Starting /dee2/code/volunteer_pipeline.sh SRR26075344 current disk space = 3052602306560 free memory = 1450268984 SRR26075344 SRAfilesize cff8565ef76742305ba3bae8fc6b838e SRR26075344.sra SRR26075344.sra file validated SRR26075344 is paired end SRR26075344 is conventional basespace SRR26075344 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075344_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.49325 37.0 37.0 37.0 37.0 37.0 2 36.602 37.0 37.0 37.0 37.0 37.0 3 36.6765 37.0 37.0 37.0 37.0 37.0 4 36.589 37.0 37.0 37.0 37.0 37.0 5 36.63 37.0 37.0 37.0 37.0 37.0 6 36.661 37.0 37.0 37.0 37.0 37.0 7 36.619 37.0 37.0 37.0 37.0 37.0 8 36.668 37.0 37.0 37.0 37.0 37.0 9 36.582 37.0 37.0 37.0 37.0 37.0 10-14 36.61045 37.0 37.0 37.0 37.0 37.0 15-19 36.6204 37.0 37.0 37.0 37.0 37.0 20-24 36.5616 37.0 37.0 37.0 37.0 37.0 25-29 36.515299999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.4702 37.0 37.0 37.0 37.0 37.0 35-39 36.427200000000006 37.0 37.0 37.0 37.0 37.0 40-44 36.3715 37.0 37.0 37.0 37.0 37.0 45-49 36.1588 37.0 37.0 37.0 37.0 37.0 50-54 36.2039 37.0 37.0 37.0 37.0 37.0 55-59 36.00359999999999 37.0 37.0 37.0 37.0 37.0 60-64 35.961 37.0 37.0 37.0 37.0 37.0 65-69 35.8605 37.0 37.0 37.0 37.0 37.0 70-74 36.038399999999996 37.0 37.0 37.0 37.0 37.0 75-79 36.0484 37.0 37.0 37.0 37.0 37.0 80-84 36.053900000000006 37.0 37.0 37.0 37.0 37.0 85-89 35.9789 37.0 37.0 37.0 37.0 37.0 90-94 35.9095 37.0 37.0 37.0 37.0 37.0 95-99 35.9589 37.0 37.0 37.0 37.0 37.0 100-104 35.839600000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.8175 37.0 37.0 37.0 37.0 37.0 110-114 35.689099999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.516999999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.6137 37.0 37.0 37.0 37.0 37.0 125-129 35.502500000000005 37.0 37.0 37.0 37.0 37.0 130-134 35.2677 37.0 37.0 37.0 34.6 37.0 135-139 35.1365 37.0 37.0 37.0 27.4 37.0 140-144 35.159000000000006 37.0 37.0 37.0 29.8 37.0 145-149 35.06830000000001 37.0 37.0 37.0 25.0 37.0 150-151 34.8725 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 0.0 23 1.0 24 6.0 25 3.0 26 11.0 27 15.0 28 18.0 29 23.0 30 29.0 31 39.0 32 55.0 33 147.0 34 181.0 35 490.0 36 2793.0 37 187.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.025795141497625 14.099674430252943 11.82068620085149 45.053844227397946 2 18.7 14.45 33.275 33.575 3 17.875 14.499999999999998 27.05 40.575 4 20.349999999999998 22.5 25.35 31.8 5 24.825 27.025 26.174999999999997 21.975 6 23.325000000000003 29.75 23.7 23.225 7 15.0 27.85 39.6 17.549999999999997 8 20.375 29.549999999999997 28.050000000000004 22.025 9 19.1 21.575 36.375 22.95 10-14 19.67098354917746 29.066453322666135 27.466373318665934 23.796189809490475 15-19 19.625 27.415 28.125 24.834999999999997 20-24 19.81 28.455000000000002 28.46 23.275000000000002 25-29 20.255000000000003 28.799999999999997 26.805 24.14 30-34 19.03 27.229999999999997 28.225 25.515 35-39 20.075000000000003 28.499999999999996 27.66 23.765 40-44 19.28 27.275 28.985 24.46 45-49 19.715 27.639999999999997 28.910000000000004 23.735 50-54 20.835 26.345000000000002 28.22 24.6 55-59 20.94 27.175 27.894999999999996 23.990000000000002 60-64 20.79 27.49 27.42 24.3 65-69 20.94 27.744999999999997 27.755000000000003 23.56 70-74 22.27 26.645000000000003 26.810000000000002 24.275 75-79 21.89 27.639999999999997 26.82 23.65 80-84 22.13 26.21 28.285 23.375 85-89 21.88 27.089999999999996 27.400000000000002 23.630000000000003 90-94 21.29 26.44 28.044999999999998 24.224999999999998 95-99 21.77 27.01 27.02 24.2 100-104 22.09 27.125 26.590000000000003 24.195 105-109 21.884999999999998 25.929999999999996 27.485 24.7 110-114 21.765 26.08 27.425 24.73 115-119 22.43 26.88 27.185 23.505000000000003 120-124 23.165 26.145000000000003 27.474999999999998 23.215 125-129 22.545 27.905 25.740000000000002 23.810000000000002 130-134 22.48 27.29 26.69 23.54 135-139 22.175 26.71 26.840000000000003 24.275 140-144 23.03 26.215 27.450000000000003 23.305 145-149 22.689999999999998 26.96 26.395000000000003 23.955000000000002 150-151 23.275000000000002 26.6 26.424999999999997 23.7 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 0.5 19 0.0 20 0.5 21 0.5 22 1.0 23 2.5 24 5.0 25 4.5 26 1.5 27 4.5 28 10.5 29 10.5 30 14.5 31 18.5 32 24.0 33 38.5 34 45.0 35 48.0 36 60.5 37 84.5 38 104.0 39 133.5 40 184.5 41 231.5 42 255.0 43 259.5 44 253.5 45 248.0 46 274.0 47 283.5 48 229.5 49 176.0 50 159.0 51 145.5 52 123.0 53 105.0 54 88.5 55 64.0 56 46.5 57 43.0 58 35.5 59 29.5 60 25.0 61 14.5 62 7.5 63 8.0 64 9.0 65 11.5 66 17.0 67 19.5 68 17.0 69 10.0 70 5.5 71 2.5 72 1.0 73 0.5 74 0.5 75 1.0 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17500000000000002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.005 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.4 #Duplication Level Percentage of deduplicated Percentage of total 1 64.19701986754967 38.775 2 21.150662251655632 25.55 3 7.28476821192053 13.200000000000001 4 3.80794701986755 9.2 5 2.06953642384106 6.25 6 0.6208609271523179 2.25 7 0.37251655629139074 1.575 8 0.20695364238410596 1.0 9 0.08278145695364239 0.44999999999999996 >10 0.20695364238410596 1.7500000000000002 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGAGTATCTCGTAT 25 0.625 TruSeq Adapter, Index 9 (97% over 37bp) GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGAGTATCGCGTAT 13 0.325 TruSeq Adapter, Index 9 (97% over 37bp) GGTTTGATCGCTGTCACTTGAGATGAAGATCACCTCAAATGCATTGTCTT 11 0.27499999999999997 No Hit GTGGGCGGCAGCGGCTGGGAGAGAGGAGAAGGGGAGCTCTGGTTTCTGTC 11 0.27499999999999997 No Hit CTCTGATGTTGGATACTGTGTAGGCTGGTGTTGCACTAATCTCTCATCTT 10 0.25 No Hit AACACCAATTTTGGTGGTTTCACTGTCACTGTAACCCCTTTCGGAGCTTG 9 0.22499999999999998 No Hit ATTTGGAGTATCCTGATCTACACATCTAACATAATAATATGGGGGCATAT 9 0.22499999999999998 No Hit ATTGAAAGTAGCTGCCAGAAAGAAGAGCATAAATACAAGCACTCCTGTCA 8 0.2 No Hit CTCAGCACCGAAGTCCATCTCAGACCTCTCATAGAACATCTTAACTGGTG 8 0.2 No Hit TCCCTGCATGCGGTGAAGATCACCGTCACCTGCAACAAGAGAAAACGCAA 8 0.2 No Hit GGTGAGTATACAGGTTCTCCGGTTTCCAACATGGTTAAATTATCAGCAGG 8 0.2 No Hit GTCTTTGGGATTGACACAACATCCATGAAACCAGATGGGTAAGTTTTATC 8 0.2 No Hit GGACGGCATAAGAGTTGCGGATATCAACGGTGCCATCGGGCAAGACGGAG 7 0.17500000000000002 No Hit CCAGTGCGAGCCCAGTTCATCAAACTATCAACCTTGGAAATCACAAACTC 7 0.17500000000000002 No Hit CCTGAGAGGTGAAGTTGGCAGCCTCCTTGGCAGGATCATCCTTAGAGTTG 7 0.17500000000000002 No Hit AGAGGACACACTTGGCGGGTTTTGAGCCACCGGCTGATACAGTGGAAGTG 7 0.17500000000000002 No Hit CTGTACACAATTACAGGCTTTTTAAATATATCCTCTGTCAAGTATCTTTC 7 0.17500000000000002 No Hit CCCAGTCTCAATGGAAGAAGCACGAGCAGGATATGCAGAAGCAGAAACAG 7 0.17500000000000002 No Hit CGGGTGTTTCAGCTGCGAGAGCTTCTGGGACTGCAAAAGTTGTGTATCTT 7 0.17500000000000002 No Hit TGCATCAATCAAAGAGCTCATATAACCAATAACTAAGTAATTACAGTTGA 7 0.17500000000000002 No Hit TGTCATCATTGCTGTAATAGGAGCAGCACCACAACGCCACTTGTTCGAGG 7 0.17500000000000002 No Hit GTCCATTCCAAATAATGGTTTTGGTTGTATCCAATGCCTCACTGAATGTC 6 0.15 No Hit CTGGCCTTGCTTCTTGATGCCCTGCCCTAACATAGCCATTATCTCCCCAC 6 0.15 No Hit CACCTCCTCATCTGTCAGCTTCTCCCCAAGATTTGTCATGACATGGCGCA 6 0.15 No Hit CCTTAACAAGTGATTGAACCCATGATAGATCAGGCTCGTCTCCATTACCA 6 0.15 No Hit CTCATCTTCTTCATCCTCCTCGCTGTCACTGCTAATTATCTCTTCTTTGT 6 0.15 No Hit ACCAGCTTCCTTCAAATACTCGCGTACAGCCGCGTACTCCTCAATTGCCC 6 0.15 No Hit GCTGGATCTTGCTGGTGGGCTCAGGCATCTCCAAAGTCTTTGTTTGTGAG 6 0.15 No Hit CCGGAGAAAGTATCTTTATATACCAAACATTGTTCACAAATAACTCCCAG 6 0.15 No Hit CTGGCTTATTTAGCTGCCTACAAATCTGAACAATCTTTTGTTGGGCTGAC 6 0.15 No Hit CCCCAGATTGCAAGGGGTCTCATATAAATAAGGGACCGAAAGTGCCCATC 6 0.15 No Hit CTGGTAGAGTGTCATCAGAAAAGCAAATCTTCCCATCCACAGGCACAGAT 6 0.15 No Hit GCTGCTAGTTGAATCAAGAACGCACTTTTTTTCTTCAAAGCATTTACATT 6 0.15 No Hit CACCATGACACTCTTTACCACATTATCACCTGATTCACCAACTTCCACGT 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGAGTATCTCGTTT 6 0.15 TruSeq Adapter, Index 9 (97% over 37bp) CACTAGCAACAACCCAATCTTCACATGGGTTCCATGAAAAATCTGATATT 6 0.15 No Hit GTTGGTTTTTCATCAACCTCCATGGCCTTCACTCGTGAATCATACTCCGA 5 0.125 No Hit CAACCATTTCATGAAACGTATCTCTCAACCATTCATAATCACACAGTCTG 5 0.125 No Hit GTGGGCTTAATATATTCATCTTTTCTTTGTGTTTCATCATAGTTGGCATT 5 0.125 No Hit CCCACCACCCTTCTTCTTTGTCTTTGTACCCAAAGCCAACCAGGCCTTGA 5 0.125 No Hit GCTGGATATATAGCACGTTGAAGGGAACTGGGTTCCAGTGCACTAAAAAG 5 0.125 No Hit CTCTGTTTGATTGCTTCCTCCTCTCTCGTTTCAGCTCCCGGTCATTCGGT 5 0.125 No Hit CTCACACGTAGTCTACAAATATTGATCAATCTTTTAACCAATTATTTTCT 5 0.125 No Hit CACTTGTCGGGTATTATCTTCCCAAATAAGCGAGGTTGAAAATGATACCC 5 0.125 No Hit GCTAAAGAGTTGAATAGCGTCTTTATAGTTCAACCTCTCAACCTTGTATG 5 0.125 No Hit CAGGTTCATTACAAGATCGATCATAAAAGCATAATAAAAAAAAAAATTGG 5 0.125 No Hit CCCTGAAGTTTTTCCGGCGAGTGACGTCGGATACCGGTAGTCTTGAAGAA 5 0.125 No Hit TGCAGACAAGCAAATATGAAAGGCACATTGAAATTCACACGACAACTTTA 5 0.125 No Hit AACCCGCATTCCAAATGGAAAAGGAACCAAAACTAAGTAGACATGCTATT 5 0.125 No Hit GCCATCTTGAAAGTTAATACCAAATTTGGTAGCTAGTTCAACTTTCTCCC 5 0.125 No Hit GCAGCTCTTGTCCTTCAATGGCAATGCCAACCAAGGCATTGTCTCAAAAC 5 0.125 No Hit ATCAGATATAAAAGCATTAAAATTAAACTAAAAAAACTTAACAAATCGAT 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCGAGTATCGCGTTT 5 0.125 TruSeq Adapter, Index 9 (97% over 37bp) CTCGTATGTACAAGAAGAGTTTCGCCCAGTAGCCAATCCTCTCTTGAAAC 5 0.125 No Hit TTTTTTTTCCTTAATTTTGCAGATGAATAATATAGCTTTGATAACTATAT 5 0.125 No Hit ATTTGGAAGGGCCATCAAAGGAGAGAATAATCCTGGGTCCTTGAAACTCT 5 0.125 No Hit CTCTCATCTCTGTAACTCCAAATCTACGAGGCTTCTCTGGTCGATCTTCG 5 0.125 No Hit CGCCAGTTTTGAAGATTCTCCCCAGGAACATATGGCCTTATCTCTGGAGG 5 0.125 No Hit GTCCAAAAGTCAGCCTAAAAATACCACCATATGTGAGGTAAAGCTCATAC 5 0.125 No Hit CACTACCATCAGACCATCTCACGAAGCGTGCATTGCTTTCAACCGAATTC 5 0.125 No Hit GATCATTCAAGTCCATGTTTTTCAGGAGCTCAAGAGTCATAATTTTTGGT 5 0.125 No Hit CTGGAGGGGTCGGCTGGGGAAGAAACCAGAAGAGAATCAGCTCCCGTTTT 5 0.125 No Hit CTCCCCCTCTTATATAATCAAAAATTTGCTGCATTGAAGGTTTCACCAAT 5 0.125 No Hit CCTTGACAATTCTTTTGTTGGATCCAAAGGCACCTCAGGCATCTCATGCA 5 0.125 No Hit TCTAGGATTCACAGACTCATCAGGAACAACAGTTGGGTTAGTTCTCAAAC 5 0.125 No Hit CTTTCTGCATCAATAAACATAAGGTGGTGCTCTAATGAAGAGAGCTGGAT 5 0.125 No Hit CCCCAGTGAGTAACCCTCAGGATCATACATTTCCCAGAATCCTTTGATAG 5 0.125 No Hit GGGTAGAGTTCAGGGGCAGATGTAGAGGAATTTCCAATCCATGATTTATC 5 0.125 No Hit CTCCAAGGCCTTGCACTGATCAAGGGACAGGGAGTCTGGGAAATCAACAG 5 0.125 No Hit CACGATTTGTGTTCTTGTTGCCTTGAATATTAGTGTCATAGATTGACATA 5 0.125 No Hit TCATAACTCACCGGGTCCCACCCACCCATGACCACGAGCTTCCCTTCACA 5 0.125 No Hit TGGTGCCTCCAAGGGCTCGTAGAGCATCTTGGTTTGGATCATAGAGTCGC 5 0.125 No Hit TGCATATAGTTCTGTAAATTCTGAGTGAATTCTTCAATGTTAAGTTTGAT 5 0.125 No Hit GTGTGACAGTCAAGGACAGGGGCATATCCATTCCCAATTTGACCAGGGTG 5 0.125 No Hit ACTACTTATTGACAAACATCAGTCCACCCAAGAAACAAATAACACAATAG 5 0.125 No Hit CAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGA 5 0.125 No Hit GCCTCATTTTCCAACATATATTTCCATTGATAAGCAACTCCTTCCTCTGC 5 0.125 No Hit AGCTTGTGGTGTTGAGAAAGAGACATGTAGCTCGTAATTTGGAACATCTC 5 0.125 No Hit CCCACAGGTACAGTTCCAATACCACCAATCTTGTAGACATCCTGAAGTGG 5 0.125 No Hit CTGGCAAACATAACTAAAAGCAATCTCAAAACAAAATCTTAACAAAACTT 5 0.125 No Hit ACAGCCTCAAAATCCATTCTTTCATCTTTGTCTTCCCCTCAAAGTCAGGT 5 0.125 No Hit GTTTGCTTGAAGCCATCAATGGCTATAGGGACACCTCTACTGTGAAGTTG 5 0.125 No Hit TTCAAATTCACCCAGCTTTGTTTGACATCCTCCTGCACAATTCAAACTTC 5 0.125 No Hit CCTCCTTTTCATGGGAATGTTATCAGGCCTACAGTTGCAAACAAAATAGA 5 0.125 No Hit GTCGTCTAGAGATATTAGGACTACTTCGAAGTTCTCTCTTTTTTCCTTGA 5 0.125 No Hit GTCCTGTAGTATCCTTCCAACGCTTTTCCCTCCTGCGCGTATATTCGAGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.025 0.0 0.0 0.0 0.0 40-41 0.025 0.0 0.0 0.0 0.0 42-43 0.05 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.0625 0.0 0.0 0.0 0.0 50-51 0.075 0.0 0.0 0.0 0.0 52-53 0.075 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.1375 0.0 0.0 0.0 0.0 66-67 0.16249999999999998 0.0 0.0 0.0 0.0 68-69 0.2 0.0 0.0 0.0 0.0 70-71 0.2 0.0 0.0 0.0 0.0 72-73 0.23750000000000002 0.0 0.0 0.0 0.0 74-75 0.275 0.0 0.0 0.0 0.0 76-77 0.3125 0.0 0.0 0.0 0.0 78-79 0.3375 0.0 0.0 0.0 0.0 80-81 0.3625 0.0 0.0 0.0 0.0 82-83 0.5125 0.0 0.0 0.0 0.0 84-85 0.6000000000000001 0.0 0.0 0.0 0.0 86-87 0.7375 0.0 0.0 0.0 0.0 88-89 0.825 0.0 0.0 0.0 0.0 90-91 0.925 0.0 0.0 0.0 0.0 92-93 1.175 0.0 0.0 0.0 0.0 94-95 1.3250000000000002 0.0 0.0 0.0 0.0 96-97 1.475 0.0 0.0 0.0 0.0 98-99 1.5125 0.0 0.0 0.0 0.0 100-101 2.0125 0.0 0.0 0.0 0.0 102-103 2.3 0.0 0.0 0.0 0.0 104-105 2.6875 0.0 0.0 0.0 0.0 106-107 3.0625 0.0 0.0 0.0 0.0 108-109 3.2625 0.0 0.0 0.0 0.0 110-111 3.45 0.0 0.0 0.0 0.0 112-113 3.8125 0.0 0.0 0.0 0.0 114-115 4.275 0.0 0.0 0.0 0.0 116-117 4.7875 0.0 0.0 0.0 0.0 118-119 5.25 0.0 0.0 0.0 0.0 120-121 5.55 0.0 0.0 0.0 0.0 122-123 6.1875 0.0 0.0 0.0 0.0 124-125 6.6875 0.0 0.0 0.0 0.0 126-127 7.3625 0.0 0.0 0.0 0.0 128-129 7.9375 0.0 0.0 0.0 0.0 130-131 8.425 0.0 0.0 0.0 0.0 132-133 8.962499999999999 0.0 0.0 0.0 0.0 134-135 9.725000000000001 0.0 0.0 0.0 0.0 136-137 10.3125 0.0 0.0 0.0 0.0 138-139 10.7125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR26075344 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075344_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.114 37.0 37.0 37.0 37.0 37.0 2 36.275 37.0 37.0 37.0 37.0 37.0 3 36.1825 37.0 37.0 37.0 37.0 37.0 4 36.3055 37.0 37.0 37.0 37.0 37.0 5 36.155 37.0 37.0 37.0 37.0 37.0 6 36.0585 37.0 37.0 37.0 37.0 37.0 7 36.0095 37.0 37.0 37.0 37.0 37.0 8 36.093 37.0 37.0 37.0 37.0 37.0 9 36.1675 37.0 37.0 37.0 37.0 37.0 10-14 36.150999999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.06229999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.00789999999999 37.0 37.0 37.0 37.0 37.0 25-29 35.8406 37.0 37.0 37.0 37.0 37.0 30-34 35.649899999999995 37.0 37.0 37.0 37.0 37.0 35-39 35.6304 37.0 37.0 37.0 37.0 37.0 40-44 35.5573 37.0 37.0 37.0 37.0 37.0 45-49 35.390499999999996 37.0 37.0 37.0 37.0 37.0 50-54 35.2868 37.0 37.0 37.0 37.0 37.0 55-59 35.4197 37.0 37.0 37.0 37.0 37.0 60-64 35.4585 37.0 37.0 37.0 37.0 37.0 65-69 35.403400000000005 37.0 37.0 37.0 37.0 37.0 70-74 35.3277 37.0 37.0 37.0 37.0 37.0 75-79 35.1434 37.0 37.0 37.0 32.2 37.0 80-84 35.197 37.0 37.0 37.0 34.6 37.0 85-89 35.230000000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.0943 37.0 37.0 37.0 27.4 37.0 95-99 35.2842 37.0 37.0 37.0 37.0 37.0 100-104 35.1923 37.0 37.0 37.0 32.2 37.0 105-109 35.1923 37.0 37.0 37.0 29.8 37.0 110-114 35.196600000000004 37.0 37.0 37.0 32.2 37.0 115-119 35.1576 37.0 37.0 37.0 32.2 37.0 120-124 35.0678 37.0 37.0 37.0 25.0 37.0 125-129 35.1167 37.0 37.0 37.0 27.4 37.0 130-134 35.0517 37.0 37.0 37.0 25.0 37.0 135-139 34.881299999999996 37.0 37.0 37.0 25.0 37.0 140-144 34.871050000000004 37.0 37.0 37.0 25.0 37.0 145-149 34.80655 37.0 37.0 37.0 25.0 37.0 150-151 34.357625 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 5.0 14 4.0 15 4.0 16 6.0 17 10.0 18 6.0 19 8.0 20 3.0 21 7.0 22 16.0 23 10.0 24 16.0 25 27.0 26 18.0 27 29.0 28 22.0 29 26.0 30 29.0 31 33.0 32 62.0 33 107.0 34 217.0 35 709.0 36 2464.0 37 162.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.65 19.35 16.950000000000003 31.05 2 29.375 26.950000000000003 25.900000000000002 17.775 3 22.475 27.1 30.475 19.950000000000003 4 24.425 31.225 22.625 21.725 5 28.249999999999996 32.45 22.45 16.85 6 23.9 37.4 20.9 17.8 7 23.7 22.525000000000002 34.275 19.5 8 24.275 26.3 26.474999999999998 22.95 9 24.75 25.15 27.800000000000004 22.3 10-14 25.655 28.625 25.019999999999996 20.7 15-19 25.155 27.705000000000002 26.729999999999997 20.41 20-24 25.715 28.444999999999997 25.575 20.265 25-29 25.005 28.84 25.650000000000002 20.505000000000003 30-34 25.145 27.675 26.179999999999996 21.0 35-39 25.405 28.610000000000003 25.580000000000002 20.405 40-44 25.435000000000002 28.51 25.355 20.7 45-49 25.069999999999997 27.875 26.05 21.005 50-54 23.91 28.975 26.435 20.68 55-59 25.0 27.365000000000002 27.145000000000003 20.49 60-64 25.064999999999998 27.495000000000005 26.915 20.525 65-69 26.290000000000003 29.285 24.715 19.71 70-74 24.895 28.37 25.580000000000002 21.154999999999998 75-79 24.66 29.459999999999997 26.375 19.505 80-84 25.35 28.315 25.52 20.815 85-89 24.740000000000002 28.189999999999998 26.14 20.93 90-94 26.06 28.310000000000002 25.655 19.975 95-99 25.695 29.125 25.405 19.775000000000002 100-104 26.729999999999997 27.805000000000003 24.955 20.51 105-109 25.82 28.88 25.53 19.77 110-114 26.44 27.455000000000002 25.8 20.305 115-119 26.279999999999998 28.854999999999997 25.53 19.335 120-124 26.125 28.110000000000003 26.52 19.245 125-129 26.69 27.800000000000004 25.545 19.965 130-134 27.13 28.244999999999997 25.66 18.965 135-139 27.1 28.255000000000003 24.85 19.794999999999998 140-144 26.55132756637832 28.521426071303562 25.131256562828142 19.795989799489973 145-149 27.33910086512977 28.174226133920087 25.558833825073762 18.927839175876382 150-151 27.428428553569194 26.828353544193025 26.89086135766971 18.85235654456807 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.5 6 0.5 7 0.0 8 0.5 9 1.5 10 2.0 11 1.5 12 0.5 13 0.5 14 1.0 15 0.5 16 1.0 17 1.5 18 1.5 19 1.0 20 3.0 21 4.0 22 2.0 23 2.0 24 2.0 25 2.0 26 3.5 27 5.5 28 7.5 29 6.0 30 8.0 31 9.0 32 13.5 33 25.5 34 29.0 35 39.5 36 63.0 37 92.0 38 108.5 39 123.5 40 151.5 41 203.0 42 244.0 43 257.5 44 274.5 45 297.5 46 298.5 47 267.0 48 245.0 49 204.0 50 158.0 51 140.5 52 119.5 53 95.5 54 85.5 55 64.5 56 47.0 57 38.5 58 29.0 59 33.0 60 29.0 61 19.0 62 12.0 63 11.0 64 9.0 65 6.0 66 5.5 67 2.5 68 1.0 69 2.0 70 4.0 71 3.0 72 1.0 73 1.0 74 1.0 75 2.0 76 2.5 77 2.0 78 2.0 79 3.5 80 4.5 81 5.0 82 4.0 83 3.0 84 4.0 85 4.0 86 3.0 87 3.5 88 3.5 89 4.0 90 4.5 91 2.0 92 1.0 93 1.0 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 1.0 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.005 145-149 0.015 150-151 0.0125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.8 #Duplication Level Percentage of deduplicated Percentage of total 1 67.47611464968153 42.375 2 19.30732484076433 24.25 3 6.687898089171974 12.6 4 3.3837579617834392 8.5 5 1.7117834394904459 5.375 6 0.5971337579617835 2.25 7 0.3582802547770701 1.575 8 0.19904458598726116 1.0 9 0.07961783439490447 0.44999999999999996 >10 0.19904458598726116 1.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 20 0.5 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 13 0.325 No Hit AATTGAAAGGGCAAAACTGGAATCGCAGACGCTTGAGTCAGTTTTGGTTA 11 0.27499999999999997 No Hit GGCAGGGGCCCACGGCTCCCCAATCTCACTTCCTTCTCTCACAGCCATCA 11 0.27499999999999997 No Hit GGGGTGCTGGCATCACTTGGTTTGTGGCAAAAAGAAGCCAAGATCTTGTT 10 0.25 No Hit CAAACTGAGAAAGCTGTGTCACACAGAAATAGTCACAGTAGGACCGGCAA 9 0.22499999999999998 No Hit GTGAACTTTTTGTGTGGAATTGGATACGGGCCTAGAGTAATTCAGGTGAT 9 0.22499999999999998 No Hit AACTTGGTGGTGCATTTGCACCCAAGCCCTCATCTGGACCCCACAAATCT 8 0.2 No Hit TGATCTTGTACCCTGATCCAGGAAGCAGTGCATCATCAACTTCTCTGCTG 8 0.2 No Hit AGATCTGACACCTGAAAAAGAAGGAAAGAACAGCAAATCAGAACAGAAAA 8 0.2 No Hit GCTTGAGCAAATTCAGTTTCTAAGCAAAAGCTTTCCAGGCCCCTTTATCC 8 0.2 No Hit GGCCACTCAATCAATAGAATCAGTAATGAGGCATGCTGGTTCATGTTCTG 8 0.2 No Hit CTTCGGCCCCACTGGACTGACCACTGAAGTTAAATCTGTTGAGATGCACC 7 0.17500000000000002 No Hit CTTCATCGACCCTCTCCGCGAAGGTTCACCCTCTTGTCATCTTCAACATC 7 0.17500000000000002 No Hit GTCTCTAGACGACATAATAGAAAACAACAGGAAGTCGTCGAATTCTCGAG 7 0.17500000000000002 No Hit CAAACCCAGATGGACACGGACGTGACGATGGTTCCGGCAGGCGAGGCAAG 7 0.17500000000000002 No Hit CTGGTGTAAATAACGTGTTCACTTATTATTCATTGCCTTGCCGAACCCCT 7 0.17500000000000002 No Hit AATCGAATGTGGGTGTGTGCACTTTTGGATTAGAAACACAGATACTATCA 7 0.17500000000000002 No Hit GGACTGACCACTGAAGTTAAATCTGTTGAGATGCACCATGAAGCTCTTCA 7 0.17500000000000002 No Hit CAGGCGGCTGAAAGAAGGGACATATAAGGGGAAGAAGTTCAATGCTATAT 7 0.17500000000000002 No Hit TGATCACCAGAAACACCGCCACACGCCTCCCACACCTCCTCTCCATCCAT 7 0.17500000000000002 No Hit TCTCTCTGGAATGGAAGACAAGGCATTCACTACTGCTGAAGGCATTTTCA 6 0.15 No Hit GCTTGCTATTGGAGAAGCAATGTCTGATGCAGAGCTTCACCCAAGCGCAC 6 0.15 No Hit CAGTGACATAGCTGTCATTGCAAAAATAGAGAGTATTGACTCATTGAGGA 6 0.15 No Hit GAAAGAAATACACAATGGCAGGAATCATGCACAAGATTGAGGAGACTCTG 6 0.15 No Hit GGTTTTCCAAGTCGGGTGGAATCCAAAGAATGAGACTATCTTAGCTTCTT 6 0.15 No Hit AAAAACTACTGATACTAGTGTTGTTGTTAGCAAGGATTCGAAAGCGGACC 6 0.15 No Hit GTTCAAAGGGTTGTTATCAGAAATGCCAGAGAAAGAGTTTTTGGAATTGA 6 0.15 No Hit GAGACAAGTGAGTGAAATAAGATTGCTAGAGCAGGAGACAACAGCACCAG 6 0.15 No Hit CAGAACTCCAGGACATGATAAACGAGGTCGATGCTGATGGGAATGGTACC 6 0.15 No Hit AATGCTGCTGCTATTGTTGGCTCCCCAGTTAACACTTGGTCAAAATGGGG 6 0.15 No Hit CCACTGAACCCACCGAAGGGTTTTATTATCTCACAAGATCTGTCATGGCT 6 0.15 No Hit GAGGATGATGATGATGATCTAGATGAAGTGTACCTGAGCAGTTCATCCAA 6 0.15 No Hit TGACCATTGGATTTGTGATAGCTACCGGCTTTTGGCTGTTTTTTCCACCA 6 0.15 No Hit GTGAGAAGCAAGATGATTTATGCTAGTTCTAAGGACAGGTTCAAGAGAGA 6 0.15 No Hit TGCTCTATCATGGTTCATCTACATGTTGTGCAAGCATCCAGCTGTGCAGA 6 0.15 No Hit ATTTTTGATACTAATGGTAAGGTTTCCTGCGATAATGGAGTCAGACATGT 5 0.125 No Hit GAAACCTACTCTTCCCTATCCGCTCTGCATCTCCAAAAACCCCCAAGAAG 5 0.125 No Hit TAGAAGAAGCCGGACACTTCTCCTGAGACAGCAAGGAAGAATAATAGGAA 5 0.125 No Hit GATTTCCATAATGCTACTCTTTGGGCTCCTACTAGCTAGCCTAGATACAA 5 0.125 No Hit CAGCTATTAAGAAACCAAAGAGGTTGACGTCTGTCGTATGGAATCACTTC 5 0.125 No Hit TCACAAACTCTCCAGAACGTGCAAAGCAGAATCATGCCAAGCACCCTAGA 5 0.125 No Hit GCAAGAATTGCAGCCTAGCAGGACGATAGATGATCCTGAAATCAAGGCCT 5 0.125 No Hit AAGAAGCAAGTTAGGGAGCTAAATGAAAAGTTGCAGCTGGCGGAACAAGG 5 0.125 No Hit GACAATTATCATTGAGGACTACGTCCATACTCCAAAAGGCTTGATAAATT 5 0.125 No Hit CATCTGGCAAGCAAGCTCTTCAGGATGCATTAAGGTCTACGCTGGAAGCT 5 0.125 No Hit GCACCAGACCACTTAACATCAAAGATATTGACGTCGGAGAGGGGAGCAGT 5 0.125 No Hit CAAGCATTTGATGAAGTTTTGCAATAGCCTGCATGGTGGATTGAACAAGC 5 0.125 No Hit TATCATTGAAGAACCATCATCACCAGAACCAGTGTCTACACAAGTAACAG 5 0.125 No Hit CCACATTCTGGGCTCAAACTTGTCACTTGTTGATCTTTAGCATCTCATCA 5 0.125 No Hit CTTCATCCCTGTCAAAGATAGAGGCTCTGTGGCTTAAAGAGAGTGGCCAG 5 0.125 No Hit CGCCACCAAGGTGGAAAAATCAAGGAAGCAGCTAAAGGAAAGGAAGAACA 5 0.125 No Hit CTGCAATTCAGCTGGATACTTGCTGGAATTTTGTTGCTCACAGGGTGTAT 5 0.125 No Hit GTGGTAGAAGATCAAGCAGGCCACCATCTCGGGATAGAAGCTTTGGAGGC 5 0.125 No Hit TCGGGGGCTCGCCTTCATCATCCAACTACTCTTCAAACAACAATCGGTTG 5 0.125 No Hit TTTGTGAGGGAGAGACTTCGTCGTATAAGGGGTTTAATTGTTCTGGATAA 5 0.125 No Hit CTAGCATTTTTTATGATGATTTACACTCCAAACGTGTCAAAGGCCACTAT 5 0.125 No Hit GAAATCGTGAAGGAAGTTTCTTCCTACCTGAAGAAGGTTGGTTACAACCC 5 0.125 No Hit CTTTGGGATACTGTTTCATCGAATACAACACTCCACAGGAAGCTGAGCTA 5 0.125 No Hit TATAAATTTAGTAGCATTCTAGCTTGTGCTTCTTCTTCAAATGGGAGAGA 5 0.125 No Hit GGCTGAAGTAGTTGTGCCAATTACTAAGAAACCTGGTCCGCCCAAAGAAT 5 0.125 No Hit GGTGAAAATGGGTTTATTTTGGATGGAATTCCCCGAACGAGAATTCAGGC 5 0.125 No Hit AAAGACCACTACTCTTCCTCTCTATAATTTTCTAGGGTTTAGCAATGTCT 5 0.125 No Hit GCTTCCAGCCAAAAAGACCACTGTGAAGCACAAGAACTTGAACCCTGAAT 5 0.125 No Hit ACGCCGGCCGCCCTCTCTACTGGAAAATCTCCGCCACTGTTCAAGAGATC 5 0.125 No Hit TTCCAAGCCAAAGTACAGGCATGAATTCTACCAAAAGCCAGAAGAGGTGG 5 0.125 No Hit CTTGTTGACCGGACTAGTACATGGTGTGAAACACAAAAGCAGCAAGACCA 5 0.125 No Hit GAGCACACCCTCTCTCTTGCAGAGGCACTCTGTGGCTTCCAGTTCATTTT 5 0.125 No Hit AGAAGATTAAACTCAACAAGCAGCTGCCTTACCTAGTTGGCAACATTGTT 5 0.125 No Hit CAAGAGGGCAGTGGAGGGAGAACTTCGAAGGTGGCGTGAACGAGAGCAAA 5 0.125 No Hit AGACTGGCCCTGGTCATGAAGAGTGGGAAATACACCCTGGGGTACAAGAC 5 0.125 No Hit CAGCATCACTTGCTCTCTTTCTTGCACTCAACCTCCTCTTCTTTTCCCTA 5 0.125 No Hit AATGCTAAGAAGAATCAAACCATAAGCTCTATCTTGGTTTCAAGCTCACG 5 0.125 No Hit GTTGCCAGAGCGTTTTTATTTGAAGGCAGCCAAGCTCTGCAGTGAGCATA 5 0.125 No Hit CGTAATCCTCACTGCTCAGTATAATGATGCCTCTAAAATTGTCCAGTTTA 5 0.125 No Hit CCCAACCAATTTGATCCTAAGACGTATGTGGAAGAGAAAACATTTGTGAC 5 0.125 No Hit CTCCTCGATACCTCTGATATGTATGGACCCCACACCAACGAAATCCTTCT 5 0.125 No Hit GTTCCAGTGATGGAAGTGATGGGAATACAGCTAGGGGAAAGAAAAGGAGC 5 0.125 No Hit AGCAACCCCACCTTCTTTCCATTTTCCAGCCGACAGGAGGCCCCTCCCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.025 0.0 0.0 0.0 0.0 2 0.025 0.0 0.0 0.0 0.0 3 0.025 0.0 0.0 0.0 0.0 4 0.025 0.0 0.0 0.0 0.0 5 0.025 0.0 0.0 0.0 0.0 6 0.025 0.0 0.0 0.0 0.0 7 0.025 0.0 0.0 0.0 0.0 8 0.025 0.0 0.0 0.0 0.0 9 0.025 0.0 0.0 0.0 0.0 10-11 0.025 0.0 0.0 0.0 0.0 12-13 0.025 0.0 0.0 0.0 0.0 14-15 0.025 0.0 0.0 0.0 0.0 16-17 0.025 0.0 0.0 0.0 0.0 18-19 0.025 0.0 0.0 0.0 0.0 20-21 0.025 0.0 0.0 0.0 0.0 22-23 0.025 0.0 0.0 0.0 0.0 24-25 0.025 0.0 0.0 0.0 0.0 26-27 0.025 0.0 0.0 0.0 0.0 28-29 0.025 0.0 0.0 0.0 0.0 30-31 0.025 0.0 0.0 0.0 0.0 32-33 0.025 0.0 0.0 0.0 0.0 34-35 0.025 0.0 0.0 0.0 0.0 36-37 0.025 0.0 0.0 0.0 0.0 38-39 0.05 0.0 0.0 0.0 0.0 40-41 0.05 0.0 0.0 0.0 0.0 42-43 0.075 0.0 0.0 0.0 0.0 44-45 0.075 0.0 0.0 0.0 0.0 46-47 0.075 0.0 0.0 0.0 0.0 48-49 0.075 0.0 0.0 0.0 0.0 50-51 0.075 0.0 0.0 0.0 0.0 52-53 0.075 0.0 0.0 0.0 0.0 54-55 0.075 0.0 0.0 0.0 0.0 56-57 0.075 0.0 0.0 0.0 0.0 58-59 0.075 0.0 0.0 0.0 0.0 60-61 0.1 0.0 0.0 0.0 0.0 62-63 0.125 0.0 0.0 0.0 0.0 64-65 0.1375 0.0 0.0 0.0 0.0 66-67 0.16249999999999998 0.0 0.0 0.0 0.0 68-69 0.2 0.0 0.0 0.0 0.0 70-71 0.2 0.0 0.0 0.0 0.0 72-73 0.23750000000000002 0.0 0.0 0.0 0.0 74-75 0.275 0.0 0.0 0.0 0.0 76-77 0.3125 0.0 0.0 0.0 0.0 78-79 0.3375 0.0 0.0 0.0 0.0 80-81 0.3625 0.0 0.0 0.0 0.0 82-83 0.5125 0.0 0.0 0.0 0.0 84-85 0.6000000000000001 0.0 0.0 0.0 0.0 86-87 0.7375 0.0 0.0 0.0 0.0 88-89 0.825 0.0 0.0 0.0 0.0 90-91 0.925 0.0 0.0 0.0 0.0 92-93 1.175 0.0 0.0 0.0 0.0 94-95 1.375 0.0 0.0 0.0 0.0 96-97 1.525 0.0 0.0 0.0 0.0 98-99 1.5625 0.0 0.0 0.0 0.0 100-101 2.1 0.0 0.0 0.0 0.0 102-103 2.4 0.0 0.0 0.0 0.0 104-105 2.7874999999999996 0.0 0.0 0.0 0.0 106-107 3.1625 0.0 0.0 0.0 0.0 108-109 3.3625 0.0 0.0 0.0 0.0 110-111 3.55 0.0 0.0 0.0 0.0 112-113 3.9125 0.0 0.0 0.0 0.0 114-115 4.375 0.0 0.0 0.0 0.0 116-117 4.887499999999999 0.0 0.0 0.0 0.0 118-119 5.35 0.0 0.0 0.0 0.0 120-121 5.6625 0.0 0.0 0.0 0.0 122-123 6.325 0.0 0.0 0.0 0.0 124-125 6.8375 0.0 0.0 0.0 0.0 126-127 7.5 0.0 0.0 0.0 0.0 128-129 8.0875 0.0 0.0 0.0 0.0 130-131 8.600000000000001 0.0 0.0 0.0 0.0 132-133 9.1375 0.0 0.0 0.0 0.0 134-135 9.899999999999999 0.0 0.0 0.0 0.0 136-137 10.525 0.0 0.0 0.0 0.0 138-139 10.9125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAATGGA 10 0.006830828 145.0 9 AACTCCA 10 0.006830828 145.0 4 GAAGAGT 20 0.00593511 29.0 90-94 >>END_MODULE Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708477 spots for SRR26075344.sra Written 2708477 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra Read 2708466 spots for SRR26075344.sra Written 2708466 spots for SRR26075344.sra SRR ids: ['SRR26075344.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_o60bzmtq SRR26075344.sra spots: 54169331 blocks: [[1, 2708466], [2708467, 5416932], [5416933, 8125398], [8125399, 10833864], [10833865, 13542330], [13542331, 16250796], [16250797, 18959262], [18959263, 21667728], [21667729, 24376194], [24376195, 27084660], [27084661, 29793126], [29793127, 32501592], [32501593, 35210058], [35210059, 37918524], [37918525, 40626990], [40626991, 43335456], [43335457, 46043922], [46043923, 48752388], [48752389, 51460854], [51460855, 54169331]] SRR26075344 file size 20009874 SRR26075344 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075344 SRR26075344_1.fastq SRR26075344_2.fastq Input file: SRR26075344_1.fastq Paired file: SRR26075344_2.fastq trimmed: SRR26075344-trimmed-pair1.fastq, SRR26075344-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 21:38:43 2025 >> started Tue Feb 11 21:39:50 2025 >> done (67.238s) 54169331 read pairs processed; of these: 293 ( 0.00%) short read pairs filtered out after trimming by size control 670592 ( 1.24%) empty read pairs filtered out after trimming by size control 53498446 (98.76%) read pairs available; of these: 7495339 (14.01%) trimmed read pairs available after processing 46003107 (85.99%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 13 0.00% 19 18 0.00% 20 22 0.00% 21 26 0.00% 22 28 0.00% 23 38 0.00% 24 43 0.00% 25 45 0.00% 26 54 0.00% 27 54 0.00% 28 78 0.00% 29 76 0.00% 30 90 0.00% 31 134 0.00% 32 152 0.00% 33 164 0.00% 34 188 0.00% 35 198 0.00% 36 246 0.00% 37 292 0.00% 38 333 0.00% 39 372 0.00% 40 452 0.00% 41 367 0.00% 42 465 0.00% 43 457 0.00% 44 426 0.00% 45 453 0.00% 46 556 0.00% 47 649 0.00% 48 666 0.00% 49 799 0.00% 50 851 0.00% 51 927 0.00% 52 1060 0.00% 53 1198 0.00% 54 1117 0.00% 55 1348 0.00% 56 1359 0.00% 57 1506 0.00% 58 1863 0.00% 59 1823 0.00% 60 2149 0.00% 61 2250 0.00% 62 2717 0.01% 63 3011 0.01% 64 3195 0.01% 65 3288 0.01% 66 3654 0.01% 67 3984 0.01% 68 4054 0.01% 69 4806 0.01% 70 5400 0.01% 71 6220 0.01% 72 6924 0.01% 73 7812 0.01% 74 8868 0.02% 75 9710 0.02% 76 10245 0.02% 77 10784 0.02% 78 12265 0.02% 79 13411 0.03% 80 14663 0.03% 81 15841 0.03% 82 18007 0.03% 83 19975 0.04% 84 21725 0.04% 85 23988 0.04% 86 25547 0.05% 87 27316 0.05% 88 28789 0.05% 89 30851 0.06% 90 32689 0.06% 91 35641 0.07% 92 37564 0.07% 93 40673 0.08% 94 44187 0.08% 95 47206 0.09% 96 50450 0.09% 97 52438 0.10% 98 55221 0.10% 99 56942 0.11% 100 59920 0.11% 101 60704 0.11% 102 63836 0.12% 103 67264 0.13% 104 71293 0.13% 105 74506 0.14% 106 78203 0.15% 107 81202 0.15% 108 82970 0.16% 109 86000 0.16% 110 87744 0.16% 111 89165 0.17% 112 90114 0.17% 113 94602 0.18% 114 97744 0.18% 115 103989 0.19% 116 105780 0.20% 117 108935 0.20% 118 112756 0.21% 119 113053 0.21% 120 114783 0.21% 121 117811 0.22% 122 120001 0.22% 123 121638 0.23% 124 127697 0.24% 125 128993 0.24% 126 134831 0.25% 127 139485 0.26% 128 140827 0.26% 129 142540 0.27% 130 147016 0.27% 131 148976 0.28% 132 150094 0.28% 133 152622 0.29% 134 154167 0.29% 135 157086 0.29% 136 161706 0.30% 137 165401 0.31% 138 169491 0.32% 139 174616 0.33% 140 176107 0.33% 141 178586 0.33% 142 180946 0.34% 143 180309 0.34% 144 184361 0.34% 145 187826 0.35% 146 190698 0.36% 147 192229 0.36% 148 195290 0.37% 149 200691 0.38% 150 203269 0.38% 151 46003107 85.99% 53498446 reads passed initial QC criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=2.08 fanout-score-rank=32 prefix-density=0.24 prefix-fanout=2.0 sequence=TACAGTCCCCAACAAGTTCTCTCCATTCTCTGGATAATTGAAATCTCCAAATACCAGTTTACCTTTCTCCGATCCGAATTTCTTCCAGTTCCAAACTCCTGTGAAGGCAACAGCCTGCGGCACAGAAACATCACCTGGAACCATTGTTTTCTTATCCAAGCTTCCAGCATTCCCAAAATAGATGACTCCGTGAATGCTGAATCTAACTAGAAGGATTTGC criterion=fanout-score sequence-density=0.03 sequence-density-rank=34 fanout-score=88.56 fanout-score-rank=1 prefix-density=0.32 prefix-fanout=8.7 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.22 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=41 prefix-density=0.21 prefix-fanout=2.0 sequence=AAGCGCTCAAGGATATGAAGTTAAGAAAATGCTATTCTGATGAATGCCTGCCCGGAAAACCCAAAGTTGTCTTTGGATCGAAGAGTTCTACTTCTGATTTTTACGTTCGAAATAAAGC criterion=fanout-score sequence-density=0.02 sequence-density-rank=35 fanout-score=98.67 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=11.4 sequence=ACAACAACAAAGAGCTGTCGGAAGAAAGAAATTAGAAGATGCACAATCCCCCACCGCCAGAGCCCCGTCACCAGCCCGGCCA SRR26075344 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 21:40:32 Started mapping on | Feb 11 21:40:32 Finished on | Feb 11 21:49:05 Mapping speed, Million of reads per hour | 375.43 Number of input reads | 53498446 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 48218877 Uniquely mapped reads % | 90.13% Average mapped length | 293.24 Number of splices: Total | 46825367 Number of splices: Annotated (sjdb) | 45689875 Number of splices: GT/AG | 45932992 Number of splices: GC/AG | 686324 Number of splices: AT/AC | 50343 Number of splices: Non-canonical | 155708 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 3.26 Insertion rate per base | 0.02% Insertion average length | 2.72 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1317324 % of reads mapped to multiple loci | 2.46% Number of reads mapped to too many loci | 199192 % of reads mapped to too many loci | 0.37% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.57% % of reads unmapped: other | 0.47% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 3962245 3962245 3962245 N_multimapping 1317324 1317324 1317324 N_noFeature 1226229 47637880 1598703 N_ambiguous 467388 2869 256684 UnstrandedReadsAssigned:46525260 PositiveStrandReadsAssigned:578128 NegativeStrandReadsAssigned:46363490 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075344 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075344-trimmed-pair1.fastq SRR26075344-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 53,498,446 reads, 46,925,068 reads pseudoaligned [quant] estimated average fragment length: 220.027 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,335 rounds 52401 SRR26075344.ke.tsv 34699 SRR26075344.se.tsv 87100 total ==> SRR26075344.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1798.97 4108 45.4334 Potri.005G024800.1.v4.1 1035 815.973 3479 84.8297 Potri.004G059700.1.v4.1 961 741.973 0 0 Potri.007G009000.2.v4.1 1416 1196.97 0 0 Potri.003G141000.2.v4.1 2943 2723.97 2560 18.6985 Potri.016G087400.1.v4.1 270 88.6827 4293.58 963.275 Potri.015G069301.1.v4.1 564 347.779 0 0 Potri.010G195200.1.v4.1 1773 1553.97 1056 13.5204 Potri.012G127500.1.v4.1 977 757.973 22076 579.477 ==> SRR26075344.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 33 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 597 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 4417 SRR26075344 completed mapping pipeline successfully