Starting /dee2/code/volunteer_pipeline.sh SRR26075345 current disk space = 3052572053504 free memory = 1451095612 SRR26075345 SRAfilesize 72ed33965a0702c1ca5e991db18806ff SRR26075345.sra SRR26075345.sra file validated SRR26075345 is paired end SRR26075345 is conventional basespace SRR26075345 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075345_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.66025 37.0 37.0 37.0 37.0 37.0 2 36.638 37.0 37.0 37.0 37.0 37.0 3 36.646 37.0 37.0 37.0 37.0 37.0 4 36.756 37.0 37.0 37.0 37.0 37.0 5 36.6785 37.0 37.0 37.0 37.0 37.0 6 36.6095 37.0 37.0 37.0 37.0 37.0 7 36.692 37.0 37.0 37.0 37.0 37.0 8 36.703 37.0 37.0 37.0 37.0 37.0 9 36.691 37.0 37.0 37.0 37.0 37.0 10-14 36.690999999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.6202 37.0 37.0 37.0 37.0 37.0 20-24 36.583 37.0 37.0 37.0 37.0 37.0 25-29 36.517700000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.468399999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.4259 37.0 37.0 37.0 37.0 37.0 40-44 36.30409999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.0615 37.0 37.0 37.0 37.0 37.0 50-54 36.084199999999996 37.0 37.0 37.0 37.0 37.0 55-59 35.9737 37.0 37.0 37.0 37.0 37.0 60-64 35.9097 37.0 37.0 37.0 37.0 37.0 65-69 35.785000000000004 37.0 37.0 37.0 37.0 37.0 70-74 35.959500000000006 37.0 37.0 37.0 37.0 37.0 75-79 36.0741 37.0 37.0 37.0 37.0 37.0 80-84 35.987300000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.8821 37.0 37.0 37.0 37.0 37.0 90-94 35.8448 37.0 37.0 37.0 37.0 37.0 95-99 35.8106 37.0 37.0 37.0 37.0 37.0 100-104 35.8261 37.0 37.0 37.0 37.0 37.0 105-109 35.729299999999995 37.0 37.0 37.0 37.0 37.0 110-114 35.6649 37.0 37.0 37.0 37.0 37.0 115-119 35.6129 37.0 37.0 37.0 34.6 37.0 120-124 35.605900000000005 37.0 37.0 37.0 37.0 37.0 125-129 35.452 37.0 37.0 37.0 37.0 37.0 130-134 35.274 37.0 37.0 37.0 34.6 37.0 135-139 35.103699999999996 37.0 37.0 37.0 27.4 37.0 140-144 34.9209 37.0 37.0 37.0 27.4 37.0 145-149 34.9089 37.0 37.0 37.0 25.0 37.0 150-151 34.75475 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 1.0 22 7.0 23 2.0 24 6.0 25 9.0 26 9.0 27 10.0 28 16.0 29 19.0 30 47.0 31 43.0 32 53.0 33 165.0 34 153.0 35 465.0 36 2793.0 37 200.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.65314300025044 13.423491109441525 7.162534435261708 34.76083145504633 2 17.549999999999997 14.149999999999999 35.4 32.9 3 17.175 16.875 31.6 34.35 4 21.7 26.075 25.25 26.974999999999998 5 24.45 31.0 25.900000000000002 18.65 6 23.724999999999998 33.025 22.875 20.375 7 14.774999999999999 29.575000000000003 39.275 16.375 8 17.0 27.775 31.25 23.974999999999998 9 18.325 23.075000000000003 33.725 24.875 10-14 20.305 30.005 26.99 22.7 15-19 20.185 27.465 27.83 24.52 20-24 19.74 28.82 27.98 23.46 25-29 20.45 27.565 27.43 24.555 30-34 19.505 27.92 28.560000000000002 24.015 35-39 20.53 28.54 27.025 23.905 40-44 20.175 27.83 28.199999999999996 23.794999999999998 45-49 20.21 27.605 27.48 24.705 50-54 19.31 27.584999999999997 27.955000000000002 25.15 55-59 20.285 28.384999999999998 27.96 23.369999999999997 60-64 21.205 27.165 27.42 24.21 65-69 20.62 28.225 27.43 23.724999999999998 70-74 22.06 27.229999999999997 27.400000000000002 23.31 75-79 21.95 27.075 27.07 23.905 80-84 21.709999999999997 27.029999999999998 27.0 24.26 85-89 20.69 28.125 27.175 24.01 90-94 21.97 27.005000000000003 27.46 23.565 95-99 22.12 26.724999999999998 28.249999999999996 22.905 100-104 21.26 27.735 27.265 23.74 105-109 21.81 27.48 26.490000000000002 24.22 110-114 22.085 27.544999999999998 26.3 24.07 115-119 22.45 27.29 27.075 23.185 120-124 22.52 27.37 26.229999999999997 23.880000000000003 125-129 22.615 27.62 25.805 23.96 130-134 22.835 27.115000000000002 26.345000000000002 23.705000000000002 135-139 22.475 26.965 26.69 23.87 140-144 23.665 26.76 26.334999999999997 23.24 145-149 23.87 27.015 25.435000000000002 23.68 150-151 23.6875 26.174999999999997 24.675 25.4625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 1.0 6 1.5 7 0.5 8 0.0 9 0.0 10 0.5 11 1.0 12 1.0 13 1.5 14 1.5 15 1.0 16 1.0 17 0.5 18 0.0 19 0.0 20 0.5 21 0.5 22 0.5 23 2.0 24 2.5 25 2.5 26 6.0 27 10.0 28 14.0 29 14.5 30 18.0 31 26.5 32 35.5 33 46.5 34 53.5 35 57.0 36 66.5 37 89.0 38 117.5 39 150.5 40 176.5 41 192.0 42 209.0 43 237.5 44 269.0 45 263.0 46 252.5 47 247.5 48 217.0 49 195.5 50 179.0 51 152.5 52 141.5 53 128.5 54 87.0 55 60.5 56 50.5 57 31.5 58 24.0 59 19.0 60 12.5 61 14.0 62 10.0 63 8.0 64 10.5 65 8.0 66 8.0 67 13.5 68 17.0 69 12.5 70 8.0 71 6.0 72 4.0 73 2.5 74 1.5 75 2.5 76 2.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17500000000000002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.675000000000004 #Duplication Level Percentage of deduplicated Percentage of total 1 64.36967977300365 39.7 2 20.30806647750304 25.05 3 8.958248885285771 16.575 4 3.8913660316173493 9.6 5 1.4187271990271584 4.375 6 0.5674908796108634 2.1 7 0.2837454398054317 1.225 8 0.08107012565869477 0.4 9 0.040535062829347386 0.22499999999999998 >10 0.08107012565869477 0.75 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAACCGGTATCTCGTAT 18 0.44999999999999996 TruSeq Adapter, Index 4 (97% over 38bp) CCTGACTGTCAAATGAAAGCTCAAACCCGTAAAGGTAATCATGGTTCAGT 12 0.3 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAACCGGTATCGCGTAT 9 0.22499999999999998 TruSeq Adapter, Index 4 (97% over 38bp) GATGAACTTGGTGTGTCCACGGCCGTGCTTGTTGCGGCCTCCATTCCTTC 8 0.2 No Hit GCCAAGCTTCCATCATCTTTTGGTGTAAGATGATAGGTGGGATTGAATAT 8 0.2 No Hit CGCAGGCTTTGATTATCCTCAAGCAAGTGATCATACTGTAGAAGTGACCC 7 0.17500000000000002 No Hit CACCCTAGCATATCCATGAATCCGGTTTGTCACAGGTTTAACACACACAA 7 0.17500000000000002 No Hit CTCGAGGTTTCGACCGCCTTCTGTACGACGGAAAACAGAGACACCACCAT 7 0.17500000000000002 No Hit GCCTCATTTTGGGCCTCCAGGTCCAGCTCGGGGTCGTGCGACTCACCGAG 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAACCGGTATCGCGGTT 7 0.17500000000000002 TruSeq Adapter, Index 4 (97% over 38bp) GGGTGATACAGAGAGGGGGGATTGATTAAATTTTTAAAAACCAGCAACCA 7 0.17500000000000002 No Hit CTTGAATATCCTCCGCCCTGCACGCTTTTTCGGACGACTTGTTGCTAGTA 7 0.17500000000000002 No Hit CTGCATCATTGGGGCTTGCTAAAATCGCTCTCCCACAGTACTCTTCAGCT 6 0.15 No Hit CTCTGCAACAGAGACTAAAAAACTGGGCGGACACTGTTGTCAAGGAGAAA 6 0.15 No Hit CCGCCAAATTGTCACAACCGATATCCACTTTTGCTGCATTGTCACGGCGA 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAACCGGTATCGCGTTT 6 0.15 TruSeq Adapter, Index 4 (97% over 38bp) GTCGAGATCGATGACCCCTTCTATTACTCCGTGCTAAGGGCTTCGTCGAT 6 0.15 No Hit CGTCAGCATCAGCGCCAACAGATCCAAGGATGTTTTTCAAATCCTCGGCG 6 0.15 No Hit GCCTAACTGAAGATGTTCGTTTTCATTGATCGCTGGAACATATTCATCAA 6 0.15 No Hit CTTTTTAAAATCACTAGATGCGTGTCTTAGAATTGAATCACTTGAATAAC 6 0.15 No Hit GTCCTTATCATCCACAGCTTCATTTTTGAAGACGGCCTTGATATACTACT 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAACCGGTATCTCGTTT 6 0.15 TruSeq Adapter, Index 4 (97% over 38bp) GTTTAGGTTTAGCAGCTGCTGATTTCTTGGCCTTCTTAGCCCCAGCAACT 6 0.15 No Hit CATGTGAATTACTGGGCCTCAATTGTGTTTAAATTGCTAGCACCATCAGC 6 0.15 No Hit CTATGATTATGGTCATTTTCTTCTTTTGTGACTCGTTCAGGTCGACCCCT 6 0.15 No Hit GCCGTTAGCGCAACCTTTAGATGTTCTGAAGCAAAGCCCTGGGCCTCCAT 6 0.15 No Hit GCCTTGTGAACACTTGGGTACTGGGGGGCACCATGGATAACGCTAGGCAT 5 0.125 No Hit CATGTCTATCACCAACAACCGGCAGCATCACCGGAAGCGGCAGAGGAGAA 5 0.125 No Hit TTTTTTTTTTAACAAATGCAATTCACATTATGTTTCAATAATCTTCGTGG 5 0.125 No Hit GCTGTGAACACACCAATCCTTCAGGAAGATCTGTTAGTTTATTGCCCATT 5 0.125 No Hit GCCACATGTTACGGGCTACGGTCTCTGCCTTCTTGCCCCATTCCTGAAAC 5 0.125 No Hit CCTATGTTTTACATGCCTGGATCAGTACGTAGCTTCTTCTTCTTCTTCTT 5 0.125 No Hit CTCCAGAATTCCAAGAAGGAACTAAAAAGTTCCAACAGTGTATTTGATTA 5 0.125 No Hit ACCATGTAAAAGTGGTTCAACAAATTTAAGCCATGCAACACGGCGATCCT 5 0.125 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAACCGGTATCTCGGTT 5 0.125 TruSeq Adapter, Index 4 (97% over 38bp) ATCATATTCAAAACCACACTATGATTGTATACAAATCTTGTAATAGACCT 5 0.125 No Hit CGCGCCCCGGGTTTTGCAGCGACCGCCGCGCCCTCCTACTCATCGGGGCC 5 0.125 No Hit GATCGGGTCAGAGCCTCGACCCGGTACCATGACCTACAAACCAATGACGC 5 0.125 No Hit GTGCTGTCAGTAGGAATTGGTGTGACGTCCTCAATGCGACCAATTTTCAT 5 0.125 No Hit GGCCAACTTCAACTCCTGATGGCACTCAATGCTTTGAGAACATGCCTTGC 5 0.125 No Hit CTCACAAGCAAAAGGGAATGATACAAAAAGCTAACAAAGCAGATAGCATG 5 0.125 No Hit CCTAGATGAAGTTGTTCTCAGCCTTCCCGAGCTATACATGTCATCTCTTA 5 0.125 No Hit TGAAGGAGTTTGGGCGTCGAAATCAGACATGAAAACTTAACTGTTCAAGG 5 0.125 No Hit GCATCAAATATGAAGATGGTCATAGCTGACCAGCGGTATTGAAGTCCTTC 5 0.125 No Hit GTTGATTCTTCACTACTAGCAAGATCCTTGATATTCTTGCCAGAATTGTC 5 0.125 No Hit TGGATGACATACCAGGAGTGCTAATACCTGAGTTGATTGCAAGACATACA 5 0.125 No Hit ACCACCGGGAAAACCTTCAGTCTTGCACACACTAGCACAGTTGTGGCCTC 5 0.125 No Hit CTCTAGGTTGTCAGTGAGAACTGTTACGGTCATGAACTGCGTATGCATTT 5 0.125 No Hit CTCTTTCCTTGTACTCCCATTTTAAAAAAGTACTCGGCAGCTGAGGCAAG 5 0.125 No Hit CCATAGTTCAACTGGTCTTGTTCCCTTGCAGAATTTAACAGGCCAGCTAC 5 0.125 No Hit CCCGGTGTGGCTTGGCTAGAGGAGATATCTCAATAGGATAGTCCAAAACA 5 0.125 No Hit AGGTAGCGTTGGCATTTGTCCAACATTTATGCTGCTAATATAGGTTAACT 5 0.125 No Hit GGGGCACCGTTGTAGGTAGTAAGAGTCCAGATGAATCAATATTTGCACCA 5 0.125 No Hit CCTGAAATAGAAATACCGATGAAGAGAATTTACAGGAAGATGATTGAGAA 5 0.125 No Hit GAAAGAAAGATTCTGGAAGTAGAAAGCGAAACCGACAAACAATTCTTTTG 5 0.125 No Hit CCTTTCTGCAGTTTCTTCAGCAGGTTTGTCTCGGAATTCAGTTGGTGGGC 5 0.125 No Hit ACTAAAACCAACGAGTGACAAAATCTCCATATAATTTGCTAAATACAGAC 5 0.125 No Hit AGCTCATGTAAAGGAACCCATCTTCATCCTTGTTTTCCTCATATATTGCT 5 0.125 No Hit CGCCAACCATTAAAATTGGGTGAATTTTTATGCGGACAATCCACCTCTCC 5 0.125 No Hit CCCATACAACCCATTTTTAAAAACAGCCCTCGGATTAACCCAGTATTACA 5 0.125 No Hit GCCTAAGTGTCTTTGACCTTAACAAATCATGAGCAGACTGCAACGACGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.0625 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.0875 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1125 0.0 0.0 0.0 0.0 76-77 0.1375 0.0 0.0 0.0 0.0 78-79 0.3375 0.0 0.0 0.0 0.0 80-81 0.4875 0.0 0.0 0.0 0.0 82-83 0.5 0.0 0.0 0.0 0.0 84-85 0.5625 0.0 0.0 0.0 0.0 86-87 0.7 0.0 0.0 0.0 0.0 88-89 0.9 0.0 0.0 0.0 0.0 90-91 1.075 0.0 0.0 0.0 0.0 92-93 1.2625 0.0 0.0 0.0 0.0 94-95 1.6124999999999998 0.0 0.0 0.0 0.0 96-97 1.9375 0.0 0.0 0.0 0.0 98-99 2.175 0.0 0.0 0.0 0.0 100-101 2.3 0.0 0.0 0.0 0.0 102-103 2.6 0.0 0.0 0.0 0.0 104-105 2.8125 0.0 0.0 0.0 0.0 106-107 3.1375 0.0 0.0 0.0 0.0 108-109 3.5375 0.0 0.0 0.0 0.0 110-111 3.8625 0.0 0.0 0.0 0.0 112-113 4.225 0.0 0.0 0.0 0.0 114-115 4.6875 0.0 0.0 0.0 0.0 116-117 5.1 0.0 0.0 0.0 0.0 118-119 5.925 0.0 0.0 0.0 0.0 120-121 6.6 0.0 0.0 0.0 0.0 122-123 6.975 0.0 0.0 0.0 0.0 124-125 7.8125 0.0 0.0 0.0 0.0 126-127 8.8 0.0 0.0 0.0 0.0 128-129 9.337499999999999 0.0 0.0 0.0 0.0 130-131 10.1875 0.0 0.0 0.0 0.0 132-133 10.774999999999999 0.0 0.0 0.0 0.0 134-135 11.8875 0.0 0.0 0.0 0.0 136-137 12.7375 0.0 0.0 0.0 0.0 138-139 13.6875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAGAGC 75 0.0013135396 38.666668 6 GAGCACA 75 0.0013135396 38.666668 9 TCGGAAG 80 0.0018040554 36.25 3 AGAGCAC 80 0.0018040554 36.25 8 CGGAAGA 85 0.002429728 34.11765 4 ATCGGAA 85 0.002429728 34.11765 2 GATCGGA 90 0.0032161705 32.22222 1 GGAAGAG 90 0.0032161705 32.22222 5 AAGAGCA 95 0.004191872 30.526318 7 >>END_MODULE SRR26075345 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR26075345_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.98025 37.0 37.0 37.0 37.0 37.0 2 36.3555 37.0 37.0 37.0 37.0 37.0 3 36.2645 37.0 37.0 37.0 37.0 37.0 4 36.2795 37.0 37.0 37.0 37.0 37.0 5 36.336 37.0 37.0 37.0 37.0 37.0 6 36.2005 37.0 37.0 37.0 37.0 37.0 7 36.161 37.0 37.0 37.0 37.0 37.0 8 36.1945 37.0 37.0 37.0 37.0 37.0 9 36.267 37.0 37.0 37.0 37.0 37.0 10-14 36.101099999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.1055 37.0 37.0 37.0 37.0 37.0 20-24 35.935500000000005 37.0 37.0 37.0 37.0 37.0 25-29 35.739000000000004 37.0 37.0 37.0 37.0 37.0 30-34 35.571600000000004 37.0 37.0 37.0 37.0 37.0 35-39 35.4517 37.0 37.0 37.0 37.0 37.0 40-44 35.4596 37.0 37.0 37.0 37.0 37.0 45-49 35.3578 37.0 37.0 37.0 37.0 37.0 50-54 35.2456 37.0 37.0 37.0 37.0 37.0 55-59 35.2895 37.0 37.0 37.0 37.0 37.0 60-64 35.3744 37.0 37.0 37.0 37.0 37.0 65-69 35.269099999999995 37.0 37.0 37.0 37.0 37.0 70-74 35.144600000000004 37.0 37.0 37.0 37.0 37.0 75-79 35.099599999999995 37.0 37.0 37.0 34.6 37.0 80-84 35.1588 37.0 37.0 37.0 34.6 37.0 85-89 35.116200000000006 37.0 37.0 37.0 32.2 37.0 90-94 35.0492 37.0 37.0 37.0 29.8 37.0 95-99 35.2173 37.0 37.0 37.0 34.6 37.0 100-104 35.0755 37.0 37.0 37.0 29.8 37.0 105-109 35.1314 37.0 37.0 37.0 29.8 37.0 110-114 35.0484 37.0 37.0 37.0 27.4 37.0 115-119 35.0581 37.0 37.0 37.0 27.4 37.0 120-124 34.98879999999999 37.0 37.0 37.0 25.0 37.0 125-129 34.9795 37.0 37.0 37.0 25.0 37.0 130-134 34.915 37.0 37.0 37.0 25.0 37.0 135-139 34.69555 37.0 37.0 37.0 25.0 37.0 140-144 34.681200000000004 37.0 37.0 37.0 25.0 37.0 145-149 34.7396 37.0 37.0 37.0 25.0 37.0 150-151 34.24125 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0 1101 2 0.0 1101 3 0.0 1101 4 0.0 1101 5 0.0 1101 6 0.0 1101 7 0.0 1101 8 0.0 1101 9 0.0 1101 10-14 0.0 1101 15-19 0.0 1101 20-24 0.0 1101 25-29 0.0 1101 30-34 0.0 1101 35-39 0.0 1101 40-44 0.0 1101 45-49 0.0 1101 50-54 0.0 1101 55-59 0.0 1101 60-64 0.0 1101 65-69 0.0 1101 70-74 0.0 1101 75-79 0.0 1101 80-84 0.0 1101 85-89 0.0 1101 90-94 0.0 1101 95-99 0.0 1101 100-104 0.0 1101 105-109 0.0 1101 110-114 0.0 1101 115-119 0.0 1101 120-124 0.0 1101 125-129 0.0 1101 130-134 0.0 1101 135-139 0.0 1101 140-144 0.0 1101 145-149 0.0 1101 150-151 0.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 5.0 14 8.0 15 9.0 16 7.0 17 11.0 18 7.0 19 4.0 20 10.0 21 8.0 22 22.0 23 7.0 24 21.0 25 14.0 26 19.0 27 25.0 28 21.0 29 18.0 30 29.0 31 48.0 32 58.0 33 100.0 34 256.0 35 796.0 36 2347.0 37 150.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.01250938203653 20.765574180635475 9.55716787590693 19.664748561421067 2 31.175000000000004 24.075 24.575 20.175 3 25.05 27.400000000000002 30.599999999999998 16.950000000000003 4 28.7 30.925000000000004 22.125 18.25 5 26.450000000000003 36.85 19.35 17.349999999999998 6 23.875 37.775 20.875 17.474999999999998 7 24.95 21.075 34.1 19.875 8 21.75 26.3 26.875 25.074999999999996 9 25.825 23.525 27.6 23.05 10-14 26.115 29.32 24.104999999999997 20.46 15-19 26.484999999999996 27.825 25.705 19.985 20-24 26.334999999999997 28.275 25.35 20.04 25-29 26.455000000000002 28.494999999999997 25.695 19.355 30-34 26.555 27.075 25.69 20.68 35-39 26.135 27.755000000000003 25.874999999999996 20.235 40-44 26.965 27.33 25.6 20.105 45-49 26.31 27.36 26.32 20.01 50-54 24.43 28.389999999999997 27.384999999999998 19.794999999999998 55-59 26.02 28.895 25.695 19.39 60-64 25.575 27.925 25.424999999999997 21.075 65-69 25.91 27.83 26.174999999999997 20.085 70-74 25.6 28.95 25.869999999999997 19.580000000000002 75-79 24.55 28.38 26.715 20.355 80-84 25.09 28.134999999999998 26.755000000000003 20.02 85-89 25.85 28.349999999999998 25.385 20.415 90-94 25.595000000000002 27.755000000000003 26.174999999999997 20.474999999999998 95-99 26.179999999999996 27.97 25.590000000000003 20.26 100-104 26.240000000000002 28.144999999999996 25.715 19.900000000000002 105-109 26.169999999999998 28.455000000000002 26.045 19.33 110-114 26.665 28.27 25.145 19.919999999999998 115-119 26.83 28.7 25.929999999999996 18.54 120-124 27.495000000000005 27.79 25.69 19.025 125-129 26.955000000000002 27.450000000000003 25.900000000000002 19.695 130-134 26.86 28.185 24.97 19.985 135-139 27.589138370755613 27.109066359953992 25.983897584637695 19.3178976846527 140-144 27.87393696848424 27.86893446723362 25.822911455727866 18.434217108554275 145-149 29.361425282754478 27.33960564508057 24.46702031828646 18.831948753878493 150-151 28.114057028514257 28.65182591295648 25.550275137568786 17.68384192096048 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 1.0 10 1.0 11 2.0 12 1.0 13 1.0 14 2.0 15 2.0 16 1.0 17 1.0 18 1.5 19 0.5 20 0.5 21 2.0 22 1.5 23 0.5 24 0.5 25 1.5 26 4.5 27 5.5 28 4.5 29 3.0 30 3.0 31 9.0 32 15.5 33 23.0 34 27.5 35 30.0 36 51.5 37 78.0 38 107.0 39 130.5 40 157.0 41 202.5 42 244.0 43 280.0 44 304.0 45 296.0 46 278.5 47 269.0 48 248.0 49 215.0 50 179.0 51 129.0 52 110.5 53 110.5 54 82.0 55 59.5 56 39.5 57 27.5 58 29.0 59 29.0 60 27.0 61 20.5 62 9.5 63 4.5 64 3.5 65 4.0 66 3.5 67 1.5 68 3.0 69 3.0 70 0.5 71 3.0 72 3.5 73 0.5 74 1.5 75 2.0 76 1.0 77 2.5 78 5.0 79 6.0 80 4.5 81 4.0 82 5.0 83 4.5 84 5.5 85 5.0 86 2.0 87 6.0 88 8.5 89 3.0 90 1.5 91 3.5 92 5.0 93 5.0 94 2.5 95 1.0 96 1.0 97 1.0 98 0.5 99 1.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.015 140-144 0.05 145-149 0.09 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.775000000000006 #Duplication Level Percentage of deduplicated Percentage of total 1 66.54719235364396 41.775 2 19.036240541616888 23.9 3 8.323377140581442 15.675 4 3.823178016726404 9.6 5 1.3142174432497014 4.125 6 0.5973715651135006 2.25 7 0.15929908403026682 0.7000000000000001 8 0.07964954201513341 0.4 9 0.0 0.0 >10 0.11947431302270012 1.575 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 33 0.8250000000000001 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT 19 0.475 No Hit GAGTGAGATATTCAAGAGAAATACGTTTAGAAATCAAGAGAGAGAGAGAG 11 0.27499999999999997 No Hit GAGTGGTTCACGCAGAAGGACAGCGAGAGAAAAGTAAAGGCAAGATTGTT 8 0.2 No Hit GTACAGTGTAGATGGAAAACAGGAGGTCTTTAAAGAGAAGGTGGTAGTGG 8 0.2 No Hit GGAAATCACCAGCTCCGAAACACGGAAGCCCGCTGTGGAGCGCCGGATAT 7 0.17500000000000002 No Hit TCACAAGTCATTCATTATCCGTGCCCAAAAGTTCAAAGCGGACCCATAAA 7 0.17500000000000002 No Hit GAAAAGCTTAGACTCATCCGATTTAGGGTTTCGCTTCATATCAATCAAAT 7 0.17500000000000002 No Hit AAACAAAACCGAAGTTTTGAAGATGGAAAAAATGGAGAGGGCAAAGGTCT 7 0.17500000000000002 No Hit GGTGAATTGATTTGTTGTAGGAATTTATTTTTAGAAGAAGAACAATCGAT 6 0.15 No Hit CCCGAAGAAGCTGCTGAGGTCAATAAATATTGTCTCTCACCTTCGCATAA 6 0.15 No Hit CTCACTGGGAAATGAAAGGTGTTCCTCTGAGGATTGAAATTGGGCCCAAA 6 0.15 No Hit TTCCCACGTTAATTTACTCGGTCGTGAAAGGCTTGAAGATTGGTAAAGGC 6 0.15 No Hit GGAACATTGGAGAAACTATTGATGAGAAAGGTGATACCATTGCCATCTTC 6 0.15 No Hit GGAAGTTTTATGGTGGTGGTGGGTCGGATTTTGGTGATGATAGAGGATCA 6 0.15 No Hit GGAGGAGAGAAAGATAAGGAGACTATGGGGGTAGTTAAAGGAGTGGAAAA 6 0.15 No Hit GGGCATCTTACAAGCTTCCAGAGGCGAAAAAGACTAAAGAGGTTAAACCT 6 0.15 No Hit GAGAGAGGGCAGATATTCAACAAGGTCATGAAGGTAGAGATTCCATCAGA 6 0.15 No Hit AGCGGCCGTGCTGAAGAGCCAGCTCTCTAAATCCCTAAGCTGCCTTCACT 6 0.15 No Hit AGTTACCCCAACAGTCCTGGGTCCCAAAGTATGCACATCAAGAGGTCAAT 6 0.15 No Hit CAAATCTCCAGATGAGCTGCTCGAAGAAACAGATCGAAAGATTGAGATAC 6 0.15 No Hit ACCGCACCGGTGAGGAAGAAACAACATGGCAGTTGTCCCGCTTTTACCCC 6 0.15 No Hit GAGAAAAGACCGCACTTCTAGAAACAAGGAATAATGCATCTCTAAGATCT 6 0.15 No Hit GTGGAAGCCAAGAAGGAGCAATTGGGGTTGCATGATTTTGAAAGGGTTGT 6 0.15 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGT 5 0.125 No Hit GCCGAATCAACTAGCCCCGAAAATGGATGGCGCTGAAGCGCGCGACCTAT 5 0.125 No Hit CATGGCCTCCTTTCTTTCTTCACATTCTCTTCTTCAACTTGTTCTGTTAA 5 0.125 No Hit GTTGCTATTCAGATAGCATCATCAGAAAAATACGGATTCATGGTAGAGGC 5 0.125 No Hit GTTCTTGGTCAAATCCTTTTCGACGTCTGCATTCCCAAGAATGTTGAAGG 5 0.125 No Hit TGTGAATCCACCTCAGTGGTCACAGCAAAGCTGATCTTTGAAGATGGCAA 5 0.125 No Hit CTCAGGGTCAGGGTCAGGGTCAGGGTCGAGGCCGAGGCCGTGGGAAGAAA 5 0.125 No Hit GAAGAACCATGCTAGAGACAGAAGAGCTTGGTGTCTCAATTCTTGAAGAT 5 0.125 No Hit AGTTACCCCTGCTAATGGTGGGACAGACAATGGAGTCGGGAAAACTCAAT 5 0.125 No Hit GGGGAATCCAGACAAGGTCTTCCTCATGTAGCTGGCCTGTTAAATTCTGC 5 0.125 No Hit CTGGACAGAATCAAGAAGGCATATGATAGGAATCCTGATCTTGCCAACCT 5 0.125 No Hit CCAGAAGGAAAATACTCGAAAGAAGTTGTTGCCGTCGACACCGATGGTCA 5 0.125 No Hit CTTGAAGTTGCTAAAGATGTCACCTTGAGGACTCTTGGATCTGGACTTGA 5 0.125 No Hit CCAAGGACTGATAGTAATAGAAGGAGAGCTAGTAAGCGAATACGCATTCC 5 0.125 No Hit GGGAGAAGGTTAGCTGATACTTTCCCTAACCTTTATTGGGCTCCTTGTGC 5 0.125 No Hit TGTCAATCTGACTCATACAGCATAATGAATCCAGCTATGAATCCCTCTCA 5 0.125 No Hit CTTCACCATATGCTGCTATGCTTGCTGCACAGGATGTTTCACAGAGATGC 5 0.125 No Hit GTGGCAAAGATTGTTGACCCATCTATGATCGTTGATGAAGATTTATTGGA 5 0.125 No Hit AAAACATATCTGAAACTGTTGTTGCCCTTGTCACTGAAGAAGGTGCCCAT 5 0.125 No Hit CATGATTCTAGCCCAACCCGGACCTGCACCCCCGGGCCCGGGTCGGGTTC 5 0.125 No Hit TCTGAAGCCAGATGTCATTGCTCCTGGTGTTAACATCTTGGCTGGTTGGA 5 0.125 No Hit AATGTACGGTCCTGATTAGCTGTCCTAATAATCTATTTGATCTTGATCAA 5 0.125 No Hit GAGGACAAGCCTAACAAGAAATATTTTGATCCTCGTGTTTGGGTTCGCGA 5 0.125 No Hit GCATCTATTGCTAGTTTCTCTCAGCGCCTCTTCCAAACTAAAATGAAGGT 5 0.125 No Hit AATCAGCAAAAAATGTCTCAAGGCTCTGCCTACCAAACTAGGCAGCGGCA 5 0.125 No Hit ACCAACCTCATCTGCCACTGTCACGGAGGAATCCCACCCTAATGATCACT 5 0.125 No Hit AGTAGTAGTTGCAGACAGAGAGCTAGTGTTAGACAGGTTCTTGTTAGACA 5 0.125 No Hit ATTTAATCAGCGAGAATCCATTGCTTGTGAAGGCAGAAATTCATGAATGG 5 0.125 No Hit GGCATGTTACGATTCGCGTTTGAAGGCGCTAAGAGAATAGAATGGCGCCT 5 0.125 No Hit ATGACATTGAGATGCCCGAAAGCCCTAACCTTTATGCGGATGTGGATGCG 5 0.125 No Hit GTACTCCTTGTGACTTGTATACAGAGGAGCGATCCTCGCAGCCCTTGGTT 5 0.125 No Hit AGGAGGTTGGGGGCTGGTGGTATGTGGACACCATGCTTAGTGGTGATGCC 5 0.125 No Hit AGCTAGGCAAGATGGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.0625 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.3125 0.0 0.0 0.0 0.0 80-81 0.4625 0.0 0.0 0.0 0.0 82-83 0.475 0.0 0.0 0.0 0.0 84-85 0.5375000000000001 0.0 0.0 0.0 0.0 86-87 0.675 0.0 0.0 0.0 0.0 88-89 0.875 0.0 0.0 0.0 0.0 90-91 1.0375 0.0 0.0 0.0 0.0 92-93 1.2125 0.0 0.0 0.0 0.0 94-95 1.5625 0.0 0.0 0.0 0.0 96-97 1.8875 0.0 0.0 0.0 0.0 98-99 2.1375 0.0 0.0 0.0 0.0 100-101 2.2750000000000004 0.0 0.0 0.0 0.0 102-103 2.6 0.0 0.0 0.0 0.0 104-105 2.8125 0.0 0.0 0.0 0.0 106-107 3.1625 0.0 0.0 0.0 0.0 108-109 3.5875 0.0 0.0 0.0 0.0 110-111 3.9125 0.0 0.0 0.0 0.0 112-113 4.275 0.0 0.0 0.0 0.0 114-115 4.7375 0.0 0.0 0.0 0.0 116-117 5.15 0.0 0.0 0.0 0.0 118-119 5.975 0.0 0.0 0.0 0.0 120-121 6.675 0.0 0.0 0.0 0.0 122-123 7.05 0.0 0.0 0.0 0.0 124-125 7.887499999999999 0.0 0.0 0.0 0.0 126-127 8.9125 0.0 0.0 0.0 0.0 128-129 9.462499999999999 0.0 0.0 0.0 0.0 130-131 10.2875 0.0 0.0 0.0 0.0 132-133 10.9 0.0 0.0 0.0 0.0 134-135 12.0875 0.0 0.0 0.0 0.0 136-137 12.975000000000001 0.0 0.0 0.0 0.0 138-139 13.9375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATATGT 10 0.006830828 145.0 1 TTTGTCA 10 0.006830828 145.0 3 GGGTTTT 20 0.00593511 29.0 45-49 GGGGGGA 35 0.0035366106 20.714287 65-69 >>END_MODULE Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994555 spots for SRR26075345.sra Written 2994555 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra Read 2994542 spots for SRR26075345.sra Written 2994542 spots for SRR26075345.sra SRR ids: ['SRR26075345.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_muep3auh SRR26075345.sra spots: 59890853 blocks: [[1, 2994542], [2994543, 5989084], [5989085, 8983626], [8983627, 11978168], [11978169, 14972710], [14972711, 17967252], [17967253, 20961794], [20961795, 23956336], [23956337, 26950878], [26950879, 29945420], [29945421, 32939962], [32939963, 35934504], [35934505, 38929046], [38929047, 41923588], [41923589, 44918130], [44918131, 47912672], [47912673, 50907214], [50907215, 53901756], [53901757, 56896298], [56896299, 59890853]] SRR26075345 file size 22124504 SRR26075345 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR26075345 SRR26075345_1.fastq SRR26075345_2.fastq Input file: SRR26075345_1.fastq Paired file: SRR26075345_2.fastq trimmed: SRR26075345-trimmed-pair1.fastq, SRR26075345-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Tue Feb 11 21:43:33 2025 >> started Tue Feb 11 21:45:02 2025 >> done (89.138s) 59890853 read pairs processed; of these: 501 ( 0.00%) short read pairs filtered out after trimming by size control 718523 ( 1.20%) empty read pairs filtered out after trimming by size control 59171829 (98.80%) read pairs available; of these: 11033058 (18.65%) trimmed read pairs available after processing 48138771 (81.35%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 42 0.00% 19 86 0.00% 20 53 0.00% 21 89 0.00% 22 100 0.00% 23 95 0.00% 24 93 0.00% 25 132 0.00% 26 155 0.00% 27 208 0.00% 28 143 0.00% 29 178 0.00% 30 173 0.00% 31 185 0.00% 32 164 0.00% 33 205 0.00% 34 220 0.00% 35 222 0.00% 36 245 0.00% 37 260 0.00% 38 308 0.00% 39 241 0.00% 40 388 0.00% 41 361 0.00% 42 330 0.00% 43 387 0.00% 44 411 0.00% 45 416 0.00% 46 546 0.00% 47 538 0.00% 48 676 0.00% 49 710 0.00% 50 755 0.00% 51 827 0.00% 52 1013 0.00% 53 996 0.00% 54 1086 0.00% 55 1293 0.00% 56 1284 0.00% 57 1693 0.00% 58 1947 0.00% 59 2012 0.00% 60 2347 0.00% 61 2858 0.00% 62 3373 0.01% 63 3627 0.01% 64 3887 0.01% 65 4171 0.01% 66 4786 0.01% 67 5085 0.01% 68 5966 0.01% 69 7069 0.01% 70 8265 0.01% 71 9607 0.02% 72 10571 0.02% 73 12375 0.02% 74 13595 0.02% 75 14936 0.03% 76 15874 0.03% 77 17323 0.03% 78 19695 0.03% 79 21537 0.04% 80 24267 0.04% 81 27388 0.05% 82 31467 0.05% 83 34074 0.06% 84 37719 0.06% 85 40491 0.07% 86 41736 0.07% 87 44386 0.08% 88 46765 0.08% 89 50669 0.09% 90 53869 0.09% 91 59216 0.10% 92 63889 0.11% 93 69390 0.12% 94 73167 0.12% 95 76674 0.13% 96 79716 0.13% 97 82934 0.14% 98 83914 0.14% 99 88402 0.15% 100 90786 0.15% 101 96509 0.16% 102 103570 0.18% 103 108558 0.18% 104 114715 0.19% 105 119020 0.20% 106 121322 0.21% 107 122394 0.21% 108 124547 0.21% 109 127377 0.22% 110 128687 0.22% 111 136025 0.23% 112 141996 0.24% 113 147602 0.25% 114 153222 0.26% 115 157674 0.27% 116 161073 0.27% 117 163062 0.28% 118 165302 0.28% 119 166878 0.28% 120 169149 0.29% 121 172691 0.29% 122 178929 0.30% 123 186386 0.31% 124 192835 0.33% 125 197129 0.33% 126 202885 0.34% 127 203384 0.34% 128 205831 0.35% 129 206883 0.35% 130 208328 0.35% 131 209680 0.35% 132 214532 0.36% 133 222697 0.38% 134 226029 0.38% 135 234746 0.40% 136 235910 0.40% 137 237656 0.40% 138 239267 0.40% 139 239471 0.40% 140 243352 0.41% 141 244481 0.41% 142 248900 0.42% 143 253623 0.43% 144 262376 0.44% 145 266511 0.45% 146 268536 0.45% 147 273345 0.46% 148 273809 0.46% 149 271924 0.46% 150 273118 0.46% 151 48138771 81.35% 59171829 reads passed initial QC criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=22.51 fanout-score-rank=5 prefix-density=0.25 prefix-fanout=22.5 sequence=GGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAACCGGTATCTCGTATGCCGTCTTCTGCTTG criterion=fanout-score sequence-density=0.03 sequence-density-rank=28 fanout-score=112.60 fanout-score-rank=1 prefix-density=0.34 prefix-fanout=10.9 sequence=CATCACCATCCCTGATTGATCTTTGATTCACAACAAGACCAACCAACCGTACAATGTTTACATAACTTGGGATAATCTCACAAGAAAGAAAGATCAATACAAAACTAGTCAGCTCAGGATGTTCCAGGCACGGTAGAACCGTAGAACCATAACAACAAGAGACATATTGCAGATGAGTACTGAAAAACAAAACACAGTACGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTCGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTAGGCTGTGGCTTGTCAAAAACATGAGCACCAATATTCTTAGTAGCAAGGTTGCTACCAGGGTGGAACACGCTCCTCCAAACATTGCTACGCGCCGACACTGGGGTTGTAGGGGTCACTGGTGTCGTCGGTGTCCCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACTGCTCCCCTCTCCGACGTCAATATCTTTGATG criterion=sequence-density sequence-density=0.32 sequence-density-rank=1 fanout-score=2.85 fanout-score-rank=28 prefix-density=0.33 prefix-fanout=2.8 sequence=ATGTACCCTGAC criterion=fanout-score sequence-density=0.12 sequence-density-rank=14 fanout-score=34.85 fanout-score-rank=1 prefix-density=0.50 prefix-fanout=8.6 sequence=TTGGTGATGGTGAAGGTTCATCAAAATCTAACTGTAGCATTATGTGAAAGAAACCAATGATGCATTTTATCACCAGCCAAATGCATCCAATCGTACAATAAATATTGCCGATGAGGTAAGAGGAAATGACTGAAAATTTACTGGAAACTCTTCAAGTGGGCTCAGATCCAGTAGAGAATAAACTCAAGTTACTTTATATTGCTGTATCTTGTTTGTGGCAATAATTGTATTCTCATGCATTCGATCTCCATAGTCTCATGTG SRR26075345 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 11 21:45:54 Started mapping on | Feb 11 21:45:55 Finished on | Feb 11 21:56:11 Mapping speed, Million of reads per hour | 345.81 Number of input reads | 59171829 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 51289378 Uniquely mapped reads % | 86.68% Average mapped length | 290.13 Number of splices: Total | 43744233 Number of splices: Annotated (sjdb) | 42667360 Number of splices: GT/AG | 42934177 Number of splices: GC/AG | 619221 Number of splices: AT/AC | 48394 Number of splices: Non-canonical | 142441 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 3.13 Insertion rate per base | 0.02% Insertion average length | 2.53 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 1616279 % of reads mapped to multiple loci | 2.73% Number of reads mapped to too many loci | 627821 % of reads mapped to too many loci | 1.06% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 8.89% % of reads unmapped: other | 0.63% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 6266172 6266172 6266172 N_multimapping 1616279 1616279 1616279 N_noFeature 1323596 50643629 1679790 N_ambiguous 597067 3932 305071 UnstrandedReadsAssigned:49368715 PositiveStrandReadsAssigned:641817 NegativeStrandReadsAssigned:49304517 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR26075345 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR26075345-trimmed-pair1.fastq SRR26075345-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 59,171,829 reads, 51,042,334 reads pseudoaligned [quant] estimated average fragment length: 204.96 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,231 rounds 52401 SRR26075345.ke.tsv 34699 SRR26075345.se.tsv 87100 total ==> SRR26075345.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1814.04 6260 55.387 Potri.005G024800.1.v4.1 1035 831.04 4291.07 82.8752 Potri.004G059700.1.v4.1 961 757.04 114 2.41694 Potri.007G009000.2.v4.1 1416 1212.04 0 0 Potri.003G141000.2.v4.1 2943 2739.04 2547.4 14.9272 Potri.016G087400.1.v4.1 270 94.9564 4922.55 832.045 Potri.015G069301.1.v4.1 564 361.645 0 0 Potri.010G195200.1.v4.1 1773 1569.04 548 5.60567 Potri.012G127500.1.v4.1 977 773.04 36967 767.526 ==> SRR26075345.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 849 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 630 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 10 Potri.001G452600.v4.1 1838 SRR26075345 completed mapping pipeline successfully