Starting /dee2/code/volunteer_pipeline.sh SRR26075343 current disk space = 3052470669312 free memory = 1572504828 SRR26075343 SRAfilesize 1b4d49c9a47da939bcc986879ce480b4 SRR26075343.sra SRR26075343.sra file validated SRR26075343 is paired end SRR26075343 is conventional basespace SRR26075343 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075343_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.656 37.0 37.0 37.0 37.0 37.0 2 36.555 37.0 37.0 37.0 37.0 37.0 3 36.5505 37.0 37.0 37.0 37.0 37.0 4 36.6775 37.0 37.0 37.0 37.0 37.0 5 36.669 37.0 37.0 37.0 37.0 37.0 6 36.685 37.0 37.0 37.0 37.0 37.0 7 36.6135 37.0 37.0 37.0 37.0 37.0 8 36.6435 37.0 37.0 37.0 37.0 37.0 9 36.649 37.0 37.0 37.0 37.0 37.0 10-14 36.6243 37.0 37.0 37.0 37.0 37.0 15-19 36.5847 37.0 37.0 37.0 37.0 37.0 20-24 36.543099999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.45360000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.406600000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.3755 37.0 37.0 37.0 37.0 37.0 40-44 36.3232 37.0 37.0 37.0 37.0 37.0 45-49 36.3082 37.0 37.0 37.0 37.0 37.0 50-54 36.2433 37.0 37.0 37.0 37.0 37.0 55-59 36.1974 37.0 37.0 37.0 37.0 37.0 60-64 36.1496 37.0 37.0 37.0 37.0 37.0 65-69 36.11750000000001 37.0 37.0 37.0 37.0 37.0 70-74 36.1682 37.0 37.0 37.0 37.0 37.0 75-79 36.0711 37.0 37.0 37.0 37.0 37.0 80-84 35.9674 37.0 37.0 37.0 37.0 37.0 85-89 35.905499999999996 37.0 37.0 37.0 37.0 37.0 90-94 35.8323 37.0 37.0 37.0 37.0 37.0 95-99 35.9461 37.0 37.0 37.0 37.0 37.0 100-104 35.810199999999995 37.0 37.0 37.0 37.0 37.0 105-109 35.7713 37.0 37.0 37.0 37.0 37.0 110-114 35.7062 37.0 37.0 37.0 37.0 37.0 115-119 35.590999999999994 37.0 37.0 37.0 37.0 37.0 120-124 35.633799999999994 37.0 37.0 37.0 37.0 37.0 125-129 35.3781 37.0 37.0 37.0 34.6 37.0 130-134 35.324200000000005 37.0 37.0 37.0 32.2 37.0 135-139 35.1734 37.0 37.0 37.0 29.8 37.0 140-144 35.0621 37.0 37.0 37.0 29.8 37.0 145-149 35.0429 37.0 37.0 37.0 25.0 37.0 150-151 34.901250000000005 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 2.0 22 3.0 23 0.0 24 5.0 25 4.0 26 6.0 27 8.0 28 19.0 29 31.0 30 38.0 31 46.0 32 60.0 33 109.0 34 184.0 35 460.0 36 2835.0 37 189.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.3973973973974 13.113113113113112 6.606606606606606 32.88288288288289 2 19.25 13.3 34.4 33.050000000000004 3 18.2 18.55 30.875000000000004 32.375 4 24.474999999999998 23.674999999999997 25.275 26.575 5 26.1 27.775 24.224999999999998 21.9 6 23.425 33.725 22.075 20.775 7 16.225 27.650000000000002 39.95 16.175 8 18.125 27.6 30.8 23.474999999999998 9 19.2 21.525 36.525 22.75 10-14 20.385 28.82 26.55 24.245 15-19 20.935000000000002 26.855 28.405 23.805 20-24 21.16 27.72 27.74 23.380000000000003 25-29 20.105 27.92 28.455000000000002 23.52 30-34 20.72 26.97 28.105000000000004 24.205 35-39 20.87 27.46 27.955000000000002 23.715 40-44 20.525 28.255000000000003 27.395000000000003 23.825 45-49 21.13 27.845 27.52 23.505000000000003 50-54 20.745 27.63 26.939999999999998 24.685000000000002 55-59 20.905 27.1 28.215 23.78 60-64 20.97 27.355 27.544999999999998 24.13 65-69 20.135 28.24 27.87 23.755000000000003 70-74 20.669999999999998 27.235 27.395000000000003 24.7 75-79 21.055 27.73 27.33 23.885 80-84 21.279999999999998 27.125 27.99 23.605 85-89 20.974999999999998 27.894999999999996 27.185 23.945 90-94 20.395 27.41 28.205000000000002 23.990000000000002 95-99 20.635 27.644999999999996 27.529999999999998 24.19 100-104 21.21 27.565 27.62 23.605 105-109 21.634999999999998 26.695 28.15 23.52 110-114 21.83 27.705000000000002 27.525 22.939999999999998 115-119 21.91 27.205000000000002 27.305 23.580000000000002 120-124 20.974999999999998 27.63 27.060000000000002 24.335 125-129 21.775 27.38 27.005000000000003 23.84 130-134 22.1 27.675 26.82 23.405 135-139 21.65 27.36 26.900000000000002 24.09 140-144 21.675 27.139999999999997 27.41 23.775 145-149 22.040000000000003 27.205000000000002 26.784999999999997 23.97 150-151 22.4625 27.975 25.112499999999997 24.45 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 1.0 23 3.0 24 3.0 25 1.0 26 1.5 27 6.5 28 8.5 29 11.0 30 17.0 31 26.0 32 31.0 33 36.0 34 46.5 35 57.5 36 75.0 37 78.5 38 99.5 39 141.0 40 151.0 41 195.0 42 240.5 43 244.0 44 289.5 45 296.5 46 256.0 47 245.5 48 240.0 49 215.5 50 188.0 51 154.0 52 114.5 53 83.0 54 69.0 55 69.0 56 62.5 57 49.0 58 29.5 59 27.0 60 31.5 61 25.5 62 18.0 63 14.0 64 11.5 65 6.5 66 3.0 67 6.0 68 5.5 69 5.0 70 4.5 71 3.0 72 1.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.025 #Duplication Level Percentage of deduplicated Percentage of total 1 63.361547762998796 39.300000000000004 2 21.96694881096332 27.250000000000004 3 8.94800483675937 16.650000000000002 4 3.1035872632003225 7.7 5 1.7734784361144702 5.5 6 0.5239822652156388 1.95 7 0.12091898428053204 0.525 8 0.08061265618702136 0.4 9 0.08061265618702136 0.44999999999999996 >10 0.04030632809351068 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTTTCTCTGCACTTTCCCTTGAAGGCCACTGGTCCATAGGACGAATGAC 11 0.27499999999999997 No Hit CTCTTGACCAACAAAGAGTGGACATATTGAACCGTTGTTAAGATTAACAA 9 0.22499999999999998 No Hit GTTTTGTATGCCTCAGGCTGGCCAGGTAACCTACCCAACTTATACCTAAA 9 0.22499999999999998 No Hit CAGGTCTCTGTGATGGGGAAATCAAAAACCACATCTTTCCCTGTAAGCTT 8 0.2 No Hit GTACGGTTCTTTCTCCTCCGCTGACATAGCATTCCACTTGTCCGCAAGTG 8 0.2 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCGATACATCGCGTAT 7 0.17500000000000002 TruSeq Adapter, Index 12 (97% over 37bp) GCTGGAAGTAGGGAGGAGGATATTGGTAGTGCTGAGGACCATATAATTGA 7 0.17500000000000002 No Hit CCCCATTCGAAAGCTTTCATGGGTTCGATGAGATTGACGAGATTAGCATA 7 0.17500000000000002 No Hit AGAGGAGGCTGCAAGGCAGGCCTGGACATTGAGGCCGAAGAAGAGAACGG 6 0.15 No Hit CTAGATAGTCTCTTCACGGCTATATATTGTCCGTTGGGAAGGGTACCCTT 6 0.15 No Hit GCTCAAAATGCTTTGGTGGTTGGCGGAGCAGGTAAAGCATGGATTTCCAG 6 0.15 No Hit GCCAGATTTCTTCACGTCGCCGAAATTCACCTGAAGAGAAACCACTTGTC 6 0.15 No Hit CCTCCCACTTGTTCTTCACATCAGCCTTCTCCATGGCTTCAATTAGTGCC 6 0.15 No Hit ACCACGCGGAACTTAAAGCGGTAAAAATGTATGAAAAATAGCAGCAGCAG 6 0.15 No Hit GGAGGCAAACCACGCAATATGGGGTCTCGTAATCATCGTTCTTGATGGGA 6 0.15 No Hit CTCGGCAATAGGATTGTAGCCCAGGATGAGTCCCTCAGCGTGACGCAGTA 6 0.15 No Hit GCACCGACATCGTGTCCTTAATATTTTTGCCCTCGCTTTGGAACTCAAGG 6 0.15 No Hit ATTCGTATTCTCTATTTTTATCTTCGCGTTCCGACCGCTGCCAGCGGCGG 6 0.15 No Hit GGCGCTTTTGGAGGGGATCCACGAGATACTGCTCCTCAAGTTTATGCTCT 6 0.15 No Hit GAGGGGAATCTTCAGTTTTCTTATGCTGGTATCATGTCCTTGGCTTGCTC 6 0.15 No Hit GGGGTCCAAGACAAGGGAGCTTGAAAGGCTGAATCTTGAGAGTATTGACA 6 0.15 No Hit CTCATATGCTGCTCTTTCTGGATCCACTAAAGAACCTTGAGGTTGACTAT 5 0.125 No Hit CGCCAAACGAAGCTCATTCAAGCTCTTCCAACCCTGAATTCCGGATGGAA 5 0.125 No Hit GCCGTAATAACCATCATGGCTTGGCGCTATTGAGTTTAACTGTCCCAGGC 5 0.125 No Hit CTTTGATTAGGCCTAAGAGTCAGAGTACAAGTTGAGAAAAAGGCACACAA 5 0.125 No Hit CAGAAGTATTAGCCACGCAGAGGTCTTGAAGCATGTCAGGATCGTAAGCA 5 0.125 No Hit GGCGTGGCTTTCGGATCGGATCTTGGTAATTCGGGTAGAGTGAAGTCCCC 5 0.125 No Hit GCGAGAGAGACTGTCGAGTTGGAATTGGCGGTTGGAAGGGAGTTCAACAC 5 0.125 No Hit GATTCTTCTCGGCAAAGCCAGATGGCAAATACATAATGCATCCCCTGTAT 5 0.125 No Hit TCTCAGTGCGCATCGGAGTAACCCTAACTTCAACACCAGAGTATCCATCC 5 0.125 No Hit CTACTGTCTCACTATCTCCCATACTCTACAACTTGTACTGCTACAGTATA 5 0.125 No Hit GGATAATGTTTGTGCAGACACCATTCAAGAAATCCTGAGAGTGAGAAACC 5 0.125 No Hit GGTCGGATCTGCAAACTGTATGGGGGGAGGCTATGACCTGCACCTATATA 5 0.125 No Hit GTTTGGTGGATATCGGTATGGCAAACTCCACAGCTGATAACCTTGATAAA 5 0.125 No Hit CAGGCCATGGCTAGCTAACTGTACTTTAATTTACAGCAAATACTATATTA 5 0.125 No Hit CCTTGATTCCCGCAAAGCTGCAAAACTAATTCAGGGCTTCCATCACAACA 5 0.125 No Hit ACCGAGTAAAAAACCGAAGAACGTGACTTTTCCAGGACTACCTGTCGTCT 5 0.125 No Hit ATTCGGTAGCTCAAGCTCAGCTGAGTTGAGTTTTCATTGATTCGGTAGCT 5 0.125 No Hit GCAAGGGAAAAGATTGTGTTCCTTAATTTCTTATGACAGCTGTATGAATT 5 0.125 No Hit GACTAGGAGACTTGTCTCCATTTACTTCTTTAGGATATTCATCATGAATT 5 0.125 No Hit CCTGTACTGTTTGATGACATCACCTACAGTCTTGCCTGGAATCATGGCTG 5 0.125 No Hit GTCGTCACTCCATTTGAAGAATCCGCAACTACCCTAAAAATTAACAACAA 5 0.125 No Hit CCTGATTCTCTTTCTCTGTCTTCTCTTTCTCCTGCTTTACCTTCAGTTTC 5 0.125 No Hit GCTCCACTCCTCCAACACGGGCACATCGGATGCTTAGACTGCCAGCCTAA 5 0.125 No Hit AGTGAAATCAGCGATTCGGCCGAGCTTTTCAGAGCGAGAGAAGGGAGCGA 5 0.125 No Hit GCCCATGCCTTCATCACAAAGGGGTCGTTGACGCTGATGCACAAAATTTC 5 0.125 No Hit CTGTTCTCAATCATGTACTTCTTCAAGGCTGCGATGGGATCTCTAGCAGC 5 0.125 No Hit CCTATGGATACGCTTATTCATATATTGCCAGGCTTAGCTTCGATCGAGTT 5 0.125 No Hit CTTGTCAATCCGTCGATGCGATGATCATTTCTTGAATCAACGCAGCCAGC 5 0.125 No Hit TGGTGGAGGAGGAATCAAGCAATTGAAGCGCCTTTCTGGAACTGGGCAGT 5 0.125 No Hit GTACATTTTGAAAAGAAACTGTGAGACACGAATTTACAGAACAGTGTTTA 5 0.125 No Hit GTTTGATTTTGAGCGGAAAAGGGAAGAGGGCAAGCGAGATAAAGGGGAAT 5 0.125 No Hit GGGTGACAAGCTACTAAAGATGCGCTTTAAACTCGGGCAGGCTGCACGTA 5 0.125 No Hit CCTGTGTGCATAACTTTAACAGCTTTCAGTGGGAATTTTCCATGTGCAGT 5 0.125 No Hit GAATGATTTAAAAATGGCCAAAAGCGGGTTGGTTTCTGGTTCTGGTCGAT 5 0.125 No Hit CTCCCTTCTTGAAGAGTTGGACAGAGATTTTCTATAAGAACAAGCAAGAA 5 0.125 No Hit CTTGTACATATGGGCAATCTCTGGTACAAGAGGGTCATCAGGATTTGGAT 5 0.125 No Hit CGCTGAGGATGATTCCAGGGTGAAGAATTGTCCTAGACATGTCTCAAAGG 5 0.125 No Hit ATGGGACTCTTTGCCCTGCTCTGAACAGAAGAAAAGGAGGCGATCAGCAT 5 0.125 No Hit GCCAGGGGGCTTGCGGACACCACCAAGATAATCTCTGGATGCTGCTTTTC 5 0.125 No Hit CCCACTGGGACAGTTCCGATACCACCAATCTTGTACACGTCCTGAAGCGG 5 0.125 No Hit GCTTATGATTTCCGTATGGTGTTGCAGAAAGTTCTGCAGGTCCAACTAAA 5 0.125 No Hit CAGGTCTTTGATCCTGCTGGCAAATTACACATTTTACTTATGGTTCCACT 5 0.125 No Hit GCCTCCTCTCAAACGGAGCACCAAGTGGAGGGTGGACTCCTTCTGGATAT 5 0.125 No Hit GCTTTGCCAGGCCAAAATCAGTCAACATCACATGGCCATCTGAATCCAGC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0125 0.0 0.0 0.0 0.0 66-67 0.037500000000000006 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.0875 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.16249999999999998 0.0 0.0 0.0 0.0 78-79 0.21250000000000002 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.25 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.3375 0.0 0.0 0.0 0.0 90-91 0.4375 0.0 0.0 0.0 0.0 92-93 0.55 0.0 0.0 0.0 0.0 94-95 0.6000000000000001 0.0 0.0 0.0 0.0 96-97 0.8125 0.0 0.0 0.0 0.0 98-99 0.95 0.0 0.0 0.0 0.0 100-101 1.1875 0.0 0.0 0.0 0.0 102-103 1.5 0.0 0.0 0.0 0.0 104-105 1.9 0.0 0.0 0.0 0.0 106-107 2.0875000000000004 0.0 0.0 0.0 0.0 108-109 2.45 0.0 0.0 0.0 0.0 110-111 2.6375 0.0 0.0 0.0 0.0 112-113 3.025 0.0 0.0 0.0 0.0 114-115 3.3625 0.0 0.0 0.0 0.0 116-117 3.55 0.0 0.0 0.0 0.0 118-119 3.95 0.0 0.0 0.0 0.0 120-121 4.275 0.0 0.0 0.0 0.0 122-123 4.7125 0.0 0.0 0.0 0.0 124-125 5.112500000000001 0.0 0.0 0.0 0.0 126-127 5.8 0.0 0.0 0.0 0.0 128-129 6.2375 0.0 0.0 0.0 0.0 130-131 6.574999999999999 0.0 0.0 0.0 0.0 132-133 7.275 0.0 0.0 0.0 0.0 134-135 7.7125 0.0 0.0 0.0 0.0 136-137 8.575 0.0 0.0 0.0 0.0 138-139 9.1625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCAGA 10 0.006830828 145.0 1 ATTTGAT 10 0.006830828 145.0 5 TTCAGAA 10 0.006830828 145.0 2 >>END_MODULE SRR26075343 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075343_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.415 37.0 37.0 37.0 37.0 37.0 2 36.203 37.0 37.0 37.0 37.0 37.0 3 36.21 37.0 37.0 37.0 37.0 37.0 4 36.2465 37.0 37.0 37.0 37.0 37.0 5 36.276 37.0 37.0 37.0 37.0 37.0 6 36.1525 37.0 37.0 37.0 37.0 37.0 7 36.207 37.0 37.0 37.0 37.0 37.0 8 36.1625 37.0 37.0 37.0 37.0 37.0 9 36.244 37.0 37.0 37.0 37.0 37.0 10-14 36.145599999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.069900000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.0554 37.0 37.0 37.0 37.0 37.0 25-29 35.9206 37.0 37.0 37.0 37.0 37.0 30-34 35.836499999999994 37.0 37.0 37.0 37.0 37.0 35-39 35.8192 37.0 37.0 37.0 37.0 37.0 40-44 35.7176 37.0 37.0 37.0 37.0 37.0 45-49 35.645500000000006 37.0 37.0 37.0 37.0 37.0 50-54 35.5015 37.0 37.0 37.0 37.0 37.0 55-59 35.5809 37.0 37.0 37.0 37.0 37.0 60-64 35.5704 37.0 37.0 37.0 37.0 37.0 65-69 35.5097 37.0 37.0 37.0 37.0 37.0 70-74 35.3964 37.0 37.0 37.0 37.0 37.0 75-79 35.342699999999994 37.0 37.0 37.0 37.0 37.0 80-84 35.3368 37.0 37.0 37.0 37.0 37.0 85-89 35.337 37.0 37.0 37.0 37.0 37.0 90-94 35.141400000000004 37.0 37.0 37.0 32.2 37.0 95-99 35.24980000000001 37.0 37.0 37.0 37.0 37.0 100-104 35.185 37.0 37.0 37.0 32.2 37.0 105-109 35.1641 37.0 37.0 37.0 34.6 37.0 110-114 35.104000000000006 37.0 37.0 37.0 29.8 37.0 115-119 35.1374 37.0 37.0 37.0 32.2 37.0 120-124 34.929500000000004 37.0 37.0 37.0 25.0 37.0 125-129 34.938900000000004 37.0 37.0 37.0 25.0 37.0 130-134 34.9049 37.0 37.0 37.0 25.0 37.0 135-139 34.74915 37.0 37.0 37.0 25.0 37.0 140-144 34.734950000000005 37.0 37.0 37.0 25.0 37.0 145-149 34.7107 37.0 37.0 37.0 25.0 37.0 150-151 34.473749999999995 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 10.0 14 12.0 15 6.0 16 8.0 17 4.0 18 8.0 19 7.0 20 8.0 21 12.0 22 13.0 23 18.0 24 21.0 25 13.0 26 29.0 27 15.0 28 18.0 29 17.0 30 28.0 31 38.0 32 49.0 33 84.0 34 178.0 35 582.0 36 2561.0 37 260.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.775 22.75 8.725 19.75 2 30.725 25.25 26.025 18.0 3 24.625 27.525 30.825000000000003 17.025000000000002 4 28.725 31.85 20.775 18.65 5 26.825 36.9 20.25 16.025 6 23.1 38.574999999999996 22.575 15.75 7 23.75 23.625 33.475 19.15 8 24.25 25.35 26.5 23.9 9 23.425 23.925 28.875 23.775 10-14 25.679999999999996 29.78 24.224999999999998 20.315 15-19 25.365 28.785 25.4 20.45 20-24 24.490000000000002 28.994999999999997 26.105 20.41 25-29 24.490000000000002 28.705000000000002 25.82 20.985 30-34 24.38 28.575 26.669999999999998 20.375 35-39 25.09 28.605000000000004 25.509999999999998 20.794999999999998 40-44 24.875 28.82 25.655 20.65 45-49 25.03 29.015 26.165 19.79 50-54 23.74 28.294999999999998 27.644999999999996 20.32 55-59 24.45 28.105000000000004 26.810000000000002 20.635 60-64 24.89 28.275 26.490000000000002 20.345 65-69 26.135 27.505000000000003 25.935000000000002 20.424999999999997 70-74 24.435000000000002 28.92 25.619999999999997 21.025 75-79 24.81 28.16 26.150000000000002 20.880000000000003 80-84 24.925 28.1 26.305 20.669999999999998 85-89 24.285 27.939999999999998 27.025 20.75 90-94 24.610000000000003 28.415000000000003 26.479999999999997 20.495 95-99 24.89 28.555000000000003 26.215 20.34 100-104 25.130000000000003 28.599999999999998 25.965 20.305 105-109 24.985 27.205000000000002 27.82 19.99 110-114 25.069999999999997 27.82 26.365 20.745 115-119 25.56 28.549999999999997 26.224999999999998 19.665 120-124 25.995 28.685 25.465 19.855 125-129 25.53 28.22 26.815 19.435 130-134 25.665 28.64 25.96 19.735 135-139 25.546277313865694 28.656432821641083 26.02630131506575 19.77098854942747 140-144 26.66399959993999 28.00920138020703 25.388808321248185 19.93799069860479 145-149 26.380552220888354 28.551420568227293 25.430172068827535 19.63785514205682 150-151 26.431607901975497 28.94473618404601 25.506376594148538 19.117279319829958 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.5 10 0.5 11 2.5 12 4.5 13 3.5 14 4.5 15 3.5 16 1.0 17 1.0 18 1.0 19 2.0 20 2.0 21 2.0 22 1.5 23 1.5 24 2.0 25 2.0 26 3.5 27 3.0 28 7.0 29 10.5 30 9.0 31 11.5 32 17.0 33 29.5 34 35.5 35 44.5 36 70.0 37 80.5 38 114.0 39 151.5 40 172.0 41 229.0 42 257.0 43 264.5 44 262.0 45 277.5 46 301.5 47 265.0 48 214.5 49 186.5 50 169.0 51 155.0 52 121.0 53 81.5 54 69.5 55 53.0 56 43.0 57 36.0 58 25.5 59 20.5 60 17.5 61 19.0 62 18.5 63 12.5 64 8.0 65 4.0 66 2.0 67 2.0 68 2.5 69 3.5 70 3.0 71 2.5 72 3.5 73 2.0 74 1.0 75 0.5 76 1.0 77 1.5 78 1.0 79 1.5 80 1.0 81 0.0 82 0.5 83 0.5 84 1.5 85 3.5 86 3.5 87 2.0 88 1.5 89 1.5 90 1.0 91 1.5 92 1.5 93 2.0 94 2.0 95 1.5 96 1.5 97 1.5 98 2.0 99 3.5 100 17.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.005 140-144 0.015 145-149 0.04 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.64999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 65.68236233040702 41.15 2 20.43096568236233 25.6 3 8.260175578611333 15.525 4 3.192338387869114 8.0 5 1.5562649640861932 4.875 6 0.5187549880287311 1.95 7 0.07980845969672785 0.35000000000000003 8 0.03990422984836393 0.2 9 0.11971268954509177 0.675 >10 0.11971268954509177 1.675 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 46 1.15 No Hit TGATGGACCAAAGGTTCCATTTTTCAGTGATGAAGAAGAAACACCCAGCA 11 0.27499999999999997 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 10 0.25 No Hit GCTGAGATTGTTGGAAAGCGCACCAGATACAGGATTGATGGATCCAAAAT 9 0.22499999999999998 No Hit GGGGTATAAAGGATGTGGGAAATCCGACTGGAAAGGTATATATGTATGAT 9 0.22499999999999998 No Hit ATTACAAATTGCTCATAGCGTCTTAATTTTAAGAACCAGCACATATAATA 9 0.22499999999999998 No Hit GAGAAAACCAAAAAAGAAGAAGGATCCCAATGCACCGAAGAGATCAAAAT 8 0.2 No Hit GAGAGAGAGAGAGAGATACTGTTCTCATGGAAGAAGCAAAAGGAGTAGTG 7 0.17500000000000002 No Hit GGTAGATGCTCCAGATATGGTTAGGAGTCAAATGAACTTCAAGAGGCTTT 7 0.17500000000000002 No Hit AGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGGCGCACAAGC 6 0.15 No Hit ACTGGTGAATAGTGGAAGGCAAATCGATGCAGTAAACTTGGCTTTTGCTT 6 0.15 No Hit AGATGGAGTTGGTGATGAGAAAAGTAGCTAGTTGTATAGCAGCCACAGCA 6 0.15 No Hit GTCGGCGTTCAAAGAGGATATAGCAAACATGGGCAAAGGAGGGATGTCAC 6 0.15 No Hit CTACACCCCCATCCTCAATTTCTATATCCTAACCCTAACCCTAAATCCCC 6 0.15 No Hit GGACATTGGGGACGTTGGTTCCCCAAAGCTGAGAAAGTCCAGGAGGTCAC 6 0.15 No Hit GTGGATGATGAAATTACCAGAGTGGAATCCTTGCAATATAACTTAGAAAT 6 0.15 No Hit CTTGAACCAGAGAAGAAGAAGAGTTACTTTGAGCAAGCCAAGGACATGAT 6 0.15 No Hit GGGGAGTGGGACGACTATTCAAAGTATCTGAACCCAGAAGGAGTAGATAT 6 0.15 No Hit GGTTGAAGCAAAAACTGGAAGACAGACAGAGTCCAGTGGTCGAGAGGAAG 6 0.15 No Hit GGATAGGATGGATCTTCTTCGCGCAGCCATTGTTGGTTCAAATGGAACTC 6 0.15 No Hit AGGAGATGTTCAGGCGTGTGAGTGAGCAATTCACAGCTATGTTCAGGAGG 6 0.15 No Hit CCCTGGTTTAATGAGGTCCCACTATCAAAATCAAAAGAATTCATGCCTAC 6 0.15 No Hit GTCTTCTCTTCATTCGAAAAACAAAACCTTCTCCCACCACTCCGCAACCA 5 0.125 No Hit AGGTCATTCATATTGAAGAAATATAACTTCCAATCCATGTTGTAATTATG 5 0.125 No Hit GTCCGATGACCATTTGCCTCCACTTGCTCCGTCTTCTTTCTCCGCAGACA 5 0.125 No Hit CAGAGAAGGGTCACTTTGCGGGAGCTGCTGAGACGGTCGTGGGAGCCGAG 5 0.125 No Hit CTTCCACATCGAGACCAGAACAGGACCTGTCCTTCCTCTGCATCATAATA 5 0.125 No Hit AAGAGCAGTAGCCCTAGAAGGCTATTATGGAACACAACAGGGAGTGCCAG 5 0.125 No Hit AATGTGCAGGAAGGGGTTGCTATTGAAATAGCAGAAGCTGTTGTCGGAGC 5 0.125 No Hit TGATGATCGACACTCAGAGCTGATTCCTTGTCTAGACCGAAATCTTATAT 5 0.125 No Hit TATGAGAGCAAGGGAGCGCACAGCCACAAGGGGTCGAGTTGGTGATAGAA 5 0.125 No Hit AGGAGAGGGAGTTCTTGGAAATGAGGCCAGCAACCAACCCATCAGTAGGA 5 0.125 No Hit GTTGTAGTCAATGAGTCGAGAATTTGGTGTCTTATATTTATAGGACATTT 5 0.125 No Hit CATGCACCACCATCCGGTCCACCACACCGTGAACCTCCTCCTCCAGGTCC 5 0.125 No Hit GGTATGATGAAATTGTCAAGGAGGTGTCATCCTACTTGAAGAAGGTTGGT 5 0.125 No Hit CTCAATTCCAATGGTAGGCGGCTTCGAAGTAGGTGCCGTCCCTTTCAATC 5 0.125 No Hit TGAGAGATGGGAATTTGCCAATGAAGATTTTGTGAAAGATCAAAAGCACC 5 0.125 No Hit GGGAGAGAGTGAAATGAAGTCAGCAATATCATCAACGACAACACTCTTGA 5 0.125 No Hit GCAATTGTTGCTCCTTGGAAATATTGAAGCTTGAGTGTCCAAGGCTGACT 5 0.125 No Hit ATGGAATCATTGGTAAAGGGTCCTTCTACTACAAGTCCATGCACCATGCC 5 0.125 No Hit TTTGATCATAAAATTCTCCACTTCTCTGCATTTGCCATTTGGTCATTCTC 5 0.125 No Hit TGATAACTTGTTTACGGGTCCAATTCCAGGGAGTTTGGTTAATTTGAGTA 5 0.125 No Hit GAAGGTTCCTACATGGGCATCACAGGTTTTGCGGCAGCTGGCCCCAGATG 5 0.125 No Hit GCATGATTGTTCATCCAACTCCTACCAGAGCAGAGGTATCAGACATTGCC 5 0.125 No Hit CTTTTCAGGGAGGATCTGGCACGTATATATGCTGCTGAGATTGTTTCTGC 5 0.125 No Hit AGCAATTGATCGTGGAGAATGACTTGCCCCAAAAACTTCTGAACTTGATC 5 0.125 No Hit GCTCCTGCAAATGAGAAAACGTCGCCGCATGGCTCTAACCAAGCAGTTTT 5 0.125 No Hit CGAAAGTCAACCTAGAAAAATGGCATCGAGATCCGCACTGAGCCGACTTA 5 0.125 No Hit TGAAAAACCACTAAGCACGGCAAAACTGAAAGAAATGAGTGGCAGTGACA 5 0.125 No Hit GGGTTTTCTGCGCTTAGCCTAAAGCAGTTCACTCTGAAAAGCGCCGTTGC 5 0.125 No Hit GTTACAACCATGCATGTTGTTGTTCATCCATCTTCTGTAGAGAAAGAGAA 5 0.125 No Hit CCATATGCAGGTGGGGTGTTTCTAGTAACCATCCATTTCCCTCCTGATTA 5 0.125 No Hit AGGATTTCAAAAGCATTCCTCGAAGACAAAAAGTTTTCCCACCTGATAAG 5 0.125 No Hit GTGCTCATTACAATGAGCTCTATAATTCCCAAGATGAAGTGAAACTCTAC 5 0.125 No Hit GAGCTTGAGTGCATTCTTCTAAGTAAAAGAAATCCCTTAATTTCATCACA 5 0.125 No Hit CTTGTCATTTGTTGCCGAGATTTTTATCTTCCTTTATGTTGGTATGGATG 5 0.125 No Hit AGATGACAATGAAGTTTCCTTGCATGGTAGCAGTTTGGTAGATGACAAAG 5 0.125 No Hit GCTGATTATCACCACAAGAATAACCGGGTTTATTGCTGGAAAGGTCTCCG 5 0.125 No Hit ATCCAGGACAAGGAGGGAATCCCACCAGACCAGCAGAGGCTGATTTTCGC 5 0.125 No Hit AAAATGATTGCTCCAATCTTCGCCGAGTTGGCGAAGAAGTTTCCCAATGT 5 0.125 No Hit GCCAATTTGATGACTGTGACTCCGACCATAGGATATATGATACAACCATC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0125 0.0 20-21 0.0 0.0 0.0 0.025 0.0 22-23 0.0 0.0 0.0 0.025 0.0 24-25 0.0 0.0 0.0 0.025 0.0 26-27 0.0 0.0 0.0 0.025 0.0 28-29 0.0 0.0 0.0 0.025 0.0 30-31 0.0 0.0 0.0 0.025 0.0 32-33 0.0 0.0 0.0 0.025 0.0 34-35 0.0 0.0 0.0 0.025 0.0 36-37 0.0 0.0 0.0 0.025 0.0 38-39 0.0 0.0 0.0 0.025 0.0 40-41 0.0 0.0 0.0 0.025 0.0 42-43 0.0 0.0 0.0 0.025 0.0 44-45 0.0 0.0 0.0 0.025 0.0 46-47 0.0 0.0 0.0 0.025 0.0 48-49 0.0 0.0 0.0 0.025 0.0 50-51 0.0 0.0 0.0 0.025 0.0 52-53 0.0 0.0 0.0 0.025 0.0 54-55 0.0 0.0 0.0 0.025 0.0 56-57 0.0 0.0 0.0 0.025 0.0 58-59 0.0 0.0 0.0 0.025 0.0 60-61 0.0 0.0 0.0 0.025 0.0 62-63 0.0 0.0 0.0 0.025 0.0 64-65 0.0125 0.0 0.0 0.025 0.0 66-67 0.037500000000000006 0.0 0.0 0.025 0.0 68-69 0.05 0.0 0.0 0.025 0.0 70-71 0.0625 0.0 0.0 0.025 0.0 72-73 0.0875 0.0 0.0 0.025 0.0 74-75 0.1 0.0 0.0 0.025 0.0 76-77 0.16249999999999998 0.0 0.0 0.025 0.0 78-79 0.21250000000000002 0.0 0.0 0.025 0.0 80-81 0.225 0.0 0.0 0.025 0.0 82-83 0.225 0.0 0.0 0.025 0.0 84-85 0.25 0.0 0.0 0.025 0.0 86-87 0.275 0.0 0.0 0.025 0.0 88-89 0.3375 0.0 0.0 0.025 0.0 90-91 0.4375 0.0 0.0 0.025 0.0 92-93 0.55 0.0 0.0 0.025 0.0 94-95 0.6000000000000001 0.0 0.0 0.025 0.0 96-97 0.825 0.0 0.0 0.025 0.0 98-99 0.975 0.0 0.0 0.025 0.0 100-101 1.2125 0.0 0.0 0.025 0.0 102-103 1.5375 0.0 0.0 0.025 0.0 104-105 1.975 0.0 0.0 0.025 0.0 106-107 2.1624999999999996 0.0 0.0 0.025 0.0 108-109 2.525 0.0 0.0 0.025 0.0 110-111 2.7375 0.0 0.0 0.025 0.0 112-113 3.125 0.0 0.0 0.025 0.0 114-115 3.4625 0.0 0.0 0.025 0.0 116-117 3.6500000000000004 0.0 0.0 0.025 0.0 118-119 4.0375 0.0 0.0 0.025 0.0 120-121 4.375 0.0 0.0 0.025 0.0 122-123 4.8375 0.0 0.0 0.025 0.0 124-125 5.237500000000001 0.0 0.0 0.025 0.0 126-127 5.9875 0.0 0.0 0.025 0.0 128-129 6.425000000000001 0.0 0.0 0.025 0.0 130-131 6.75 0.0 0.0 0.025 0.0 132-133 7.4625 0.0 0.0 0.025 0.0 134-135 7.9125 0.0 0.0 0.025 0.0 136-137 8.775 0.0 0.0 0.025 0.0 138-139 9.3625 0.0 0.0 0.025 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTGGGT 10 0.006830828 145.0 6 TCAAAGA 10 0.006830828 145.0 3 ATCAAAG 10 0.006830828 145.0 2 >>END_MODULE Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490709 spots for SRR26075343.sra Written 2490709 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra Read 2490696 spots for SRR26075343.sra Written 2490696 spots for SRR26075343.sra SRR ids: ['SRR26075343.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_oe_dti2v SRR26075343.sra spots: 49813933 blocks: [[1, 2490696], [2490697, 4981392], [4981393, 7472088], [7472089, 9962784], [9962785, 12453480], [12453481, 14944176], [14944177, 17434872], [17434873, 19925568], [19925569, 22416264], [22416265, 24906960], [24906961, 27397656], [27397657, 29888352], [29888353, 32379048], [32379049, 34869744], [34869745, 37360440], [37360441, 39851136], [39851137, 42341832], [42341833, 44832528], [44832529, 47323224], [47323225, 49813933]] SRR26075343 file size 18400119 SRR26075343 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075343 SRR26075343_1.fastq SRR26075343_2.fastq Input file: SRR26075343_1.fastq Paired file: SRR26075343_2.fastq trimmed: SRR26075343-trimmed-pair1.fastq, SRR26075343-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 22:16:00 2025 >> started Tue Feb 11 22:17:23 2025 >> done (83.130s) 49813933 read pairs processed; of these: 226 ( 0.00%) short read pairs filtered out after trimming by size control 140744 ( 0.28%) empty read pairs filtered out after trimming by size control 49672963 (99.72%) read pairs available; of these: 7045546 (14.18%) trimmed read pairs available after processing 42627417 (85.82%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 12 0.00% 19 20 0.00% 20 28 0.00% 21 27 0.00% 22 39 0.00% 23 52 0.00% 24 55 0.00% 25 58 0.00% 26 57 0.00% 27 61 0.00% 28 74 0.00% 29 76 0.00% 30 79 0.00% 31 55 0.00% 32 82 0.00% 33 86 0.00% 34 82 0.00% 35 79 0.00% 36 86 0.00% 37 104 0.00% 38 113 0.00% 39 102 0.00% 40 152 0.00% 41 110 0.00% 42 159 0.00% 43 140 0.00% 44 158 0.00% 45 182 0.00% 46 194 0.00% 47 221 0.00% 48 222 0.00% 49 288 0.00% 50 269 0.00% 51 364 0.00% 52 379 0.00% 53 421 0.00% 54 372 0.00% 55 451 0.00% 56 471 0.00% 57 611 0.00% 58 737 0.00% 59 842 0.00% 60 872 0.00% 61 1041 0.00% 62 1235 0.00% 63 1275 0.00% 64 1580 0.00% 65 1632 0.00% 66 1862 0.00% 67 2109 0.00% 68 2347 0.00% 69 2735 0.01% 70 3207 0.01% 71 3731 0.01% 72 4103 0.01% 73 5006 0.01% 74 5580 0.01% 75 6179 0.01% 76 6721 0.01% 77 7635 0.02% 78 8141 0.02% 79 9295 0.02% 80 10624 0.02% 81 12102 0.02% 82 13725 0.03% 83 15121 0.03% 84 16746 0.03% 85 17913 0.04% 86 19757 0.04% 87 21204 0.04% 88 22546 0.05% 89 23984 0.05% 90 26233 0.05% 91 29214 0.06% 92 31112 0.06% 93 33920 0.07% 94 37139 0.07% 95 39443 0.08% 96 40873 0.08% 97 44189 0.09% 98 45284 0.09% 99 47704 0.10% 100 50056 0.10% 101 53084 0.11% 102 56504 0.11% 103 59803 0.12% 104 63510 0.13% 105 66091 0.13% 106 70004 0.14% 107 70806 0.14% 108 74727 0.15% 109 75725 0.15% 110 76870 0.15% 111 81379 0.16% 112 84662 0.17% 113 87149 0.18% 114 92785 0.19% 115 97218 0.20% 116 97784 0.20% 117 102199 0.21% 118 104264 0.21% 119 106342 0.21% 120 108683 0.22% 121 111793 0.23% 122 115134 0.23% 123 118118 0.24% 124 124935 0.25% 125 126541 0.25% 126 132146 0.27% 127 133473 0.27% 128 136275 0.27% 129 138310 0.28% 130 139716 0.28% 131 141775 0.29% 132 146362 0.29% 133 149745 0.30% 134 154304 0.31% 135 158793 0.32% 136 162282 0.33% 137 162010 0.33% 138 166689 0.34% 139 168480 0.34% 140 170472 0.34% 141 173708 0.35% 142 176933 0.36% 143 180200 0.36% 144 185618 0.37% 145 187852 0.38% 146 190180 0.38% 147 192010 0.39% 148 194938 0.39% 149 195468 0.39% 150 198352 0.40% 151 42627417 85.82% 49672963 reads passed initial QC criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=7.29 fanout-score-rank=16 prefix-density=0.68 prefix-fanout=3.7 sequence=TTGCAGCCATTCTCAGCACCAAAGTTCATCTCAGAGCTCTCGTAGAACATCCTAACTGGAGC criterion=fanout-score sequence-density=0.03 sequence-density-rank=34 fanout-score=59.06 fanout-score-rank=1 prefix-density=0.22 prefix-fanout=7.9 sequence=AATCAGCAATTCAGGAAGAAATATGACAAGGAGTAGTAGTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGTGGTAGAATTACTGGCACTCCAATGATTCCATATAACGGCCATAATGGAGCTATAGAATACAACACCAACGTCGCAAAAAACCAGCAAAAATTCTTAACATTATTTTTAGAAATCCCATACTGCCACCGAATATTCAGTCCTTTAAGAAATCGAACAGCATACCCAACATAGTAAAAACCATCAATAATGCAAATACCGTTACCACAAGTGCAAATACTCCCATTCCTACC criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=3.58 fanout-score-rank=20 prefix-density=0.47 prefix-fanout=3.0 sequence=TGCAAGTGCGGCAGTGGCTGCAATGGATGCAGCATGTACCCAGACTTGAGTTTCTCCGAGACCACCACAAGTCAGACAATCATT criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=126.32 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=6.3 sequence=CAAAGCAGTTGCATTTATCTAAAGTATTCTCACTTTACTTAACACTTGAGCTACATAGAATGTCTACAGTCAATTTGGCAGCACTGCTGTTGCTTGGGCTGCTGCTGGTTATGCCACAGCAGTCCATGCAAGCGAGTTTGATAGACCCCATTGCTGAAATCGAGAGAAGCAACTGCAAAATCGCACACCTTCGCTTAGGGCTTGTTTTTACGTCTGATAACAACGAAAGGGCTCTGCAAGACTCTGGACTCTATAGCCCTGACAGTGAAGACTCTTCTGTTGACATTGCGGGCAGAAGGTTCCATAGCGGGACACTAAACGGTTCTTCTATTGTATATGTCAAGACAGGGAGTCATTCAGTAAATATGGCAACAACTTTGCAAATCCTTCTAGTTAGATTCAGCATTCACGGAGTCATCTATTTTGGGAATGCTGGAAGCTTGGATAAGAAAACAATG SRR26075343 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 22:18:17 Started mapping on | Feb 11 22:18:17 Finished on | Feb 11 22:27:48 Mapping speed, Million of reads per hour | 313.17 Number of input reads | 49672963 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 43221646 Uniquely mapped reads % | 87.01% Average mapped length | 293.27 Number of splices: Total | 40360828 Number of splices: Annotated (sjdb) | 39392091 Number of splices: GT/AG | 39634731 Number of splices: GC/AG | 553014 Number of splices: AT/AC | 38423 Number of splices: Non-canonical | 134660 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 2.92 Insertion rate per base | 0.02% Insertion average length | 2.62 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1180701 % of reads mapped to multiple loci | 2.38% Number of reads mapped to too many loci | 129729 % of reads mapped to too many loci | 0.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 9.76% % of reads unmapped: other | 0.59% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 5270616 5270616 5270616 N_multimapping 1180701 1180701 1180701 N_noFeature 1260674 42690448 1562763 N_ambiguous 472833 3257 242127 UnstrandedReadsAssigned:41488139 PositiveStrandReadsAssigned:527941 NegativeStrandReadsAssigned:41416756 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075343 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075343-trimmed-pair1.fastq SRR26075343-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 49,672,963 reads, 42,118,086 reads pseudoaligned [quant] estimated average fragment length: 218.646 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,100 rounds 52401 SRR26075343.ke.tsv 34699 SRR26075343.se.tsv 87100 total ==> SRR26075343.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1800.35 4111 51.8224 Potri.005G024800.1.v4.1 1035 817.354 1689 46.8972 Potri.004G059700.1.v4.1 961 743.369 43 1.31278 Potri.007G009000.2.v4.1 1416 1198.35 0 0 Potri.003G141000.2.v4.1 2943 2725.35 1744.3 14.5253 Potri.016G087400.1.v4.1 270 88.7443 3019 772.058 Potri.015G069301.1.v4.1 564 349.46 0 0 Potri.010G195200.1.v4.1 1773 1555.35 1031.33 15.0485 Potri.012G127500.1.v4.1 977 759.369 16696 498.984 ==> SRR26075343.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 702 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 936 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 5 Potri.001G452600.v4.1 1959 SRR26075343 completed mapping pipeline successfully