAAGTCTGTCAGCATTAACGAGTTCTTGAAGC 6 0.15 No Hit CATGGTTCGCCAGCTTATTTCAAATGTCGGTCAAGCCTCTGGGCCTCCCA 6 0.15 No Hit GGGACGTTGAGTCTGTGAGGATCCGGTCCGTGCCAATTGCTGAATCCAAG 5 0.125 No Hit GTCACAGATCTACTCCCAAAGCAACATATATGAAGCAGGGCAGAAGAAGA 5 0.125 No Hit CCGATCTCGGCAAGAGAATGTTGCTGGTGTTAAGCTTCCTAAGTTCGACT 5 0.125 No Hit GTTCAATACAAACAGTTCAAGTGGACACTGTTGAAGAAAGCGGCTCAAGT 5 0.125 No Hit GATAAACTTCTAGTAATGCAGCAACAAGAGCTAGAGGAACTAAAGAGGAA 5 0.125 No Hit GTATCCGTTCAACCTTGATAGACTGAATTTCCTTAAAGAGCAAGAAGAGA 5 0.125 No Hit AGGGCTTTCAACAAATACTCAAAGCAGTATGAGACAGATGAAGGGAAGAA 5 0.125 No Hit GCTCATTGGATCATCGTTAAAACCTACCTGTCAGCTACTTGAATCTGATA 5 0.125 No Hit GGTGTCATGAACTCAAAGTCCTGGTTGATATGGAGAATTTATTGAGGTGG 5 0.125 No Hit TGAGGAACCTTCGGATGCAAAATTCTCTCAAAATAGTGCTGCACTGGTGT 5 0.125 No Hit CGTTACTTCACAAGCAGCAATTCTGGTAAGTGCAAGAGTGGCCTGAAATT 5 0.125 No Hit AGCTTGGTCAGCATGCCCAGCTGAAGTTTCCAGAGCTCAATCCATTGCTG 5 0.125 No Hit TGGGAGGGACCAATTCACAAGGTCAATGTATTTCGTCTGAATCCGTCATG 5 0.125 No Hit TCCATCTGGAAGCAGATGTCTGATGCTGGAATCAAGTTTATTCCTAGCAA 5 0.125 No Hit GGTTTCAATGGTCAGCCTGTTTCTAGTTTGTGCTTATATCTATCCACCGC 5 0.125 No Hit ATTGAGGCTGGATGATGTGGCAAATACATTGAAATGCTGGGGAGCTGTTT 5 0.125 No Hit CCCTCTTCTGCACTCATTGATTCGGCTGTGGAGACACTGTCTCACCTTTT 5 0.125 No Hit GCGTAGCAAGTGGTTCCTTTGGTCTTGATGCTGCAAAATTTGGATTAGAA 5 0.125 No Hit AATAACCCAACTATTATTCTCTGCTGATTCAGATTTCAATTTACAAATGG 5 0.125 No Hit GCTGCTGTTAGGCAGCTAAGTTCACGCAATCTGCTGCCGCTGCTGCCTAT 5 0.125 No Hit CCAAAAATTAACAGTAAAGAAAGAAAGAAAGAGAGAGAGACGGAGCAGAG 5 0.125 No Hit TTCATGGGAATGCTTGAATGGACCCTTCCTGCAGGTTGGAACCTGGATAA 5 0.125 No Hit GTCTACCAGCTTTTCCAATGGGTAAAATTGATCAGACACGCATGTGGAAG 5 0.125 No Hit ACAAGATCTACATCTTTAAGGTCCTGAAACAAGTTCATCCAGATATTGGG 5 0.125 No Hit CGGGGGCTGAGAGGAAGGAAGCTGCTGAGAGCACTTTGTTGGCTTACAAG 5 0.125 No Hit AAAAGAGGAGGTGTCAATGGAAAAGGTATTGAATGAGCTAGAAAACTTGG 5 0.125 No Hit GCTGGAGCCATTGTGTCTGGGGGAAAGGGCACTGCTCAAGACAAAATCAA 5 0.125 No Hit GTATCCTGACCGCATGATGTTGACATTTTCTGTTTTCCCTTCTCCTAAGG 5 0.125 No Hit CATCTTTTTGTGGAGGTTCTTCTTTTAGTGGTCATTCTTTTTCTTCTTTC 5 0.125 No Hit GGAATATCAGCAAAAATATTCTGGGGGTCGTTGGGCTGATGAATATGCAC 5 0.125 No Hit TGCTACCAAACCTTGTAAACCTAGAAAAGATTACAGTTAGAGATTGTGAG 5 0.125 No Hit GCTCTGAATGTCAAAGTGAAGAAATTCAACCAAGCGCGGGTAAACGGCGG 5 0.125 No Hit CTTATTATTAGATCTTAGCCTTCGGGACAATTTCATTTGTTAGCTAGCGT 5 0.125 No Hit AATGTGTTGGTGGTACTTCTTCAGCTATGGTACACAAAAACGGTACTTCT 5 0.125 No Hit CACAGGGCTGGTAAAATTGTGGTTGAATTGAATGGAAGATTGAACAAATG 5 0.125 No Hit CGACCAGACATCGTCAACTACGTCCACTCAAACATCTCCAAGAATAGCCG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0125 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.037500000000000006 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.075 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.125 0.0 0.0 0.0 0.0 66-67 0.15 0.0 0.0 0.0 0.0 68-69 0.15 0.0 0.0 0.0 0.0 70-71 0.1875 0.0 0.0 0.0 0.0 72-73 0.225 0.0 0.0 0.0 0.0 74-75 0.275 0.0 0.0 0.0 0.0 76-77 0.32499999999999996 0.0 0.0 0.0 0.0 78-79 0.35 0.0 0.0 0.0 0.0 80-81 0.375 0.0 0.0 0.0 0.0 82-83 0.375 0.0 0.0 0.0 0.0 84-85 0.5125 0.0 0.0 0.0 0.0 86-87 0.7125 0.0 0.0 0.0 0.0 88-89 0.9750000000000001 0.0 0.0 0.0 0.0 90-91 1.1375 0.0 0.0 0.0 0.0 92-93 1.2625 0.0 0.0 0.0 0.0 94-95 1.5125 0.0 0.0 0.0 0.0 96-97 1.6875 0.0 0.0 0.0 0.0 98-99 1.875 0.0 0.0 0.0 0.0 100-101 2.2375 0.0 0.0 0.0 0.0 102-103 2.525 0.0 0.0 0.0 0.0 104-105 2.8875 0.0 0.0 0.0 0.0 106-107 3.5374999999999996 0.0 0.0 0.0 0.0 108-109 3.9 0.0 0.0 0.0 0.0 110-111 4.2 0.0 0.0 0.0 0.0 112-113 4.7875 0.0 0.0 0.0 0.0 114-115 5.1875 0.0 0.0 0.0 0.0 116-117 5.65 0.0 0.0 0.0 0.0 118-119 6.2375 0.0 0.0 0.0 0.0 120-121 6.800000000000001 0.0 0.0 0.0 0.0 122-123 7.2875 0.0 0.0 0.0 0.0 124-125 7.7 0.0 0.0 0.0 0.0 126-127 8.2625 0.0 0.0 0.0 0.0 128-129 9.0 0.0 0.0 0.0 0.0 130-131 9.9 0.0 0.0 0.0 0.0 132-133 10.375 0.0 0.0 0.0 0.0 134-135 11.0375 0.0 0.0 0.0 0.0 136-137 11.7375 0.0 0.0 0.0 0.0 138-139 12.4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCGTA 10 0.006830828 145.0 5 TCGTAAT 10 0.006830828 145.0 7 AATATCG 10 0.006830828 145.0 3 ATCGTAA 10 0.006830828 145.0 6 ATATCGT 10 0.006830828 145.0 4 TTTATTT 20 0.00593511 29.0 45-49 >>END_MODULE Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256587 spots for SRR26075342.sra Written 2256587 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra Read 2256571 spots for SRR26075342.sra Written 2256571 spots for SRR26075342.sra SRR ids: ['SRR26075342.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_95g8rz4k SRR26075342.sra spots: 45131436 blocks: [[1, 2256571], [2256572, 4513142], [4513143, 6769713], [6769714, 9026284], [9026285, 11282855], [11282856, 13539426], [13539427, 15795997], [15795998, 18052568], [18052569, 20309139], [20309140, 22565710], [22565711, 24822281], [24822282, 27078852], [27078853, 29335423], [29335424, 31591994], [31591995, 33848565], [33848566, 36105136], [36105137, 38361707], [38361708, 40618278], [40618279, 42874849], [42874850, 45131436]] SRR26075342 file size 16669501 SRR26075342 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075342 SRR26075342_1.fastq SRR26075342_2.fastq Input file: SRR26075342_1.fastq Paired file: SRR26075342_2.fastq trimmed: SRR26075342-trimmed-pair1.fastq, SRR26075342-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 21:17:18 2025 >> started Tue Feb 11 21:18:11 2025 >> done (52.779s) 45131436 read pairs processed; of these: 178 ( 0.00%) short read pairs filtered out after trimming by size control 182602 ( 0.40%) empty read pairs filtered out after trimming by size control 44948656 (99.60%) read pairs available; of these: 7487665 (16.66%) trimmed read pairs available after processing 37460991 (83.34%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 13 0.00% 20 19 0.00% 21 24 0.00% 22 26 0.00% 23 31 0.00% 24 38 0.00% 25 24 0.00% 26 33 0.00% 27 52 0.00% 28 48 0.00% 29 44 0.00% 30 51 0.00% 31 54 0.00% 32 79 0.00% 33 49 0.00% 34 74 0.00% 35 66 0.00% 36 80 0.00% 37 77 0.00% 38 111 0.00% 39 93 0.00% 40 134 0.00% 41 128 0.00% 42 135 0.00% 43 137 0.00% 44 175 0.00% 45 166 0.00% 46 183 0.00% 47 201 0.00% 48 235 0.00% 49 262 0.00% 50 355 0.00% 51 343 0.00% 52 381 0.00% 53 423 0.00% 54 408 0.00% 55 521 0.00% 56 635 0.00% 57 606 0.00% 58 846 0.00% 59 887 0.00% 60 1048 0.00% 61 1218 0.00% 62 1376 0.00% 63 1670 0.00% 64 1861 0.00% 65 2032 0.00% 66 2263 0.01% 67 2615 0.01% 68 2824 0.01% 69 3206 0.01% 70 3878 0.01% 71 4577 0.01% 72 5004 0.01% 73 6135 0.01% 74 6638 0.01% 75 7669 0.02% 76 8524 0.02% 77 9560 0.02% 78 10434 0.02% 79 11590 0.03% 80 13058 0.03% 81 14511 0.03% 82 16519 0.04% 83 18109 0.04% 84 20434 0.05% 85 22668 0.05% 86 24534 0.05% 87 26204 0.06% 88 28427 0.06% 89 30260 0.07% 90 31429 0.07% 91 34060 0.08% 92 37229 0.08% 93 40576 0.09% 94 44157 0.10% 95 47091 0.10% 96 49220 0.11% 97 52957 0.12% 98 54558 0.12% 99 56610 0.13% 100 59369 0.13% 101 61261 0.14% 102 63891 0.14% 103 67661 0.15% 104 72179 0.16% 105 75334 0.17% 106 80066 0.18% 107 82254 0.18% 108 84065 0.19% 109 86415 0.19% 110 87545 0.19% 111 90221 0.20% 112 93172 0.21% 113 95277 0.21% 114 100024 0.22% 115 105117 0.23% 116 108549 0.24% 117 111592 0.25% 118 116299 0.26% 119 115436 0.26% 120 119131 0.27% 121 120424 0.27% 122 121306 0.27% 123 125559 0.28% 124 130322 0.29% 125 134329 0.30% 126 137378 0.31% 127 142631 0.32% 128 144645 0.32% 129 146553 0.33% 130 149096 0.33% 131 148941 0.33% 132 151360 0.34% 133 154215 0.34% 134 156868 0.35% 135 160512 0.36% 136 164299 0.37% 137 168321 0.37% 138 170524 0.38% 139 174648 0.39% 140 177682 0.40% 141 176764 0.39% 142 179659 0.40% 143 178434 0.40% 144 181513 0.40% 145 185465 0.41% 146 185303 0.41% 147 190725 0.42% 148 195343 0.43% 149 195946 0.44% 150 199056 0.44% 151 37460991 83.34% 44948656 reads passed initial QC criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=2.42 fanout-score-rank=40 prefix-density=0.27 prefix-fanout=2.4 sequence=GTCAGGGTACAT criterion=fanout-score sequence-density=0.01 sequence-density-rank=43 fanout-score=384.17 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=14.2 sequence=TTTTCTTGACATGCTATATCCAAACAACCCTGAAAGAAGTTCTGCCAACACAAGAAAGACGGGCATTTTATTTAAATATCTGGTGTTGGTTCACACTACAGCTGGAATGTAGTTTCCCAGTAATCTAAGCACAACCGACATGCTCCCTAGCCTATGGAAATTTGAAGGTCTTCCATTTGCACAGTTCAAAGCCCTTTTGCTTCTGCTGGATGGAGCCATCAGCTTCACCGTTAACCTCTTCTTCTTGAGATACCTTGTTGTTGAAGGCCTTGTGAACACTTGGGTACTGGGGGGCACCATGGATAACGCTAGGCATCTTTGATAACGTCGAAATATAGTCATGGTAATCACCGGACGTGGTGTTCGAGCCTTGCCGCTGCCTAGTTTGGTAGGCAGAGCCTTGAGACATTTTTTGCT criterion=sequence-density sequence-density=0.48 sequence-density-rank=1 fanout-score=2.49 fanout-score-rank=34 prefix-density=0.49 prefix-fanout=2.4 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=170.13 fanout-score-rank=1 prefix-density=0.16 prefix-fanout=5.5 sequence=CAAAGCAGTTGCATTTATCTAAAGTATTCTCACTTTACTTAACACTTGAGCTACATAGAATGTCTACAGTCAATTTGGCAGCACTGCTGTTGCTTGGGCTGCTGCTGGTTATGCCACAGCAGTCCATGCAAGCGAGTTTGATAGACCCCATTGCTGAAATCGAGAGAAGCAACTGCAAAATCGCACACCTTCGCTTAGGGCTTGTTTTTAC SRR26075342 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 21:18:57 Started mapping on | Feb 11 21:18:57 Finished on | Feb 11 21:25:20 Mapping speed, Million of reads per hour | 422.49 Number of input reads | 44948656 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 40955485 Uniquely mapped reads % | 91.12% Average mapped length | 291.98 Number of splices: Total | 40322822 Number of splices: Annotated (sjdb) | 39256078 Number of splices: GT/AG | 39533479 Number of splices: GC/AG | 602361 Number of splices: AT/AC | 47141 Number of splices: Non-canonical | 139841 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 3.32 Insertion rate per base | 0.02% Insertion average length | 2.67 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1135205 % of reads mapped to multiple loci | 2.53% Number of reads mapped to too many loci | 284294 % of reads mapped to too many loci | 0.63% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.38% % of reads unmapped: other | 0.35% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2857966 2857966 2857966 N_multimapping 1135205 1135205 1135205 N_noFeature 1084986 40466049 1400193 N_ambiguous 402138 2334 226256 UnstrandedReadsAssigned:39468361 PositiveStrandReadsAssigned:487102 NegativeStrandReadsAssigned:39329036 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075342 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075342-trimmed-pair1.fastq SRR26075342-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 44,948,656 reads, 39,886,348 reads pseudoaligned [quant] estimated average fragment length: 211.894 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,288 rounds 52401 SRR26075342.ke.tsv 34699 SRR26075342.se.tsv 87100 total ==> SRR26075342.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1807.11 4365 54.9911 Potri.005G024800.1.v4.1 1035 824.106 3491 96.4403 Potri.004G059700.1.v4.1 961 750.128 0 0 Potri.007G009000.2.v4.1 1416 1205.11 0 0 Potri.003G141000.2.v4.1 2943 2732.11 1962 16.3491 Potri.016G087400.1.v4.1 270 92.2821 3813.56 940.816 Potri.015G069301.1.v4.1 564 355.298 0 0 Potri.010G195200.1.v4.1 1773 1562.11 626.68 9.13329 Potri.012G127500.1.v4.1 977 766.128 12797 380.276 ==> SRR26075342.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 37 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 501 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 11 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 3824 SRR26075342 completed mapping pipeline successfully